RLG00000032282

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000066
Physical Location & Seq
Reverse (-)
13042226 .. 13045755
3530 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032282

Sequence Viewer

Length: 2211 bp
ATGGCCTTACAGGGGAGGCTGCTTGGTGTACTATTCATCTCAATGGTACTGGGGGCATCATCTGATCAAGCCTTGCCTCCTCAAGCCCTGCCTGGATGCCTCAGCCGGTGTGGTAATCTCACAATCCCGTATCCCTTCGGCGTAGGGGATGGTTGTTATCTGCGACAAAGATTCAACATCACTTGTAACGAATCTGCCCATCCCCCAACAGCACTTTTGGCGGATGGTAGTATCATTGCTACTAACATATCCCTGGGTGAAGGTGAGCTGCAATTTTGGAATACCATAGGCCATGATTGTTATGATAAACAGGGCAATCAAACGGGGAGCATGCGCAATTCCCCTTCGCTTCAGTTGAATCCTCCTTTCACCATCTCTGGTACCAAAAACAACTTGATCGCTGTTGGCTGTGACACTTCCGCAATTTTCAAAGGAAACTTTCCTTACCCTTATGATGAAGCGAGCCATGCAGCTACGCCTTCATTGTGGAAGAAGGCGAGTTCACATTCTTCCCGAATTCAAGTTTTCAAGAACTGTCCAAGAATCAACAACTTCCCGTCATTCTTAATTGGGAAATTCAGGATGGGCTGTGCCGAAGCTCAAAAGAGGCATGATTATGCATGCAAGGCAAATAGCAGGTGTGTGAACCGGACCATCGACTTCCAGACTGAACCGTCTGGTTACTATTGCCAGTGCTTGCCAGGCTTTGAAGGGAACCCGTATCTCCCAGACGGTTGCCGTGATGTTAATGAGTGCATAGCTCCAACAAACCCCTGCAACAATGGAAAGTGCGCAAATTTACCTGGAAGTTACAGATGTTTATGTTGGAAAGGTTTCAGAAACAAAGACCCGACGACTTGCATGCCAAACAATCCAACATCAAAGATCAAGTCCCTGAAAATTTCGTTGGGTGTCAGTTTAGGCTTCTCAGTTTTGCTAGTTGTAACAATATGGATTCACTGGGAAATGAACAAAAGAAGGATTATCAAACTCAAAGAGAAGTACTTCAAAGAAAATGGCGGCTTAATGTTGCAGCAACAACTCGCTCGTCATGGAGGAAGGGTGGATACAACAAGAATATTTACTTCAGAAGAACTTGAGAAAGCCACAAACAATTACCATGAAAGTAGAATCCTTGGTGAAGGTGGCTATGGAACTGTTTATAAAGGAACATTACCGGATAACACAATGGTGGCCATAAAGAAGTCCAAAGTTGCTGCCCGAACTCAAAATGATCAGTTTGTTAATGAGGTGATTTTTCTTTCTCAAATAAACCACAGAAATGTGGTAAGACTATTAGGTTGTTGTTTTGAGACAGAAGTGCCTTTACTAGTATATGAGTACATCACCCATGGCACTCTTTTTGAGCATTTATTCAACAATAAAAATGGTAAAAGATCATCACTTTCGTGGGAGTTACGATTGAAGATAGCATCGGAAACTGCAGGAGCACTAGCATACTTGCACTCTTCCACTTCCACACCAATAGTACACCGGGATGTGAAAGCAACAAATATACTGTTGAATGAAAATTATACGGCCAAAGTGTCGGATTTTGGAGCTTCGCGTTTCATTCCTCTGGATCAAACTCAAGTAGCAACATTAGTGCAAGGCACAATGGGATACTTAGATCCCGAATATATGCAATCAAACACGCTAACAGAAAAGAGTGATGTCTATAGCTTTGGAGTTGTCCTAGCAGAGCTATTAACGAGCAAAGTGGCGGTTTCTTTTGCTAGGCCCGAGTCAGAAAGAAGCGTAGCATATTTGTTTGTTTCCTCAATTGAAAAACAATGCCTGATTCAAATTCTTGATGCTGACATGGTGAATGAGGGAAATGTCGAGATGGTTGAGCAGGTGGCAAATTTGGCAAAAATATGTCTAAGGGTAAAAGGGGAGGAAAGGCCTATGATGAAGGAAGTGGCCATGGAGTTAGAGGGAATGAGAATTACGGCAAAGCATCCATGGAAAAATGCTGATTTCTATCCAGAGGAGACTGAGTGCTTGTTGGGTTCTCCTAATTCCAAGTCTTACATTGAAGGTAAAGGTGAAGTTAGTTCTAGTGGTTTAGTGAGTGGTGGCACAACAAAGGAGTGTGAAAAGGAGATAGATGAGGAGTTGGCTATCAATCACGGGGAAATCTCCGGTGTCTATGACTCTGAGAGGAGGAATAGTCACTCATGGACCCCGGAAATCTCTTCCTTGATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK

Protein Analysis

737

Amino Acids

81.58

Weight (kDa)

7.18

Isoelectric Point (pI)

38.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 33 - 89 5.3e-09 Wall-associated receptor kinase galacturonan-binding
EGF_CA PF07645 248 - 287 4e-10 Calcium-binding EGF domain
Pkinase PF00069 373 - 641 3.1e-46 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 376 - 645 2.2e-48 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21210 AT1G21230 AT1G21240 AT1G21240 AT1G21245 AT1G21250 AT1G21270 AT1G22720 AT3G25490
fragaria_vesca FvH4_2g35051 FvH4_2g35051 FvH4_2g35060 FvH4_3g10281 FvH4_3g10285 FvH4_3g10286 FvH4_3g10311 FvH4_3g10311 FvH4_3g10320 FvH4_3g10332 FvH4_3g10340 FvH4_3g10350 FvH4_3g10360 FvH4_3g10390 FvH4_3g10412 FvH4_3g10413 FvH4_3g10461 FvH4_3g10462 FvH4_3g10470 FvH4_3g10510 FvH4_3g10520 FvH4_3g10530 FvH4_3g10550 FvH4_3g10560 FvH4_3g10611 FvH4_3g11730 FvH4_6g53140 FvH4_6g53142 FvH4_7g31880 FvH4_7g31910 FvH4_7g31920
malus_domestica MD05G1269900.v1.1 MD05G1270000.v1.1 MD05G1270200.v1.1 MD05G1270400.v1.1 MD05G1270500.v1.1 MD05G1270600.v1.1 MD05G1270800.v1.1 MD05G1270900.v1.1 MD07G1183800.v1.1 MD08G1107600.v1.1 MD10G1248500.v1.1 MD10G1249200.v1.1 MD10G1249500.v1.1 MD10G1250000.v1.1 MD10G1250500.v1.1 MD10G1250900.v1.1 MD10G1251200.v1.1 MD10G1251400.v1.1 MD13G1004600.v1.1 MD15G1088400.v1.1
prunus_persica Prupe.1G442400_v2.0.a1 Prupe.1G442500_v2.0.a1 Prupe.2G223400_v2.0.a1 Prupe.4G091900_v2.0.a1 Prupe.4G093200_v2.0.a1 Prupe.4G093300_v2.0.a1 Prupe.4G093400_v2.0.a1 Prupe.4G093500_v2.0.a1 Prupe.4G094100_v2.0.a1 Prupe.4G094200_v2.0.a1 Prupe.4G094300_v2.0.a1 Prupe.6G358300_v2.0.a1
pyrus_communis pycom08g08990 pycom10g20660 pycom10g20670 pycom10g20680 pycom10g20710 pycom10g20740 pycom13g00400 pycom15g08290
rosa_chinensis RchiOBHm_Chr1g0326861 RchiOBHm_Chr1g0326871 RchiOBHm_Chr1g0331391 RchiOBHm_Chr1g0336911 RchiOBHm_Chr1g0364001 RchiOBHm_Chr2g0174541 RchiOBHm_Chr2g0175041 RchiOBHm_Chr2g0175101 RchiOBHm_Chr2g0175121 RchiOBHm_Chr3g0486331 RchiOBHm_Chr5g0015581 RchiOBHm_Chr5g0016551 RchiOBHm_Chr5g0016561 RchiOBHm_Chr5g0016581 RchiOBHm_Chr5g0016601 RchiOBHm_Chr5g0016611 RchiOBHm_Chr5g0016641 RchiOBHm_Chr5g0016661 RchiOBHm_Chr5g0016691 RchiOBHm_Chr5g0016701 RchiOBHm_Chr5g0016731 RchiOBHm_Chr5g0016741 RchiOBHm_Chr5g0016811 RchiOBHm_Chr5g0016841 RchiOBHm_Chr5g0016871 RchiOBHm_Chr5g0016881 RchiOBHm_Chr5g0016931 RchiOBHm_Chr5g0017021 RchiOBHm_Chr5g0017041 RchiOBHm_Chr5g0017071 RchiOBHm_Chr5g0017091 RchiOBHm_Chr5g0017121 RchiOBHm_Chr5g0017141 RchiOBHm_Chr5g0017291 RchiOBHm_Chr5g0017321 RchiOBHm_Chr5g0017401 RchiOBHm_Chr5g0019001 RchiOBHm_Chr5g0019071 RchiOBHm_Chr5g0028941 RchiOBHm_Chr5g0052141 RchiOBHm_Chr5g0054721 RchiOBHm_Chr5g0054801 RchiOBHm_Chr5g0054931 RchiOBHm_Chr5g0054941 RchiOBHm_Chr6g0259291 RchiOBHm_Chr6g0292991 RchiOBHm_Chr6g0306911
rosa_laevigata RLG00000009465 RLG00000009468 RLG00000010755 RLG00000011976 RLG00000014541 RLG00000014542 RLG00000015169 RLG00000022308 RLG00000027529 RLG00000029324 RLG00000030244 RLG00000030245 RLG00000032210 RLG00000032211 RLG00000032257 RLG00000032259 RLG00000032260 RLG00000032261 RLG00000032262 RLG00000032268 RLG00000032269 RLG00000032272 RLG00000032282 RLG00000032283 RLG00000032308 RLG00000032413 RLG00000033129 RLG00000034945
rosa_multiflora Rmu_co8241929.1_g000001 Rmu_co8269227.1_g000001 Rmu_co8461259.1_g000001 Rmu_co8495385.1_g000001 Rmu_sc0000028.1_g000003 Rmu_sc0000083.1_g000009 Rmu_sc0000083.1_g000010 Rmu_sc0000083.1_g000031 Rmu_sc0000083.1_g000043 Rmu_sc0000083.1_g000046 Rmu_sc0000083.1_g000047 Rmu_sc0000563.1_g000024 Rmu_sc0000842.1_g000012 Rmu_sc0001235.1_g000001 Rmu_sc0001235.1_g000005 Rmu_sc0001235.1_g000009 Rmu_sc0001235.1_g000015 Rmu_sc0001235.1_g000016 Rmu_sc0001235.1_g000017 Rmu_sc0001473.1_g000036 Rmu_sc0001607.1_g000016 Rmu_sc0001788.1_g000006 Rmu_sc0002018.1_g000008 Rmu_sc0002028.1_g000001 Rmu_sc0002680.1_g000011 Rmu_sc0002798.1_g000023 Rmu_sc0002886.1_g000023 Rmu_sc0002989.1_g000012 Rmu_sc0003064.1_g000009 Rmu_sc0003382.1_g000006 Rmu_sc0003630.1_g000002 Rmu_sc0003630.1_g000047 Rmu_sc0003630.1_g000066 Rmu_sc0003630.1_g000067 Rmu_sc0003649.1_g000010 Rmu_sc0003688.1_g000005 Rmu_sc0003920.1_g000007 Rmu_sc0004765.1_g000007 Rmu_sc0004765.1_g000008 Rmu_sc0004920.1_g000011 Rmu_sc0005223.1_g000008 Rmu_sc0005223.1_g000010 Rmu_sc0005223.1_g000015 Rmu_sc0005223.1_g000016 Rmu_sc0005319.1_g000014 Rmu_sc0005319.1_g000015 Rmu_sc0005319.1_g000018 Rmu_sc0005319.1_g000019 Rmu_sc0005319.1_g000025 Rmu_sc0005418.1_g000007 Rmu_sc0005418.1_g000012 Rmu_sc0006106.1_g000011 Rmu_sc0006914.1_g000012 Rmu_sc0007023.1_g000001 Rmu_sc0007662.1_g000001 Rmu_sc0007920.1_g000002 Rmu_sc0007920.1_g000013 Rmu_sc0008053.1_g000016 Rmu_sc0008053.1_g000017 Rmu_sc0008053.1_g000018 Rmu_sc0008053.1_g000023 Rmu_sc0008053.1_g000050 Rmu_sc0009042.1_g000007 Rmu_sc0009042.1_g000008 Rmu_sc0010642.1_g000008 Rmu_sc0011109.1_g000002 Rmu_sc0011314.1_g000006 Rmu_sc0011974.1_g000006 Rmu_sc0012179.1_g000009 Rmu_sc0015169.1_g000001 Rmu_sc0016368.1_g000002 Rmu_sc0022659.1_g000001 Rmu_sc0027371.1_g000001 Rmu_sc0028213.1_g000001 Rmu_sc0034526.1_g000001 Rmu_sc0039366.1_g000002 Rmu_ssc0000050.1_g000084 Rmu_ssc0000050.1_g000088 Rmu_ssc0000167.1_g000017
rosa_roxburghii Rroxscaffold_1G00011340 Rroxscaffold_1G00025710 Rroxscaffold_1G00025870 Rroxscaffold_1G00026070 Rroxscaffold_1G00026170 Rroxscaffold_1G00026200 Rroxscaffold_1G00026270 Rroxscaffold_1G00050950 Rroxscaffold_1G00058900 Rroxscaffold_1G00060040 Rroxscaffold_1G00060060 Rroxscaffold_1G00060070 Rroxscaffold_1G00060080 Rroxscaffold_1G00060110 Rroxscaffold_1G00060120 Rroxscaffold_1G00060140 Rroxscaffold_1G00060170 Rroxscaffold_1G00060210 Rroxscaffold_1G00060220 Rroxscaffold_1G00060240 Rroxscaffold_1G00060250 Rroxscaffold_1G00060260 Rroxscaffold_1G00060300 Rroxscaffold_1G00060320 Rroxscaffold_1G00060330 Rroxscaffold_1G00060900 Rroxscaffold_1G00060920 Rroxscaffold_1G00073980 Rroxscaffold_2G00077560 Rroxscaffold_2G00077580 Rroxscaffold_2G00146990 Rroxscaffold_3G00236200 Rroxscaffold_3G00236210 Rroxscaffold_3G00236220 Rroxscaffold_4G00292760 Rroxscaffold_4G00315200 Rroxscaffold_4G00315240 Rroxscaffold_4G00326240 Rroxscaffold_4G00328980 Rroxscaffold_5G00341890 Rroxscaffold_7G00161350 Rroxscaffold_7G00174490 Rroxscaffold_7G00206550
rosa_rugosa Rorug01G0093000 Rorug01G0093100 Rorug01G0128800 Rorug01G0312000 Rorug01G0312000 Rorug02G0581100 Rorug03G0224600 Rorug05G0028500 Rorug05G0032700 Rorug05G0032800 Rorug05G0032900 Rorug05G0032900 Rorug05G0033000 Rorug05G0033100 Rorug05G0033300 Rorug05G0033400 Rorug05G0033900 Rorug05G0034900 Rorug05G0035000 Rorug05G0046900 Rorug05G0047500 Rorug05G0047500 Rorug05G0111600 Rorug05G0287900 Rorug05G0288700 Rorug05G0586900 Rorug06G0231700 Rorug06G0354300 Rorug06G0354400
rosa_samantha Rh1DG122200 Rh2DG686800 Rh5BG136600 Rh6AG344500 Rh6BG351900 Rh6CG359000 Rh6CG359100 Rh6CG387600 Rh6CG480500 Rh6DG344600 Rh6DG467400
rosa_wichuraiana Rw0G010250 Rw0G014820 Rw0G021400 Rw0G023100 Rw1G009450 Rw1G012100 Rw1G012180 Rw1G028360 Rw2G054260 Rw3G024370 Rw3G024420 Rw3G024470 Rw5G010850 Rw5G011010 Rw5G011020 Rw5G011050 Rw5G011080 Rw5G011120 Rw5G012300 Rw5G018470 Rw5G033830 Rw6G008930 Rw6G030070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1164
AarI CACCTGC 2 cut(s) 627, 1846
AasI GACNNNNNNGTC 1 cut(s) 673
Acc16I TGCGCA 2 cut(s) 335, 793
Acc36I ACCTGC 2 cut(s) 627, 1846
Acc65I GGTACC 1 cut(s) 380
AccB1I GGYRCC 1 cut(s) 380
AccII CGCG 1 cut(s) 1567
AciI CCGC 4 cut(s) 221, 420, 1020, 1724
AclWI GGATC 2 cut(s) 1590, 1625
AcoI YGGCCR 3 cut(s) 1194, 1539, 1923
AcsI RAATTY 6 cut(s) 516, 575, 796, 900, 1806, 1864
AcuI CTGAAG 2 cut(s) 335, 1071
AfaI GTAC 6 cut(s) 30, 48, 382, 1004, 1343, 1491
AfiI CCNNNNNNNGG 1 cut(s) 12
AhlI ACTAGT 1 cut(s) 1330
AjnI CCWGG 4 cut(s) 91, 252, 700, 802
AjuI GAANNNNNNNTTGG 2 cut(s) 890, 922
AleI CACNNNNGTG 2 cut(s) 1190, 1408
AluBI AGCT 7 cut(s) 268, 473, 599, 761, 1562, 1683, 1705
AluI AGCT 7 cut(s) 268, 473, 599, 761, 1562, 1683, 1705
Alw21I GWGCWC 1 cut(s) 1453
Alw26I GTCTC 2 cut(s) 1307, 1988
AlwI GGATC 2 cut(s) 1590, 1625
Ama87I CYCGRG 1 cut(s) 1742
AoxI GGCC 7 cut(s) 3, 289, 1194, 1539, 1739, 1904, 1923
ApeKI GCWGC 5 cut(s) 19, 268, 470, 1033, 1217
ApoI RAATTY 6 cut(s) 516, 575, 796, 900, 1806, 1864
ArsI GACNNNNNNTTYG 2 cut(s) 875, 907
Asp700I GAANNNNTTC 2 cut(s) 833, 1004
Asp718I GGTACC 1 cut(s) 380
AspLEI GCGC 2 cut(s) 336, 794
AspS9I GGNCC 3 cut(s) 651, 1740, 2184
AsuC2I CCSGG 2 cut(s) 1496, 2189
AsuHPI GGTGA 8 cut(s) 269, 275, 361, 1151, 1264, 1339, 1837, 2060
AvaI CYCGRG 1 cut(s) 1742
AvaII GGWCC 2 cut(s) 651, 2184
BalI TGGCCA 2 cut(s) 1196, 1925
BanI GGYRCC 1 cut(s) 380
BarI GAAGNNNNNNTAC 4 cut(s) 1051, 1083, 1311, 1343
Bbv12I GWGCWC 1 cut(s) 1453
BbvCI CCTCAGC 1 cut(s) 101
BbvI GCAGC 5 cut(s) 6, 255, 482, 1045, 1204
BccI CCATC 7 cut(s) 143, 207, 218, 380, 577, 662, 1840
BceAI ACGGC 3 cut(s) 723, 1554, 1968
BcgI CGANNNNNNTGC 2 cut(s) 844, 878
BciT130I CCWGG 4 cut(s) 93, 254, 702, 804
BciVI GTATCC 3 cut(s) 141, 1060, 1616
BclI TGATCA 2 cut(s) 64, 1234
BcnI CCSGG 2 cut(s) 1496, 2189
BcoDI GTCTC 2 cut(s) 1307, 1988
BcuI ACTAGT 1 cut(s) 1330
BfaI CTAG 6 cut(s) 938, 1331, 1454, 1697, 1737, 2061
BfmI CTRYAG 2 cut(s) 1443, 1678
BfuAI ACCTGC 2 cut(s) 627, 1846
BfuI GTATCC 3 cut(s) 141, 1060, 1616
BisI GCNGC 6 cut(s) 20, 269, 471, 1021, 1034, 1218
BlsI GCNGC 6 cut(s) 21, 270, 472, 1022, 1035, 1219
BmcAI AGTACT 1 cut(s) 1004
Bme1390I CCNGG 6 cut(s) 93, 254, 702, 804, 1496, 2189
Bme18I GGWCC 2 cut(s) 651, 2184
BmeT110I CYCGRG 1 cut(s) 1742
BmgT120I GGNCC 3 cut(s) 651, 1740, 2184
BmiI GGNNCC 3 cut(s) 382, 716, 2186
BmrFI CCNGG 6 cut(s) 93, 254, 702, 804, 1496, 2189
BmrI ACTGGG 2 cut(s) 59, 970
BmsI GCATC 5 cut(s) 65, 86, 1442, 1804, 1969
BmuI ACTGGG 2 cut(s) 59, 970
Bpu10I CCTNAGC 1 cut(s) 101
BpuEI CTTGAG 3 cut(s) 66, 1118, 1575
BpuMI CCSGG 2 cut(s) 1496, 2189
BsaBI GATNNNNATC 1 cut(s) 1983
BsaJI CCNNGG 7 cut(s) 252, 253, 1135, 1351, 1926, 1964, 2187
BsaWI WCCGGW 3 cut(s) 648, 1177, 2144
BsaXI ACNNNNNCTCC 6 cut(s) 1047, 1077, 1985, 2015, 2158, 2188
Bsc4I CCNNNNNNNGG 1 cut(s) 12
Bse118I RCCGGY 1 cut(s) 105
Bse1I ACTGG 3 cut(s) 54, 691, 965
Bse3DI GCAATG 1 cut(s) 234
Bse8I GATNNNNATC 1 cut(s) 1983
BseBI CCWGG 4 cut(s) 93, 254, 702, 804
BseDI CCNNGG 7 cut(s) 252, 253, 1135, 1351, 1926, 1964, 2187
BseGI GGATG 7 cut(s) 101, 154, 199, 229, 588, 1504, 1960
BseJI GATNNNNATC 1 cut(s) 1983
BseLI CCNNNNNNNGG 1 cut(s) 12
BseMI GCAATG 1 cut(s) 234
BseMII CTCAG 4 cut(s) 115, 942, 1989, 2151
BseNI ACTGG 3 cut(s) 54, 691, 965
BseRI GAGGAG 4 cut(s) 69, 2006, 2129, 2179
BseXI GCAGC 5 cut(s) 6, 255, 482, 1045, 1204
Bsh1236I CGCG 1 cut(s) 1567
BshFI GGCC 7 cut(s) 5, 291, 1196, 1541, 1741, 1906, 1925
BshNI GGYRCC 1 cut(s) 380
BsiHKAI GWGCWC 1 cut(s) 1453
BsiHKCI CYCGRG 1 cut(s) 1742
BsiSI CCGG 6 cut(s) 106, 649, 1178, 1495, 2145, 2189
BslFI GGGAC 1 cut(s) 877
BslI CCNNNNNNNGG 1 cut(s) 12
BsmAI GTCTC 2 cut(s) 1307, 1988
BsmFI GGGAC 1 cut(s) 877
BsnI GGCC 7 cut(s) 5, 291, 1196, 1541, 1741, 1906, 1925
BsoBI CYCGRG 1 cut(s) 1742
Bsp1286I GDGCHC 1 cut(s) 1453
Bsp143I GATC 7 cut(s) 64, 396, 885, 1234, 1397, 1582, 1630
Bsp19I CCATGG 3 cut(s) 1351, 1926, 1964
BspACI CCGC 4 cut(s) 221, 420, 1020, 1724
BspANI GGCC 7 cut(s) 5, 291, 1196, 1541, 1741, 1906, 1925
BspCNI CTCAG 4 cut(s) 114, 941, 1990, 2152
BspFNI CGCG 1 cut(s) 1567
BspLI GGNNCC 3 cut(s) 382, 716, 2186
BspMAI CTGCAG 1 cut(s) 1447
BspMI ACCTGC 2 cut(s) 627, 1846
BspPI GGATC 2 cut(s) 1590, 1625
BspT107I GGYRCC 1 cut(s) 380
BsrDI GCAATG 1 cut(s) 234
BsrFI RCCGGY 1 cut(s) 105
BsrI ACTGG 3 cut(s) 54, 691, 965
BssAI RCCGGY 1 cut(s) 105
BssECI CCNNGG 7 cut(s) 252, 253, 1135, 1351, 1926, 1964, 2187
BssMI GATC 7 cut(s) 64, 396, 885, 1234, 1397, 1582, 1630
BssT1I CCWWGG 4 cut(s) 1135, 1351, 1926, 1964
Bst2UI CCWGG 4 cut(s) 93, 254, 702, 804
Bst4CI ACNGT 5 cut(s) 536, 675, 734, 1159, 1521
Bst6I CTCTTC 2 cut(s) 1474, 2203
BstC8I GCNNGC 5 cut(s) 332, 463, 622, 698, 863
BstDEI CTNAG 6 cut(s) 101, 928, 1627, 1883, 1998, 2160
BstDSI CCRYGG 3 cut(s) 1351, 1926, 1964
BstF5I GGATG 7 cut(s) 101, 154, 199, 229, 588, 1504, 1960
BstFNI CGCG 1 cut(s) 1567
BstHHI GCGC 2 cut(s) 336, 794
BstKTI GATC 7 cut(s) 67, 399, 888, 1237, 1400, 1585, 1633
BstMAI GTCTC 2 cut(s) 1307, 1988
BstMBI GATC 7 cut(s) 64, 396, 885, 1234, 1397, 1582, 1630
BstMWI GCNNNNNNNGC 5 cut(s) 218, 467, 626, 702, 1868
BstNI CCWGG 4 cut(s) 93, 254, 702, 804
BstNSI RCATGY 3 cut(s) 334, 624, 865
BstSCI CCNGG 6 cut(s) 91, 252, 700, 802, 1494, 2187
BstSFI CTRYAG 2 cut(s) 1443, 1678
BstUI CGCG 1 cut(s) 1567
BstV1I GCAGC 5 cut(s) 6, 255, 482, 1045, 1204
BstX2I RGATCY 1 cut(s) 1630
BstYI RGATCY 1 cut(s) 1630
BsuI GTATCC 3 cut(s) 141, 1060, 1616
BsuRI GGCC 7 cut(s) 5, 291, 1196, 1541, 1741, 1906, 1925
BtgI CCRYGG 3 cut(s) 1351, 1926, 1964
BtsCI GGATG 7 cut(s) 101, 154, 199, 229, 588, 1504, 1960
BtsIMutI CAGTG 2 cut(s) 698, 958
BveI ACCTGC 2 cut(s) 627, 1846
Cac8I GCNNGC 5 cut(s) 332, 463, 622, 698, 863
CfoI GCGC 2 cut(s) 336, 794
Cfr10I RCCGGY 1 cut(s) 105
Cfr13I GGNCC 3 cut(s) 651, 1740, 2184
Csp6I GTAC 6 cut(s) 29, 47, 381, 1003, 1342, 1490
CviQI GTAC 6 cut(s) 29, 47, 381, 1003, 1342, 1490
DdeI CTNAG 6 cut(s) 101, 928, 1627, 1883, 1998, 2160
DpnI GATC 7 cut(s) 66, 398, 887, 1236, 1399, 1584, 1632
DpnII GATC 7 cut(s) 64, 396, 885, 1234, 1397, 1582, 1630
DrdI GACNNNNNNGTC 1 cut(s) 673
DseDI GACNNNNNNGTC 1 cut(s) 673
EaeI YGGCCR 3 cut(s) 1194, 1539, 1923
Eam1104I CTCTTC 2 cut(s) 1474, 2203
EarI CTCTTC 2 cut(s) 1474, 2203
EciI GGCGGA 1 cut(s) 236
Eco130I CCWWGG 4 cut(s) 1135, 1351, 1926, 1964
Eco147I AGGCCT 1 cut(s) 1906
Eco47I GGWCC 2 cut(s) 651, 2184
Eco57I CTGAAG 2 cut(s) 335, 1071
Eco88I CYCGRG 1 cut(s) 1742
EcoRI GAATTC 1 cut(s) 516
EcoRII CCWGG 4 cut(s) 91, 252, 700, 802
EcoT14I CCWWGG 4 cut(s) 1135, 1351, 1926, 1964
EcoT22I ATGCAT 1 cut(s) 622
ErhI CCWWGG 4 cut(s) 1135, 1351, 1926, 1964
FaqI GGGAC 1 cut(s) 877
FbaI TGATCA 2 cut(s) 64, 1234
Fnu4HI GCNGC 6 cut(s) 20, 269, 471, 1021, 1034, 1218
FokI GGATG 7 cut(s) 108, 161, 186, 236, 595, 1511, 1947
Fsp4HI GCNGC 6 cut(s) 20, 269, 471, 1021, 1034, 1218
FspBI CTAG 6 cut(s) 938, 1331, 1454, 1697, 1737, 2061
FspI TGCGCA 2 cut(s) 335, 793
GlaI GCGC 2 cut(s) 335, 793
GluI GCNGC 6 cut(s) 20, 269, 471, 1021, 1034, 1218
HaeIII GGCC 7 cut(s) 5, 291, 1196, 1541, 1741, 1906, 1925
HapII CCGG 6 cut(s) 106, 649, 1178, 1495, 2145, 2189
HhaI GCGC 2 cut(s) 336, 794
Hin6I GCGC 2 cut(s) 334, 792
HinP1I GCGC 2 cut(s) 334, 792
HinfI GANTC 9 cut(s) 171, 191, 358, 543, 955, 1131, 1745, 1801, 2156
HpaII CCGG 6 cut(s) 106, 649, 1178, 1495, 2145, 2189
HphI GGTGA 8 cut(s) 269, 275, 361, 1151, 1264, 1339, 1837, 2060
Hpy166II GTNNAC 4 cut(s) 29, 503, 646, 1492
Hpy188I TCNGA 7 cut(s) 64, 839, 1090, 1438, 1552, 1750, 2161
Hpy188III TCNNGA 9 cut(s) 513, 529, 580, 664, 1580, 1634, 1811, 1843, 1988
Hpy8I GTNNAC 4 cut(s) 29, 503, 646, 1492
Hpy99I CGWCG 1 cut(s) 856
HpyCH4III ACNGT 5 cut(s) 536, 675, 734, 1159, 1521
HpyF10VI GCNNNNNNNGC 5 cut(s) 218, 467, 626, 702, 1868
HpyF3I CTNAG 6 cut(s) 101, 928, 1627, 1883, 1998, 2160
HspAI GCGC 2 cut(s) 334, 792
KpnI GGTACC 1 cut(s) 384
Ksp22I TGATCA 2 cut(s) 64, 1234
Kzo9I GATC 7 cut(s) 64, 396, 885, 1234, 1397, 1582, 1630
LmnI GCTCC 4 cut(s) 327, 766, 1448, 1559
Lsp1109I GCAGC 5 cut(s) 6, 255, 482, 1045, 1204
LweI GCATC 5 cut(s) 65, 86, 1442, 1804, 1969
MaeI CTAG 6 cut(s) 938, 1331, 1454, 1697, 1737, 2061
MaeIII GTNAC 7 cut(s) 185, 410, 680, 809, 943, 1416, 2174
MalI GATC 7 cut(s) 66, 398, 887, 1236, 1399, 1584, 1632
MboI GATC 7 cut(s) 64, 396, 885, 1234, 1397, 1582, 1630
MboII GAAGA 6 cut(s) 501, 502, 1103, 1438, 1461, 2190
MfeI CAATTG 1 cut(s) 1782
MflI RGATCY 1 cut(s) 1630
MhlI GDGCHC 1 cut(s) 1453
MlsI TGGCCA 2 cut(s) 1196, 1925
MluNI TGGCCA 2 cut(s) 1196, 1925
MlyI GAGTC 2 cut(s) 1754, 2150
MmeI TCCRAC 4 cut(s) 788, 806, 899, 1530
Mox20I TGGCCA 2 cut(s) 1196, 1925
Mph1103I ATGCAT 1 cut(s) 622
MroXI GAANNNNTTC 2 cut(s) 833, 1004
MscI TGGCCA 2 cut(s) 1196, 1925
MseI TTAA 5 cut(s) 566, 747, 1025, 1245, 1709
MslI CAYNNNNRTG 6 cut(s) 41, 615, 1190, 1281, 1408, 1497
Msp20I TGGCCA 2 cut(s) 1196, 1925
MspI CCGG 6 cut(s) 106, 649, 1178, 1495, 2145, 2189
MspR9I CCNGG 6 cut(s) 93, 254, 702, 804, 1496, 2189
MunI CAATTG 1 cut(s) 1782
MvaI CCWGG 4 cut(s) 93, 254, 702, 804
MvnI CGCG 1 cut(s) 1567
MwoI GCNNNNNNNGC 5 cut(s) 218, 467, 626, 702, 1868
NciI CCSGG 2 cut(s) 1496, 2189
NcoI CCATGG 3 cut(s) 1351, 1926, 1964
NdeII GATC 7 cut(s) 64, 396, 885, 1234, 1397, 1582, 1630
NlaIV GGNNCC 3 cut(s) 382, 716, 2186
NmuCI GTSAC 2 cut(s) 410, 2174
NsbI TGCGCA 2 cut(s) 335, 793
NsiI ATGCAT 1 cut(s) 622
NspI RCATGY 3 cut(s) 334, 624, 865
OliI CACNNNNGTG 2 cut(s) 1190, 1408
PaeI GCATGC 3 cut(s) 334, 624, 865
PaqCI CACCTGC 2 cut(s) 627, 1846
PasI CCCWGGG 1 cut(s) 253
PceI AGGCCT 1 cut(s) 1906
PdmI GAANNNNTTC 2 cut(s) 833, 1004
PfeI GAWTC 7 cut(s) 171, 191, 358, 543, 955, 1131, 1801
PkrI GCNGC 6 cut(s) 21, 270, 472, 1022, 1035, 1219
PleI GAGTC 2 cut(s) 1753, 2150
PpsI GAGTC 2 cut(s) 1753, 2150
PsiI TTATAA 1 cut(s) 1164
Psp6I CCWGG 4 cut(s) 91, 252, 700, 802
PspGI CCWGG 4 cut(s) 91, 252, 700, 802
PspN4I GGNNCC 3 cut(s) 382, 716, 2186
PspPI GGNCC 3 cut(s) 651, 1740, 2184
PstI CTGCAG 1 cut(s) 1447
PsuI RGATCY 1 cut(s) 1630
RsaI GTAC 6 cut(s) 30, 48, 382, 1004, 1343, 1491
RsaNI GTAC 6 cut(s) 29, 47, 381, 1003, 1342, 1490
RseI CAYNNNNRTG 6 cut(s) 41, 615, 1190, 1281, 1408, 1497
SaqAI TTAA 5 cut(s) 566, 747, 1025, 1245, 1709
SatI GCNGC 6 cut(s) 20, 269, 471, 1021, 1034, 1218
Sau3AI GATC 7 cut(s) 64, 396, 885, 1234, 1397, 1582, 1630
Sau96I GGNCC 3 cut(s) 651, 1740, 2184
ScaI AGTACT 1 cut(s) 1004
SchI GAGTC 2 cut(s) 1754, 2150
ScrFI CCNGG 6 cut(s) 93, 254, 702, 804, 1496, 2189
SduI GDGCHC 1 cut(s) 1453
SfaNI GCATC 5 cut(s) 65, 86, 1442, 1804, 1969
SfcI CTRYAG 2 cut(s) 1443, 1678
SinI GGWCC 2 cut(s) 651, 2184
SmiMI CAYNNNNRTG 6 cut(s) 41, 615, 1190, 1281, 1408, 1497
SmlI CTYRAG 3 cut(s) 81, 1097, 1590
SmoI CTYRAG 3 cut(s) 81, 1097, 1590
SpeI ACTAGT 1 cut(s) 1330
SphI GCATGC 3 cut(s) 334, 624, 865
SseBI AGGCCT 1 cut(s) 1906
SsiI CCGC 4 cut(s) 221, 420, 1020, 1724
SspI AATATT 1 cut(s) 1080
SspMI CTAG 6 cut(s) 938, 1331, 1454, 1697, 1737, 2061
StuI AGGCCT 1 cut(s) 1906
StyD4I CCNGG 6 cut(s) 91, 252, 700, 802, 1494, 2187
StyI CCWWGG 4 cut(s) 1135, 1351, 1926, 1964
TaaI ACNGT 5 cut(s) 536, 675, 734, 1159, 1521
TaqI TCGA 2 cut(s) 657, 1842
TatI WGTACW 4 cut(s) 28, 1002, 1341, 1489
TauI GCSGC 1 cut(s) 1023
TfiI GAWTC 7 cut(s) 171, 191, 358, 543, 955, 1131, 1801
Tru1I TTAA 5 cut(s) 566, 747, 1025, 1245, 1709
Tru9I TTAA 5 cut(s) 566, 747, 1025, 1245, 1709
TscAI CASTG 2 cut(s) 698, 965
TseFI GTSAC 2 cut(s) 410, 2174
TseI GCWGC 5 cut(s) 19, 268, 470, 1033, 1217
Tsp45I GTSAC 2 cut(s) 410, 2174
TspDTI ATGAA 8 cut(s) 25, 471, 471, 983, 1137, 1542, 1561, 1928
TspRI CASTG 2 cut(s) 698, 965
VpaK11BI GGWCC 2 cut(s) 651, 2184
XapI RAATTY 6 cut(s) 516, 575, 796, 900, 1806, 1864
XceI RCATGY 3 cut(s) 334, 624, 865
XmnI GAANNNNTTC 2 cut(s) 833, 1004
XspI CTAG 6 cut(s) 938, 1331, 1454, 1697, 1737, 2061
ZrmI AGTACT 1 cut(s) 1004
Zsp2I ATGCAT 1 cut(s) 622
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.