MD05G1269900.v1.1

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000066
Physical Location & Seq
Forward (+)
40487125 .. 40489679
2555 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1269900.v1.1.491

Sequence Viewer

Length: 2265 bp
ATGGCCATAAATGGGAGGACCCTGCTTATTCAACTCTCTTTGGCCGTAATATCAGCTGCTACAATTACCAAATTAGCGGCAGCTGATGGACCCCTGACTCAAGCCCTGCCTGGCTGCAAAGACAAATGTGGTGATCTCACAATTCCATATCCATTTGGCATAGGTGACGGTTGTTACCTCCGACCGGAATTCAACATCACTTGTGACCACTCCGCCACACCCCCAGCAGCAAACTTAACCGGTCAGACATTCCGTATTACCAAATTTTCCCTTGGTGAGGGTGAGTTGCAAGTAATGATGTTCATATCCCGAGATTGCTATGCCATGGTTGACTCCCATGCTCGCAGAACCTACCGGAACAACCCAGCACTCTGGTTGCCTCCTCCTTATACCATCTCTGATACCAAGAACCGATTCATCGCAGTTGGTTGTGACACTTACGCCCTTTTCCAAGGTTATAGAGGCGAAGAAAGGTTCATCACTGGGTGCATGTCCTTATGCAACAGCCTTGGAAGCGTCAGTGACTCTAATTGCGCAGGCGTTGGATGTTGCCAAACTTCCATCCCCAGCGGACTGAAAAATTGTACGGTTCAATTGAGTAGCTATTACAATCATAGCTTTATAATGGACTTCAATCCCTGCAGCTATGCTTTCACTGTCGAACAAGGCCAGTTCGAATTCAGCAGCACAAGTTTTGATCAACTCACCAACACAAGCCTACTTCCGATGGTTATTAATTGGGAAATTGAAGATAAGACTTGTTATTCTGCTTGCAAGGCAAATAATAGCGTGTGTGTCAACCGGGCCGTTAATATCAGTGGTTCTGGTTACATTTGTCAGTGCTTGGGAGGTTACCAAGGGAATCCATACCTCCCCCATAGTTGCCAAGATATTGATGAGTGCAAGTCCTCAAACCCGTGCAGTATCGGAACGTGCGTAAATGTACCAGGAAACTTTTCCTGTAAATGTCCCGAAGGGTACAAAAATGATGGCACGGATAGACAGAGTTGCATCAAGGACAACCCGAACAATCTCCTGCTTATGATTTCATTAGGTGTCACTGGTGGGTTCTTGATTTTACTAGCTGGAATTTCATGGACGTATTGGGCAATGCAGAGAAGAAAGATCATCAAACTCCAAGAAAAATACTTCAAGGAAAATGGCGGCTTGTTGTTGCAACAACAACTGGCTAGTCAAGAAGGCTCCGTGGAGGCAATAAAAATCTTTACTGGAGAAGAACTTAAGAGGGCCACAAACAATTACCATGACAGTAGAATCGTTGGCGAAGGAGGCTATGGAATAGTTTACAAAGGTGTATTGCCAGATAGCAATAAAGTAGTTGCCATAAAGAAGTCCAAAATTGGTGTGCCAACCCAAAAGGAGCAGTTTGTTAATGAGTTGATTGTTCTATCTCAAATCAACCATAGAAATGTGGTGAGACTGTTAGGTTGCTGTTTAGAGACATCAGTGCCATTACTAGTTTACGAGTTCATCACGAATGGCACTCTTTTTGAGCACATCCATCATAAAAATGGCAGAGGATCATCATTTTCATGGGAATTACGATTGAAGATAGCAGCAGAAACCGCAGGAGCACTAGCATACTTACACTCCTCCGCTCTCATGCAAATCATACACCGAGATGTGAAAGCGACAAATATACTATTAGATGACAATTACACAGCAAAAGTGTCAGACTTCGGAGCTTCACGATTGATTCCCTTGGATCAAAATCAGCTAACAACATTAGTGCAAGGGACATATGGATACTTAGACCCTGAATACTTCTTAACGAATCAACTAACCGAAAAGAGTGATGTGTATAGCTTTGGGGTTGTCCTTATGGAGCTACTAACCAGCAAAGTAGCACTTTCTTTTGCCAGGCCTGAGGAAGAGAGAAACCTAGCAAGCTTGTTTGTTTGTGCAATGGAGGAGGATCGCTTGAATCAAATTCTTGATCAAGACATAGTCAACGATGGAAACATTCAGACAGTGAAACATGTGGCCAATCTCGCAAAAGGATGTGTGAGGTTAAAAGGAGGGGACAGGCCTACGATGAAAGAAGTAGCGATGGAGTTAGAGGGAATGAGAACTACATCAAATCATTCATGGGGGAAAGCTGATTTCACTCCAGAAGAGACTCAGTACTATCTTGGTTCACCTAGTTCAGACACTTACAGTACTGTTGCTGTTAGAGGTGACTTTAGTGGAACGAAGGGCAGCATGGAAATCCAAATGTTAATGCCATATGATGATGGCAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK hEGF

Protein Analysis

755

Amino Acids

83.07

Weight (kDa)

5.81

Isoelectric Point (pI)

29.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 39 - 98 2.2e-11 Wall-associated receptor kinase galacturonan-binding
EGF_CA PF07645 297 - 337 1e-06 Calcium-binding EGF domain
EGF PF00008 301 - 329 5.9e-06 EGF-like domain
Pkinase PF00069 421 - 689 2.2e-46 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 425 - 693 7.2e-45 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21210 AT1G21230 AT1G21240 AT1G21240 AT1G21245 AT1G21250 AT1G21270 AT1G22720 AT3G25490
fragaria_vesca FvH4_2g35051 FvH4_2g35051 FvH4_2g35060 FvH4_3g10281 FvH4_3g10285 FvH4_3g10286 FvH4_3g10311 FvH4_3g10311 FvH4_3g10320 FvH4_3g10332 FvH4_3g10340 FvH4_3g10350 FvH4_3g10360 FvH4_3g10390 FvH4_3g10412 FvH4_3g10413 FvH4_3g10461 FvH4_3g10462 FvH4_3g10470 FvH4_3g10510 FvH4_3g10520 FvH4_3g10530 FvH4_3g10550 FvH4_3g10560 FvH4_3g10611 FvH4_3g11730 FvH4_6g53140 FvH4_6g53142 FvH4_7g31880 FvH4_7g31910 FvH4_7g31920
malus_domestica MD05G1269900.v1.1 MD05G1270000.v1.1 MD05G1270200.v1.1 MD05G1270400.v1.1 MD05G1270500.v1.1 MD05G1270600.v1.1 MD05G1270800.v1.1 MD05G1270900.v1.1 MD07G1183800.v1.1 MD08G1107600.v1.1 MD10G1248500.v1.1 MD10G1249200.v1.1 MD10G1249500.v1.1 MD10G1250000.v1.1 MD10G1250500.v1.1 MD10G1250900.v1.1 MD10G1251200.v1.1 MD10G1251400.v1.1 MD13G1004600.v1.1 MD15G1088400.v1.1
prunus_persica Prupe.1G442400_v2.0.a1 Prupe.1G442500_v2.0.a1 Prupe.2G223400_v2.0.a1 Prupe.4G091900_v2.0.a1 Prupe.4G093200_v2.0.a1 Prupe.4G093300_v2.0.a1 Prupe.4G093400_v2.0.a1 Prupe.4G093500_v2.0.a1 Prupe.4G094100_v2.0.a1 Prupe.4G094200_v2.0.a1 Prupe.4G094300_v2.0.a1 Prupe.6G358300_v2.0.a1
pyrus_communis pycom08g08990 pycom10g20660 pycom10g20670 pycom10g20680 pycom10g20710 pycom10g20740 pycom13g00400 pycom15g08290
rosa_chinensis RchiOBHm_Chr1g0326861 RchiOBHm_Chr1g0326871 RchiOBHm_Chr1g0331391 RchiOBHm_Chr1g0336911 RchiOBHm_Chr1g0364001 RchiOBHm_Chr2g0174541 RchiOBHm_Chr2g0175041 RchiOBHm_Chr2g0175101 RchiOBHm_Chr2g0175121 RchiOBHm_Chr3g0486331 RchiOBHm_Chr5g0015581 RchiOBHm_Chr5g0016551 RchiOBHm_Chr5g0016561 RchiOBHm_Chr5g0016581 RchiOBHm_Chr5g0016601 RchiOBHm_Chr5g0016611 RchiOBHm_Chr5g0016641 RchiOBHm_Chr5g0016661 RchiOBHm_Chr5g0016691 RchiOBHm_Chr5g0016701 RchiOBHm_Chr5g0016731 RchiOBHm_Chr5g0016741 RchiOBHm_Chr5g0016811 RchiOBHm_Chr5g0016841 RchiOBHm_Chr5g0016871 RchiOBHm_Chr5g0016881 RchiOBHm_Chr5g0016931 RchiOBHm_Chr5g0017021 RchiOBHm_Chr5g0017041 RchiOBHm_Chr5g0017071 RchiOBHm_Chr5g0017091 RchiOBHm_Chr5g0017121 RchiOBHm_Chr5g0017141 RchiOBHm_Chr5g0017291 RchiOBHm_Chr5g0017321 RchiOBHm_Chr5g0017401 RchiOBHm_Chr5g0019001 RchiOBHm_Chr5g0019071 RchiOBHm_Chr5g0028941 RchiOBHm_Chr5g0052141 RchiOBHm_Chr5g0054721 RchiOBHm_Chr5g0054801 RchiOBHm_Chr5g0054931 RchiOBHm_Chr5g0054941 RchiOBHm_Chr6g0259291 RchiOBHm_Chr6g0292991 RchiOBHm_Chr6g0306911
rosa_laevigata RLG00000009465 RLG00000009468 RLG00000010755 RLG00000011976 RLG00000014541 RLG00000014542 RLG00000015169 RLG00000022308 RLG00000027529 RLG00000029324 RLG00000030244 RLG00000030245 RLG00000032210 RLG00000032211 RLG00000032257 RLG00000032259 RLG00000032260 RLG00000032261 RLG00000032262 RLG00000032268 RLG00000032269 RLG00000032272 RLG00000032282 RLG00000032283 RLG00000032308 RLG00000032413 RLG00000033129 RLG00000034945
rosa_multiflora Rmu_co8241929.1_g000001 Rmu_co8269227.1_g000001 Rmu_co8461259.1_g000001 Rmu_co8495385.1_g000001 Rmu_sc0000028.1_g000003 Rmu_sc0000083.1_g000009 Rmu_sc0000083.1_g000010 Rmu_sc0000083.1_g000031 Rmu_sc0000083.1_g000043 Rmu_sc0000083.1_g000046 Rmu_sc0000083.1_g000047 Rmu_sc0000563.1_g000024 Rmu_sc0000842.1_g000012 Rmu_sc0001235.1_g000001 Rmu_sc0001235.1_g000005 Rmu_sc0001235.1_g000009 Rmu_sc0001235.1_g000015 Rmu_sc0001235.1_g000016 Rmu_sc0001235.1_g000017 Rmu_sc0001473.1_g000036 Rmu_sc0001607.1_g000016 Rmu_sc0001788.1_g000006 Rmu_sc0002018.1_g000008 Rmu_sc0002028.1_g000001 Rmu_sc0002680.1_g000011 Rmu_sc0002798.1_g000023 Rmu_sc0002886.1_g000023 Rmu_sc0002989.1_g000012 Rmu_sc0003064.1_g000009 Rmu_sc0003382.1_g000006 Rmu_sc0003630.1_g000002 Rmu_sc0003630.1_g000047 Rmu_sc0003630.1_g000066 Rmu_sc0003630.1_g000067 Rmu_sc0003649.1_g000010 Rmu_sc0003688.1_g000005 Rmu_sc0003920.1_g000007 Rmu_sc0004765.1_g000007 Rmu_sc0004765.1_g000008 Rmu_sc0004920.1_g000011 Rmu_sc0005223.1_g000008 Rmu_sc0005223.1_g000010 Rmu_sc0005223.1_g000015 Rmu_sc0005223.1_g000016 Rmu_sc0005319.1_g000014 Rmu_sc0005319.1_g000015 Rmu_sc0005319.1_g000018 Rmu_sc0005319.1_g000019 Rmu_sc0005319.1_g000025 Rmu_sc0005418.1_g000007 Rmu_sc0005418.1_g000012 Rmu_sc0006106.1_g000011 Rmu_sc0006914.1_g000012 Rmu_sc0007023.1_g000001 Rmu_sc0007662.1_g000001 Rmu_sc0007920.1_g000002 Rmu_sc0007920.1_g000013 Rmu_sc0008053.1_g000016 Rmu_sc0008053.1_g000017 Rmu_sc0008053.1_g000018 Rmu_sc0008053.1_g000023 Rmu_sc0008053.1_g000050 Rmu_sc0009042.1_g000007 Rmu_sc0009042.1_g000008 Rmu_sc0010642.1_g000008 Rmu_sc0011109.1_g000002 Rmu_sc0011314.1_g000006 Rmu_sc0011974.1_g000006 Rmu_sc0012179.1_g000009 Rmu_sc0015169.1_g000001 Rmu_sc0016368.1_g000002 Rmu_sc0022659.1_g000001 Rmu_sc0027371.1_g000001 Rmu_sc0028213.1_g000001 Rmu_sc0034526.1_g000001 Rmu_sc0039366.1_g000002 Rmu_ssc0000050.1_g000084 Rmu_ssc0000050.1_g000088 Rmu_ssc0000167.1_g000017
rosa_roxburghii Rroxscaffold_1G00011340 Rroxscaffold_1G00025710 Rroxscaffold_1G00025870 Rroxscaffold_1G00026070 Rroxscaffold_1G00026170 Rroxscaffold_1G00026200 Rroxscaffold_1G00026270 Rroxscaffold_1G00050950 Rroxscaffold_1G00058900 Rroxscaffold_1G00060040 Rroxscaffold_1G00060060 Rroxscaffold_1G00060070 Rroxscaffold_1G00060080 Rroxscaffold_1G00060110 Rroxscaffold_1G00060120 Rroxscaffold_1G00060140 Rroxscaffold_1G00060170 Rroxscaffold_1G00060210 Rroxscaffold_1G00060220 Rroxscaffold_1G00060240 Rroxscaffold_1G00060250 Rroxscaffold_1G00060260 Rroxscaffold_1G00060300 Rroxscaffold_1G00060320 Rroxscaffold_1G00060330 Rroxscaffold_1G00060900 Rroxscaffold_1G00060920 Rroxscaffold_1G00073980 Rroxscaffold_2G00077560 Rroxscaffold_2G00077580 Rroxscaffold_2G00146990 Rroxscaffold_3G00236200 Rroxscaffold_3G00236210 Rroxscaffold_3G00236220 Rroxscaffold_4G00292760 Rroxscaffold_4G00315200 Rroxscaffold_4G00315240 Rroxscaffold_4G00326240 Rroxscaffold_4G00328980 Rroxscaffold_5G00341890 Rroxscaffold_7G00161350 Rroxscaffold_7G00174490 Rroxscaffold_7G00206550
rosa_rugosa Rorug01G0093000 Rorug01G0093100 Rorug01G0128800 Rorug01G0312000 Rorug01G0312000 Rorug02G0581100 Rorug03G0224600 Rorug05G0028500 Rorug05G0032700 Rorug05G0032800 Rorug05G0032900 Rorug05G0032900 Rorug05G0033000 Rorug05G0033100 Rorug05G0033300 Rorug05G0033400 Rorug05G0033900 Rorug05G0034900 Rorug05G0035000 Rorug05G0046900 Rorug05G0047500 Rorug05G0047500 Rorug05G0111600 Rorug05G0287900 Rorug05G0288700 Rorug05G0586900 Rorug06G0231700 Rorug06G0354300 Rorug06G0354400
rosa_samantha Rh1DG122200 Rh2DG686800 Rh5BG136600 Rh6AG344500 Rh6BG351900 Rh6CG359000 Rh6CG359100 Rh6CG387600 Rh6CG480500 Rh6DG344600 Rh6DG467400
rosa_wichuraiana Rw0G010250 Rw0G014820 Rw0G021400 Rw0G023100 Rw1G009450 Rw1G012100 Rw1G012180 Rw1G028360 Rw2G054260 Rw3G024370 Rw3G024420 Rw3G024470 Rw5G010850 Rw5G011010 Rw5G011020 Rw5G011050 Rw5G011080 Rw5G011120 Rw5G012300 Rw5G018470 Rw5G033830 Rw6G008930 Rw6G030070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 623
Acc16I TGCGCA 1 cut(s) 535
AccBSI CCGCTC 1 cut(s) 1619
AciI CCGC 6 cut(s) 77, 213, 570, 1164, 1587, 1617
AclWI GGATC 3 cut(s) 1549, 1734, 1944
AcoI YGGCCR 3 cut(s) 3, 42, 2004
AcsI RAATTY 5 cut(s) 188, 263, 677, 1089, 1950
AdeI CACNNNGTG 1 cut(s) 486
AfaI GTAC 5 cut(s) 586, 945, 980, 2147, 2182
AfiI CCNNNNNNNGG 3 cut(s) 12, 184, 277
AflII CTTAAG 1 cut(s) 1241
AflIII ACRYGT 1 cut(s) 1999
AgeI ACCGGT 1 cut(s) 239
AgsI TTSAA 8 cut(s) 32, 193, 593, 634, 749, 1153, 1570, 1945
AhlI ACTAGT 1 cut(s) 1477
AjnI CCWGG 3 cut(s) 109, 946, 1880
AjuI GAANNNNNNNTTGG 2 cut(s) 398, 430
Alw21I GWGCWC 2 cut(s) 1518, 1597
Alw26I GTCTC 3 cut(s) 1432, 1454, 2132
AlwI GGATC 3 cut(s) 1549, 1734, 1944
Ama87I CYCGRG 1 cut(s) 309
AoxI GGCC 8 cut(s) 3, 42, 667, 804, 1248, 1883, 2004, 2048
ApeKI GCWGC 8 cut(s) 56, 80, 114, 227, 642, 684, 1577, 2220
ApoI RAATTY 5 cut(s) 188, 263, 677, 1089, 1950
AseI ATTAAT 1 cut(s) 735
AsiGI ACCGGT 1 cut(s) 239
Asp700I GAANNNNTTC 2 cut(s) 413, 955
AspLEI GCGC 1 cut(s) 536
AspS9I GGNCC 4 cut(s) 18, 89, 804, 1248
AsuC2I CCSGG 1 cut(s) 803
AsuHPI GGTGA 8 cut(s) 143, 176, 287, 293, 697, 1447, 2151, 2210
AsuII TTCGAA 1 cut(s) 675
AvaI CYCGRG 1 cut(s) 309
AvaII GGWCC 2 cut(s) 18, 89
AxyI CCTNAGG 1 cut(s) 1887
BalI TGGCCA 2 cut(s) 5, 2006
Bbv12I GWGCWC 2 cut(s) 1518, 1597
BbvI GCAGC 8 cut(s) 43, 92, 101, 239, 654, 696, 1589, 2232
BccI CCATC 9 cut(s) 80, 401, 569, 721, 983, 1530, 1970, 2065, 2249
BceAI ACGGC 2 cut(s) 29, 791
BciT130I CCWGG 3 cut(s) 111, 948, 1882
BciVI GTATCC 1 cut(s) 1760
BclI TGATCA 2 cut(s) 697, 1957
BcnI CCSGG 1 cut(s) 803
BcoDI GTCTC 3 cut(s) 1432, 1454, 2132
BcuI ACTAGT 1 cut(s) 1477
BfaI CTAG 6 cut(s) 1082, 1191, 1478, 1598, 1904, 2163
BfmI CTRYAG 1 cut(s) 640
BfrI CTTAAG 1 cut(s) 1241
BfuI GTATCC 1 cut(s) 1760
BmcAI AGTACT 2 cut(s) 2147, 2182
Bme1390I CCNGG 4 cut(s) 111, 803, 948, 1882
Bme18I GGWCC 2 cut(s) 18, 89
BmeT110I CYCGRG 1 cut(s) 309
BmgT120I GGNCC 4 cut(s) 18, 89, 804, 1248
BmiI GGNNCC 3 cut(s) 20, 91, 1204
BmrFI CCNGG 4 cut(s) 111, 803, 948, 1882
BmrI ACTGGG 1 cut(s) 492
BmsI GCATC 1 cut(s) 1020
BmuI ACTGGG 1 cut(s) 492
BpmI CTGGAG 2 cut(s) 1251, 2115
Bpu14I TTCGAA 1 cut(s) 675
BpuEI CTTGAG 1 cut(s) 84
BpuMI CCSGG 1 cut(s) 803
BsaBI GATNNNNATC 1 cut(s) 1731
BsaJI CCNNGG 7 cut(s) 271, 324, 451, 508, 856, 1206, 1722
BsaWI WCCGGW 3 cut(s) 184, 239, 354
BsaXI ACNNNNNCTCC 2 cut(s) 1595, 1625
Bsc4I CCNNNNNNNGG 3 cut(s) 12, 184, 277
Bse118I RCCGGY 1 cut(s) 239
Bse1I ACTGG 5 cut(s) 487, 670, 1066, 1191, 1234
Bse21I CCTNAGG 1 cut(s) 1887
Bse3DI GCAATG 2 cut(s) 1116, 1932
Bse8I GATNNNNATC 1 cut(s) 1731
BseBI CCWGG 3 cut(s) 111, 948, 1882
BseDI CCNNGG 7 cut(s) 271, 324, 451, 508, 856, 1206, 1722
BseGI GGATG 4 cut(s) 551, 561, 1518, 2027
BseJI GATNNNNATC 1 cut(s) 1731
BseLI CCNNNNNNNGG 3 cut(s) 12, 184, 277
BseMI GCAATG 2 cut(s) 1116, 1932
BseMII CTCAG 2 cut(s) 1878, 2156
BseNI ACTGG 5 cut(s) 487, 670, 1066, 1191, 1234
BseRI GAGGAG 3 cut(s) 372, 1603, 1946
BseXI GCAGC 8 cut(s) 43, 92, 101, 239, 654, 696, 1589, 2232
BseYI CCCAGC 3 cut(s) 223, 364, 566
BsgI GTGCAG 1 cut(s) 940
Bsh1285I CGRYCG 1 cut(s) 185
BshFI GGCC 8 cut(s) 5, 44, 669, 806, 1250, 1885, 2006, 2050
BshTI ACCGGT 1 cut(s) 239
BsiEI CGRYCG 1 cut(s) 185
BsiHKAI GWGCWC 2 cut(s) 1518, 1597
BsiHKCI CYCGRG 1 cut(s) 309
BsiSI CCGG 4 cut(s) 185, 240, 355, 802
BslFI GGGAC 3 cut(s) 954, 1771, 2057
BslI CCNNNNNNNGG 3 cut(s) 12, 184, 277
BsmAI GTCTC 3 cut(s) 1432, 1454, 2132
BsmFI GGGAC 3 cut(s) 954, 1771, 2057
BsnI GGCC 8 cut(s) 5, 44, 669, 806, 1250, 1885, 2006, 2050
BsoBI CYCGRG 1 cut(s) 309
Bsp119I TTCGAA 1 cut(s) 675
Bsp1286I GDGCHC 2 cut(s) 1518, 1597
Bsp143I GATC 7 cut(s) 133, 697, 1125, 1541, 1726, 1936, 1957
Bsp19I CCATGG 1 cut(s) 324
BspACI CCGC 6 cut(s) 77, 213, 570, 1164, 1587, 1617
BspANI GGCC 8 cut(s) 5, 44, 669, 806, 1250, 1885, 2006, 2050
BspCNI CTCAG 2 cut(s) 1879, 2155
BspLI GGNNCC 3 cut(s) 20, 91, 1204
BspMAI CTGCAG 1 cut(s) 644
BspPI GGATC 3 cut(s) 1549, 1734, 1944
BspT104I TTCGAA 1 cut(s) 675
BspTI CTTAAG 1 cut(s) 1241
BsrBI CCGCTC 1 cut(s) 1619
BsrDI GCAATG 2 cut(s) 1116, 1932
BsrFI RCCGGY 1 cut(s) 239
BsrI ACTGG 5 cut(s) 487, 670, 1066, 1191, 1234
BssAI RCCGGY 1 cut(s) 239
BssECI CCNNGG 7 cut(s) 271, 324, 451, 508, 856, 1206, 1722
BssMI GATC 7 cut(s) 133, 697, 1125, 1541, 1726, 1936, 1957
BssT1I CCWWGG 6 cut(s) 271, 324, 451, 508, 856, 1722
Bst2UI CCWGG 3 cut(s) 111, 948, 1882
Bst4CI ACNGT 8 cut(s) 170, 589, 658, 1271, 1443, 1993, 2180, 2185
Bst6I CTCTTC 2 cut(s) 1887, 2130
BstAFI CTTAAG 1 cut(s) 1241
BstBI TTCGAA 1 cut(s) 675
BstC8I GCNNGC 4 cut(s) 343, 538, 772, 1909
BstDEI CTNAG 3 cut(s) 1771, 1887, 2142
BstDSI CCRYGG 2 cut(s) 324, 1206
BstEII GGTNACC 1 cut(s) 851
BstENI CCTNNNNNAGG 1 cut(s) 275
BstF5I GGATG 4 cut(s) 551, 561, 1518, 2027
BstHHI GCGC 1 cut(s) 536
BstKTI GATC 7 cut(s) 136, 700, 1128, 1544, 1729, 1939, 1960
BstMAI GTCTC 3 cut(s) 1432, 1454, 2132
BstMBI GATC 7 cut(s) 133, 697, 1125, 1541, 1726, 1936, 1957
BstMCI CGRYCG 1 cut(s) 185
BstMWI GCNNNNNNNGC 5 cut(s) 513, 776, 1290, 1586, 2012
BstNI CCWGG 3 cut(s) 111, 948, 1882
BstNSI RCATGY 2 cut(s) 493, 2003
BstPI GGTNACC 1 cut(s) 851
BstSCI CCNGG 4 cut(s) 109, 801, 946, 1880
BstSFI CTRYAG 1 cut(s) 640
BstV1I GCAGC 8 cut(s) 43, 92, 101, 239, 654, 696, 1589, 2232
BstXI CCANNNNNNTGG 1 cut(s) 372
Bsu36I CCTNAGG 1 cut(s) 1887
BsuI GTATCC 1 cut(s) 1760
BsuRI GGCC 8 cut(s) 5, 44, 669, 806, 1250, 1885, 2006, 2050
BtgI CCRYGG 2 cut(s) 324, 1206
BtgZI GCGATG 2 cut(s) 403, 2084
BtsCI GGATG 4 cut(s) 551, 561, 1518, 2027
BtsIMutI CAGTG 8 cut(s) 480, 526, 654, 823, 845, 1059, 1473, 1998
Cac8I GCNNGC 4 cut(s) 343, 538, 772, 1909
CfoI GCGC 1 cut(s) 536
Cfr10I RCCGGY 1 cut(s) 239
Cfr13I GGNCC 4 cut(s) 18, 89, 804, 1248
CseI GACGC 1 cut(s) 505
Csp6I GTAC 5 cut(s) 585, 944, 979, 2146, 2181
CspAI ACCGGT 1 cut(s) 239
CviQI GTAC 5 cut(s) 585, 944, 979, 2146, 2181
DdeI CTNAG 3 cut(s) 1771, 1887, 2142
DpnI GATC 7 cut(s) 135, 699, 1127, 1543, 1728, 1938, 1959
DpnII GATC 7 cut(s) 133, 697, 1125, 1541, 1726, 1936, 1957
DraIII CACNNNGTG 1 cut(s) 486
EaeI YGGCCR 3 cut(s) 3, 42, 2004
Eam1104I CTCTTC 2 cut(s) 1887, 2130
EarI CTCTTC 2 cut(s) 1887, 2130
EciI GGCGGA 1 cut(s) 202
Eco130I CCWWGG 6 cut(s) 271, 324, 451, 508, 856, 1722
Eco147I AGGCCT 2 cut(s) 1885, 2050
Eco47I GGWCC 2 cut(s) 18, 89
Eco81I CCTNAGG 1 cut(s) 1887
Eco88I CYCGRG 1 cut(s) 309
Eco91I GGTNACC 1 cut(s) 851
EcoNI CCTNNNNNAGG 1 cut(s) 275
EcoO109I RGGNCCY 1 cut(s) 18
EcoO65I GGTNACC 1 cut(s) 851
EcoRI GAATTC 2 cut(s) 188, 677
EcoRII CCWGG 3 cut(s) 109, 946, 1880
EcoT14I CCWWGG 6 cut(s) 271, 324, 451, 508, 856, 1722
ErhI CCWWGG 6 cut(s) 271, 324, 451, 508, 856, 1722
FalI AAGNNNNNCTT 2 cut(s) 1854, 1886
FaqI GGGAC 3 cut(s) 954, 1771, 2057
FauNDI CATATG 2 cut(s) 1762, 2248
FbaI TGATCA 2 cut(s) 697, 1957
FokI GGATG 4 cut(s) 548, 558, 1505, 2034
FspBI CTAG 6 cut(s) 1082, 1191, 1478, 1598, 1904, 2163
FspI TGCGCA 1 cut(s) 535
GlaI GCGC 1 cut(s) 535
GsaI CCCAGC 3 cut(s) 227, 368, 570
GsuI CTGGAG 2 cut(s) 1251, 2115
HaeIII GGCC 8 cut(s) 5, 44, 669, 806, 1250, 1885, 2006, 2050
HapII CCGG 4 cut(s) 185, 240, 355, 802
HgaI GACGC 1 cut(s) 505
HhaI GCGC 1 cut(s) 536
Hin6I GCGC 1 cut(s) 534
HinP1I GCGC 1 cut(s) 534
HincII GTYRAC 3 cut(s) 331, 799, 1972
HindII GTYRAC 3 cut(s) 331, 799, 1972
HindIII AAGCTT 1 cut(s) 1909
HpaII CCGG 4 cut(s) 185, 240, 355, 802
HphI GGTGA 8 cut(s) 143, 176, 287, 293, 697, 1447, 2151, 2210
Hpy166II GTNNAC 6 cut(s) 331, 799, 1306, 1483, 1972, 2159
Hpy188I TCNGA 9 cut(s) 182, 246, 400, 726, 929, 1696, 1703, 1989, 2170
Hpy188III TCNNGA 9 cut(s) 309, 971, 1072, 1196, 1495, 1710, 1955, 1961, 2132
Hpy8I GTNNAC 6 cut(s) 331, 799, 1306, 1483, 1972, 2159
HpyAV CCTTC 4 cut(s) 968, 1193, 1280, 2209
HpyCH4III ACNGT 8 cut(s) 170, 589, 658, 1271, 1443, 1993, 2180, 2185
HpyCH4IV ACGT 2 cut(s) 932, 1100
HpyF10VI GCNNNNNNNGC 5 cut(s) 513, 776, 1290, 1586, 2012
HpyF3I CTNAG 3 cut(s) 1771, 1887, 2142
HpySE526I ACGT 2 cut(s) 932, 1100
HspAI GCGC 1 cut(s) 534
Ksp22I TGATCA 2 cut(s) 697, 1957
Kzo9I GATC 7 cut(s) 133, 697, 1125, 1541, 1726, 1936, 1957
LmnI GCTCC 5 cut(s) 1208, 1381, 1592, 1703, 1846
Lsp1109I GCAGC 8 cut(s) 43, 92, 101, 239, 654, 696, 1589, 2232
LweI GCATC 1 cut(s) 1020
MaeI CTAG 6 cut(s) 1082, 1191, 1478, 1598, 1904, 2163
MaeII ACGT 2 cut(s) 932, 1100
MaeIII GTNAC 9 cut(s) 164, 173, 203, 431, 521, 827, 851, 1057, 2198
MalI GATC 7 cut(s) 135, 699, 1127, 1543, 1728, 1938, 1959
MbiI CCGCTC 1 cut(s) 1619
MboI GATC 7 cut(s) 133, 697, 1125, 1541, 1726, 1936, 1957
MboII GAAGA 7 cut(s) 479, 761, 1131, 1247, 1582, 1904, 2147
MfeI CAATTG 1 cut(s) 593
MhlI GDGCHC 2 cut(s) 1518, 1597
MlsI TGGCCA 2 cut(s) 5, 2006
MluNI TGGCCA 2 cut(s) 5, 2006
MlyI GAGTC 4 cut(s) 91, 326, 518, 2134
MmeI TCCRAC 2 cut(s) 205, 523
Mox20I TGGCCA 2 cut(s) 5, 2006
MroXI GAANNNNTTC 2 cut(s) 413, 955
MscI TGGCCA 2 cut(s) 5, 2006
MseI TTAA 8 cut(s) 236, 735, 810, 1242, 1392, 1790, 2033, 2240
MslI CAYNNNNRTG 4 cut(s) 1428, 1530, 1552, 1641
Msp20I TGGCCA 2 cut(s) 5, 2006
MspA1I CMGCKG 3 cut(s) 56, 83, 570
MspCI CTTAAG 1 cut(s) 1241
MspI CCGG 4 cut(s) 185, 240, 355, 802
MspR9I CCNGG 4 cut(s) 111, 803, 948, 1882
MunI CAATTG 1 cut(s) 593
MvaI CCWGG 3 cut(s) 111, 948, 1882
MwoI GCNNNNNNNGC 5 cut(s) 513, 776, 1290, 1586, 2012
NciI CCSGG 1 cut(s) 803
NcoI CCATGG 1 cut(s) 324
NdeI CATATG 2 cut(s) 1762, 2248
NdeII GATC 7 cut(s) 133, 697, 1125, 1541, 1726, 1936, 1957
NlaIV GGNNCC 3 cut(s) 20, 91, 1204
NmuCI GTSAC 6 cut(s) 164, 203, 431, 521, 1057, 2198
NsbI TGCGCA 1 cut(s) 535
NspI RCATGY 2 cut(s) 493, 2003
NspV TTCGAA 1 cut(s) 675
PceI AGGCCT 2 cut(s) 1885, 2050
PciI ACATGT 1 cut(s) 1999
PcsI WCGNNNNNNNCGW 1 cut(s) 933
PdmI GAANNNNTTC 2 cut(s) 413, 955
PfeI GAWTC 6 cut(s) 414, 862, 1275, 1717, 1795, 1945
PflFI GACNNNGTC 1 cut(s) 1967
PinAI ACCGGT 1 cut(s) 239
PleI GAGTC 4 cut(s) 91, 326, 518, 2134
PpsI GAGTC 4 cut(s) 91, 326, 518, 2134
PpuMI RGGWCCY 1 cut(s) 18
PscI ACATGT 1 cut(s) 1999
PshBI ATTAAT 1 cut(s) 735
PsiI TTATAA 1 cut(s) 623
Psp5II RGGWCCY 1 cut(s) 18
Psp6I CCWGG 3 cut(s) 109, 946, 1880
PspEI GGTNACC 1 cut(s) 851
PspFI CCCAGC 3 cut(s) 223, 364, 566
PspGI CCWGG 3 cut(s) 109, 946, 1880
PspN4I GGNNCC 3 cut(s) 20, 91, 1204
PspPI GGNCC 4 cut(s) 18, 89, 804, 1248
PspPPI RGGWCCY 1 cut(s) 18
PstI CTGCAG 1 cut(s) 644
PsyI GACNNNGTC 1 cut(s) 1967
PvuII CAGCTG 2 cut(s) 56, 83
RsaI GTAC 5 cut(s) 586, 945, 980, 2147, 2182
RsaNI GTAC 5 cut(s) 585, 944, 979, 2146, 2181
RseI CAYNNNNRTG 4 cut(s) 1428, 1530, 1552, 1641
SaqAI TTAA 8 cut(s) 236, 735, 810, 1242, 1392, 1790, 2033, 2240
Sau3AI GATC 7 cut(s) 133, 697, 1125, 1541, 1726, 1936, 1957
Sau96I GGNCC 4 cut(s) 18, 89, 804, 1248
ScaI AGTACT 2 cut(s) 2147, 2182
SchI GAGTC 4 cut(s) 91, 326, 518, 2134
ScrFI CCNGG 4 cut(s) 111, 803, 948, 1882
SduI GDGCHC 2 cut(s) 1518, 1597
SfaNI GCATC 1 cut(s) 1020
SfcI CTRYAG 1 cut(s) 640
SfuI TTCGAA 1 cut(s) 675
SinI GGWCC 2 cut(s) 18, 89
SmiMI CAYNNNNRTG 4 cut(s) 1428, 1530, 1552, 1641
SmlI CTYRAG 2 cut(s) 99, 1241
SmoI CTYRAG 2 cut(s) 99, 1241
SpeI ACTAGT 1 cut(s) 1477
SseBI AGGCCT 2 cut(s) 1885, 2050
SsiI CCGC 6 cut(s) 77, 213, 570, 1164, 1587, 1617
SspMI CTAG 6 cut(s) 1082, 1191, 1478, 1598, 1904, 2163
StuI AGGCCT 2 cut(s) 1885, 2050
StyD4I CCNGG 4 cut(s) 109, 801, 946, 1880
StyI CCWWGG 6 cut(s) 271, 324, 451, 508, 856, 1722
TaaI ACNGT 8 cut(s) 170, 589, 658, 1271, 1443, 1993, 2180, 2185
TaiI ACGT 2 cut(s) 935, 1103
TaqI TCGA 2 cut(s) 660, 675
TatI WGTACW 2 cut(s) 2145, 2180
TauI GCSGC 2 cut(s) 80, 1167
TfiI GAWTC 6 cut(s) 414, 862, 1275, 1717, 1795, 1945
Tru1I TTAA 8 cut(s) 236, 735, 810, 1242, 1392, 1790, 2033, 2240
Tru9I TTAA 8 cut(s) 236, 735, 810, 1242, 1392, 1790, 2033, 2240
TscAI CASTG 8 cut(s) 487, 526, 661, 823, 845, 1066, 1473, 1998
TseFI GTSAC 6 cut(s) 164, 203, 431, 521, 1057, 2198
TseI GCWGC 8 cut(s) 56, 80, 114, 227, 642, 684, 1577, 2220
Tsp45I GTSAC 6 cut(s) 164, 203, 431, 521, 1057, 2198
TspDTI ATGAA 9 cut(s) 292, 406, 466, 1038, 1083, 1480, 1542, 2072, 2097
TspGWI ACGGA 3 cut(s) 242, 1010, 1195
TspRI CASTG 8 cut(s) 487, 526, 661, 823, 845, 1066, 1473, 1998
Tth111I GACNNNGTC 1 cut(s) 1967
Vha464I CTTAAG 1 cut(s) 1241
VpaK11BI GGWCC 2 cut(s) 18, 89
VspI ATTAAT 1 cut(s) 735
XagI CCTNNNNNAGG 1 cut(s) 275
XapI RAATTY 5 cut(s) 188, 263, 677, 1089, 1950
XceI RCATGY 2 cut(s) 493, 2003
XcmI CCANNNNNNNNNTGG 1 cut(s) 1529
XmnI GAANNNNTTC 2 cut(s) 413, 955
XspI CTAG 6 cut(s) 1082, 1191, 1478, 1598, 1904, 2163
ZrmI AGTACT 2 cut(s) 2147, 2182
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.