RchiOBHm_Chr5g0016931

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000066
Physical Location & Seq
Reverse (-)
11654434 .. 11657408
2975 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29727

Sequence Viewer

Length: 2331 bp
ATGGCCTCATTACATGACAGGATTCTTGTTACGCTGCTCTCTTTGTTAATGTTAGTGGCCGCAACAACAACATTGCTAGCAGCGCAGACCCTAGTGCCCCCTCCTCAAGCCAAGCCAGGATGCCGAGAAAAGTGCGGTGATCTCACAATCCCATACCCGTTTGGCCTAGGCGAAGCTTGTTCCATGCGACCCGAGTTCACCATCACTTGCGATCAATCGACCACAGCAACCCCATCAGCAAACTTTACAAACAGTACCTTCACCTTTCCCATCACCATTACTAACTTTTCCCTTGTTGACGGTGAGTTACAAGTAATGCAGGACATAGCCAGAAGCTGCTATGACGAGGAGGGCAATCATACAAAAAGGCGCAGTTCACTCAAGCTGCCTCCTTCTTACACCATCTCAACCAAAAACAGCTTCTTTACCCTTGGATGTAACGTTTTAGCAGTCTATCAGCAAGGTGTGGATGTGGACTACCAAATCGGAGTAAATGTGGCCTTATGTATGGACATTCTTGGCAAGGAATTGACCGAGGCTTGCATCGGCGTTGGGTGCGCCCAAACTTCCATCCCTAGCGGACTGCAAAACATTTCGATTGGAGTGGCTTCACTTAACGGGTATAACGCGAGTGATCCCTGGGAATCAAAGTACAAGTGCAGCTACGCTTTCATCGTTGAAGAAGGCAACTTCACATTCGCACCGAAAACAAGTTTTGAACAACTGAATACAACAAATCAGCAGCTTCCGGTGGCTGTTAATTGGGCAATCGGGGACGAGTCATGTGCAGTAGCGAAGAACCTAACTGATTATTCGTGCAAAGAGAATACTATTTGTGTCGACCGGAGCACCATCACTAATGAGACGGCTGCAGGTTACATTTGCCAGTGCTTGCCAGGCTACGAAGGGAACCCATACCTCGGTTGTCGAGATATCGATGAGTGCACGACCTCATCAAGGGACCCCTGCCCGAATGGAAAGTGCGTAAATTCACCTCCAGGGAATTACTCGTGTAAATGTAATAAAGGGTTCAGAAACCAAGACGACATGACTTGCATCCCATATTCCTGGTCAAACAACACTTCCCTAAAAATATCATTAGGTGTCAGTATAGGCTTCTTGGTTTTAGTGGTCTTAATTTCGTGGATGTATTGGGAAATGAACAAAAGGAGGCTCATCAAACTCAAAAAGAAGTACTTCAAAGAAAACGGTGGCTTAACATTACAACAACAACTCGCTAGTCATGGAGGAGCCGTGGAAACAACAAAAATCTTTACAGCGGAAGAACTTGAGAAGGCAACAAACAATTACCATACAGATGAGATTCTTGGTGAAGGAGCCTATGGAACAGTTTACAAAGGAATTCTACCTAATAAGAAAGCTGTTGCCATAAAAAAATCAAAAATTGGCGCCCCAACTCAAAGTGAACAGTTCGTTAATGAGGTTATCGTTCTCTCTCAAATCAACCACAGGAATGTAGTAAAACTATTGGGTTGTTGTTTTGAGACTGAAGTACCTTTACTAGTTTATGAGTACATTACACATGGCACTCTTTTTGAGCATATATTCAATAAGGGAAAAGGATCACCACTTTCATGGGAATTACGATTGAAGATAGCATCGGAAACGGCAGGGGCACTAGCTTACTTACACTCCTCTACTTCCACACCAATCATACATCGAGACGTGAAAGCAACAAATATACTATTAGATGACAATTACACGGCCAAAGTGTCCGATTTTGGAGCTTCACGTTTCATTCCTCTAGATCAAACTGAACTAGCAACACTAGTGCAAGGGACGCTTGGCTACTTGGACCCTGAATACTTTCATTCCAATCAACTGACTGAAAAGAGTGACGTTTACAGCTTTGGAGTTGTCCTAGCAGAGTTATTAACGAGCAGAGTGGCACTTTCTTTCGCTAGACCTGACGCAGAGAGATGCCTAGCAAGTTTCTTTGTTTCTTCAATAGAGAAAGATTGCTTGATTGAAATTCTTGACGCTGACATAGTGAATGAGGGGAACATAGACACAGCAGAGCAAGTTGCTTATCTCGCAAAAAGATGTCTAGGGATAAGAGGGGAGGAAAGGCCTTCAATGAAAGAAGTGGCCATGGAGTTGGAGGGAATGCAAAATACAGCGAAGCATCCATGGCGAAATAATGCAAATTTATGTCCCGAGGAGACTGAGTACTTGCTGGGGTCACCTATCAATCCAGAAGCTTACATTGTGAATGTTAAAGGTGATGGTGATGACGGATCTAGTGGTACAACCAGTGGGTATGACAGCATGCAAATTCAAATGTTAATGCCATACGATGATGCGCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK hEGF

Protein Analysis

776

Amino Acids

85.21

Weight (kDa)

5.07

Isoelectric Point (pI)

36.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 41 - 103 1.8e-09 Wall-associated receptor kinase galacturonan-binding
Pkinase PF00069 437 - 703 2.8e-45 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 439 - 707 8.4e-47 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21210 AT1G21230 AT1G21240 AT1G21240 AT1G21245 AT1G21250 AT1G21270 AT1G22720 AT3G25490
fragaria_vesca FvH4_2g35051 FvH4_2g35051 FvH4_2g35060 FvH4_3g10281 FvH4_3g10285 FvH4_3g10286 FvH4_3g10311 FvH4_3g10311 FvH4_3g10320 FvH4_3g10332 FvH4_3g10340 FvH4_3g10350 FvH4_3g10360 FvH4_3g10390 FvH4_3g10412 FvH4_3g10413 FvH4_3g10461 FvH4_3g10462 FvH4_3g10470 FvH4_3g10510 FvH4_3g10520 FvH4_3g10530 FvH4_3g10550 FvH4_3g10560 FvH4_3g10611 FvH4_3g11730 FvH4_6g53140 FvH4_6g53142 FvH4_7g31880 FvH4_7g31910 FvH4_7g31920
malus_domestica MD05G1269900.v1.1 MD05G1270000.v1.1 MD05G1270200.v1.1 MD05G1270400.v1.1 MD05G1270500.v1.1 MD05G1270600.v1.1 MD05G1270800.v1.1 MD05G1270900.v1.1 MD07G1183800.v1.1 MD08G1107600.v1.1 MD10G1248500.v1.1 MD10G1249200.v1.1 MD10G1249500.v1.1 MD10G1250000.v1.1 MD10G1250500.v1.1 MD10G1250900.v1.1 MD10G1251200.v1.1 MD10G1251400.v1.1 MD13G1004600.v1.1 MD15G1088400.v1.1
prunus_persica Prupe.1G442400_v2.0.a1 Prupe.1G442500_v2.0.a1 Prupe.2G223400_v2.0.a1 Prupe.4G091900_v2.0.a1 Prupe.4G093200_v2.0.a1 Prupe.4G093300_v2.0.a1 Prupe.4G093400_v2.0.a1 Prupe.4G093500_v2.0.a1 Prupe.4G094100_v2.0.a1 Prupe.4G094200_v2.0.a1 Prupe.4G094300_v2.0.a1 Prupe.6G358300_v2.0.a1
pyrus_communis pycom08g08990 pycom10g20660 pycom10g20670 pycom10g20680 pycom10g20710 pycom10g20740 pycom13g00400 pycom15g08290
rosa_chinensis RchiOBHm_Chr1g0326861 RchiOBHm_Chr1g0326871 RchiOBHm_Chr1g0331391 RchiOBHm_Chr1g0336911 RchiOBHm_Chr1g0364001 RchiOBHm_Chr2g0174541 RchiOBHm_Chr2g0175041 RchiOBHm_Chr2g0175101 RchiOBHm_Chr2g0175121 RchiOBHm_Chr3g0486331 RchiOBHm_Chr5g0015581 RchiOBHm_Chr5g0016551 RchiOBHm_Chr5g0016561 RchiOBHm_Chr5g0016581 RchiOBHm_Chr5g0016601 RchiOBHm_Chr5g0016611 RchiOBHm_Chr5g0016641 RchiOBHm_Chr5g0016661 RchiOBHm_Chr5g0016691 RchiOBHm_Chr5g0016701 RchiOBHm_Chr5g0016731 RchiOBHm_Chr5g0016741 RchiOBHm_Chr5g0016811 RchiOBHm_Chr5g0016841 RchiOBHm_Chr5g0016871 RchiOBHm_Chr5g0016881 RchiOBHm_Chr5g0016931 RchiOBHm_Chr5g0017021 RchiOBHm_Chr5g0017041 RchiOBHm_Chr5g0017071 RchiOBHm_Chr5g0017091 RchiOBHm_Chr5g0017121 RchiOBHm_Chr5g0017141 RchiOBHm_Chr5g0017291 RchiOBHm_Chr5g0017321 RchiOBHm_Chr5g0017401 RchiOBHm_Chr5g0019001 RchiOBHm_Chr5g0019071 RchiOBHm_Chr5g0028941 RchiOBHm_Chr5g0052141 RchiOBHm_Chr5g0054721 RchiOBHm_Chr5g0054801 RchiOBHm_Chr5g0054931 RchiOBHm_Chr5g0054941 RchiOBHm_Chr6g0259291 RchiOBHm_Chr6g0292991 RchiOBHm_Chr6g0306911
rosa_laevigata RLG00000009465 RLG00000009468 RLG00000010755 RLG00000011976 RLG00000014541 RLG00000014542 RLG00000015169 RLG00000022308 RLG00000027529 RLG00000029324 RLG00000030244 RLG00000030245 RLG00000032210 RLG00000032211 RLG00000032257 RLG00000032259 RLG00000032260 RLG00000032261 RLG00000032262 RLG00000032268 RLG00000032269 RLG00000032272 RLG00000032282 RLG00000032283 RLG00000032308 RLG00000032413 RLG00000033129 RLG00000034945
rosa_multiflora Rmu_co8241929.1_g000001 Rmu_co8269227.1_g000001 Rmu_co8461259.1_g000001 Rmu_co8495385.1_g000001 Rmu_sc0000028.1_g000003 Rmu_sc0000083.1_g000009 Rmu_sc0000083.1_g000010 Rmu_sc0000083.1_g000031 Rmu_sc0000083.1_g000043 Rmu_sc0000083.1_g000046 Rmu_sc0000083.1_g000047 Rmu_sc0000563.1_g000024 Rmu_sc0000842.1_g000012 Rmu_sc0001235.1_g000001 Rmu_sc0001235.1_g000005 Rmu_sc0001235.1_g000009 Rmu_sc0001235.1_g000015 Rmu_sc0001235.1_g000016 Rmu_sc0001235.1_g000017 Rmu_sc0001473.1_g000036 Rmu_sc0001607.1_g000016 Rmu_sc0001788.1_g000006 Rmu_sc0002018.1_g000008 Rmu_sc0002028.1_g000001 Rmu_sc0002680.1_g000011 Rmu_sc0002798.1_g000023 Rmu_sc0002886.1_g000023 Rmu_sc0002989.1_g000012 Rmu_sc0003064.1_g000009 Rmu_sc0003382.1_g000006 Rmu_sc0003630.1_g000002 Rmu_sc0003630.1_g000047 Rmu_sc0003630.1_g000066 Rmu_sc0003630.1_g000067 Rmu_sc0003649.1_g000010 Rmu_sc0003688.1_g000005 Rmu_sc0003920.1_g000007 Rmu_sc0004765.1_g000007 Rmu_sc0004765.1_g000008 Rmu_sc0004920.1_g000011 Rmu_sc0005223.1_g000008 Rmu_sc0005223.1_g000010 Rmu_sc0005223.1_g000015 Rmu_sc0005223.1_g000016 Rmu_sc0005319.1_g000014 Rmu_sc0005319.1_g000015 Rmu_sc0005319.1_g000018 Rmu_sc0005319.1_g000019 Rmu_sc0005319.1_g000025 Rmu_sc0005418.1_g000007 Rmu_sc0005418.1_g000012 Rmu_sc0006106.1_g000011 Rmu_sc0006914.1_g000012 Rmu_sc0007023.1_g000001 Rmu_sc0007662.1_g000001 Rmu_sc0007920.1_g000002 Rmu_sc0007920.1_g000013 Rmu_sc0008053.1_g000016 Rmu_sc0008053.1_g000017 Rmu_sc0008053.1_g000018 Rmu_sc0008053.1_g000023 Rmu_sc0008053.1_g000050 Rmu_sc0009042.1_g000007 Rmu_sc0009042.1_g000008 Rmu_sc0010642.1_g000008 Rmu_sc0011109.1_g000002 Rmu_sc0011314.1_g000006 Rmu_sc0011974.1_g000006 Rmu_sc0012179.1_g000009 Rmu_sc0015169.1_g000001 Rmu_sc0016368.1_g000002 Rmu_sc0022659.1_g000001 Rmu_sc0027371.1_g000001 Rmu_sc0028213.1_g000001 Rmu_sc0034526.1_g000001 Rmu_sc0039366.1_g000002 Rmu_ssc0000050.1_g000084 Rmu_ssc0000050.1_g000088 Rmu_ssc0000167.1_g000017
rosa_roxburghii Rroxscaffold_1G00011340 Rroxscaffold_1G00025710 Rroxscaffold_1G00025870 Rroxscaffold_1G00026070 Rroxscaffold_1G00026170 Rroxscaffold_1G00026200 Rroxscaffold_1G00026270 Rroxscaffold_1G00050950 Rroxscaffold_1G00058900 Rroxscaffold_1G00060040 Rroxscaffold_1G00060060 Rroxscaffold_1G00060070 Rroxscaffold_1G00060080 Rroxscaffold_1G00060110 Rroxscaffold_1G00060120 Rroxscaffold_1G00060140 Rroxscaffold_1G00060170 Rroxscaffold_1G00060210 Rroxscaffold_1G00060220 Rroxscaffold_1G00060240 Rroxscaffold_1G00060250 Rroxscaffold_1G00060260 Rroxscaffold_1G00060300 Rroxscaffold_1G00060320 Rroxscaffold_1G00060330 Rroxscaffold_1G00060900 Rroxscaffold_1G00060920 Rroxscaffold_1G00073980 Rroxscaffold_2G00077560 Rroxscaffold_2G00077580 Rroxscaffold_2G00146990 Rroxscaffold_3G00236200 Rroxscaffold_3G00236210 Rroxscaffold_3G00236220 Rroxscaffold_4G00292760 Rroxscaffold_4G00315200 Rroxscaffold_4G00315240 Rroxscaffold_4G00326240 Rroxscaffold_4G00328980 Rroxscaffold_5G00341890 Rroxscaffold_7G00161350 Rroxscaffold_7G00174490 Rroxscaffold_7G00206550
rosa_rugosa Rorug01G0093000 Rorug01G0093100 Rorug01G0128800 Rorug01G0312000 Rorug01G0312000 Rorug02G0581100 Rorug03G0224600 Rorug05G0028500 Rorug05G0032700 Rorug05G0032800 Rorug05G0032900 Rorug05G0032900 Rorug05G0033000 Rorug05G0033100 Rorug05G0033300 Rorug05G0033400 Rorug05G0033900 Rorug05G0034900 Rorug05G0035000 Rorug05G0046900 Rorug05G0047500 Rorug05G0047500 Rorug05G0111600 Rorug05G0287900 Rorug05G0288700 Rorug05G0586900 Rorug06G0231700 Rorug06G0354300 Rorug06G0354400
rosa_samantha Rh1DG122200 Rh2DG686800 Rh5BG136600 Rh6AG344500 Rh6BG351900 Rh6CG359000 Rh6CG359100 Rh6CG387600 Rh6CG480500 Rh6DG344600 Rh6DG467400
rosa_wichuraiana Rw0G010250 Rw0G014820 Rw0G021400 Rw0G023100 Rw1G009450 Rw1G012100 Rw1G012180 Rw1G028360 Rw2G054260 Rw3G024370 Rw3G024420 Rw3G024470 Rw5G010850 Rw5G011010 Rw5G011020 Rw5G011050 Rw5G011080 Rw5G011120 Rw5G012300 Rw5G018470 Rw5G033830 Rw6G008930 Rw6G030070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 863
AccB1I GGYRCC 1 cut(s) 1409
AccI GTMKAC 1 cut(s) 840
AccII CGCG 2 cut(s) 629, 2326
AciI CCGC 4 cut(s) 60, 135, 579, 1280
AclI AACGTT 1 cut(s) 441
AclWI GGATC 3 cut(s) 629, 1591, 2266
AcoI YGGCCR 3 cut(s) 57, 1725, 2109
AcsI RAATTY 5 cut(s) 988, 1362, 1992, 2167, 2295
AcuI CTGAAG 1 cut(s) 1530
AcyI GRCGYC 1 cut(s) 1410
AfaI GTAC 7 cut(s) 256, 653, 1196, 1515, 1535, 2192, 2269
AfiI CCNNNNNNNGG 2 cut(s) 920, 957
AgsI TTSAA 9 cut(s) 680, 719, 1201, 1570, 1612, 1968, 1991, 2097, 2300
AhlI ACTAGT 2 cut(s) 1522, 1789
AjiI CACGTC 1 cut(s) 1687
AjnI CCWGG 5 cut(s) 115, 638, 895, 997, 1067
AjuI GAANNNNNNNTTGG 2 cut(s) 404, 436
Alw21I GWGCWC 2 cut(s) 851, 947
Alw26I GTCTC 4 cut(s) 857, 1499, 1677, 2177
Alw44I GTGCAC 1 cut(s) 943
AlwI GGATC 3 cut(s) 629, 1591, 2266
AlwNI CAGNNNCTG 1 cut(s) 336
Ama87I CYCGRG 2 cut(s) 191, 2177
AoxI GGCC 7 cut(s) 3, 57, 163, 498, 1725, 2090, 2109
ApaLI GTGCAC 1 cut(s) 943
ApeKI GCWGC 7 cut(s) 34, 80, 336, 385, 660, 742, 869
ApoI RAATTY 5 cut(s) 988, 1362, 1992, 2167, 2295
Asp700I GAANNNNTTC 2 cut(s) 1196, 1827
AspA2I CCTAGG 1 cut(s) 166
AspLEI GCGC 5 cut(s) 85, 372, 560, 1412, 2326
AspS9I GGNCC 2 cut(s) 961, 1816
AsuNHI GCTAGC 1 cut(s) 76
AvaI CYCGRG 2 cut(s) 191, 2177
AvaII GGWCC 2 cut(s) 961, 1816
AvrII CCTAGG 1 cut(s) 166
BaeGI GKGCMC 3 cut(s) 99, 947, 1639
BaeI ACNNNNGTAYC 2 cut(s) 1497, 1530
BalI TGGCCA 1 cut(s) 2111
BanI GGYRCC 1 cut(s) 1409
BarI GAAGNNNNNNTAC 2 cut(s) 1503, 1535
BauI CACGAG 1 cut(s) 1009
Bbv12I GWGCWC 2 cut(s) 851, 947
BbvI GCAGC 7 cut(s) 21, 92, 323, 372, 672, 754, 856
BccI CCATC 7 cut(s) 209, 241, 278, 410, 578, 860, 2240
BceAI ACGGC 4 cut(s) 882, 1238, 1644, 1740
BcgI CGANNNNNNTGC 4 cut(s) 114, 148, 767, 801
BciT130I CCWGG 5 cut(s) 117, 640, 897, 999, 1069
BcoDI GTCTC 4 cut(s) 857, 1499, 1677, 2177
BcuI ACTAGT 2 cut(s) 1522, 1789
BfmI CTRYAG 1 cut(s) 870
BfoI RGCGCY 1 cut(s) 1413
BfuAI ACCTGC 1 cut(s) 863
BisI GCNGC 8 cut(s) 35, 60, 81, 337, 386, 661, 743, 870
BlnI CCTAGG 1 cut(s) 166
BlsI GCNGC 8 cut(s) 36, 61, 82, 338, 387, 662, 744, 871
BmcAI AGTACT 2 cut(s) 1196, 2192
Bme1390I CCNGG 5 cut(s) 117, 640, 897, 999, 1069
Bme18I GGWCC 2 cut(s) 961, 1816
BmeT110I CYCGRG 2 cut(s) 191, 2177
BmgBI CACGTC 1 cut(s) 1687
BmgT120I GGNCC 2 cut(s) 961, 1816
BmiI GGNNCC 7 cut(s) 911, 962, 963, 1252, 1339, 1411, 1818
BmrFI CCNGG 5 cut(s) 117, 640, 897, 999, 1069
BmsI GCATC 7 cut(s) 110, 552, 1065, 1628, 1931, 2155, 2311
BmtI GCTAGC 1 cut(s) 80
BpmI CTGGAG 1 cut(s) 981
BpuEI CTTGAG 3 cut(s) 90, 365, 1310
Bsa29I ATCGAT 1 cut(s) 936
BsaHI GRCGYC 1 cut(s) 1410
BsaWI WCCGGW 2 cut(s) 748, 843
BsaXI ACNNNNNCTCC 8 cut(s) 480, 510, 1239, 1269, 1637, 1667, 2174, 2204
Bsc4I CCNNNNNNNGG 2 cut(s) 920, 957
Bse1I ACTGG 2 cut(s) 886, 2274
Bse3DI GCAATG 1 cut(s) 71
BseBI CCWGG 5 cut(s) 117, 640, 897, 999, 1069
BseCI ATCGAT 1 cut(s) 936
BseGI GGATG 7 cut(s) 125, 440, 475, 570, 1056, 1152, 2146
BseLI CCNNNNNNNGG 2 cut(s) 920, 957
BseMI GCAATG 1 cut(s) 71
BseMII CTCAG 1 cut(s) 2178
BseNI ACTGG 2 cut(s) 886, 2274
BseRI GAGGAG 5 cut(s) 93, 362, 1263, 1645, 2195
BseSI GKGCMC 3 cut(s) 99, 947, 1639
BseXI GCAGC 7 cut(s) 21, 92, 323, 372, 672, 754, 856
BseYI CCCAGC 1 cut(s) 2197
BsgI GTGCAG 2 cut(s) 679, 807
Bsh1236I CGCG 2 cut(s) 629, 2326
Bsh1285I CGRYCG 1 cut(s) 844
BshFI GGCC 7 cut(s) 5, 59, 165, 500, 1727, 2092, 2111
BshNI GGYRCC 1 cut(s) 1409
BshVI ATCGAT 1 cut(s) 936
BsiEI CGRYCG 1 cut(s) 844
BsiHKAI GWGCWC 2 cut(s) 851, 947
BsiHKCI CYCGRG 2 cut(s) 191, 2177
BsiSI CCGG 2 cut(s) 749, 844
BslFI GGGAC 4 cut(s) 788, 974, 1813, 2160
BslI CCNNNNNNNGG 2 cut(s) 920, 957
BsmAI GTCTC 4 cut(s) 857, 1499, 1677, 2177
BsmBI CGTCTC 2 cut(s) 857, 1677
BsmFI GGGAC 4 cut(s) 788, 974, 1813, 2160
BsmI GAATGC 1 cut(s) 2133
BsnI GGCC 7 cut(s) 5, 59, 165, 500, 1727, 2092, 2111
BsoBI CYCGRG 2 cut(s) 191, 2177
Bsp1286I GDGCHC 4 cut(s) 99, 851, 947, 1639
Bsp143I GATC 6 cut(s) 139, 211, 634, 1583, 1768, 2258
Bsp19I CCATGG 2 cut(s) 2112, 2150
BspACI CCGC 4 cut(s) 60, 135, 579, 1280
BspANI GGCC 7 cut(s) 5, 59, 165, 500, 1727, 2092, 2111
BspCNI CTCAG 1 cut(s) 2179
BspDI ATCGAT 1 cut(s) 936
BspFNI CGCG 2 cut(s) 629, 2326
BspLI GGNNCC 7 cut(s) 911, 962, 963, 1252, 1339, 1411, 1818
BspMAI CTGCAG 1 cut(s) 874
BspMI ACCTGC 1 cut(s) 863
BspOI GCTAGC 1 cut(s) 80
BspPI GGATC 3 cut(s) 629, 1591, 2266
BspT107I GGYRCC 1 cut(s) 1409
BsrDI GCAATG 1 cut(s) 71
BsrI ACTGG 2 cut(s) 886, 2274
BssMI GATC 6 cut(s) 139, 211, 634, 1583, 1768, 2258
BssNI GRCGYC 1 cut(s) 1410
BssSI CACGAG 1 cut(s) 1009
BssT1I CCWWGG 4 cut(s) 166, 430, 2112, 2150
Bst2BI CACGAG 1 cut(s) 1009
Bst2UI CCWGG 5 cut(s) 117, 640, 897, 999, 1069
Bst4CI ACNGT 5 cut(s) 254, 302, 1211, 1351, 1431
BstACI GRCGYC 1 cut(s) 1410
BstC8I GCNNGC 4 cut(s) 78, 541, 893, 2291
BstDEI CTNAG 1 cut(s) 2187
BstDSI CCRYGG 3 cut(s) 1254, 2112, 2150
BstEII GGTNACC 1 cut(s) 2202
BstENI CCTNNNNNAGG 1 cut(s) 955
BstF5I GGATG 7 cut(s) 125, 440, 475, 570, 1056, 1152, 2146
BstFNI CGCG 2 cut(s) 629, 2326
BstH2I RGCGCY 1 cut(s) 1413
BstHHI GCGC 5 cut(s) 85, 372, 560, 1412, 2326
BstKTI GATC 6 cut(s) 142, 214, 637, 1586, 1771, 2261
BstMAI GTCTC 4 cut(s) 857, 1499, 1677, 2177
BstMBI GATC 6 cut(s) 139, 211, 634, 1583, 1768, 2258
BstMCI CGRYCG 1 cut(s) 844
BstMWI GCNNNNNNNGC 6 cut(s) 82, 555, 897, 1801, 2054, 2152
BstNI CCWGG 5 cut(s) 117, 640, 897, 999, 1069
BstNSI RCATGY 1 cut(s) 2293
BstPI GGTNACC 1 cut(s) 2202
BstSCI CCNGG 5 cut(s) 115, 638, 895, 997, 1067
BstSFI CTRYAG 1 cut(s) 870
BstSLI GKGCMC 3 cut(s) 99, 947, 1639
BstUI CGCG 2 cut(s) 629, 2326
BstV1I GCAGC 7 cut(s) 21, 92, 323, 372, 672, 754, 856
BstX2I RGATCY 1 cut(s) 2258
BstXI CCANNNNNNTGG 3 cut(s) 1068, 1596, 2119
BstYI RGATCY 1 cut(s) 2258
Bsu15I ATCGAT 1 cut(s) 936
BsuRI GGCC 7 cut(s) 5, 59, 165, 500, 1727, 2092, 2111
BsuTUI ATCGAT 1 cut(s) 936
BtgI CCRYGG 3 cut(s) 1254, 2112, 2150
BtrI CACGTC 1 cut(s) 1687
BtsCI GGATG 7 cut(s) 125, 440, 475, 570, 1056, 1152, 2146
BtsIMutI CAGTG 2 cut(s) 893, 2281
BveI ACCTGC 1 cut(s) 863
Cac8I GCNNGC 4 cut(s) 78, 541, 893, 2291
CaiI CAGNNNCTG 1 cut(s) 336
CfoI GCGC 5 cut(s) 85, 372, 560, 1412, 2326
Cfr13I GGNCC 2 cut(s) 961, 1816
ClaI ATCGAT 1 cut(s) 936
CseI GACGC 3 cut(s) 1810, 1940, 2009
Csp6I GTAC 7 cut(s) 255, 652, 1195, 1514, 1534, 2191, 2268
CviQI GTAC 7 cut(s) 255, 652, 1195, 1514, 1534, 2191, 2268
DdeI CTNAG 1 cut(s) 2187
DinI GGCGCC 1 cut(s) 1411
DpnI GATC 6 cut(s) 141, 213, 636, 1585, 1770, 2260
DpnII GATC 6 cut(s) 139, 211, 634, 1583, 1768, 2258
EaeI YGGCCR 3 cut(s) 57, 1725, 2109
Eco130I CCWWGG 4 cut(s) 166, 430, 2112, 2150
Eco147I AGGCCT 1 cut(s) 2092
Eco32I GATATC 1 cut(s) 934
Eco47I GGWCC 2 cut(s) 961, 1816
Eco57I CTGAAG 1 cut(s) 1530
Eco88I CYCGRG 2 cut(s) 191, 2177
Eco91I GGTNACC 1 cut(s) 2202
EcoNI CCTNNNNNAGG 1 cut(s) 955
EcoO109I RGGNCCY 1 cut(s) 961
EcoO65I GGTNACC 1 cut(s) 2202
EcoRI GAATTC 1 cut(s) 1362
EcoRII CCWGG 5 cut(s) 115, 638, 895, 997, 1067
EcoRV GATATC 1 cut(s) 934
EcoT14I CCWWGG 4 cut(s) 166, 430, 2112, 2150
EgeI GGCGCC 1 cut(s) 1411
EheI GGCGCC 1 cut(s) 1411
ErhI CCWWGG 4 cut(s) 166, 430, 2112, 2150
Esp3I CGTCTC 2 cut(s) 857, 1677
FalI AAGNNNNNCTT 4 cut(s) 1181, 1213, 1788, 1820
FaqI GGGAC 4 cut(s) 788, 974, 1813, 2160
FblI GTMKAC 1 cut(s) 840
Fnu4HI GCNGC 8 cut(s) 35, 60, 81, 337, 386, 661, 743, 870
FokI GGATG 7 cut(s) 132, 447, 482, 557, 1043, 1159, 2133
Fsp4HI GCNGC 8 cut(s) 35, 60, 81, 337, 386, 661, 743, 870
GlaI GCGC 5 cut(s) 84, 371, 559, 1411, 2325
GluI GCNGC 8 cut(s) 35, 60, 81, 337, 386, 661, 743, 870
GsaI CCCAGC 1 cut(s) 2201
GsuI CTGGAG 1 cut(s) 981
HaeII RGCGCY 1 cut(s) 1413
HaeIII GGCC 7 cut(s) 5, 59, 165, 500, 1727, 2092, 2111
HapII CCGG 2 cut(s) 749, 844
HgaI GACGC 3 cut(s) 1810, 1940, 2009
HhaI GCGC 5 cut(s) 85, 372, 560, 1412, 2326
Hin1I GRCGYC 1 cut(s) 1410
Hin6I GCGC 5 cut(s) 83, 370, 558, 1410, 2324
HinP1I GCGC 5 cut(s) 83, 370, 558, 1410, 2324
HincII GTYRAC 2 cut(s) 298, 841
HindII GTYRAC 2 cut(s) 298, 841
HindIII AAGCTT 2 cut(s) 174, 2220
HinfI GANTC 4 cut(s) 22, 644, 779, 1324
HpaII CCGG 2 cut(s) 749, 844
Hpy166II GTNNAC 9 cut(s) 198, 298, 377, 475, 841, 945, 1354, 1427, 1864
Hpy188I TCNGA 4 cut(s) 488, 1034, 1624, 1738
Hpy188III TCNNGA 6 cut(s) 929, 1682, 1766, 1997, 2177, 2216
Hpy8I GTNNAC 9 cut(s) 198, 298, 377, 475, 841, 945, 1354, 1427, 1864
HpyAV CCTTC 7 cut(s) 268, 402, 677, 899, 1288, 1328, 2103
HpyCH4III ACNGT 5 cut(s) 254, 302, 1211, 1351, 1431
HpyCH4IV ACGT 4 cut(s) 441, 1686, 1753, 1860
HpyF10VI GCNNNNNNNGC 6 cut(s) 82, 555, 897, 1801, 2054, 2152
HpyF3I CTNAG 1 cut(s) 2187
HpySE526I ACGT 4 cut(s) 441, 1686, 1753, 1860
Hsp92I GRCGYC 1 cut(s) 1410
HspAI GCGC 5 cut(s) 83, 370, 558, 1410, 2324
KasI GGCGCC 1 cut(s) 1409
KflI GGGWCCC 1 cut(s) 961
Kzo9I GATC 6 cut(s) 139, 211, 634, 1583, 1768, 2258
LmnI GCTCC 4 cut(s) 846, 1250, 1337, 1745
Lsp1109I GCAGC 7 cut(s) 21, 92, 323, 372, 672, 754, 856
LweI GCATC 7 cut(s) 110, 552, 1065, 1628, 1931, 2155, 2311
MaeII ACGT 4 cut(s) 441, 1686, 1753, 1860
MaeIII GTNAC 6 cut(s) 28, 306, 437, 875, 1856, 2202
MalI GATC 6 cut(s) 141, 213, 636, 1585, 1770, 2260
MboI GATC 6 cut(s) 139, 211, 634, 1583, 1768, 2258
MboII GAAGA 5 cut(s) 692, 808, 1295, 1624, 1956
MflI RGATCY 1 cut(s) 2258
MhlI GDGCHC 4 cut(s) 99, 851, 947, 1639
MlsI TGGCCA 1 cut(s) 2111
MluNI TGGCCA 1 cut(s) 2111
Mly113I GGCGCC 1 cut(s) 1410
MlyI GAGTC 1 cut(s) 788
MmeI TCCRAC 1 cut(s) 2100
Mox20I TGGCCA 1 cut(s) 2111
MroXI GAANNNNTTC 2 cut(s) 1196, 1827
MscI TGGCCA 1 cut(s) 2111
MseI TTAA 9 cut(s) 47, 615, 759, 1136, 1217, 1437, 1895, 2238, 2306
MslI CAYNNNNRTG 3 cut(s) 1317, 1473, 1594
Msp20I TGGCCA 1 cut(s) 2111
MspA1I CMGCKG 1 cut(s) 1280
MspI CCGG 2 cut(s) 749, 844
MspR9I CCNGG 5 cut(s) 117, 640, 897, 999, 1069
Mva1269I GAATGC 1 cut(s) 2133
MvaI CCWGG 5 cut(s) 117, 640, 897, 999, 1069
MvnI CGCG 2 cut(s) 629, 2326
MwoI GCNNNNNNNGC 6 cut(s) 82, 555, 897, 1801, 2054, 2152
NarI GGCGCC 1 cut(s) 1410
NcoI CCATGG 2 cut(s) 2112, 2150
NdeII GATC 6 cut(s) 139, 211, 634, 1583, 1768, 2258
NheI GCTAGC 1 cut(s) 76
NlaIV GGNNCC 7 cut(s) 911, 962, 963, 1252, 1339, 1411, 1818
NmeAIII GCCGAG 1 cut(s) 149
NmuCI GTSAC 2 cut(s) 1856, 2202
NspI RCATGY 1 cut(s) 2293
PaeI GCATGC 1 cut(s) 2293
PasI CCCWGGG 1 cut(s) 639
PceI AGGCCT 1 cut(s) 2092
PcsI WCGNNNNNNNCGW 1 cut(s) 672
PctI GAATGC 1 cut(s) 2133
PdmI GAANNNNTTC 2 cut(s) 1196, 1827
PfeI GAWTC 3 cut(s) 22, 644, 1324
PkrI GCNGC 8 cut(s) 36, 61, 82, 338, 387, 662, 744, 871
PleI GAGTC 1 cut(s) 787
PluTI GGCGCC 1 cut(s) 1413
PpsI GAGTC 1 cut(s) 787
PpuMI RGGWCCY 1 cut(s) 961
Psp1406I AACGTT 1 cut(s) 441
Psp5II RGGWCCY 1 cut(s) 961
Psp6I CCWGG 5 cut(s) 115, 638, 895, 997, 1067
PspEI GGTNACC 1 cut(s) 2202
PspFI CCCAGC 1 cut(s) 2197
PspGI CCWGG 5 cut(s) 115, 638, 895, 997, 1067
PspN4I GGNNCC 7 cut(s) 911, 962, 963, 1252, 1339, 1411, 1818
PspPI GGNCC 2 cut(s) 961, 1816
PspPPI RGGWCCY 1 cut(s) 961
PstI CTGCAG 1 cut(s) 874
PstNI CAGNNNCTG 1 cut(s) 336
PsuI RGATCY 1 cut(s) 2258
RsaI GTAC 7 cut(s) 256, 653, 1196, 1515, 1535, 2192, 2269
RsaNI GTAC 7 cut(s) 255, 652, 1195, 1514, 1534, 2191, 2268
RseI CAYNNNNRTG 3 cut(s) 1317, 1473, 1594
SalI GTCGAC 1 cut(s) 839
SaqAI TTAA 9 cut(s) 47, 615, 759, 1136, 1217, 1437, 1895, 2238, 2306
SatI GCNGC 8 cut(s) 35, 60, 81, 337, 386, 661, 743, 870
Sau3AI GATC 6 cut(s) 139, 211, 634, 1583, 1768, 2258
Sau96I GGNCC 2 cut(s) 961, 1816
ScaI AGTACT 2 cut(s) 1196, 2192
SchI GAGTC 1 cut(s) 788
ScrFI CCNGG 5 cut(s) 117, 640, 897, 999, 1069
SduI GDGCHC 4 cut(s) 99, 851, 947, 1639
SfaNI GCATC 7 cut(s) 110, 552, 1065, 1628, 1931, 2155, 2311
SfcI CTRYAG 1 cut(s) 870
SfoI GGCGCC 1 cut(s) 1411
SinI GGWCC 2 cut(s) 961, 1816
SmiMI CAYNNNNRTG 3 cut(s) 1317, 1473, 1594
SmlI CTYRAG 3 cut(s) 105, 380, 1289
SmoI CTYRAG 3 cut(s) 105, 380, 1289
SpeI ACTAGT 2 cut(s) 1522, 1789
SphI GCATGC 1 cut(s) 2293
SseBI AGGCCT 1 cut(s) 2092
SsiI CCGC 4 cut(s) 60, 135, 579, 1280
SspDI GGCGCC 1 cut(s) 1409
StuI AGGCCT 1 cut(s) 2092
StyD4I CCNGG 5 cut(s) 115, 638, 895, 997, 1067
StyI CCWWGG 4 cut(s) 166, 430, 2112, 2150
TaaI ACNGT 5 cut(s) 254, 302, 1211, 1351, 1431
TaiI ACGT 4 cut(s) 444, 1689, 1756, 1863
TaqI TCGA 6 cut(s) 218, 596, 840, 928, 936, 1681
TaqII GACCGA 1 cut(s) 548
TatI WGTACW 4 cut(s) 651, 1194, 1533, 2190
TauI GCSGC 1 cut(s) 62
TfiI GAWTC 3 cut(s) 22, 644, 1324
Tru1I TTAA 9 cut(s) 47, 615, 759, 1136, 1217, 1437, 1895, 2238, 2306
Tru9I TTAA 9 cut(s) 47, 615, 759, 1136, 1217, 1437, 1895, 2238, 2306
TscAI CASTG 2 cut(s) 893, 2281
TseFI GTSAC 2 cut(s) 1856, 2202
TseI GCWGC 7 cut(s) 34, 80, 336, 385, 660, 742, 869
Tsp45I GTSAC 2 cut(s) 1856, 2202
TspDTI ATGAA 6 cut(s) 661, 1175, 1584, 1747, 1820, 2114
TspGWI ACGGA 1 cut(s) 2271
TspRI CASTG 2 cut(s) 893, 2281
VneI GTGCAC 1 cut(s) 943
VpaK11BI GGWCC 2 cut(s) 961, 1816
XagI CCTNNNNNAGG 1 cut(s) 955
XapI RAATTY 5 cut(s) 988, 1362, 1992, 2167, 2295
XbaI TCTAGA 1 cut(s) 1765
XceI RCATGY 1 cut(s) 2293
XmaJI CCTAGG 1 cut(s) 166
XmiI GTMKAC 1 cut(s) 840
XmnI GAANNNNTTC 2 cut(s) 1196, 1827
ZrmI AGTACT 2 cut(s) 1196, 2192
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.