RLG00000032308

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000066
Physical Location & Seq
Forward (+)
13375631 .. 13381862
6232 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032308

Sequence Viewer

Length: 2361 bp
ATGGCCTCATTAAATGAGCGGATCCTTGTTACGGAGCTCTATTTGTTAATGTTAGTAGCTGCAACAACAACATCAACAACACTGCTAGCAGCAGCGCAGACCCTACCGCCGCCTCCTCAAGCCAAGTCTGGGTGCAAAGAGAAGTGTGGTGATCTCACAATTCCATATCCATTTGGCATAGGTGAAAATTGTTCCATGCGACCGGAATTCACCATCGCTTGTGACCAATCCACCACTGTATCCCCATCGGCAAACTTCACAAATGGTACCTTCACCTTTCCCATCACCATTACCAAATTTTCCCTTGATGACGGCGAGTTGCAAGTAATGCAGGATATAGCACGAAATTGTTATGATGACAAGGGTACTAATACAAACAGCAGCAGTTCACTGGAGCTGCCTGCTCCTTATATCATCTCATCCAAAAACAACTTCTTTACCCTTGGCTGCAACGTTTTATCACTGTATCAACAAGTTGTATATGAAGATTTCCAAACAGGTGTAAGCGTTGCCTTATGTGAGGATACTCTTGGGAAAGAAATACCCGAGTCATGCACCGGCTTCGGGTGTGCCCAAACTGCCATCCCAAGTGGACTGCAAATCATTTCCATTGGAATGGCTGCACTTTCCGACTACAACGCGAGTGATCCCTGGGCATCAAACTACAAATGCAGCTACGCTTTCATCGTCGAAGAAGGCAACTTCACATTCGCACCGAAAACAAGTTTTCAACTACTGAATACAACAGTTCATCAGCTTCCGGTGGCTGTTAATTGGACAATCGGGGACGAGCCATGTGAAGTAGCAAAGAACCTGACTAATTATTCGTGCAAACAGAATACTACTTGTGTCAACCGGAGTACCATCACTAATGAGACGGCTGCAGGTTACATTTGCAAGTGCCTGCCAGGCTACGAAGGGAACCCATACCTCGGTTGCCGAGATATCGATGAGTGCGCGACTTCATCAAGCGACACCTGCACGAATGGGAAGTGTGTAAATTCACCTCCAGGAAATTACTCGTGTAAATGTAATAAAGGCTTCAGAAACCAAGATCACAGGACTTGCATCCCATATTCCCGGTCAAACAACACTTCCCTAAAAATATCATTAGGTGTCAGTATAGGCTTCTTGGTTTTAGTGGTCTTGATTTTGTGGATATATTGGGAAATGAACAAAAGAAGGCTCATCAAACTCAAAAAGAAGTACTTCAAAGAAAACGGTGGCTTAACATTACAACAACAGCTCGCTAGTCATGGAGGAGCCGTGGAGACAGCAAAAATCTTTACAGCGGAAGAACTTGAGAAGGCCACAAACAATTACCATGCAGATGAGATTCTTGGTGAAGGAGCCTATGGAACAGTTTACAAAGGAATTCTACCTGATAAGAAAGTTGTTGCCATAAAAAAATCAAAAATTGGCGCCCCTACTCAAAGTGAGCAGTTCGTTAATGAGGTTATCGTTCTCTCTCAAATCAACCACAGAAATGTAGTAAGACTATTGGGTTGTTGTTTCGAGACTGAAGTGCCTTTACTAGTTTATGAGTACATTACCCATGGCACTCTTTTTGAGCATATATTCAATAAGGGAAAAGGATCACCGCTTGCATGGGAATTACGATTGAAGATAGCATCAGAAACGGCAGGGGCACTAGCTTACTTACACTCCTCTACTTCCACACCAATCATACATCGAGACGTGAAAGCAACAAATATACTATTAGATGACAACTACACGGCCAAAGTGTCCGATTTTGGAGCTTCGCGTTTCATTCCTCTAGATCAAACTGAACTAGCAACACTAGTGCAAGGGACGCTTGGCTACTTGGACCCTGAATACTTTCATTCCAATCAATTGACCGAAAAGAGTGACGTCTACAGTTTTGGAGTTGTCCTAGCAGAGCTATTAACAAGCAGAGTGGCACTTTCTTTTGCTAGACCTGAGGCAGAGAGATGCCTAGCAAGTTTCTTTGTTTCTTCAATAGAGAAAGATTGCTTCATTGAAATTCTTGACGCTAACATAGTGAATGAGGGGAACATAGACAAAGCAGAGCAAGTTGCTTATCTCGCAAAAAGATGTCTAAGGATAAAAGGGGAGGAAAGGCCTTCAATGAAAGAAGTGGCCATGGAGTTGGAGGGAATGCAAAATACAGCGAATCATCCATGGCTCGCCACCTTGCCGGAGTTCCTCTTCATCAAGGAGGATCCCCATCTCATTGGAGTTCCTTACCTCCTTAAGCCATACAGGTCAATCGAAGGCTGTCTTTTCTCCGACAGTGACAGGCTCGCTCTTTTCTCAGAAAGCTATCGAGGGACCTTCGAAACGACGATGTGTTGTCGTGAGAGGAGGAGGAGGAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK hEGF

Protein Analysis

787

Amino Acids

86.89

Weight (kDa)

5.53

Isoelectric Point (pI)

37.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 45 - 109 4.3e-09 Wall-associated receptor kinase galacturonan-binding
Pkinase PF00069 441 - 707 8.2e-46 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 443 - 711 1.7e-46 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21210 AT1G21230 AT1G21240 AT1G21240 AT1G21245 AT1G21250 AT1G21270 AT1G22720 AT3G25490
fragaria_vesca FvH4_2g35051 FvH4_2g35051 FvH4_2g35060 FvH4_3g10281 FvH4_3g10285 FvH4_3g10286 FvH4_3g10311 FvH4_3g10311 FvH4_3g10320 FvH4_3g10332 FvH4_3g10340 FvH4_3g10350 FvH4_3g10360 FvH4_3g10390 FvH4_3g10412 FvH4_3g10413 FvH4_3g10461 FvH4_3g10462 FvH4_3g10470 FvH4_3g10510 FvH4_3g10520 FvH4_3g10530 FvH4_3g10550 FvH4_3g10560 FvH4_3g10611 FvH4_3g11730 FvH4_6g53140 FvH4_6g53142 FvH4_7g31880 FvH4_7g31910 FvH4_7g31920
malus_domestica MD05G1269900.v1.1 MD05G1270000.v1.1 MD05G1270200.v1.1 MD05G1270400.v1.1 MD05G1270500.v1.1 MD05G1270600.v1.1 MD05G1270800.v1.1 MD05G1270900.v1.1 MD07G1183800.v1.1 MD08G1107600.v1.1 MD10G1248500.v1.1 MD10G1249200.v1.1 MD10G1249500.v1.1 MD10G1250000.v1.1 MD10G1250500.v1.1 MD10G1250900.v1.1 MD10G1251200.v1.1 MD10G1251400.v1.1 MD13G1004600.v1.1 MD15G1088400.v1.1
prunus_persica Prupe.1G442400_v2.0.a1 Prupe.1G442500_v2.0.a1 Prupe.2G223400_v2.0.a1 Prupe.4G091900_v2.0.a1 Prupe.4G093200_v2.0.a1 Prupe.4G093300_v2.0.a1 Prupe.4G093400_v2.0.a1 Prupe.4G093500_v2.0.a1 Prupe.4G094100_v2.0.a1 Prupe.4G094200_v2.0.a1 Prupe.4G094300_v2.0.a1 Prupe.6G358300_v2.0.a1
pyrus_communis pycom08g08990 pycom10g20660 pycom10g20670 pycom10g20680 pycom10g20710 pycom10g20740 pycom13g00400 pycom15g08290
rosa_chinensis RchiOBHm_Chr1g0326861 RchiOBHm_Chr1g0326871 RchiOBHm_Chr1g0331391 RchiOBHm_Chr1g0336911 RchiOBHm_Chr1g0364001 RchiOBHm_Chr2g0174541 RchiOBHm_Chr2g0175041 RchiOBHm_Chr2g0175101 RchiOBHm_Chr2g0175121 RchiOBHm_Chr3g0486331 RchiOBHm_Chr5g0015581 RchiOBHm_Chr5g0016551 RchiOBHm_Chr5g0016561 RchiOBHm_Chr5g0016581 RchiOBHm_Chr5g0016601 RchiOBHm_Chr5g0016611 RchiOBHm_Chr5g0016641 RchiOBHm_Chr5g0016661 RchiOBHm_Chr5g0016691 RchiOBHm_Chr5g0016701 RchiOBHm_Chr5g0016731 RchiOBHm_Chr5g0016741 RchiOBHm_Chr5g0016811 RchiOBHm_Chr5g0016841 RchiOBHm_Chr5g0016871 RchiOBHm_Chr5g0016881 RchiOBHm_Chr5g0016931 RchiOBHm_Chr5g0017021 RchiOBHm_Chr5g0017041 RchiOBHm_Chr5g0017071 RchiOBHm_Chr5g0017091 RchiOBHm_Chr5g0017121 RchiOBHm_Chr5g0017141 RchiOBHm_Chr5g0017291 RchiOBHm_Chr5g0017321 RchiOBHm_Chr5g0017401 RchiOBHm_Chr5g0019001 RchiOBHm_Chr5g0019071 RchiOBHm_Chr5g0028941 RchiOBHm_Chr5g0052141 RchiOBHm_Chr5g0054721 RchiOBHm_Chr5g0054801 RchiOBHm_Chr5g0054931 RchiOBHm_Chr5g0054941 RchiOBHm_Chr6g0259291 RchiOBHm_Chr6g0292991 RchiOBHm_Chr6g0306911
rosa_laevigata RLG00000009465 RLG00000009468 RLG00000010755 RLG00000011976 RLG00000014541 RLG00000014542 RLG00000015169 RLG00000022308 RLG00000027529 RLG00000029324 RLG00000030244 RLG00000030245 RLG00000032210 RLG00000032211 RLG00000032257 RLG00000032259 RLG00000032260 RLG00000032261 RLG00000032262 RLG00000032268 RLG00000032269 RLG00000032272 RLG00000032282 RLG00000032283 RLG00000032308 RLG00000032413 RLG00000033129 RLG00000034945
rosa_multiflora Rmu_co8241929.1_g000001 Rmu_co8269227.1_g000001 Rmu_co8461259.1_g000001 Rmu_co8495385.1_g000001 Rmu_sc0000028.1_g000003 Rmu_sc0000083.1_g000009 Rmu_sc0000083.1_g000010 Rmu_sc0000083.1_g000031 Rmu_sc0000083.1_g000043 Rmu_sc0000083.1_g000046 Rmu_sc0000083.1_g000047 Rmu_sc0000563.1_g000024 Rmu_sc0000842.1_g000012 Rmu_sc0001235.1_g000001 Rmu_sc0001235.1_g000005 Rmu_sc0001235.1_g000009 Rmu_sc0001235.1_g000015 Rmu_sc0001235.1_g000016 Rmu_sc0001235.1_g000017 Rmu_sc0001473.1_g000036 Rmu_sc0001607.1_g000016 Rmu_sc0001788.1_g000006 Rmu_sc0002018.1_g000008 Rmu_sc0002028.1_g000001 Rmu_sc0002680.1_g000011 Rmu_sc0002798.1_g000023 Rmu_sc0002886.1_g000023 Rmu_sc0002989.1_g000012 Rmu_sc0003064.1_g000009 Rmu_sc0003382.1_g000006 Rmu_sc0003630.1_g000002 Rmu_sc0003630.1_g000047 Rmu_sc0003630.1_g000066 Rmu_sc0003630.1_g000067 Rmu_sc0003649.1_g000010 Rmu_sc0003688.1_g000005 Rmu_sc0003920.1_g000007 Rmu_sc0004765.1_g000007 Rmu_sc0004765.1_g000008 Rmu_sc0004920.1_g000011 Rmu_sc0005223.1_g000008 Rmu_sc0005223.1_g000010 Rmu_sc0005223.1_g000015 Rmu_sc0005223.1_g000016 Rmu_sc0005319.1_g000014 Rmu_sc0005319.1_g000015 Rmu_sc0005319.1_g000018 Rmu_sc0005319.1_g000019 Rmu_sc0005319.1_g000025 Rmu_sc0005418.1_g000007 Rmu_sc0005418.1_g000012 Rmu_sc0006106.1_g000011 Rmu_sc0006914.1_g000012 Rmu_sc0007023.1_g000001 Rmu_sc0007662.1_g000001 Rmu_sc0007920.1_g000002 Rmu_sc0007920.1_g000013 Rmu_sc0008053.1_g000016 Rmu_sc0008053.1_g000017 Rmu_sc0008053.1_g000018 Rmu_sc0008053.1_g000023 Rmu_sc0008053.1_g000050 Rmu_sc0009042.1_g000007 Rmu_sc0009042.1_g000008 Rmu_sc0010642.1_g000008 Rmu_sc0011109.1_g000002 Rmu_sc0011314.1_g000006 Rmu_sc0011974.1_g000006 Rmu_sc0012179.1_g000009 Rmu_sc0015169.1_g000001 Rmu_sc0016368.1_g000002 Rmu_sc0022659.1_g000001 Rmu_sc0027371.1_g000001 Rmu_sc0028213.1_g000001 Rmu_sc0034526.1_g000001 Rmu_sc0039366.1_g000002 Rmu_ssc0000050.1_g000084 Rmu_ssc0000050.1_g000088 Rmu_ssc0000167.1_g000017
rosa_roxburghii Rroxscaffold_1G00011340 Rroxscaffold_1G00025710 Rroxscaffold_1G00025870 Rroxscaffold_1G00026070 Rroxscaffold_1G00026170 Rroxscaffold_1G00026200 Rroxscaffold_1G00026270 Rroxscaffold_1G00050950 Rroxscaffold_1G00058900 Rroxscaffold_1G00060040 Rroxscaffold_1G00060060 Rroxscaffold_1G00060070 Rroxscaffold_1G00060080 Rroxscaffold_1G00060110 Rroxscaffold_1G00060120 Rroxscaffold_1G00060140 Rroxscaffold_1G00060170 Rroxscaffold_1G00060210 Rroxscaffold_1G00060220 Rroxscaffold_1G00060240 Rroxscaffold_1G00060250 Rroxscaffold_1G00060260 Rroxscaffold_1G00060300 Rroxscaffold_1G00060320 Rroxscaffold_1G00060330 Rroxscaffold_1G00060900 Rroxscaffold_1G00060920 Rroxscaffold_1G00073980 Rroxscaffold_2G00077560 Rroxscaffold_2G00077580 Rroxscaffold_2G00146990 Rroxscaffold_3G00236200 Rroxscaffold_3G00236210 Rroxscaffold_3G00236220 Rroxscaffold_4G00292760 Rroxscaffold_4G00315200 Rroxscaffold_4G00315240 Rroxscaffold_4G00326240 Rroxscaffold_4G00328980 Rroxscaffold_5G00341890 Rroxscaffold_7G00161350 Rroxscaffold_7G00174490 Rroxscaffold_7G00206550
rosa_rugosa Rorug01G0093000 Rorug01G0093100 Rorug01G0128800 Rorug01G0312000 Rorug01G0312000 Rorug02G0581100 Rorug03G0224600 Rorug05G0028500 Rorug05G0032700 Rorug05G0032800 Rorug05G0032900 Rorug05G0032900 Rorug05G0033000 Rorug05G0033100 Rorug05G0033300 Rorug05G0033400 Rorug05G0033900 Rorug05G0034900 Rorug05G0035000 Rorug05G0046900 Rorug05G0047500 Rorug05G0047500 Rorug05G0111600 Rorug05G0287900 Rorug05G0288700 Rorug05G0586900 Rorug06G0231700 Rorug06G0354300 Rorug06G0354400
rosa_samantha Rh1DG122200 Rh2DG686800 Rh5BG136600 Rh6AG344500 Rh6BG351900 Rh6CG359000 Rh6CG359100 Rh6CG387600 Rh6CG480500 Rh6DG344600 Rh6DG467400
rosa_wichuraiana Rw0G010250 Rw0G014820 Rw0G021400 Rw0G023100 Rw1G009450 Rw1G012100 Rw1G012180 Rw1G028360 Rw2G054260 Rw3G024370 Rw3G024420 Rw3G024470 Rw5G010850 Rw5G011010 Rw5G011020 Rw5G011050 Rw5G011080 Rw5G011120 Rw5G012300 Rw5G018470 Rw5G033830 Rw6G008930 Rw6G030070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 986
AatII GACGTC 1 cut(s) 1875
Acc36I ACCTGC 2 cut(s) 875, 986
Acc65I GGTACC 1 cut(s) 266
AccB1I GGYRCC 2 cut(s) 266, 1421
AccBSI CCGCTC 1 cut(s) 19
AccI GTMKAC 1 cut(s) 1875
AccII CGCG 3 cut(s) 641, 959, 1765
AciI CCGC 5 cut(s) 19, 107, 110, 1292, 1601
AclI AACGTT 1 cut(s) 453
AclWI GGATC 6 cut(s) 16, 29, 641, 1603, 2198, 2211
AcoI YGGCCR 2 cut(s) 1737, 2121
AcsI RAATTY 5 cut(s) 206, 296, 1000, 1374, 2004
AcuI CTGAAG 2 cut(s) 1027, 1542
AcyI GRCGYC 2 cut(s) 1422, 1872
AfaI GTAC 5 cut(s) 268, 367, 862, 1208, 1547
AfiI CCNNNNNNNGG 4 cut(s) 31, 129, 564, 932
AflII CTTAAG 1 cut(s) 2234
AgsI TTSAA 7 cut(s) 731, 1213, 1582, 1624, 1980, 2003, 2109
AhlI ACTAGT 2 cut(s) 1534, 1801
AjiI CACGTC 1 cut(s) 1699
AjnI CCWGG 3 cut(s) 650, 907, 1009
AjuI GAANNNNNNNTTGG 2 cut(s) 416, 448
Alw21I GWGCWC 1 cut(s) 39
Alw26I GTCTC 4 cut(s) 869, 1265, 1511, 1689
AlwI GGATC 6 cut(s) 16, 29, 641, 1603, 2198, 2211
Ama87I CYCGRG 1 cut(s) 545
AoxI GGCC 5 cut(s) 3, 1308, 1737, 2102, 2121
ApeKI GCWGC 9 cut(s) 59, 89, 92, 381, 397, 447, 620, 672, 881
ApoI RAATTY 5 cut(s) 206, 296, 1000, 1374, 2004
ArsI GACNNNNNNTTYG 2 cut(s) 808, 840
Asp700I GAANNNNTTC 2 cut(s) 1208, 1839
Asp718I GGTACC 1 cut(s) 266
AspLEI GCGC 3 cut(s) 97, 959, 1424
AspS9I GGNCC 2 cut(s) 1828, 2313
AsuC2I CCSGG 1 cut(s) 1081
AsuHPI GGTGA 8 cut(s) 161, 194, 202, 265, 277, 996, 1355, 1590
AsuII TTCGAA 1 cut(s) 2319
AsuNHI GCTAGC 1 cut(s) 85
AvaI CYCGRG 1 cut(s) 545
AvaII GGWCC 2 cut(s) 1828, 2313
AxyI CCTNAGG 1 cut(s) 1941
BaeGI GKGCMC 2 cut(s) 574, 1651
BaeI ACNNNNGTAYC 4 cut(s) 222, 255, 844, 877
BalI TGGCCA 1 cut(s) 2123
BamHI GGATCC 2 cut(s) 21, 2203
BanI GGYRCC 2 cut(s) 266, 1421
BanII GRGCYC 1 cut(s) 39
BarI GAAGNNNNNNTAC 2 cut(s) 1515, 1547
BauI CACGAG 1 cut(s) 1021
Bbv12I GWGCWC 1 cut(s) 39
BbvI GCAGC 9 cut(s) 46, 101, 104, 384, 393, 434, 607, 684, 868
BccI CCATC 6 cut(s) 221, 253, 290, 590, 872, 2217
BceAI ACGGC 5 cut(s) 328, 894, 1250, 1656, 1752
BcgI CGANNNNNNTGC 2 cut(s) 544, 578
BciT130I CCWGG 3 cut(s) 652, 909, 1011
BciVI GTATCC 2 cut(s) 250, 517
BcnI CCSGG 1 cut(s) 1081
BcoDI GTCTC 4 cut(s) 869, 1265, 1511, 1689
BcuI ACTAGT 2 cut(s) 1534, 1801
BfmI CTRYAG 2 cut(s) 882, 1876
BfoI RGCGCY 1 cut(s) 1425
BfrI CTTAAG 1 cut(s) 2234
BfuAI ACCTGC 2 cut(s) 875, 986
BfuI GTATCC 2 cut(s) 250, 517
BglI GCCNNNNNGGC 1 cut(s) 909
BmcAI AGTACT 1 cut(s) 1208
Bme1390I CCNGG 4 cut(s) 652, 909, 1011, 1081
Bme18I GGWCC 2 cut(s) 1828, 2313
BmeT110I CYCGRG 1 cut(s) 545
BmgBI CACGTC 1 cut(s) 1699
BmgT120I GGNCC 2 cut(s) 1828, 2313
BmiI GGNNCC 9 cut(s) 23, 268, 923, 1264, 1351, 1423, 1830, 2205, 2314
BmrFI CCNGG 4 cut(s) 652, 909, 1011, 1081
BmsI GCATC 4 cut(s) 665, 1077, 1640, 1943
BmtI GCTAGC 1 cut(s) 89
BpmI CTGGAG 2 cut(s) 413, 993
Bpu14I TTCGAA 1 cut(s) 2319
BpuEI CTTGAG 2 cut(s) 102, 1322
BpuMI CCSGG 1 cut(s) 1081
Bsa29I ATCGAT 1 cut(s) 948
BsaBI GATNNNNATC 1 cut(s) 2208
BsaHI GRCGYC 2 cut(s) 1422, 1872
BsaJI CCNNGG 8 cut(s) 442, 650, 651, 931, 1266, 1555, 2124, 2162
BsaWI WCCGGW 3 cut(s) 202, 760, 855
BsaXI ACNNNNNCTCC 5 cut(s) 1251, 1281, 1649, 1679, 2341
Bsc4I CCNNNNNNNGG 4 cut(s) 31, 129, 564, 932
Bse118I RCCGGY 1 cut(s) 557
Bse1I ACTGG 1 cut(s) 396
Bse21I CCTNAGG 1 cut(s) 1941
Bse8I GATNNNNATC 1 cut(s) 2208
BseBI CCWGG 3 cut(s) 652, 909, 1011
BseCI ATCGAT 1 cut(s) 948
BseDI CCNNGG 8 cut(s) 442, 650, 651, 931, 1266, 1555, 2124, 2162
BseGI GGATG 4 cut(s) 419, 582, 1068, 2158
BseJI GATNNNNATC 1 cut(s) 2208
BseLI CCNNNNNNNGG 4 cut(s) 31, 129, 564, 932
BseMII CTCAG 2 cut(s) 1932, 2310
BseNI ACTGG 1 cut(s) 396
BseRI GAGGAG 4 cut(s) 105, 1275, 1657, 2359
BseSI GKGCMC 2 cut(s) 574, 1651
BseXI GCAGC 9 cut(s) 46, 101, 104, 384, 393, 434, 607, 684, 868
BsgI GTGCAG 2 cut(s) 606, 964
Bsh1236I CGCG 3 cut(s) 641, 959, 1765
Bsh1285I CGRYCG 1 cut(s) 203
BshFI GGCC 5 cut(s) 5, 1310, 1739, 2104, 2123
BshNI GGYRCC 2 cut(s) 266, 1421
BshVI ATCGAT 1 cut(s) 948
BsiEI CGRYCG 1 cut(s) 203
BsiHKAI GWGCWC 1 cut(s) 39
BsiHKCI CYCGRG 1 cut(s) 545
BsiSI CCGG 6 cut(s) 203, 558, 761, 856, 1081, 2180
BslFI GGGAC 3 cut(s) 800, 1825, 2326
BslI CCNNNNNNNGG 4 cut(s) 31, 129, 564, 932
BsmAI GTCTC 4 cut(s) 869, 1265, 1511, 1689
BsmBI CGTCTC 2 cut(s) 869, 1689
BsmFI GGGAC 3 cut(s) 800, 1825, 2326
BsmI GAATGC 1 cut(s) 2145
BsnI GGCC 5 cut(s) 5, 1310, 1739, 2104, 2123
BsoBI CYCGRG 1 cut(s) 545
Bsp119I TTCGAA 1 cut(s) 2319
Bsp1286I GDGCHC 3 cut(s) 39, 574, 1651
Bsp143I GATC 7 cut(s) 21, 151, 646, 1054, 1595, 1780, 2203
Bsp19I CCATGG 3 cut(s) 1555, 2124, 2162
BspACI CCGC 5 cut(s) 19, 107, 110, 1292, 1601
BspANI GGCC 5 cut(s) 5, 1310, 1739, 2104, 2123
BspCNI CTCAG 2 cut(s) 1933, 2309
BspDI ATCGAT 1 cut(s) 948
BspFNI CGCG 3 cut(s) 641, 959, 1765
BspLI GGNNCC 9 cut(s) 23, 268, 923, 1264, 1351, 1423, 1830, 2205, 2314
BspMAI CTGCAG 1 cut(s) 886
BspMI ACCTGC 2 cut(s) 875, 986
BspOI GCTAGC 1 cut(s) 89
BspPI GGATC 6 cut(s) 16, 29, 641, 1603, 2198, 2211
BspT104I TTCGAA 1 cut(s) 2319
BspT107I GGYRCC 2 cut(s) 266, 1421
BspTI CTTAAG 1 cut(s) 2234
BsrBI CCGCTC 1 cut(s) 19
BsrFI RCCGGY 1 cut(s) 557
BsrI ACTGG 1 cut(s) 396
BssAI RCCGGY 1 cut(s) 557
BssECI CCNNGG 8 cut(s) 442, 650, 651, 931, 1266, 1555, 2124, 2162
BssMI GATC 7 cut(s) 21, 151, 646, 1054, 1595, 1780, 2203
BssNI GRCGYC 2 cut(s) 1422, 1872
BssSI CACGAG 1 cut(s) 1021
BssT1I CCWWGG 4 cut(s) 442, 1555, 2124, 2162
Bst2BI CACGAG 1 cut(s) 1021
Bst2UI CCWGG 3 cut(s) 652, 909, 1011
Bst4CI ACNGT 7 cut(s) 238, 465, 748, 1223, 1363, 1880, 2276
Bst6I CTCTTC 1 cut(s) 2195
BstACI GRCGYC 2 cut(s) 1422, 1872
BstAFI CTTAAG 1 cut(s) 2234
BstAPI GCANNNNNTGC 1 cut(s) 328
BstBI TTCGAA 1 cut(s) 2319
BstC8I GCNNGC 7 cut(s) 87, 402, 905, 1248, 1605, 2169, 2286
BstDEI CTNAG 3 cut(s) 1941, 2081, 2296
BstDSI CCRYGG 4 cut(s) 1266, 1555, 2124, 2162
BstF5I GGATG 4 cut(s) 419, 582, 1068, 2158
BstFNI CGCG 3 cut(s) 641, 959, 1765
BstH2I RGCGCY 1 cut(s) 1425
BstHHI GCGC 3 cut(s) 97, 959, 1424
BstKTI GATC 7 cut(s) 24, 154, 649, 1057, 1598, 1783, 2206
BstMAI GTCTC 4 cut(s) 869, 1265, 1511, 1689
BstMBI GATC 7 cut(s) 21, 151, 646, 1054, 1595, 1780, 2203
BstMCI CGRYCG 1 cut(s) 203
BstMWI GCNNNNNNNGC 6 cut(s) 328, 578, 909, 978, 1813, 2066
BstNI CCWGG 3 cut(s) 652, 909, 1011
BstSCI CCNGG 4 cut(s) 650, 907, 1009, 1079
BstSFI CTRYAG 2 cut(s) 882, 1876
BstSLI GKGCMC 2 cut(s) 574, 1651
BstUI CGCG 3 cut(s) 641, 959, 1765
BstV1I GCAGC 9 cut(s) 46, 101, 104, 384, 393, 434, 607, 684, 868
BstX2I RGATCY 2 cut(s) 21, 2203
BstXI CCANNNNNNTGG 3 cut(s) 616, 2131, 2216
BstYI RGATCY 2 cut(s) 21, 2203
Bsu15I ATCGAT 1 cut(s) 948
Bsu36I CCTNAGG 1 cut(s) 1941
BsuI GTATCC 2 cut(s) 250, 517
BsuRI GGCC 5 cut(s) 5, 1310, 1739, 2104, 2123
BsuTUI ATCGAT 1 cut(s) 948
BtgI CCRYGG 4 cut(s) 1266, 1555, 2124, 2162
BtgZI GCGATG 1 cut(s) 199
BtrI CACGTC 1 cut(s) 1699
BtsCI GGATG 4 cut(s) 419, 582, 1068, 2158
BtsI GCAGTG 1 cut(s) 80
BtsIMutI CAGTG 5 cut(s) 80, 234, 389, 461, 2281
BveI ACCTGC 2 cut(s) 875, 986
Cac8I GCNNGC 7 cut(s) 87, 402, 905, 1248, 1605, 2169, 2286
CfoI GCGC 3 cut(s) 97, 959, 1424
Cfr10I RCCGGY 1 cut(s) 557
Cfr13I GGNCC 2 cut(s) 1828, 2313
ClaI ATCGAT 1 cut(s) 948
CseI GACGC 2 cut(s) 1822, 2021
Csp6I GTAC 5 cut(s) 267, 366, 861, 1207, 1546
CspCI CAANNNNNGTGG 2 cut(s) 1899, 1934
CviAII CATG 9 cut(s) 196, 552, 795, 1256, 1325, 1556, 1608, 2125, 2163
CviQI GTAC 5 cut(s) 267, 366, 861, 1207, 1546
DdeI CTNAG 3 cut(s) 1941, 2081, 2296
DinI GGCGCC 1 cut(s) 1423
DpnI GATC 7 cut(s) 23, 153, 648, 1056, 1597, 1782, 2205
DpnII GATC 7 cut(s) 21, 151, 646, 1054, 1595, 1780, 2203
EaeI YGGCCR 2 cut(s) 1737, 2121
Eam1104I CTCTTC 1 cut(s) 2195
EarI CTCTTC 1 cut(s) 2195
Ecl136II GAGCTC 1 cut(s) 37
Eco130I CCWWGG 4 cut(s) 442, 1555, 2124, 2162
Eco147I AGGCCT 1 cut(s) 2104
Eco24I GRGCYC 1 cut(s) 39
Eco32I GATATC 1 cut(s) 946
Eco47I GGWCC 2 cut(s) 1828, 2313
Eco53kI GAGCTC 1 cut(s) 37
Eco57I CTGAAG 2 cut(s) 1027, 1542
Eco81I CCTNAGG 1 cut(s) 1941
Eco88I CYCGRG 1 cut(s) 545
EcoICRI GAGCTC 1 cut(s) 37
EcoO109I RGGNCCY 1 cut(s) 2313
EcoRI GAATTC 2 cut(s) 206, 1374
EcoRII CCWGG 3 cut(s) 650, 907, 1009
EcoRV GATATC 1 cut(s) 946
EcoT14I CCWWGG 4 cut(s) 442, 1555, 2124, 2162
EcoT38I GRGCYC 1 cut(s) 39
EgeI GGCGCC 1 cut(s) 1423
EheI GGCGCC 1 cut(s) 1423
ErhI CCWWGG 4 cut(s) 442, 1555, 2124, 2162
Esp3I CGTCTC 2 cut(s) 869, 1689
FaeI CATG 9 cut(s) 199, 555, 798, 1259, 1328, 1559, 1611, 2128, 2166
FalI AAGNNNNNCTT 6 cut(s) 1193, 1225, 1800, 1832, 2247, 2279
FaqI GGGAC 3 cut(s) 800, 1825, 2326
FatI CATG 9 cut(s) 195, 551, 794, 1255, 1324, 1555, 1607, 2124, 2162
FblI GTMKAC 1 cut(s) 1875
FokI GGATG 4 cut(s) 406, 569, 1055, 2145
FriOI GRGCYC 1 cut(s) 39
GlaI GCGC 3 cut(s) 96, 958, 1423
GsuI CTGGAG 2 cut(s) 413, 993
HaeII RGCGCY 1 cut(s) 1425
HaeIII GGCC 5 cut(s) 5, 1310, 1739, 2104, 2123
HapII CCGG 6 cut(s) 203, 558, 761, 856, 1081, 2180
HgaI GACGC 2 cut(s) 1822, 2021
HhaI GCGC 3 cut(s) 97, 959, 1424
Hin1I GRCGYC 2 cut(s) 1422, 1872
Hin1II CATG 9 cut(s) 199, 555, 798, 1259, 1328, 1559, 1611, 2128, 2166
Hin6I GCGC 3 cut(s) 95, 957, 1422
HinP1I GCGC 3 cut(s) 95, 957, 1422
HincII GTYRAC 1 cut(s) 853
HindII GTYRAC 1 cut(s) 853
HinfI GANTC 3 cut(s) 548, 1336, 2155
HpaII CCGG 6 cut(s) 203, 558, 761, 856, 1081, 2180
HphI GGTGA 8 cut(s) 161, 194, 202, 265, 277, 996, 1355, 1590
Hpy166II GTNNAC 5 cut(s) 389, 593, 853, 1366, 1876
Hpy188I TCNGA 6 cut(s) 631, 1046, 1636, 1750, 2272, 2299
Hpy188III TCNNGA 6 cut(s) 1147, 1516, 1694, 1778, 2009, 2339
Hpy8I GTNNAC 5 cut(s) 389, 593, 853, 1366, 1876
Hpy99I CGWCG 2 cut(s) 692, 2329
HpyAV CCTTC 9 cut(s) 280, 689, 911, 1176, 1300, 1340, 2115, 2249, 2326
HpyCH4III ACNGT 7 cut(s) 238, 465, 748, 1223, 1363, 1880, 2276
HpyCH4IV ACGT 3 cut(s) 453, 1698, 1872
HpyF10VI GCNNNNNNNGC 6 cut(s) 328, 578, 909, 978, 1813, 2066
HpyF3I CTNAG 3 cut(s) 1941, 2081, 2296
HpySE526I ACGT 3 cut(s) 453, 1698, 1872
Hsp92I GRCGYC 2 cut(s) 1422, 1872
Hsp92II CATG 9 cut(s) 199, 555, 798, 1259, 1328, 1559, 1611, 2128, 2166
HspAI GCGC 3 cut(s) 95, 957, 1422
KasI GGCGCC 1 cut(s) 1421
KpnI GGTACC 1 cut(s) 270
Kzo9I GATC 7 cut(s) 21, 151, 646, 1054, 1595, 1780, 2203
LmnI GCTCC 6 cut(s) 34, 394, 409, 1262, 1349, 1757
Lsp1109I GCAGC 9 cut(s) 46, 101, 104, 384, 393, 434, 607, 684, 868
LweI GCATC 4 cut(s) 665, 1077, 1640, 1943
MaeII ACGT 3 cut(s) 453, 1698, 1872
MaeIII GTNAC 5 cut(s) 28, 221, 887, 1868, 2276
MalI GATC 7 cut(s) 23, 153, 648, 1056, 1597, 1782, 2205
MbiI CCGCTC 1 cut(s) 19
MboI GATC 7 cut(s) 21, 151, 646, 1054, 1595, 1780, 2203
MboII GAAGA 6 cut(s) 497, 704, 1307, 1636, 1968, 2182
MfeI CAATTG 1 cut(s) 1853
MflI RGATCY 2 cut(s) 21, 2203
MhlI GDGCHC 3 cut(s) 39, 574, 1651
MlsI TGGCCA 1 cut(s) 2123
MluNI TGGCCA 1 cut(s) 2123
Mly113I GGCGCC 1 cut(s) 1422
MlyI GAGTC 1 cut(s) 557
MmeI TCCRAC 3 cut(s) 654, 2112, 2295
Mox20I TGGCCA 1 cut(s) 2123
MroXI GAANNNNTTC 2 cut(s) 1208, 1839
MscI TGGCCA 1 cut(s) 2123
MseI TTAA 7 cut(s) 11, 47, 771, 1229, 1449, 1907, 2235
MslI CAYNNNNRTG 3 cut(s) 614, 1329, 1485
Msp20I TGGCCA 1 cut(s) 2123
MspA1I CMGCKG 1 cut(s) 1292
MspCI CTTAAG 1 cut(s) 2234
MspI CCGG 6 cut(s) 203, 558, 761, 856, 1081, 2180
MspR9I CCNGG 4 cut(s) 652, 909, 1011, 1081
MunI CAATTG 1 cut(s) 1853
Mva1269I GAATGC 1 cut(s) 2145
MvaI CCWGG 3 cut(s) 652, 909, 1011
MvnI CGCG 3 cut(s) 641, 959, 1765
MwoI GCNNNNNNNGC 6 cut(s) 328, 578, 909, 978, 1813, 2066
NarI GGCGCC 1 cut(s) 1422
NciI CCSGG 1 cut(s) 1081
NcoI CCATGG 3 cut(s) 1555, 2124, 2162
NdeII GATC 7 cut(s) 21, 151, 646, 1054, 1595, 1780, 2203
NheI GCTAGC 1 cut(s) 85
NlaIII CATG 9 cut(s) 199, 555, 798, 1259, 1328, 1559, 1611, 2128, 2166
NlaIV GGNNCC 9 cut(s) 23, 268, 923, 1264, 1351, 1423, 1830, 2205, 2314
NmeAIII GCCGAG 1 cut(s) 965
NmuCI GTSAC 3 cut(s) 221, 1868, 2276
NspV TTCGAA 1 cut(s) 2319
PaqCI CACCTGC 1 cut(s) 986
PasI CCCWGGG 1 cut(s) 651
PceI AGGCCT 1 cut(s) 2104
PcsI WCGNNNNNNNCGW 1 cut(s) 684
PctI GAATGC 1 cut(s) 2145
PdmI GAANNNNTTC 2 cut(s) 1208, 1839
PfeI GAWTC 2 cut(s) 1336, 2155
PfoI TCCNGGA 1 cut(s) 1009
PleI GAGTC 1 cut(s) 556
PluTI GGCGCC 1 cut(s) 1425
PpsI GAGTC 1 cut(s) 556
PpuMI RGGWCCY 1 cut(s) 2313
Psp124BI GAGCTC 1 cut(s) 39
Psp1406I AACGTT 1 cut(s) 453
Psp5II RGGWCCY 1 cut(s) 2313
Psp6I CCWGG 3 cut(s) 650, 907, 1009
PspGI CCWGG 3 cut(s) 650, 907, 1009
PspN4I GGNNCC 9 cut(s) 23, 268, 923, 1264, 1351, 1423, 1830, 2205, 2314
PspPI GGNCC 2 cut(s) 1828, 2313
PspPPI RGGWCCY 1 cut(s) 2313
PstI CTGCAG 1 cut(s) 886
PsuI RGATCY 2 cut(s) 21, 2203
RsaI GTAC 5 cut(s) 268, 367, 862, 1208, 1547
RsaNI GTAC 5 cut(s) 267, 366, 861, 1207, 1546
RseI CAYNNNNRTG 3 cut(s) 614, 1329, 1485
SacI GAGCTC 1 cut(s) 39
SaqAI TTAA 7 cut(s) 11, 47, 771, 1229, 1449, 1907, 2235
Sau3AI GATC 7 cut(s) 21, 151, 646, 1054, 1595, 1780, 2203
Sau96I GGNCC 2 cut(s) 1828, 2313
ScaI AGTACT 1 cut(s) 1208
SchI GAGTC 1 cut(s) 557
ScrFI CCNGG 4 cut(s) 652, 909, 1011, 1081
SduI GDGCHC 3 cut(s) 39, 574, 1651
SfaNI GCATC 4 cut(s) 665, 1077, 1640, 1943
SfcI CTRYAG 2 cut(s) 882, 1876
SfoI GGCGCC 1 cut(s) 1423
SfuI TTCGAA 1 cut(s) 2319
SinI GGWCC 2 cut(s) 1828, 2313
SmiMI CAYNNNNRTG 3 cut(s) 614, 1329, 1485
SmlI CTYRAG 3 cut(s) 117, 1301, 2234
SmoI CTYRAG 3 cut(s) 117, 1301, 2234
SpeI ACTAGT 2 cut(s) 1534, 1801
SseBI AGGCCT 1 cut(s) 2104
SsiI CCGC 5 cut(s) 19, 107, 110, 1292, 1601
SspDI GGCGCC 1 cut(s) 1421
SstI GAGCTC 1 cut(s) 39
StuI AGGCCT 1 cut(s) 2104
StyD4I CCNGG 4 cut(s) 650, 907, 1009, 1079
StyI CCWWGG 4 cut(s) 442, 1555, 2124, 2162
TaaI ACNGT 7 cut(s) 238, 465, 748, 1223, 1363, 1880, 2276
TaiI ACGT 3 cut(s) 456, 1701, 1875
TaqI TCGA 7 cut(s) 690, 948, 1515, 1693, 2253, 2308, 2319
TaqII GACCGA 1 cut(s) 1874
TatI WGTACW 2 cut(s) 1206, 1545
TauI GCSGC 1 cut(s) 112
TfiI GAWTC 2 cut(s) 1336, 2155
Tru1I TTAA 7 cut(s) 11, 47, 771, 1229, 1449, 1907, 2235
Tru9I TTAA 7 cut(s) 11, 47, 771, 1229, 1449, 1907, 2235
TscAI CASTG 5 cut(s) 87, 241, 396, 468, 2281
TseFI GTSAC 3 cut(s) 221, 1868, 2276
TseI GCWGC 9 cut(s) 59, 89, 92, 381, 397, 447, 620, 672, 881
Tsp45I GTSAC 3 cut(s) 221, 1868, 2276
TspGWI ACGGA 1 cut(s) 47
TspRI CASTG 5 cut(s) 87, 241, 396, 468, 2281
Vha464I CTTAAG 1 cut(s) 2234
VpaK11BI GGWCC 2 cut(s) 1828, 2313
XapI RAATTY 5 cut(s) 206, 296, 1000, 1374, 2004
XbaI TCTAGA 1 cut(s) 1777
XmiI GTMKAC 1 cut(s) 1875
XmnI GAANNNNTTC 2 cut(s) 1208, 1839
ZraI GACGTC 1 cut(s) 1873
ZrmI AGTACT 1 cut(s) 1208
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.