Rroxscaffold_1G00060170

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000066
Physical Location & Seq
Forward (+)
82313818 .. 82318129
4312 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00060170.1

Sequence Viewer

Length: 2334 bp
ATGGCCTCATTACATGAGAGGATTCTTGTTACGCTGCTCTATTTGTTAATGTTAGTGGCCGCAACAACAACATTGCTAGCAGCCGCGCAGACCCTAGTGCCGCCTCCTCAAGCCAAGCCAGGATGCCAAGAAAAGTGCGGTGATCTCACAATCCCATACCCGTTTGGCCTAGGCGAAGCTTGTTCCATGCGACCCGAGTTCACCATCACTTGCGATCAATCCACCACAGCACCCCCATCAGCAAACTTTACAAACAGTACCTTCACCTTTCCCATCACCATTACTAACTTCTCCCTTGTTGACGGTGAGTTACAAGTAATGCAGGACATAGCCAGAAACTGCTATGACGAGGAGGGCAATAATGCAAACAGGAGCAGTTCACTCGAGCTGCCTCCTCCTTACACCATCTCAACCAAAAACAACTTCTTTACCCTTGGATGTAACGTTTTAGCAGTCTACCAGGAAGGTGTGGATGTAGACTACCAAATCGGAGTAAATGTGGCCTTATGTATGGATATTCTTGGCAAGGAATTGACCGAGTCTTGCATCGGCGTTGGGTGCGCCCAAACTTCCATCCCTAGTGGACTGCAAAACATTTCGATTGGAGTGGCTTCACTTAACGGGTATAACGCGAGTGATCCCTGGCAATCAAACTACAAGTGCAGCTACGCTTTCATCGTTGAAGAAGGCAACTTCACATTCGCACCGAAAACGAGTTTTCAACAACTGAATACAACAAATCATCAGCTTCCGGTGGCTGTTAATTGGGCAATCGGGGACGAGTCGTGTGCAGTAGCGAAGAACCTAACTGATTATTCGTGCAAAGAGAATACTATTTGTGTCGACCGGAGCACCATCACTAATGAGACGGCTGCAGGTTACATTTGCCAGTGCTTGCCAGGCTACGAAGGGAACCCATACCTCGGTTGTCGAGATATCGATGAGTGCACGACCTCATCAAGGGACCCCTGCACGAATGGAAAGTGTGTAAATTCACCTCCAGGGAATTACTCGTGTAAATGTAATAAAGGGTTCAGAAACGAAGACGACATGACTTGCATCCCATATTCCTGGTCAAACAACACTTCCCTAAAAATATCATTAGGAGTGAGTATAGGCTTCTTGGTTTTAGTGGTCTTAATTTCGTGGATGTATTGGGAAATGAACAAAAGGAGGCTCATCACACTCAAAAAGAAGTACTTCAAAGAAAACGGTGGCTTAACATTACAACAACAACTCGCTAGTCATGGAGGAGCCGTGGAAAAAACAAAAATCTTTACAGCGGAAGAACTTGAGAAGGCAACAAACAATTACCATGCAGATGAGATTCTTGGTGAAGGAGCCTATGGAACAGTTTACAAAGGAATTCTACCTGATAAGAAAGTTGTTGCCATAAAAAAATCAAAAATTGGCGCCCCAACTCAAAGTGAGCAGTTCGTTAATGAGGTTATCGTTCTCTCTCAAATCAACCACAGAAATGTAGTAAAACTTTTGGGTTGTTGTTTCGAGACTGAAGTGCCTTTACTAGTTTATGAGTATATTACCCATGGCACTCTTTTTGAGCATATATTCAATAAGGGAAAAGGATCACCGCTTTCATGGGAATTACGATTGAAGATAGCATCAGAAACGGCAGGGGCACTAGCTTACTTACACTCCTCTACTTCCACACCAATCATACATCGAGACGTGAAAGCAACAAATATACTACTAGATGACAATTACACGGCCAAAGTGTCCGATTTTGGAGCTTCGCGTTTCATTCCTCTAGATCAAACTGAACTAGCAACACTAGTGCAAGGGACGCTTGGCTACTTGGACCCTGAATACTTTCATTCCAATCAACTGACTGAAAAGAGTGACGTCTACAGCTTTGGAGTTGTCCTAGCAGAGTTATTAACGAGCAGAGTGGCACTTTCTTTCGCTAGACCTGACGCAGAGAGATGCCTAGCAAGTTTCTTTGTTTCTTCAATAGAGAAAGATTGCTTGATTCAAATTCTTGACGCTGACATAGTGAATGAGGGGAACATAGACACAGCAGAGCAAGTTGCTTATCTCGCAAAAAGATGTCTAGGGCTAAGAGGGGATGAAAGGCCTTCAATGAAAGAAGTGGCCATGGAGTTGGAGGGAATGCAAAATACAGCGAAGCATCCATGGGGAAATAATGCAAATTCATGTCCCGAGGAGACTGAGTACTTGCTGGGGTCACCTATCAATTCAGAAGCTTACATTGTGAATGTTAAAGGTGATGGTGATGAAGGTTCTAGTGGTACAGCTAGTGGGTATGACAGCATGCAAATTCAAATGTTAATGCCATATGATGATCCCCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK hEGF

Protein Analysis

777

Amino Acids

85.15

Weight (kDa)

4.85

Isoelectric Point (pI)

35.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 42 - 105 3e-09 Wall-associated receptor kinase galacturonan-binding
Pkinase PF00069 438 - 704 2.9e-45 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 440 - 708 5e-46 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21210 AT1G21230 AT1G21240 AT1G21240 AT1G21245 AT1G21250 AT1G21270 AT1G22720 AT3G25490
fragaria_vesca FvH4_2g35051 FvH4_2g35051 FvH4_2g35060 FvH4_3g10281 FvH4_3g10285 FvH4_3g10286 FvH4_3g10311 FvH4_3g10311 FvH4_3g10320 FvH4_3g10332 FvH4_3g10340 FvH4_3g10350 FvH4_3g10360 FvH4_3g10390 FvH4_3g10412 FvH4_3g10413 FvH4_3g10461 FvH4_3g10462 FvH4_3g10470 FvH4_3g10510 FvH4_3g10520 FvH4_3g10530 FvH4_3g10550 FvH4_3g10560 FvH4_3g10611 FvH4_3g11730 FvH4_6g53140 FvH4_6g53142 FvH4_7g31880 FvH4_7g31910 FvH4_7g31920
malus_domestica MD05G1269900.v1.1 MD05G1270000.v1.1 MD05G1270200.v1.1 MD05G1270400.v1.1 MD05G1270500.v1.1 MD05G1270600.v1.1 MD05G1270800.v1.1 MD05G1270900.v1.1 MD07G1183800.v1.1 MD08G1107600.v1.1 MD10G1248500.v1.1 MD10G1249200.v1.1 MD10G1249500.v1.1 MD10G1250000.v1.1 MD10G1250500.v1.1 MD10G1250900.v1.1 MD10G1251200.v1.1 MD10G1251400.v1.1 MD13G1004600.v1.1 MD15G1088400.v1.1
prunus_persica Prupe.1G442400_v2.0.a1 Prupe.1G442500_v2.0.a1 Prupe.2G223400_v2.0.a1 Prupe.4G091900_v2.0.a1 Prupe.4G093200_v2.0.a1 Prupe.4G093300_v2.0.a1 Prupe.4G093400_v2.0.a1 Prupe.4G093500_v2.0.a1 Prupe.4G094100_v2.0.a1 Prupe.4G094200_v2.0.a1 Prupe.4G094300_v2.0.a1 Prupe.6G358300_v2.0.a1
pyrus_communis pycom08g08990 pycom10g20660 pycom10g20670 pycom10g20680 pycom10g20710 pycom10g20740 pycom13g00400 pycom15g08290
rosa_chinensis RchiOBHm_Chr1g0326861 RchiOBHm_Chr1g0326871 RchiOBHm_Chr1g0331391 RchiOBHm_Chr1g0336911 RchiOBHm_Chr1g0364001 RchiOBHm_Chr2g0174541 RchiOBHm_Chr2g0175041 RchiOBHm_Chr2g0175101 RchiOBHm_Chr2g0175121 RchiOBHm_Chr3g0486331 RchiOBHm_Chr5g0015581 RchiOBHm_Chr5g0016551 RchiOBHm_Chr5g0016561 RchiOBHm_Chr5g0016581 RchiOBHm_Chr5g0016601 RchiOBHm_Chr5g0016611 RchiOBHm_Chr5g0016641 RchiOBHm_Chr5g0016661 RchiOBHm_Chr5g0016691 RchiOBHm_Chr5g0016701 RchiOBHm_Chr5g0016731 RchiOBHm_Chr5g0016741 RchiOBHm_Chr5g0016811 RchiOBHm_Chr5g0016841 RchiOBHm_Chr5g0016871 RchiOBHm_Chr5g0016881 RchiOBHm_Chr5g0016931 RchiOBHm_Chr5g0017021 RchiOBHm_Chr5g0017041 RchiOBHm_Chr5g0017071 RchiOBHm_Chr5g0017091 RchiOBHm_Chr5g0017121 RchiOBHm_Chr5g0017141 RchiOBHm_Chr5g0017291 RchiOBHm_Chr5g0017321 RchiOBHm_Chr5g0017401 RchiOBHm_Chr5g0019001 RchiOBHm_Chr5g0019071 RchiOBHm_Chr5g0028941 RchiOBHm_Chr5g0052141 RchiOBHm_Chr5g0054721 RchiOBHm_Chr5g0054801 RchiOBHm_Chr5g0054931 RchiOBHm_Chr5g0054941 RchiOBHm_Chr6g0259291 RchiOBHm_Chr6g0292991 RchiOBHm_Chr6g0306911
rosa_laevigata RLG00000009465 RLG00000009468 RLG00000010755 RLG00000011976 RLG00000014541 RLG00000014542 RLG00000015169 RLG00000022308 RLG00000027529 RLG00000029324 RLG00000030244 RLG00000030245 RLG00000032210 RLG00000032211 RLG00000032257 RLG00000032259 RLG00000032260 RLG00000032261 RLG00000032262 RLG00000032268 RLG00000032269 RLG00000032272 RLG00000032282 RLG00000032283 RLG00000032308 RLG00000032413 RLG00000033129 RLG00000034945
rosa_multiflora Rmu_co8241929.1_g000001 Rmu_co8269227.1_g000001 Rmu_co8461259.1_g000001 Rmu_co8495385.1_g000001 Rmu_sc0000028.1_g000003 Rmu_sc0000083.1_g000009 Rmu_sc0000083.1_g000010 Rmu_sc0000083.1_g000031 Rmu_sc0000083.1_g000043 Rmu_sc0000083.1_g000046 Rmu_sc0000083.1_g000047 Rmu_sc0000563.1_g000024 Rmu_sc0000842.1_g000012 Rmu_sc0001235.1_g000001 Rmu_sc0001235.1_g000005 Rmu_sc0001235.1_g000009 Rmu_sc0001235.1_g000015 Rmu_sc0001235.1_g000016 Rmu_sc0001235.1_g000017 Rmu_sc0001473.1_g000036 Rmu_sc0001607.1_g000016 Rmu_sc0001788.1_g000006 Rmu_sc0002018.1_g000008 Rmu_sc0002028.1_g000001 Rmu_sc0002680.1_g000011 Rmu_sc0002798.1_g000023 Rmu_sc0002886.1_g000023 Rmu_sc0002989.1_g000012 Rmu_sc0003064.1_g000009 Rmu_sc0003382.1_g000006 Rmu_sc0003630.1_g000002 Rmu_sc0003630.1_g000047 Rmu_sc0003630.1_g000066 Rmu_sc0003630.1_g000067 Rmu_sc0003649.1_g000010 Rmu_sc0003688.1_g000005 Rmu_sc0003920.1_g000007 Rmu_sc0004765.1_g000007 Rmu_sc0004765.1_g000008 Rmu_sc0004920.1_g000011 Rmu_sc0005223.1_g000008 Rmu_sc0005223.1_g000010 Rmu_sc0005223.1_g000015 Rmu_sc0005223.1_g000016 Rmu_sc0005319.1_g000014 Rmu_sc0005319.1_g000015 Rmu_sc0005319.1_g000018 Rmu_sc0005319.1_g000019 Rmu_sc0005319.1_g000025 Rmu_sc0005418.1_g000007 Rmu_sc0005418.1_g000012 Rmu_sc0006106.1_g000011 Rmu_sc0006914.1_g000012 Rmu_sc0007023.1_g000001 Rmu_sc0007662.1_g000001 Rmu_sc0007920.1_g000002 Rmu_sc0007920.1_g000013 Rmu_sc0008053.1_g000016 Rmu_sc0008053.1_g000017 Rmu_sc0008053.1_g000018 Rmu_sc0008053.1_g000023 Rmu_sc0008053.1_g000050 Rmu_sc0009042.1_g000007 Rmu_sc0009042.1_g000008 Rmu_sc0010642.1_g000008 Rmu_sc0011109.1_g000002 Rmu_sc0011314.1_g000006 Rmu_sc0011974.1_g000006 Rmu_sc0012179.1_g000009 Rmu_sc0015169.1_g000001 Rmu_sc0016368.1_g000002 Rmu_sc0022659.1_g000001 Rmu_sc0027371.1_g000001 Rmu_sc0028213.1_g000001 Rmu_sc0034526.1_g000001 Rmu_sc0039366.1_g000002 Rmu_ssc0000050.1_g000084 Rmu_ssc0000050.1_g000088 Rmu_ssc0000167.1_g000017
rosa_roxburghii Rroxscaffold_1G00011340 Rroxscaffold_1G00025710 Rroxscaffold_1G00025870 Rroxscaffold_1G00026070 Rroxscaffold_1G00026170 Rroxscaffold_1G00026200 Rroxscaffold_1G00026270 Rroxscaffold_1G00050950 Rroxscaffold_1G00058900 Rroxscaffold_1G00060040 Rroxscaffold_1G00060060 Rroxscaffold_1G00060070 Rroxscaffold_1G00060080 Rroxscaffold_1G00060110 Rroxscaffold_1G00060120 Rroxscaffold_1G00060140 Rroxscaffold_1G00060170 Rroxscaffold_1G00060210 Rroxscaffold_1G00060220 Rroxscaffold_1G00060240 Rroxscaffold_1G00060250 Rroxscaffold_1G00060260 Rroxscaffold_1G00060300 Rroxscaffold_1G00060320 Rroxscaffold_1G00060330 Rroxscaffold_1G00060900 Rroxscaffold_1G00060920 Rroxscaffold_1G00073980 Rroxscaffold_2G00077560 Rroxscaffold_2G00077580 Rroxscaffold_2G00146990 Rroxscaffold_3G00236200 Rroxscaffold_3G00236210 Rroxscaffold_3G00236220 Rroxscaffold_4G00292760 Rroxscaffold_4G00315200 Rroxscaffold_4G00315240 Rroxscaffold_4G00326240 Rroxscaffold_4G00328980 Rroxscaffold_5G00341890 Rroxscaffold_7G00161350 Rroxscaffold_7G00174490 Rroxscaffold_7G00206550
rosa_rugosa Rorug01G0093000 Rorug01G0093100 Rorug01G0128800 Rorug01G0312000 Rorug01G0312000 Rorug02G0581100 Rorug03G0224600 Rorug05G0028500 Rorug05G0032700 Rorug05G0032800 Rorug05G0032900 Rorug05G0032900 Rorug05G0033000 Rorug05G0033100 Rorug05G0033300 Rorug05G0033400 Rorug05G0033900 Rorug05G0034900 Rorug05G0035000 Rorug05G0046900 Rorug05G0047500 Rorug05G0047500 Rorug05G0111600 Rorug05G0287900 Rorug05G0288700 Rorug05G0586900 Rorug06G0231700 Rorug06G0354300 Rorug06G0354400
rosa_samantha Rh1DG122200 Rh2DG686800 Rh5BG136600 Rh6AG344500 Rh6BG351900 Rh6CG359000 Rh6CG359100 Rh6CG387600 Rh6CG480500 Rh6DG344600 Rh6DG467400
rosa_wichuraiana Rw0G010250 Rw0G014820 Rw0G021400 Rw0G023100 Rw1G009450 Rw1G012100 Rw1G012180 Rw1G028360 Rw2G054260 Rw3G024370 Rw3G024420 Rw3G024470 Rw5G010850 Rw5G011010 Rw5G011020 Rw5G011050 Rw5G011080 Rw5G011120 Rw5G012300 Rw5G018470 Rw5G033830 Rw6G008930 Rw6G030070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1866
Acc36I ACCTGC 1 cut(s) 866
AccB1I GGYRCC 1 cut(s) 1412
AccI GTMKAC 4 cut(s) 456, 477, 843, 1866
AccII CGCG 3 cut(s) 86, 632, 1756
AciI CCGC 6 cut(s) 60, 84, 101, 138, 1283, 1592
AclI AACGTT 1 cut(s) 444
AclWI GGATC 3 cut(s) 632, 1594, 2318
AcoI YGGCCR 3 cut(s) 57, 1728, 2112
AcsI RAATTY 5 cut(s) 991, 1365, 1995, 2170, 2298
AcuI CTGAAG 1 cut(s) 1533
AcyI GRCGYC 2 cut(s) 1413, 1863
AfaI GTAC 4 cut(s) 259, 1199, 2195, 2272
AfiI CCNNNNNNNGG 2 cut(s) 923, 960
AgsI TTSAA 9 cut(s) 683, 722, 1204, 1573, 1615, 1971, 1994, 2100, 2303
AhlI ACTAGT 2 cut(s) 1525, 1792
AjiI CACGTC 1 cut(s) 1690
AjnI CCWGG 6 cut(s) 118, 459, 641, 898, 1000, 1070
AjuI GAANNNNNNNTTGG 2 cut(s) 407, 439
AluBI AGCT 9 cut(s) 179, 388, 666, 748, 1646, 1751, 1872, 2225, 2276
AluI AGCT 9 cut(s) 179, 388, 666, 748, 1646, 1751, 1872, 2225, 2276
Alw21I GWGCWC 2 cut(s) 854, 950
Alw26I GTCTC 4 cut(s) 860, 1502, 1680, 2180
Alw44I GTGCAC 1 cut(s) 946
AlwI GGATC 3 cut(s) 632, 1594, 2318
AlwNI CAGNNNCTG 1 cut(s) 339
Ama87I CYCGRG 3 cut(s) 194, 383, 2180
AoxI GGCC 7 cut(s) 3, 57, 166, 501, 1728, 2093, 2112
ApaLI GTGCAC 1 cut(s) 946
ApeKI GCWGC 5 cut(s) 34, 80, 388, 663, 872
ApoI RAATTY 5 cut(s) 991, 1365, 1995, 2170, 2298
Asp700I GAANNNNTTC 2 cut(s) 1199, 1830
AspA2I CCTAGG 1 cut(s) 169
AspLEI GCGC 3 cut(s) 88, 563, 1415
AspS9I GGNCC 2 cut(s) 964, 1819
AsuNHI GCTAGC 1 cut(s) 76
AvaI CYCGRG 3 cut(s) 194, 383, 2180
AvaII GGWCC 2 cut(s) 964, 1819
AvrII CCTAGG 1 cut(s) 169
BaeGI GKGCMC 2 cut(s) 950, 1642
BalI TGGCCA 1 cut(s) 2114
BanI GGYRCC 1 cut(s) 1412
BarI GAAGNNNNNNTAC 2 cut(s) 1506, 1538
BauI CACGAG 1 cut(s) 1012
BbsI GAAGAC 1 cut(s) 1050
Bbv12I GWGCWC 2 cut(s) 854, 950
BbvI GCAGC 5 cut(s) 21, 92, 375, 675, 859
BccI CCATC 7 cut(s) 212, 244, 281, 413, 581, 863, 2243
BceAI ACGGC 4 cut(s) 885, 1241, 1647, 1743
BcgI CGANNNNNNTGC 4 cut(s) 364, 398, 770, 804
BciT130I CCWGG 6 cut(s) 120, 461, 643, 900, 1002, 1072
BcoDI GTCTC 4 cut(s) 860, 1502, 1680, 2180
BcuI ACTAGT 2 cut(s) 1525, 1792
BfmI CTRYAG 2 cut(s) 873, 1867
BfoI RGCGCY 1 cut(s) 1416
BfuAI ACCTGC 1 cut(s) 866
BisI GCNGC 8 cut(s) 35, 60, 81, 84, 101, 389, 664, 873
BlnI CCTAGG 1 cut(s) 169
BlsI GCNGC 8 cut(s) 36, 61, 82, 85, 102, 390, 665, 874
BmcAI AGTACT 2 cut(s) 1199, 2195
Bme1390I CCNGG 6 cut(s) 120, 461, 643, 900, 1002, 1072
Bme18I GGWCC 2 cut(s) 964, 1819
BmeT110I CYCGRG 3 cut(s) 194, 383, 2180
BmgBI CACGTC 1 cut(s) 1690
BmgT120I GGNCC 2 cut(s) 964, 1819
BmiI GGNNCC 7 cut(s) 914, 965, 966, 1255, 1342, 1414, 1821
BmrFI CCNGG 6 cut(s) 120, 461, 643, 900, 1002, 1072
BmsI GCATC 6 cut(s) 113, 555, 1068, 1631, 1934, 2158
BmtI GCTAGC 1 cut(s) 80
BpiI GAAGAC 1 cut(s) 1050
BpmI CTGGAG 1 cut(s) 984
BpuEI CTTGAG 2 cut(s) 93, 1313
Bsa29I ATCGAT 1 cut(s) 939
BsaHI GRCGYC 2 cut(s) 1413, 1863
BsaWI WCCGGW 2 cut(s) 751, 846
BsaXI ACNNNNNCTCC 8 cut(s) 483, 513, 1242, 1272, 1640, 1670, 2177, 2207
Bsc4I CCNNNNNNNGG 2 cut(s) 923, 960
Bse1I ACTGG 1 cut(s) 889
Bse3DI GCAATG 1 cut(s) 71
BseBI CCWGG 6 cut(s) 120, 461, 643, 900, 1002, 1072
BseCI ATCGAT 1 cut(s) 939
BseGI GGATG 8 cut(s) 128, 443, 478, 573, 1059, 1155, 2092, 2149
BseLI CCNNNNNNNGG 2 cut(s) 923, 960
BseMI GCAATG 1 cut(s) 71
BseMII CTCAG 1 cut(s) 2181
BseNI ACTGG 1 cut(s) 889
BseRI GAGGAG 6 cut(s) 96, 365, 384, 1266, 1648, 2198
BseSI GKGCMC 2 cut(s) 950, 1642
BseXI GCAGC 5 cut(s) 21, 92, 375, 675, 859
BseYI CCCAGC 1 cut(s) 2200
BsgI GTGCAG 3 cut(s) 682, 810, 955
Bsh1236I CGCG 3 cut(s) 86, 632, 1756
Bsh1285I CGRYCG 1 cut(s) 847
BshFI GGCC 7 cut(s) 5, 59, 168, 503, 1730, 2095, 2114
BshNI GGYRCC 1 cut(s) 1412
BshVI ATCGAT 1 cut(s) 939
BsiEI CGRYCG 1 cut(s) 847
BsiHKAI GWGCWC 2 cut(s) 854, 950
BsiHKCI CYCGRG 3 cut(s) 194, 383, 2180
BsiSI CCGG 2 cut(s) 752, 847
BslFI GGGAC 4 cut(s) 791, 977, 1816, 2163
BslI CCNNNNNNNGG 2 cut(s) 923, 960
BsmAI GTCTC 4 cut(s) 860, 1502, 1680, 2180
BsmBI CGTCTC 2 cut(s) 860, 1680
BsmFI GGGAC 4 cut(s) 791, 977, 1816, 2163
BsmI GAATGC 1 cut(s) 2136
BsnI GGCC 7 cut(s) 5, 59, 168, 503, 1730, 2095, 2114
BsoBI CYCGRG 3 cut(s) 194, 383, 2180
Bsp1286I GDGCHC 3 cut(s) 854, 950, 1642
Bsp143I GATC 6 cut(s) 142, 214, 637, 1586, 1771, 2323
Bsp19I CCATGG 3 cut(s) 1546, 2115, 2153
BspACI CCGC 6 cut(s) 60, 84, 101, 138, 1283, 1592
BspANI GGCC 7 cut(s) 5, 59, 168, 503, 1730, 2095, 2114
BspCNI CTCAG 1 cut(s) 2182
BspDI ATCGAT 1 cut(s) 939
BspFNI CGCG 3 cut(s) 86, 632, 1756
BspLI GGNNCC 7 cut(s) 914, 965, 966, 1255, 1342, 1414, 1821
BspMAI CTGCAG 1 cut(s) 877
BspMI ACCTGC 1 cut(s) 866
BspOI GCTAGC 1 cut(s) 80
BspPI GGATC 3 cut(s) 632, 1594, 2318
BspT107I GGYRCC 1 cut(s) 1412
BsrDI GCAATG 1 cut(s) 71
BsrI ACTGG 1 cut(s) 889
BssMI GATC 6 cut(s) 142, 214, 637, 1586, 1771, 2323
BssNI GRCGYC 2 cut(s) 1413, 1863
BssSI CACGAG 1 cut(s) 1012
BssT1I CCWWGG 5 cut(s) 169, 433, 1546, 2115, 2153
Bst2BI CACGAG 1 cut(s) 1012
Bst2UI CCWGG 6 cut(s) 120, 461, 643, 900, 1002, 1072
Bst4CI ACNGT 4 cut(s) 257, 305, 1214, 1354
BstACI GRCGYC 2 cut(s) 1413, 1863
BstC8I GCNNGC 3 cut(s) 78, 896, 2294
BstDEI CTNAG 2 cut(s) 2078, 2190
BstDSI CCRYGG 4 cut(s) 1257, 1546, 2115, 2153
BstEII GGTNACC 1 cut(s) 2205
BstENI CCTNNNNNAGG 1 cut(s) 958
BstF5I GGATG 8 cut(s) 128, 443, 478, 573, 1059, 1155, 2092, 2149
BstFNI CGCG 3 cut(s) 86, 632, 1756
BstH2I RGCGCY 1 cut(s) 1416
BstHHI GCGC 3 cut(s) 88, 563, 1415
BstKTI GATC 6 cut(s) 145, 217, 640, 1589, 1774, 2326
BstMAI GTCTC 4 cut(s) 860, 1502, 1680, 2180
BstMBI GATC 6 cut(s) 142, 214, 637, 1586, 1771, 2323
BstMCI CGRYCG 1 cut(s) 847
BstMWI GCNNNNNNNGC 4 cut(s) 558, 900, 1804, 2057
BstNI CCWGG 6 cut(s) 120, 461, 643, 900, 1002, 1072
BstNSI RCATGY 1 cut(s) 2296
BstPI GGTNACC 1 cut(s) 2205
BstSCI CCNGG 6 cut(s) 118, 459, 641, 898, 1000, 1070
BstSFI CTRYAG 2 cut(s) 873, 1867
BstSLI GKGCMC 2 cut(s) 950, 1642
BstUI CGCG 3 cut(s) 86, 632, 1756
BstV1I GCAGC 5 cut(s) 21, 92, 375, 675, 859
BstV2I GAAGAC 1 cut(s) 1050
BstXI CCANNNNNNTGG 2 cut(s) 1071, 2122
Bsu15I ATCGAT 1 cut(s) 939
BsuRI GGCC 7 cut(s) 5, 59, 168, 503, 1730, 2095, 2114
BsuTUI ATCGAT 1 cut(s) 939
BtgI CCRYGG 4 cut(s) 1257, 1546, 2115, 2153
BtrI CACGTC 1 cut(s) 1690
BtsCI GGATG 8 cut(s) 128, 443, 478, 573, 1059, 1155, 2092, 2149
BtsIMutI CAGTG 1 cut(s) 896
BveI ACCTGC 1 cut(s) 866
Cac8I GCNNGC 3 cut(s) 78, 896, 2294
CaiI CAGNNNCTG 1 cut(s) 339
CfoI GCGC 3 cut(s) 88, 563, 1415
Cfr13I GGNCC 2 cut(s) 964, 1819
ClaI ATCGAT 1 cut(s) 939
CseI GACGC 3 cut(s) 1813, 1943, 2012
Csp6I GTAC 4 cut(s) 258, 1198, 2194, 2271
CviQI GTAC 4 cut(s) 258, 1198, 2194, 2271
DdeI CTNAG 2 cut(s) 2078, 2190
DinI GGCGCC 1 cut(s) 1414
DpnI GATC 6 cut(s) 144, 216, 639, 1588, 1773, 2325
DpnII GATC 6 cut(s) 142, 214, 637, 1586, 1771, 2323
EaeI YGGCCR 3 cut(s) 57, 1728, 2112
Eco130I CCWWGG 5 cut(s) 169, 433, 1546, 2115, 2153
Eco147I AGGCCT 1 cut(s) 2095
Eco32I GATATC 1 cut(s) 937
Eco47I GGWCC 2 cut(s) 964, 1819
Eco57I CTGAAG 1 cut(s) 1533
Eco88I CYCGRG 3 cut(s) 194, 383, 2180
Eco91I GGTNACC 1 cut(s) 2205
EcoNI CCTNNNNNAGG 1 cut(s) 958
EcoO109I RGGNCCY 1 cut(s) 964
EcoO65I GGTNACC 1 cut(s) 2205
EcoRI GAATTC 1 cut(s) 1365
EcoRII CCWGG 6 cut(s) 118, 459, 641, 898, 1000, 1070
EcoRV GATATC 1 cut(s) 937
EcoT14I CCWWGG 5 cut(s) 169, 433, 1546, 2115, 2153
EgeI GGCGCC 1 cut(s) 1414
EheI GGCGCC 1 cut(s) 1414
ErhI CCWWGG 5 cut(s) 169, 433, 1546, 2115, 2153
Esp3I CGTCTC 2 cut(s) 860, 1680
FalI AAGNNNNNCTT 4 cut(s) 1184, 1216, 1791, 1823
FaqI GGGAC 4 cut(s) 791, 977, 1816, 2163
FauNDI CATATG 1 cut(s) 2317
FblI GTMKAC 4 cut(s) 456, 477, 843, 1866
Fnu4HI GCNGC 8 cut(s) 35, 60, 81, 84, 101, 389, 664, 873
FokI GGATG 8 cut(s) 135, 450, 485, 560, 1046, 1162, 2099, 2136
Fsp4HI GCNGC 8 cut(s) 35, 60, 81, 84, 101, 389, 664, 873
GlaI GCGC 3 cut(s) 87, 562, 1414
GluI GCNGC 8 cut(s) 35, 60, 81, 84, 101, 389, 664, 873
GsaI CCCAGC 1 cut(s) 2204
GsuI CTGGAG 1 cut(s) 984
HaeII RGCGCY 1 cut(s) 1416
HaeIII GGCC 7 cut(s) 5, 59, 168, 503, 1730, 2095, 2114
HapII CCGG 2 cut(s) 752, 847
HgaI GACGC 3 cut(s) 1813, 1943, 2012
HhaI GCGC 3 cut(s) 88, 563, 1415
Hin1I GRCGYC 2 cut(s) 1413, 1863
Hin6I GCGC 3 cut(s) 86, 561, 1413
HinP1I GCGC 3 cut(s) 86, 561, 1413
HincII GTYRAC 2 cut(s) 301, 844
HindII GTYRAC 2 cut(s) 301, 844
HindIII AAGCTT 2 cut(s) 177, 2223
HinfI GANTC 5 cut(s) 22, 539, 782, 1327, 1990
HpaII CCGG 2 cut(s) 752, 847
Hpy188I TCNGA 5 cut(s) 491, 1037, 1627, 1741, 2221
Hpy188III TCNNGA 6 cut(s) 932, 1507, 1685, 1769, 2000, 2180
HpyAV CCTTC 8 cut(s) 271, 458, 680, 902, 1291, 1331, 2106, 2252
HpyCH4III ACNGT 4 cut(s) 257, 305, 1214, 1354
HpyCH4IV ACGT 3 cut(s) 444, 1689, 1863
HpyF10VI GCNNNNNNNGC 4 cut(s) 558, 900, 1804, 2057
HpyF3I CTNAG 2 cut(s) 2078, 2190
HpySE526I ACGT 3 cut(s) 444, 1689, 1863
Hsp92I GRCGYC 2 cut(s) 1413, 1863
HspAI GCGC 3 cut(s) 86, 561, 1413
KasI GGCGCC 1 cut(s) 1412
KflI GGGWCCC 1 cut(s) 964
Kzo9I GATC 6 cut(s) 142, 214, 637, 1586, 1771, 2323
LmnI GCTCC 5 cut(s) 372, 849, 1253, 1340, 1748
Lsp1109I GCAGC 5 cut(s) 21, 92, 375, 675, 859
LweI GCATC 6 cut(s) 113, 555, 1068, 1631, 1934, 2158
MaeII ACGT 3 cut(s) 444, 1689, 1863
MaeIII GTNAC 6 cut(s) 28, 309, 440, 878, 1859, 2205
MalI GATC 6 cut(s) 144, 216, 639, 1588, 1773, 2325
MboI GATC 6 cut(s) 142, 214, 637, 1586, 1771, 2323
MboII GAAGA 6 cut(s) 695, 811, 1055, 1298, 1627, 1959
MhlI GDGCHC 3 cut(s) 854, 950, 1642
MlsI TGGCCA 1 cut(s) 2114
MluNI TGGCCA 1 cut(s) 2114
Mly113I GGCGCC 1 cut(s) 1413
MlyI GAGTC 2 cut(s) 548, 791
MmeI TCCRAC 1 cut(s) 2103
Mox20I TGGCCA 1 cut(s) 2114
MroXI GAANNNNTTC 2 cut(s) 1199, 1830
MscI TGGCCA 1 cut(s) 2114
MseI TTAA 9 cut(s) 47, 618, 762, 1139, 1220, 1440, 1898, 2241, 2309
MslI CAYNNNNRTG 2 cut(s) 1320, 1476
Msp20I TGGCCA 1 cut(s) 2114
MspA1I CMGCKG 1 cut(s) 1283
MspI CCGG 2 cut(s) 752, 847
MspR9I CCNGG 6 cut(s) 120, 461, 643, 900, 1002, 1072
Mva1269I GAATGC 1 cut(s) 2136
MvaI CCWGG 6 cut(s) 120, 461, 643, 900, 1002, 1072
MvnI CGCG 3 cut(s) 86, 632, 1756
MwoI GCNNNNNNNGC 4 cut(s) 558, 900, 1804, 2057
NarI GGCGCC 1 cut(s) 1413
NcoI CCATGG 3 cut(s) 1546, 2115, 2153
NdeI CATATG 1 cut(s) 2317
NdeII GATC 6 cut(s) 142, 214, 637, 1586, 1771, 2323
NheI GCTAGC 1 cut(s) 76
NlaIV GGNNCC 7 cut(s) 914, 965, 966, 1255, 1342, 1414, 1821
NmuCI GTSAC 2 cut(s) 1859, 2205
NspI RCATGY 1 cut(s) 2296
PaeI GCATGC 1 cut(s) 2296
PaeR7I CTCGAG 1 cut(s) 383
PceI AGGCCT 1 cut(s) 2095
PcsI WCGNNNNNNNCGW 1 cut(s) 675
PctI GAATGC 1 cut(s) 2136
PdmI GAANNNNTTC 2 cut(s) 1199, 1830
PfeI GAWTC 3 cut(s) 22, 1327, 1990
PflFI GACNNNGTC 1 cut(s) 538
PkrI GCNGC 8 cut(s) 36, 61, 82, 85, 102, 390, 665, 874
PleI GAGTC 2 cut(s) 547, 790
PluTI GGCGCC 1 cut(s) 1416
PpsI GAGTC 2 cut(s) 547, 790
PpuMI RGGWCCY 1 cut(s) 964
Psp1406I AACGTT 1 cut(s) 444
Psp5II RGGWCCY 1 cut(s) 964
Psp6I CCWGG 6 cut(s) 118, 459, 641, 898, 1000, 1070
PspEI GGTNACC 1 cut(s) 2205
PspFI CCCAGC 1 cut(s) 2200
PspGI CCWGG 6 cut(s) 118, 459, 641, 898, 1000, 1070
PspN4I GGNNCC 7 cut(s) 914, 965, 966, 1255, 1342, 1414, 1821
PspPI GGNCC 2 cut(s) 964, 1819
PspPPI RGGWCCY 1 cut(s) 964
PspXI VCTCGAGB 1 cut(s) 383
PstI CTGCAG 1 cut(s) 877
PstNI CAGNNNCTG 1 cut(s) 339
PsyI GACNNNGTC 1 cut(s) 538
RsaI GTAC 4 cut(s) 259, 1199, 2195, 2272
RsaNI GTAC 4 cut(s) 258, 1198, 2194, 2271
RseI CAYNNNNRTG 2 cut(s) 1320, 1476
SalI GTCGAC 1 cut(s) 842
SaqAI TTAA 9 cut(s) 47, 618, 762, 1139, 1220, 1440, 1898, 2241, 2309
SatI GCNGC 8 cut(s) 35, 60, 81, 84, 101, 389, 664, 873
Sau3AI GATC 6 cut(s) 142, 214, 637, 1586, 1771, 2323
Sau96I GGNCC 2 cut(s) 964, 1819
ScaI AGTACT 2 cut(s) 1199, 2195
SchI GAGTC 2 cut(s) 548, 791
ScrFI CCNGG 6 cut(s) 120, 461, 643, 900, 1002, 1072
SduI GDGCHC 3 cut(s) 854, 950, 1642
SfaNI GCATC 6 cut(s) 113, 555, 1068, 1631, 1934, 2158
SfcI CTRYAG 2 cut(s) 873, 1867
SfoI GGCGCC 1 cut(s) 1414
Sfr274I CTCGAG 1 cut(s) 383
SinI GGWCC 2 cut(s) 964, 1819
SlaI CTCGAG 1 cut(s) 383
SmiMI CAYNNNNRTG 2 cut(s) 1320, 1476
SmlI CTYRAG 3 cut(s) 108, 383, 1292
SmoI CTYRAG 3 cut(s) 108, 383, 1292
SpeI ACTAGT 2 cut(s) 1525, 1792
SphI GCATGC 1 cut(s) 2296
SseBI AGGCCT 1 cut(s) 2095
SsiI CCGC 6 cut(s) 60, 84, 101, 138, 1283, 1592
SspDI GGCGCC 1 cut(s) 1412
StuI AGGCCT 1 cut(s) 2095
StyD4I CCNGG 6 cut(s) 118, 459, 641, 898, 1000, 1070
StyI CCWWGG 5 cut(s) 169, 433, 1546, 2115, 2153
TaaI ACNGT 4 cut(s) 257, 305, 1214, 1354
TaiI ACGT 3 cut(s) 447, 1692, 1866
TaqI TCGA 7 cut(s) 384, 599, 843, 931, 939, 1506, 1684
TaqII GACCGA 1 cut(s) 551
TatI WGTACW 2 cut(s) 1197, 2193
TauI GCSGC 3 cut(s) 62, 86, 103
TfiI GAWTC 3 cut(s) 22, 1327, 1990
Tru1I TTAA 9 cut(s) 47, 618, 762, 1139, 1220, 1440, 1898, 2241, 2309
Tru9I TTAA 9 cut(s) 47, 618, 762, 1139, 1220, 1440, 1898, 2241, 2309
TscAI CASTG 1 cut(s) 896
TseFI GTSAC 2 cut(s) 1859, 2205
TseI GCWGC 5 cut(s) 34, 80, 388, 663, 872
Tsp45I GTSAC 2 cut(s) 1859, 2205
TspDTI ATGAA 9 cut(s) 664, 1178, 1587, 1750, 1823, 2103, 2117, 2163, 2271
TspRI CASTG 1 cut(s) 896
Tth111I GACNNNGTC 1 cut(s) 538
VneI GTGCAC 1 cut(s) 946
VpaK11BI GGWCC 2 cut(s) 964, 1819
XagI CCTNNNNNAGG 1 cut(s) 958
XapI RAATTY 5 cut(s) 991, 1365, 1995, 2170, 2298
XbaI TCTAGA 1 cut(s) 1768
XceI RCATGY 1 cut(s) 2296
XhoI CTCGAG 1 cut(s) 383
XmaJI CCTAGG 1 cut(s) 169
XmiI GTMKAC 4 cut(s) 456, 477, 843, 1866
XmnI GAANNNNTTC 2 cut(s) 1199, 1830
ZraI GACGTC 1 cut(s) 1864
ZrmI AGTACT 2 cut(s) 1199, 2195
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.