MD05G1270500.v1.1

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000066
Physical Location & Seq
Forward (+)
40631948 .. 40634144
2197 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1270500.v1.1.491

Sequence Viewer

Length: 1749 bp
ATGACCTTCTGCGAGAGCTTAGGGAGCATTCGTGAGTCTTGCTCCGGCATTGGGTGCTGCCAGACTTCAATCCCAAGTGGGCTTCAAGTTCGTACCGTGATGATGAGTAGCTACTCCAATCATACGCTTATATGGGACTTTAATCCATGTAGCTATTCCTTCATTGTGGAAGAAGGCCAGTTCACATTTTCTAATAGAAGTTTCCAAGAGCTCGAAAACACAAGCCGGCTTCCGATGGTTCTCAATTGGGCAATTGGGGATGAAGAATGTGATGCAGCTCAAAAGAGGGAGGACTATGCATGCAAGGGAAATAGCACTTGTGTCAGCCCCGTCAATCTGTCCGGCTACTTTTGTCAGTGCTTGCCAGGTTACGAAGGAAATCCGTACCCCCCAGAGGGTTGTCATGATATCGACGAGTGTGAGATTTCAAACCGTTGCAGCGCAGGAGCGTGTATAAATTTACCTGGAAATTATTCTTGTGTCTGTCCAAAAGGGTTCAAAGGCGACGGAATGAAAACTGGAACTGGAACTGGTTGCAGCAAAGACAATGGAAGCAATCTTTCCAAAAGCATTCGCTTGCTTATTATTTCATTGGCTGTGACAGTAGCCTCCTTGATTTTCCTGGTGGGAGGTTCATGGACCTACTGGGGAATGAAGAAAAACAACTACATCAAACTCAGAGAAAAGTATTTCAAAGAGAATGGTGGCATGTTGTTACAACAACGACCCGCGAGTCGCCAAGGAGGATTGGTGGAGACAACAAAAATCTTTACAGCAGAAGAACTTGACAAGGCTACAGGCTACTACGATGACAGTAGAGTCCTCGGCGAAGGAGGCTACGGTACAGTTTACAAAGGAATACTACCAGATAATGAAGTGGTTGCCATAAAAAAGTCGAAAATCGGAGCTTCAATCCAAAGTGAGCAGTTTATAAACGAAGTTATTGTTCTTTCTCAAGTCAACCACAGAAATGTGGTGAAGCTACTGGGTTGCTGTTTGGAGACACAAGTGCCTTTACTAGTTTACGAGTTCATCACCAGGGGAACTCTTTTTTATCATCTTCATAACGTAAAGAGCAAAGAATCGTCGCTTTCGTGGGAGTTACGACTGAAGATCGCAGCTGAAACTGCCGGAGCACTAGCGTACTTGCACTCCTCCACTTCCATGCCAATCATACACCGAGATGTGAAAACAACAAATATATTATTAGACGAAAATTACACAGCAAAAGTGTCGGACTTCGGAGCTTCGCGATTGATTCCTCTAGATCAAGCTCAAATCACAACTCTAGTGCAAGGGACGCTAGGGTACTTGGACCCTGAATACTTCCATTCAAACCAGCTGACAGAAAAGAGTGACGTCTACAGTTTCGGAGTTGTTCTTGTGGAGCTGCTAACAAGAAAAGTGGCACTGTCATTTACTAGGCCTGAAGAAGAGAGAAACCCGGCAAACTTCTTCGTTTGTTCCGTGGAACAGGGTCGCTTGACTCAAATTCTCGATGACGACATAGTCACCGACAGAAATCTTGAGACACTGAAAAGAGTGGCTAATCTTGCAAGTAGATGCTTGAGGGTGAAAAGGGAGGAAAGGCCTAGCATGAAAAAGGTAGCAATGGAGCTAGAAGGATACAGACTCTGGCGAAGCATCCACGGAGAACTGCTGATTTCAGTCCAGAGGACACTGATTACTTGCTCGGGCCGCCTTCAATGGCTTATGCTAGAGGTGATAGTTGTTCCAGTACTAGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK

Protein Analysis

583

Amino Acids

64.8

Weight (kDa)

6.21

Isoelectric Point (pI)

34.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EGF_CA PF07645 136 - 171 9.6e-07 Calcium-binding EGF domain
EGF PF00008 140 - 169 6.5e-06 EGF-like domain
Pkinase PF00069 269 - 536 1.9e-47 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 273 - 540 1.4e-47 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21210 AT1G21230 AT1G21240 AT1G21240 AT1G21245 AT1G21250 AT1G21270 AT1G22720 AT3G25490
fragaria_vesca FvH4_2g35051 FvH4_2g35051 FvH4_2g35060 FvH4_3g10281 FvH4_3g10285 FvH4_3g10286 FvH4_3g10311 FvH4_3g10311 FvH4_3g10320 FvH4_3g10332 FvH4_3g10340 FvH4_3g10350 FvH4_3g10360 FvH4_3g10390 FvH4_3g10412 FvH4_3g10413 FvH4_3g10461 FvH4_3g10462 FvH4_3g10470 FvH4_3g10510 FvH4_3g10520 FvH4_3g10530 FvH4_3g10550 FvH4_3g10560 FvH4_3g10611 FvH4_3g11730 FvH4_6g53140 FvH4_6g53142 FvH4_7g31880 FvH4_7g31910 FvH4_7g31920
malus_domestica MD05G1269900.v1.1 MD05G1270000.v1.1 MD05G1270200.v1.1 MD05G1270400.v1.1 MD05G1270500.v1.1 MD05G1270600.v1.1 MD05G1270800.v1.1 MD05G1270900.v1.1 MD07G1183800.v1.1 MD08G1107600.v1.1 MD10G1248500.v1.1 MD10G1249200.v1.1 MD10G1249500.v1.1 MD10G1250000.v1.1 MD10G1250500.v1.1 MD10G1250900.v1.1 MD10G1251200.v1.1 MD10G1251400.v1.1 MD13G1004600.v1.1 MD15G1088400.v1.1
prunus_persica Prupe.1G442400_v2.0.a1 Prupe.1G442500_v2.0.a1 Prupe.2G223400_v2.0.a1 Prupe.4G091900_v2.0.a1 Prupe.4G093200_v2.0.a1 Prupe.4G093300_v2.0.a1 Prupe.4G093400_v2.0.a1 Prupe.4G093500_v2.0.a1 Prupe.4G094100_v2.0.a1 Prupe.4G094200_v2.0.a1 Prupe.4G094300_v2.0.a1 Prupe.6G358300_v2.0.a1
pyrus_communis pycom08g08990 pycom10g20660 pycom10g20670 pycom10g20680 pycom10g20710 pycom10g20740 pycom13g00400 pycom15g08290
rosa_chinensis RchiOBHm_Chr1g0326861 RchiOBHm_Chr1g0326871 RchiOBHm_Chr1g0331391 RchiOBHm_Chr1g0336911 RchiOBHm_Chr1g0364001 RchiOBHm_Chr2g0174541 RchiOBHm_Chr2g0175041 RchiOBHm_Chr2g0175101 RchiOBHm_Chr2g0175121 RchiOBHm_Chr3g0486331 RchiOBHm_Chr5g0015581 RchiOBHm_Chr5g0016551 RchiOBHm_Chr5g0016561 RchiOBHm_Chr5g0016581 RchiOBHm_Chr5g0016601 RchiOBHm_Chr5g0016611 RchiOBHm_Chr5g0016641 RchiOBHm_Chr5g0016661 RchiOBHm_Chr5g0016691 RchiOBHm_Chr5g0016701 RchiOBHm_Chr5g0016731 RchiOBHm_Chr5g0016741 RchiOBHm_Chr5g0016811 RchiOBHm_Chr5g0016841 RchiOBHm_Chr5g0016871 RchiOBHm_Chr5g0016881 RchiOBHm_Chr5g0016931 RchiOBHm_Chr5g0017021 RchiOBHm_Chr5g0017041 RchiOBHm_Chr5g0017071 RchiOBHm_Chr5g0017091 RchiOBHm_Chr5g0017121 RchiOBHm_Chr5g0017141 RchiOBHm_Chr5g0017291 RchiOBHm_Chr5g0017321 RchiOBHm_Chr5g0017401 RchiOBHm_Chr5g0019001 RchiOBHm_Chr5g0019071 RchiOBHm_Chr5g0028941 RchiOBHm_Chr5g0052141 RchiOBHm_Chr5g0054721 RchiOBHm_Chr5g0054801 RchiOBHm_Chr5g0054931 RchiOBHm_Chr5g0054941 RchiOBHm_Chr6g0259291 RchiOBHm_Chr6g0292991 RchiOBHm_Chr6g0306911
rosa_laevigata RLG00000009465 RLG00000009468 RLG00000010755 RLG00000011976 RLG00000014541 RLG00000014542 RLG00000015169 RLG00000022308 RLG00000027529 RLG00000029324 RLG00000030244 RLG00000030245 RLG00000032210 RLG00000032211 RLG00000032257 RLG00000032259 RLG00000032260 RLG00000032261 RLG00000032262 RLG00000032268 RLG00000032269 RLG00000032272 RLG00000032282 RLG00000032283 RLG00000032308 RLG00000032413 RLG00000033129 RLG00000034945
rosa_multiflora Rmu_co8241929.1_g000001 Rmu_co8269227.1_g000001 Rmu_co8461259.1_g000001 Rmu_co8495385.1_g000001 Rmu_sc0000028.1_g000003 Rmu_sc0000083.1_g000009 Rmu_sc0000083.1_g000010 Rmu_sc0000083.1_g000031 Rmu_sc0000083.1_g000043 Rmu_sc0000083.1_g000046 Rmu_sc0000083.1_g000047 Rmu_sc0000563.1_g000024 Rmu_sc0000842.1_g000012 Rmu_sc0001235.1_g000001 Rmu_sc0001235.1_g000005 Rmu_sc0001235.1_g000009 Rmu_sc0001235.1_g000015 Rmu_sc0001235.1_g000016 Rmu_sc0001235.1_g000017 Rmu_sc0001473.1_g000036 Rmu_sc0001607.1_g000016 Rmu_sc0001788.1_g000006 Rmu_sc0002018.1_g000008 Rmu_sc0002028.1_g000001 Rmu_sc0002680.1_g000011 Rmu_sc0002798.1_g000023 Rmu_sc0002886.1_g000023 Rmu_sc0002989.1_g000012 Rmu_sc0003064.1_g000009 Rmu_sc0003382.1_g000006 Rmu_sc0003630.1_g000002 Rmu_sc0003630.1_g000047 Rmu_sc0003630.1_g000066 Rmu_sc0003630.1_g000067 Rmu_sc0003649.1_g000010 Rmu_sc0003688.1_g000005 Rmu_sc0003920.1_g000007 Rmu_sc0004765.1_g000007 Rmu_sc0004765.1_g000008 Rmu_sc0004920.1_g000011 Rmu_sc0005223.1_g000008 Rmu_sc0005223.1_g000010 Rmu_sc0005223.1_g000015 Rmu_sc0005223.1_g000016 Rmu_sc0005319.1_g000014 Rmu_sc0005319.1_g000015 Rmu_sc0005319.1_g000018 Rmu_sc0005319.1_g000019 Rmu_sc0005319.1_g000025 Rmu_sc0005418.1_g000007 Rmu_sc0005418.1_g000012 Rmu_sc0006106.1_g000011 Rmu_sc0006914.1_g000012 Rmu_sc0007023.1_g000001 Rmu_sc0007662.1_g000001 Rmu_sc0007920.1_g000002 Rmu_sc0007920.1_g000013 Rmu_sc0008053.1_g000016 Rmu_sc0008053.1_g000017 Rmu_sc0008053.1_g000018 Rmu_sc0008053.1_g000023 Rmu_sc0008053.1_g000050 Rmu_sc0009042.1_g000007 Rmu_sc0009042.1_g000008 Rmu_sc0010642.1_g000008 Rmu_sc0011109.1_g000002 Rmu_sc0011314.1_g000006 Rmu_sc0011974.1_g000006 Rmu_sc0012179.1_g000009 Rmu_sc0015169.1_g000001 Rmu_sc0016368.1_g000002 Rmu_sc0022659.1_g000001 Rmu_sc0027371.1_g000001 Rmu_sc0028213.1_g000001 Rmu_sc0034526.1_g000001 Rmu_sc0039366.1_g000002 Rmu_ssc0000050.1_g000084 Rmu_ssc0000050.1_g000088 Rmu_ssc0000167.1_g000017
rosa_roxburghii Rroxscaffold_1G00011340 Rroxscaffold_1G00025710 Rroxscaffold_1G00025870 Rroxscaffold_1G00026070 Rroxscaffold_1G00026170 Rroxscaffold_1G00026200 Rroxscaffold_1G00026270 Rroxscaffold_1G00050950 Rroxscaffold_1G00058900 Rroxscaffold_1G00060040 Rroxscaffold_1G00060060 Rroxscaffold_1G00060070 Rroxscaffold_1G00060080 Rroxscaffold_1G00060110 Rroxscaffold_1G00060120 Rroxscaffold_1G00060140 Rroxscaffold_1G00060170 Rroxscaffold_1G00060210 Rroxscaffold_1G00060220 Rroxscaffold_1G00060240 Rroxscaffold_1G00060250 Rroxscaffold_1G00060260 Rroxscaffold_1G00060300 Rroxscaffold_1G00060320 Rroxscaffold_1G00060330 Rroxscaffold_1G00060900 Rroxscaffold_1G00060920 Rroxscaffold_1G00073980 Rroxscaffold_2G00077560 Rroxscaffold_2G00077580 Rroxscaffold_2G00146990 Rroxscaffold_3G00236200 Rroxscaffold_3G00236210 Rroxscaffold_3G00236220 Rroxscaffold_4G00292760 Rroxscaffold_4G00315200 Rroxscaffold_4G00315240 Rroxscaffold_4G00326240 Rroxscaffold_4G00328980 Rroxscaffold_5G00341890 Rroxscaffold_7G00161350 Rroxscaffold_7G00174490 Rroxscaffold_7G00206550
rosa_rugosa Rorug01G0093000 Rorug01G0093100 Rorug01G0128800 Rorug01G0312000 Rorug01G0312000 Rorug02G0581100 Rorug03G0224600 Rorug05G0028500 Rorug05G0032700 Rorug05G0032800 Rorug05G0032900 Rorug05G0032900 Rorug05G0033000 Rorug05G0033100 Rorug05G0033300 Rorug05G0033400 Rorug05G0033900 Rorug05G0034900 Rorug05G0035000 Rorug05G0046900 Rorug05G0047500 Rorug05G0047500 Rorug05G0111600 Rorug05G0287900 Rorug05G0288700 Rorug05G0586900 Rorug06G0231700 Rorug06G0354300 Rorug06G0354400
rosa_samantha Rh1DG122200 Rh2DG686800 Rh5BG136600 Rh6AG344500 Rh6BG351900 Rh6CG359000 Rh6CG359100 Rh6CG387600 Rh6CG480500 Rh6DG344600 Rh6DG467400
rosa_wichuraiana Rw0G010250 Rw0G014820 Rw0G021400 Rw0G023100 Rw1G009450 Rw1G012100 Rw1G012180 Rw1G028360 Rw2G054260 Rw3G024370 Rw3G024420 Rw3G024470 Rw5G010850 Rw5G011010 Rw5G011020 Rw5G011050 Rw5G011080 Rw5G011120 Rw5G012300 Rw5G018470 Rw5G033830 Rw6G008930 Rw6G030070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 932
AasI GACNNNNNNGTC 3 cut(s) 732, 818, 1508
AatII GACGTC 1 cut(s) 1362
AccI GTMKAC 1 cut(s) 1362
AccII CGCG 2 cut(s) 731, 1252
AciI CCGC 2 cut(s) 729, 1699
AcsI RAATTY 2 cut(s) 457, 1491
AcuI CTGAAG 2 cut(s) 1130, 1449
AcyI GRCGYC 1 cut(s) 1359
AfaI GTAC 6 cut(s) 94, 386, 844, 1145, 1310, 1740
AfiI CCNNNNNNNGG 3 cut(s) 51, 394, 395
AgsI TTSAA 8 cut(s) 69, 86, 429, 499, 694, 912, 1335, 1706
AhlI ACTAGT 1 cut(s) 1018
AjnI CCWGG 4 cut(s) 364, 463, 621, 1037
Alw21I GWGCWC 2 cut(s) 213, 1138
Alw26I GTCTC 3 cut(s) 749, 995, 1523
AlwNI CAGNNNCTG 1 cut(s) 1635
Ama87I CYCGRG 1 cut(s) 1693
AoxI GGCC 4 cut(s) 175, 1424, 1589, 1696
ApeKI GCWGC 6 cut(s) 57, 275, 438, 537, 1118, 1390
ApoI RAATTY 2 cut(s) 457, 1491
Asp700I GAANNNNTTC 1 cut(s) 472
AspLEI GCGC 1 cut(s) 443
AspS9I GGNCC 3 cut(s) 639, 1315, 1696
AsuC2I CCSGG 1 cut(s) 1445
AsuHPI GGTGA 5 cut(s) 988, 1027, 1504, 1585, 1735
AvaI CYCGRG 1 cut(s) 1693
AvaII GGWCC 2 cut(s) 639, 1315
BanII GRGCYC 1 cut(s) 213
Bbv12I GWGCWC 2 cut(s) 213, 1138
BbvI GCAGC 6 cut(s) 44, 287, 450, 549, 1130, 1377
BccI CCATC 1 cut(s) 229
BcgI CGANNNNNNTGC 2 cut(s) 1215, 1249
BciT130I CCWGG 4 cut(s) 366, 465, 623, 1039
BciVI GTATCC 1 cut(s) 1619
BcnI CCSGG 1 cut(s) 1445
BcoDI GTCTC 3 cut(s) 749, 995, 1523
BcuI ACTAGT 1 cut(s) 1018
BfmI CTRYAG 2 cut(s) 795, 1363
BfuI GTATCC 1 cut(s) 1619
BisI GCNGC 7 cut(s) 58, 276, 439, 538, 1119, 1391, 1699
BlsI GCNGC 7 cut(s) 59, 277, 440, 539, 1120, 1392, 1700
BmcAI AGTACT 1 cut(s) 1740
Bme1390I CCNGG 5 cut(s) 366, 465, 623, 1039, 1445
Bme18I GGWCC 2 cut(s) 639, 1315
BmeT110I CYCGRG 1 cut(s) 1693
BmgT120I GGNCC 3 cut(s) 639, 1315, 1696
BmiI GGNNCC 1 cut(s) 1317
BmrFI CCNGG 5 cut(s) 366, 465, 623, 1039, 1445
BmrI ACTGGG 2 cut(s) 655, 995
BmsI GCATC 3 cut(s) 262, 1553, 1653
BmuI ACTGGG 2 cut(s) 655, 995
BplI GAGNNNNNCTC 2 cut(s) 26, 58
Bpu10I CCTNAGC 1 cut(s) 19
BpuEI CTTGAG 3 cut(s) 939, 1547, 1588
BpuMI CCSGG 1 cut(s) 1445
BsaHI GRCGYC 1 cut(s) 1359
BsaJI CCNNGG 5 cut(s) 739, 823, 1038, 1467, 1648
BsaXI ACNNNNNCTCC 6 cut(s) 16, 46, 735, 765, 1136, 1166
Bsc4I CCNNNNNNNGG 3 cut(s) 51, 394, 395
Bse118I RCCGGY 1 cut(s) 225
Bse1I ACTGG 7 cut(s) 178, 523, 529, 535, 650, 990, 1736
Bse3DI GCAATG 1 cut(s) 1617
BseBI CCWGG 4 cut(s) 366, 465, 623, 1039
BseDI CCNNGG 5 cut(s) 739, 823, 1038, 1467, 1648
BseGI GGATG 2 cut(s) 265, 1644
BseLI CCNNNNNNNGG 3 cut(s) 51, 394, 395
BseMI GCAATG 1 cut(s) 1617
BseMII CTCAG 1 cut(s) 691
BseNI ACTGG 7 cut(s) 178, 523, 529, 535, 650, 990, 1736
BseRI GAGGAG 1 cut(s) 1144
BseXI GCAGC 6 cut(s) 44, 287, 450, 549, 1130, 1377
Bsh1236I CGCG 2 cut(s) 731, 1252
BshFI GGCC 4 cut(s) 177, 1426, 1591, 1698
BsiHKAI GWGCWC 2 cut(s) 213, 1138
BsiHKCI CYCGRG 1 cut(s) 1693
BsiSI CCGG 5 cut(s) 45, 226, 342, 1131, 1445
BslFI GGGAC 2 cut(s) 149, 1312
BslI CCNNNNNNNGG 3 cut(s) 51, 394, 395
BsmAI GTCTC 3 cut(s) 749, 995, 1523
BsmFI GGGAC 2 cut(s) 149, 1312
BsmI GAATGC 2 cut(s) 27, 570
BsnI GGCC 4 cut(s) 177, 1426, 1591, 1698
BsoBI CYCGRG 1 cut(s) 1693
Bsp1286I GDGCHC 2 cut(s) 213, 1138
Bsp143I GATC 2 cut(s) 1113, 1267
Bsp68I TCGCGA 1 cut(s) 1252
BspACI CCGC 2 cut(s) 729, 1699
BspANI GGCC 4 cut(s) 177, 1426, 1591, 1698
BspCNI CTCAG 1 cut(s) 690
BspFNI CGCG 2 cut(s) 731, 1252
BspHI TCATGA 1 cut(s) 403
BspLI GGNNCC 1 cut(s) 1317
BsrDI GCAATG 1 cut(s) 1617
BsrFI RCCGGY 1 cut(s) 225
BsrI ACTGG 7 cut(s) 178, 523, 529, 535, 650, 990, 1736
BssAI RCCGGY 1 cut(s) 225
BssECI CCNNGG 5 cut(s) 739, 823, 1038, 1467, 1648
BssMI GATC 2 cut(s) 1113, 1267
BssNI GRCGYC 1 cut(s) 1359
BssT1I CCWWGG 1 cut(s) 739
Bst2UI CCWGG 4 cut(s) 366, 465, 623, 1039
Bst4CI ACNGT 8 cut(s) 97, 434, 604, 815, 842, 847, 1367, 1413
Bst6I CTCTTC 1 cut(s) 1428
BstACI GRCGYC 1 cut(s) 1359
BstAPI GCANNNNNTGC 1 cut(s) 54
BstC8I GCNNGC 4 cut(s) 227, 301, 362, 578
BstDEI CTNAG 2 cut(s) 19, 677
BstDSI CCRYGG 2 cut(s) 1467, 1648
BstF5I GGATG 2 cut(s) 265, 1644
BstFNI CGCG 2 cut(s) 731, 1252
BstHHI GCGC 1 cut(s) 443
BstKTI GATC 2 cut(s) 1116, 1270
BstMAI GTCTC 3 cut(s) 749, 995, 1523
BstMBI GATC 2 cut(s) 1113, 1267
BstMWI GCNNNNNNNGC 7 cut(s) 24, 54, 834, 1127, 1300, 1553, 1698
BstNI CCWGG 4 cut(s) 366, 465, 623, 1039
BstNSI RCATGY 2 cut(s) 303, 712
BstSCI CCNGG 5 cut(s) 364, 463, 621, 1037, 1443
BstSFI CTRYAG 2 cut(s) 795, 1363
BstUI CGCG 2 cut(s) 731, 1252
BstV1I GCAGC 6 cut(s) 44, 287, 450, 549, 1130, 1377
BsuI GTATCC 1 cut(s) 1619
BsuRI GGCC 4 cut(s) 177, 1426, 1591, 1698
BtgI CCRYGG 2 cut(s) 1467, 1648
BtsCI GGATG 2 cut(s) 265, 1644
BtsIMutI CAGTG 4 cut(s) 362, 1409, 1532, 1679
BtuMI TCGCGA 1 cut(s) 1252
Cac8I GCNNGC 4 cut(s) 227, 301, 362, 578
CaiI CAGNNNCTG 1 cut(s) 1635
CciI TCATGA 1 cut(s) 403
CfoI GCGC 1 cut(s) 443
Cfr10I RCCGGY 1 cut(s) 225
Cfr13I GGNCC 3 cut(s) 639, 1315, 1696
CseI GACGC 1 cut(s) 1309
Csp6I GTAC 6 cut(s) 93, 385, 843, 1144, 1309, 1739
CspCI CAANNNNNGTGG 2 cut(s) 1386, 1421
CviAII CATG 7 cut(s) 147, 300, 404, 636, 709, 1165, 1597
CviQI GTAC 6 cut(s) 93, 385, 843, 1144, 1309, 1739
DdeI CTNAG 2 cut(s) 19, 677
DpnI GATC 2 cut(s) 1115, 1269
DpnII GATC 2 cut(s) 1113, 1267
DrdI GACNNNNNNGTC 3 cut(s) 732, 818, 1508
DseDI GACNNNNNNGTC 3 cut(s) 732, 818, 1508
Eam1104I CTCTTC 1 cut(s) 1428
EarI CTCTTC 1 cut(s) 1428
Ecl136II GAGCTC 1 cut(s) 211
Eco130I CCWWGG 1 cut(s) 739
Eco147I AGGCCT 2 cut(s) 1426, 1591
Eco24I GRGCYC 1 cut(s) 213
Eco32I GATATC 1 cut(s) 409
Eco47I GGWCC 2 cut(s) 639, 1315
Eco53kI GAGCTC 1 cut(s) 211
Eco57I CTGAAG 2 cut(s) 1130, 1449
Eco88I CYCGRG 1 cut(s) 1693
EcoICRI GAGCTC 1 cut(s) 211
EcoRII CCWGG 4 cut(s) 364, 463, 621, 1037
EcoRV GATATC 1 cut(s) 409
EcoT14I CCWWGG 1 cut(s) 739
EcoT22I ATGCAT 1 cut(s) 301
EcoT38I GRGCYC 1 cut(s) 213
ErhI CCWWGG 1 cut(s) 739
FaeI CATG 7 cut(s) 150, 303, 407, 639, 712, 1168, 1600
FaqI GGGAC 2 cut(s) 149, 1312
FatI CATG 7 cut(s) 146, 299, 403, 635, 708, 1164, 1596
FauI CCCGC 1 cut(s) 736
FblI GTMKAC 1 cut(s) 1362
Fnu4HI GCNGC 7 cut(s) 58, 276, 439, 538, 1119, 1391, 1699
FokI GGATG 2 cut(s) 272, 1631
FriOI GRGCYC 1 cut(s) 213
Fsp4HI GCNGC 7 cut(s) 58, 276, 439, 538, 1119, 1391, 1699
GlaI GCGC 1 cut(s) 442
GluI GCNGC 7 cut(s) 58, 276, 439, 538, 1119, 1391, 1699
HaeIII GGCC 4 cut(s) 177, 1426, 1591, 1698
HapII CCGG 5 cut(s) 45, 226, 342, 1131, 1445
HgaI GACGC 1 cut(s) 1309
HhaI GCGC 1 cut(s) 443
Hin1I GRCGYC 1 cut(s) 1359
Hin1II CATG 7 cut(s) 150, 303, 407, 639, 712, 1168, 1600
Hin6I GCGC 1 cut(s) 441
HinP1I GCGC 1 cut(s) 441
HincII GTYRAC 1 cut(s) 961
HindII GTYRAC 1 cut(s) 961
HinfI GANTC 7 cut(s) 35, 733, 819, 1082, 1258, 1486, 1632
HpaII CCGG 5 cut(s) 45, 226, 342, 1131, 1445
HphI GGTGA 5 cut(s) 988, 1027, 1504, 1585, 1735
Hpy166II GTNNAC 5 cut(s) 183, 850, 961, 1024, 1363
Hpy188I TCNGA 6 cut(s) 234, 680, 905, 1237, 1244, 1373
Hpy188III TCNNGA 7 cut(s) 32, 404, 1251, 1265, 1496, 1526, 1672
Hpy8I GTNNAC 5 cut(s) 183, 850, 961, 1024, 1363
Hpy99I CGWCG 3 cut(s) 416, 509, 1090
HpyAV CCTTC 7 cut(s) 16, 167, 169, 368, 824, 1616, 1712
HpyCH4III ACNGT 8 cut(s) 97, 434, 604, 815, 842, 847, 1367, 1413
HpyCH4IV ACGT 2 cut(s) 1068, 1359
HpyCH4V TGCA 8 cut(s) 275, 299, 303, 438, 537, 1150, 1294, 1556
HpyF10VI GCNNNNNNNGC 7 cut(s) 24, 54, 834, 1127, 1300, 1553, 1698
HpyF3I CTNAG 2 cut(s) 19, 677
HpySE526I ACGT 2 cut(s) 1068, 1359
Hsp92I GRCGYC 1 cut(s) 1359
Hsp92II CATG 7 cut(s) 150, 303, 407, 639, 712, 1168, 1600
HspAI GCGC 1 cut(s) 441
KroI GCCGGC 1 cut(s) 225
KroNI GCCGGC 1 cut(s) 227
Kzo9I GATC 2 cut(s) 1113, 1267
LmnI GCTCC 8 cut(s) 24, 47, 446, 905, 1133, 1244, 1387, 1615
Lsp1109I GCAGC 6 cut(s) 44, 287, 450, 549, 1130, 1377
LweI GCATC 3 cut(s) 262, 1553, 1653
MaeII ACGT 2 cut(s) 1068, 1359
MaeIII GTNAC 6 cut(s) 368, 598, 714, 1101, 1355, 1510
MalI GATC 2 cut(s) 1115, 1269
MboI GATC 2 cut(s) 1113, 1267
MboII GAAGA 9 cut(s) 182, 275, 667, 791, 1052, 1123, 1442, 1445, 1447
MfeI CAATTG 2 cut(s) 244, 252
MhlI GDGCHC 2 cut(s) 213, 1138
MluCI AATT 6 cut(s) 244, 252, 457, 469, 1216, 1491
MlyI GAGTC 5 cut(s) 44, 742, 828, 1480, 1626
MmeI TCCRAC 1 cut(s) 1215
Mph1103I ATGCAT 1 cut(s) 301
MroNI GCCGGC 1 cut(s) 225
MroXI GAANNNNTTC 1 cut(s) 472
MseI TTAA 1 cut(s) 141
MslI CAYNNNNRTG 3 cut(s) 969, 1163, 1182
MspA1I CMGCKG 2 cut(s) 1121, 1342
MspI CCGG 5 cut(s) 45, 226, 342, 1131, 1445
MspR9I CCNGG 5 cut(s) 366, 465, 623, 1039, 1445
MunI CAATTG 2 cut(s) 244, 252
Mva1269I GAATGC 2 cut(s) 27, 570
MvaI CCWGG 4 cut(s) 366, 465, 623, 1039
MvnI CGCG 2 cut(s) 731, 1252
MwoI GCNNNNNNNGC 7 cut(s) 24, 54, 834, 1127, 1300, 1553, 1698
NaeI GCCGGC 1 cut(s) 227
NciI CCSGG 1 cut(s) 1445
NdeII GATC 2 cut(s) 1113, 1267
NgoMIV GCCGGC 1 cut(s) 225
NlaIII CATG 7 cut(s) 150, 303, 407, 639, 712, 1168, 1600
NlaIV GGNNCC 1 cut(s) 1317
NmeAIII GCCGAG 1 cut(s) 804
NmuCI GTSAC 3 cut(s) 598, 1355, 1510
NruI TCGCGA 1 cut(s) 1252
NsiI ATGCAT 1 cut(s) 301
NspI RCATGY 2 cut(s) 303, 712
PaeI GCATGC 1 cut(s) 303
PagI TCATGA 1 cut(s) 403
PceI AGGCCT 2 cut(s) 1426, 1591
PcsI WCGNNNNNNNCGW 2 cut(s) 1091, 1464
PctI GAATGC 2 cut(s) 27, 570
PdiI GCCGGC 1 cut(s) 227
PdmI GAANNNNTTC 1 cut(s) 472
PfeI GAWTC 2 cut(s) 1082, 1258
PflFI GACNNNGTC 1 cut(s) 1508
PkrI GCNGC 7 cut(s) 59, 277, 440, 539, 1120, 1392, 1700
PleI GAGTC 5 cut(s) 43, 741, 827, 1480, 1626
PpsI GAGTC 5 cut(s) 43, 741, 827, 1480, 1626
PsiI TTATAA 1 cut(s) 932
Psp124BI GAGCTC 1 cut(s) 213
Psp6I CCWGG 4 cut(s) 364, 463, 621, 1037
PspGI CCWGG 4 cut(s) 364, 463, 621, 1037
PspN4I GGNNCC 1 cut(s) 1317
PspPI GGNCC 3 cut(s) 639, 1315, 1696
PstNI CAGNNNCTG 1 cut(s) 1635
PsyI GACNNNGTC 1 cut(s) 1508
PvuII CAGCTG 2 cut(s) 1121, 1342
RruI TCGCGA 1 cut(s) 1252
RsaI GTAC 6 cut(s) 94, 386, 844, 1145, 1310, 1740
RsaNI GTAC 6 cut(s) 93, 385, 843, 1144, 1309, 1739
RseI CAYNNNNRTG 3 cut(s) 969, 1163, 1182
SacI GAGCTC 1 cut(s) 213
SaqAI TTAA 1 cut(s) 141
SatI GCNGC 7 cut(s) 58, 276, 439, 538, 1119, 1391, 1699
Sau3AI GATC 2 cut(s) 1113, 1267
Sau96I GGNCC 3 cut(s) 639, 1315, 1696
ScaI AGTACT 1 cut(s) 1740
SchI GAGTC 5 cut(s) 44, 742, 828, 1480, 1626
ScrFI CCNGG 5 cut(s) 366, 465, 623, 1039, 1445
SduI GDGCHC 2 cut(s) 213, 1138
SfaNI GCATC 3 cut(s) 262, 1553, 1653
SfcI CTRYAG 2 cut(s) 795, 1363
SinI GGWCC 2 cut(s) 639, 1315
SmiMI CAYNNNNRTG 3 cut(s) 969, 1163, 1182
SmlI CTYRAG 3 cut(s) 954, 1526, 1567
SmoI CTYRAG 3 cut(s) 954, 1526, 1567
SpeI ACTAGT 1 cut(s) 1018
SphI GCATGC 1 cut(s) 303
Sse9I AATT 6 cut(s) 244, 252, 457, 469, 1216, 1491
SseBI AGGCCT 2 cut(s) 1426, 1591
SsiI CCGC 2 cut(s) 729, 1699
SstI GAGCTC 1 cut(s) 213
StuI AGGCCT 2 cut(s) 1426, 1591
StyD4I CCNGG 5 cut(s) 364, 463, 621, 1037, 1443
StyI CCWWGG 1 cut(s) 739
TaaI ACNGT 8 cut(s) 97, 434, 604, 815, 842, 847, 1367, 1413
TaiI ACGT 2 cut(s) 1071, 1362
TaqI TCGA 4 cut(s) 213, 411, 896, 1497
TasI AATT 6 cut(s) 244, 252, 457, 469, 1216, 1491
TatI WGTACW 1 cut(s) 1738
TauI GCSGC 1 cut(s) 1701
TfiI GAWTC 2 cut(s) 1082, 1258
Tru1I TTAA 1 cut(s) 141
Tru9I TTAA 1 cut(s) 141
TscAI CASTG 4 cut(s) 362, 1416, 1539, 1686
TseFI GTSAC 3 cut(s) 598, 1355, 1510
TseI GCWGC 6 cut(s) 57, 275, 438, 537, 1118, 1390
Tsp45I GTSAC 3 cut(s) 598, 1355, 1510
TspGWI ACGGA 4 cut(s) 372, 522, 1456, 1665
TspRI CASTG 4 cut(s) 362, 1416, 1539, 1686
Tth111I GACNNNGTC 1 cut(s) 1508
VpaK11BI GGWCC 2 cut(s) 639, 1315
XapI RAATTY 2 cut(s) 457, 1491
XbaI TCTAGA 1 cut(s) 1264
XceI RCATGY 2 cut(s) 303, 712
XmiI GTMKAC 1 cut(s) 1362
XmnI GAANNNNTTC 1 cut(s) 472
ZraI GACGTC 1 cut(s) 1360
ZrmI AGTACT 1 cut(s) 1740
Zsp2I ATGCAT 1 cut(s) 301
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.