Rw5G018470

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000066
Physical Location & Seq
Forward (+)
23869435 .. 23872003
2569 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G018470.1

Sequence Viewer

Length: 2322 bp
ATGGCCTTTTTACACTGGAGGATGCAGATGACGAAACTCTCTTTGGCTATATTATTGGCAGCTGTAACAACAAGAGCAGCAGCCGATCAAGCCCTACCTGGTTGTGCAGAAAAGTGCGGTGATATAACAATCCCATATCCATTTGGGATAGGCGATGGCTGCTACCTGCGTCCAGAATTCAACATCAATTGTAATGGATCCACCACACCCACGTCAACAAACTTTGCAAACAGTACCTATACTTTTCCCATAGCCATAGCTAACTTTTCCATTTCTGAGGGTGAGTTGCAAGTCATGCAGGCCGTAGCCCTAGATTGTTATGACAACAAGGGTGTAAAGATAGCCAACAGCACAAGTACGCTGGAGCTGCCGCCCTCTTATACCATCTCTGCAAAAAACAAGTTCTTTCTCGTTGGCTGTAATAATGCAGCAATTTATGAAGGTCTACTTCAGTTCCCAGACCCAGATGAAGGAAGTGACTTCCAAGCAGCCGGGTTCACTGTGACCTTGTGTGTAAATGAGCTTGGCAAAGAGTTGCACGAGTCTTGTGACGGCTTTGGGTGTGCCCAAACTTCCATCCTTAGTGGACTCCAAAATATCACGGTGTCCTTGGATGCACTTGATTCTGATAACCGAACAGATTCGTGGAGCTTAAAGTACCTGTGCAGCTACGCATTCATTGTGGACGAAGGCAATTTCACATTCTCCCCGGAGAGAAGTTTCCAACAATTGAATACCACAAGGCAGCTACTTCCAGTACTTATTAACTGGGCGATTGGGGACGAGCCATGTGAAGTAGCAAAGAAGAACCACAGTACTTATGCATGCAAAGAAAATACCACCTGTGTCGACCGAAGTACCATCACAAATGGTACGGTTGGTTACCTTTGCCACTGCTTGCCAGGTTACCATGGAAATCCGTACCTTGGTTGCAAAGATATCGATGAGTGCCAGGCTTCAACTAACCCCTGCCAGAATGGGAAGTGCATAAACTCACCTCCAGGAAATTATTCTTGTCAATGTAACGGAGGGTACAAAAACAAAGATCCAATAACTTGCGTTGAATATCCCAAAGCAAAGAACACTTCCCTGAAAATTTCATTCGGTCTCATTGTAGGATTCTTGGTTTTAGTCGTTTCAATGTCATCCATATATTGGATAATGAACAAAAGAAGGCTCATCAAGCTCAAAGACAAGTACTTTAAAGAAAATGGCGGCTCAACATTACAGCAACGGCTAGCTACTCATGGAGGAGGAGTGGAGACAACAAAAATCTTTACGGCTGAAGAGCTTAAGAAGGCAACGAACAATTATAATGCAGGTGAAATTCTTGGTGAAGGAGGCTACGGAACAGTTTACAAAGGAATATTACCTGATAAAAGAGTTGTAGCCATCAAAAAGTCAAAACTGGGAACCCCAACTCAAAGTGATCAATTTGTCAATGAGGTGATTATTCTTTCTCAAATCAACCACAGAAACGTGGTAAAATTATTAGGTTGTTGTTTTGAGACTGAAGTGCCTTTACTAGTATACGAGTACATCACCGGTGGAACTCTTTCTGATCATGTATTCAAGAAAAGACAAAGATCTGTGCTTTCATGGGAATTACGACTGAAGATTGCATCAGAAACGGCAGGGGCACTTGCATACTTGCACTCCTCTACTTCTACTCCAATCATACATCGAGACGTGAAATCAACGAATATATTATTGGAGGACAATTACACGGCCAAAGTGTCAGACTTTGGAGCTTCACGTTTCATTCCTATAGATCAAACTCAAGTAGCAACACTAGTGCAAGGGACACTTGGATACTTGGACCCTGAATACTGCCATTCAAACCAACTGACTGAAAAGAGTGATGTATATAGCTTTGGTGTTGTGCTAGCGGAGCTACTAACGAGGAAAGTGGCAGTTTCTTTTGCTAGGCCCGAGGAAGAGAGAAACCTAGCAAGTTTGTTTATTTCTTCAATGGAAAAAGAATGCTTGAATCAAATTCTTGATGCTGAAATTCTGAATGAGAGGAATATGGAGACAATACAGCAAGTGGCGAATCTGGCGAAAAGATGTTTGAGGATAAAAGGAGAGGAAAGGCCTTTGATGAAAGAAGTAGCTACGGAGTTGGAGGGGATGAGAATTGAGGCAAAACATCCATGGAAATGTGGTAGTTCCTCCTCTGAGGGGACTGAGTTCCTGTTAGGGTCATCCAATTCGAAGACTAATGTTGCGGATACTAAAGGTGAAAGTGTTTCTAGTGGTACGACCAGCGGGTATGCCAGTATGCAAATTCAGATGTTAATGTCACATGAACATGGGCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK hEGF

Protein Analysis

773

Amino Acids

84.9

Weight (kDa)

5.96

Isoelectric Point (pI)

31.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 35 - 93 6.6e-10 Wall-associated receptor kinase galacturonan-binding
EGF_CA PF07645 313 - 353 3.4e-06 Calcium-binding EGF domain
Pkinase PF00069 438 - 705 4.5e-46 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 441 - 708 2.1e-47 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21210 AT1G21230 AT1G21240 AT1G21240 AT1G21245 AT1G21250 AT1G21270 AT1G22720 AT3G25490
fragaria_vesca FvH4_2g35051 FvH4_2g35051 FvH4_2g35060 FvH4_3g10281 FvH4_3g10285 FvH4_3g10286 FvH4_3g10311 FvH4_3g10311 FvH4_3g10320 FvH4_3g10332 FvH4_3g10340 FvH4_3g10350 FvH4_3g10360 FvH4_3g10390 FvH4_3g10412 FvH4_3g10413 FvH4_3g10461 FvH4_3g10462 FvH4_3g10470 FvH4_3g10510 FvH4_3g10520 FvH4_3g10530 FvH4_3g10550 FvH4_3g10560 FvH4_3g10611 FvH4_3g11730 FvH4_6g53140 FvH4_6g53142 FvH4_7g31880 FvH4_7g31910 FvH4_7g31920
malus_domestica MD05G1269900.v1.1 MD05G1270000.v1.1 MD05G1270200.v1.1 MD05G1270400.v1.1 MD05G1270500.v1.1 MD05G1270600.v1.1 MD05G1270800.v1.1 MD05G1270900.v1.1 MD07G1183800.v1.1 MD08G1107600.v1.1 MD10G1248500.v1.1 MD10G1249200.v1.1 MD10G1249500.v1.1 MD10G1250000.v1.1 MD10G1250500.v1.1 MD10G1250900.v1.1 MD10G1251200.v1.1 MD10G1251400.v1.1 MD13G1004600.v1.1 MD15G1088400.v1.1
prunus_persica Prupe.1G442400_v2.0.a1 Prupe.1G442500_v2.0.a1 Prupe.2G223400_v2.0.a1 Prupe.4G091900_v2.0.a1 Prupe.4G093200_v2.0.a1 Prupe.4G093300_v2.0.a1 Prupe.4G093400_v2.0.a1 Prupe.4G093500_v2.0.a1 Prupe.4G094100_v2.0.a1 Prupe.4G094200_v2.0.a1 Prupe.4G094300_v2.0.a1 Prupe.6G358300_v2.0.a1
pyrus_communis pycom08g08990 pycom10g20660 pycom10g20670 pycom10g20680 pycom10g20710 pycom10g20740 pycom13g00400 pycom15g08290
rosa_chinensis RchiOBHm_Chr1g0326861 RchiOBHm_Chr1g0326871 RchiOBHm_Chr1g0331391 RchiOBHm_Chr1g0336911 RchiOBHm_Chr1g0364001 RchiOBHm_Chr2g0174541 RchiOBHm_Chr2g0175041 RchiOBHm_Chr2g0175101 RchiOBHm_Chr2g0175121 RchiOBHm_Chr3g0486331 RchiOBHm_Chr5g0015581 RchiOBHm_Chr5g0016551 RchiOBHm_Chr5g0016561 RchiOBHm_Chr5g0016581 RchiOBHm_Chr5g0016601 RchiOBHm_Chr5g0016611 RchiOBHm_Chr5g0016641 RchiOBHm_Chr5g0016661 RchiOBHm_Chr5g0016691 RchiOBHm_Chr5g0016701 RchiOBHm_Chr5g0016731 RchiOBHm_Chr5g0016741 RchiOBHm_Chr5g0016811 RchiOBHm_Chr5g0016841 RchiOBHm_Chr5g0016871 RchiOBHm_Chr5g0016881 RchiOBHm_Chr5g0016931 RchiOBHm_Chr5g0017021 RchiOBHm_Chr5g0017041 RchiOBHm_Chr5g0017071 RchiOBHm_Chr5g0017091 RchiOBHm_Chr5g0017121 RchiOBHm_Chr5g0017141 RchiOBHm_Chr5g0017291 RchiOBHm_Chr5g0017321 RchiOBHm_Chr5g0017401 RchiOBHm_Chr5g0019001 RchiOBHm_Chr5g0019071 RchiOBHm_Chr5g0028941 RchiOBHm_Chr5g0052141 RchiOBHm_Chr5g0054721 RchiOBHm_Chr5g0054801 RchiOBHm_Chr5g0054931 RchiOBHm_Chr5g0054941 RchiOBHm_Chr6g0259291 RchiOBHm_Chr6g0292991 RchiOBHm_Chr6g0306911
rosa_laevigata RLG00000009465 RLG00000009468 RLG00000010755 RLG00000011976 RLG00000014541 RLG00000014542 RLG00000015169 RLG00000022308 RLG00000027529 RLG00000029324 RLG00000030244 RLG00000030245 RLG00000032210 RLG00000032211 RLG00000032257 RLG00000032259 RLG00000032260 RLG00000032261 RLG00000032262 RLG00000032268 RLG00000032269 RLG00000032272 RLG00000032282 RLG00000032283 RLG00000032308 RLG00000032413 RLG00000033129 RLG00000034945
rosa_multiflora Rmu_co8241929.1_g000001 Rmu_co8269227.1_g000001 Rmu_co8461259.1_g000001 Rmu_co8495385.1_g000001 Rmu_sc0000028.1_g000003 Rmu_sc0000083.1_g000009 Rmu_sc0000083.1_g000010 Rmu_sc0000083.1_g000031 Rmu_sc0000083.1_g000043 Rmu_sc0000083.1_g000046 Rmu_sc0000083.1_g000047 Rmu_sc0000563.1_g000024 Rmu_sc0000842.1_g000012 Rmu_sc0001235.1_g000001 Rmu_sc0001235.1_g000005 Rmu_sc0001235.1_g000009 Rmu_sc0001235.1_g000015 Rmu_sc0001235.1_g000016 Rmu_sc0001235.1_g000017 Rmu_sc0001473.1_g000036 Rmu_sc0001607.1_g000016 Rmu_sc0001788.1_g000006 Rmu_sc0002018.1_g000008 Rmu_sc0002028.1_g000001 Rmu_sc0002680.1_g000011 Rmu_sc0002798.1_g000023 Rmu_sc0002886.1_g000023 Rmu_sc0002989.1_g000012 Rmu_sc0003064.1_g000009 Rmu_sc0003382.1_g000006 Rmu_sc0003630.1_g000002 Rmu_sc0003630.1_g000047 Rmu_sc0003630.1_g000066 Rmu_sc0003630.1_g000067 Rmu_sc0003649.1_g000010 Rmu_sc0003688.1_g000005 Rmu_sc0003920.1_g000007 Rmu_sc0004765.1_g000007 Rmu_sc0004765.1_g000008 Rmu_sc0004920.1_g000011 Rmu_sc0005223.1_g000008 Rmu_sc0005223.1_g000010 Rmu_sc0005223.1_g000015 Rmu_sc0005223.1_g000016 Rmu_sc0005319.1_g000014 Rmu_sc0005319.1_g000015 Rmu_sc0005319.1_g000018 Rmu_sc0005319.1_g000019 Rmu_sc0005319.1_g000025 Rmu_sc0005418.1_g000007 Rmu_sc0005418.1_g000012 Rmu_sc0006106.1_g000011 Rmu_sc0006914.1_g000012 Rmu_sc0007023.1_g000001 Rmu_sc0007662.1_g000001 Rmu_sc0007920.1_g000002 Rmu_sc0007920.1_g000013 Rmu_sc0008053.1_g000016 Rmu_sc0008053.1_g000017 Rmu_sc0008053.1_g000018 Rmu_sc0008053.1_g000023 Rmu_sc0008053.1_g000050 Rmu_sc0009042.1_g000007 Rmu_sc0009042.1_g000008 Rmu_sc0010642.1_g000008 Rmu_sc0011109.1_g000002 Rmu_sc0011314.1_g000006 Rmu_sc0011974.1_g000006 Rmu_sc0012179.1_g000009 Rmu_sc0015169.1_g000001 Rmu_sc0016368.1_g000002 Rmu_sc0022659.1_g000001 Rmu_sc0027371.1_g000001 Rmu_sc0028213.1_g000001 Rmu_sc0034526.1_g000001 Rmu_sc0039366.1_g000002 Rmu_ssc0000050.1_g000084 Rmu_ssc0000050.1_g000088 Rmu_ssc0000167.1_g000017
rosa_roxburghii Rroxscaffold_1G00011340 Rroxscaffold_1G00025710 Rroxscaffold_1G00025870 Rroxscaffold_1G00026070 Rroxscaffold_1G00026170 Rroxscaffold_1G00026200 Rroxscaffold_1G00026270 Rroxscaffold_1G00050950 Rroxscaffold_1G00058900 Rroxscaffold_1G00060040 Rroxscaffold_1G00060060 Rroxscaffold_1G00060070 Rroxscaffold_1G00060080 Rroxscaffold_1G00060110 Rroxscaffold_1G00060120 Rroxscaffold_1G00060140 Rroxscaffold_1G00060170 Rroxscaffold_1G00060210 Rroxscaffold_1G00060220 Rroxscaffold_1G00060240 Rroxscaffold_1G00060250 Rroxscaffold_1G00060260 Rroxscaffold_1G00060300 Rroxscaffold_1G00060320 Rroxscaffold_1G00060330 Rroxscaffold_1G00060900 Rroxscaffold_1G00060920 Rroxscaffold_1G00073980 Rroxscaffold_2G00077560 Rroxscaffold_2G00077580 Rroxscaffold_2G00146990 Rroxscaffold_3G00236200 Rroxscaffold_3G00236210 Rroxscaffold_3G00236220 Rroxscaffold_4G00292760 Rroxscaffold_4G00315200 Rroxscaffold_4G00315240 Rroxscaffold_4G00326240 Rroxscaffold_4G00328980 Rroxscaffold_5G00341890 Rroxscaffold_7G00161350 Rroxscaffold_7G00174490 Rroxscaffold_7G00206550
rosa_rugosa Rorug01G0093000 Rorug01G0093100 Rorug01G0128800 Rorug01G0312000 Rorug01G0312000 Rorug02G0581100 Rorug03G0224600 Rorug05G0028500 Rorug05G0032700 Rorug05G0032800 Rorug05G0032900 Rorug05G0032900 Rorug05G0033000 Rorug05G0033100 Rorug05G0033300 Rorug05G0033400 Rorug05G0033900 Rorug05G0034900 Rorug05G0035000 Rorug05G0046900 Rorug05G0047500 Rorug05G0047500 Rorug05G0111600 Rorug05G0287900 Rorug05G0288700 Rorug05G0586900 Rorug06G0231700 Rorug06G0354300 Rorug06G0354400
rosa_samantha Rh1DG122200 Rh2DG686800 Rh5BG136600 Rh6AG344500 Rh6BG351900 Rh6CG359000 Rh6CG359100 Rh6CG387600 Rh6CG480500 Rh6DG344600 Rh6DG467400
rosa_wichuraiana Rw0G010250 Rw0G014820 Rw0G021400 Rw0G023100 Rw1G009450 Rw1G012100 Rw1G012180 Rw1G028360 Rw2G054260 Rw3G024370 Rw3G024420 Rw3G024470 Rw5G010850 Rw5G011010 Rw5G011020 Rw5G011050 Rw5G011080 Rw5G011120 Rw5G012300 Rw5G018470 Rw5G033830 Rw6G008930 Rw6G030070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1314
AarI CACCTGC 1 cut(s) 1310
Acc36I ACCTGC 2 cut(s) 174, 1310
AccB7I CCANNNNNTGG 1 cut(s) 1155
AccI GTMKAC 3 cut(s) 445, 849, 1530
AciI CCGC 6 cut(s) 117, 371, 1215, 1889, 2228, 2268
AclWI GGATC 3 cut(s) 192, 205, 1040
AcoI YGGCCR 1 cut(s) 1728
AcsI RAATTY 6 cut(s) 176, 1095, 1326, 1995, 2010, 2286
AcuI CTGAAG 4 cut(s) 434, 1305, 1533, 1634
AfiI CCNNNNNNNGG 4 cut(s) 470, 926, 1155, 2181
AflII CTTAAG 1 cut(s) 1292
AgeI ACCGGT 1 cut(s) 1544
AgsI TTSAA 9 cut(s) 181, 733, 960, 1064, 1140, 1573, 1839, 1971, 1990
AhlI ACTAGT 2 cut(s) 1525, 1792
AjiI CACGTC 2 cut(s) 213, 1690
AjnI CCWGG 4 cut(s) 97, 901, 951, 1000
AjuI GAANNNNNNNTTGG 4 cut(s) 26, 58, 1694, 1726
Alw26I GTCTC 5 cut(s) 1112, 1256, 1502, 1680, 2027
AlwI GGATC 3 cut(s) 192, 205, 1040
Ama87I CYCGRG 1 cut(s) 1931
AoxI GGCC 5 cut(s) 3, 300, 1728, 1928, 2093
ApeKI GCWGC 9 cut(s) 59, 77, 80, 159, 367, 428, 488, 666, 745
ApoI RAATTY 6 cut(s) 176, 1095, 1326, 1995, 2010, 2286
AsiGI ACCGGT 1 cut(s) 1544
Asp700I GAANNNNTTC 3 cut(s) 640, 1009, 1555
AspS9I GGNCC 2 cut(s) 1819, 1929
AsuC2I CCSGG 2 cut(s) 493, 710
AsuHPI GGTGA 8 cut(s) 131, 293, 987, 1334, 1346, 1459, 1534, 2252
AsuII TTCGAA 1 cut(s) 2213
AsuNHI GCTAGC 2 cut(s) 1237, 1885
AvaI CYCGRG 1 cut(s) 1931
AvaII GGWCC 1 cut(s) 1819
BaeGI GKGCMC 2 cut(s) 568, 1642
BaeI ACNNNNGTAYC 2 cut(s) 841, 874
BamHI GGATCC 1 cut(s) 197
BarI GAAGNNNNNNTAC 2 cut(s) 1506, 1538
BauI CACGAG 1 cut(s) 539
BbsI GAAGAC 1 cut(s) 2222
BbvI GCAGC 9 cut(s) 71, 89, 92, 146, 354, 440, 500, 678, 757
BccI CCATC 5 cut(s) 149, 392, 584, 869, 1400
BceAI ACGGC 6 cut(s) 287, 568, 1250, 1296, 1647, 1743
BcgI CGANNNNNNTGC 2 cut(s) 922, 956
BciT130I CCWGG 4 cut(s) 99, 903, 953, 1002
BciVI GTATCC 2 cut(s) 1805, 2224
BclI TGATCA 2 cut(s) 1429, 1561
BcnI CCSGG 2 cut(s) 493, 710
BcoDI GTCTC 5 cut(s) 1112, 1256, 1502, 1680, 2027
BcuI ACTAGT 2 cut(s) 1525, 1792
BfaI CTAG 8 cut(s) 311, 1238, 1526, 1793, 1886, 1926, 1949, 2253
BfmI CTRYAG 1 cut(s) 1767
BfrI CTTAAG 1 cut(s) 1292
BfuAI ACCTGC 2 cut(s) 174, 1310
BfuI GTATCC 2 cut(s) 1805, 2224
BglII AGATCT 1 cut(s) 1586
BmcAI AGTACT 3 cut(s) 759, 817, 1199
Bme1390I CCNGG 6 cut(s) 99, 493, 710, 903, 953, 1002
Bme18I GGWCC 1 cut(s) 1819
BmeT110I CYCGRG 1 cut(s) 1931
BmgBI CACGTC 2 cut(s) 213, 1690
BmgT120I GGNCC 2 cut(s) 1819, 1929
BmiI GGNNCC 3 cut(s) 199, 1414, 1821
BmrFI CCNGG 6 cut(s) 99, 493, 710, 903, 953, 1002
BmrI ACTGGG 2 cut(s) 778, 1418
BmsI GCATC 4 cut(s) 12, 604, 1631, 1993
BmtI GCTAGC 2 cut(s) 1241, 1889
BmuI ACTGGG 2 cut(s) 778, 1418
BpiI GAAGAC 1 cut(s) 2222
BpmI CTGGAG 3 cut(s) 37, 383, 984
Bpu14I TTCGAA 1 cut(s) 2213
BpuEI CTTGAG 1 cut(s) 1764
BpuMI CCSGG 2 cut(s) 493, 710
Bsa29I ATCGAT 1 cut(s) 942
BsaI GGTCTC 1 cut(s) 1112
BsaJI CCNNGG 6 cut(s) 609, 708, 910, 925, 1932, 2153
BsaWI WCCGGW 1 cut(s) 1544
BsaXI ACNNNNNCTCC 6 cut(s) 1242, 1272, 1640, 1654, 1670, 1684
Bsc4I CCNNNNNNNGG 4 cut(s) 470, 926, 1155, 2181
Bse118I RCCGGY 1 cut(s) 1544
Bse1I ACTGG 5 cut(s) 20, 755, 773, 1413, 2277
BseBI CCWGG 4 cut(s) 99, 903, 953, 1002
BseCI ATCGAT 1 cut(s) 942
BseDI CCNNGG 6 cut(s) 609, 708, 910, 925, 1932, 2153
BseGI GGATG 7 cut(s) 27, 576, 619, 1145, 2136, 2149, 2204
BseLI CCNNNNNNNGG 4 cut(s) 470, 926, 1155, 2181
BseMII CTCAG 3 cut(s) 267, 2169, 2178
BseNI ACTGG 5 cut(s) 20, 755, 773, 1413, 2277
BseRI GAGGAG 4 cut(s) 1266, 1269, 1648, 2164
BseSI GKGCMC 2 cut(s) 568, 1642
BseXI GCAGC 9 cut(s) 71, 89, 92, 146, 354, 440, 500, 678, 757
BsgI GTGCAG 2 cut(s) 126, 685
Bsh1285I CGRYCG 1 cut(s) 853
BshFI GGCC 5 cut(s) 5, 302, 1730, 1930, 2095
BshTI ACCGGT 1 cut(s) 1544
BshVI ATCGAT 1 cut(s) 942
BsiEI CGRYCG 1 cut(s) 853
BsiHKCI CYCGRG 1 cut(s) 1931
BsiSI CCGG 3 cut(s) 492, 710, 1545
BslFI GGGAC 3 cut(s) 794, 1816, 2197
BslI CCNNNNNNNGG 4 cut(s) 470, 926, 1155, 2181
BsmAI GTCTC 5 cut(s) 1112, 1256, 1502, 1680, 2027
BsmBI CGTCTC 1 cut(s) 1680
BsmFI GGGAC 3 cut(s) 794, 1816, 2197
BsmI GAATGC 2 cut(s) 674, 1988
BsnI GGCC 5 cut(s) 5, 302, 1730, 1930, 2095
Bso31I GGTCTC 1 cut(s) 1112
BsoBI CYCGRG 1 cut(s) 1931
Bsp119I TTCGAA 1 cut(s) 2213
Bsp1286I GDGCHC 2 cut(s) 568, 1642
Bsp143I GATC 7 cut(s) 85, 197, 1045, 1429, 1561, 1586, 1771
Bsp19I CCATGG 2 cut(s) 910, 2153
BspACI CCGC 6 cut(s) 117, 371, 1215, 1889, 2228, 2268
BspANI GGCC 5 cut(s) 5, 302, 1730, 1930, 2095
BspCNI CTCAG 3 cut(s) 268, 2170, 2179
BspDI ATCGAT 1 cut(s) 942
BspLI GGNNCC 3 cut(s) 199, 1414, 1821
BspMI ACCTGC 2 cut(s) 174, 1310
BspOI GCTAGC 2 cut(s) 1241, 1889
BspPI GGATC 3 cut(s) 192, 205, 1040
BspQI GCTCTTC 1 cut(s) 1281
BspT104I TTCGAA 1 cut(s) 2213
BspTI CTTAAG 1 cut(s) 1292
BspTNI GGTCTC 1 cut(s) 1112
BsrFI RCCGGY 1 cut(s) 1544
BsrI ACTGG 5 cut(s) 20, 755, 773, 1413, 2277
BssAI RCCGGY 1 cut(s) 1544
BssECI CCNNGG 6 cut(s) 609, 708, 910, 925, 1932, 2153
BssMI GATC 7 cut(s) 85, 197, 1045, 1429, 1561, 1586, 1771
BssNAI GTATAC 1 cut(s) 1531
BssSI CACGAG 1 cut(s) 539
BssT1I CCWWGG 4 cut(s) 609, 910, 925, 2153
Bst1107I GTATAC 1 cut(s) 1531
Bst2BI CACGAG 1 cut(s) 539
Bst2UI CCWGG 4 cut(s) 99, 903, 953, 1002
Bst4CI ACNGT 6 cut(s) 233, 502, 604, 815, 877, 1354
Bst6I CTCTTC 2 cut(s) 1281, 1932
BstAFI CTTAAG 1 cut(s) 1292
BstAPI GCANNNNNTGC 1 cut(s) 295
BstBI TTCGAA 1 cut(s) 2213
BstC8I GCNNGC 5 cut(s) 300, 826, 899, 1239, 1887
BstDEI CTNAG 4 cut(s) 276, 581, 2178, 2187
BstDSI CCRYGG 2 cut(s) 910, 2153
BstEII GGTNACC 2 cut(s) 881, 905
BstF5I GGATG 7 cut(s) 27, 576, 619, 1145, 2136, 2149, 2204
BstKTI GATC 7 cut(s) 88, 200, 1048, 1432, 1564, 1589, 1774
BstMAI GTCTC 5 cut(s) 1112, 1256, 1502, 1680, 2027
BstMBI GATC 7 cut(s) 85, 197, 1045, 1429, 1561, 1586, 1771
BstMCI CGRYCG 1 cut(s) 853
BstMWI GCNNNNNNNGC 7 cut(s) 89, 159, 295, 367, 1183, 1891, 2057
BstNI CCWGG 4 cut(s) 99, 903, 953, 1002
BstNSI RCATGY 1 cut(s) 828
BstPI GGTNACC 2 cut(s) 881, 905
BstSCI CCNGG 6 cut(s) 97, 491, 708, 901, 951, 1000
BstSFI CTRYAG 1 cut(s) 1767
BstSLI GKGCMC 2 cut(s) 568, 1642
BstV1I GCAGC 9 cut(s) 71, 89, 92, 146, 354, 440, 500, 678, 757
BstV2I GAAGAC 1 cut(s) 2222
BstX2I RGATCY 3 cut(s) 197, 1045, 1586
BstYI RGATCY 3 cut(s) 197, 1045, 1586
BstZ17I GTATAC 1 cut(s) 1531
Bsu15I ATCGAT 1 cut(s) 942
BsuI GTATCC 2 cut(s) 1805, 2224
BsuRI GGCC 5 cut(s) 5, 302, 1730, 1930, 2095
BsuTUI ATCGAT 1 cut(s) 942
BtgI CCRYGG 2 cut(s) 910, 2153
BtgZI GCGATG 1 cut(s) 168
BtrI CACGTC 2 cut(s) 213, 1690
BtsCI GGATG 7 cut(s) 27, 576, 619, 1145, 2136, 2149, 2204
BtsI GCAGTG 1 cut(s) 892
BtsIMutI CAGTG 3 cut(s) 13, 498, 892
BveI ACCTGC 2 cut(s) 174, 1310
Cac8I GCNNGC 5 cut(s) 300, 826, 899, 1239, 1887
Cfr10I RCCGGY 1 cut(s) 1544
Cfr13I GGNCC 2 cut(s) 1819, 1929
ClaI ATCGAT 1 cut(s) 942
CseI GACGC 1 cut(s) 158
CsiI ACCWGGT 1 cut(s) 97
CspAI ACCGGT 1 cut(s) 1544
DdeI CTNAG 4 cut(s) 276, 581, 2178, 2187
DpnI GATC 7 cut(s) 87, 199, 1047, 1431, 1563, 1588, 1773
DpnII GATC 7 cut(s) 85, 197, 1045, 1429, 1561, 1586, 1771
DraI TTTAAA 1 cut(s) 1204
EaeI YGGCCR 1 cut(s) 1728
Eam1104I CTCTTC 2 cut(s) 1281, 1932
EarI CTCTTC 2 cut(s) 1281, 1932
Eco130I CCWWGG 4 cut(s) 609, 910, 925, 2153
Eco147I AGGCCT 1 cut(s) 2095
Eco31I GGTCTC 1 cut(s) 1112
Eco32I GATATC 1 cut(s) 940
Eco47I GGWCC 1 cut(s) 1819
Eco57I CTGAAG 4 cut(s) 434, 1305, 1533, 1634
Eco88I CYCGRG 1 cut(s) 1931
Eco91I GGTNACC 2 cut(s) 881, 905
EcoO65I GGTNACC 2 cut(s) 881, 905
EcoRI GAATTC 1 cut(s) 176
EcoRII CCWGG 4 cut(s) 97, 901, 951, 1000
EcoRV GATATC 1 cut(s) 940
EcoT14I CCWWGG 4 cut(s) 609, 910, 925, 2153
EcoT22I ATGCAT 1 cut(s) 826
ErhI CCWWGG 4 cut(s) 609, 910, 925, 2153
Esp3I CGTCTC 1 cut(s) 1680
FalI AAGNNNNNCTT 4 cut(s) 432, 464, 1791, 1823
FaqI GGGAC 3 cut(s) 794, 1816, 2197
FauI CCCGC 1 cut(s) 2261
FbaI TGATCA 2 cut(s) 1429, 1561
FblI GTMKAC 3 cut(s) 445, 849, 1530
FokI GGATG 7 cut(s) 34, 563, 626, 1132, 2136, 2143, 2191
FspBI CTAG 8 cut(s) 311, 1238, 1526, 1793, 1886, 1926, 1949, 2253
GsuI CTGGAG 3 cut(s) 37, 383, 984
HaeIII GGCC 5 cut(s) 5, 302, 1730, 1930, 2095
HapII CCGG 3 cut(s) 492, 710, 1545
HgaI GACGC 1 cut(s) 158
HincII GTYRAC 2 cut(s) 216, 850
HindII GTYRAC 2 cut(s) 216, 850
HinfI GANTC 7 cut(s) 542, 588, 623, 641, 1119, 1990, 2053
HpaII CCGG 3 cut(s) 492, 710, 1545
HphI GGTGA 8 cut(s) 131, 293, 987, 1334, 1346, 1459, 1534, 2252
Hpy166II GTNNAC 8 cut(s) 216, 446, 498, 587, 685, 850, 1357, 1531
Hpy188I TCNGA 8 cut(s) 277, 628, 1561, 1627, 1741, 2016, 2179, 2292
Hpy188III TCNNGA 4 cut(s) 173, 1573, 1685, 2000
Hpy8I GTNNAC 8 cut(s) 216, 446, 498, 587, 685, 850, 1357, 1531
HpyAV CCTTC 6 cut(s) 434, 464, 683, 1167, 1291, 1331
HpyCH4III ACNGT 6 cut(s) 233, 502, 604, 815, 877, 1354
HpyCH4IV ACGT 4 cut(s) 212, 1479, 1689, 1756
HpyF10VI GCNNNNNNNGC 7 cut(s) 89, 159, 295, 367, 1183, 1891, 2057
HpyF3I CTNAG 4 cut(s) 276, 581, 2178, 2187
HpySE526I ACGT 4 cut(s) 212, 1479, 1689, 1756
Ksp22I TGATCA 2 cut(s) 1429, 1561
Kzo9I GATC 7 cut(s) 85, 197, 1045, 1429, 1561, 1586, 1771
LguI GCTCTTC 1 cut(s) 1281
LmnI GCTCC 4 cut(s) 364, 648, 1748, 1891
Lsp1109I GCAGC 9 cut(s) 71, 89, 92, 146, 354, 440, 500, 678, 757
LweI GCATC 4 cut(s) 12, 604, 1631, 1993
MabI ACCWGGT 1 cut(s) 97
MaeI CTAG 8 cut(s) 311, 1238, 1526, 1793, 1886, 1926, 1949, 2253
MaeII ACGT 4 cut(s) 212, 1479, 1689, 1756
MaeIII GTNAC 8 cut(s) 64, 476, 502, 548, 881, 905, 1022, 2301
MalI GATC 7 cut(s) 87, 199, 1047, 1431, 1563, 1588, 1773
MboI GATC 7 cut(s) 85, 197, 1045, 1429, 1561, 1586, 1771
MboII GAAGA 6 cut(s) 817, 1298, 1627, 1949, 1959, 2227
MfeI CAATTG 2 cut(s) 187, 728
MflI RGATCY 3 cut(s) 197, 1045, 1586
MhlI GDGCHC 2 cut(s) 568, 1642
MlyI GAGTC 2 cut(s) 551, 582
MmeI TCCRAC 2 cut(s) 748, 2103
Mph1103I ATGCAT 1 cut(s) 826
MroXI GAANNNNTTC 3 cut(s) 640, 1009, 1555
MseI TTAA 5 cut(s) 653, 765, 1203, 1293, 2297
MslI CAYNNNNRTG 2 cut(s) 2158, 2310
MspA1I CMGCKG 2 cut(s) 62, 2268
MspCI CTTAAG 1 cut(s) 1292
MspI CCGG 3 cut(s) 492, 710, 1545
MspR9I CCNGG 6 cut(s) 99, 493, 710, 903, 953, 1002
MunI CAATTG 2 cut(s) 187, 728
Mva1269I GAATGC 2 cut(s) 674, 1988
MvaI CCWGG 4 cut(s) 99, 903, 953, 1002
MwoI GCNNNNNNNGC 7 cut(s) 89, 159, 295, 367, 1183, 1891, 2057
NciI CCSGG 2 cut(s) 493, 710
NcoI CCATGG 2 cut(s) 910, 2153
NdeII GATC 7 cut(s) 85, 197, 1045, 1429, 1561, 1586, 1771
NheI GCTAGC 2 cut(s) 1237, 1885
NlaIV GGNNCC 3 cut(s) 199, 1414, 1821
NmuCI GTSAC 4 cut(s) 476, 502, 548, 2301
NsiI ATGCAT 1 cut(s) 826
NspI RCATGY 1 cut(s) 828
NspV TTCGAA 1 cut(s) 2213
PaeI GCATGC 1 cut(s) 828
PaqCI CACCTGC 1 cut(s) 1310
PceI AGGCCT 1 cut(s) 2095
PciSI GCTCTTC 1 cut(s) 1281
PctI GAATGC 2 cut(s) 674, 1988
PdmI GAANNNNTTC 3 cut(s) 640, 1009, 1555
PfeI GAWTC 5 cut(s) 623, 641, 1119, 1990, 2053
PflMI CCANNNNNTGG 1 cut(s) 1155
PfoI TCCNGGA 1 cut(s) 1000
PinAI ACCGGT 1 cut(s) 1544
PleI GAGTC 2 cut(s) 550, 582
PpsI GAGTC 2 cut(s) 550, 582
PsiI TTATAA 1 cut(s) 1314
Psp6I CCWGG 4 cut(s) 97, 901, 951, 1000
PspEI GGTNACC 2 cut(s) 881, 905
PspGI CCWGG 4 cut(s) 97, 901, 951, 1000
PspN4I GGNNCC 3 cut(s) 199, 1414, 1821
PspPI GGNCC 2 cut(s) 1819, 1929
PsuI RGATCY 3 cut(s) 197, 1045, 1586
PvuII CAGCTG 1 cut(s) 62
RseI CAYNNNNRTG 2 cut(s) 2158, 2310
SalI GTCGAC 1 cut(s) 848
SapI GCTCTTC 1 cut(s) 1281
SaqAI TTAA 5 cut(s) 653, 765, 1203, 1293, 2297
Sau3AI GATC 7 cut(s) 85, 197, 1045, 1429, 1561, 1586, 1771
Sau96I GGNCC 2 cut(s) 1819, 1929
ScaI AGTACT 3 cut(s) 759, 817, 1199
SchI GAGTC 2 cut(s) 551, 582
ScrFI CCNGG 6 cut(s) 99, 493, 710, 903, 953, 1002
SduI GDGCHC 2 cut(s) 568, 1642
SexAI ACCWGGT 1 cut(s) 97
SfaNI GCATC 4 cut(s) 12, 604, 1631, 1993
SfcI CTRYAG 1 cut(s) 1767
SfuI TTCGAA 1 cut(s) 2213
SgrAI CRCCGGYG 1 cut(s) 1544
SinI GGWCC 1 cut(s) 1819
SmiMI CAYNNNNRTG 2 cut(s) 2158, 2310
SmlI CTYRAG 2 cut(s) 1292, 1779
SmoI CTYRAG 2 cut(s) 1292, 1779
SpeI ACTAGT 2 cut(s) 1525, 1792
SphI GCATGC 1 cut(s) 828
SseBI AGGCCT 1 cut(s) 2095
SsiI CCGC 6 cut(s) 117, 371, 1215, 1889, 2228, 2268
SspI AATATT 1 cut(s) 1368
SspMI CTAG 8 cut(s) 311, 1238, 1526, 1793, 1886, 1926, 1949, 2253
StuI AGGCCT 1 cut(s) 2095
StyD4I CCNGG 6 cut(s) 97, 491, 708, 901, 951, 1000
StyI CCWWGG 4 cut(s) 609, 910, 925, 2153
TaaI ACNGT 6 cut(s) 233, 502, 604, 815, 877, 1354
TaiI ACGT 4 cut(s) 215, 1482, 1692, 1759
TaqI TCGA 4 cut(s) 849, 942, 1684, 2213
TaqII GACCGA 2 cut(s) 867, 1094
TatI WGTACW 4 cut(s) 757, 815, 1197, 1536
TauI GCSGC 2 cut(s) 373, 1218
TfiI GAWTC 5 cut(s) 623, 641, 1119, 1990, 2053
Tru1I TTAA 5 cut(s) 653, 765, 1203, 1293, 2297
Tru9I TTAA 5 cut(s) 653, 765, 1203, 1293, 2297
TscAI CASTG 3 cut(s) 20, 505, 899
TseFI GTSAC 4 cut(s) 476, 502, 548, 2301
TseI GCWGC 9 cut(s) 59, 77, 80, 159, 367, 428, 488, 666, 745
Tsp45I GTSAC 4 cut(s) 476, 502, 548, 2301
TspDTI ATGAA 9 cut(s) 453, 483, 667, 1089, 1178, 1587, 1750, 2117, 2322
TspGWI ACGGA 4 cut(s) 909, 1041, 1362, 2132
TspRI CASTG 3 cut(s) 20, 505, 899
Van91I CCANNNNNTGG 1 cut(s) 1155
Vha464I CTTAAG 1 cut(s) 1292
VpaK11BI GGWCC 1 cut(s) 1819
XapI RAATTY 6 cut(s) 176, 1095, 1326, 1995, 2010, 2286
XceI RCATGY 1 cut(s) 828
XmiI GTMKAC 3 cut(s) 445, 849, 1530
XmnI GAANNNNTTC 3 cut(s) 640, 1009, 1555
XspI CTAG 8 cut(s) 311, 1238, 1526, 1793, 1886, 1926, 1949, 2253
ZrmI AGTACT 3 cut(s) 759, 817, 1199
Zsp2I ATGCAT 1 cut(s) 826
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.