RchiOBHm_Chr5g0054801

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000066
Physical Location & Seq
Forward (+)
57506741 .. 57509557
2817 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ33188

Sequence Viewer

Length: 2304 bp
ATGGCTGTGCACGGGAGGATGCTAGTTATGCAACTGATCATAGCGGTGGCCATAGCAGCAGCAGCAGCTGATCGAACCCCACCTCGTCAAGCGCGACCTAACTGCCCCGACCACTGTGGTAATCTCACAATCCCATATCCTTTCGGCATTGGGAAGGGTTGTTTCTTGCGGAATGGGTTCAACCTCACTTGTAACGAAACCGCCAAACCCCCAACAACACTCTGGGGCGATGGTAGTTTCACGGTTACTAACATATTGCTTGCTGAAGGTGAGTTGGAGGTTATGAATAACATATCCCATGATTGTTATGATAAGCAGGGCAATCAAACAAAAGACATGTACAATTTCCCTAGTCTCCGGTTGCAACCTCCCTTCACCATCTCCGGGACCAGAAACAAGTTTATTGCCGTTGGCTGCGACAATTGGGCAATTTTCAAAGGGTACCGCCATTACCCTCATGACGAAGATAGGTACATCACCGGATGCATAACTGTATGTAACAATCTTGACAGTGTAGTTAACGGTTCGTGCTCTGGCATTGGGTGTTGCCAAACTGCCATCCCTAGCGGACTCAAAAATGGCACCGTCAAATTGGATAGCTATTCTCAACACACGGCGGTGTGGGACAACAATCCATGCAGCTACGCCTTCATTGTGGAAGAAGGCAGGTTCACATTCAACCCTAAAACAAGTTTTGAAGAACTCAACAACACTAAAAAACTTCCCGTGATTATTAACTGGGCTATTCATGAAGGGTCGTGTGCACTAGCTCAAAACAGCTCTGGTTATGCTTGCAAAGCAAATAGCAGCTGTGTCAATCGGACCGTCGATTACCAGACTGAACCGACTGGTTATTATTGCCAGTGCTTGCCAGGCTTCGAAGGGAACCCATATCTTCCAGATGGTTGCCAAGATATTGACGAGTGCATGGCTCCATCAAGTCCCTGCAACAATGGAATATGCCAAAATTCACCTGGAGGTTACAGCTGTTTATGTAACAAAGGTTTCAAAAATCAGGACTCAAAGATTTGCATAAAAAACACTCCCGTGTCAAAGAACATTCCCCTGAAAATTTCGATGGGTCTCAGTATAGGCTTCTTCATTTTAGTGGTTTTAATTTGGTGGACTTATTGCGGAATGAACAAAAGAAGACTAGTAAGACTCAAAGAAAAGTACTTCGAAGAAAATGGCGGCTTAACATTACAGCAACATCTCGCTAGTCATGGAGGATCTATTGAGACAGTAGAAATCTTTACTGCAGAGGAACTTCAGAAAGCCACAAACAATTACCATGCAGATGAAATCCTTGGTGAAGGAGGCTATGCAACGGTTTACAAAGGAATAGTACATGATAAGAATGTTGTTGCCATAAAGAAGCCAAAGATTGGCACTCCAACTCAAAGTGATCAGTTCGTTAATGAGATTATCGTTCTTTCTCAGATAAACCACAGAAATGTAGTAAAACTATTAGGTTGTTGTTTCGAGACCGAAGTGCCTTTACTGGTATATGAGTACATTACTCATGGCACTCTTTTCGAGCATGTATTCAATAAGGGAAAAGGATCCTCGCTTTCATGGGAACTACGATTGAAGATAGCATCAGAAACTGCAGGCGCATTAGCATACTTACACTCAGCTACTTCCACTCCAATCATACATAGAGACGTGAAAACAACGAATATATTATTGGATGACAATTACACGGCCAAAGTGTCTGATTTTGGAGCTTCGCGTTTCATTCCTCTAGATCAAACTCAACTTTCAACACTGGTTCAAGGGACGCTTGGATACTTGGATCCTGAATACTTTCATTCAAACCAACTAACTGAAAAGAGTGACGTCTATAGTTTTGGAGTTGTCCTAGCGGAGCTACTAACAAGTAGAGTTGCACTTTCTTTTGCCAGGCCTGACAAGGAGAGATGCCTAGCAAGCTTCTTTGTTTCTTCAATAGAAAACGAATGCTTGATTCAAATTCTTGATGCTGATATAGTGAATGAGGAGAACATAGAGACGGTAGAGCAAGTGGCGATTCTGGCAAAAAGATGTTTGATGATAAAAAGTGAGGAAAGGCCTACGATGAAAGACGTTGCCATGGAGTTAGAAGGGATGAGAATTATGGCAAAGCATCCATGGAAAAATGGCAATTCTGGCTTTGAGGAGACTGAGTACTTGCTCGGGTCACCAAATTCAGCGGCTTACGTGATGGATATTGGAGGTGGAGGTGCCGGTGGTTCGAGTGTTACAAATACCGGGTATGATAGCATGCAAATTCAAATGTTAATGCCCTACGATGATGGGCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK

Protein Analysis

767

Amino Acids

84.58

Weight (kDa)

5.64

Isoelectric Point (pI)

36.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 35 - 95 1.7e-12 Wall-associated receptor kinase galacturonan-binding
EGF_CA PF07645 305 - 344 1e-08 Calcium-binding EGF domain
Pkinase PF00069 430 - 696 2.2e-45 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 432 - 700 3.4e-46 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21210 AT1G21230 AT1G21240 AT1G21240 AT1G21245 AT1G21250 AT1G21270 AT1G22720 AT3G25490
fragaria_vesca FvH4_2g35051 FvH4_2g35051 FvH4_2g35060 FvH4_3g10281 FvH4_3g10285 FvH4_3g10286 FvH4_3g10311 FvH4_3g10311 FvH4_3g10320 FvH4_3g10332 FvH4_3g10340 FvH4_3g10350 FvH4_3g10360 FvH4_3g10390 FvH4_3g10412 FvH4_3g10413 FvH4_3g10461 FvH4_3g10462 FvH4_3g10470 FvH4_3g10510 FvH4_3g10520 FvH4_3g10530 FvH4_3g10550 FvH4_3g10560 FvH4_3g10611 FvH4_3g11730 FvH4_6g53140 FvH4_6g53142 FvH4_7g31880 FvH4_7g31910 FvH4_7g31920
malus_domestica MD05G1269900.v1.1 MD05G1270000.v1.1 MD05G1270200.v1.1 MD05G1270400.v1.1 MD05G1270500.v1.1 MD05G1270600.v1.1 MD05G1270800.v1.1 MD05G1270900.v1.1 MD07G1183800.v1.1 MD08G1107600.v1.1 MD10G1248500.v1.1 MD10G1249200.v1.1 MD10G1249500.v1.1 MD10G1250000.v1.1 MD10G1250500.v1.1 MD10G1250900.v1.1 MD10G1251200.v1.1 MD10G1251400.v1.1 MD13G1004600.v1.1 MD15G1088400.v1.1
prunus_persica Prupe.1G442400_v2.0.a1 Prupe.1G442500_v2.0.a1 Prupe.2G223400_v2.0.a1 Prupe.4G091900_v2.0.a1 Prupe.4G093200_v2.0.a1 Prupe.4G093300_v2.0.a1 Prupe.4G093400_v2.0.a1 Prupe.4G093500_v2.0.a1 Prupe.4G094100_v2.0.a1 Prupe.4G094200_v2.0.a1 Prupe.4G094300_v2.0.a1 Prupe.6G358300_v2.0.a1
pyrus_communis pycom08g08990 pycom10g20660 pycom10g20670 pycom10g20680 pycom10g20710 pycom10g20740 pycom13g00400 pycom15g08290
rosa_chinensis RchiOBHm_Chr1g0326861 RchiOBHm_Chr1g0326871 RchiOBHm_Chr1g0331391 RchiOBHm_Chr1g0336911 RchiOBHm_Chr1g0364001 RchiOBHm_Chr2g0174541 RchiOBHm_Chr2g0175041 RchiOBHm_Chr2g0175101 RchiOBHm_Chr2g0175121 RchiOBHm_Chr3g0486331 RchiOBHm_Chr5g0015581 RchiOBHm_Chr5g0016551 RchiOBHm_Chr5g0016561 RchiOBHm_Chr5g0016581 RchiOBHm_Chr5g0016601 RchiOBHm_Chr5g0016611 RchiOBHm_Chr5g0016641 RchiOBHm_Chr5g0016661 RchiOBHm_Chr5g0016691 RchiOBHm_Chr5g0016701 RchiOBHm_Chr5g0016731 RchiOBHm_Chr5g0016741 RchiOBHm_Chr5g0016811 RchiOBHm_Chr5g0016841 RchiOBHm_Chr5g0016871 RchiOBHm_Chr5g0016881 RchiOBHm_Chr5g0016931 RchiOBHm_Chr5g0017021 RchiOBHm_Chr5g0017041 RchiOBHm_Chr5g0017071 RchiOBHm_Chr5g0017091 RchiOBHm_Chr5g0017121 RchiOBHm_Chr5g0017141 RchiOBHm_Chr5g0017291 RchiOBHm_Chr5g0017321 RchiOBHm_Chr5g0017401 RchiOBHm_Chr5g0019001 RchiOBHm_Chr5g0019071 RchiOBHm_Chr5g0028941 RchiOBHm_Chr5g0052141 RchiOBHm_Chr5g0054721 RchiOBHm_Chr5g0054801 RchiOBHm_Chr5g0054931 RchiOBHm_Chr5g0054941 RchiOBHm_Chr6g0259291 RchiOBHm_Chr6g0292991 RchiOBHm_Chr6g0306911
rosa_laevigata RLG00000009465 RLG00000009468 RLG00000010755 RLG00000011976 RLG00000014541 RLG00000014542 RLG00000015169 RLG00000022308 RLG00000027529 RLG00000029324 RLG00000030244 RLG00000030245 RLG00000032210 RLG00000032211 RLG00000032257 RLG00000032259 RLG00000032260 RLG00000032261 RLG00000032262 RLG00000032268 RLG00000032269 RLG00000032272 RLG00000032282 RLG00000032283 RLG00000032308 RLG00000032413 RLG00000033129 RLG00000034945
rosa_multiflora Rmu_co8241929.1_g000001 Rmu_co8269227.1_g000001 Rmu_co8461259.1_g000001 Rmu_co8495385.1_g000001 Rmu_sc0000028.1_g000003 Rmu_sc0000083.1_g000009 Rmu_sc0000083.1_g000010 Rmu_sc0000083.1_g000031 Rmu_sc0000083.1_g000043 Rmu_sc0000083.1_g000046 Rmu_sc0000083.1_g000047 Rmu_sc0000563.1_g000024 Rmu_sc0000842.1_g000012 Rmu_sc0001235.1_g000001 Rmu_sc0001235.1_g000005 Rmu_sc0001235.1_g000009 Rmu_sc0001235.1_g000015 Rmu_sc0001235.1_g000016 Rmu_sc0001235.1_g000017 Rmu_sc0001473.1_g000036 Rmu_sc0001607.1_g000016 Rmu_sc0001788.1_g000006 Rmu_sc0002018.1_g000008 Rmu_sc0002028.1_g000001 Rmu_sc0002680.1_g000011 Rmu_sc0002798.1_g000023 Rmu_sc0002886.1_g000023 Rmu_sc0002989.1_g000012 Rmu_sc0003064.1_g000009 Rmu_sc0003382.1_g000006 Rmu_sc0003630.1_g000002 Rmu_sc0003630.1_g000047 Rmu_sc0003630.1_g000066 Rmu_sc0003630.1_g000067 Rmu_sc0003649.1_g000010 Rmu_sc0003688.1_g000005 Rmu_sc0003920.1_g000007 Rmu_sc0004765.1_g000007 Rmu_sc0004765.1_g000008 Rmu_sc0004920.1_g000011 Rmu_sc0005223.1_g000008 Rmu_sc0005223.1_g000010 Rmu_sc0005223.1_g000015 Rmu_sc0005223.1_g000016 Rmu_sc0005319.1_g000014 Rmu_sc0005319.1_g000015 Rmu_sc0005319.1_g000018 Rmu_sc0005319.1_g000019 Rmu_sc0005319.1_g000025 Rmu_sc0005418.1_g000007 Rmu_sc0005418.1_g000012 Rmu_sc0006106.1_g000011 Rmu_sc0006914.1_g000012 Rmu_sc0007023.1_g000001 Rmu_sc0007662.1_g000001 Rmu_sc0007920.1_g000002 Rmu_sc0007920.1_g000013 Rmu_sc0008053.1_g000016 Rmu_sc0008053.1_g000017 Rmu_sc0008053.1_g000018 Rmu_sc0008053.1_g000023 Rmu_sc0008053.1_g000050 Rmu_sc0009042.1_g000007 Rmu_sc0009042.1_g000008 Rmu_sc0010642.1_g000008 Rmu_sc0011109.1_g000002 Rmu_sc0011314.1_g000006 Rmu_sc0011974.1_g000006 Rmu_sc0012179.1_g000009 Rmu_sc0015169.1_g000001 Rmu_sc0016368.1_g000002 Rmu_sc0022659.1_g000001 Rmu_sc0027371.1_g000001 Rmu_sc0028213.1_g000001 Rmu_sc0034526.1_g000001 Rmu_sc0039366.1_g000002 Rmu_ssc0000050.1_g000084 Rmu_ssc0000050.1_g000088 Rmu_ssc0000167.1_g000017
rosa_roxburghii Rroxscaffold_1G00011340 Rroxscaffold_1G00025710 Rroxscaffold_1G00025870 Rroxscaffold_1G00026070 Rroxscaffold_1G00026170 Rroxscaffold_1G00026200 Rroxscaffold_1G00026270 Rroxscaffold_1G00050950 Rroxscaffold_1G00058900 Rroxscaffold_1G00060040 Rroxscaffold_1G00060060 Rroxscaffold_1G00060070 Rroxscaffold_1G00060080 Rroxscaffold_1G00060110 Rroxscaffold_1G00060120 Rroxscaffold_1G00060140 Rroxscaffold_1G00060170 Rroxscaffold_1G00060210 Rroxscaffold_1G00060220 Rroxscaffold_1G00060240 Rroxscaffold_1G00060250 Rroxscaffold_1G00060260 Rroxscaffold_1G00060300 Rroxscaffold_1G00060320 Rroxscaffold_1G00060330 Rroxscaffold_1G00060900 Rroxscaffold_1G00060920 Rroxscaffold_1G00073980 Rroxscaffold_2G00077560 Rroxscaffold_2G00077580 Rroxscaffold_2G00146990 Rroxscaffold_3G00236200 Rroxscaffold_3G00236210 Rroxscaffold_3G00236220 Rroxscaffold_4G00292760 Rroxscaffold_4G00315200 Rroxscaffold_4G00315240 Rroxscaffold_4G00326240 Rroxscaffold_4G00328980 Rroxscaffold_5G00341890 Rroxscaffold_7G00161350 Rroxscaffold_7G00174490 Rroxscaffold_7G00206550
rosa_rugosa Rorug01G0093000 Rorug01G0093100 Rorug01G0128800 Rorug01G0312000 Rorug01G0312000 Rorug02G0581100 Rorug03G0224600 Rorug05G0028500 Rorug05G0032700 Rorug05G0032800 Rorug05G0032900 Rorug05G0032900 Rorug05G0033000 Rorug05G0033100 Rorug05G0033300 Rorug05G0033400 Rorug05G0033900 Rorug05G0034900 Rorug05G0035000 Rorug05G0046900 Rorug05G0047500 Rorug05G0047500 Rorug05G0111600 Rorug05G0287900 Rorug05G0288700 Rorug05G0586900 Rorug06G0231700 Rorug06G0354300 Rorug06G0354400
rosa_samantha Rh1DG122200 Rh2DG686800 Rh5BG136600 Rh6AG344500 Rh6BG351900 Rh6CG359000 Rh6CG359100 Rh6CG387600 Rh6CG480500 Rh6DG344600 Rh6DG467400
rosa_wichuraiana Rw0G010250 Rw0G014820 Rw0G021400 Rw0G023100 Rw1G009450 Rw1G012100 Rw1G012180 Rw1G028360 Rw2G054260 Rw3G024370 Rw3G024420 Rw3G024470 Rw5G010850 Rw5G011010 Rw5G011020 Rw5G011050 Rw5G011080 Rw5G011120 Rw5G012300 Rw5G018470 Rw5G033830 Rw6G008930 Rw6G030070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1842
Acc36I ACCTGC 1 cut(s) 657
Acc65I GGTACC 1 cut(s) 441
AccB1I GGYRCC 3 cut(s) 441, 581, 2222
AccB7I CCANNNNNTGG 1 cut(s) 1385
AccII CGCG 2 cut(s) 94, 1732
AclWI GGATC 5 cut(s) 1237, 1557, 1570, 1790, 1803
AcoI YGGCCR 2 cut(s) 48, 1704
AcsI RAATTY 5 cut(s) 967, 1071, 1971, 2185, 2268
AcuI CTGAAG 2 cut(s) 285, 1253
AcyI GRCGYC 1 cut(s) 1839
AfaI GTAC 7 cut(s) 341, 443, 473, 1175, 1347, 1514, 2168
AfiI CCNNNNNNNGG 2 cut(s) 384, 1385
AflIII ACRYGT 1 cut(s) 336
AhlI ACTAGT 1 cut(s) 1153
AjiI CACGTC 1 cut(s) 1666
AjnI CCWGG 3 cut(s) 871, 973, 1901
AjuI GAANNNNNNNTTGG 2 cut(s) 1670, 1702
AleI CACNNNNGTG 2 cut(s) 617, 1046
Alw21I GWGCWC 3 cut(s) 12, 533, 766
Alw26I GTCTC 7 cut(s) 359, 1088, 1232, 1478, 1656, 2003, 2153
Alw44I GTGCAC 2 cut(s) 8, 762
AlwI GGATC 5 cut(s) 1237, 1557, 1570, 1790, 1803
AlwNI CAGNNNCTG 2 cut(s) 68, 1607
Ama87I CYCGRG 1 cut(s) 2174
AoxI GGCC 4 cut(s) 48, 1704, 1904, 2069
ApaLI GTGCAC 2 cut(s) 8, 762
ApeKI GCWGC 7 cut(s) 56, 59, 62, 65, 414, 639, 807
ApoI RAATTY 5 cut(s) 967, 1071, 1971, 2185, 2268
Asp700I GAANNNNTTC 2 cut(s) 176, 1806
Asp718I GGTACC 1 cut(s) 441
AspLEI GCGC 2 cut(s) 94, 1616
AspS9I GGNCC 2 cut(s) 387, 822
AsuC2I CCSGG 2 cut(s) 385, 2251
AsuHPI GGTGA 6 cut(s) 281, 367, 469, 963, 1322, 2172
AsuII TTCGAA 2 cut(s) 879, 1179
AvaI CYCGRG 1 cut(s) 2174
AvaII GGWCC 2 cut(s) 387, 822
BaeGI GKGCMC 2 cut(s) 12, 766
BalI TGGCCA 1 cut(s) 50
BamHI GGATCC 2 cut(s) 1562, 1795
BanI GGYRCC 3 cut(s) 441, 581, 2222
BarI GAAGNNNNNNTAC 2 cut(s) 1482, 1514
BbsI GAAGAC 1 cut(s) 1156
Bbv12I GWGCWC 3 cut(s) 12, 533, 766
BbvI GCAGC 7 cut(s) 68, 71, 74, 77, 401, 651, 819
BccI CCATC 8 cut(s) 224, 386, 566, 896, 943, 1072, 2197, 2288
BceAI ACGGC 3 cut(s) 392, 630, 1719
BcgI CGANNNNNNTGC 4 cut(s) 84, 118, 1516, 1550
BciT130I CCWGG 3 cut(s) 873, 975, 1903
BciVI GTATCC 1 cut(s) 1781
BclI TGATCA 2 cut(s) 36, 1405
BcnI CCSGG 2 cut(s) 385, 2251
BcoDI GTCTC 7 cut(s) 359, 1088, 1232, 1478, 1656, 2003, 2153
BcuI ACTAGT 1 cut(s) 1153
BfaI CTAG 9 cut(s) 23, 351, 564, 767, 1154, 1218, 1745, 1862, 1925
BfmI CTRYAG 3 cut(s) 1257, 1608, 1843
BfuAI ACCTGC 1 cut(s) 657
BfuI GTATCC 1 cut(s) 1781
BisI GCNGC 9 cut(s) 57, 60, 63, 66, 415, 640, 808, 1192, 2193
BlsI GCNGC 9 cut(s) 58, 61, 64, 67, 416, 641, 809, 1193, 2194
BmcAI AGTACT 2 cut(s) 1175, 2168
Bme1390I CCNGG 5 cut(s) 385, 873, 975, 1903, 2251
Bme18I GGWCC 2 cut(s) 387, 822
BmeT110I CYCGRG 1 cut(s) 2174
BmgBI CACGTC 1 cut(s) 1666
BmgT120I GGNCC 2 cut(s) 387, 822
BmiI GGNNCC 8 cut(s) 388, 443, 583, 887, 933, 1564, 1797, 2224
BmrFI CCNGG 5 cut(s) 385, 873, 975, 1903, 2251
BmrI ACTGGG 1 cut(s) 748
BmsI GCATC 6 cut(s) 9, 473, 1607, 1910, 1969, 2134
BmuI ACTGGG 1 cut(s) 748
BpiI GAAGAC 1 cut(s) 1156
BpmI CTGGAG 1 cut(s) 996
Bpu14I TTCGAA 2 cut(s) 879, 1179
BpuMI CCSGG 2 cut(s) 385, 2251
BsaAI YACGTR 1 cut(s) 2200
BsaHI GRCGYC 1 cut(s) 1839
BsaI GGTCTC 2 cut(s) 1088, 1478
BsaJI CCNNGG 3 cut(s) 1306, 2091, 2129
BsaWI WCCGGW 2 cut(s) 357, 479
BsaXI ACNNNNNCTCC 4 cut(s) 1630, 1660, 2150, 2180
Bsc4I CCNNNNNNNGG 2 cut(s) 384, 1385
Bse118I RCCGGY 1 cut(s) 2225
Bse1I ACTGG 5 cut(s) 743, 853, 862, 1506, 1773
BseBI CCWGG 3 cut(s) 873, 975, 1903
BseDI CCNNGG 3 cut(s) 1306, 2091, 2129
BseGI GGATG 6 cut(s) 24, 488, 558, 1696, 2112, 2125
BseLI CCNNNNNNNGG 2 cut(s) 384, 1385
BseMII CTCAG 4 cut(s) 1099, 1451, 1647, 2154
BseNI ACTGG 5 cut(s) 743, 853, 862, 1506, 1773
BseRI GAGGAG 2 cut(s) 2012, 2171
BseSI GKGCMC 2 cut(s) 12, 766
BseXI GCAGC 7 cut(s) 68, 71, 74, 77, 401, 651, 819
Bsh1236I CGCG 2 cut(s) 94, 1732
BshFI GGCC 4 cut(s) 50, 1706, 1906, 2071
BshNI GGYRCC 3 cut(s) 441, 581, 2222
BsiHKAI GWGCWC 3 cut(s) 12, 533, 766
BsiHKCI CYCGRG 1 cut(s) 2174
BsiSI CCGG 5 cut(s) 358, 384, 480, 2226, 2250
BslFI GGGAC 4 cut(s) 400, 638, 927, 1792
BslI CCNNNNNNNGG 2 cut(s) 384, 1385
BsmAI GTCTC 7 cut(s) 359, 1088, 1232, 1478, 1656, 2003, 2153
BsmBI CGTCTC 2 cut(s) 1656, 2003
BsmFI GGGAC 4 cut(s) 400, 638, 927, 1792
BsmI GAATGC 1 cut(s) 1964
BsnI GGCC 4 cut(s) 50, 1706, 1906, 2071
Bso31I GGTCTC 2 cut(s) 1088, 1478
BsoBI CYCGRG 1 cut(s) 2174
Bsp119I TTCGAA 2 cut(s) 879, 1179
Bsp1286I GDGCHC 3 cut(s) 12, 533, 766
Bsp1407I TGTACA 1 cut(s) 339
Bsp143I GATC 7 cut(s) 36, 70, 1229, 1405, 1562, 1747, 1795
Bsp19I CCATGG 2 cut(s) 2091, 2129
BspANI GGCC 4 cut(s) 50, 1706, 1906, 2071
BspCNI CTCAG 4 cut(s) 1098, 1450, 1646, 2155
BspFNI CGCG 2 cut(s) 94, 1732
BspHI TCATGA 2 cut(s) 457, 748
BspLI GGNNCC 8 cut(s) 388, 443, 583, 887, 933, 1564, 1797, 2224
BspMAI CTGCAG 2 cut(s) 1261, 1612
BspMI ACCTGC 1 cut(s) 657
BspPI GGATC 5 cut(s) 1237, 1557, 1570, 1790, 1803
BspT104I TTCGAA 2 cut(s) 879, 1179
BspT107I GGYRCC 3 cut(s) 441, 581, 2222
BspTNI GGTCTC 2 cut(s) 1088, 1478
BsrFI RCCGGY 1 cut(s) 2225
BsrGI TGTACA 1 cut(s) 339
BsrI ACTGG 5 cut(s) 743, 853, 862, 1506, 1773
BssAI RCCGGY 1 cut(s) 2225
BssECI CCNNGG 3 cut(s) 1306, 2091, 2129
BssMI GATC 7 cut(s) 36, 70, 1229, 1405, 1562, 1747, 1795
BssNI GRCGYC 1 cut(s) 1839
BssT1I CCWWGG 3 cut(s) 1306, 2091, 2129
Bst2UI CCWGG 3 cut(s) 873, 975, 1903
BstACI GRCGYC 1 cut(s) 1839
BstAUI TGTACA 1 cut(s) 339
BstBAI YACGTR 1 cut(s) 2200
BstBI TTCGAA 2 cut(s) 879, 1179
BstC8I GCNNGC 6 cut(s) 261, 793, 869, 1612, 1930, 2264
BstDEI CTNAG 4 cut(s) 1085, 1437, 1633, 2163
BstDSI CCRYGG 2 cut(s) 2091, 2129
BstEII GGTNACC 1 cut(s) 2178
BstF5I GGATG 6 cut(s) 24, 488, 558, 1696, 2112, 2125
BstFNI CGCG 2 cut(s) 94, 1732
BstHHI GCGC 2 cut(s) 94, 1616
BstKTI GATC 7 cut(s) 39, 73, 1232, 1408, 1565, 1750, 1798
BstMAI GTCTC 7 cut(s) 359, 1088, 1232, 1478, 1656, 2003, 2153
BstMBI GATC 7 cut(s) 36, 70, 1229, 1405, 1562, 1747, 1795
BstMWI GCNNNNNNNGC 9 cut(s) 28, 56, 62, 65, 797, 873, 1929, 2033, 2148
BstNI CCWGG 3 cut(s) 873, 975, 1903
BstNSI RCATGY 3 cut(s) 340, 1544, 2266
BstPI GGTNACC 1 cut(s) 2178
BstSCI CCNGG 5 cut(s) 383, 871, 973, 1901, 2249
BstSFI CTRYAG 3 cut(s) 1257, 1608, 1843
BstSLI GKGCMC 2 cut(s) 12, 766
BstUI CGCG 2 cut(s) 94, 1732
BstV1I GCAGC 7 cut(s) 68, 71, 74, 77, 401, 651, 819
BstV2I GAAGAC 1 cut(s) 1156
BstX2I RGATCY 3 cut(s) 1229, 1562, 1795
BstYI RGATCY 3 cut(s) 1229, 1562, 1795
BsuI GTATCC 1 cut(s) 1781
BsuRI GGCC 4 cut(s) 50, 1706, 1906, 2071
BtgI CCRYGG 2 cut(s) 2091, 2129
BtgZI GCGATG 1 cut(s) 243
BtrI CACGTC 1 cut(s) 1666
BtsCI GGATG 6 cut(s) 24, 488, 558, 1696, 2112, 2125
BtsIMutI CAGTG 4 cut(s) 112, 517, 869, 1766
BveI ACCTGC 1 cut(s) 657
Cac8I GCNNGC 6 cut(s) 261, 793, 869, 1612, 1930, 2264
CaiI CAGNNNCTG 2 cut(s) 68, 1607
CciI TCATGA 2 cut(s) 457, 748
CfoI GCGC 2 cut(s) 94, 1616
Cfr10I RCCGGY 1 cut(s) 2225
Cfr13I GGNCC 2 cut(s) 387, 822
CpoI CGGWCCG 1 cut(s) 822
CseI GACGC 1 cut(s) 1789
Csp6I GTAC 7 cut(s) 340, 442, 472, 1174, 1346, 1513, 2167
CspI CGGWCCG 1 cut(s) 822
CviQI GTAC 7 cut(s) 340, 442, 472, 1174, 1346, 1513, 2167
DdeI CTNAG 4 cut(s) 1085, 1437, 1633, 2163
DpnI GATC 7 cut(s) 38, 72, 1231, 1407, 1564, 1749, 1797
DpnII GATC 7 cut(s) 36, 70, 1229, 1405, 1562, 1747, 1795
EaeI YGGCCR 2 cut(s) 48, 1704
Eco130I CCWWGG 3 cut(s) 1306, 2091, 2129
Eco147I AGGCCT 2 cut(s) 1906, 2071
Eco31I GGTCTC 2 cut(s) 1088, 1478
Eco47I GGWCC 2 cut(s) 387, 822
Eco57I CTGAAG 2 cut(s) 285, 1253
Eco88I CYCGRG 1 cut(s) 2174
Eco91I GGTNACC 1 cut(s) 2178
EcoO65I GGTNACC 1 cut(s) 2178
EcoRII CCWGG 3 cut(s) 871, 973, 1901
EcoT14I CCWWGG 3 cut(s) 1306, 2091, 2129
EcoT22I ATGCAT 1 cut(s) 488
ErhI CCWWGG 3 cut(s) 1306, 2091, 2129
Esp3I CGTCTC 2 cut(s) 1656, 2003
FalI AAGNNNNNCTT 2 cut(s) 1767, 1799
FaqI GGGAC 4 cut(s) 400, 638, 927, 1792
FbaI TGATCA 2 cut(s) 36, 1405
Fnu4HI GCNGC 9 cut(s) 57, 60, 63, 66, 415, 640, 808, 1192, 2193
FokI GGATG 6 cut(s) 31, 495, 545, 1703, 2112, 2119
Fsp4HI GCNGC 9 cut(s) 57, 60, 63, 66, 415, 640, 808, 1192, 2193
FspBI CTAG 9 cut(s) 23, 351, 564, 767, 1154, 1218, 1745, 1862, 1925
GlaI GCGC 2 cut(s) 93, 1615
GluI GCNGC 9 cut(s) 57, 60, 63, 66, 415, 640, 808, 1192, 2193
GsuI CTGGAG 1 cut(s) 996
HaeIII GGCC 4 cut(s) 50, 1706, 1906, 2071
HapII CCGG 5 cut(s) 358, 384, 480, 2226, 2250
HgaI GACGC 1 cut(s) 1789
HhaI GCGC 2 cut(s) 94, 1616
Hin1I GRCGYC 1 cut(s) 1839
Hin6I GCGC 2 cut(s) 92, 1614
HinP1I GCGC 2 cut(s) 92, 1614
HincII GTYRAC 1 cut(s) 520
HindII GTYRAC 1 cut(s) 520
HindIII AAGCTT 1 cut(s) 1930
HinfI GANTC 5 cut(s) 570, 1019, 1161, 1966, 2029
HpaI GTTAAC 1 cut(s) 520
HpaII CCGG 5 cut(s) 358, 384, 480, 2226, 2250
HphI GGTGA 6 cut(s) 281, 367, 469, 963, 1322, 2172
Hpy166II GTNNAC 6 cut(s) 10, 520, 672, 764, 1125, 1333
Hpy188I TCNGA 5 cut(s) 822, 1272, 1440, 1603, 1717
Hpy188III TCNNGA 9 cut(s) 458, 506, 749, 899, 1016, 1483, 1745, 1799, 1976
Hpy8I GTNNAC 6 cut(s) 10, 520, 672, 764, 1125, 1333
Hpy99I CGWCG 1 cut(s) 830
HpyAV CCTTC 9 cut(s) 148, 260, 382, 656, 658, 746, 875, 1307, 2096
HpyCH4IV ACGT 4 cut(s) 1665, 1839, 2085, 2199
HpyF10VI GCNNNNNNNGC 9 cut(s) 28, 56, 62, 65, 797, 873, 1929, 2033, 2148
HpyF3I CTNAG 4 cut(s) 1085, 1437, 1633, 2163
HpySE526I ACGT 4 cut(s) 1665, 1839, 2085, 2199
Hsp92I GRCGYC 1 cut(s) 1839
HspAI GCGC 2 cut(s) 92, 1614
KpnI GGTACC 1 cut(s) 445
Ksp22I TGATCA 2 cut(s) 36, 1405
KspAI GTTAAC 1 cut(s) 520
Kzo9I GATC 7 cut(s) 36, 70, 1229, 1405, 1562, 1747, 1795
LmnI GCTCC 3 cut(s) 937, 1724, 1867
Lsp1109I GCAGC 7 cut(s) 68, 71, 74, 77, 401, 651, 819
LweI GCATC 6 cut(s) 9, 473, 1607, 1910, 1969, 2134
MaeI CTAG 9 cut(s) 23, 351, 564, 767, 1154, 1218, 1745, 1862, 1925
MaeII ACGT 4 cut(s) 1665, 1839, 2085, 2199
MaeIII GTNAC 8 cut(s) 191, 244, 497, 980, 995, 1835, 2178, 2239
MalI GATC 7 cut(s) 38, 72, 1231, 1407, 1564, 1749, 1797
MboI GATC 7 cut(s) 36, 70, 1229, 1405, 1562, 1747, 1795
MboII GAAGA 9 cut(s) 476, 671, 710, 887, 1090, 1161, 1193, 1603, 1935
MfeI CAATTG 1 cut(s) 421
MflI RGATCY 3 cut(s) 1229, 1562, 1795
MhlI GDGCHC 3 cut(s) 12, 533, 766
MlsI TGGCCA 1 cut(s) 50
MluNI TGGCCA 1 cut(s) 50
MlyI GAGTC 3 cut(s) 564, 1013, 1155
MmeI TCCRAC 2 cut(s) 255, 1418
Mox20I TGGCCA 1 cut(s) 50
Mph1103I ATGCAT 1 cut(s) 488
MroXI GAANNNNTTC 2 cut(s) 176, 1806
MscI TGGCCA 1 cut(s) 50
MseI TTAA 6 cut(s) 519, 735, 1115, 1196, 1416, 2279
MslI CAYNNNNRTG 6 cut(s) 44, 617, 1046, 1106, 1296, 1452
Msp20I TGGCCA 1 cut(s) 50
MspA1I CMGCKG 4 cut(s) 68, 810, 987, 2192
MspI CCGG 5 cut(s) 358, 384, 480, 2226, 2250
MspR9I CCNGG 5 cut(s) 385, 873, 975, 1903, 2251
MunI CAATTG 1 cut(s) 421
Mva1269I GAATGC 1 cut(s) 1964
MvaI CCWGG 3 cut(s) 873, 975, 1903
MvnI CGCG 2 cut(s) 94, 1732
MwoI GCNNNNNNNGC 9 cut(s) 28, 56, 62, 65, 797, 873, 1929, 2033, 2148
NciI CCSGG 2 cut(s) 385, 2251
NcoI CCATGG 2 cut(s) 2091, 2129
NdeII GATC 7 cut(s) 36, 70, 1229, 1405, 1562, 1747, 1795
NlaIV GGNNCC 8 cut(s) 388, 443, 583, 887, 933, 1564, 1797, 2224
NmuCI GTSAC 2 cut(s) 1835, 2178
NsiI ATGCAT 1 cut(s) 488
NspI RCATGY 3 cut(s) 340, 1544, 2266
NspV TTCGAA 2 cut(s) 879, 1179
OliI CACNNNNGTG 2 cut(s) 617, 1046
PaeI GCATGC 1 cut(s) 2266
PagI TCATGA 2 cut(s) 457, 748
PceI AGGCCT 2 cut(s) 1906, 2071
PciI ACATGT 1 cut(s) 336
PcsI WCGNNNNNNNCGW 1 cut(s) 91
PctI GAATGC 1 cut(s) 1964
PdmI GAANNNNTTC 2 cut(s) 176, 1806
PfeI GAWTC 2 cut(s) 1966, 2029
PflMI CCANNNNNTGG 1 cut(s) 1385
PfoI TCCNGGA 1 cut(s) 383
PkrI GCNGC 9 cut(s) 58, 61, 64, 67, 416, 641, 809, 1193, 2194
PleI GAGTC 3 cut(s) 564, 1013, 1155
PpsI GAGTC 3 cut(s) 564, 1013, 1155
Ppu21I YACGTR 1 cut(s) 2200
PscI ACATGT 1 cut(s) 336
Psp6I CCWGG 3 cut(s) 871, 973, 1901
PspEI GGTNACC 1 cut(s) 2178
PspGI CCWGG 3 cut(s) 871, 973, 1901
PspN4I GGNNCC 8 cut(s) 388, 443, 583, 887, 933, 1564, 1797, 2224
PspPI GGNCC 2 cut(s) 387, 822
PstI CTGCAG 2 cut(s) 1261, 1612
PstNI CAGNNNCTG 2 cut(s) 68, 1607
PsuI RGATCY 3 cut(s) 1229, 1562, 1795
PvuII CAGCTG 3 cut(s) 68, 810, 987
RsaI GTAC 7 cut(s) 341, 443, 473, 1175, 1347, 1514, 2168
RsaNI GTAC 7 cut(s) 340, 442, 472, 1174, 1346, 1513, 2167
RseI CAYNNNNRTG 6 cut(s) 44, 617, 1046, 1106, 1296, 1452
Rsr2I CGGWCCG 1 cut(s) 822
RsrII CGGWCCG 1 cut(s) 822
SaqAI TTAA 6 cut(s) 519, 735, 1115, 1196, 1416, 2279
SatI GCNGC 9 cut(s) 57, 60, 63, 66, 415, 640, 808, 1192, 2193
Sau3AI GATC 7 cut(s) 36, 70, 1229, 1405, 1562, 1747, 1795
Sau96I GGNCC 2 cut(s) 387, 822
ScaI AGTACT 2 cut(s) 1175, 2168
SchI GAGTC 3 cut(s) 564, 1013, 1155
ScrFI CCNGG 5 cut(s) 385, 873, 975, 1903, 2251
SduI GDGCHC 3 cut(s) 12, 533, 766
SfaNI GCATC 6 cut(s) 9, 473, 1607, 1910, 1969, 2134
SfcI CTRYAG 3 cut(s) 1257, 1608, 1843
SfuI TTCGAA 2 cut(s) 879, 1179
SinI GGWCC 2 cut(s) 387, 822
SmiMI CAYNNNNRTG 6 cut(s) 44, 617, 1046, 1106, 1296, 1452
SpeI ACTAGT 1 cut(s) 1153
SphI GCATGC 1 cut(s) 2266
SseBI AGGCCT 2 cut(s) 1906, 2071
SspMI CTAG 9 cut(s) 23, 351, 564, 767, 1154, 1218, 1745, 1862, 1925
StuI AGGCCT 2 cut(s) 1906, 2071
StyD4I CCNGG 5 cut(s) 383, 871, 973, 1901, 2249
StyI CCWWGG 3 cut(s) 1306, 2091, 2129
TaiI ACGT 4 cut(s) 1668, 1842, 2088, 2202
TaqI TCGA 8 cut(s) 73, 828, 879, 1076, 1179, 1482, 1536, 2234
TaqII GACCGA 1 cut(s) 1502
TatI WGTACW 5 cut(s) 339, 1173, 1345, 1512, 2166
TauI GCSGC 2 cut(s) 1194, 2195
TfiI GAWTC 2 cut(s) 1966, 2029
Tru1I TTAA 6 cut(s) 519, 735, 1115, 1196, 1416, 2279
Tru9I TTAA 6 cut(s) 519, 735, 1115, 1196, 1416, 2279
TscAI CASTG 4 cut(s) 119, 517, 869, 1773
TseFI GTSAC 2 cut(s) 1835, 2178
TseI GCWGC 7 cut(s) 56, 59, 62, 65, 414, 639, 807
Tsp45I GTSAC 2 cut(s) 1835, 2178
TspRI CASTG 4 cut(s) 119, 517, 869, 1773
Van91I CCANNNNNTGG 1 cut(s) 1385
VneI GTGCAC 2 cut(s) 8, 762
VpaK11BI GGWCC 2 cut(s) 387, 822
XapI RAATTY 5 cut(s) 967, 1071, 1971, 2185, 2268
XbaI TCTAGA 1 cut(s) 1744
XceI RCATGY 3 cut(s) 340, 1544, 2266
XcmI CCANNNNNNNNNTGG 2 cut(s) 219, 971
XmnI GAANNNNTTC 2 cut(s) 176, 1806
XspI CTAG 9 cut(s) 23, 351, 564, 767, 1154, 1218, 1745, 1862, 1925
ZraI GACGTC 1 cut(s) 1840
ZrmI AGTACT 2 cut(s) 1175, 2168
Zsp2I ATGCAT 1 cut(s) 488
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.