Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0000066

Physical Location & Seq

Genomic Coordinates Forward (+)

7439171 .. 7442113

Gene Structure 2943 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G21250.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2208 bp

ATGAAGGTGCAGGAGGGTTTGTTCTTGGTGGCTATTTTCTTCTCCCTTGCGTGTACGCAGCTGGTGAAGGGGCAACATCAACCTGGTGAGAATTGCCAAAATAAATGTGGCAACATCACAATAGAGTACCCTTTTGGCATTTCTTCAGGTTGTTACTATCCCGGAAATGAAAGTTTCAGTATCACCTGTAAGGAAGATAGGCCACATGTCTTAAGCGACATTGAAGTGGCAAACTTTAATCACAGCGGCCAGCTACAAGTTCTGCTTAATCGATCCTCTACTTGCTACGACGAGCAAGGAAAAAAAACTGAGGAGGACAGTTCTTTTACACTGGAAAATTTATCTCTTTCCGCCAACAACAAGTTAACTGCAGTAGGCTGTAACGCTTTATCACTTCTGGACACTTTTGGAATGCAAAACTACTCAACTGCATGCTTGTCATTATGCGATTCTCCCCCAGAGGCTGATGGAGAATGTAATGGTAGAGGTTGCTGCAGAGTCGACGTTTCTGCCCCGTTGGATAGCTATACATTCGAAACTACATCAGGTCGCATCAAGCACATGACTTCTTTTCACGACTTTAGTCCTTGCACCTACGCTTTTCTCGTTGAAGATGATAAGTTCAACTTCAGTTCTACAGAAGATCTTCTGAATCTGCGAAATGTCATGAGGTTCCCTGTGTTACTAGATTGGTCTGTTGGAAATCAGACATGCGAGCAAGTTGGAAGCACAAGCATATGCGGTGGGAACAGCACTTGTCTCGATTCTACTCCTAGAAACGGGTATATCTGCAGATGCAATGAAGGCTTTGATGGGAATCCATACCTTTCAGCTGGTTGCCAAGACGTCAATGAGTGTACTACTAGTAGTACTATCCATAGACATAACTGTTCGGATCCCAAAACCTGTAGAAACAAGGTTGGAGGCTTCTATTGTAAGTGTCAATCTGGTTACCGCTTAGATACCACCACTATGAGCTGCAAGCGTAAAGAGTTTGCATGGACTACAATTCTTCTTGTAACCACCATCGGCTTCTTGGTCATTCTGCTTGGCGTTGCCTGTATACAACAGAGAATGAAGCACCTGAAGGACACCAAGCTCCGAGAACAATTCTTCGAGCAAAATGGTGGCGGCATGTTGACACAACGACTCTCAGGAGCAGGGCCGTCAAATGTTGATGTCAAAATCTTTACTGAGGATGGCATGAAGAAAGCAACAAATGGTTATGCTGAGAGCAGGATCCTGGGTCAGGGTGGCCAAGGAACAGTGTACAAAGGGATATTGCCGGACAACTCCATAGTTGCTATAAAGAAAGCCCGACTTGGAGACAGTAGCCAAGTAGAGCAGTTCATCAATGAAGTGCTCGTGCTTTCACAAATCAACCATAGGAACGTAGTCAAGCTCTTGGGCTGCTGTCTAGAGACTGAAGTTCCCTTGTTGGTCTATGAGTTCATCACCAATGGCACCCTTTTCGATCACTTGCATGGTTCCATGATTGATTCTTCGCTTACATGGGAACACCGTCTGAAGATAGCAATAGAAGTCGCTGGAACTCTTGCATATCTTCACTCCTCTGCTTCTATTCCAATCATCCATCGGGATATCAAAACTGCAAATATTCTTCTGGATGTAAACTTAACTGCAAAAGTAGCTGACTTTGGTGCTTCAAGGCTGATACCAATGGATAAAGAAGAGCTCGAAACTATGGTGCAAGGCACTCTAGGTTACCTAGACCCAGAATATTACAACACAGGGTTGTTAAACGAAAAGAGCGATGTTTATAGTTTTGGGGTCGTCCTAATGGAACTGCTCTCAGGTCAAAAGGCATTGTGCTTTAAACGGCCACAGTCCTCAAAACATCTGGTGAGTTACTTTGCGACTGCCACAAAAGAGAATAGGTTGGATGAGATTATTGGCGGCGAAGTGATGAACGAGGATAATCTGAAGGAGATCCAGGAAGCTGCAAGAATTGCTGCAGAGTGTACAAGGCTAATGGGAGAGGAAAGGCCAAGGATGAAAGAAGTAGCTGCAAAGCTAGAAGCCTTGAGGGTCGAAAAAACCAAACATAAGTGGTCGGATCAGTACCCTGAGGAGAATGAACACTTGATTGGTGGTCACATCTTGTCAGCACAAGGCGAAACCAGTAGCAGCATTGGCTATGACAGCATCAAGAATGTAGCAATATTGGACATTGAAACTGGCCGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000902 GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005886 GO:0005975 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006884 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0007166 GO:0007275 GO:0008150 GO:0008152 GO:0008361 GO:0009311 GO:0009505 GO:0009605 GO:0009607 GO:0009615 GO:0009620 GO:0009653 GO:0009719 GO:0009725 GO:0009751 GO:0009791 GO:0009826 GO:0009987 GO:0009992 GO:0010033 GO:0014070 GO:0016020 GO:0016043 GO:0016049 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019725 GO:0022622 GO:0023052 GO:0030104 GO:0030312 GO:0032501 GO:0032502 GO:0032535 GO:0032989 GO:0036211 GO:0040007 GO:0042221 GO:0042493 GO:0042592 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0046677 GO:0048364 GO:0048527 GO:0048528 GO:0048589 GO:0048731 GO:0048856 GO:0048869 GO:0048878 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055082 GO:0060560 GO:0065007 GO:0065008 GO:0071704 GO:0071840 GO:0071944 GO:0090066 GO:0090696 GO:0098542 GO:0099402 GO:0140096 GO:1901564 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr

Protein Analysis

735

Amino Acids

81.21

Weight (kDa)

5.46

Isoelectric Point (pI)

39.95

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GUB_WAK_bind	PF13947	32 - 84	1.3e-09	Wall-associated receptor kinase galacturonan-binding
EGF_CA	PF07645	282 - 327	3.4e-08	Calcium-binding EGF domain
Pkinase	PF00069	409 - 675	5e-42	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	413 - 679	7.5e-46	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000066)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G21210 AT1G21230 AT1G21240 AT1G21240 AT1G21245 AT1G21250 AT1G21270 AT1G22720 AT3G25490
fragaria_vesca	FvH4_2g35051 FvH4_2g35051 FvH4_2g35060 FvH4_3g10281 FvH4_3g10285 FvH4_3g10286 FvH4_3g10311 FvH4_3g10311 FvH4_3g10320 FvH4_3g10332 FvH4_3g10340 FvH4_3g10350 FvH4_3g10360 FvH4_3g10390 FvH4_3g10412 FvH4_3g10413 FvH4_3g10461 FvH4_3g10462 FvH4_3g10470 FvH4_3g10510 FvH4_3g10520 FvH4_3g10530 FvH4_3g10550 FvH4_3g10560 FvH4_3g10611 FvH4_3g11730 FvH4_6g53140 FvH4_6g53142 FvH4_7g31880 FvH4_7g31910 FvH4_7g31920
malus_domestica	MD05G1269900.v1.1 MD05G1270000.v1.1 MD05G1270200.v1.1 MD05G1270400.v1.1 MD05G1270500.v1.1 MD05G1270600.v1.1 MD05G1270800.v1.1 MD05G1270900.v1.1 MD07G1183800.v1.1 MD08G1107600.v1.1 MD10G1248500.v1.1 MD10G1249200.v1.1 MD10G1249500.v1.1 MD10G1250000.v1.1 MD10G1250500.v1.1 MD10G1250900.v1.1 MD10G1251200.v1.1 MD10G1251400.v1.1 MD13G1004600.v1.1 MD15G1088400.v1.1
prunus_persica	Prupe.1G442400_v2.0.a1 Prupe.1G442500_v2.0.a1 Prupe.2G223400_v2.0.a1 Prupe.4G091900_v2.0.a1 Prupe.4G093200_v2.0.a1 Prupe.4G093300_v2.0.a1 Prupe.4G093400_v2.0.a1 Prupe.4G093500_v2.0.a1 Prupe.4G094100_v2.0.a1 Prupe.4G094200_v2.0.a1 Prupe.4G094300_v2.0.a1 Prupe.6G358300_v2.0.a1
pyrus_communis	pycom08g08990 pycom10g20660 pycom10g20670 pycom10g20680 pycom10g20710 pycom10g20740 pycom13g00400 pycom15g08290
rosa_chinensis	RchiOBHm_Chr1g0326861 RchiOBHm_Chr1g0326871 RchiOBHm_Chr1g0331391 RchiOBHm_Chr1g0336911 RchiOBHm_Chr1g0364001 RchiOBHm_Chr2g0174541 RchiOBHm_Chr2g0175041 RchiOBHm_Chr2g0175101 RchiOBHm_Chr2g0175121 RchiOBHm_Chr3g0486331 RchiOBHm_Chr5g0015581 RchiOBHm_Chr5g0016551 RchiOBHm_Chr5g0016561 RchiOBHm_Chr5g0016581 RchiOBHm_Chr5g0016601 RchiOBHm_Chr5g0016611 RchiOBHm_Chr5g0016641 RchiOBHm_Chr5g0016661 RchiOBHm_Chr5g0016691 RchiOBHm_Chr5g0016701 RchiOBHm_Chr5g0016731 RchiOBHm_Chr5g0016741 RchiOBHm_Chr5g0016811 RchiOBHm_Chr5g0016841 RchiOBHm_Chr5g0016871 RchiOBHm_Chr5g0016881 RchiOBHm_Chr5g0016931 RchiOBHm_Chr5g0017021 RchiOBHm_Chr5g0017041 RchiOBHm_Chr5g0017071 RchiOBHm_Chr5g0017091 RchiOBHm_Chr5g0017121 RchiOBHm_Chr5g0017141 RchiOBHm_Chr5g0017291 RchiOBHm_Chr5g0017321 RchiOBHm_Chr5g0017401 RchiOBHm_Chr5g0019001 RchiOBHm_Chr5g0019071 RchiOBHm_Chr5g0028941 RchiOBHm_Chr5g0052141 RchiOBHm_Chr5g0054721 RchiOBHm_Chr5g0054801 RchiOBHm_Chr5g0054931 RchiOBHm_Chr5g0054941 RchiOBHm_Chr6g0259291 RchiOBHm_Chr6g0292991 RchiOBHm_Chr6g0306911
rosa_laevigata	RLG00000009465 RLG00000009468 RLG00000010755 RLG00000011976 RLG00000014541 RLG00000014542 RLG00000015169 RLG00000022308 RLG00000027529 RLG00000029324 RLG00000030244 RLG00000030245 RLG00000032210 RLG00000032211 RLG00000032257 RLG00000032259 RLG00000032260 RLG00000032261 RLG00000032262 RLG00000032268 RLG00000032269 RLG00000032272 RLG00000032282 RLG00000032283 RLG00000032308 RLG00000032413 RLG00000033129 RLG00000034945
rosa_multiflora	Rmu_co8241929.1_g000001 Rmu_co8269227.1_g000001 Rmu_co8461259.1_g000001 Rmu_co8495385.1_g000001 Rmu_sc0000028.1_g000003 Rmu_sc0000083.1_g000009 Rmu_sc0000083.1_g000010 Rmu_sc0000083.1_g000031 Rmu_sc0000083.1_g000043 Rmu_sc0000083.1_g000046 Rmu_sc0000083.1_g000047 Rmu_sc0000563.1_g000024 Rmu_sc0000842.1_g000012 Rmu_sc0001235.1_g000001 Rmu_sc0001235.1_g000005 Rmu_sc0001235.1_g000009 Rmu_sc0001235.1_g000015 Rmu_sc0001235.1_g000016 Rmu_sc0001235.1_g000017 Rmu_sc0001473.1_g000036 Rmu_sc0001607.1_g000016 Rmu_sc0001788.1_g000006 Rmu_sc0002018.1_g000008 Rmu_sc0002028.1_g000001 Rmu_sc0002680.1_g000011 Rmu_sc0002798.1_g000023 Rmu_sc0002886.1_g000023 Rmu_sc0002989.1_g000012 Rmu_sc0003064.1_g000009 Rmu_sc0003382.1_g000006 Rmu_sc0003630.1_g000002 Rmu_sc0003630.1_g000047 Rmu_sc0003630.1_g000066 Rmu_sc0003630.1_g000067 Rmu_sc0003649.1_g000010 Rmu_sc0003688.1_g000005 Rmu_sc0003920.1_g000007 Rmu_sc0004765.1_g000007 Rmu_sc0004765.1_g000008 Rmu_sc0004920.1_g000011 Rmu_sc0005223.1_g000008 Rmu_sc0005223.1_g000010 Rmu_sc0005223.1_g000015 Rmu_sc0005223.1_g000016 Rmu_sc0005319.1_g000014 Rmu_sc0005319.1_g000015 Rmu_sc0005319.1_g000018 Rmu_sc0005319.1_g000019 Rmu_sc0005319.1_g000025 Rmu_sc0005418.1_g000007 Rmu_sc0005418.1_g000012 Rmu_sc0006106.1_g000011 Rmu_sc0006914.1_g000012 Rmu_sc0007023.1_g000001 Rmu_sc0007662.1_g000001 Rmu_sc0007920.1_g000002 Rmu_sc0007920.1_g000013 Rmu_sc0008053.1_g000016 Rmu_sc0008053.1_g000017 Rmu_sc0008053.1_g000018 Rmu_sc0008053.1_g000023 Rmu_sc0008053.1_g000050 Rmu_sc0009042.1_g000007 Rmu_sc0009042.1_g000008 Rmu_sc0010642.1_g000008 Rmu_sc0011109.1_g000002 Rmu_sc0011314.1_g000006 Rmu_sc0011974.1_g000006 Rmu_sc0012179.1_g000009 Rmu_sc0015169.1_g000001 Rmu_sc0016368.1_g000002 Rmu_sc0022659.1_g000001 Rmu_sc0027371.1_g000001 Rmu_sc0028213.1_g000001 Rmu_sc0034526.1_g000001 Rmu_sc0039366.1_g000002 Rmu_ssc0000050.1_g000084 Rmu_ssc0000050.1_g000088 Rmu_ssc0000167.1_g000017
rosa_roxburghii	Rroxscaffold_1G00011340 Rroxscaffold_1G00025710 Rroxscaffold_1G00025870 Rroxscaffold_1G00026070 Rroxscaffold_1G00026170 Rroxscaffold_1G00026200 Rroxscaffold_1G00026270 Rroxscaffold_1G00050950 Rroxscaffold_1G00058900 Rroxscaffold_1G00060040 Rroxscaffold_1G00060060 Rroxscaffold_1G00060070 Rroxscaffold_1G00060080 Rroxscaffold_1G00060110 Rroxscaffold_1G00060120 Rroxscaffold_1G00060140 Rroxscaffold_1G00060170 Rroxscaffold_1G00060210 Rroxscaffold_1G00060220 Rroxscaffold_1G00060240 Rroxscaffold_1G00060250 Rroxscaffold_1G00060260 Rroxscaffold_1G00060300 Rroxscaffold_1G00060320 Rroxscaffold_1G00060330 Rroxscaffold_1G00060900 Rroxscaffold_1G00060920 Rroxscaffold_1G00073980 Rroxscaffold_2G00077560 Rroxscaffold_2G00077580 Rroxscaffold_2G00146990 Rroxscaffold_3G00236200 Rroxscaffold_3G00236210 Rroxscaffold_3G00236220 Rroxscaffold_4G00292760 Rroxscaffold_4G00315200 Rroxscaffold_4G00315240 Rroxscaffold_4G00326240 Rroxscaffold_4G00328980 Rroxscaffold_5G00341890 Rroxscaffold_7G00161350 Rroxscaffold_7G00174490 Rroxscaffold_7G00206550
rosa_rugosa	Rorug01G0093000 Rorug01G0093100 Rorug01G0128800 Rorug01G0312000 Rorug01G0312000 Rorug02G0581100 Rorug03G0224600 Rorug05G0028500 Rorug05G0032700 Rorug05G0032800 Rorug05G0032900 Rorug05G0032900 Rorug05G0033000 Rorug05G0033100 Rorug05G0033300 Rorug05G0033400 Rorug05G0033900 Rorug05G0034900 Rorug05G0035000 Rorug05G0046900 Rorug05G0047500 Rorug05G0047500 Rorug05G0111600 Rorug05G0287900 Rorug05G0288700 Rorug05G0586900 Rorug06G0231700 Rorug06G0354300 Rorug06G0354400
rosa_samantha	Rh1DG122200 Rh2DG686800 Rh5BG136600 Rh6AG344500 Rh6BG351900 Rh6CG359000 Rh6CG359100 Rh6CG387600 Rh6CG480500 Rh6DG344600 Rh6DG467400
rosa_wichuraiana	Rw0G010250 Rw0G014820 Rw0G021400 Rw0G023100 Rw1G009450 Rw1G012100 Rw1G012180 Rw1G028360 Rw2G054260 Rw3G024370 Rw3G024420 Rw3G024470 Rw5G010850 Rw5G011010 Rw5G011020 Rw5G011050 Rw5G011080 Rw5G011120 Rw5G012300 Rw5G018470 Rw5G033830 Rw6G008930 Rw6G030070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	849
AccB1I	GGYRCC	1 cut(s)	1463
AccI	GTMKAC	2 cut(s)	501, 1063
AciI	CCGC	7 cut(s)	246, 351, 741, 955, 1131, 1917, 2203
AclWI	GGATC	7 cut(s)	267, 890, 903, 1234, 1247, 1945, 2085
AcoI	YGGCCR	4 cut(s)	247, 1255, 1841, 2200
AcsI	RAATTY	1 cut(s)	337
AcuI	CTGAAG	6 cut(s)	129, 613, 1106, 1446, 1547, 1964
AcyI	GRCGYC	1 cut(s)	846
AfaI	GTAC	7 cut(s)	55, 128, 859, 871, 1271, 1984, 2084
AfiI	CCNNNNNNNGG	2 cut(s)	779, 1249
AflII	CTTAAG	1 cut(s)	211
AflIII	ACRYGT	1 cut(s)	205
AgsI	TTSAA	5 cut(s)	224, 611, 625, 1668, 2195
AhlI	ACTAGT	1 cut(s)	863
AjnI	CCWGG	3 cut(s)	82, 1242, 1953
AjuI	GAANNNNNNNTTGG	2 cut(s)	2091, 2123
AloI	GAACNNNNNNTCC	1 cut(s)	37
Alw21I	GWGCWC	2 cut(s)	1365, 1698
Alw26I	GTCTC	3 cut(s)	764, 1320, 1415
AlwI	GGATC	7 cut(s)	267, 890, 903, 1234, 1247, 1945, 2085
AlwNI	CAGNNNCTG	1 cut(s)	464
AoxI	GGCC	7 cut(s)	200, 247, 1163, 1255, 1841, 2006, 2200
ApeKI	GCWGC	8 cut(s)	58, 492, 978, 1410, 1961, 1973, 2027, 2148
ApoI	RAATTY	1 cut(s)	337
Asp700I	GAANNNNTTC	1 cut(s)	645
AspS9I	GGNCC	1 cut(s)	1163
AsuC2I	CCSGG	1 cut(s)	162
AsuHPI	GGTGA	5 cut(s)	76, 98, 175, 1447, 1876
AsuII	TTCGAA	1 cut(s)	534
AxyI	CCTNAGG	1 cut(s)	2088
BalI	TGGCCA	1 cut(s)	1257
BamHI	GGATCC	2 cut(s)	895, 1239
BanI	GGYRCC	1 cut(s)	1463
BanII	GRGCYC	1 cut(s)	1698
BauI	CACGAG	1 cut(s)	1364
Bbv12I	GWGCWC	2 cut(s)	1365, 1698
BbvI	GCAGC	8 cut(s)	70, 479, 965, 1397, 1948, 1960, 2014, 2160
BccI	CCATC	5 cut(s)	461, 806, 1034, 1193, 1602
BceAI	ACGGC	2 cut(s)	1150, 1856
BcgI	CGANNNNNNTGC	6 cut(s)	491, 525, 742, 776, 1453, 1487
BciT130I	CCWGG	3 cut(s)	84, 1244, 1955
BcnI	CCSGG	1 cut(s)	162
BcoDI	GTCTC	3 cut(s)	764, 1320, 1415
BcuI	ACTAGT	1 cut(s)	863
BfaI	CTAG	7 cut(s)	686, 774, 864, 1418, 1721, 1730, 2036
BfmI	CTRYAG	6 cut(s)	369, 493, 636, 790, 907, 1974
BfrI	CTTAAG	1 cut(s)	211
BglII	AGATCT	1 cut(s)	643
BmcAI	AGTACT	1 cut(s)	871
Bme1390I	CCNGG	4 cut(s)	84, 162, 1244, 1955
BmgT120I	GGNCC	1 cut(s)	1163
BmiI	GGNNCC	5 cut(s)	674, 897, 1241, 1465, 1489
BmrFI	CCNGG	4 cut(s)	84, 162, 1244, 1955
BmsI	GCATC	3 cut(s)	561, 785, 2175
BoxI	GACNNNNGTC	1 cut(s)	582
Bpu14I	TTCGAA	1 cut(s)	534
BpuEI	CTTGAG	1 cut(s)	2065
BpuMI	CCSGG	1 cut(s)	162
Bsa29I	ATCGAT	1 cut(s)	271
BsaBI	GATNNNNATC	1 cut(s)	816
BsaHI	GRCGYC	1 cut(s)	846
BsaJI	CCNNGG	3 cut(s)	1243, 1258, 2009
BsaXI	ACNNNNNCTCC	2 cut(s)	5, 35
Bsc4I	CCNNNNNNNGG	2 cut(s)	779, 1249
Bse1I	ACTGG	3 cut(s)	336, 2142, 2203
Bse21I	CCTNAGG	1 cut(s)	2088
Bse3DI	GCAATG	1 cut(s)	805
Bse8I	GATNNNNATC	1 cut(s)	816
BseBI	CCWGG	3 cut(s)	84, 1244, 1955
BseCI	ATCGAT	1 cut(s)	271
BseDI	CCNNGG	3 cut(s)	1243, 1258, 2009
BseGI	GGATG	5 cut(s)	1204, 1590, 1633, 1909, 2019
BseJI	GATNNNNATC	1 cut(s)	816
BseLI	CCNNNNNNNGG	2 cut(s)	779, 1249
BseMI	GCAATG	1 cut(s)	805
BseMII	CTCAG	6 cut(s)	300, 1167, 1185, 1221, 1827, 2079
BseNI	ACTGG	3 cut(s)	336, 2142, 2203
BseRI	GAGGAG	3 cut(s)	326, 1561, 2105
BseXI	GCAGC	8 cut(s)	70, 479, 965, 1397, 1948, 1960, 2014, 2160
BsgI	GTGCAG	1 cut(s)	29
BshFI	GGCC	7 cut(s)	202, 249, 1165, 1257, 1843, 2008, 2202
BshNI	GGYRCC	1 cut(s)	1463
BshVI	ATCGAT	1 cut(s)	271
BsiHKAI	GWGCWC	2 cut(s)	1365, 1698
BsiSI	CCGG	2 cut(s)	162, 1286
BslI	CCNNNNNNNGG	2 cut(s)	779, 1249
BsmAI	GTCTC	3 cut(s)	764, 1320, 1415
BsmI	GAATGC	1 cut(s)	417
BsnI	GGCC	7 cut(s)	202, 249, 1165, 1257, 1843, 2008, 2202
Bsp119I	TTCGAA	1 cut(s)	534
Bsp1286I	GDGCHC	2 cut(s)	1365, 1698
Bsp1407I	TGTACA	2 cut(s)	1269, 1982
Bsp143I	GATC	7 cut(s)	272, 643, 895, 1239, 1474, 1950, 2077
BspACI	CCGC	7 cut(s)	246, 351, 741, 955, 1131, 1917, 2203
BspANI	GGCC	7 cut(s)	202, 249, 1165, 1257, 1843, 2008, 2202
BspCNI	CTCAG	6 cut(s)	301, 1166, 1186, 1222, 1826, 2080
BspDI	ATCGAT	1 cut(s)	271
BspHI	TCATGA	1 cut(s)	666
BspLI	GGNNCC	5 cut(s)	674, 897, 1241, 1465, 1489
BspMAI	CTGCAG	4 cut(s)	373, 497, 794, 1978
BspPI	GGATC	7 cut(s)	267, 890, 903, 1234, 1247, 1945, 2085
BspQI	GCTCTTC	1 cut(s)	1686
BspT104I	TTCGAA	1 cut(s)	534
BspT107I	GGYRCC	1 cut(s)	1463
BspTI	CTTAAG	1 cut(s)	211
BsrDI	GCAATG	1 cut(s)	805
BsrGI	TGTACA	2 cut(s)	1269, 1982
BsrI	ACTGG	3 cut(s)	336, 2142, 2203
BssECI	CCNNGG	3 cut(s)	1243, 1258, 2009
BssMI	GATC	7 cut(s)	272, 643, 895, 1239, 1474, 1950, 2077
BssNAI	GTATAC	1 cut(s)	1064
BssNI	GRCGYC	1 cut(s)	846
BssSI	CACGAG	1 cut(s)	1364
BssT1I	CCWWGG	2 cut(s)	1258, 2009
Bst1107I	GTATAC	1 cut(s)	1064
Bst2BI	CACGAG	1 cut(s)	1364
Bst2UI	CCWGG	3 cut(s)	84, 1244, 1955
Bst4CI	ACNGT	6 cut(s)	320, 890, 1267, 1331, 1523, 1848
Bst6I	CTCTTC	1 cut(s)	1686
BstACI	GRCGYC	1 cut(s)	846
BstAFI	CTTAAG	1 cut(s)	211
BstAPI	GCANNNNNTGC	1 cut(s)	1970
BstAUI	TGTACA	2 cut(s)	1269, 1982
BstBI	TTCGAA	1 cut(s)	534
BstC8I	GCNNGC	4 cut(s)	251, 433, 716, 983
BstDEI	CTNAG	7 cut(s)	309, 958, 1153, 1194, 1230, 1813, 2088
BstEII	GGTNACC	2 cut(s)	950, 1724
BstENI	CCTNNNNNAGG	1 cut(s)	1247
BstF5I	GGATG	5 cut(s)	1204, 1590, 1633, 1909, 2019
BstKTI	GATC	7 cut(s)	275, 646, 898, 1242, 1477, 1953, 2080
BstMAI	GTCTC	3 cut(s)	764, 1320, 1415
BstMBI	GATC	7 cut(s)	272, 643, 895, 1239, 1474, 1950, 2077
BstMWI	GCNNNNNNNGC	5 cut(s)	804, 1649, 1970, 2154, 2163
BstNI	CCWGG	3 cut(s)	84, 1244, 1955
BstNSI	RCATGY	4 cut(s)	209, 435, 714, 1138
BstPAI	GACNNNNGTC	1 cut(s)	582
BstPI	GGTNACC	2 cut(s)	950, 1724
BstSCI	CCNGG	4 cut(s)	82, 160, 1242, 1953
BstSFI	CTRYAG	6 cut(s)	369, 493, 636, 790, 907, 1974
BstV1I	GCAGC	8 cut(s)	70, 479, 965, 1397, 1948, 1960, 2014, 2160
BstX2I	RGATCY	4 cut(s)	643, 895, 1239, 1950
BstYI	RGATCY	4 cut(s)	643, 895, 1239, 1950
BstZ17I	GTATAC	1 cut(s)	1064
Bsu15I	ATCGAT	1 cut(s)	271
Bsu36I	CCTNAGG	1 cut(s)	2088
BsuRI	GGCC	7 cut(s)	202, 249, 1165, 1257, 1843, 2008, 2202
BsuTUI	ATCGAT	1 cut(s)	271
BtgZI	GCGATG	1 cut(s)	1788
BtsCI	GGATG	5 cut(s)	1204, 1590, 1633, 1909, 2019
BtsIMutI	CAGTG	2 cut(s)	329, 1272
Cac8I	GCNNGC	4 cut(s)	251, 433, 716, 983
CaiI	CAGNNNCTG	1 cut(s)	464
CciI	TCATGA	1 cut(s)	666
Cfr13I	GGNCC	1 cut(s)	1163
ClaI	ATCGAT	1 cut(s)	271
CsiI	ACCWGGT	1 cut(s)	82
Csp6I	GTAC	7 cut(s)	54, 127, 858, 870, 1270, 1983, 2083
CviQI	GTAC	7 cut(s)	54, 127, 858, 870, 1270, 1983, 2083
DdeI	CTNAG	7 cut(s)	309, 958, 1153, 1194, 1230, 1813, 2088
DpnI	GATC	7 cut(s)	274, 645, 897, 1241, 1476, 1952, 2079
DpnII	GATC	7 cut(s)	272, 643, 895, 1239, 1474, 1950, 2077
DraI	TTTAAA	1 cut(s)	1837
EaeI	YGGCCR	4 cut(s)	247, 1255, 1841, 2200
Eam1104I	CTCTTC	1 cut(s)	1686
EarI	CTCTTC	1 cut(s)	1686
EciI	GGCGGA	1 cut(s)	340
Ecl136II	GAGCTC	1 cut(s)	1696
Eco130I	CCWWGG	2 cut(s)	1258, 2009
Eco24I	GRGCYC	1 cut(s)	1698
Eco32I	GATATC	1 cut(s)	1603
Eco53kI	GAGCTC	1 cut(s)	1696
Eco57I	CTGAAG	6 cut(s)	129, 613, 1106, 1446, 1547, 1964
Eco81I	CCTNAGG	1 cut(s)	2088
Eco91I	GGTNACC	2 cut(s)	950, 1724
EcoICRI	GAGCTC	1 cut(s)	1696
EcoNI	CCTNNNNNAGG	1 cut(s)	1247
EcoO65I	GGTNACC	2 cut(s)	950, 1724
EcoRII	CCWGG	3 cut(s)	82, 1242, 1953
EcoRV	GATATC	1 cut(s)	1603
EcoT14I	CCWWGG	2 cut(s)	1258, 2009
EcoT38I	GRGCYC	1 cut(s)	1698
ErhI	CCWWGG	2 cut(s)	1258, 2009
FalI	AAGNNNNNCTT	6 cut(s)	249, 281, 611, 643, 1305, 1337
FauNDI	CATATG	1 cut(s)	737
FblI	GTMKAC	2 cut(s)	501, 1063
FokI	GGATG	5 cut(s)	1211, 1577, 1640, 1916, 2026
FriOI	GRGCYC	1 cut(s)	1698
FspBI	CTAG	7 cut(s)	686, 774, 864, 1418, 1721, 1730, 2036
HaeIII	GGCC	7 cut(s)	202, 249, 1165, 1257, 1843, 2008, 2202
HapII	CCGG	2 cut(s)	162, 1286
Hin1I	GRCGYC	1 cut(s)	846
HincII	GTYRAC	3 cut(s)	366, 502, 1140
HindII	GTYRAC	3 cut(s)	366, 502, 1140
HinfI	GANTC	7 cut(s)	449, 498, 652, 764, 817, 1149, 1499
HpaI	GTTAAC	1 cut(s)	366
HpaII	CCGG	2 cut(s)	162, 1286
HphI	GGTGA	5 cut(s)	76, 98, 175, 1447, 1876
Hpy166II	GTNNAC	9 cut(s)	54, 366, 502, 858, 1064, 1140, 1270, 1633, 1983
Hpy188I	TCNGA	7 cut(s)	651, 708, 895, 1103, 1527, 1944, 2077
Hpy188III	TCNNGA	9 cut(s)	398, 575, 667, 761, 1155, 1418, 1598, 1625, 2170
Hpy8I	GTNNAC	9 cut(s)	54, 366, 502, 858, 1064, 1140, 1270, 1633, 1983
Hpy99I	CGWCG	2 cut(s)	293, 506
HpyAV	CCTTC	4 cut(s)	61, 797, 1081, 1939
HpyCH4III	ACNGT	6 cut(s)	320, 890, 1267, 1331, 1523, 1848
HpyCH4IV	ACGT	3 cut(s)	504, 846, 1392
HpyF10VI	GCNNNNNNNGC	5 cut(s)	804, 1649, 1970, 2154, 2163
HpyF3I	CTNAG	7 cut(s)	309, 958, 1153, 1194, 1230, 1813, 2088
HpySE526I	ACGT	3 cut(s)	504, 846, 1392
Hsp92I	GRCGYC	1 cut(s)	846
KspAI	GTTAAC	1 cut(s)	366
Kzo9I	GATC	7 cut(s)	272, 643, 895, 1239, 1474, 1950, 2077
LguI	GCTCTTC	1 cut(s)	1686
LmnI	GCTCC	2 cut(s)	1104, 1157
Lsp1109I	GCAGC	8 cut(s)	70, 479, 965, 1397, 1948, 1960, 2014, 2160
LweI	GCATC	3 cut(s)	561, 785, 2175
MabI	ACCWGGT	1 cut(s)	82
MaeI	CTAG	7 cut(s)	686, 774, 864, 1418, 1721, 1730, 2036
MaeII	ACGT	3 cut(s)	504, 846, 1392
MaeIII	GTNAC	8 cut(s)	152, 380, 681, 950, 1018, 1724, 1868, 2114
MalI	GATC	7 cut(s)	274, 645, 897, 1241, 1476, 1952, 2079
MboI	GATC	7 cut(s)	272, 643, 895, 1239, 1474, 1950, 2077
MflI	RGATCY	4 cut(s)	643, 895, 1239, 1950
MhlI	GDGCHC	2 cut(s)	1365, 1698
MlsI	TGGCCA	1 cut(s)	1257
MluCI	AATT	5 cut(s)	91, 337, 1008, 1109, 1968
MluNI	TGGCCA	1 cut(s)	1257
MlyI	GAGTC	2 cut(s)	507, 1143
MmeI	TCCRAC	6 cut(s)	498, 679, 703, 901, 1881, 2055
Mox20I	TGGCCA	1 cut(s)	1257
MroXI	GAANNNNTTC	1 cut(s)	645
MscI	TGGCCA	1 cut(s)	1257
MseI	TTAA	7 cut(s)	212, 237, 267, 365, 1637, 1760, 1836
MslI	CAYNNNNRTG	3 cut(s)	224, 971, 1482
Msp20I	TGGCCA	1 cut(s)	1257
MspA1I	CMGCKG	4 cut(s)	61, 246, 833, 2205
MspCI	CTTAAG	1 cut(s)	211
MspI	CCGG	2 cut(s)	162, 1286
MspR9I	CCNGG	4 cut(s)	84, 162, 1244, 1955
Mva1269I	GAATGC	1 cut(s)	417
MvaI	CCWGG	3 cut(s)	84, 1244, 1955
MwoI	GCNNNNNNNGC	5 cut(s)	804, 1649, 1970, 2154, 2163
NciI	CCSGG	1 cut(s)	162
NdeI	CATATG	1 cut(s)	737
NdeII	GATC	7 cut(s)	272, 643, 895, 1239, 1474, 1950, 2077
NlaIV	GGNNCC	5 cut(s)	674, 897, 1241, 1465, 1489
NmuCI	GTSAC	1 cut(s)	2114
NspI	RCATGY	4 cut(s)	209, 435, 714, 1138
NspV	TTCGAA	1 cut(s)	534
PaeI	GCATGC	1 cut(s)	435
PagI	TCATGA	1 cut(s)	666
PciI	ACATGT	1 cut(s)	205
PciSI	GCTCTTC	1 cut(s)	1686
PcsI	WCGNNNNNNNCGW	2 cut(s)	603, 1770
PctI	GAATGC	1 cut(s)	417
PdmI	GAANNNNTTC	1 cut(s)	645
PfeI	GAWTC	5 cut(s)	449, 652, 764, 817, 1499
PfoI	TCCNGGA	2 cut(s)	160, 1953
PleI	GAGTC	2 cut(s)	506, 1143
PpsI	GAGTC	2 cut(s)	506, 1143
PscI	ACATGT	1 cut(s)	205
PshAI	GACNNNNGTC	1 cut(s)	582
Psp124BI	GAGCTC	1 cut(s)	1698
Psp6I	CCWGG	3 cut(s)	82, 1242, 1953
PspEI	GGTNACC	2 cut(s)	950, 1724
PspGI	CCWGG	3 cut(s)	82, 1242, 1953
PspN4I	GGNNCC	5 cut(s)	674, 897, 1241, 1465, 1489
PspPI	GGNCC	1 cut(s)	1163
PstI	CTGCAG	4 cut(s)	373, 497, 794, 1978
PstNI	CAGNNNCTG	1 cut(s)	464
PsuI	RGATCY	4 cut(s)	643, 895, 1239, 1950
PvuII	CAGCTG	2 cut(s)	61, 833
RsaI	GTAC	7 cut(s)	55, 128, 859, 871, 1271, 1984, 2084
RsaNI	GTAC	7 cut(s)	54, 127, 858, 870, 1270, 1983, 2083
RseI	CAYNNNNRTG	3 cut(s)	224, 971, 1482
SacI	GAGCTC	1 cut(s)	1698
SalI	GTCGAC	1 cut(s)	500
SapI	GCTCTTC	1 cut(s)	1686
SaqAI	TTAA	7 cut(s)	212, 237, 267, 365, 1637, 1760, 1836
Sau3AI	GATC	7 cut(s)	272, 643, 895, 1239, 1474, 1950, 2077
Sau96I	GGNCC	1 cut(s)	1163
ScaI	AGTACT	1 cut(s)	871
SchI	GAGTC	2 cut(s)	507, 1143
ScrFI	CCNGG	4 cut(s)	84, 162, 1244, 1955
SduI	GDGCHC	2 cut(s)	1365, 1698
SexAI	ACCWGGT	1 cut(s)	82
SfaNI	GCATC	3 cut(s)	561, 785, 2175
SfcI	CTRYAG	6 cut(s)	369, 493, 636, 790, 907, 1974
SfuI	TTCGAA	1 cut(s)	534
SmiMI	CAYNNNNRTG	3 cut(s)	224, 971, 1482
SmlI	CTYRAG	2 cut(s)	211, 2044
SmoI	CTYRAG	2 cut(s)	211, 2044
SpeI	ACTAGT	1 cut(s)	863
SphI	GCATGC	1 cut(s)	435
Sse9I	AATT	5 cut(s)	91, 337, 1008, 1109, 1968
SsiI	CCGC	7 cut(s)	246, 351, 741, 955, 1131, 1917, 2203
SspI	AATATT	3 cut(s)	1618, 1742, 2184
SspMI	CTAG	7 cut(s)	686, 774, 864, 1418, 1721, 1730, 2036
SstI	GAGCTC	1 cut(s)	1698
StyD4I	CCNGG	4 cut(s)	82, 160, 1242, 1953
StyI	CCWWGG	2 cut(s)	1258, 2009
TaaI	ACNGT	6 cut(s)	320, 890, 1267, 1331, 1523, 1848
TaiI	ACGT	3 cut(s)	507, 849, 1395
TaqI	TCGA	8 cut(s)	271, 501, 534, 762, 1116, 1473, 1698, 2052
TasI	AATT	5 cut(s)	91, 337, 1008, 1109, 1968
TatI	WGTACW	4 cut(s)	857, 869, 1269, 1982
TauI	GCSGC	4 cut(s)	249, 1134, 1920, 2205
TfiI	GAWTC	5 cut(s)	449, 652, 764, 817, 1499
Tru1I	TTAA	7 cut(s)	212, 237, 267, 365, 1637, 1760, 1836
Tru9I	TTAA	7 cut(s)	212, 237, 267, 365, 1637, 1760, 1836
TscAI	CASTG	2 cut(s)	336, 1272
TseFI	GTSAC	1 cut(s)	2114
TseI	GCWGC	8 cut(s)	58, 492, 978, 1410, 1961, 1973, 2027, 2148
Tsp45I	GTSAC	1 cut(s)	2114
TspRI	CASTG	2 cut(s)	336, 1272
Vha464I	CTTAAG	1 cut(s)	211
XagI	CCTNNNNNAGG	1 cut(s)	1247
XapI	RAATTY	1 cut(s)	337
XbaI	TCTAGA	1 cut(s)	1417
XceI	RCATGY	4 cut(s)	209, 435, 714, 1138
XcmI	CCANNNNNNNNNTGG	2 cut(s)	104, 1033
XmiI	GTMKAC	2 cut(s)	501, 1063
XmnI	GAANNNNTTC	1 cut(s)	645
XspI	CTAG	7 cut(s)	686, 774, 864, 1418, 1721, 1730, 2036
ZraI	GACGTC	1 cut(s)	847
ZrmI	AGTACT	1 cut(s)	871

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.