Rmu_sc0008053.1_g000050

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0008053.1
OG0000066
Physical Location & Seq
Forward (+)
170951 .. 173876
2926 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0008053.1_g000050.1.cds

Sequence Viewer

Length: 2289 bp
atgtcgatcatggccttgcaattcccattggcggcagtcctaatcctattattagcggctagtactacgacaacagcagctgctgctcaagccctcccaactgactgcccagacaagtgcggcaatctcacaattccatatccttttggcatgaaggagggttgttacctgcgaaaagaattctttatcaattgtagccataccacccaaccaccaacaccatatttgatgcatgggaatgtcgttgttactaacatttcccttgatgaaggtgagttgcgaatgttgcactatgtagggcgagattgttacgatgcatatggtcgaactaacaccgaccctcggctcaccctgcctcttccttatacgatctctgatactaaaaacatattcactgtcgttggttgtgacacttccgcaactttccaaggctaccgaggggaaaaaagtcagccgttctttactgggtgcatctcagtatgcagcagccttggcagtgttgataacaactcttgctctggggttgggtgttgccagactaacatccccagtggactgcacaatcgtactactgtgcgtttggctagctactacaatcattcgtatgtgttggattttaacccctgcagctacgccttcattgcagagaaaggccaattcaatttcacctcttctagttttgaacaactgaatggcactgaacagcttccagtggttctcaattgggccattgggaatgacacagacccctgtgatgaagctcaacatcgggaggattttgcatgcatggctaatagcaattgcataaaccgtcatattgatgggtctgtaggttacttatgccagtgcaagcctggttatgaaggaaacccgtaccacccagatggttgccaagatattgatgagtgcagggctctcaagccctgccagaacggacggtgcttaaatactccaggaaatcactcttgttcatgtcacaaaggatacaaaaacgatggcatgaatgacaagacttgcatcaaagacgattccgaaacacacaaaaagatcgttctcctgcttatggtttcattgggtgtaactgtgggcttcttggttttattccttggaatttcgtggacatgttggggaatgaagaaaagagagttcattaaactcaaagaaaagcacttcaaagagaatggtggcttattgttactgcaacaactcgatcgtcatggaagctcgatgaggacaacaaaaatctttcctactgaagaacttgagaaggcaacaaacaactaccatgagagtagagtccttggagaaggaggttatggaacagtttacaaaggaatactaccggatgacacaatggttgccataaagaagtcgaaaggtggtgccctgacccagagtgatcaatttgttaacgaggtgattgttctttctcaaatcaaccacaaaaatgtggtaaagctattgggttgttgtttagaaacggaagtacctttactagtatacgaattcattacccatggcactctttatgagcacattcataaaaaaggatcatcactcccatttgaattacgaatgaagatagcaactcaaagcgcggaagcactgtcccacttacactcctcaatttccacaccaataatacatcgagatgtgaaaacagcaaatattctgttagatgatgattatacagcaaaagtgtcagattttggagcttcccgattggttccttcaggtcaaactgatatacaaactttagtgcttgggacatttggatacctggaccctgaatatctgcaatcaaaccaactaacagaaaagagtgatgtttacagctttggagttgtcctagtggagctactatcaagcaaaatgccggtttctaaagatagatgcttagcaagcatctttcttgcttccatggaagaaggttggttgaatcaaatacttgatgatggcatagtgaatgagggaaacattgagacggtaaagaaagtggccaatctggcaaaacgatgcttgagggtaaacggggaggaaaggcctaccatgaaagaagttgtgaaagagttagaggaaatgagtgtcacggcaaaacatccgtggggatttaatgctaatttttgcgaagaagagactgaatacttgcttgggtcaattaattcagacgcttacgttgtgggtaatggagaaggtgattgcagtcctagtggtctaaccggtggtacgaccactgcgtatgacagcatgcaaatcgaacagttaatgccacatgatggtggacgatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK

Protein Analysis

762

Amino Acids

83.99

Weight (kDa)

5.52

Isoelectric Point (pI)

35.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21210 AT1G21230 AT1G21240 AT1G21240 AT1G21245 AT1G21250 AT1G21270 AT1G22720 AT3G25490
fragaria_vesca FvH4_2g35051 FvH4_2g35051 FvH4_2g35060 FvH4_3g10281 FvH4_3g10285 FvH4_3g10286 FvH4_3g10311 FvH4_3g10311 FvH4_3g10320 FvH4_3g10332 FvH4_3g10340 FvH4_3g10350 FvH4_3g10360 FvH4_3g10390 FvH4_3g10412 FvH4_3g10413 FvH4_3g10461 FvH4_3g10462 FvH4_3g10470 FvH4_3g10510 FvH4_3g10520 FvH4_3g10530 FvH4_3g10550 FvH4_3g10560 FvH4_3g10611 FvH4_3g11730 FvH4_6g53140 FvH4_6g53142 FvH4_7g31880 FvH4_7g31910 FvH4_7g31920
malus_domestica MD05G1269900.v1.1 MD05G1270000.v1.1 MD05G1270200.v1.1 MD05G1270400.v1.1 MD05G1270500.v1.1 MD05G1270600.v1.1 MD05G1270800.v1.1 MD05G1270900.v1.1 MD07G1183800.v1.1 MD08G1107600.v1.1 MD10G1248500.v1.1 MD10G1249200.v1.1 MD10G1249500.v1.1 MD10G1250000.v1.1 MD10G1250500.v1.1 MD10G1250900.v1.1 MD10G1251200.v1.1 MD10G1251400.v1.1 MD13G1004600.v1.1 MD15G1088400.v1.1
prunus_persica Prupe.1G442400_v2.0.a1 Prupe.1G442500_v2.0.a1 Prupe.2G223400_v2.0.a1 Prupe.4G091900_v2.0.a1 Prupe.4G093200_v2.0.a1 Prupe.4G093300_v2.0.a1 Prupe.4G093400_v2.0.a1 Prupe.4G093500_v2.0.a1 Prupe.4G094100_v2.0.a1 Prupe.4G094200_v2.0.a1 Prupe.4G094300_v2.0.a1 Prupe.6G358300_v2.0.a1
pyrus_communis pycom08g08990 pycom10g20660 pycom10g20670 pycom10g20680 pycom10g20710 pycom10g20740 pycom13g00400 pycom15g08290
rosa_chinensis RchiOBHm_Chr1g0326861 RchiOBHm_Chr1g0326871 RchiOBHm_Chr1g0331391 RchiOBHm_Chr1g0336911 RchiOBHm_Chr1g0364001 RchiOBHm_Chr2g0174541 RchiOBHm_Chr2g0175041 RchiOBHm_Chr2g0175101 RchiOBHm_Chr2g0175121 RchiOBHm_Chr3g0486331 RchiOBHm_Chr5g0015581 RchiOBHm_Chr5g0016551 RchiOBHm_Chr5g0016561 RchiOBHm_Chr5g0016581 RchiOBHm_Chr5g0016601 RchiOBHm_Chr5g0016611 RchiOBHm_Chr5g0016641 RchiOBHm_Chr5g0016661 RchiOBHm_Chr5g0016691 RchiOBHm_Chr5g0016701 RchiOBHm_Chr5g0016731 RchiOBHm_Chr5g0016741 RchiOBHm_Chr5g0016811 RchiOBHm_Chr5g0016841 RchiOBHm_Chr5g0016871 RchiOBHm_Chr5g0016881 RchiOBHm_Chr5g0016931 RchiOBHm_Chr5g0017021 RchiOBHm_Chr5g0017041 RchiOBHm_Chr5g0017071 RchiOBHm_Chr5g0017091 RchiOBHm_Chr5g0017121 RchiOBHm_Chr5g0017141 RchiOBHm_Chr5g0017291 RchiOBHm_Chr5g0017321 RchiOBHm_Chr5g0017401 RchiOBHm_Chr5g0019001 RchiOBHm_Chr5g0019071 RchiOBHm_Chr5g0028941 RchiOBHm_Chr5g0052141 RchiOBHm_Chr5g0054721 RchiOBHm_Chr5g0054801 RchiOBHm_Chr5g0054931 RchiOBHm_Chr5g0054941 RchiOBHm_Chr6g0259291 RchiOBHm_Chr6g0292991 RchiOBHm_Chr6g0306911
rosa_laevigata RLG00000009465 RLG00000009468 RLG00000010755 RLG00000011976 RLG00000014541 RLG00000014542 RLG00000015169 RLG00000022308 RLG00000027529 RLG00000029324 RLG00000030244 RLG00000030245 RLG00000032210 RLG00000032211 RLG00000032257 RLG00000032259 RLG00000032260 RLG00000032261 RLG00000032262 RLG00000032268 RLG00000032269 RLG00000032272 RLG00000032282 RLG00000032283 RLG00000032308 RLG00000032413 RLG00000033129 RLG00000034945
rosa_multiflora Rmu_co8241929.1_g000001 Rmu_co8269227.1_g000001 Rmu_co8461259.1_g000001 Rmu_co8495385.1_g000001 Rmu_sc0000028.1_g000003 Rmu_sc0000083.1_g000009 Rmu_sc0000083.1_g000010 Rmu_sc0000083.1_g000031 Rmu_sc0000083.1_g000043 Rmu_sc0000083.1_g000046 Rmu_sc0000083.1_g000047 Rmu_sc0000563.1_g000024 Rmu_sc0000842.1_g000012 Rmu_sc0001235.1_g000001 Rmu_sc0001235.1_g000005 Rmu_sc0001235.1_g000009 Rmu_sc0001235.1_g000015 Rmu_sc0001235.1_g000016 Rmu_sc0001235.1_g000017 Rmu_sc0001473.1_g000036 Rmu_sc0001607.1_g000016 Rmu_sc0001788.1_g000006 Rmu_sc0002018.1_g000008 Rmu_sc0002028.1_g000001 Rmu_sc0002680.1_g000011 Rmu_sc0002798.1_g000023 Rmu_sc0002886.1_g000023 Rmu_sc0002989.1_g000012 Rmu_sc0003064.1_g000009 Rmu_sc0003382.1_g000006 Rmu_sc0003630.1_g000002 Rmu_sc0003630.1_g000047 Rmu_sc0003630.1_g000066 Rmu_sc0003630.1_g000067 Rmu_sc0003649.1_g000010 Rmu_sc0003688.1_g000005 Rmu_sc0003920.1_g000007 Rmu_sc0004765.1_g000007 Rmu_sc0004765.1_g000008 Rmu_sc0004920.1_g000011 Rmu_sc0005223.1_g000008 Rmu_sc0005223.1_g000010 Rmu_sc0005223.1_g000015 Rmu_sc0005223.1_g000016 Rmu_sc0005319.1_g000014 Rmu_sc0005319.1_g000015 Rmu_sc0005319.1_g000018 Rmu_sc0005319.1_g000019 Rmu_sc0005319.1_g000025 Rmu_sc0005418.1_g000007 Rmu_sc0005418.1_g000012 Rmu_sc0006106.1_g000011 Rmu_sc0006914.1_g000012 Rmu_sc0007023.1_g000001 Rmu_sc0007662.1_g000001 Rmu_sc0007920.1_g000002 Rmu_sc0007920.1_g000013 Rmu_sc0008053.1_g000016 Rmu_sc0008053.1_g000017 Rmu_sc0008053.1_g000018 Rmu_sc0008053.1_g000023 Rmu_sc0008053.1_g000050 Rmu_sc0009042.1_g000007 Rmu_sc0009042.1_g000008 Rmu_sc0010642.1_g000008 Rmu_sc0011109.1_g000002 Rmu_sc0011314.1_g000006 Rmu_sc0011974.1_g000006 Rmu_sc0012179.1_g000009 Rmu_sc0015169.1_g000001 Rmu_sc0016368.1_g000002 Rmu_sc0022659.1_g000001 Rmu_sc0027371.1_g000001 Rmu_sc0028213.1_g000001 Rmu_sc0034526.1_g000001 Rmu_sc0039366.1_g000002 Rmu_ssc0000050.1_g000084 Rmu_ssc0000050.1_g000088 Rmu_ssc0000167.1_g000017
rosa_roxburghii Rroxscaffold_1G00011340 Rroxscaffold_1G00025710 Rroxscaffold_1G00025870 Rroxscaffold_1G00026070 Rroxscaffold_1G00026170 Rroxscaffold_1G00026200 Rroxscaffold_1G00026270 Rroxscaffold_1G00050950 Rroxscaffold_1G00058900 Rroxscaffold_1G00060040 Rroxscaffold_1G00060060 Rroxscaffold_1G00060070 Rroxscaffold_1G00060080 Rroxscaffold_1G00060110 Rroxscaffold_1G00060120 Rroxscaffold_1G00060140 Rroxscaffold_1G00060170 Rroxscaffold_1G00060210 Rroxscaffold_1G00060220 Rroxscaffold_1G00060240 Rroxscaffold_1G00060250 Rroxscaffold_1G00060260 Rroxscaffold_1G00060300 Rroxscaffold_1G00060320 Rroxscaffold_1G00060330 Rroxscaffold_1G00060900 Rroxscaffold_1G00060920 Rroxscaffold_1G00073980 Rroxscaffold_2G00077560 Rroxscaffold_2G00077580 Rroxscaffold_2G00146990 Rroxscaffold_3G00236200 Rroxscaffold_3G00236210 Rroxscaffold_3G00236220 Rroxscaffold_4G00292760 Rroxscaffold_4G00315200 Rroxscaffold_4G00315240 Rroxscaffold_4G00326240 Rroxscaffold_4G00328980 Rroxscaffold_5G00341890 Rroxscaffold_7G00161350 Rroxscaffold_7G00174490 Rroxscaffold_7G00206550
rosa_rugosa Rorug01G0093000 Rorug01G0093100 Rorug01G0128800 Rorug01G0312000 Rorug01G0312000 Rorug02G0581100 Rorug03G0224600 Rorug05G0028500 Rorug05G0032700 Rorug05G0032800 Rorug05G0032900 Rorug05G0032900 Rorug05G0033000 Rorug05G0033100 Rorug05G0033300 Rorug05G0033400 Rorug05G0033900 Rorug05G0034900 Rorug05G0035000 Rorug05G0046900 Rorug05G0047500 Rorug05G0047500 Rorug05G0111600 Rorug05G0287900 Rorug05G0288700 Rorug05G0586900 Rorug06G0231700 Rorug06G0354300 Rorug06G0354400
rosa_samantha Rh1DG122200 Rh2DG686800 Rh5BG136600 Rh6AG344500 Rh6BG351900 Rh6CG359000 Rh6CG359100 Rh6CG387600 Rh6CG480500 Rh6DG344600 Rh6DG467400
rosa_wichuraiana Rw0G010250 Rw0G014820 Rw0G021400 Rw0G023100 Rw1G009450 Rw1G012100 Rw1G012180 Rw1G028360 Rw2G054260 Rw3G024370 Rw3G024420 Rw3G024470 Rw5G010850 Rw5G011010 Rw5G011020 Rw5G011050 Rw5G011080 Rw5G011120 Rw5G012300 Rw5G018470 Rw5G033830 Rw6G008930 Rw6G030070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 177
AccB1I GGYRCC 1 cut(s) 1382
AccB7I CCANNNNNTGG 1 cut(s) 2276
AccI GTMKAC 1 cut(s) 1500
AccII CGCG 1 cut(s) 1598
AciI CCGC 5 cut(s) 32, 56, 120, 417, 1598
AclWI GGATC 1 cut(s) 1558
AcoI YGGCCR 1 cut(s) 1998
AcsI RAATTY 3 cut(s) 179, 1110, 1505
AcuI CTGAAG 2 cut(s) 1275, 1716
AfaI GTAC 5 cut(s) 64, 568, 875, 1488, 2227
AfiI CCNNNNNNNGG 4 cut(s) 31, 342, 1063, 2276
AflIII ACRYGT 1 cut(s) 1121
AgeI ACCGGT 1 cut(s) 2219
AgsI TTSAA 5 cut(s) 661, 683, 1174, 1568, 1939
AhlI ACTAGT 1 cut(s) 1495
AjnI CCWGG 3 cut(s) 853, 952, 1779
AjuI GAANNNNNNNTTGG 2 cut(s) 2133, 2165
Alw21I GWGCWC 1 cut(s) 1536
Alw26I GTCTC 2 cut(s) 1976, 2129
AlwI GGATC 1 cut(s) 1558
AlwNI CAGNNNCTG 2 cut(s) 80, 83
AoxI GGCC 5 cut(s) 12, 652, 726, 1998, 2042
ApeKI GCWGC 6 cut(s) 77, 80, 83, 483, 486, 627
ApoI RAATTY 3 cut(s) 179, 1110, 1505
AseI ATTAAT 1 cut(s) 2160
AsiGI ACCGGT 1 cut(s) 2219
Asp700I GAANNNNTTC 1 cut(s) 705
AspLEI GCGC 1 cut(s) 1598
AspS9I GGNCC 2 cut(s) 726, 1783
AsuHPI GGTGA 5 cut(s) 284, 340, 658, 1429, 2207
AsuNHI GCTAGC 1 cut(s) 584
AvaII GGWCC 1 cut(s) 1783
BaeGI GKGCMC 1 cut(s) 1387
BaeI ACNNNNGTAYC 2 cut(s) 1470, 1503
BalI TGGCCA 1 cut(s) 2000
BanI GGYRCC 1 cut(s) 1382
BanII GRGCYC 1 cut(s) 916
BarI GAAGNNNNNNTAC 2 cut(s) 1476, 1508
Bbv12I GWGCWC 1 cut(s) 1536
BbvI GCAGC 6 cut(s) 67, 70, 89, 495, 498, 639
BccI CCATC 5 cut(s) 815, 878, 989, 1949, 2270
BceAI ACGGC 2 cut(s) 439, 2106
BcgI CGANNNNNNTGC 2 cut(s) 2236, 2270
BciT130I CCWGG 3 cut(s) 855, 954, 1781
BciVI GTATCC 2 cut(s) 977, 1769
BclI TGATCA 1 cut(s) 1399
BcoDI GTCTC 2 cut(s) 1976, 2129
BcuI ACTAGT 1 cut(s) 1495
BfaI CTAG 6 cut(s) 60, 585, 675, 1496, 1850, 2208
BfmI CTRYAG 2 cut(s) 625, 828
BfuAI ACCTGC 1 cut(s) 177
BfuI GTATCC 2 cut(s) 977, 1769
BglI GCCNNNNNGGC 1 cut(s) 2006
BisI GCNGC 9 cut(s) 33, 57, 78, 81, 84, 121, 484, 487, 628
BlpI GCTNAGC 1 cut(s) 1897
BlsI GCNGC 9 cut(s) 34, 58, 79, 82, 85, 122, 485, 488, 629
BmcAI AGTACT 1 cut(s) 64
Bme1390I CCNGG 3 cut(s) 855, 954, 1781
Bme18I GGWCC 1 cut(s) 1783
BmgT120I GGNCC 2 cut(s) 726, 1783
BmiI GGNNCC 3 cut(s) 1384, 1728, 1785
BmrFI CCNGG 3 cut(s) 855, 954, 1781
BmrI ACTGGG 2 cut(s) 474, 543
BmsI GCATC 7 cut(s) 219, 304, 480, 1026, 1883, 1914, 2006
BmtI GCTAGC 1 cut(s) 588
BmuI ACTGGG 2 cut(s) 474, 543
BpmI CTGGAG 1 cut(s) 936
Bpu1102I GCTNAGC 1 cut(s) 1897
BpuEI CTTGAG 4 cut(s) 72, 902, 1283, 2041
BsaJI CCNNGG 9 cut(s) 341, 427, 436, 490, 1105, 1300, 1516, 1920, 2102
BsaWI WCCGGW 2 cut(s) 1342, 2219
BsaXI ACNNNNNCTCC 2 cut(s) 1604, 1634
Bsc4I CCNNNNNNNGG 4 cut(s) 31, 342, 1063, 2276
Bse118I RCCGGY 2 cut(s) 1876, 2219
Bse1I ACTGG 4 cut(s) 469, 549, 710, 844
Bse3DI GCAATG 1 cut(s) 639
BseBI CCWGG 3 cut(s) 855, 954, 1781
BseDI CCNNGG 9 cut(s) 341, 427, 436, 490, 1105, 1300, 1516, 1920, 2102
BseGI GGATG 3 cut(s) 543, 1351, 2098
BseLI CCNNNNNNNGG 4 cut(s) 31, 342, 1063, 2276
BseMI GCAATG 1 cut(s) 639
BseMII CTCAG 1 cut(s) 489
BseNI ACTGG 4 cut(s) 469, 549, 710, 844
BseRI GAGGAG 1 cut(s) 1612
BseSI GKGCMC 1 cut(s) 1387
BseXI GCAGC 6 cut(s) 67, 70, 89, 495, 498, 639
BsgI GTGCAG 2 cut(s) 542, 928
Bsh1236I CGCG 1 cut(s) 1598
Bsh1285I CGRYCG 1 cut(s) 1213
BshFI GGCC 5 cut(s) 14, 654, 728, 2000, 2044
BshNI GGYRCC 1 cut(s) 1382
BshTI ACCGGT 1 cut(s) 2219
BsiEI CGRYCG 1 cut(s) 1213
BsiHKAI GWGCWC 1 cut(s) 1536
BsiSI CCGG 3 cut(s) 1343, 1877, 2220
BslFI GGGAC 2 cut(s) 1594, 1780
BslI CCNNNNNNNGG 4 cut(s) 31, 342, 1063, 2276
BsmAI GTCTC 2 cut(s) 1976, 2129
BsmBI CGTCTC 1 cut(s) 1976
BsmFI GGGAC 2 cut(s) 1594, 1780
BsnI GGCC 5 cut(s) 14, 654, 728, 2000, 2044
Bsp1286I GDGCHC 3 cut(s) 916, 1387, 1536
Bsp143I GATC 6 cut(s) 6, 369, 1047, 1210, 1399, 1550
Bsp1720I GCTNAGC 1 cut(s) 1897
Bsp19I CCATGG 2 cut(s) 1516, 1920
BspACI CCGC 5 cut(s) 32, 56, 120, 417, 1598
BspANI GGCC 5 cut(s) 14, 654, 728, 2000, 2044
BspCNI CTCAG 1 cut(s) 488
BspFNI CGCG 1 cut(s) 1598
BspLI GGNNCC 3 cut(s) 1384, 1728, 1785
BspMAI CTGCAG 1 cut(s) 629
BspMI ACCTGC 1 cut(s) 177
BspOI GCTAGC 1 cut(s) 588
BspPI GGATC 1 cut(s) 1558
BspT107I GGYRCC 1 cut(s) 1382
BsrDI GCAATG 1 cut(s) 639
BsrFI RCCGGY 2 cut(s) 1876, 2219
BsrI ACTGG 4 cut(s) 469, 549, 710, 844
BssAI RCCGGY 2 cut(s) 1876, 2219
BssECI CCNNGG 9 cut(s) 341, 427, 436, 490, 1105, 1300, 1516, 1920, 2102
BssMI GATC 6 cut(s) 6, 369, 1047, 1210, 1399, 1550
BssNAI GTATAC 1 cut(s) 1501
BssT1I CCWWGG 6 cut(s) 427, 490, 1105, 1300, 1516, 1920
Bst1107I GTATAC 1 cut(s) 1501
Bst2UI CCWGG 3 cut(s) 855, 954, 1781
Bst4CI ACNGT 9 cut(s) 397, 574, 812, 939, 1084, 1324, 1608, 1987, 2262
Bst6I CTCTTC 3 cut(s) 363, 676, 2127
BstAPI GCANNNNNTGC 1 cut(s) 83
BstC8I GCNNGC 5 cut(s) 586, 784, 851, 1903, 2249
BstDEI CTNAG 2 cut(s) 475, 1897
BstDSI CCRYGG 3 cut(s) 1516, 1920, 2102
BstF5I GGATG 3 cut(s) 543, 1351, 2098
BstFNI CGCG 1 cut(s) 1598
BstHHI GCGC 1 cut(s) 1598
BstKTI GATC 6 cut(s) 9, 372, 1050, 1213, 1402, 1553
BstMAI GTCTC 2 cut(s) 1976, 2129
BstMBI GATC 6 cut(s) 6, 369, 1047, 1210, 1399, 1550
BstMCI CGRYCG 1 cut(s) 1213
BstMWI GCNNNNNNNGC 9 cut(s) 83, 89, 286, 352, 492, 641, 788, 1902, 2006
BstNI CCWGG 3 cut(s) 855, 954, 1781
BstNSI RCATGY 3 cut(s) 786, 1125, 2251
BstSCI CCNGG 3 cut(s) 853, 952, 1779
BstSFI CTRYAG 2 cut(s) 625, 828
BstSLI GKGCMC 1 cut(s) 1387
BstUI CGCG 1 cut(s) 1598
BstV1I GCAGC 6 cut(s) 67, 70, 89, 495, 498, 639
BstXI CCANNNNNNTGG 1 cut(s) 884
BstZ17I GTATAC 1 cut(s) 1501
BsuI GTATCC 2 cut(s) 977, 1769
BsuRI GGCC 5 cut(s) 14, 654, 728, 2000, 2044
BtgI CCRYGG 3 cut(s) 1516, 1920, 2102
BtsCI GGATG 3 cut(s) 543, 1351, 2098
BtsI GCAGTG 2 cut(s) 502, 2232
BtsIMutI CAGTG 8 cut(s) 393, 502, 556, 696, 717, 851, 1604, 2232
BveI ACCTGC 1 cut(s) 177
Cac8I GCNNGC 5 cut(s) 586, 784, 851, 1903, 2249
CaiI CAGNNNCTG 2 cut(s) 80, 83
CfoI GCGC 1 cut(s) 1598
Cfr10I RCCGGY 2 cut(s) 1876, 2219
Cfr13I GGNCC 2 cut(s) 726, 1783
CseI GACGC 1 cut(s) 2177
Csp6I GTAC 5 cut(s) 63, 567, 874, 1487, 2226
CspAI ACCGGT 1 cut(s) 2219
CviQI GTAC 5 cut(s) 63, 567, 874, 1487, 2226
DdeI CTNAG 2 cut(s) 475, 1897
DpnI GATC 6 cut(s) 8, 371, 1049, 1212, 1401, 1552
DpnII GATC 6 cut(s) 6, 369, 1047, 1210, 1399, 1550
EaeI YGGCCR 1 cut(s) 1998
Eam1104I CTCTTC 3 cut(s) 363, 676, 2127
EarI CTCTTC 3 cut(s) 363, 676, 2127
Eco130I CCWWGG 6 cut(s) 427, 490, 1105, 1300, 1516, 1920
Eco147I AGGCCT 1 cut(s) 2044
Eco24I GRGCYC 1 cut(s) 916
Eco47I GGWCC 1 cut(s) 1783
Eco57I CTGAAG 2 cut(s) 1275, 1716
EcoRI GAATTC 2 cut(s) 179, 1505
EcoRII CCWGG 3 cut(s) 853, 952, 1779
EcoT14I CCWWGG 6 cut(s) 427, 490, 1105, 1300, 1516, 1920
EcoT22I ATGCAT 3 cut(s) 234, 319, 788
EcoT38I GRGCYC 1 cut(s) 916
ErhI CCWWGG 6 cut(s) 427, 490, 1105, 1300, 1516, 1920
Esp3I CGTCTC 1 cut(s) 1976
FaqI GGGAC 2 cut(s) 1594, 1780
FauNDI CATATG 1 cut(s) 319
FbaI TGATCA 1 cut(s) 1399
FblI GTMKAC 1 cut(s) 1500
Fnu4HI GCNGC 9 cut(s) 33, 57, 78, 81, 84, 121, 484, 487, 628
FokI GGATG 3 cut(s) 530, 1358, 2085
FriOI GRGCYC 1 cut(s) 916
Fsp4HI GCNGC 9 cut(s) 33, 57, 78, 81, 84, 121, 484, 487, 628
FspBI CTAG 6 cut(s) 60, 585, 675, 1496, 1850, 2208
GlaI GCGC 1 cut(s) 1597
GluI GCNGC 9 cut(s) 33, 57, 78, 81, 84, 121, 484, 487, 628
GsuI CTGGAG 1 cut(s) 936
HaeIII GGCC 5 cut(s) 14, 654, 728, 2000, 2044
HapII CCGG 3 cut(s) 1343, 1877, 2220
HgaI GACGC 1 cut(s) 2177
HhaI GCGC 1 cut(s) 1598
Hin6I GCGC 1 cut(s) 1596
HinP1I GCGC 1 cut(s) 1596
HincII GTYRAC 1 cut(s) 1411
HindII GTYRAC 1 cut(s) 1411
HinfI GANTC 3 cut(s) 1028, 1296, 1939
HpaI GTTAAC 1 cut(s) 1411
HpaII CCGG 3 cut(s) 1343, 1877, 2220
HphI GGTGA 5 cut(s) 284, 340, 658, 1429, 2207
Hpy166II GTNNAC 8 cut(s) 554, 1119, 1327, 1411, 1501, 1831, 2029, 2282
Hpy188I TCNGA 4 cut(s) 376, 1033, 1705, 2167
Hpy188III TCNNGA 3 cut(s) 770, 1649, 1719
Hpy8I GTNNAC 8 cut(s) 554, 1119, 1327, 1411, 1501, 1831, 2029, 2282
HpyAV CCTTC 9 cut(s) 148, 263, 646, 857, 1261, 1301, 1740, 1922, 2186
HpyCH4III ACNGT 9 cut(s) 397, 574, 812, 939, 1084, 1324, 1608, 1987, 2262
HpyCH4IV ACGT 1 cut(s) 2175
HpyF10VI GCNNNNNNNGC 9 cut(s) 83, 89, 286, 352, 492, 641, 788, 1902, 2006
HpyF3I CTNAG 2 cut(s) 475, 1897
HpySE526I ACGT 1 cut(s) 2175
HspAI GCGC 1 cut(s) 1596
Ksp22I TGATCA 1 cut(s) 1399
KspAI GTTAAC 1 cut(s) 1411
Kzo9I GATC 6 cut(s) 6, 369, 1047, 1210, 1399, 1550
LmnI GCTCC 2 cut(s) 1712, 1855
Lsp1109I GCAGC 6 cut(s) 67, 70, 89, 495, 498, 639
LweI GCATC 7 cut(s) 219, 304, 480, 1026, 1883, 1914, 2006
MaeI CTAG 6 cut(s) 60, 585, 675, 1496, 1850, 2208
MaeII ACGT 1 cut(s) 2175
MaeIII GTNAC 9 cut(s) 164, 247, 308, 407, 833, 974, 1078, 1194, 2086
MalI GATC 6 cut(s) 8, 371, 1049, 1212, 1401, 1552
MboI GATC 6 cut(s) 6, 369, 1047, 1210, 1399, 1550
MboII GAAGA 8 cut(s) 350, 663, 1147, 1268, 1591, 1937, 2141, 2144
MfeI CAATTG 3 cut(s) 190, 721, 799
MhlI GDGCHC 3 cut(s) 916, 1387, 1536
MlsI TGGCCA 1 cut(s) 2000
MluNI TGGCCA 1 cut(s) 2000
MlyI GAGTC 1 cut(s) 1305
MmeI TCCRAC 1 cut(s) 591
Mox20I TGGCCA 1 cut(s) 2000
Mph1103I ATGCAT 3 cut(s) 234, 319, 788
MroXI GAANNNNTTC 1 cut(s) 705
MscI TGGCCA 1 cut(s) 2000
MseI TTAA 7 cut(s) 618, 944, 1152, 1410, 2112, 2160, 2264
MslI CAYNNNNRTG 7 cut(s) 237, 603, 819, 882, 1446, 1650, 2277
Msp20I TGGCCA 1 cut(s) 2000
MspA1I CMGCKG 1 cut(s) 80
MspI CCGG 3 cut(s) 1343, 1877, 2220
MspR9I CCNGG 3 cut(s) 855, 954, 1781
MunI CAATTG 3 cut(s) 190, 721, 799
MvaI CCWGG 3 cut(s) 855, 954, 1781
MvnI CGCG 1 cut(s) 1598
MwoI GCNNNNNNNGC 9 cut(s) 83, 89, 286, 352, 492, 641, 788, 1902, 2006
NcoI CCATGG 2 cut(s) 1516, 1920
NdeI CATATG 1 cut(s) 319
NdeII GATC 6 cut(s) 6, 369, 1047, 1210, 1399, 1550
NheI GCTAGC 1 cut(s) 584
NlaIV GGNNCC 3 cut(s) 1384, 1728, 1785
NmeAIII GCCGAG 1 cut(s) 322
NmuCI GTSAC 3 cut(s) 407, 974, 2086
NsiI ATGCAT 3 cut(s) 234, 319, 788
NspI RCATGY 3 cut(s) 786, 1125, 2251
PaeI GCATGC 2 cut(s) 786, 2251
PceI AGGCCT 1 cut(s) 2044
PciI ACATGT 1 cut(s) 1121
PcsI WCGNNNNNNNCGW 1 cut(s) 2234
PdmI GAANNNNTTC 1 cut(s) 705
PfeI GAWTC 2 cut(s) 1028, 1939
PflMI CCANNNNNTGG 1 cut(s) 2276
PfoI TCCNGGA 1 cut(s) 952
PinAI ACCGGT 1 cut(s) 2219
PkrI GCNGC 9 cut(s) 34, 58, 79, 82, 85, 122, 485, 488, 629
Ple19I CGATCG 1 cut(s) 1213
PleI GAGTC 1 cut(s) 1304
PpsI GAGTC 1 cut(s) 1304
PscI ACATGT 1 cut(s) 1121
PshBI ATTAAT 1 cut(s) 2160
Psp6I CCWGG 3 cut(s) 853, 952, 1779
PspGI CCWGG 3 cut(s) 853, 952, 1779
PspN4I GGNNCC 3 cut(s) 1384, 1728, 1785
PspPI GGNCC 2 cut(s) 726, 1783
PstI CTGCAG 1 cut(s) 629
PstNI CAGNNNCTG 2 cut(s) 80, 83
PvuI CGATCG 1 cut(s) 1213
PvuII CAGCTG 1 cut(s) 80
RsaI GTAC 5 cut(s) 64, 568, 875, 1488, 2227
RsaNI GTAC 5 cut(s) 63, 567, 874, 1487, 2226
RseI CAYNNNNRTG 7 cut(s) 237, 603, 819, 882, 1446, 1650, 2277
SaqAI TTAA 7 cut(s) 618, 944, 1152, 1410, 2112, 2160, 2264
SatI GCNGC 9 cut(s) 33, 57, 78, 81, 84, 121, 484, 487, 628
Sau3AI GATC 6 cut(s) 6, 369, 1047, 1210, 1399, 1550
Sau96I GGNCC 2 cut(s) 726, 1783
ScaI AGTACT 1 cut(s) 64
SchI GAGTC 1 cut(s) 1305
ScrFI CCNGG 3 cut(s) 855, 954, 1781
SduI GDGCHC 3 cut(s) 916, 1387, 1536
SfaNI GCATC 7 cut(s) 219, 304, 480, 1026, 1883, 1914, 2006
SfcI CTRYAG 2 cut(s) 625, 828
SinI GGWCC 1 cut(s) 1783
SmiMI CAYNNNNRTG 7 cut(s) 237, 603, 819, 882, 1446, 1650, 2277
SmlI CTYRAG 4 cut(s) 87, 917, 1262, 2020
SmoI CTYRAG 4 cut(s) 87, 917, 1262, 2020
SpeI ACTAGT 1 cut(s) 1495
SphI GCATGC 2 cut(s) 786, 2251
SseBI AGGCCT 1 cut(s) 2044
SsiI CCGC 5 cut(s) 32, 56, 120, 417, 1598
SspI AATATT 1 cut(s) 1669
SspMI CTAG 6 cut(s) 60, 585, 675, 1496, 1850, 2208
StuI AGGCCT 1 cut(s) 2044
StyD4I CCNGG 3 cut(s) 853, 952, 1779
StyI CCWWGG 6 cut(s) 427, 490, 1105, 1300, 1516, 1920
TaaI ACNGT 9 cut(s) 397, 574, 812, 939, 1084, 1324, 1608, 1987, 2262
TaiI ACGT 1 cut(s) 2178
TaqI TCGA 7 cut(s) 5, 325, 1209, 1226, 1373, 1648, 2256
TatI WGTACW 1 cut(s) 62
TauI GCSGC 3 cut(s) 35, 59, 123
TfiI GAWTC 2 cut(s) 1028, 1939
Tru1I TTAA 7 cut(s) 618, 944, 1152, 1410, 2112, 2160, 2264
Tru9I TTAA 7 cut(s) 618, 944, 1152, 1410, 2112, 2160, 2264
TscAI CASTG 8 cut(s) 400, 502, 556, 703, 717, 851, 1611, 2239
TseFI GTSAC 3 cut(s) 407, 974, 2086
TseI GCWGC 6 cut(s) 77, 80, 83, 483, 486, 627
Tsp45I GTSAC 3 cut(s) 407, 974, 2086
TspGWI ACGGA 3 cut(s) 948, 1496, 2091
TspRI CASTG 8 cut(s) 400, 502, 556, 703, 717, 851, 1611, 2239
Van91I CCANNNNNTGG 1 cut(s) 2276
VpaK11BI GGWCC 1 cut(s) 1783
VspI ATTAAT 1 cut(s) 2160
XapI RAATTY 3 cut(s) 179, 1110, 1505
XceI RCATGY 3 cut(s) 786, 1125, 2251
XcmI CCANNNNNNNNNTGG 1 cut(s) 851
XmiI GTMKAC 1 cut(s) 1500
XmnI GAANNNNTTC 1 cut(s) 705
XspI CTAG 6 cut(s) 60, 585, 675, 1496, 1850, 2208
ZrmI AGTACT 1 cut(s) 64
Zsp2I ATGCAT 3 cut(s) 234, 319, 788
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.