Rw5G012300

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000066
Physical Location & Seq
Reverse (-)
14406444 .. 14409430
2987 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G012300.1

Sequence Viewer

Length: 2355 bp
ATGGTCTTATACCGGACAATGGTCATTTCGCAGCTGATCTCATTGGTAGTGGTGGCCATAGCTGCAGTACCAATAGCCGACGAAGCCCTCCCGCCTCTTCAAGCCCTGCCTGGATGCCTTGACCACTGTGGCAATCTCACAGTCCCCTATCCATTCGGCATAGGCGACGGTTGTTACCTTATAAAAAAATTCAACCTCACCTGTGACGAATCCAGCCAACCTCCAACACTGTACTTGGGGGATGGACCGGATGGTATCACTATTACTAACATATCCGTGGCCAAACGTGAGTTGCAAATTTTGTTTTACATAGCCTATGATTGCTACGACCAACAGGGTAATCAAACGGAATCCAATCTCCCATGGCTCTGGGTCGCCCCTCCGTTCACCATCTCTGGTACCAGAAACAAGTTCATTGCCGTTGGCTATGACACCTCTGCCATTTTTATGGGGTACCGCCCTTACCCTAATGATGATGTTGGGTACGTAGCCGGGTGCATCATAAGTGACATTCCTGACAATACTACTGCTGCTGCTGACGAGTCATGCACCGGCAATGGGTGTTGCCAAACTTCAATGCCTGCAGACGGACTTAAAAATCAAACTGTCAAGTTGAGTAGTTACTCTAGCCATACCAATGTACGGAATTTCAACCGATGCAGCTACGCTTTCATTGTGGAAGATGGCAATTTCACATTTAACCCGACTACAAGTTTTCAACAACTGAACAACACTGAACGGCTTCCTATGATTCTTAATTGGGAAATCGCGGACGTTTCATGTGCTGAAGCTGAAAAGAGGCAGGATTATGCTTGCAAGGCGCATAGCAAGTGTGTCAATACCATCGACATCAAGACTGAACCGTCTGGTTATTATTGCGAGTGCTGGCCAGGCTACCAAGGCAACCCATATCTCCCAGATGGCTGTCAAGATATTAATGAATGCATCGCCTCAACGGACCCCTGCAACAATGGAACGTGCAAAAATTCAGATGGAGATTACACATGTACATGTAAGAAAGGTTTCAGAAACGAAAACCCAAGGAGTTGCGTACAGAACACTCCTATATCAAAGAACAAGTCCCTGAAAGTTTCCTTGGGTATTGATTGGTTGAATTCGTGCGTAGGTGTCTCCCTGAGCTTCTTAGGGGTATTGCTTGTAACATTTTGGATATACTGCGGACTGAAGAGAAGAAAGTTCAAGATACTCAAAGAGAAGTACTTCAAAGACAATGGTGGCTTCTTGTTACAACAAAAATTGGAGAATTTCGATGGACCTCAAGCAGCCAAAATATTTACTAGAGAAGAACTTAAGAAGGCCACAAACAACTTTCATGACAGTTTAAAAATTGGTGAAGGAGGCTATGGATTTGTTTACAAAGGAACACTATCAAATGGAAATGAGGTTGCCATAAAGGTGTCAAAAAGTAGTGCCCCAATGACCCAAAGTAATCAGTTCATTAATGAGGTGATTGTTCTTTCTCAGATTAACCACAGAAATGTGGTGAAGCTTCTAGGTTGTTGTTTAGAGACCCAAACGCCTTTATTAGTGTATGAGTTCGTCAGCAACGGCACACTTTATGAGCATATTCATAAAAAGAATGGCAAGGGACCGCTTTCCTTTGGGCTACGAATGAAGATAGCAACAGAAACAGCAGGATCACTAGCATACTTGCACTACTCTACTTCGATGCAAATTGTGCACCGTGATGTGAAGGCAGCAAACATTCTACTGGATGAGAATTTCACAGCGAAAGTGTCAGACTTTGGAGCTTCAAAATTGGTTCCCGAAGATCAAAATCAACTGTCTACTTTAGTGCAAGGGACGATAGGGTACTTGGACCCTGAATATCTTCAGTCAAACACACTGACAGAGAAAAGTGACGTATATAGCTTCGGCGTTGTCCTTGTGGAGCTAATAACAAGTCAAGTGGCAATTTCTTATCAGAAGCCTGAGGCGGAGAGAAGCTTAGCAAAATTTTTTGTTACTTCTGTGGAAGAGAATCGCCTAGATCAGATCCTTGATCATGAAATAATCAAAGAGGGGAGCTTCGAAACAGCTGAACAAGTAGCCCATCTGGCCAAAAGTTGTTTAAGTTTGAAAGGGAGCGATAGGCCTACCATGAAAGAAGTAGCAATGAAGCTAGAGGGAGTATTGCAAGTTATGGCAAAGCATCCATGGGGAAAGGCTGATGCCTCACCAAAAGAGACTGATTACTTGCAGCTTGCGTCACATTCAAAAGCCTACGTTGTTGATGTTAGAGGTGATGAAGGTGAAGTTATAACCAGTATTGACTACGACCGGAGCATCCAGATTCAACCCCTGATGATGAAGCCTTATGATGGAGGCCGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK cEGF

Protein Analysis

784

Amino Acids

86.74

Weight (kDa)

5.55

Isoelectric Point (pI)

32.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 39 - 100 6.8e-11 Wall-associated receptor kinase galacturonan-binding
EGF_CA PF07645 311 - 350 3.9e-07 Calcium-binding EGF domain
Pkinase PF00069 445 - 712 3.6e-48 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 449 - 715 3.1e-50 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21210 AT1G21230 AT1G21240 AT1G21240 AT1G21245 AT1G21250 AT1G21270 AT1G22720 AT3G25490
fragaria_vesca FvH4_2g35051 FvH4_2g35051 FvH4_2g35060 FvH4_3g10281 FvH4_3g10285 FvH4_3g10286 FvH4_3g10311 FvH4_3g10311 FvH4_3g10320 FvH4_3g10332 FvH4_3g10340 FvH4_3g10350 FvH4_3g10360 FvH4_3g10390 FvH4_3g10412 FvH4_3g10413 FvH4_3g10461 FvH4_3g10462 FvH4_3g10470 FvH4_3g10510 FvH4_3g10520 FvH4_3g10530 FvH4_3g10550 FvH4_3g10560 FvH4_3g10611 FvH4_3g11730 FvH4_6g53140 FvH4_6g53142 FvH4_7g31880 FvH4_7g31910 FvH4_7g31920
malus_domestica MD05G1269900.v1.1 MD05G1270000.v1.1 MD05G1270200.v1.1 MD05G1270400.v1.1 MD05G1270500.v1.1 MD05G1270600.v1.1 MD05G1270800.v1.1 MD05G1270900.v1.1 MD07G1183800.v1.1 MD08G1107600.v1.1 MD10G1248500.v1.1 MD10G1249200.v1.1 MD10G1249500.v1.1 MD10G1250000.v1.1 MD10G1250500.v1.1 MD10G1250900.v1.1 MD10G1251200.v1.1 MD10G1251400.v1.1 MD13G1004600.v1.1 MD15G1088400.v1.1
prunus_persica Prupe.1G442400_v2.0.a1 Prupe.1G442500_v2.0.a1 Prupe.2G223400_v2.0.a1 Prupe.4G091900_v2.0.a1 Prupe.4G093200_v2.0.a1 Prupe.4G093300_v2.0.a1 Prupe.4G093400_v2.0.a1 Prupe.4G093500_v2.0.a1 Prupe.4G094100_v2.0.a1 Prupe.4G094200_v2.0.a1 Prupe.4G094300_v2.0.a1 Prupe.6G358300_v2.0.a1
pyrus_communis pycom08g08990 pycom10g20660 pycom10g20670 pycom10g20680 pycom10g20710 pycom10g20740 pycom13g00400 pycom15g08290
rosa_chinensis RchiOBHm_Chr1g0326861 RchiOBHm_Chr1g0326871 RchiOBHm_Chr1g0331391 RchiOBHm_Chr1g0336911 RchiOBHm_Chr1g0364001 RchiOBHm_Chr2g0174541 RchiOBHm_Chr2g0175041 RchiOBHm_Chr2g0175101 RchiOBHm_Chr2g0175121 RchiOBHm_Chr3g0486331 RchiOBHm_Chr5g0015581 RchiOBHm_Chr5g0016551 RchiOBHm_Chr5g0016561 RchiOBHm_Chr5g0016581 RchiOBHm_Chr5g0016601 RchiOBHm_Chr5g0016611 RchiOBHm_Chr5g0016641 RchiOBHm_Chr5g0016661 RchiOBHm_Chr5g0016691 RchiOBHm_Chr5g0016701 RchiOBHm_Chr5g0016731 RchiOBHm_Chr5g0016741 RchiOBHm_Chr5g0016811 RchiOBHm_Chr5g0016841 RchiOBHm_Chr5g0016871 RchiOBHm_Chr5g0016881 RchiOBHm_Chr5g0016931 RchiOBHm_Chr5g0017021 RchiOBHm_Chr5g0017041 RchiOBHm_Chr5g0017071 RchiOBHm_Chr5g0017091 RchiOBHm_Chr5g0017121 RchiOBHm_Chr5g0017141 RchiOBHm_Chr5g0017291 RchiOBHm_Chr5g0017321 RchiOBHm_Chr5g0017401 RchiOBHm_Chr5g0019001 RchiOBHm_Chr5g0019071 RchiOBHm_Chr5g0028941 RchiOBHm_Chr5g0052141 RchiOBHm_Chr5g0054721 RchiOBHm_Chr5g0054801 RchiOBHm_Chr5g0054931 RchiOBHm_Chr5g0054941 RchiOBHm_Chr6g0259291 RchiOBHm_Chr6g0292991 RchiOBHm_Chr6g0306911
rosa_laevigata RLG00000009465 RLG00000009468 RLG00000010755 RLG00000011976 RLG00000014541 RLG00000014542 RLG00000015169 RLG00000022308 RLG00000027529 RLG00000029324 RLG00000030244 RLG00000030245 RLG00000032210 RLG00000032211 RLG00000032257 RLG00000032259 RLG00000032260 RLG00000032261 RLG00000032262 RLG00000032268 RLG00000032269 RLG00000032272 RLG00000032282 RLG00000032283 RLG00000032308 RLG00000032413 RLG00000033129 RLG00000034945
rosa_multiflora Rmu_co8241929.1_g000001 Rmu_co8269227.1_g000001 Rmu_co8461259.1_g000001 Rmu_co8495385.1_g000001 Rmu_sc0000028.1_g000003 Rmu_sc0000083.1_g000009 Rmu_sc0000083.1_g000010 Rmu_sc0000083.1_g000031 Rmu_sc0000083.1_g000043 Rmu_sc0000083.1_g000046 Rmu_sc0000083.1_g000047 Rmu_sc0000563.1_g000024 Rmu_sc0000842.1_g000012 Rmu_sc0001235.1_g000001 Rmu_sc0001235.1_g000005 Rmu_sc0001235.1_g000009 Rmu_sc0001235.1_g000015 Rmu_sc0001235.1_g000016 Rmu_sc0001235.1_g000017 Rmu_sc0001473.1_g000036 Rmu_sc0001607.1_g000016 Rmu_sc0001788.1_g000006 Rmu_sc0002018.1_g000008 Rmu_sc0002028.1_g000001 Rmu_sc0002680.1_g000011 Rmu_sc0002798.1_g000023 Rmu_sc0002886.1_g000023 Rmu_sc0002989.1_g000012 Rmu_sc0003064.1_g000009 Rmu_sc0003382.1_g000006 Rmu_sc0003630.1_g000002 Rmu_sc0003630.1_g000047 Rmu_sc0003630.1_g000066 Rmu_sc0003630.1_g000067 Rmu_sc0003649.1_g000010 Rmu_sc0003688.1_g000005 Rmu_sc0003920.1_g000007 Rmu_sc0004765.1_g000007 Rmu_sc0004765.1_g000008 Rmu_sc0004920.1_g000011 Rmu_sc0005223.1_g000008 Rmu_sc0005223.1_g000010 Rmu_sc0005223.1_g000015 Rmu_sc0005223.1_g000016 Rmu_sc0005319.1_g000014 Rmu_sc0005319.1_g000015 Rmu_sc0005319.1_g000018 Rmu_sc0005319.1_g000019 Rmu_sc0005319.1_g000025 Rmu_sc0005418.1_g000007 Rmu_sc0005418.1_g000012 Rmu_sc0006106.1_g000011 Rmu_sc0006914.1_g000012 Rmu_sc0007023.1_g000001 Rmu_sc0007662.1_g000001 Rmu_sc0007920.1_g000002 Rmu_sc0007920.1_g000013 Rmu_sc0008053.1_g000016 Rmu_sc0008053.1_g000017 Rmu_sc0008053.1_g000018 Rmu_sc0008053.1_g000023 Rmu_sc0008053.1_g000050 Rmu_sc0009042.1_g000007 Rmu_sc0009042.1_g000008 Rmu_sc0010642.1_g000008 Rmu_sc0011109.1_g000002 Rmu_sc0011314.1_g000006 Rmu_sc0011974.1_g000006 Rmu_sc0012179.1_g000009 Rmu_sc0015169.1_g000001 Rmu_sc0016368.1_g000002 Rmu_sc0022659.1_g000001 Rmu_sc0027371.1_g000001 Rmu_sc0028213.1_g000001 Rmu_sc0034526.1_g000001 Rmu_sc0039366.1_g000002 Rmu_ssc0000050.1_g000084 Rmu_ssc0000050.1_g000088 Rmu_ssc0000167.1_g000017
rosa_roxburghii Rroxscaffold_1G00011340 Rroxscaffold_1G00025710 Rroxscaffold_1G00025870 Rroxscaffold_1G00026070 Rroxscaffold_1G00026170 Rroxscaffold_1G00026200 Rroxscaffold_1G00026270 Rroxscaffold_1G00050950 Rroxscaffold_1G00058900 Rroxscaffold_1G00060040 Rroxscaffold_1G00060060 Rroxscaffold_1G00060070 Rroxscaffold_1G00060080 Rroxscaffold_1G00060110 Rroxscaffold_1G00060120 Rroxscaffold_1G00060140 Rroxscaffold_1G00060170 Rroxscaffold_1G00060210 Rroxscaffold_1G00060220 Rroxscaffold_1G00060240 Rroxscaffold_1G00060250 Rroxscaffold_1G00060260 Rroxscaffold_1G00060300 Rroxscaffold_1G00060320 Rroxscaffold_1G00060330 Rroxscaffold_1G00060900 Rroxscaffold_1G00060920 Rroxscaffold_1G00073980 Rroxscaffold_2G00077560 Rroxscaffold_2G00077580 Rroxscaffold_2G00146990 Rroxscaffold_3G00236200 Rroxscaffold_3G00236210 Rroxscaffold_3G00236220 Rroxscaffold_4G00292760 Rroxscaffold_4G00315200 Rroxscaffold_4G00315240 Rroxscaffold_4G00326240 Rroxscaffold_4G00328980 Rroxscaffold_5G00341890 Rroxscaffold_7G00161350 Rroxscaffold_7G00174490 Rroxscaffold_7G00206550
rosa_rugosa Rorug01G0093000 Rorug01G0093100 Rorug01G0128800 Rorug01G0312000 Rorug01G0312000 Rorug02G0581100 Rorug03G0224600 Rorug05G0028500 Rorug05G0032700 Rorug05G0032800 Rorug05G0032900 Rorug05G0032900 Rorug05G0033000 Rorug05G0033100 Rorug05G0033300 Rorug05G0033400 Rorug05G0033900 Rorug05G0034900 Rorug05G0035000 Rorug05G0046900 Rorug05G0047500 Rorug05G0047500 Rorug05G0111600 Rorug05G0287900 Rorug05G0288700 Rorug05G0586900 Rorug06G0231700 Rorug06G0354300 Rorug06G0354400
rosa_samantha Rh1DG122200 Rh2DG686800 Rh5BG136600 Rh6AG344500 Rh6BG351900 Rh6CG359000 Rh6CG359100 Rh6CG387600 Rh6CG480500 Rh6DG344600 Rh6DG467400
rosa_wichuraiana Rw0G010250 Rw0G014820 Rw0G021400 Rw0G023100 Rw1G009450 Rw1G012100 Rw1G012180 Rw1G028360 Rw2G054260 Rw3G024370 Rw3G024420 Rw3G024470 Rw5G010850 Rw5G011010 Rw5G011020 Rw5G011050 Rw5G011080 Rw5G011120 Rw5G012300 Rw5G018470 Rw5G033830 Rw6G008930 Rw6G030070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 182, 2282
AasI GACNNNNNNGTC 1 cut(s) 862
Acc65I GGTACC 2 cut(s) 398, 453
AccB1I GGYRCC 2 cut(s) 398, 453
AccI GTMKAC 1 cut(s) 1808
AccII CGCG 1 cut(s) 770
AciI CCGC 6 cut(s) 92, 457, 770, 1179, 1613, 1958
AclWI GGATC 2 cut(s) 1666, 2011
AcoI YGGCCR 4 cut(s) 54, 279, 887, 2079
AcsI RAATTY 8 cut(s) 188, 297, 646, 985, 1114, 1264, 1741, 1976
AcuI CTGAAG 3 cut(s) 807, 1205, 1838
AfiI CCNNNNNNNGG 5 cut(s) 19, 558, 587, 642, 2342
AflII CTTAAG 1 cut(s) 1310
AflIII ACRYGT 2 cut(s) 1004, 1010
AjnI CCWGG 2 cut(s) 109, 889
AjuI GAANNNNNNNTTGG 2 cut(s) 1079, 1111
Alw21I GWGCWC 1 cut(s) 1704
Alw26I GTCTC 3 cut(s) 1135, 1523, 2201
Alw44I GTGCAC 1 cut(s) 1700
AlwI GGATC 2 cut(s) 1666, 2011
AoxI GGCC 7 cut(s) 54, 279, 887, 1317, 2079, 2114, 2347
ApaLI GTGCAC 1 cut(s) 1700
ApeKI GCWGC 8 cut(s) 31, 62, 530, 533, 660, 1283, 1718, 2221
ApoI RAATTY 8 cut(s) 188, 297, 646, 985, 1114, 1264, 1741, 1976
ArsI GACNNNNNNTTYG 2 cut(s) 1064, 1096
AseI ATTAAT 2 cut(s) 936, 1461
Asp700I GAANNNNTTC 4 cut(s) 741, 1022, 1220, 1851
Asp718I GGTACC 2 cut(s) 398, 453
AspLEI GCGC 1 cut(s) 823
AspS9I GGNCC 5 cut(s) 245, 958, 1274, 1610, 1840
AsuC2I CCSGG 1 cut(s) 493
AsuHPI GGTGA 8 cut(s) 190, 379, 1364, 1480, 1516, 2190, 2276, 2285
AsuII TTCGAA 1 cut(s) 2052
AvaII GGWCC 5 cut(s) 245, 958, 1274, 1610, 1840
AxyI CCTNAGG 1 cut(s) 1953
BaeGI GKGCMC 2 cut(s) 1435, 1704
BalI TGGCCA 4 cut(s) 56, 281, 889, 2081
BanI GGYRCC 2 cut(s) 398, 453
Bbv12I GWGCWC 1 cut(s) 1704
BbvI GCAGC 8 cut(s) 43, 49, 517, 520, 672, 1295, 1730, 2233
BceAI ACGGC 3 cut(s) 404, 755, 1585
BciT130I CCWGG 2 cut(s) 111, 891
BclI TGATCA 1 cut(s) 2023
BcnI CCSGG 1 cut(s) 493
BcoDI GTCTC 3 cut(s) 1135, 1523, 2201
BfaI CTAG 6 cut(s) 627, 1299, 1514, 1664, 2009, 2144
BfmI CTRYAG 2 cut(s) 63, 582
BfrI CTTAAG 1 cut(s) 1310
BglI GCCNNNNNGGC 1 cut(s) 2078
BisI GCNGC 8 cut(s) 32, 63, 531, 534, 661, 1284, 1719, 2222
BlpI GCTNAGC 1 cut(s) 1969
BlsI GCNGC 8 cut(s) 33, 64, 532, 535, 662, 1285, 1720, 2223
BmcAI AGTACT 1 cut(s) 1220
Bme1390I CCNGG 3 cut(s) 111, 493, 891
Bme18I GGWCC 5 cut(s) 245, 958, 1274, 1610, 1840
BmgT120I GGNCC 5 cut(s) 245, 958, 1274, 1610, 1840
BmiI GGNNCC 6 cut(s) 400, 455, 960, 1611, 1785, 1842
BmrFI CCNGG 3 cut(s) 111, 493, 891
BmsI GCATC 8 cut(s) 104, 507, 647, 954, 1680, 2182, 2182, 2316
BoxI GACNNNNGTC 1 cut(s) 20
Bpu10I CCTNAGC 1 cut(s) 1136
Bpu1102I GCTNAGC 1 cut(s) 1969
Bpu14I TTCGAA 1 cut(s) 2052
BpuEI CTTGAG 1 cut(s) 1263
BpuMI CCSGG 1 cut(s) 493
BsaAI YACGTR 1 cut(s) 487
BsaBI GATNNNNATC 1 cut(s) 1797
BsaI GGTCTC 1 cut(s) 1523
BsaJI CCNNGG 6 cut(s) 276, 362, 898, 1040, 1095, 2177
BsaWI WCCGGW 3 cut(s) 12, 247, 2301
Bsc4I CCNNNNNNNGG 5 cut(s) 19, 558, 587, 642, 2342
Bse118I RCCGGY 1 cut(s) 551
Bse1I ACTGG 2 cut(s) 1737, 2286
Bse21I CCTNAGG 1 cut(s) 1953
Bse3DI GCAATG 3 cut(s) 414, 562, 2142
Bse8I GATNNNNATC 1 cut(s) 1797
BseBI CCWGG 2 cut(s) 111, 891
BseDI CCNNGG 6 cut(s) 276, 362, 898, 1040, 1095, 2177
BseGI GGATG 6 cut(s) 119, 247, 256, 1741, 2173, 2307
BseJI GATNNNNATC 1 cut(s) 1797
BseLI CCNNNNNNNGG 5 cut(s) 19, 558, 587, 642, 2342
BseMI GCAATG 3 cut(s) 414, 562, 2142
BseMII CTCAG 3 cut(s) 1127, 1496, 1944
BseNI ACTGG 2 cut(s) 1737, 2286
BseSI GKGCMC 2 cut(s) 1435, 1704
BseXI GCAGC 8 cut(s) 43, 49, 517, 520, 672, 1295, 1730, 2233
Bsh1236I CGCG 1 cut(s) 770
Bsh1285I CGRYCG 1 cut(s) 2302
BshFI GGCC 7 cut(s) 56, 281, 889, 1319, 2081, 2116, 2349
BshNI GGYRCC 2 cut(s) 398, 453
BsiEI CGRYCG 1 cut(s) 2302
BsiHKAI GWGCWC 1 cut(s) 1704
BsiSI CCGG 5 cut(s) 13, 248, 492, 552, 2302
BslFI GGGAC 4 cut(s) 128, 1066, 1623, 1837
BslI CCNNNNNNNGG 5 cut(s) 19, 558, 587, 642, 2342
BsmAI GTCTC 3 cut(s) 1135, 1523, 2201
BsmFI GGGAC 4 cut(s) 128, 1066, 1623, 1837
BsmI GAATGC 1 cut(s) 947
BsnI GGCC 7 cut(s) 56, 281, 889, 1319, 2081, 2116, 2349
Bso31I GGTCTC 1 cut(s) 1523
Bsp119I TTCGAA 1 cut(s) 2052
Bsp1286I GDGCHC 2 cut(s) 1435, 1704
Bsp1407I TGTACA 1 cut(s) 1007
Bsp143I GATC 6 cut(s) 36, 1658, 1792, 2011, 2016, 2023
Bsp1720I GCTNAGC 1 cut(s) 1969
Bsp19I CCATGG 2 cut(s) 362, 2177
BspACI CCGC 6 cut(s) 92, 457, 770, 1179, 1613, 1958
BspANI GGCC 7 cut(s) 56, 281, 889, 1319, 2081, 2116, 2349
BspCNI CTCAG 3 cut(s) 1128, 1495, 1945
BspFNI CGCG 1 cut(s) 770
BspHI TCATGA 2 cut(s) 1333, 2026
BspLI GGNNCC 6 cut(s) 400, 455, 960, 1611, 1785, 1842
BspMAI CTGCAG 2 cut(s) 67, 586
BspPI GGATC 2 cut(s) 1666, 2011
BspT104I TTCGAA 1 cut(s) 2052
BspT107I GGYRCC 2 cut(s) 398, 453
BspTI CTTAAG 1 cut(s) 1310
BspTNI GGTCTC 1 cut(s) 1523
BsrDI GCAATG 3 cut(s) 414, 562, 2142
BsrFI RCCGGY 1 cut(s) 551
BsrGI TGTACA 1 cut(s) 1007
BsrI ACTGG 2 cut(s) 1737, 2286
BssAI RCCGGY 1 cut(s) 551
BssECI CCNNGG 6 cut(s) 276, 362, 898, 1040, 1095, 2177
BssMI GATC 6 cut(s) 36, 1658, 1792, 2011, 2016, 2023
BssT1I CCWWGG 5 cut(s) 362, 898, 1040, 1095, 2177
Bst2UI CCWGG 2 cut(s) 111, 891
Bst4CI ACNGT 9 cut(s) 128, 142, 170, 231, 607, 864, 1340, 1706, 1806
Bst6I CTCTTC 3 cut(s) 102, 1181, 1992
BstAFI CTTAAG 1 cut(s) 1310
BstAPI GCANNNNNTGC 1 cut(s) 1699
BstAUI TGTACA 1 cut(s) 1007
BstBAI YACGTR 1 cut(s) 487
BstBI TTCGAA 1 cut(s) 2052
BstC8I GCNNGC 4 cut(s) 582, 814, 887, 2226
BstDEI CTNAG 5 cut(s) 1136, 1144, 1482, 1953, 1969
BstDSI CCRYGG 3 cut(s) 276, 362, 2177
BstF5I GGATG 6 cut(s) 119, 247, 256, 1741, 2173, 2307
BstFNI CGCG 1 cut(s) 770
BstHHI GCGC 1 cut(s) 823
BstKTI GATC 6 cut(s) 39, 1661, 1795, 2014, 2019, 2026
BstMAI GTCTC 3 cut(s) 1135, 1523, 2201
BstMBI GATC 6 cut(s) 36, 1658, 1792, 2011, 2016, 2023
BstMCI CGRYCG 1 cut(s) 2302
BstMWI GCNNNNNNNGC 7 cut(s) 62, 83, 818, 891, 900, 1699, 2078
BstNI CCWGG 2 cut(s) 111, 891
BstNSI RCATGY 2 cut(s) 1008, 1014
BstPAI GACNNNNGTC 1 cut(s) 20
BstSCI CCNGG 3 cut(s) 109, 491, 889
BstSFI CTRYAG 2 cut(s) 63, 582
BstSLI GKGCMC 2 cut(s) 1435, 1704
BstSNI TACGTA 1 cut(s) 487
BstUI CGCG 1 cut(s) 770
BstV1I GCAGC 8 cut(s) 43, 49, 517, 520, 672, 1295, 1730, 2233
BstX2I RGATCY 1 cut(s) 2016
BstXI CCANNNNNNTGG 2 cut(s) 369, 448
BstYI RGATCY 1 cut(s) 2016
Bsu36I CCTNAGG 1 cut(s) 1953
BsuRI GGCC 7 cut(s) 56, 281, 889, 1319, 2081, 2116, 2349
BtgI CCRYGG 3 cut(s) 276, 362, 2177
BtgZI GCGATG 1 cut(s) 931
BtsCI GGATG 6 cut(s) 119, 247, 256, 1741, 2173, 2307
BtsIMutI CAGTG 4 cut(s) 124, 227, 732, 1865
Cac8I GCNNGC 4 cut(s) 582, 814, 887, 2226
CciI TCATGA 2 cut(s) 1333, 2026
CfoI GCGC 1 cut(s) 823
Cfr10I RCCGGY 1 cut(s) 551
Cfr13I GGNCC 5 cut(s) 245, 958, 1274, 1610, 1840
CseI GACGC 1 cut(s) 2217
CspCI CAANNNNNGTGG 2 cut(s) 1911, 1946
CviAII CATG 9 cut(s) 363, 546, 780, 1005, 1011, 1334, 2027, 2122, 2178
DdeI CTNAG 5 cut(s) 1136, 1144, 1482, 1953, 1969
DpnI GATC 6 cut(s) 38, 1660, 1794, 2013, 2018, 2025
DpnII GATC 6 cut(s) 36, 1658, 1792, 2011, 2016, 2023
DraI TTTAAA 1 cut(s) 1344
DrdI GACNNNNNNGTC 1 cut(s) 862
DseDI GACNNNNNNGTC 1 cut(s) 862
EaeI YGGCCR 4 cut(s) 54, 279, 887, 2079
Eam1104I CTCTTC 3 cut(s) 102, 1181, 1992
EarI CTCTTC 3 cut(s) 102, 1181, 1992
EciI GGCGGA 1 cut(s) 1973
Eco105I TACGTA 1 cut(s) 487
Eco130I CCWWGG 5 cut(s) 362, 898, 1040, 1095, 2177
Eco147I AGGCCT 1 cut(s) 2116
Eco31I GGTCTC 1 cut(s) 1523
Eco47I GGWCC 5 cut(s) 245, 958, 1274, 1610, 1840
Eco57I CTGAAG 3 cut(s) 807, 1205, 1838
Eco81I CCTNAGG 1 cut(s) 1953
EcoRI GAATTC 1 cut(s) 1114
EcoRII CCWGG 2 cut(s) 109, 889
EcoT14I CCWWGG 5 cut(s) 362, 898, 1040, 1095, 2177
EcoT22I ATGCAT 1 cut(s) 947
ErhI CCWWGG 5 cut(s) 362, 898, 1040, 1095, 2177
FaeI CATG 9 cut(s) 366, 549, 783, 1008, 1014, 1337, 2030, 2125, 2181
FaqI GGGAC 4 cut(s) 128, 1066, 1623, 1837
FatI CATG 9 cut(s) 362, 545, 779, 1004, 1010, 1333, 2026, 2121, 2177
FauI CCCGC 1 cut(s) 99
FbaI TGATCA 1 cut(s) 2023
FblI GTMKAC 1 cut(s) 1808
Fnu4HI GCNGC 8 cut(s) 32, 63, 531, 534, 661, 1284, 1719, 2222
FokI GGATG 6 cut(s) 126, 254, 263, 1748, 2160, 2294
Fsp4HI GCNGC 8 cut(s) 32, 63, 531, 534, 661, 1284, 1719, 2222
FspBI CTAG 6 cut(s) 627, 1299, 1514, 1664, 2009, 2144
GlaI GCGC 1 cut(s) 822
GluI GCNGC 8 cut(s) 32, 63, 531, 534, 661, 1284, 1719, 2222
HaeIII GGCC 7 cut(s) 56, 281, 889, 1319, 2081, 2116, 2349
HapII CCGG 5 cut(s) 13, 248, 492, 552, 2302
HgaI GACGC 1 cut(s) 2217
HhaI GCGC 1 cut(s) 823
Hin1II CATG 9 cut(s) 366, 549, 783, 1008, 1014, 1337, 2030, 2125, 2181
Hin6I GCGC 1 cut(s) 821
HinP1I GCGC 1 cut(s) 821
HindIII AAGCTT 2 cut(s) 1508, 1966
HinfI GANTC 6 cut(s) 209, 350, 542, 751, 2002, 2314
HpaII CCGG 5 cut(s) 13, 248, 492, 552, 2302
HphI GGTGA 8 cut(s) 190, 379, 1364, 1480, 1516, 2190, 2276, 2285
Hpy166II GTNNAC 4 cut(s) 387, 1375, 1702, 1809
Hpy188I TCNGA 6 cut(s) 991, 1028, 1485, 1762, 1947, 2016
Hpy188III TCNNGA 8 cut(s) 515, 853, 929, 1201, 1334, 1787, 2027, 2311
Hpy8I GTNNAC 4 cut(s) 387, 1375, 1702, 1809
Hpy99I CGWCG 2 cut(s) 83, 170
HpyAV CCTTC 4 cut(s) 1309, 1349, 1708, 2264
HpyCH4III ACNGT 9 cut(s) 128, 142, 170, 231, 607, 864, 1340, 1706, 1806
HpyCH4IV ACGT 6 cut(s) 286, 486, 774, 977, 1884, 2247
HpyF10VI GCNNNNNNNGC 7 cut(s) 62, 83, 818, 891, 900, 1699, 2078
HpyF3I CTNAG 5 cut(s) 1136, 1144, 1482, 1953, 1969
HpySE526I ACGT 6 cut(s) 286, 486, 774, 977, 1884, 2247
Hsp92II CATG 9 cut(s) 366, 549, 783, 1008, 1014, 1337, 2030, 2125, 2181
HspAI GCGC 1 cut(s) 821
KpnI GGTACC 2 cut(s) 402, 457
Ksp22I TGATCA 1 cut(s) 2023
Kzo9I GATC 6 cut(s) 36, 1658, 1792, 2011, 2016, 2023
LmnI GCTCC 5 cut(s) 1769, 1912, 2046, 2106, 2304
Lsp1109I GCAGC 8 cut(s) 43, 49, 517, 520, 672, 1295, 1730, 2233
LweI GCATC 8 cut(s) 104, 507, 647, 954, 1680, 2182, 2182, 2316
MaeI CTAG 6 cut(s) 627, 1299, 1514, 1664, 2009, 2144
MaeII ACGT 6 cut(s) 286, 486, 774, 977, 1884, 2247
MaeIII GTNAC 9 cut(s) 173, 203, 506, 620, 1159, 1245, 1880, 1984, 2229
MalI GATC 6 cut(s) 38, 1660, 1794, 2013, 2018, 2025
MboI GATC 6 cut(s) 36, 1658, 1792, 2011, 2016, 2023
MboII GAAGA 9 cut(s) 89, 692, 1198, 1203, 1316, 1648, 1802, 1844, 2009
MflI RGATCY 1 cut(s) 2016
MhlI GDGCHC 2 cut(s) 1435, 1704
MlsI TGGCCA 4 cut(s) 56, 281, 889, 2081
MluNI TGGCCA 4 cut(s) 56, 281, 889, 2081
MlyI GAGTC 1 cut(s) 551
MmeI TCCRAC 1 cut(s) 248
Mox20I TGGCCA 4 cut(s) 56, 281, 889, 2081
Mph1103I ATGCAT 1 cut(s) 947
MroXI GAANNNNTTC 4 cut(s) 741, 1022, 1220, 1851
MscI TGGCCA 4 cut(s) 56, 281, 889, 2081
MseI TTAA 9 cut(s) 594, 699, 756, 936, 1311, 1343, 1461, 1488, 2093
MslI CAYNNNNRTG 7 cut(s) 275, 446, 636, 1009, 1415, 1497, 1707
Msp20I TGGCCA 4 cut(s) 56, 281, 889, 2081
MspA1I CMGCKG 2 cut(s) 34, 2060
MspCI CTTAAG 1 cut(s) 1310
MspI CCGG 5 cut(s) 13, 248, 492, 552, 2302
MspR9I CCNGG 3 cut(s) 111, 493, 891
Mva1269I GAATGC 1 cut(s) 947
MvaI CCWGG 2 cut(s) 111, 891
MvnI CGCG 1 cut(s) 770
MwoI GCNNNNNNNGC 7 cut(s) 62, 83, 818, 891, 900, 1699, 2078
NciI CCSGG 1 cut(s) 493
NcoI CCATGG 2 cut(s) 362, 2177
NdeII GATC 6 cut(s) 36, 1658, 1792, 2011, 2016, 2023
NlaIII CATG 9 cut(s) 366, 549, 783, 1008, 1014, 1337, 2030, 2125, 2181
NlaIV GGNNCC 6 cut(s) 400, 455, 960, 1611, 1785, 1842
NmuCI GTSAC 4 cut(s) 203, 506, 1880, 2229
NsiI ATGCAT 1 cut(s) 947
NspI RCATGY 2 cut(s) 1008, 1014
NspV TTCGAA 1 cut(s) 2052
PagI TCATGA 2 cut(s) 1333, 2026
PceI AGGCCT 1 cut(s) 2116
PciI ACATGT 2 cut(s) 1004, 1010
PcsI WCGNNNNNNNCGW 1 cut(s) 162
PctI GAATGC 1 cut(s) 947
PdmI GAANNNNTTC 4 cut(s) 741, 1022, 1220, 1851
PfeI GAWTC 5 cut(s) 209, 350, 751, 2002, 2314
PkrI GCNGC 8 cut(s) 33, 64, 532, 535, 662, 1285, 1720, 2223
PleI GAGTC 1 cut(s) 550
PpsI GAGTC 1 cut(s) 550
Ppu21I YACGTR 1 cut(s) 487
PscI ACATGT 2 cut(s) 1004, 1010
PshAI GACNNNNGTC 1 cut(s) 20
PshBI ATTAAT 2 cut(s) 936, 1461
PsiI TTATAA 2 cut(s) 182, 2282
Psp6I CCWGG 2 cut(s) 109, 889
PspGI CCWGG 2 cut(s) 109, 889
PspN4I GGNNCC 6 cut(s) 400, 455, 960, 1611, 1785, 1842
PspPI GGNCC 5 cut(s) 245, 958, 1274, 1610, 1840
PstI CTGCAG 2 cut(s) 67, 586
PsuI RGATCY 1 cut(s) 2016
PvuII CAGCTG 2 cut(s) 34, 2060
RseI CAYNNNNRTG 7 cut(s) 275, 446, 636, 1009, 1415, 1497, 1707
SaqAI TTAA 9 cut(s) 594, 699, 756, 936, 1311, 1343, 1461, 1488, 2093
SatI GCNGC 8 cut(s) 32, 63, 531, 534, 661, 1284, 1719, 2222
Sau3AI GATC 6 cut(s) 36, 1658, 1792, 2011, 2016, 2023
Sau96I GGNCC 5 cut(s) 245, 958, 1274, 1610, 1840
ScaI AGTACT 1 cut(s) 1220
SchI GAGTC 1 cut(s) 551
ScrFI CCNGG 3 cut(s) 111, 493, 891
SduI GDGCHC 2 cut(s) 1435, 1704
SfaNI GCATC 8 cut(s) 104, 507, 647, 954, 1680, 2182, 2182, 2316
SfcI CTRYAG 2 cut(s) 63, 582
SfuI TTCGAA 1 cut(s) 2052
SinI GGWCC 5 cut(s) 245, 958, 1274, 1610, 1840
SmiMI CAYNNNNRTG 7 cut(s) 275, 446, 636, 1009, 1415, 1497, 1707
SmlI CTYRAG 2 cut(s) 1278, 1310
SmoI CTYRAG 2 cut(s) 1278, 1310
SnaBI TACGTA 1 cut(s) 487
SseBI AGGCCT 1 cut(s) 2116
SsiI CCGC 6 cut(s) 92, 457, 770, 1179, 1613, 1958
SspI AATATT 1 cut(s) 1293
SspMI CTAG 6 cut(s) 627, 1299, 1514, 1664, 2009, 2144
StuI AGGCCT 1 cut(s) 2116
StyD4I CCNGG 3 cut(s) 109, 491, 889
StyI CCWWGG 5 cut(s) 362, 898, 1040, 1095, 2177
TaaI ACNGT 9 cut(s) 128, 142, 170, 231, 607, 864, 1340, 1706, 1806
TaiI ACGT 6 cut(s) 289, 489, 777, 980, 1887, 2250
TaqI TCGA 4 cut(s) 846, 1269, 1688, 2052
TatI WGTACW 3 cut(s) 231, 1007, 1218
TfiI GAWTC 5 cut(s) 209, 350, 751, 2002, 2314
Tru1I TTAA 9 cut(s) 594, 699, 756, 936, 1311, 1343, 1461, 1488, 2093
Tru9I TTAA 9 cut(s) 594, 699, 756, 936, 1311, 1343, 1461, 1488, 2093
TscAI CASTG 4 cut(s) 131, 234, 739, 1872
TseFI GTSAC 4 cut(s) 203, 506, 1880, 2229
TseI GCWGC 8 cut(s) 31, 62, 530, 533, 660, 1283, 1718, 2221
Tsp45I GTSAC 4 cut(s) 203, 506, 1880, 2229
TspGWI ACGGA 6 cut(s) 265, 362, 372, 603, 658, 971
TspRI CASTG 4 cut(s) 131, 234, 739, 1872
Vha464I CTTAAG 1 cut(s) 1310
VneI GTGCAC 1 cut(s) 1700
VpaK11BI GGWCC 5 cut(s) 245, 958, 1274, 1610, 1840
VspI ATTAAT 2 cut(s) 936, 1461
XapI RAATTY 8 cut(s) 188, 297, 646, 985, 1114, 1264, 1741, 1976
XceI RCATGY 2 cut(s) 1008, 1014
XmiI GTMKAC 1 cut(s) 1808
XmnI GAANNNNTTC 4 cut(s) 741, 1022, 1220, 1851
XspI CTAG 6 cut(s) 627, 1299, 1514, 1664, 2009, 2144
ZrmI AGTACT 1 cut(s) 1220
Zsp2I ATGCAT 1 cut(s) 947
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.