Rw0G010250

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00425
OG0000066
Physical Location & Seq
Forward (+)
13941 .. 15643
1703 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G010250.1

Sequence Viewer

Length: 1521 bp
ATGCAGCCAGATCAAGAACAATGCATGTCAGGGGAACAGCAGTGTTTGGATGTCAAGAATGGGCTCGGGTATCGCTGCAAGTGCAACGAAGGTTACGAAGGGAATCCATACCTAAATGGATGCACTGACATTAATGAATGCGAGGATCCGGTGATTTCCAACAAGTGCAAACAAAGATGCATAAATACAGAGGGGAACTATACGTGTGCTTGCCACAAGGGGTACCATAACAATGGCACAACAGACTCGGTAGATTGCATCGCTGACCACCAAACAATATTTGTTCAAATTATACTTGGGCTCGGTGTTGGCCTCATATCCTTATTAATAGGCATCTCTTGGTTGTACTTGGTCTACAAAAGATGGAAGCTCATGAAGCTCAAGGAGAAGTTCTTCATCCAAAATGGAGGATTGATGTTAAAGCAACAGCTTTCGGAACGACAAGGTGGTGCCTCTAACCAAACGGCCAAAATCTTCACCGAATACCAACTTAAGAAGGCTACCAACCATTTCAGTGAAGCCAGAATCGTTGGCAAAGGAGGTTTCGGTACAGTCTACAAAGGGATTATAGCAGATGAAAGAGCTAAGGAAAAAGTGGTTGCAATCAAGAAATCCAAACTTGTGGATCGAAGCCAAATAGAGCAATTCATTAATGAAGTAGTGGTGCTTTCCCAAATTAATCACAGGAATGTGGTGAAGCTTTTGGGTTGCTGTTTCGAGACAGAAGTCCCACTACTGGTTTATGAGTTTGTCACCAATGGTACGCTATTCGATTACATCCACAACAAAAGCAAGGAATCCAACTTTGCTTGGGAAATTCGTTTAAGGATAGCTGCAGAAACTGCTGGAGTGCTATCACACTTGCACTCTGAAGCTTCTATTCCCATCATTCACAGAGATGTCAAGTCCACAAACATACTCTTGGATGATAATCTTACAGCAAAAGTGTCTGATTTCGGTGCCTCCAGATTGGTTCCGTCAGATCAAGCTCAGCTCTCTACAGTGGTGCAAGGAACGGTAGGGTATTTGGACCCTGAATACTTACAGACGAGCCAGTTGACTGACAAGAGTGATGTCTATAGCTTTGGGGTTGTTCTTGTGGAACTACTAACAGGAAAGAAGGCGCTTTCGGTGGACAAGCCGGAAAAGGAGAGAAATCTAGCCATGTATTTTCTTTCTGCCCTAAGAGAGGACCGATTGGTAGAGGTGATTGATGGTAGTGTCATTAATGGAGCAAACGTTGAGCAGCTTAAGGAAGTTTCAAACCTTGCAAAAAGGTGTTTGAAAGTAGAAGGAAAAGAAAGACCAACCATGAAGGAAGTGGCAATGGAGCTTGAGGGACTGAGAAGGATGGTAATGCATCCATGGGTTAGTAATGATGAATCCAATGCAGAGGAGAATGAACACTTGCTTCATGAAATAACACTGGAAACTTTTAGTCATGGAGGTGGTGGGGATACGAGTTCTGGGTATGATACCATGAGGAACCTGATCACATTACCAGTTGATGATGGGAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF_CA GUB_WAK_bind Pkinase WAK

Protein Analysis

506

Amino Acids

56.56

Weight (kDa)

5.8

Isoelectric Point (pI)

30.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EGF_CA PF07645 43 - 80 2e-06 Calcium-binding EGF domain
Pkinase PF00069 172 - 442 3.8e-49 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 175 - 445 7.9e-50 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21210 AT1G21230 AT1G21240 AT1G21240 AT1G21245 AT1G21250 AT1G21270 AT1G22720 AT3G25490
fragaria_vesca FvH4_2g35051 FvH4_2g35051 FvH4_2g35060 FvH4_3g10281 FvH4_3g10285 FvH4_3g10286 FvH4_3g10311 FvH4_3g10311 FvH4_3g10320 FvH4_3g10332 FvH4_3g10340 FvH4_3g10350 FvH4_3g10360 FvH4_3g10390 FvH4_3g10412 FvH4_3g10413 FvH4_3g10461 FvH4_3g10462 FvH4_3g10470 FvH4_3g10510 FvH4_3g10520 FvH4_3g10530 FvH4_3g10550 FvH4_3g10560 FvH4_3g10611 FvH4_3g11730 FvH4_6g53140 FvH4_6g53142 FvH4_7g31880 FvH4_7g31910 FvH4_7g31920
malus_domestica MD05G1269900.v1.1 MD05G1270000.v1.1 MD05G1270200.v1.1 MD05G1270400.v1.1 MD05G1270500.v1.1 MD05G1270600.v1.1 MD05G1270800.v1.1 MD05G1270900.v1.1 MD07G1183800.v1.1 MD08G1107600.v1.1 MD10G1248500.v1.1 MD10G1249200.v1.1 MD10G1249500.v1.1 MD10G1250000.v1.1 MD10G1250500.v1.1 MD10G1250900.v1.1 MD10G1251200.v1.1 MD10G1251400.v1.1 MD13G1004600.v1.1 MD15G1088400.v1.1
prunus_persica Prupe.1G442400_v2.0.a1 Prupe.1G442500_v2.0.a1 Prupe.2G223400_v2.0.a1 Prupe.4G091900_v2.0.a1 Prupe.4G093200_v2.0.a1 Prupe.4G093300_v2.0.a1 Prupe.4G093400_v2.0.a1 Prupe.4G093500_v2.0.a1 Prupe.4G094100_v2.0.a1 Prupe.4G094200_v2.0.a1 Prupe.4G094300_v2.0.a1 Prupe.6G358300_v2.0.a1
pyrus_communis pycom08g08990 pycom10g20660 pycom10g20670 pycom10g20680 pycom10g20710 pycom10g20740 pycom13g00400 pycom15g08290
rosa_chinensis RchiOBHm_Chr1g0326861 RchiOBHm_Chr1g0326871 RchiOBHm_Chr1g0331391 RchiOBHm_Chr1g0336911 RchiOBHm_Chr1g0364001 RchiOBHm_Chr2g0174541 RchiOBHm_Chr2g0175041 RchiOBHm_Chr2g0175101 RchiOBHm_Chr2g0175121 RchiOBHm_Chr3g0486331 RchiOBHm_Chr5g0015581 RchiOBHm_Chr5g0016551 RchiOBHm_Chr5g0016561 RchiOBHm_Chr5g0016581 RchiOBHm_Chr5g0016601 RchiOBHm_Chr5g0016611 RchiOBHm_Chr5g0016641 RchiOBHm_Chr5g0016661 RchiOBHm_Chr5g0016691 RchiOBHm_Chr5g0016701 RchiOBHm_Chr5g0016731 RchiOBHm_Chr5g0016741 RchiOBHm_Chr5g0016811 RchiOBHm_Chr5g0016841 RchiOBHm_Chr5g0016871 RchiOBHm_Chr5g0016881 RchiOBHm_Chr5g0016931 RchiOBHm_Chr5g0017021 RchiOBHm_Chr5g0017041 RchiOBHm_Chr5g0017071 RchiOBHm_Chr5g0017091 RchiOBHm_Chr5g0017121 RchiOBHm_Chr5g0017141 RchiOBHm_Chr5g0017291 RchiOBHm_Chr5g0017321 RchiOBHm_Chr5g0017401 RchiOBHm_Chr5g0019001 RchiOBHm_Chr5g0019071 RchiOBHm_Chr5g0028941 RchiOBHm_Chr5g0052141 RchiOBHm_Chr5g0054721 RchiOBHm_Chr5g0054801 RchiOBHm_Chr5g0054931 RchiOBHm_Chr5g0054941 RchiOBHm_Chr6g0259291 RchiOBHm_Chr6g0292991 RchiOBHm_Chr6g0306911
rosa_laevigata RLG00000009465 RLG00000009468 RLG00000010755 RLG00000011976 RLG00000014541 RLG00000014542 RLG00000015169 RLG00000022308 RLG00000027529 RLG00000029324 RLG00000030244 RLG00000030245 RLG00000032210 RLG00000032211 RLG00000032257 RLG00000032259 RLG00000032260 RLG00000032261 RLG00000032262 RLG00000032268 RLG00000032269 RLG00000032272 RLG00000032282 RLG00000032283 RLG00000032308 RLG00000032413 RLG00000033129 RLG00000034945
rosa_multiflora Rmu_co8241929.1_g000001 Rmu_co8269227.1_g000001 Rmu_co8461259.1_g000001 Rmu_co8495385.1_g000001 Rmu_sc0000028.1_g000003 Rmu_sc0000083.1_g000009 Rmu_sc0000083.1_g000010 Rmu_sc0000083.1_g000031 Rmu_sc0000083.1_g000043 Rmu_sc0000083.1_g000046 Rmu_sc0000083.1_g000047 Rmu_sc0000563.1_g000024 Rmu_sc0000842.1_g000012 Rmu_sc0001235.1_g000001 Rmu_sc0001235.1_g000005 Rmu_sc0001235.1_g000009 Rmu_sc0001235.1_g000015 Rmu_sc0001235.1_g000016 Rmu_sc0001235.1_g000017 Rmu_sc0001473.1_g000036 Rmu_sc0001607.1_g000016 Rmu_sc0001788.1_g000006 Rmu_sc0002018.1_g000008 Rmu_sc0002028.1_g000001 Rmu_sc0002680.1_g000011 Rmu_sc0002798.1_g000023 Rmu_sc0002886.1_g000023 Rmu_sc0002989.1_g000012 Rmu_sc0003064.1_g000009 Rmu_sc0003382.1_g000006 Rmu_sc0003630.1_g000002 Rmu_sc0003630.1_g000047 Rmu_sc0003630.1_g000066 Rmu_sc0003630.1_g000067 Rmu_sc0003649.1_g000010 Rmu_sc0003688.1_g000005 Rmu_sc0003920.1_g000007 Rmu_sc0004765.1_g000007 Rmu_sc0004765.1_g000008 Rmu_sc0004920.1_g000011 Rmu_sc0005223.1_g000008 Rmu_sc0005223.1_g000010 Rmu_sc0005223.1_g000015 Rmu_sc0005223.1_g000016 Rmu_sc0005319.1_g000014 Rmu_sc0005319.1_g000015 Rmu_sc0005319.1_g000018 Rmu_sc0005319.1_g000019 Rmu_sc0005319.1_g000025 Rmu_sc0005418.1_g000007 Rmu_sc0005418.1_g000012 Rmu_sc0006106.1_g000011 Rmu_sc0006914.1_g000012 Rmu_sc0007023.1_g000001 Rmu_sc0007662.1_g000001 Rmu_sc0007920.1_g000002 Rmu_sc0007920.1_g000013 Rmu_sc0008053.1_g000016 Rmu_sc0008053.1_g000017 Rmu_sc0008053.1_g000018 Rmu_sc0008053.1_g000023 Rmu_sc0008053.1_g000050 Rmu_sc0009042.1_g000007 Rmu_sc0009042.1_g000008 Rmu_sc0010642.1_g000008 Rmu_sc0011109.1_g000002 Rmu_sc0011314.1_g000006 Rmu_sc0011974.1_g000006 Rmu_sc0012179.1_g000009 Rmu_sc0015169.1_g000001 Rmu_sc0016368.1_g000002 Rmu_sc0022659.1_g000001 Rmu_sc0027371.1_g000001 Rmu_sc0028213.1_g000001 Rmu_sc0034526.1_g000001 Rmu_sc0039366.1_g000002 Rmu_ssc0000050.1_g000084 Rmu_ssc0000050.1_g000088 Rmu_ssc0000167.1_g000017
rosa_roxburghii Rroxscaffold_1G00011340 Rroxscaffold_1G00025710 Rroxscaffold_1G00025870 Rroxscaffold_1G00026070 Rroxscaffold_1G00026170 Rroxscaffold_1G00026200 Rroxscaffold_1G00026270 Rroxscaffold_1G00050950 Rroxscaffold_1G00058900 Rroxscaffold_1G00060040 Rroxscaffold_1G00060060 Rroxscaffold_1G00060070 Rroxscaffold_1G00060080 Rroxscaffold_1G00060110 Rroxscaffold_1G00060120 Rroxscaffold_1G00060140 Rroxscaffold_1G00060170 Rroxscaffold_1G00060210 Rroxscaffold_1G00060220 Rroxscaffold_1G00060240 Rroxscaffold_1G00060250 Rroxscaffold_1G00060260 Rroxscaffold_1G00060300 Rroxscaffold_1G00060320 Rroxscaffold_1G00060330 Rroxscaffold_1G00060900 Rroxscaffold_1G00060920 Rroxscaffold_1G00073980 Rroxscaffold_2G00077560 Rroxscaffold_2G00077580 Rroxscaffold_2G00146990 Rroxscaffold_3G00236200 Rroxscaffold_3G00236210 Rroxscaffold_3G00236220 Rroxscaffold_4G00292760 Rroxscaffold_4G00315200 Rroxscaffold_4G00315240 Rroxscaffold_4G00326240 Rroxscaffold_4G00328980 Rroxscaffold_5G00341890 Rroxscaffold_7G00161350 Rroxscaffold_7G00174490 Rroxscaffold_7G00206550
rosa_rugosa Rorug01G0093000 Rorug01G0093100 Rorug01G0128800 Rorug01G0312000 Rorug01G0312000 Rorug02G0581100 Rorug03G0224600 Rorug05G0028500 Rorug05G0032700 Rorug05G0032800 Rorug05G0032900 Rorug05G0032900 Rorug05G0033000 Rorug05G0033100 Rorug05G0033300 Rorug05G0033400 Rorug05G0033900 Rorug05G0034900 Rorug05G0035000 Rorug05G0046900 Rorug05G0047500 Rorug05G0047500 Rorug05G0111600 Rorug05G0287900 Rorug05G0288700 Rorug05G0586900 Rorug06G0231700 Rorug06G0354300 Rorug06G0354400
rosa_samantha Rh1DG122200 Rh2DG686800 Rh5BG136600 Rh6AG344500 Rh6BG351900 Rh6CG359000 Rh6CG359100 Rh6CG387600 Rh6CG480500 Rh6DG344600 Rh6DG467400
rosa_wichuraiana Rw0G010250 Rw0G014820 Rw0G021400 Rw0G023100 Rw1G009450 Rw1G012100 Rw1G012180 Rw1G028360 Rw2G054260 Rw3G024370 Rw3G024420 Rw3G024470 Rw5G010850 Rw5G011010 Rw5G011020 Rw5G011050 Rw5G011080 Rw5G011120 Rw5G012300 Rw5G018470 Rw5G033830 Rw6G008930 Rw6G030070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 222
AccB1I GGYRCC 3 cut(s) 222, 449, 959
AccI GTMKAC 2 cut(s) 354, 555
AclI AACGTT 1 cut(s) 1239
AclWI GGATC 3 cut(s) 140, 153, 633
AcoI YGGCCR 1 cut(s) 465
AcsI RAATTY 1 cut(s) 816
AcuI CTGAAG 1 cut(s) 891
AfaI GTAC 4 cut(s) 224, 347, 550, 763
AfiI CCNNNNNNNGG 2 cut(s) 736, 1189
AflII CTTAAG 2 cut(s) 491, 1250
AflIII ACRYGT 1 cut(s) 203
AgsI TTSAA 3 cut(s) 287, 1263, 1285
Alw26I GTCTC 1 cut(s) 713
AlwI GGATC 3 cut(s) 140, 153, 633
AlwNI CAGNNNCTG 1 cut(s) 842
Ama87I CYCGRG 1 cut(s) 65
AoxI GGCC 2 cut(s) 310, 465
ApeKI GCWGC 4 cut(s) 4, 75, 833, 1246
ApoI RAATTY 1 cut(s) 816
AseI ATTAAT 5 cut(s) 132, 326, 651, 678, 1227
Asp700I GAANNNNTTC 1 cut(s) 392
Asp718I GGTACC 1 cut(s) 222
AspLEI GCGC 1 cut(s) 1126
AspS9I GGNCC 2 cut(s) 1030, 1192
AsuHPI GGTGA 5 cut(s) 163, 469, 706, 745, 1219
AvaI CYCGRG 1 cut(s) 65
AvaII GGWCC 2 cut(s) 1030, 1192
BamHI GGATCC 1 cut(s) 145
BanI GGYRCC 3 cut(s) 222, 449, 959
BanII GRGCYC 2 cut(s) 66, 303
BarI GAAGNNNNNNTAC 2 cut(s) 717, 749
BbvI GCAGC 4 cut(s) 16, 62, 820, 1258
BccI CCATC 5 cut(s) 357, 893, 1208, 1345, 1505
BceAI ACGGC 1 cut(s) 480
BciVI GTATCC 1 cut(s) 1450
BclI TGATCA 1 cut(s) 1491
BcoDI GTCTC 1 cut(s) 713
BfaI CTAG 1 cut(s) 1160
BfmI CTRYAG 3 cut(s) 834, 999, 1078
BfoI RGCGCY 1 cut(s) 1127
BfrI CTTAAG 2 cut(s) 491, 1250
BfuI GTATCC 1 cut(s) 1450
BisI GCNGC 4 cut(s) 5, 76, 834, 1247
BlpI GCTNAGC 1 cut(s) 990
BlsI GCNGC 4 cut(s) 6, 77, 835, 1248
Bme18I GGWCC 2 cut(s) 1030, 1192
BmeT110I CYCGRG 1 cut(s) 65
BmgT120I GGNCC 2 cut(s) 1030, 1192
BmiI GGNNCC 7 cut(s) 147, 224, 451, 961, 975, 1032, 1487
BmsI GCATC 5 cut(s) 110, 167, 267, 342, 1369
BoxI GACNNNNGTC 1 cut(s) 725
BpmI CTGGAG 2 cut(s) 867, 949
Bpu10I CCTNAGC 1 cut(s) 585
Bpu1102I GCTNAGC 1 cut(s) 990
BpuEI CTTGAG 2 cut(s) 365, 1355
BsaAI YACGTR 1 cut(s) 204
BsaBI GATNNNNATC 1 cut(s) 930
BsaJI CCNNGG 1 cut(s) 1364
BsaWI WCCGGW 1 cut(s) 148
BsaXI ACNNNNNCTCC 2 cut(s) 1224, 1254
Bsc4I CCNNNNNNNGG 2 cut(s) 736, 1189
Bse1I ACTGG 4 cut(s) 741, 1054, 1431, 1502
Bse3DI GCAATG 1 cut(s) 1332
Bse8I GATNNNNATC 1 cut(s) 930
BseDI CCNNGG 1 cut(s) 1364
BseGI GGATG 7 cut(s) 55, 125, 396, 777, 931, 1356, 1360
BseJI GATNNNNATC 1 cut(s) 930
BseLI CCNNNNNNNGG 2 cut(s) 736, 1189
BseMI GCAATG 1 cut(s) 1332
BseMII CTCAG 2 cut(s) 1004, 1334
BseNI ACTGG 4 cut(s) 741, 1054, 1431, 1502
BseRI GAGGAG 1 cut(s) 1409
BseXI GCAGC 4 cut(s) 16, 62, 820, 1258
BshFI GGCC 2 cut(s) 312, 467
BshNI GGYRCC 3 cut(s) 222, 449, 959
BsiHKCI CYCGRG 1 cut(s) 65
BsiSI CCGG 2 cut(s) 149, 1142
BslFI GGGAC 2 cut(s) 713, 1353
BslI CCNNNNNNNGG 2 cut(s) 736, 1189
BsmAI GTCTC 1 cut(s) 713
BsmFI GGGAC 2 cut(s) 713, 1353
BsmI GAATGC 1 cut(s) 143
BsnI GGCC 2 cut(s) 312, 467
BsoBI CYCGRG 1 cut(s) 65
Bsp1286I GDGCHC 2 cut(s) 66, 303
Bsp143I GATC 5 cut(s) 10, 145, 625, 982, 1491
Bsp1720I GCTNAGC 1 cut(s) 990
Bsp19I CCATGG 1 cut(s) 1364
BspANI GGCC 2 cut(s) 312, 467
BspCNI CTCAG 2 cut(s) 1003, 1335
BspHI TCATGA 2 cut(s) 372, 1414
BspLI GGNNCC 7 cut(s) 147, 224, 451, 961, 975, 1032, 1487
BspMAI CTGCAG 1 cut(s) 838
BspPI GGATC 3 cut(s) 140, 153, 633
BspT107I GGYRCC 3 cut(s) 222, 449, 959
BspTI CTTAAG 2 cut(s) 491, 1250
BsrDI GCAATG 1 cut(s) 1332
BsrI ACTGG 4 cut(s) 741, 1054, 1431, 1502
BssECI CCNNGG 1 cut(s) 1364
BssMI GATC 5 cut(s) 10, 145, 625, 982, 1491
BssT1I CCWWGG 1 cut(s) 1364
Bst4CI ACNGT 3 cut(s) 553, 1003, 1018
BstAFI CTTAAG 2 cut(s) 491, 1250
BstAPI GCANNNNNTGC 1 cut(s) 842
BstBAI YACGTR 1 cut(s) 204
BstC8I GCNNGC 1 cut(s) 211
BstDEI CTNAG 4 cut(s) 585, 990, 1184, 1343
BstDSI CCRYGG 1 cut(s) 1364
BstENI CCTNNNNNAGG 1 cut(s) 1187
BstF5I GGATG 7 cut(s) 55, 125, 396, 777, 931, 1356, 1360
BstH2I RGCGCY 1 cut(s) 1127
BstHHI GCGC 1 cut(s) 1126
BstKTI GATC 5 cut(s) 13, 148, 628, 985, 1494
BstMAI GTCTC 1 cut(s) 713
BstMBI GATC 5 cut(s) 10, 145, 625, 982, 1491
BstMWI GCNNNNNNNGC 3 cut(s) 81, 376, 842
BstNSI RCATGY 1 cut(s) 28
BstPAI GACNNNNGTC 1 cut(s) 725
BstSFI CTRYAG 3 cut(s) 834, 999, 1078
BstV1I GCAGC 4 cut(s) 16, 62, 820, 1258
BstX2I RGATCY 1 cut(s) 145
BstXI CCANNNNNNTGG 2 cut(s) 233, 622
BstYI RGATCY 1 cut(s) 145
BsuI GTATCC 1 cut(s) 1450
BsuRI GGCC 2 cut(s) 312, 467
BtgI CCRYGG 1 cut(s) 1364
BtgZI GCGATG 1 cut(s) 244
BtsCI GGATG 7 cut(s) 55, 125, 396, 777, 931, 1356, 1360
BtsI GCAGTG 1 cut(s) 47
BtsIMutI CAGTG 5 cut(s) 47, 123, 520, 1008, 1424
Cac8I GCNNGC 1 cut(s) 211
CaiI CAGNNNCTG 1 cut(s) 842
CciI TCATGA 2 cut(s) 372, 1414
CfoI GCGC 1 cut(s) 1126
Cfr13I GGNCC 2 cut(s) 1030, 1192
Csp6I GTAC 4 cut(s) 223, 346, 549, 762
CviAII CATG 8 cut(s) 25, 373, 1165, 1312, 1365, 1415, 1442, 1480
CviQI GTAC 4 cut(s) 223, 346, 549, 762
DdeI CTNAG 4 cut(s) 585, 990, 1184, 1343
DpnI GATC 5 cut(s) 12, 147, 627, 984, 1493
DpnII GATC 5 cut(s) 10, 145, 625, 982, 1491
EaeI YGGCCR 1 cut(s) 465
Eco130I CCWWGG 1 cut(s) 1364
Eco24I GRGCYC 2 cut(s) 66, 303
Eco47I GGWCC 2 cut(s) 1030, 1192
Eco57I CTGAAG 1 cut(s) 891
Eco88I CYCGRG 1 cut(s) 65
EcoNI CCTNNNNNAGG 1 cut(s) 1187
EcoT14I CCWWGG 1 cut(s) 1364
EcoT22I ATGCAT 3 cut(s) 26, 182, 1362
EcoT38I GRGCYC 2 cut(s) 66, 303
ErhI CCWWGG 1 cut(s) 1364
FaeI CATG 8 cut(s) 28, 376, 1168, 1315, 1368, 1418, 1445, 1483
FaqI GGGAC 2 cut(s) 713, 1353
FatI CATG 8 cut(s) 24, 372, 1164, 1311, 1364, 1414, 1441, 1479
FbaI TGATCA 1 cut(s) 1491
FblI GTMKAC 2 cut(s) 354, 555
Fnu4HI GCNGC 4 cut(s) 5, 76, 834, 1247
FokI GGATG 7 cut(s) 62, 132, 383, 764, 938, 1347, 1363
FriOI GRGCYC 2 cut(s) 66, 303
Fsp4HI GCNGC 4 cut(s) 5, 76, 834, 1247
FspBI CTAG 1 cut(s) 1160
GlaI GCGC 1 cut(s) 1125
GluI GCNGC 4 cut(s) 5, 76, 834, 1247
GsuI CTGGAG 2 cut(s) 867, 949
HaeII RGCGCY 1 cut(s) 1127
HaeIII GGCC 2 cut(s) 312, 467
HapII CCGG 2 cut(s) 149, 1142
HhaI GCGC 1 cut(s) 1126
Hin1II CATG 8 cut(s) 28, 376, 1168, 1315, 1368, 1418, 1445, 1483
Hin6I GCGC 1 cut(s) 1124
HinP1I GCGC 1 cut(s) 1124
HincII GTYRAC 1 cut(s) 1059
HindII GTYRAC 1 cut(s) 1059
HindIII AAGCTT 2 cut(s) 698, 873
HinfI GANTC 5 cut(s) 103, 245, 525, 797, 1382
HpaII CCGG 2 cut(s) 149, 1142
HphI GGTGA 5 cut(s) 163, 469, 706, 745, 1219
Hpy166II GTNNAC 5 cut(s) 355, 556, 909, 1059, 1135
Hpy188I TCNGA 4 cut(s) 436, 871, 952, 982
Hpy188III TCNNGA 7 cut(s) 14, 55, 373, 607, 718, 966, 1415
Hpy8I GTNNAC 5 cut(s) 355, 556, 909, 1059, 1135
HpyAV CCTTC 7 cut(s) 83, 92, 490, 1114, 1286, 1309, 1341
HpyCH4III ACNGT 3 cut(s) 553, 1003, 1018
HpyCH4IV ACGT 2 cut(s) 203, 1239
HpyF10VI GCNNNNNNNGC 3 cut(s) 81, 376, 842
HpyF3I CTNAG 4 cut(s) 585, 990, 1184, 1343
HpySE526I ACGT 2 cut(s) 203, 1239
Hsp92II CATG 8 cut(s) 28, 376, 1168, 1315, 1368, 1418, 1445, 1483
HspAI GCGC 1 cut(s) 1124
KpnI GGTACC 1 cut(s) 226
Ksp22I TGATCA 1 cut(s) 1491
Kzo9I GATC 5 cut(s) 10, 145, 625, 982, 1491
LmnI GCTCC 2 cut(s) 1232, 1330
Lsp1109I GCAGC 4 cut(s) 16, 62, 820, 1258
LweI GCATC 5 cut(s) 110, 167, 267, 342, 1369
MaeI CTAG 1 cut(s) 1160
MaeII ACGT 2 cut(s) 203, 1239
MaeIII GTNAC 2 cut(s) 92, 751
MalI GATC 5 cut(s) 12, 147, 627, 984, 1493
MboI GATC 5 cut(s) 10, 145, 625, 982, 1491
MboII GAAGA 2 cut(s) 385, 466
MflI RGATCY 1 cut(s) 145
MhlI GDGCHC 2 cut(s) 66, 303
MluCI AATT 4 cut(s) 288, 644, 675, 816
MlyI GAGTC 1 cut(s) 239
MmeI TCCRAC 2 cut(s) 183, 825
Mph1103I ATGCAT 3 cut(s) 26, 182, 1362
MroXI GAANNNNTTC 1 cut(s) 392
MseI TTAA 9 cut(s) 132, 326, 419, 492, 651, 678, 824, 1227, 1251
MslI CAYNNNNRTG 5 cut(s) 231, 513, 687, 897, 1446
MspCI CTTAAG 2 cut(s) 491, 1250
MspI CCGG 2 cut(s) 149, 1142
Mva1269I GAATGC 1 cut(s) 143
MwoI GCNNNNNNNGC 3 cut(s) 81, 376, 842
NcoI CCATGG 1 cut(s) 1364
NdeII GATC 5 cut(s) 10, 145, 625, 982, 1491
NlaIII CATG 8 cut(s) 28, 376, 1168, 1315, 1368, 1418, 1445, 1483
NlaIV GGNNCC 7 cut(s) 147, 224, 451, 961, 975, 1032, 1487
NmuCI GTSAC 1 cut(s) 751
NsiI ATGCAT 3 cut(s) 26, 182, 1362
NspI RCATGY 1 cut(s) 28
PagI TCATGA 2 cut(s) 372, 1414
PcsI WCGNNNNNNNCGW 1 cut(s) 93
PctI GAATGC 1 cut(s) 143
PdmI GAANNNNTTC 1 cut(s) 392
PfeI GAWTC 4 cut(s) 103, 525, 797, 1382
PkrI GCNGC 4 cut(s) 6, 77, 835, 1248
PleI GAGTC 1 cut(s) 239
PpsI GAGTC 1 cut(s) 239
Ppu21I YACGTR 1 cut(s) 204
PshAI GACNNNNGTC 1 cut(s) 725
PshBI ATTAAT 5 cut(s) 132, 326, 651, 678, 1227
Psp1406I AACGTT 1 cut(s) 1239
PspN4I GGNNCC 7 cut(s) 147, 224, 451, 961, 975, 1032, 1487
PspPI GGNCC 2 cut(s) 1030, 1192
PstI CTGCAG 1 cut(s) 838
PstNI CAGNNNCTG 1 cut(s) 842
PsuI RGATCY 1 cut(s) 145
RsaI GTAC 4 cut(s) 224, 347, 550, 763
RsaNI GTAC 4 cut(s) 223, 346, 549, 762
RseI CAYNNNNRTG 5 cut(s) 231, 513, 687, 897, 1446
SaqAI TTAA 9 cut(s) 132, 326, 419, 492, 651, 678, 824, 1227, 1251
SatI GCNGC 4 cut(s) 5, 76, 834, 1247
Sau3AI GATC 5 cut(s) 10, 145, 625, 982, 1491
Sau96I GGNCC 2 cut(s) 1030, 1192
SchI GAGTC 1 cut(s) 239
SduI GDGCHC 2 cut(s) 66, 303
SfaNI GCATC 5 cut(s) 110, 167, 267, 342, 1369
SfcI CTRYAG 3 cut(s) 834, 999, 1078
SinI GGWCC 2 cut(s) 1030, 1192
SmiMI CAYNNNNRTG 5 cut(s) 231, 513, 687, 897, 1446
SmlI CTYRAG 4 cut(s) 380, 491, 1250, 1334
SmoI CTYRAG 4 cut(s) 380, 491, 1250, 1334
Sse9I AATT 4 cut(s) 288, 644, 675, 816
SspI AATATT 1 cut(s) 279
SspMI CTAG 1 cut(s) 1160
StyI CCWWGG 1 cut(s) 1364
TaaI ACNGT 3 cut(s) 553, 1003, 1018
TaiI ACGT 2 cut(s) 206, 1242
TaqI TCGA 3 cut(s) 628, 717, 771
TaqII GACCGA 1 cut(s) 1209
TasI AATT 4 cut(s) 288, 644, 675, 816
TatI WGTACW 1 cut(s) 345
TfiI GAWTC 4 cut(s) 103, 525, 797, 1382
Tru1I TTAA 9 cut(s) 132, 326, 419, 492, 651, 678, 824, 1227, 1251
Tru9I TTAA 9 cut(s) 132, 326, 419, 492, 651, 678, 824, 1227, 1251
TscAI CASTG 5 cut(s) 47, 130, 520, 1008, 1431
TseFI GTSAC 1 cut(s) 751
TseI GCWGC 4 cut(s) 4, 75, 833, 1246
Tsp45I GTSAC 1 cut(s) 751
TspGWI ACGGA 1 cut(s) 966
TspRI CASTG 5 cut(s) 47, 130, 520, 1008, 1431
Vha464I CTTAAG 2 cut(s) 491, 1250
VpaK11BI GGWCC 2 cut(s) 1030, 1192
VspI ATTAAT 5 cut(s) 132, 326, 651, 678, 1227
XagI CCTNNNNNAGG 1 cut(s) 1187
XapI RAATTY 1 cut(s) 816
XceI RCATGY 1 cut(s) 28
XcmI CCANNNNNNNNNTGG 1 cut(s) 1318
XmiI GTMKAC 2 cut(s) 354, 555
XmnI GAANNNNTTC 1 cut(s) 392
XspI CTAG 1 cut(s) 1160
Zsp2I ATGCAT 3 cut(s) 26, 182, 1362
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.