FvH4_3g10281

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000066
Physical Location & Seq
Reverse (-)
5996332 .. 5998754
2423 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g10281.t1

Sequence Viewer

Length: 2067 bp
ATGGAACCCCCATCAGCAAACTGGACTGATAGTAATATCCACATCACTAACTTCTGGCTTACAGAGGGTGAGATGCAAATAATGAGTTACACTGCTCGAGATTGTTATGACCAGAAAGGCGAGGGGACCTACCAAAACAGCCCCGAGTTAACGCTGATTCCGCCTTATACCATATCTGCTACCAAAAACAAGTTCATCGCGGTTGGCTGCGACACATACGCAATCTTTACAGGATACCAAGGGGAACAAAGGTACATAACCGAGTGCATGTCCTTGTGTAACAGCCTTGGCAGTGTTGATGAGTCTTGCTCTGGCGTTGGTTGTTGCCAAACCTCCATCCCTAGTGGAATGAAAAACCGTACTGTCACATTGAGTAGCTACTATAATCACTCTGATATATGGAGTTTCAACCCCTGCAGCTACGCATTCATTGTGCAAGAAGGTCATTTTCGTTTCTCTAATACAAGCTTTCAAGAACTGAGTGTCACCGAACAAGTGCCAATGGTTATTGACTGGGCAATTGGGAATGAACCAGACCCATGTGATGCAGCTGAAAGGAGGCAGGATTTTGCCTGCAATAACAATAGCGAATGCGTCAACCACGACAACAGGAATGGTTATGTTTGCGAGTGTTTTCCAGGTAATGAAGGCAATCCATACCTCCCGGACGGTTGCCAAGATATTGATAACTGCAAGGATTCAAACCCCTGCAAGATCGGAACATGTGTAAATTCACCTCCGGGAGATTACTCTTGTTTATGTCCAAAAGGATACAGAAACGACGTCATGGATGAAAAGACCTGCATCAAAGAAAATCCCAAAAACCAATCAAAGATCACTCTCCTGCTTGTTATTTCATTGAGTGTCAGTCTAGGCTTCTTGGTTATACTGGTCGGGATTTCATGGATATATTGGGCGATGAAGAAAAGAGATTTCATCAAACTAAAAGTGAAGTACTTCAGAGAAAATGGAGGTTTACTGTTACAGCAACAACTAGCTAGCCATGGAATGGTGACAGCAAAACTCTATAGTGCAGAAGAACTTGAGAAGGCCACAAACTATTACCATGTAAGTACAGTCCTTGGTAAAGGAAGTTACGGAACAGTATACAAGGGAATACTACCAGATAACAAAGAGGTCGCAGTTAAAAAGTCCAAAACTGGTCCTCGTGCTCAGAGTGATCAGTTTGTTAATGAGGTGATAATTGTTTCTGAAATAAACCATAGAAATGTGGTGAGGTTATTAGGTTGTTGCTTAGAGACAGAAGTGCCTTTACTGGTTTATGAGTTCATCAGCCGTGGAACTCTTTCTGAACACATTCATAAAGAAGTCAAAGGAACATCACTATCATTGGGTTTACGACTGAAGATAGCAGCAGAAACAGCTGAAGCACTAGCATACTTACACTCGTCCACTTCCATGCCAATCATTCACCGAGATGTTAAAGCAACAGATATACTACTCGATGAAAGTTACACAGCGAAAGTGTCAGATTTTGGAGCTTCAAGATTGGTTCCTCTTGATCAAAATCAACTATCAACTTTAGTGCAAGGGACACTGGGATACCTGGACCCTGAATACTTGCTTACGAATCAGCTAACAGAAAAGAGTGATGTCTACAGCTTTGGAGTTGTTCTTGTGGAACTGCTAACTAGTAAAGTTGCACTTTCTTTTGACCGCTGTGAGGAAGAAAGAAGCCTAGCAAGCTTCTTTGTTCGTTCAATACAGGAGGATCGCTTGAAAGAAATCATCGATGATGACATAGTCAATGATGGAAATGTTGATGAGACACTGAAAAATATGGCTAATCTTGCAAAAAGACGTTTGAGATTAAAAGGGGAGGAAAGGCCTACCATGAAAGAAGTAGCCACAGAGCTGGATGGAATGAGAACTAAAATAATGCATCCAAGGGGTAAAGCTGATTTTGGCTCAGAAGAGACTGAGAATTTGGAGACGGGTACTGTTGATGGTAAATGTGACTATGGTTTCAGTAGTACAATTACTAGTACAGCTAGTTGGTATGACAGTATGCAAAAACAAATGTTAATGCCATATGATGATGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK

Protein Analysis

689

Amino Acids

76.91

Weight (kDa)

5.04

Isoelectric Point (pI)

34.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 355 - 623 1.3e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 357 - 626 5.1e-45 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21210 AT1G21230 AT1G21240 AT1G21240 AT1G21245 AT1G21250 AT1G21270 AT1G22720 AT3G25490
fragaria_vesca FvH4_2g35051 FvH4_2g35051 FvH4_2g35060 FvH4_3g10281 FvH4_3g10285 FvH4_3g10286 FvH4_3g10311 FvH4_3g10311 FvH4_3g10320 FvH4_3g10332 FvH4_3g10340 FvH4_3g10350 FvH4_3g10360 FvH4_3g10390 FvH4_3g10412 FvH4_3g10413 FvH4_3g10461 FvH4_3g10462 FvH4_3g10470 FvH4_3g10510 FvH4_3g10520 FvH4_3g10530 FvH4_3g10550 FvH4_3g10560 FvH4_3g10611 FvH4_3g11730 FvH4_6g53140 FvH4_6g53142 FvH4_7g31880 FvH4_7g31910 FvH4_7g31920
malus_domestica MD05G1269900.v1.1 MD05G1270000.v1.1 MD05G1270200.v1.1 MD05G1270400.v1.1 MD05G1270500.v1.1 MD05G1270600.v1.1 MD05G1270800.v1.1 MD05G1270900.v1.1 MD07G1183800.v1.1 MD08G1107600.v1.1 MD10G1248500.v1.1 MD10G1249200.v1.1 MD10G1249500.v1.1 MD10G1250000.v1.1 MD10G1250500.v1.1 MD10G1250900.v1.1 MD10G1251200.v1.1 MD10G1251400.v1.1 MD13G1004600.v1.1 MD15G1088400.v1.1
prunus_persica Prupe.1G442400_v2.0.a1 Prupe.1G442500_v2.0.a1 Prupe.2G223400_v2.0.a1 Prupe.4G091900_v2.0.a1 Prupe.4G093200_v2.0.a1 Prupe.4G093300_v2.0.a1 Prupe.4G093400_v2.0.a1 Prupe.4G093500_v2.0.a1 Prupe.4G094100_v2.0.a1 Prupe.4G094200_v2.0.a1 Prupe.4G094300_v2.0.a1 Prupe.6G358300_v2.0.a1
pyrus_communis pycom08g08990 pycom10g20660 pycom10g20670 pycom10g20680 pycom10g20710 pycom10g20740 pycom13g00400 pycom15g08290
rosa_chinensis RchiOBHm_Chr1g0326861 RchiOBHm_Chr1g0326871 RchiOBHm_Chr1g0331391 RchiOBHm_Chr1g0336911 RchiOBHm_Chr1g0364001 RchiOBHm_Chr2g0174541 RchiOBHm_Chr2g0175041 RchiOBHm_Chr2g0175101 RchiOBHm_Chr2g0175121 RchiOBHm_Chr3g0486331 RchiOBHm_Chr5g0015581 RchiOBHm_Chr5g0016551 RchiOBHm_Chr5g0016561 RchiOBHm_Chr5g0016581 RchiOBHm_Chr5g0016601 RchiOBHm_Chr5g0016611 RchiOBHm_Chr5g0016641 RchiOBHm_Chr5g0016661 RchiOBHm_Chr5g0016691 RchiOBHm_Chr5g0016701 RchiOBHm_Chr5g0016731 RchiOBHm_Chr5g0016741 RchiOBHm_Chr5g0016811 RchiOBHm_Chr5g0016841 RchiOBHm_Chr5g0016871 RchiOBHm_Chr5g0016881 RchiOBHm_Chr5g0016931 RchiOBHm_Chr5g0017021 RchiOBHm_Chr5g0017041 RchiOBHm_Chr5g0017071 RchiOBHm_Chr5g0017091 RchiOBHm_Chr5g0017121 RchiOBHm_Chr5g0017141 RchiOBHm_Chr5g0017291 RchiOBHm_Chr5g0017321 RchiOBHm_Chr5g0017401 RchiOBHm_Chr5g0019001 RchiOBHm_Chr5g0019071 RchiOBHm_Chr5g0028941 RchiOBHm_Chr5g0052141 RchiOBHm_Chr5g0054721 RchiOBHm_Chr5g0054801 RchiOBHm_Chr5g0054931 RchiOBHm_Chr5g0054941 RchiOBHm_Chr6g0259291 RchiOBHm_Chr6g0292991 RchiOBHm_Chr6g0306911
rosa_laevigata RLG00000009465 RLG00000009468 RLG00000010755 RLG00000011976 RLG00000014541 RLG00000014542 RLG00000015169 RLG00000022308 RLG00000027529 RLG00000029324 RLG00000030244 RLG00000030245 RLG00000032210 RLG00000032211 RLG00000032257 RLG00000032259 RLG00000032260 RLG00000032261 RLG00000032262 RLG00000032268 RLG00000032269 RLG00000032272 RLG00000032282 RLG00000032283 RLG00000032308 RLG00000032413 RLG00000033129 RLG00000034945
rosa_multiflora Rmu_co8241929.1_g000001 Rmu_co8269227.1_g000001 Rmu_co8461259.1_g000001 Rmu_co8495385.1_g000001 Rmu_sc0000028.1_g000003 Rmu_sc0000083.1_g000009 Rmu_sc0000083.1_g000010 Rmu_sc0000083.1_g000031 Rmu_sc0000083.1_g000043 Rmu_sc0000083.1_g000046 Rmu_sc0000083.1_g000047 Rmu_sc0000563.1_g000024 Rmu_sc0000842.1_g000012 Rmu_sc0001235.1_g000001 Rmu_sc0001235.1_g000005 Rmu_sc0001235.1_g000009 Rmu_sc0001235.1_g000015 Rmu_sc0001235.1_g000016 Rmu_sc0001235.1_g000017 Rmu_sc0001473.1_g000036 Rmu_sc0001607.1_g000016 Rmu_sc0001788.1_g000006 Rmu_sc0002018.1_g000008 Rmu_sc0002028.1_g000001 Rmu_sc0002680.1_g000011 Rmu_sc0002798.1_g000023 Rmu_sc0002886.1_g000023 Rmu_sc0002989.1_g000012 Rmu_sc0003064.1_g000009 Rmu_sc0003382.1_g000006 Rmu_sc0003630.1_g000002 Rmu_sc0003630.1_g000047 Rmu_sc0003630.1_g000066 Rmu_sc0003630.1_g000067 Rmu_sc0003649.1_g000010 Rmu_sc0003688.1_g000005 Rmu_sc0003920.1_g000007 Rmu_sc0004765.1_g000007 Rmu_sc0004765.1_g000008 Rmu_sc0004920.1_g000011 Rmu_sc0005223.1_g000008 Rmu_sc0005223.1_g000010 Rmu_sc0005223.1_g000015 Rmu_sc0005223.1_g000016 Rmu_sc0005319.1_g000014 Rmu_sc0005319.1_g000015 Rmu_sc0005319.1_g000018 Rmu_sc0005319.1_g000019 Rmu_sc0005319.1_g000025 Rmu_sc0005418.1_g000007 Rmu_sc0005418.1_g000012 Rmu_sc0006106.1_g000011 Rmu_sc0006914.1_g000012 Rmu_sc0007023.1_g000001 Rmu_sc0007662.1_g000001 Rmu_sc0007920.1_g000002 Rmu_sc0007920.1_g000013 Rmu_sc0008053.1_g000016 Rmu_sc0008053.1_g000017 Rmu_sc0008053.1_g000018 Rmu_sc0008053.1_g000023 Rmu_sc0008053.1_g000050 Rmu_sc0009042.1_g000007 Rmu_sc0009042.1_g000008 Rmu_sc0010642.1_g000008 Rmu_sc0011109.1_g000002 Rmu_sc0011314.1_g000006 Rmu_sc0011974.1_g000006 Rmu_sc0012179.1_g000009 Rmu_sc0015169.1_g000001 Rmu_sc0016368.1_g000002 Rmu_sc0022659.1_g000001 Rmu_sc0027371.1_g000001 Rmu_sc0028213.1_g000001 Rmu_sc0034526.1_g000001 Rmu_sc0039366.1_g000002 Rmu_ssc0000050.1_g000084 Rmu_ssc0000050.1_g000088 Rmu_ssc0000167.1_g000017
rosa_roxburghii Rroxscaffold_1G00011340 Rroxscaffold_1G00025710 Rroxscaffold_1G00025870 Rroxscaffold_1G00026070 Rroxscaffold_1G00026170 Rroxscaffold_1G00026200 Rroxscaffold_1G00026270 Rroxscaffold_1G00050950 Rroxscaffold_1G00058900 Rroxscaffold_1G00060040 Rroxscaffold_1G00060060 Rroxscaffold_1G00060070 Rroxscaffold_1G00060080 Rroxscaffold_1G00060110 Rroxscaffold_1G00060120 Rroxscaffold_1G00060140 Rroxscaffold_1G00060170 Rroxscaffold_1G00060210 Rroxscaffold_1G00060220 Rroxscaffold_1G00060240 Rroxscaffold_1G00060250 Rroxscaffold_1G00060260 Rroxscaffold_1G00060300 Rroxscaffold_1G00060320 Rroxscaffold_1G00060330 Rroxscaffold_1G00060900 Rroxscaffold_1G00060920 Rroxscaffold_1G00073980 Rroxscaffold_2G00077560 Rroxscaffold_2G00077580 Rroxscaffold_2G00146990 Rroxscaffold_3G00236200 Rroxscaffold_3G00236210 Rroxscaffold_3G00236220 Rroxscaffold_4G00292760 Rroxscaffold_4G00315200 Rroxscaffold_4G00315240 Rroxscaffold_4G00326240 Rroxscaffold_4G00328980 Rroxscaffold_5G00341890 Rroxscaffold_7G00161350 Rroxscaffold_7G00174490 Rroxscaffold_7G00206550
rosa_rugosa Rorug01G0093000 Rorug01G0093100 Rorug01G0128800 Rorug01G0312000 Rorug01G0312000 Rorug02G0581100 Rorug03G0224600 Rorug05G0028500 Rorug05G0032700 Rorug05G0032800 Rorug05G0032900 Rorug05G0032900 Rorug05G0033000 Rorug05G0033100 Rorug05G0033300 Rorug05G0033400 Rorug05G0033900 Rorug05G0034900 Rorug05G0035000 Rorug05G0046900 Rorug05G0047500 Rorug05G0047500 Rorug05G0111600 Rorug05G0287900 Rorug05G0288700 Rorug05G0586900 Rorug06G0231700 Rorug06G0354300 Rorug06G0354400
rosa_samantha Rh1DG122200 Rh2DG686800 Rh5BG136600 Rh6AG344500 Rh6BG351900 Rh6CG359000 Rh6CG359100 Rh6CG387600 Rh6CG480500 Rh6DG344600 Rh6DG467400
rosa_wichuraiana Rw0G010250 Rw0G014820 Rw0G021400 Rw0G023100 Rw1G009450 Rw1G012100 Rw1G012180 Rw1G028360 Rw2G054260 Rw3G024370 Rw3G024420 Rw3G024470 Rw5G010850 Rw5G011010 Rw5G011020 Rw5G011050 Rw5G011080 Rw5G011120 Rw5G012300 Rw5G018470 Rw5G033830 Rw6G008930 Rw6G030070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 786
Acc36I ACCTGC 1 cut(s) 809
AccB7I CCANNNNNTGG 1 cut(s) 1009
AccI GTMKAC 2 cut(s) 1107, 1617
AccII CGCG 1 cut(s) 200
AciI CCGC 3 cut(s) 161, 200, 1678
AclWI GGATC 1 cut(s) 1740
AcsI RAATTY 2 cut(s) 730, 1945
AcuI CTGAAG 3 cut(s) 943, 1385, 1407
AcyI GRCGYC 1 cut(s) 783
AfaI GTAC 7 cut(s) 254, 361, 956, 1075, 1960, 1996, 2008
AfiI CCNNNNNNNGG 2 cut(s) 1009, 1684
AflIII ACRYGT 1 cut(s) 722
AgsI TTSAA 6 cut(s) 409, 473, 702, 1506, 1722, 1741
AhlI ACTAGT 2 cut(s) 1652, 2003
AjnI CCWGG 2 cut(s) 637, 1566
AjuI GAANNNNNNNTTGG 2 cut(s) 176, 208
Alw21I GWGCWC 1 cut(s) 1174
Alw26I GTCTC 4 cut(s) 1253, 1781, 1931, 1946
AlwI GGATC 1 cut(s) 1740
Ama87I CYCGRG 2 cut(s) 96, 143
AoxI GGCC 2 cut(s) 1050, 1847
ApeKI GCWGC 4 cut(s) 207, 417, 548, 1373
ApoI RAATTY 2 cut(s) 730, 1945
ArsI GACNNNNNNTTYG 2 cut(s) 1930, 1962
Asp700I GAANNNNTTC 3 cut(s) 956, 1306, 1317
AspS9I GGNCC 3 cut(s) 126, 1163, 1570
AsuC2I CCSGG 2 cut(s) 665, 741
AsuHPI GGTGA 7 cut(s) 80, 478, 726, 1024, 1210, 1246, 1424
AsuNHI GCTAGC 1 cut(s) 998
AvaI CYCGRG 2 cut(s) 96, 143
AvaII GGWCC 3 cut(s) 126, 1163, 1570
BarI GAAGNNNNNNTAC 2 cut(s) 1257, 1289
BauI CACGAG 1 cut(s) 1167
Bbv12I GWGCWC 1 cut(s) 1174
BbvI GCAGC 4 cut(s) 194, 429, 560, 1385
BccI CCATC 6 cut(s) 19, 344, 1766, 1874, 1961, 2054
BceAI ACGGC 1 cut(s) 1281
BciT130I CCWGG 2 cut(s) 639, 1568
BciVI GTATCC 3 cut(s) 227, 764, 1556
BclI TGATCA 2 cut(s) 1180, 1522
BcnI CCSGG 2 cut(s) 665, 741
BcoDI GTCTC 4 cut(s) 1253, 1781, 1931, 1946
BcuI ACTAGT 2 cut(s) 1652, 2003
BfaI CTAG 9 cut(s) 342, 872, 995, 999, 1394, 1653, 1700, 2004, 2013
BfmI CTRYAG 3 cut(s) 415, 1027, 1618
BfuAI ACCTGC 1 cut(s) 809
BfuI GTATCC 3 cut(s) 227, 764, 1556
BisI GCNGC 4 cut(s) 208, 418, 549, 1374
BlsI GCNGC 4 cut(s) 209, 419, 550, 1375
BmcAI AGTACT 1 cut(s) 956
Bme1390I CCNGG 4 cut(s) 639, 665, 741, 1568
Bme18I GGWCC 3 cut(s) 126, 1163, 1570
BmeT110I CYCGRG 2 cut(s) 96, 143
BmgT120I GGNCC 3 cut(s) 126, 1163, 1570
BmiI GGNNCC 4 cut(s) 6, 127, 1515, 1572
BmrFI CCNGG 4 cut(s) 639, 665, 741, 1568
BmrI ACTGGG 2 cut(s) 523, 1568
BmsI GCATC 4 cut(s) 63, 535, 813, 1912
BmtI GCTAGC 1 cut(s) 1002
BmuI ACTGGG 2 cut(s) 523, 1568
BplI GAGNNNNNCTC 2 cut(s) 293, 325
BpuEI CTTGAG 1 cut(s) 1064
BpuMI CCSGG 2 cut(s) 665, 741
Bsa29I ATCGAT 1 cut(s) 1752
BsaBI GATNNNNATC 1 cut(s) 1527
BsaHI GRCGYC 1 cut(s) 783
BsaJI CCNNGG 6 cut(s) 238, 286, 1003, 1081, 1297, 1907
Bsc4I CCNNNNNNNGG 2 cut(s) 1009, 1684
Bse1I ACTGG 6 cut(s) 26, 518, 894, 1165, 1281, 1563
Bse8I GATNNNNATC 1 cut(s) 1527
BseBI CCWGG 2 cut(s) 639, 1568
BseCI ATCGAT 1 cut(s) 1752
BseDI CCNNGG 6 cut(s) 238, 286, 1003, 1081, 1297, 1907
BseGI GGATG 4 cut(s) 336, 796, 1885, 1903
BseJI GATNNNNATC 1 cut(s) 1527
BseLI CCNNNNNNNGG 2 cut(s) 1009, 1684
BseMII CTCAG 4 cut(s) 470, 1187, 1932, 1944
BseNI ACTGG 6 cut(s) 26, 518, 894, 1165, 1281, 1563
BseXI GCAGC 4 cut(s) 194, 429, 560, 1385
BsgI GTGCAG 1 cut(s) 1053
Bsh1236I CGCG 1 cut(s) 200
BshFI GGCC 2 cut(s) 1052, 1849
BshVI ATCGAT 1 cut(s) 1752
BsiHKAI GWGCWC 1 cut(s) 1174
BsiHKCI CYCGRG 2 cut(s) 96, 143
BsiSI CCGG 2 cut(s) 665, 740
BslFI GGGAC 2 cut(s) 139, 1567
BslI CCNNNNNNNGG 2 cut(s) 1009, 1684
BsmAI GTCTC 4 cut(s) 1253, 1781, 1931, 1946
BsmBI CGTCTC 1 cut(s) 1946
BsmFI GGGAC 2 cut(s) 139, 1567
BsmI GAATGC 2 cut(s) 425, 596
BsnI GGCC 2 cut(s) 1052, 1849
BsoBI CYCGRG 2 cut(s) 96, 143
Bsp1286I GDGCHC 1 cut(s) 1174
Bsp143I GATC 5 cut(s) 714, 834, 1180, 1522, 1732
Bsp19I CCATGG 1 cut(s) 1003
BspACI CCGC 3 cut(s) 161, 200, 1678
BspANI GGCC 2 cut(s) 1052, 1849
BspCNI CTCAG 4 cut(s) 471, 1186, 1933, 1943
BspDI ATCGAT 1 cut(s) 1752
BspFNI CGCG 1 cut(s) 200
BspLI GGNNCC 4 cut(s) 6, 127, 1515, 1572
BspMAI CTGCAG 1 cut(s) 419
BspMI ACCTGC 1 cut(s) 809
BspOI GCTAGC 1 cut(s) 1002
BspPI GGATC 1 cut(s) 1740
BsrI ACTGG 6 cut(s) 26, 518, 894, 1165, 1281, 1563
BssECI CCNNGG 6 cut(s) 238, 286, 1003, 1081, 1297, 1907
BssMI GATC 5 cut(s) 714, 834, 1180, 1522, 1732
BssNAI GTATAC 1 cut(s) 1108
BssNI GRCGYC 1 cut(s) 783
BssSI CACGAG 1 cut(s) 1167
BssT1I CCWWGG 5 cut(s) 238, 286, 1003, 1081, 1907
Bst1107I GTATAC 1 cut(s) 1108
Bst2BI CACGAG 1 cut(s) 1167
Bst2UI CCWGG 2 cut(s) 639, 1568
Bst4CI ACNGT 8 cut(s) 359, 364, 671, 981, 1078, 1105, 1963, 2027
Bst6I CTCTTC 1 cut(s) 1929
BstACI GRCGYC 1 cut(s) 783
BstC8I GCNNGC 3 cut(s) 574, 1000, 1705
BstDEI CTNAG 5 cut(s) 479, 1173, 1255, 1930, 1941
BstDSI CCRYGG 2 cut(s) 1003, 1297
BstF5I GGATG 4 cut(s) 336, 796, 1885, 1903
BstFNI CGCG 1 cut(s) 200
BstKTI GATC 5 cut(s) 717, 837, 1183, 1525, 1735
BstMAI GTCTC 4 cut(s) 1253, 1781, 1931, 1946
BstMBI GATC 5 cut(s) 714, 834, 1180, 1522, 1732
BstMWI GCNNNNNNNGC 4 cut(s) 160, 1382, 1704, 1811
BstNI CCWGG 2 cut(s) 639, 1568
BstNSI RCATGY 2 cut(s) 271, 726
BstSCI CCNGG 4 cut(s) 637, 663, 739, 1566
BstSFI CTRYAG 3 cut(s) 415, 1027, 1618
BstUI CGCG 1 cut(s) 200
BstV1I GCAGC 4 cut(s) 194, 429, 560, 1385
BstXI CCANNNNNNTGG 1 cut(s) 1876
BstZ17I GTATAC 1 cut(s) 1108
Bsu15I ATCGAT 1 cut(s) 1752
BsuI GTATCC 3 cut(s) 227, 764, 1556
BsuRI GGCC 2 cut(s) 1052, 1849
BsuTUI ATCGAT 1 cut(s) 1752
BtgI CCRYGG 2 cut(s) 1003, 1297
BtgZI GCGATG 2 cut(s) 181, 932
BtsCI GGATG 4 cut(s) 336, 796, 1885, 1903
BtsI GCAGTG 2 cut(s) 90, 298
BtsIMutI CAGTG 4 cut(s) 90, 298, 1556, 1790
BveI ACCTGC 1 cut(s) 809
Cac8I GCNNGC 3 cut(s) 574, 1000, 1705
Cfr13I GGNCC 3 cut(s) 126, 1163, 1570
ClaI ATCGAT 1 cut(s) 1752
CseI GACGC 1 cut(s) 583
Csp6I GTAC 7 cut(s) 253, 360, 955, 1074, 1959, 1995, 2007
CviAII CATG 9 cut(s) 268, 540, 723, 787, 903, 1004, 1067, 1420, 1855
CviQI GTAC 7 cut(s) 253, 360, 955, 1074, 1959, 1995, 2007
DdeI CTNAG 5 cut(s) 479, 1173, 1255, 1930, 1941
DpnI GATC 5 cut(s) 716, 836, 1182, 1524, 1734
DpnII GATC 5 cut(s) 714, 834, 1180, 1522, 1732
Eam1104I CTCTTC 1 cut(s) 1929
EarI CTCTTC 1 cut(s) 1929
EciI GGCGGA 1 cut(s) 150
Eco130I CCWWGG 5 cut(s) 238, 286, 1003, 1081, 1907
Eco147I AGGCCT 1 cut(s) 1849
Eco47I GGWCC 3 cut(s) 126, 1163, 1570
Eco57I CTGAAG 3 cut(s) 943, 1385, 1407
Eco88I CYCGRG 2 cut(s) 96, 143
EcoO109I RGGNCCY 1 cut(s) 126
EcoRII CCWGG 2 cut(s) 637, 1566
EcoT14I CCWWGG 5 cut(s) 238, 286, 1003, 1081, 1907
EcoT22I ATGCAT 1 cut(s) 1905
ErhI CCWWGG 5 cut(s) 238, 286, 1003, 1081, 1907
Esp3I CGTCTC 1 cut(s) 1946
FaeI CATG 9 cut(s) 271, 543, 726, 790, 906, 1007, 1070, 1423, 1858
FalI AAGNNNNNCTT 2 cut(s) 1650, 1682
FaqI GGGAC 2 cut(s) 139, 1567
FatI CATG 9 cut(s) 267, 539, 722, 786, 902, 1003, 1066, 1419, 1854
FauNDI CATATG 1 cut(s) 2053
FbaI TGATCA 2 cut(s) 1180, 1522
FblI GTMKAC 2 cut(s) 1107, 1617
Fnu4HI GCNGC 4 cut(s) 208, 418, 549, 1374
FokI GGATG 4 cut(s) 323, 803, 1890, 1892
Fsp4HI GCNGC 4 cut(s) 208, 418, 549, 1374
FspBI CTAG 9 cut(s) 342, 872, 995, 999, 1394, 1653, 1700, 2004, 2013
GluI GCNGC 4 cut(s) 208, 418, 549, 1374
HaeIII GGCC 2 cut(s) 1052, 1849
HapII CCGG 2 cut(s) 665, 740
HgaI GACGC 1 cut(s) 583
Hin1I GRCGYC 1 cut(s) 783
Hin1II CATG 9 cut(s) 271, 543, 726, 790, 906, 1007, 1070, 1423, 1858
HincII GTYRAC 2 cut(s) 150, 598
HindII GTYRAC 2 cut(s) 150, 598
HindIII AAGCTT 2 cut(s) 466, 1705
HinfI GANTC 4 cut(s) 157, 302, 698, 1591
HpaI GTTAAC 1 cut(s) 150
HpaII CCGG 2 cut(s) 665, 740
HphI GGTGA 7 cut(s) 80, 478, 726, 1024, 1210, 1246, 1424
Hpy166II GTNNAC 7 cut(s) 150, 598, 977, 1108, 1358, 1413, 1618
Hpy188I TCNGA 8 cut(s) 394, 719, 962, 1176, 1213, 1312, 1492, 1933
Hpy188III TCNNGA 5 cut(s) 98, 473, 895, 1506, 1520
Hpy8I GTNNAC 7 cut(s) 150, 598, 977, 1108, 1358, 1413, 1618
Hpy99I CGWCG 1 cut(s) 785
HpyAV CCTTC 3 cut(s) 434, 641, 1042
HpyCH4III ACNGT 8 cut(s) 359, 364, 671, 981, 1078, 1105, 1963, 2027
HpyCH4IV ACGT 2 cut(s) 783, 1822
HpyF10VI GCNNNNNNNGC 4 cut(s) 160, 1382, 1704, 1811
HpyF3I CTNAG 5 cut(s) 479, 1173, 1255, 1930, 1941
HpySE526I ACGT 2 cut(s) 783, 1822
Hsp92I GRCGYC 1 cut(s) 783
Hsp92II CATG 9 cut(s) 271, 543, 726, 790, 906, 1007, 1070, 1423, 1858
Ksp22I TGATCA 2 cut(s) 1180, 1522
KspAI GTTAAC 1 cut(s) 150
Kzo9I GATC 5 cut(s) 714, 834, 1180, 1522, 1732
LmnI GCTCC 1 cut(s) 1499
Lsp1109I GCAGC 4 cut(s) 194, 429, 560, 1385
LweI GCATC 4 cut(s) 63, 535, 813, 1912
MaeI CTAG 9 cut(s) 342, 872, 995, 999, 1394, 1653, 1700, 2004, 2013
MaeII ACGT 2 cut(s) 783, 1822
MaeIII GTNAC 9 cut(s) 86, 278, 364, 484, 981, 1012, 1094, 1472, 1976
MalI GATC 5 cut(s) 716, 836, 1182, 1524, 1734
MboI GATC 5 cut(s) 714, 834, 1180, 1522, 1732
MboII GAAGA 5 cut(s) 934, 1049, 1378, 1700, 1946
MfeI CAATTG 1 cut(s) 519
MhlI GDGCHC 1 cut(s) 1174
MluCI AATT 5 cut(s) 519, 730, 1203, 1945, 1998
MlyI GAGTC 1 cut(s) 311
Mph1103I ATGCAT 1 cut(s) 1905
MroXI GAANNNNTTC 3 cut(s) 956, 1306, 1317
MseI TTAA 6 cut(s) 149, 1146, 1191, 1443, 1832, 2045
MslI CAYNNNNRTG 3 cut(s) 1227, 1418, 1437
MspA1I CMGCKG 3 cut(s) 551, 1385, 1680
MspI CCGG 2 cut(s) 665, 740
MspR9I CCNGG 4 cut(s) 639, 665, 741, 1568
MunI CAATTG 1 cut(s) 519
Mva1269I GAATGC 2 cut(s) 425, 596
MvaI CCWGG 2 cut(s) 639, 1568
MvnI CGCG 1 cut(s) 200
MwoI GCNNNNNNNGC 4 cut(s) 160, 1382, 1704, 1811
NciI CCSGG 2 cut(s) 665, 741
NcoI CCATGG 1 cut(s) 1003
NdeI CATATG 1 cut(s) 2053
NdeII GATC 5 cut(s) 714, 834, 1180, 1522, 1732
NheI GCTAGC 1 cut(s) 998
NlaIII CATG 9 cut(s) 271, 543, 726, 790, 906, 1007, 1070, 1423, 1858
NlaIV GGNNCC 4 cut(s) 6, 127, 1515, 1572
NmuCI GTSAC 4 cut(s) 364, 484, 1012, 1976
NsiI ATGCAT 1 cut(s) 1905
NspI RCATGY 2 cut(s) 271, 726
PaeR7I CTCGAG 1 cut(s) 96
PceI AGGCCT 1 cut(s) 1849
PciI ACATGT 1 cut(s) 722
PctI GAATGC 2 cut(s) 425, 596
PdmI GAANNNNTTC 3 cut(s) 956, 1306, 1317
PfeI GAWTC 3 cut(s) 157, 698, 1591
PflFI GACNNNGTC 1 cut(s) 1763
PflMI CCANNNNNTGG 1 cut(s) 1009
PfoI TCCNGGA 2 cut(s) 663, 739
PkrI GCNGC 4 cut(s) 209, 419, 550, 1375
PleI GAGTC 1 cut(s) 310
PpsI GAGTC 1 cut(s) 310
PpuMI RGGWCCY 1 cut(s) 126
PscI ACATGT 1 cut(s) 722
Psp5II RGGWCCY 1 cut(s) 126
Psp6I CCWGG 2 cut(s) 637, 1566
PspGI CCWGG 2 cut(s) 637, 1566
PspN4I GGNNCC 4 cut(s) 6, 127, 1515, 1572
PspPI GGNCC 3 cut(s) 126, 1163, 1570
PspPPI RGGWCCY 1 cut(s) 126
PstI CTGCAG 1 cut(s) 419
PsyI GACNNNGTC 1 cut(s) 1763
PvuII CAGCTG 2 cut(s) 551, 1385
RsaI GTAC 7 cut(s) 254, 361, 956, 1075, 1960, 1996, 2008
RsaNI GTAC 7 cut(s) 253, 360, 955, 1074, 1959, 1995, 2007
RseI CAYNNNNRTG 3 cut(s) 1227, 1418, 1437
SaqAI TTAA 6 cut(s) 149, 1146, 1191, 1443, 1832, 2045
SatI GCNGC 4 cut(s) 208, 418, 549, 1374
Sau3AI GATC 5 cut(s) 714, 834, 1180, 1522, 1732
Sau96I GGNCC 3 cut(s) 126, 1163, 1570
ScaI AGTACT 1 cut(s) 956
SchI GAGTC 1 cut(s) 311
ScrFI CCNGG 4 cut(s) 639, 665, 741, 1568
SduI GDGCHC 1 cut(s) 1174
SfaNI GCATC 4 cut(s) 63, 535, 813, 1912
SfcI CTRYAG 3 cut(s) 415, 1027, 1618
Sfr274I CTCGAG 1 cut(s) 96
SinI GGWCC 3 cut(s) 126, 1163, 1570
SlaI CTCGAG 1 cut(s) 96
SmiMI CAYNNNNRTG 3 cut(s) 1227, 1418, 1437
SmlI CTYRAG 2 cut(s) 96, 1043
SmoI CTYRAG 2 cut(s) 96, 1043
SpeI ACTAGT 2 cut(s) 1652, 2003
Sse9I AATT 5 cut(s) 519, 730, 1203, 1945, 1998
SseBI AGGCCT 1 cut(s) 1849
SsiI CCGC 3 cut(s) 161, 200, 1678
SspMI CTAG 9 cut(s) 342, 872, 995, 999, 1394, 1653, 1700, 2004, 2013
StuI AGGCCT 1 cut(s) 1849
StyD4I CCNGG 4 cut(s) 637, 663, 739, 1566
StyI CCWWGG 5 cut(s) 238, 286, 1003, 1081, 1907
TaaI ACNGT 8 cut(s) 359, 364, 671, 981, 1078, 1105, 1963, 2027
TaiI ACGT 2 cut(s) 786, 1825
TaqI TCGA 3 cut(s) 97, 1464, 1752
TasI AATT 5 cut(s) 519, 730, 1203, 1945, 1998
TatI WGTACW 4 cut(s) 954, 1073, 1994, 2006
TfiI GAWTC 3 cut(s) 157, 698, 1591
Tru1I TTAA 6 cut(s) 149, 1146, 1191, 1443, 1832, 2045
Tru9I TTAA 6 cut(s) 149, 1146, 1191, 1443, 1832, 2045
TscAI CASTG 4 cut(s) 97, 298, 1563, 1797
TseFI GTSAC 4 cut(s) 364, 484, 1012, 1976
TseI GCWGC 4 cut(s) 207, 417, 548, 1373
Tsp45I GTSAC 4 cut(s) 364, 484, 1012, 1976
TspGWI ACGGA 1 cut(s) 1113
TspRI CASTG 4 cut(s) 97, 298, 1563, 1797
Tth111I GACNNNGTC 1 cut(s) 1763
Van91I CCANNNNNTGG 1 cut(s) 1009
VpaK11BI GGWCC 3 cut(s) 126, 1163, 1570
XapI RAATTY 2 cut(s) 730, 1945
XceI RCATGY 2 cut(s) 271, 726
XcmI CCANNNNNNNNNTGG 1 cut(s) 18
XhoI CTCGAG 1 cut(s) 96
XmiI GTMKAC 2 cut(s) 1107, 1617
XmnI GAANNNNTTC 3 cut(s) 956, 1306, 1317
XspI CTAG 9 cut(s) 342, 872, 995, 999, 1394, 1653, 1700, 2004, 2013
ZraI GACGTC 1 cut(s) 784
ZrmI AGTACT 1 cut(s) 956
Zsp2I ATGCAT 1 cut(s) 1905
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.