Rorug05G0046900

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000066
Physical Location & Seq
Forward (+)
4103649 .. 4108642
4994 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0046900.1

Sequence Viewer

Length: 2535 bp
ATGGGTAGCAAAAGTTGGGTGGCATTGGTTCTGTTTGTGATCATAGTTTTTGAGGGTTATTTCATTGAACCAGTAAATTCTACCACTGTGACATATGACCATAGAGCACTGGTTATTGATGGGAAGCGGCGGGTTTTGCAGTCTGGTTCTATCCATTATCCTAGAACCAACCCTGAGGTGTGGCCGGAGATTATTAATAAGGCCAAGGAAGGAGGACTTGACGTGATAGAGAGTTATGTTTTCTGGAACTATCATGAGCCTGTAAAAGGCCAGTACTACTTTGAAGGCAGGTTTGATTTGGTGAGATTTGTGAAGACAGTGCAAGAAGCTGGCCTTCTAGTTCATTTGAGGATTGGCCCTTATGCTTGTGCTGAATGGAACTACGGGGGATTTCCTATTTGGTTACACTTCATTCCCGGAATTCAATTCCGTACTACAAATGCACCTTTTAAGAATGAAATGAAGCACTTTCTTGCAAAAATTGTTGAAATGATGAAGGAGGAGCATCTCTTTGCATCACAAGGGGGGCCTATCATTCTTGCTCAGGTTGAAAATGAATATGGAAATGTCGAAGGGTCATATGGAGTTGGTGGAGAATTATATGTCAAATGGGCTGCAGAAACTGCTGTTAGTCTTAATACAAGTGTACCTTGGGTGATGTGTGTGCAAGATGATGCACCTGATCCAATCATAAACACATGCAATGGATTTTATTGTGACATGTTTGCCCCGAATTCTCCAAATAAACCGAAGATGTGGACAGAGAATTACAGCGGATGGTTCCTTGGATTCGGCAATCCAATTCCTTATAGACCTGTTGAGGATCTTGCTTTCGCTGTTGCACGGTTTTTTGAAAAAGGTGGTACATTTCAAAACTATTATATGTATTTTGGTGGGACCAACTTCGGGAGAACAGCTGGAGGCCCTTTGGTTGCAACAAGCTATGATTATGATGCTCCTATAGATGAATATGGTTTTCTTAGACAGCCAAAATGGGGCCACCTGCGTGATTTGCACAAGGCAATAAAGCACTGTGAAGAGTATCTGATCAGCTCTGATCCCACTCAAGTGCAACTTGGAGTAAATTTAGAGGCGCATATCTATTATAAACATTCCAATGAGTGTGCAGCCTTTCTTGCCAATTATGGAAGTAGATCAGATGCAAATGTCACATTCAACGGACAAGTTTATTTCCTTCCAGCTTGGTCTGTGAGCATCCTTCCAGATTGTAAGAATGTTATATTCAACACCGCCAAGGTTGTCTCACAAAAAACTCTTGGAGATCCTCTTTTTGCCAGCACAACACTGAAACCATCTTCATGGGGATGGTACAAAGAAAGAGTTGGTATTTGGAGTAACAGTTCATTCAGTAGCCCAGGCTTACTGGAGCAGATAAGTACCACGAAAGATACTAGTGATTTTTTATGGTACTCAATAAGTATCAATGTGAATGAAAACATTGCACCTGGTCAAGGGAAAGAACTGTTTTTAAATATTGAGAGCCTGGGACATGCCGCTCTAGTTTTCGTAAACAAAAAGCCTATAGGATTTGGATATGGTAACCATGAAGATGCTAGCTTCATCCTTAATGAGAAGGTTGCCCTGAACCCTGGAAACAATACCATTGATATGTTGAGTATGATGATTGGTGTACAGAACTATGGACCGTGGTTCGATGTAGCAGGAGCAGGAATTTTCTTTGTTGTTATTGCTGACTTAGAGGCTGGAAAAGTTGATCTTTCTGATGAAGAATGGACCTACCAGGTAGGAGTGGAAGGGGAATACCTTGGATTGGATAACATAAGTCTCGCAAATAGTTCACTGTGGACTACAGGGGCTACTCCACCAGTTAATCAGACTTTGATATGGTACAAGGTTTCCTTCCTTGCTCCTGAAGGGAAGGGTCCTTTGGCATTAGATCTTGCTAGCATGGGAAAGGGGCAGGCCTGGATTAATGGGCAGAGCATAGGGAGATATTGGTCCACGTATCTCTCACCATCTACTGGTTGCAACAGTAGCGGTGTTTGCGACTACAGGGGAGCCTATGATGCAAATAAATGTCTAAAGAACTGTGGTCAACCAGCTCAAACTCTGTACCATATTCCTCGTACTTGGGTGCAAGATGGAGAGAACCTTCTAGTTCTCCATGAAGAGCTTGGGGGTGACCCTTCAAAAATTTCTGTGAGGACACGAACTGGCCAAGAGATATGTGCATATGTATCAGACACTGATCCTCCGCCTGCCAGTTCTTGGAAACCAAACTCTGAATTCATGTCCCAAAATCCTGAAGTTCAACTGACCTGTGAACATGGATGGCACATTACTTCAATTAACTTTGCTAGCTATGGAACTCCTATCGGAAGCTGTGGCGCCACTTTCACTCTAGGAACTTGCAATGCTGATGTCTTAACAATCGTTGAGCAGGCTTGCCTTGGCAAAGAAGGATGTTCGATTACCATCTCCACAGAAACCTTAGGTGATCCTTGTCCAGGTGTATTAAAGAGTTTCGCTGTTCAAGCTCTTTGCAGCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK cEGF

Protein Analysis

844

Amino Acids

93.39

Weight (kDa)

5.13

Isoelectric Point (pI)

34.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_35 PF01301 37 - 341 1.6e-116 Glycosyl hydrolases family 35
Glyco_hydro_42 PF02449 57 - 190 6.2e-06 Beta-galactosidase
GHD PF17834 364 - 420 8.6e-26 Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd PF21467 621 - 711 7.9e-22 Beta-galactosidase, galactose-binding domain
SUEL_Lectin PF02140 766 - 842 6.8e-17 D-galactoside/L-rhamnose binding SUEL lectin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21210 AT1G21230 AT1G21240 AT1G21240 AT1G21245 AT1G21250 AT1G21270 AT1G22720 AT3G25490
fragaria_vesca FvH4_2g35051 FvH4_2g35051 FvH4_2g35060 FvH4_3g10281 FvH4_3g10285 FvH4_3g10286 FvH4_3g10311 FvH4_3g10311 FvH4_3g10320 FvH4_3g10332 FvH4_3g10340 FvH4_3g10350 FvH4_3g10360 FvH4_3g10390 FvH4_3g10412 FvH4_3g10413 FvH4_3g10461 FvH4_3g10462 FvH4_3g10470 FvH4_3g10510 FvH4_3g10520 FvH4_3g10530 FvH4_3g10550 FvH4_3g10560 FvH4_3g10611 FvH4_3g11730 FvH4_6g53140 FvH4_6g53142 FvH4_7g31880 FvH4_7g31910 FvH4_7g31920
malus_domestica MD05G1269900.v1.1 MD05G1270000.v1.1 MD05G1270200.v1.1 MD05G1270400.v1.1 MD05G1270500.v1.1 MD05G1270600.v1.1 MD05G1270800.v1.1 MD05G1270900.v1.1 MD07G1183800.v1.1 MD08G1107600.v1.1 MD10G1248500.v1.1 MD10G1249200.v1.1 MD10G1249500.v1.1 MD10G1250000.v1.1 MD10G1250500.v1.1 MD10G1250900.v1.1 MD10G1251200.v1.1 MD10G1251400.v1.1 MD13G1004600.v1.1 MD15G1088400.v1.1
prunus_persica Prupe.1G442400_v2.0.a1 Prupe.1G442500_v2.0.a1 Prupe.2G223400_v2.0.a1 Prupe.4G091900_v2.0.a1 Prupe.4G093200_v2.0.a1 Prupe.4G093300_v2.0.a1 Prupe.4G093400_v2.0.a1 Prupe.4G093500_v2.0.a1 Prupe.4G094100_v2.0.a1 Prupe.4G094200_v2.0.a1 Prupe.4G094300_v2.0.a1 Prupe.6G358300_v2.0.a1
pyrus_communis pycom08g08990 pycom10g20660 pycom10g20670 pycom10g20680 pycom10g20710 pycom10g20740 pycom13g00400 pycom15g08290
rosa_chinensis RchiOBHm_Chr1g0326861 RchiOBHm_Chr1g0326871 RchiOBHm_Chr1g0331391 RchiOBHm_Chr1g0336911 RchiOBHm_Chr1g0364001 RchiOBHm_Chr2g0174541 RchiOBHm_Chr2g0175041 RchiOBHm_Chr2g0175101 RchiOBHm_Chr2g0175121 RchiOBHm_Chr3g0486331 RchiOBHm_Chr5g0015581 RchiOBHm_Chr5g0016551 RchiOBHm_Chr5g0016561 RchiOBHm_Chr5g0016581 RchiOBHm_Chr5g0016601 RchiOBHm_Chr5g0016611 RchiOBHm_Chr5g0016641 RchiOBHm_Chr5g0016661 RchiOBHm_Chr5g0016691 RchiOBHm_Chr5g0016701 RchiOBHm_Chr5g0016731 RchiOBHm_Chr5g0016741 RchiOBHm_Chr5g0016811 RchiOBHm_Chr5g0016841 RchiOBHm_Chr5g0016871 RchiOBHm_Chr5g0016881 RchiOBHm_Chr5g0016931 RchiOBHm_Chr5g0017021 RchiOBHm_Chr5g0017041 RchiOBHm_Chr5g0017071 RchiOBHm_Chr5g0017091 RchiOBHm_Chr5g0017121 RchiOBHm_Chr5g0017141 RchiOBHm_Chr5g0017291 RchiOBHm_Chr5g0017321 RchiOBHm_Chr5g0017401 RchiOBHm_Chr5g0019001 RchiOBHm_Chr5g0019071 RchiOBHm_Chr5g0028941 RchiOBHm_Chr5g0052141 RchiOBHm_Chr5g0054721 RchiOBHm_Chr5g0054801 RchiOBHm_Chr5g0054931 RchiOBHm_Chr5g0054941 RchiOBHm_Chr6g0259291 RchiOBHm_Chr6g0292991 RchiOBHm_Chr6g0306911
rosa_laevigata RLG00000009465 RLG00000009468 RLG00000010755 RLG00000011976 RLG00000014541 RLG00000014542 RLG00000015169 RLG00000022308 RLG00000027529 RLG00000029324 RLG00000030244 RLG00000030245 RLG00000032210 RLG00000032211 RLG00000032257 RLG00000032259 RLG00000032260 RLG00000032261 RLG00000032262 RLG00000032268 RLG00000032269 RLG00000032272 RLG00000032282 RLG00000032283 RLG00000032308 RLG00000032413 RLG00000033129 RLG00000034945
rosa_multiflora Rmu_co8241929.1_g000001 Rmu_co8269227.1_g000001 Rmu_co8461259.1_g000001 Rmu_co8495385.1_g000001 Rmu_sc0000028.1_g000003 Rmu_sc0000083.1_g000009 Rmu_sc0000083.1_g000010 Rmu_sc0000083.1_g000031 Rmu_sc0000083.1_g000043 Rmu_sc0000083.1_g000046 Rmu_sc0000083.1_g000047 Rmu_sc0000563.1_g000024 Rmu_sc0000842.1_g000012 Rmu_sc0001235.1_g000001 Rmu_sc0001235.1_g000005 Rmu_sc0001235.1_g000009 Rmu_sc0001235.1_g000015 Rmu_sc0001235.1_g000016 Rmu_sc0001235.1_g000017 Rmu_sc0001473.1_g000036 Rmu_sc0001607.1_g000016 Rmu_sc0001788.1_g000006 Rmu_sc0002018.1_g000008 Rmu_sc0002028.1_g000001 Rmu_sc0002680.1_g000011 Rmu_sc0002798.1_g000023 Rmu_sc0002886.1_g000023 Rmu_sc0002989.1_g000012 Rmu_sc0003064.1_g000009 Rmu_sc0003382.1_g000006 Rmu_sc0003630.1_g000002 Rmu_sc0003630.1_g000047 Rmu_sc0003630.1_g000066 Rmu_sc0003630.1_g000067 Rmu_sc0003649.1_g000010 Rmu_sc0003688.1_g000005 Rmu_sc0003920.1_g000007 Rmu_sc0004765.1_g000007 Rmu_sc0004765.1_g000008 Rmu_sc0004920.1_g000011 Rmu_sc0005223.1_g000008 Rmu_sc0005223.1_g000010 Rmu_sc0005223.1_g000015 Rmu_sc0005223.1_g000016 Rmu_sc0005319.1_g000014 Rmu_sc0005319.1_g000015 Rmu_sc0005319.1_g000018 Rmu_sc0005319.1_g000019 Rmu_sc0005319.1_g000025 Rmu_sc0005418.1_g000007 Rmu_sc0005418.1_g000012 Rmu_sc0006106.1_g000011 Rmu_sc0006914.1_g000012 Rmu_sc0007023.1_g000001 Rmu_sc0007662.1_g000001 Rmu_sc0007920.1_g000002 Rmu_sc0007920.1_g000013 Rmu_sc0008053.1_g000016 Rmu_sc0008053.1_g000017 Rmu_sc0008053.1_g000018 Rmu_sc0008053.1_g000023 Rmu_sc0008053.1_g000050 Rmu_sc0009042.1_g000007 Rmu_sc0009042.1_g000008 Rmu_sc0010642.1_g000008 Rmu_sc0011109.1_g000002 Rmu_sc0011314.1_g000006 Rmu_sc0011974.1_g000006 Rmu_sc0012179.1_g000009 Rmu_sc0015169.1_g000001 Rmu_sc0016368.1_g000002 Rmu_sc0022659.1_g000001 Rmu_sc0027371.1_g000001 Rmu_sc0028213.1_g000001 Rmu_sc0034526.1_g000001 Rmu_sc0039366.1_g000002 Rmu_ssc0000050.1_g000084 Rmu_ssc0000050.1_g000088 Rmu_ssc0000167.1_g000017
rosa_roxburghii Rroxscaffold_1G00011340 Rroxscaffold_1G00025710 Rroxscaffold_1G00025870 Rroxscaffold_1G00026070 Rroxscaffold_1G00026170 Rroxscaffold_1G00026200 Rroxscaffold_1G00026270 Rroxscaffold_1G00050950 Rroxscaffold_1G00058900 Rroxscaffold_1G00060040 Rroxscaffold_1G00060060 Rroxscaffold_1G00060070 Rroxscaffold_1G00060080 Rroxscaffold_1G00060110 Rroxscaffold_1G00060120 Rroxscaffold_1G00060140 Rroxscaffold_1G00060170 Rroxscaffold_1G00060210 Rroxscaffold_1G00060220 Rroxscaffold_1G00060240 Rroxscaffold_1G00060250 Rroxscaffold_1G00060260 Rroxscaffold_1G00060300 Rroxscaffold_1G00060320 Rroxscaffold_1G00060330 Rroxscaffold_1G00060900 Rroxscaffold_1G00060920 Rroxscaffold_1G00073980 Rroxscaffold_2G00077560 Rroxscaffold_2G00077580 Rroxscaffold_2G00146990 Rroxscaffold_3G00236200 Rroxscaffold_3G00236210 Rroxscaffold_3G00236220 Rroxscaffold_4G00292760 Rroxscaffold_4G00315200 Rroxscaffold_4G00315240 Rroxscaffold_4G00326240 Rroxscaffold_4G00328980 Rroxscaffold_5G00341890 Rroxscaffold_7G00161350 Rroxscaffold_7G00174490 Rroxscaffold_7G00206550
rosa_rugosa Rorug01G0093000 Rorug01G0093100 Rorug01G0128800 Rorug01G0312000 Rorug01G0312000 Rorug02G0581100 Rorug03G0224600 Rorug05G0028500 Rorug05G0032700 Rorug05G0032800 Rorug05G0032900 Rorug05G0032900 Rorug05G0033000 Rorug05G0033100 Rorug05G0033300 Rorug05G0033400 Rorug05G0033900 Rorug05G0034900 Rorug05G0035000 Rorug05G0046900 Rorug05G0047500 Rorug05G0047500 Rorug05G0111600 Rorug05G0287900 Rorug05G0288700 Rorug05G0586900 Rorug06G0231700 Rorug06G0354300 Rorug06G0354400
rosa_samantha Rh1DG122200 Rh2DG686800 Rh5BG136600 Rh6AG344500 Rh6BG351900 Rh6CG359000 Rh6CG359100 Rh6CG387600 Rh6CG480500 Rh6DG344600 Rh6DG467400
rosa_wichuraiana Rw0G010250 Rw0G014820 Rw0G021400 Rw0G023100 Rw1G009450 Rw1G012100 Rw1G012180 Rw1G028360 Rw2G054260 Rw3G024370 Rw3G024420 Rw3G024470 Rw5G010850 Rw5G011010 Rw5G011020 Rw5G011050 Rw5G011080 Rw5G011120 Rw5G012300 Rw5G018470 Rw5G033830 Rw6G008930 Rw6G030070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1107
AarI CACCTGC 1 cut(s) 1011
Acc36I ACCTGC 2 cut(s) 279, 1011
AccB1I GGYRCC 1 cut(s) 2369
AccB7I CCANNNNNTGG 2 cut(s) 2003, 2250
AccBSI CCGCTC 1 cut(s) 1517
AciI CCGC 7 cut(s) 127, 130, 774, 1251, 1515, 2019, 2237
AclWI GGATC 6 cut(s) 677, 831, 1052, 1277, 2225, 2474
AcoI YGGCCR 2 cut(s) 182, 2197
AcsI RAATTY 7 cut(s) 76, 420, 733, 1084, 1692, 2175, 2267
AcuI CTGAAG 2 cut(s) 1914, 2307
AcyI GRCGYC 1 cut(s) 2370
AfiI CCNNNNNNNGG 8 cut(s) 266, 906, 995, 1472, 1792, 2003, 2250, 2489
AflIII ACRYGT 1 cut(s) 720
AhlI ACTAGT 1 cut(s) 1412
AjiI CACGTC 1 cut(s) 223
AjnI CCWGG 7 cut(s) 1375, 1465, 1503, 1609, 1761, 1946, 2488
AjuI GAANNNNNNNTTGG 2 cut(s) 1892, 1924
AleI CACNNNNGTG 2 cut(s) 1005, 1067
AloI GAACNNNNNNTCC 2 cut(s) 1345, 1377
Alw21I GWGCWC 1 cut(s) 109
Alw26I GTCTC 2 cut(s) 1267, 1811
AlwI GGATC 6 cut(s) 677, 831, 1052, 1277, 2225, 2474
AlwNI CAGNNNCTG 2 cut(s) 623, 2228
ApeKI GCWGC 3 cut(s) 614, 1127, 2526
ApoI RAATTY 7 cut(s) 76, 420, 733, 1084, 1692, 2175, 2267
AseI ATTAAT 2 cut(s) 195, 1953
AspLEI GCGC 2 cut(s) 1096, 2372
AspS9I GGNCC 9 cut(s) 356, 527, 897, 923, 997, 1664, 1755, 1904, 1980
AsuC2I CCSGG 1 cut(s) 417
AsuHPI GGTGA 5 cut(s) 313, 667, 1986, 2174, 2489
AsuNHI GCTAGC 3 cut(s) 1574, 1925, 2339
AvaII GGWCC 5 cut(s) 897, 1664, 1755, 1904, 1980
AxyI CCTNAGG 2 cut(s) 174, 2473
BaeI ACNNNNGTAYC 2 cut(s) 630, 663
BalI TGGCCA 1 cut(s) 2199
BanI GGYRCC 1 cut(s) 2369
BbsI GAAGAC 1 cut(s) 320
Bbv12I GWGCWC 1 cut(s) 109
BbvI GCAGC 2 cut(s) 601, 1139
BccI CCATC 8 cut(s) 113, 771, 1320, 1321, 2005, 2117, 2307, 2465
BciT130I CCWGG 7 cut(s) 1377, 1467, 1505, 1611, 1763, 1948, 2490
BclI TGATCA 2 cut(s) 39, 1047
BcnI CCSGG 1 cut(s) 417
BcoDI GTCTC 2 cut(s) 1267, 1811
BcuI ACTAGT 1 cut(s) 1412
BfaI CTAG 9 cut(s) 162, 338, 1413, 1520, 1575, 1926, 2138, 2340, 2384
BfmI CTRYAG 5 cut(s) 615, 960, 1542, 1830, 2032
BfoI RGCGCY 1 cut(s) 2373
BfuAI ACCTGC 2 cut(s) 279, 1011
BglII AGATCT 1 cut(s) 1918
BisI GCNGC 5 cut(s) 128, 615, 1128, 1515, 2527
BlsI GCNGC 5 cut(s) 129, 616, 1129, 1516, 2528
BmcAI AGTACT 1 cut(s) 275
Bme1390I CCNGG 8 cut(s) 417, 1377, 1467, 1505, 1611, 1763, 1948, 2490
Bme18I GGWCC 5 cut(s) 897, 1664, 1755, 1904, 1980
BmgBI CACGTC 1 cut(s) 223
BmgT120I GGNCC 9 cut(s) 356, 527, 897, 923, 997, 1664, 1755, 1904, 1980
BmiI GGNNCC 7 cut(s) 528, 782, 898, 998, 1905, 2041, 2371
BmrFI CCNGG 8 cut(s) 417, 1377, 1467, 1505, 1611, 1763, 1948, 2490
BmsI GCATC 8 cut(s) 514, 524, 664, 943, 1150, 1224, 1561, 2038
BmtI GCTAGC 3 cut(s) 1578, 1929, 2343
BpiI GAAGAC 1 cut(s) 320
BpmI CTGGAG 2 cut(s) 939, 1406
Bpu10I CCTNAGC 1 cut(s) 543
BpuEI CTTGAG 1 cut(s) 1050
BpuMI CCSGG 1 cut(s) 417
BsaAI YACGTR 1 cut(s) 1986
BsaBI GATNNNNATC 1 cut(s) 2456
BsaHI GRCGYC 1 cut(s) 2370
BsaXI ACNNNNNCTCC 4 cut(s) 1345, 1375, 2218, 2248
Bsc4I CCNNNNNNNGG 8 cut(s) 266, 906, 995, 1472, 1792, 2003, 2250, 2489
Bse1I ACTGG 8 cut(s) 71, 114, 271, 1389, 1847, 2008, 2200, 2244
Bse21I CCTNAGG 2 cut(s) 174, 2473
Bse3DI GCAATG 3 cut(s) 709, 1458, 2401
Bse8I GATNNNNATC 1 cut(s) 2456
BseBI CCWGG 7 cut(s) 1377, 1467, 1505, 1611, 1763, 1948, 2490
BseGI GGATG 6 cut(s) 782, 1215, 1331, 1581, 2318, 2450
BseJI GATNNNNATC 1 cut(s) 2456
BseLI CCNNNNNNNGG 8 cut(s) 266, 906, 995, 1472, 1792, 2003, 2250, 2489
BseMI GCAATG 3 cut(s) 709, 1458, 2401
BseMII CTCAG 2 cut(s) 165, 557
BseNI ACTGG 8 cut(s) 71, 114, 271, 1389, 1847, 2008, 2200, 2244
BseRI GAGGAG 1 cut(s) 515
BseXI GCAGC 2 cut(s) 601, 1139
BsgI GTGCAG 1 cut(s) 1146
BshNI GGYRCC 1 cut(s) 2369
BsiHKAI GWGCWC 1 cut(s) 109
BsiSI CCGG 2 cut(s) 185, 417
BslFI GGGAC 3 cut(s) 910, 1521, 2260
BslI CCNNNNNNNGG 8 cut(s) 266, 906, 995, 1472, 1792, 2003, 2250, 2489
BsmAI GTCTC 2 cut(s) 1267, 1811
BsmFI GGGAC 3 cut(s) 910, 1521, 2260
Bsp1286I GDGCHC 1 cut(s) 109
Bsp1407I TGTACA 1 cut(s) 1651
BspACI CCGC 7 cut(s) 127, 130, 774, 1251, 1515, 2019, 2237
BspCNI CTCAG 2 cut(s) 166, 556
BspHI TCATGA 1 cut(s) 253
BspLI GGNNCC 7 cut(s) 528, 782, 898, 998, 1905, 2041, 2371
BspMAI CTGCAG 1 cut(s) 619
BspMI ACCTGC 2 cut(s) 279, 1011
BspOI GCTAGC 3 cut(s) 1578, 1929, 2343
BspPI GGATC 6 cut(s) 677, 831, 1052, 1277, 2225, 2474
BspQI GCTCTTC 1 cut(s) 2145
BspT107I GGYRCC 1 cut(s) 2369
BsrBI CCGCTC 1 cut(s) 1517
BsrDI GCAATG 3 cut(s) 709, 1458, 2401
BsrGI TGTACA 1 cut(s) 1651
BsrI ACTGG 8 cut(s) 71, 114, 271, 1389, 1847, 2008, 2200, 2244
BssNI GRCGYC 1 cut(s) 2370
BssT1I CCWWGG 6 cut(s) 204, 650, 784, 1254, 1786, 2431
Bst2UI CCWGG 7 cut(s) 1377, 1467, 1505, 1611, 1763, 1948, 2490
Bst6I CTCTTC 2 cut(s) 1032, 2145
BstACI GRCGYC 1 cut(s) 2370
BstAPI GCANNNNNTGC 1 cut(s) 623
BstAUI TGTACA 1 cut(s) 1651
BstBAI YACGTR 1 cut(s) 1986
BstC8I GCNNGC 9 cut(s) 331, 1297, 1576, 1927, 1944, 2241, 2341, 2424, 2428
BstDEI CTNAG 5 cut(s) 174, 543, 980, 1717, 2473
BstDSI CCRYGG 1 cut(s) 1667
BstEII GGTNACC 2 cut(s) 1559, 2162
BstENI CCTNNNNNAGG 3 cut(s) 264, 1470, 2487
BstF5I GGATG 6 cut(s) 782, 1215, 1331, 1581, 2318, 2450
BstH2I RGCGCY 1 cut(s) 2373
BstHHI GCGC 2 cut(s) 1096, 2372
BstMAI GTCTC 2 cut(s) 1267, 1811
BstMWI GCNNNNNNNGC 8 cut(s) 136, 623, 1012, 1136, 2016, 2025, 2048, 2516
BstNI CCWGG 7 cut(s) 1377, 1467, 1505, 1611, 1763, 1948, 2490
BstNSI RCATGY 3 cut(s) 702, 724, 1514
BstPI GGTNACC 2 cut(s) 1559, 2162
BstSCI CCNGG 8 cut(s) 415, 1375, 1465, 1503, 1609, 1761, 1946, 2488
BstSFI CTRYAG 5 cut(s) 615, 960, 1542, 1830, 2032
BstV1I GCAGC 2 cut(s) 601, 1139
BstV2I GAAGAC 1 cut(s) 320
BstX2I RGATCY 3 cut(s) 823, 1282, 1918
BstXI CCANNNNNNTGG 1 cut(s) 1320
BstYI RGATCY 3 cut(s) 823, 1282, 1918
Bsu36I CCTNAGG 2 cut(s) 174, 2473
BtgI CCRYGG 1 cut(s) 1667
BtrI CACGTC 1 cut(s) 223
BtsCI GGATG 6 cut(s) 782, 1215, 1331, 1581, 2318, 2450
BtsIMutI CAGTG 7 cut(s) 84, 107, 324, 1030, 1304, 1820, 2226
BveI ACCTGC 2 cut(s) 279, 1011
Cac8I GCNNGC 9 cut(s) 331, 1297, 1576, 1927, 1944, 2241, 2341, 2424, 2428
CaiI CAGNNNCTG 2 cut(s) 623, 2228
CciI TCATGA 1 cut(s) 253
CfoI GCGC 2 cut(s) 1096, 2372
Cfr13I GGNCC 9 cut(s) 356, 527, 897, 923, 997, 1664, 1755, 1904, 1980
CsiI ACCWGGT 2 cut(s) 1465, 1761
DdeI CTNAG 5 cut(s) 174, 543, 980, 1717, 2473
DinI GGCGCC 1 cut(s) 2371
DraI TTTAAA 1 cut(s) 1491
EaeI YGGCCR 2 cut(s) 182, 2197
Eam1104I CTCTTC 2 cut(s) 1032, 2145
EarI CTCTTC 2 cut(s) 1032, 2145
EciI GGCGGA 1 cut(s) 2226
Eco130I CCWWGG 6 cut(s) 204, 650, 784, 1254, 1786, 2431
Eco147I AGGCCT 1 cut(s) 1946
Eco47I GGWCC 5 cut(s) 897, 1664, 1755, 1904, 1980
Eco57I CTGAAG 2 cut(s) 1914, 2307
Eco81I CCTNAGG 2 cut(s) 174, 2473
Eco91I GGTNACC 2 cut(s) 1559, 2162
EcoNI CCTNNNNNAGG 3 cut(s) 264, 1470, 2487
EcoO109I RGGNCCY 3 cut(s) 527, 923, 1904
EcoO65I GGTNACC 2 cut(s) 1559, 2162
EcoRI GAATTC 3 cut(s) 420, 733, 2267
EcoRII CCWGG 7 cut(s) 1375, 1465, 1503, 1609, 1761, 1946, 2488
EcoT14I CCWWGG 6 cut(s) 204, 650, 784, 1254, 1786, 2431
EgeI GGCGCC 1 cut(s) 2371
EheI GGCGCC 1 cut(s) 2371
ErhI CCWWGG 6 cut(s) 204, 650, 784, 1254, 1786, 2431
FaqI GGGAC 3 cut(s) 910, 1521, 2260
FauI CCCGC 1 cut(s) 123
FauNDI CATATG 3 cut(s) 94, 580, 2215
FbaI TGATCA 2 cut(s) 39, 1047
Fnu4HI GCNGC 5 cut(s) 128, 615, 1128, 1515, 2527
FokI GGATG 6 cut(s) 789, 1202, 1338, 1568, 2325, 2457
Fsp4HI GCNGC 5 cut(s) 128, 615, 1128, 1515, 2527
FspBI CTAG 9 cut(s) 162, 338, 1413, 1520, 1575, 1926, 2138, 2340, 2384
GlaI GCGC 2 cut(s) 1095, 2371
GluI GCNGC 5 cut(s) 128, 615, 1128, 1515, 2527
GsuI CTGGAG 2 cut(s) 939, 1406
HaeII RGCGCY 1 cut(s) 2373
HapII CCGG 2 cut(s) 185, 417
HhaI GCGC 2 cut(s) 1096, 2372
Hin1I GRCGYC 1 cut(s) 2370
Hin6I GCGC 2 cut(s) 1094, 2370
HinP1I GCGC 2 cut(s) 1094, 2370
HincII GTYRAC 1 cut(s) 2078
HindII GTYRAC 1 cut(s) 2078
HinfI GANTC 1 cut(s) 789
HpaII CCGG 2 cut(s) 185, 417
HphI GGTGA 5 cut(s) 313, 667, 1986, 2174, 2489
Hpy166II GTNNAC 9 cut(s) 647, 759, 1531, 1652, 1820, 1827, 1983, 2078, 2306
Hpy188I TCNGA 8 cut(s) 1047, 1057, 1159, 1744, 1857, 2224, 2266, 2360
Hpy188III TCNNGA 6 cut(s) 244, 254, 907, 1223, 1892, 2285
Hpy8I GTNNAC 9 cut(s) 647, 759, 1531, 1652, 1820, 1827, 1983, 2078, 2306
HpyCH4IV ACGT 2 cut(s) 222, 1985
HpyF10VI GCNNNNNNNGC 8 cut(s) 136, 623, 1012, 1136, 2016, 2025, 2048, 2516
HpyF3I CTNAG 5 cut(s) 174, 543, 980, 1717, 2473
HpySE526I ACGT 2 cut(s) 222, 1985
Hsp92I GRCGYC 1 cut(s) 2370
HspAI GCGC 2 cut(s) 1094, 2370
KasI GGCGCC 1 cut(s) 2369
Ksp22I TGATCA 2 cut(s) 39, 1047
LguI GCTCTTC 1 cut(s) 2145
LmnI GCTCC 6 cut(s) 502, 961, 1387, 1685, 1894, 2039
Lsp1109I GCAGC 2 cut(s) 601, 1139
LweI GCATC 8 cut(s) 514, 524, 664, 943, 1150, 1224, 1561, 2038
MabI ACCWGGT 2 cut(s) 1465, 1761
MaeI CTAG 9 cut(s) 162, 338, 1413, 1520, 1575, 1926, 2138, 2340, 2384
MaeII ACGT 2 cut(s) 222, 1985
MaeIII GTNAC 7 cut(s) 88, 402, 716, 1168, 1355, 1559, 2162
MbiI CCGCTC 1 cut(s) 1517
MboII GAAGA 7 cut(s) 325, 763, 1049, 1308, 1580, 1760, 2162
MfeI CAATTG 1 cut(s) 2530
MflI RGATCY 3 cut(s) 823, 1282, 1918
MhlI GDGCHC 1 cut(s) 109
MlsI TGGCCA 1 cut(s) 2199
MluNI TGGCCA 1 cut(s) 2199
Mly113I GGCGCC 1 cut(s) 2370
Mox20I TGGCCA 1 cut(s) 2199
MscI TGGCCA 1 cut(s) 2199
MslI CAYNNNNRTG 7 cut(s) 1005, 1067, 1116, 1318, 1324, 1569, 1628
Msp20I TGGCCA 1 cut(s) 2199
MspA1I CMGCKG 2 cut(s) 774, 917
MspI CCGG 2 cut(s) 185, 417
MspR9I CCNGG 8 cut(s) 417, 1377, 1467, 1505, 1611, 1763, 1948, 2490
MunI CAATTG 1 cut(s) 2530
MvaI CCWGG 7 cut(s) 1377, 1467, 1505, 1611, 1763, 1948, 2490
MwoI GCNNNNNNNGC 8 cut(s) 136, 623, 1012, 1136, 2016, 2025, 2048, 2516
NarI GGCGCC 1 cut(s) 2370
NciI CCSGG 1 cut(s) 417
NdeI CATATG 3 cut(s) 94, 580, 2215
NheI GCTAGC 3 cut(s) 1574, 1925, 2339
NlaIV GGNNCC 7 cut(s) 528, 782, 898, 998, 1905, 2041, 2371
NmuCI GTSAC 4 cut(s) 88, 716, 1168, 2162
NspI RCATGY 3 cut(s) 702, 724, 1514
OliI CACNNNNGTG 2 cut(s) 1005, 1067
PagI TCATGA 1 cut(s) 253
PaqCI CACCTGC 1 cut(s) 1011
PceI AGGCCT 1 cut(s) 1946
PciI ACATGT 1 cut(s) 720
PciSI GCTCTTC 1 cut(s) 2145
PfeI GAWTC 1 cut(s) 789
PflMI CCANNNNNTGG 2 cut(s) 2003, 2250
PfoI TCCNGGA 1 cut(s) 415
PkrI GCNGC 5 cut(s) 129, 616, 1129, 1516, 2528
PluTI GGCGCC 1 cut(s) 2373
Ppu21I YACGTR 1 cut(s) 1986
PpuMI RGGWCCY 1 cut(s) 1904
PscI ACATGT 1 cut(s) 720
PshBI ATTAAT 2 cut(s) 195, 1953
PsiI TTATAA 1 cut(s) 1107
Psp5II RGGWCCY 1 cut(s) 1904
Psp6I CCWGG 7 cut(s) 1375, 1465, 1503, 1609, 1761, 1946, 2488
PspEI GGTNACC 2 cut(s) 1559, 2162
PspGI CCWGG 7 cut(s) 1375, 1465, 1503, 1609, 1761, 1946, 2488
PspN4I GGNNCC 7 cut(s) 528, 782, 898, 998, 1905, 2041, 2371
PspPI GGNCC 9 cut(s) 356, 527, 897, 923, 997, 1664, 1755, 1904, 1980
PspPPI RGGWCCY 1 cut(s) 1904
PstI CTGCAG 1 cut(s) 619
PstNI CAGNNNCTG 2 cut(s) 623, 2228
PsuI RGATCY 3 cut(s) 823, 1282, 1918
PvuII CAGCTG 1 cut(s) 917
RseI CAYNNNNRTG 7 cut(s) 1005, 1067, 1116, 1318, 1324, 1569, 1628
SapI GCTCTTC 1 cut(s) 2145
SatI GCNGC 5 cut(s) 128, 615, 1128, 1515, 2527
Sau96I GGNCC 9 cut(s) 356, 527, 897, 923, 997, 1664, 1755, 1904, 1980
ScaI AGTACT 1 cut(s) 275
ScrFI CCNGG 8 cut(s) 417, 1377, 1467, 1505, 1611, 1763, 1948, 2490
SduI GDGCHC 1 cut(s) 109
SexAI ACCWGGT 2 cut(s) 1465, 1761
SfaNI GCATC 8 cut(s) 514, 524, 664, 943, 1150, 1224, 1561, 2038
SfcI CTRYAG 5 cut(s) 615, 960, 1542, 1830, 2032
SfoI GGCGCC 1 cut(s) 2371
SinI GGWCC 5 cut(s) 897, 1664, 1755, 1904, 1980
SmiMI CAYNNNNRTG 7 cut(s) 1005, 1067, 1116, 1318, 1324, 1569, 1628
SmlI CTYRAG 1 cut(s) 1065
SmoI CTYRAG 1 cut(s) 1065
SpeI ACTAGT 1 cut(s) 1412
SseBI AGGCCT 1 cut(s) 1946
SsiI CCGC 7 cut(s) 127, 130, 774, 1251, 1515, 2019, 2237
SspDI GGCGCC 1 cut(s) 2369
SspI AATATT 1 cut(s) 1495
SspMI CTAG 9 cut(s) 162, 338, 1413, 1520, 1575, 1926, 2138, 2340, 2384
StuI AGGCCT 1 cut(s) 1946
StyD4I CCNGG 8 cut(s) 415, 1375, 1465, 1503, 1609, 1761, 1946, 2488
StyI CCWWGG 6 cut(s) 204, 650, 784, 1254, 1786, 2431
TaiI ACGT 2 cut(s) 225, 1988
TaqI TCGA 3 cut(s) 570, 1674, 2450
TatI WGTACW 2 cut(s) 273, 1651
TauI GCSGC 2 cut(s) 130, 1517
TfiI GAWTC 1 cut(s) 789
TscAI CASTG 7 cut(s) 91, 114, 324, 1037, 1311, 1827, 2233
TseFI GTSAC 4 cut(s) 88, 716, 1168, 2162
TseI GCWGC 3 cut(s) 614, 1127, 2526
Tsp45I GTSAC 4 cut(s) 88, 716, 1168, 2162
TspGWI ACGGA 2 cut(s) 419, 1194
TspRI CASTG 7 cut(s) 91, 114, 324, 1037, 1311, 1827, 2233
Van91I CCANNNNNTGG 2 cut(s) 2003, 2250
VpaK11BI GGWCC 5 cut(s) 897, 1664, 1755, 1904, 1980
VspI ATTAAT 2 cut(s) 195, 1953
XagI CCTNNNNNAGG 3 cut(s) 264, 1470, 2487
XapI RAATTY 7 cut(s) 76, 420, 733, 1084, 1692, 2175, 2267
XceI RCATGY 3 cut(s) 702, 724, 1514
XcmI CCANNNNNNNNNTGG 1 cut(s) 2153
XspI CTAG 9 cut(s) 162, 338, 1413, 1520, 1575, 1926, 2138, 2340, 2384
ZrmI AGTACT 1 cut(s) 275
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.