Wall-associated receptor kinase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 822 |
| AccB1I | GGYRCC | 1 cut(s) | 1202 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 365 |
| AccII | CGCG | 1 cut(s) | 712 |
| AciI | CCGC | 3 cut(s) | 171, 845, 1172 |
| AclWI | GGATC | 5 cut(s) | 217, 537, 550, 770, 783 |
| AcoI | YGGCCR | 1 cut(s) | 684 |
| AcsI | RAATTY | 1 cut(s) | 951 |
| AcuI | CTGAAG | 1 cut(s) | 233 |
| AcyI | GRCGYC | 1 cut(s) | 819 |
| AfaI | GTAC | 4 cut(s) | 58, 155, 494, 1148 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 365 |
| AgsI | TTSAA | 9 cut(s) | 16, 32, 50, 529, 571, 755, 795, 927, 950 |
| AhlI | ACTAGT | 1 cut(s) | 133 |
| AjiI | CACGTC | 1 cut(s) | 646 |
| AjnI | CCWGG | 1 cut(s) | 881 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 650, 682 |
| AluBI | AGCT | 3 cut(s) | 707, 850, 912 |
| AluI | AGCT | 3 cut(s) | 707, 850, 912 |
| Alw26I | GTCTC | 6 cut(s) | 68, 212, 458, 636, 983, 1133 |
| AlwI | GGATC | 5 cut(s) | 217, 537, 550, 770, 783 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 587 |
| Ama87I | CYCGRG | 1 cut(s) | 1154 |
| AoxI | GGCC | 3 cut(s) | 684, 884, 1049 |
| ApeKI | GCWGC | 1 cut(s) | 305 |
| ApoI | RAATTY | 1 cut(s) | 951 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 36, 786 |
| AspLEI | GCGC | 2 cut(s) | 371, 596 |
| AsuHPI | GGTGA | 2 cut(s) | 302, 1152 |
| AvaI | CYCGRG | 1 cut(s) | 1154 |
| BamHI | GGATCC | 2 cut(s) | 542, 775 |
| BanI | GGYRCC | 1 cut(s) | 1202 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 462, 494 |
| BbsI | GAAGAC | 1 cut(s) | 136 |
| BbvI | GCAGC | 1 cut(s) | 317 |
| BccI | CCATC | 1 cut(s) | 1190 |
| BceAI | ACGGC | 1 cut(s) | 699 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 496, 530 |
| BciT130I | CCWGG | 1 cut(s) | 883 |
| BciVI | GTATCC | 1 cut(s) | 761 |
| BclI | TGATCA | 1 cut(s) | 385 |
| BcoDI | GTCTC | 6 cut(s) | 68, 212, 458, 636, 983, 1133 |
| BcuI | ACTAGT | 1 cut(s) | 133 |
| BfaI | CTAG | 6 cut(s) | 134, 198, 599, 725, 842, 905 |
| BfmI | CTRYAG | 3 cut(s) | 237, 588, 823 |
| BfoI | RGCGCY | 1 cut(s) | 372 |
| BfuI | GTATCC | 1 cut(s) | 761 |
| BisI | GCNGC | 3 cut(s) | 172, 306, 1173 |
| BlsI | GCNGC | 3 cut(s) | 173, 307, 1174 |
| BmcAI | AGTACT | 1 cut(s) | 155 |
| Bme1390I | CCNGG | 1 cut(s) | 883 |
| BmeT110I | CYCGRG | 1 cut(s) | 1154 |
| BmgBI | CACGTC | 1 cut(s) | 646 |
| BmiI | GGNNCC | 3 cut(s) | 544, 777, 1204 |
| BmrFI | CCNGG | 1 cut(s) | 883 |
| BmrI | ACTGGG | 2 cut(s) | 1152, 1239 |
| BmsI | GCATC | 5 cut(s) | 587, 890, 949, 993, 1114 |
| BmuI | ACTGGG | 2 cut(s) | 1152, 1239 |
| BpiI | GAAGAC | 1 cut(s) | 136 |
| BsaHI | GRCGYC | 1 cut(s) | 819 |
| BsaI | GGTCTC | 2 cut(s) | 68, 458 |
| BsaJI | CCNNGG | 3 cut(s) | 286, 1071, 1109 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 198, 228, 610, 640, 1130, 1160 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 365 |
| Bse118I | RCCGGY | 1 cut(s) | 1205 |
| Bse1I | ACTGG | 4 cut(s) | 486, 753, 1147, 1234 |
| BseBI | CCWGG | 1 cut(s) | 883 |
| BseDI | CCNNGG | 3 cut(s) | 286, 1071, 1109 |
| BseGI | GGATG | 3 cut(s) | 676, 1092, 1105 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 365 |
| BseMII | CTCAG | 2 cut(s) | 79, 431 |
| BseNI | ACTGG | 4 cut(s) | 486, 753, 1147, 1234 |
| BseRI | GAGGAG | 2 cut(s) | 992, 1151 |
| BseXI | GCAGC | 1 cut(s) | 317 |
| Bsh1236I | CGCG | 1 cut(s) | 712 |
| BshFI | GGCC | 3 cut(s) | 686, 886, 1051 |
| BshNI | GGYRCC | 1 cut(s) | 1202 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1154 |
| BsiSI | CCGG | 2 cut(s) | 1128, 1206 |
| BslFI | GGGAC | 1 cut(s) | 772 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 365 |
| BsmAI | GTCTC | 6 cut(s) | 68, 212, 458, 636, 983, 1133 |
| BsmBI | CGTCTC | 2 cut(s) | 636, 983 |
| BsmFI | GGGAC | 1 cut(s) | 772 |
| BsmI | GAATGC | 1 cut(s) | 944 |
| BsnI | GGCC | 3 cut(s) | 686, 886, 1051 |
| Bso31I | GGTCTC | 2 cut(s) | 68, 458 |
| BsoBI | CYCGRG | 1 cut(s) | 1154 |
| Bsp1407I | TGTACA | 1 cut(s) | 56 |
| Bsp143I | GATC | 5 cut(s) | 209, 385, 542, 727, 775 |
| Bsp19I | CCATGG | 2 cut(s) | 1071, 1109 |
| BspACI | CCGC | 3 cut(s) | 171, 845, 1172 |
| BspANI | GGCC | 3 cut(s) | 686, 886, 1051 |
| BspCNI | CTCAG | 2 cut(s) | 78, 430 |
| BspFNI | CGCG | 1 cut(s) | 712 |
| BspLI | GGNNCC | 3 cut(s) | 544, 777, 1204 |
| BspMAI | CTGCAG | 2 cut(s) | 241, 592 |
| BspPI | GGATC | 5 cut(s) | 217, 537, 550, 770, 783 |
| BspT107I | GGYRCC | 1 cut(s) | 1202 |
| BspTNI | GGTCTC | 2 cut(s) | 68, 458 |
| BsrFI | RCCGGY | 1 cut(s) | 1205 |
| BsrGI | TGTACA | 1 cut(s) | 56 |
| BsrI | ACTGG | 4 cut(s) | 486, 753, 1147, 1234 |
| BssAI | RCCGGY | 1 cut(s) | 1205 |
| BssECI | CCNNGG | 3 cut(s) | 286, 1071, 1109 |
| BssMI | GATC | 5 cut(s) | 209, 385, 542, 727, 775 |
| BssNI | GRCGYC | 1 cut(s) | 819 |
| BssT1I | CCWWGG | 3 cut(s) | 286, 1071, 1109 |
| Bst2UI | CCWGG | 1 cut(s) | 883 |
| Bst4CI | ACNGT | 1 cut(s) | 994 |
| BstACI | GRCGYC | 1 cut(s) | 819 |
| BstAUI | TGTACA | 1 cut(s) | 56 |
| BstC8I | GCNNGC | 3 cut(s) | 592, 910, 1244 |
| BstDEI | CTNAG | 2 cut(s) | 65, 417 |
| BstDSI | CCRYGG | 2 cut(s) | 1071, 1109 |
| BstEII | GGTNACC | 1 cut(s) | 1158 |
| BstF5I | GGATG | 3 cut(s) | 676, 1092, 1105 |
| BstFNI | CGCG | 1 cut(s) | 712 |
| BstH2I | RGCGCY | 1 cut(s) | 372 |
| BstHHI | GCGC | 2 cut(s) | 371, 596 |
| BstKTI | GATC | 5 cut(s) | 212, 388, 545, 730, 778 |
| BstMAI | GTCTC | 6 cut(s) | 68, 212, 458, 636, 983, 1133 |
| BstMBI | GATC | 5 cut(s) | 209, 385, 542, 727, 775 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 909, 1013, 1128 |
| BstNI | CCWGG | 1 cut(s) | 883 |
| BstNSI | RCATGY | 1 cut(s) | 1246 |
| BstPI | GGTNACC | 1 cut(s) | 1158 |
| BstSCI | CCNGG | 1 cut(s) | 881 |
| BstSFI | CTRYAG | 3 cut(s) | 237, 588, 823 |
| BstUI | CGCG | 1 cut(s) | 712 |
| BstV1I | GCAGC | 1 cut(s) | 317 |
| BstV2I | GAAGAC | 1 cut(s) | 136 |
| BstX2I | RGATCY | 3 cut(s) | 209, 542, 775 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 167 |
| BstYI | RGATCY | 3 cut(s) | 209, 542, 775 |
| BsuI | GTATCC | 1 cut(s) | 761 |
| BsuRI | GGCC | 3 cut(s) | 686, 886, 1051 |
| BtgI | CCRYGG | 2 cut(s) | 1071, 1109 |
| BtrI | CACGTC | 1 cut(s) | 646 |
| BtsCI | GGATG | 3 cut(s) | 676, 1092, 1105 |
| BtsIMutI | CAGTG | 1 cut(s) | 746 |
| Cac8I | GCNNGC | 3 cut(s) | 592, 910, 1244 |
| CaiI | CAGNNNCTG | 1 cut(s) | 587 |
| CfoI | GCGC | 2 cut(s) | 371, 596 |
| Cfr10I | RCCGGY | 1 cut(s) | 1205 |
| CseI | GACGC | 1 cut(s) | 769 |
| Csp6I | GTAC | 4 cut(s) | 57, 154, 493, 1147 |
| CviAII | CATG | 8 cut(s) | 203, 272, 329, 503, 555, 1072, 1110, 1243 |
| CviQI | GTAC | 4 cut(s) | 57, 154, 493, 1147 |
| DdeI | CTNAG | 2 cut(s) | 65, 417 |
| DpnI | GATC | 5 cut(s) | 211, 387, 544, 729, 777 |
| DpnII | GATC | 5 cut(s) | 209, 385, 542, 727, 775 |
| EaeI | YGGCCR | 1 cut(s) | 684 |
| Eco130I | CCWWGG | 3 cut(s) | 286, 1071, 1109 |
| Eco147I | AGGCCT | 2 cut(s) | 886, 1051 |
| Eco31I | GGTCTC | 2 cut(s) | 68, 458 |
| Eco57I | CTGAAG | 1 cut(s) | 233 |
| Eco88I | CYCGRG | 1 cut(s) | 1154 |
| Eco91I | GGTNACC | 1 cut(s) | 1158 |
| EcoO65I | GGTNACC | 1 cut(s) | 1158 |
| EcoRII | CCWGG | 1 cut(s) | 881 |
| EcoT14I | CCWWGG | 3 cut(s) | 286, 1071, 1109 |
| ErhI | CCWWGG | 3 cut(s) | 286, 1071, 1109 |
| Esp3I | CGTCTC | 2 cut(s) | 636, 983 |
| FaeI | CATG | 8 cut(s) | 206, 275, 332, 506, 558, 1075, 1113, 1246 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 747, 779 |
| FaqI | GGGAC | 1 cut(s) | 772 |
| FatI | CATG | 8 cut(s) | 202, 271, 328, 502, 554, 1071, 1109, 1242 |
| FbaI | TGATCA | 1 cut(s) | 385 |
| Fnu4HI | GCNGC | 3 cut(s) | 172, 306, 1173 |
| FokI | GGATG | 3 cut(s) | 683, 1092, 1099 |
| Fsp4HI | GCNGC | 3 cut(s) | 172, 306, 1173 |
| FspBI | CTAG | 6 cut(s) | 134, 198, 599, 725, 842, 905 |
| GlaI | GCGC | 2 cut(s) | 370, 595 |
| GluI | GCNGC | 3 cut(s) | 172, 306, 1173 |
| HaeII | RGCGCY | 1 cut(s) | 372 |
| HaeIII | GGCC | 3 cut(s) | 686, 886, 1051 |
| HapII | CCGG | 2 cut(s) | 1128, 1206 |
| HgaI | GACGC | 1 cut(s) | 769 |
| HhaI | GCGC | 2 cut(s) | 371, 596 |
| Hin1I | GRCGYC | 1 cut(s) | 819 |
| Hin1II | CATG | 8 cut(s) | 206, 275, 332, 506, 558, 1075, 1113, 1246 |
| Hin6I | GCGC | 2 cut(s) | 369, 594 |
| HinP1I | GCGC | 2 cut(s) | 369, 594 |
| HindIII | AAGCTT | 1 cut(s) | 910 |
| HinfI | GANTC | 3 cut(s) | 141, 946, 1009 |
| HpaII | CCGG | 2 cut(s) | 1128, 1206 |
| HphI | GGTGA | 2 cut(s) | 302, 1152 |
| Hpy166II | GTNNAC | 2 cut(s) | 105, 313 |
| Hpy188I | TCNGA | 5 cut(s) | 42, 252, 420, 583, 697 |
| Hpy188III | TCNNGA | 5 cut(s) | 463, 725, 779, 956, 988 |
| Hpy8I | GTNNAC | 2 cut(s) | 105, 313 |
| HpyAV | CCTTC | 2 cut(s) | 287, 1076 |
| HpyCH4III | ACNGT | 1 cut(s) | 994 |
| HpyCH4IV | ACGT | 2 cut(s) | 645, 819 |
| HpyCH4V | TGCA | 6 cut(s) | 239, 305, 590, 743, 869, 1246 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 909, 1013, 1128 |
| HpyF3I | CTNAG | 2 cut(s) | 65, 417 |
| HpySE526I | ACGT | 2 cut(s) | 645, 819 |
| Hsp92I | GRCGYC | 1 cut(s) | 819 |
| Hsp92II | CATG | 8 cut(s) | 206, 275, 332, 506, 558, 1075, 1113, 1246 |
| HspAI | GCGC | 2 cut(s) | 369, 594 |
| Ksp22I | TGATCA | 1 cut(s) | 385 |
| Kzo9I | GATC | 5 cut(s) | 209, 385, 542, 727, 775 |
| LmnI | GCTCC | 3 cut(s) | 376, 704, 847 |
| Lsp1109I | GCAGC | 1 cut(s) | 317 |
| LweI | GCATC | 5 cut(s) | 587, 890, 949, 993, 1114 |
| MaeI | CTAG | 6 cut(s) | 134, 198, 599, 725, 842, 905 |
| MaeII | ACGT | 2 cut(s) | 645, 819 |
| MaeIII | GTNAC | 3 cut(s) | 815, 1158, 1219 |
| MalI | GATC | 5 cut(s) | 211, 387, 544, 729, 777 |
| MboI | GATC | 5 cut(s) | 209, 385, 542, 727, 775 |
| MboII | GAAGA | 4 cut(s) | 70, 141, 583, 915 |
| MflI | RGATCY | 3 cut(s) | 209, 542, 775 |
| MlyI | GAGTC | 1 cut(s) | 135 |
| MmeI | TCCRAC | 1 cut(s) | 398 |
| MroXI | GAANNNNTTC | 2 cut(s) | 36, 786 |
| MseI | TTAA | 3 cut(s) | 95, 396, 1259 |
| MslI | CAYNNNNRTG | 3 cut(s) | 86, 276, 432 |
| MspA1I | CMGCKG | 1 cut(s) | 1172 |
| MspI | CCGG | 2 cut(s) | 1128, 1206 |
| MspR9I | CCNGG | 1 cut(s) | 883 |
| Mva1269I | GAATGC | 1 cut(s) | 944 |
| MvaI | CCWGG | 1 cut(s) | 883 |
| MvnI | CGCG | 1 cut(s) | 712 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 909, 1013, 1128 |
| NcoI | CCATGG | 2 cut(s) | 1071, 1109 |
| NdeII | GATC | 5 cut(s) | 209, 385, 542, 727, 775 |
| NlaIII | CATG | 8 cut(s) | 206, 275, 332, 506, 558, 1075, 1113, 1246 |
| NlaIV | GGNNCC | 3 cut(s) | 544, 777, 1204 |
| NmuCI | GTSAC | 2 cut(s) | 815, 1158 |
| NspI | RCATGY | 1 cut(s) | 1246 |
| PaeI | GCATGC | 1 cut(s) | 1246 |
| PceI | AGGCCT | 2 cut(s) | 886, 1051 |
| PctI | GAATGC | 1 cut(s) | 944 |
| PdmI | GAANNNNTTC | 2 cut(s) | 36, 786 |
| PfeI | GAWTC | 2 cut(s) | 946, 1009 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 365 |
| PkrI | GCNGC | 3 cut(s) | 173, 307, 1174 |
| PleI | GAGTC | 1 cut(s) | 135 |
| PpsI | GAGTC | 1 cut(s) | 135 |
| Psp6I | CCWGG | 1 cut(s) | 881 |
| PspEI | GGTNACC | 1 cut(s) | 1158 |
| PspGI | CCWGG | 1 cut(s) | 881 |
| PspN4I | GGNNCC | 3 cut(s) | 544, 777, 1204 |
| PstI | CTGCAG | 2 cut(s) | 241, 592 |
| PstNI | CAGNNNCTG | 1 cut(s) | 587 |
| PsuI | RGATCY | 3 cut(s) | 209, 542, 775 |
| RsaI | GTAC | 4 cut(s) | 58, 155, 494, 1148 |
| RsaNI | GTAC | 4 cut(s) | 57, 154, 493, 1147 |
| RseI | CAYNNNNRTG | 3 cut(s) | 86, 276, 432 |
| SaqAI | TTAA | 3 cut(s) | 95, 396, 1259 |
| SatI | GCNGC | 3 cut(s) | 172, 306, 1173 |
| Sau3AI | GATC | 5 cut(s) | 209, 385, 542, 727, 775 |
| ScaI | AGTACT | 1 cut(s) | 155 |
| SchI | GAGTC | 1 cut(s) | 135 |
| ScrFI | CCNGG | 1 cut(s) | 883 |
| SfaNI | GCATC | 5 cut(s) | 587, 890, 949, 993, 1114 |
| SfcI | CTRYAG | 3 cut(s) | 237, 588, 823 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 86, 276, 432 |
| SpeI | ACTAGT | 1 cut(s) | 133 |
| SphI | GCATGC | 1 cut(s) | 1246 |
| SseBI | AGGCCT | 2 cut(s) | 886, 1051 |
| SsiI | CCGC | 3 cut(s) | 171, 845, 1172 |
| SspI | AATATT | 1 cut(s) | 1189 |
| SspMI | CTAG | 6 cut(s) | 134, 198, 599, 725, 842, 905 |
| StuI | AGGCCT | 2 cut(s) | 886, 1051 |
| StyD4I | CCNGG | 1 cut(s) | 881 |
| StyI | CCWWGG | 3 cut(s) | 286, 1071, 1109 |
| TaaI | ACNGT | 1 cut(s) | 994 |
| TaiI | ACGT | 2 cut(s) | 648, 822 |
| TaqI | TCGA | 4 cut(s) | 462, 516, 987, 1214 |
| TaqII | GACCGA | 1 cut(s) | 482 |
| TatI | WGTACW | 3 cut(s) | 56, 153, 492 |
| TauI | GCSGC | 2 cut(s) | 174, 1175 |
| TfiI | GAWTC | 2 cut(s) | 946, 1009 |
| Tru1I | TTAA | 3 cut(s) | 95, 396, 1259 |
| Tru9I | TTAA | 3 cut(s) | 95, 396, 1259 |
| TscAI | CASTG | 1 cut(s) | 753 |
| TseFI | GTSAC | 2 cut(s) | 815, 1158 |
| TseI | GCWGC | 1 cut(s) | 305 |
| Tsp45I | GTSAC | 2 cut(s) | 815, 1158 |
| TspDTI | ATGAA | 8 cut(s) | 70, 134, 294, 543, 706, 779, 1073, 1199 |
| TspRI | CASTG | 1 cut(s) | 753 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 365 |
| XapI | RAATTY | 1 cut(s) | 951 |
| XbaI | TCTAGA | 1 cut(s) | 724 |
| XceI | RCATGY | 1 cut(s) | 1246 |
| XmnI | GAANNNNTTC | 2 cut(s) | 36, 786 |
| XspI | CTAG | 6 cut(s) | 134, 198, 599, 725, 842, 905 |
| ZraI | GACGTC | 1 cut(s) | 820 |
| ZrmI | AGTACT | 1 cut(s) | 155 |