MD10G1249500.v1.1

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000066
Physical Location & Seq
Forward (+)
34212764 .. 34214829
2066 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1249500.v1.1.491

Sequence Viewer

Length: 1281 bp
ATGGAAGTGTATGGGAAGTGGAAGAGTAGAAAATCCATCTCCTTTTTGCCACTCCACTGTGCCAGTGGAGGCTTCTTGCTTTTACTAAGTGGATCTTGGATATATTGGGGAATGCAGAGAAGAAAGTTCCTCAAACTCAAAGAAAAATACTTTAAAGAAAATGGTGGCTTATTGTTACAACAAAAAATCGCTAGTCAAGGAGGCTCTATGGAGACAACAAAAATTTTCACGGCAGAAGAACTTGAGAAGGCAACAAACAATTACCATGAAAGTGGAATCCTTGGTGAAGGAGGCTATGGAATAGTTTACAAAGGAATATTAGCAGCAAATAACAATAAAGTGGTTGCCATAAAAAAGTCTAAAATTTGTGTCCTAACGCAGAAGGAGCAGTTTGTCAACGAGTTGCTTGTTCTTTCTCAAATCAACCATAGAAATGTGGTGAGGTTATTGGGTTGTTGTTTGGAGTTAGAAGTTCCTCTACTAGTTTACGAGTTTGTCGCTCACGGCACTCTCTTTGAGCACATACATGGCAAAAAGAGGAAAGGATCATCATTTTCATTGGAATTACGACTCAAGATAGCAGCTGAAATTGCTGGAGCACTAGCATACTTACACTCCTCAGCTATGATGCAAATCATACATCGAGATGTGAAACTGACAAATATACTGTTAGATGAAAATTACACGGCAAAAGTGTCAGACTTTGGAGCTTCACGATTGATTCCTCTTGATCAAGCCCAACTTGCAACTTTAGTGCAAGGAATAGTCGGATACTTAGACCCTGAATACTTCCTCACGAGTCAACTAACAGATAAGAGTGACGTCTATAGCTTTGGAGTTGTCCTTATGGAGCTACTAACAAGTAAACTGGCACTTGATTCTGACAGGCCTGGGGCAGATAGAAGCCTAGCAAGGTTCTTTGTTTGTTTAATGGAGGAGGATCGCCTGAATGAAATTCTCGATGATGACATGCTCAACGAGAGAAACATCGAGACACTAAAAACAGTGGCCAATCTAGCAAAAAGATGTGTAAGGGTAAAAGGGAAGGACAGGCCAACAATGAAAGAAGTTGCCATGGAGCTGGAAGGGATGAGAATTACAGAAAAGCATCCATGGGGAAAAGCTGAAACTTGTTCAAAAGAGATTGAGTGCTTACTTGGGCCAGGTAACTCAGATGCTTATCATATGGATGTTAGAGCTGATTGTGGTCCTAGTACTGGTACAACTATTGGGTATGACAGCATGCATATCCAGTTTACTCCATATGATGATGGACGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK

Protein Analysis

427

Amino Acids

47.76

Weight (kDa)

7.15

Isoelectric Point (pI)

27.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 88 - 357 8.7e-45 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 93 - 361 4.4e-47 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21210 AT1G21230 AT1G21240 AT1G21240 AT1G21245 AT1G21250 AT1G21270 AT1G22720 AT3G25490
fragaria_vesca FvH4_2g35051 FvH4_2g35051 FvH4_2g35060 FvH4_3g10281 FvH4_3g10285 FvH4_3g10286 FvH4_3g10311 FvH4_3g10311 FvH4_3g10320 FvH4_3g10332 FvH4_3g10340 FvH4_3g10350 FvH4_3g10360 FvH4_3g10390 FvH4_3g10412 FvH4_3g10413 FvH4_3g10461 FvH4_3g10462 FvH4_3g10470 FvH4_3g10510 FvH4_3g10520 FvH4_3g10530 FvH4_3g10550 FvH4_3g10560 FvH4_3g10611 FvH4_3g11730 FvH4_6g53140 FvH4_6g53142 FvH4_7g31880 FvH4_7g31910 FvH4_7g31920
malus_domestica MD05G1269900.v1.1 MD05G1270000.v1.1 MD05G1270200.v1.1 MD05G1270400.v1.1 MD05G1270500.v1.1 MD05G1270600.v1.1 MD05G1270800.v1.1 MD05G1270900.v1.1 MD07G1183800.v1.1 MD08G1107600.v1.1 MD10G1248500.v1.1 MD10G1249200.v1.1 MD10G1249500.v1.1 MD10G1250000.v1.1 MD10G1250500.v1.1 MD10G1250900.v1.1 MD10G1251200.v1.1 MD10G1251400.v1.1 MD13G1004600.v1.1 MD15G1088400.v1.1
prunus_persica Prupe.1G442400_v2.0.a1 Prupe.1G442500_v2.0.a1 Prupe.2G223400_v2.0.a1 Prupe.4G091900_v2.0.a1 Prupe.4G093200_v2.0.a1 Prupe.4G093300_v2.0.a1 Prupe.4G093400_v2.0.a1 Prupe.4G093500_v2.0.a1 Prupe.4G094100_v2.0.a1 Prupe.4G094200_v2.0.a1 Prupe.4G094300_v2.0.a1 Prupe.6G358300_v2.0.a1
pyrus_communis pycom08g08990 pycom10g20660 pycom10g20670 pycom10g20680 pycom10g20710 pycom10g20740 pycom13g00400 pycom15g08290
rosa_chinensis RchiOBHm_Chr1g0326861 RchiOBHm_Chr1g0326871 RchiOBHm_Chr1g0331391 RchiOBHm_Chr1g0336911 RchiOBHm_Chr1g0364001 RchiOBHm_Chr2g0174541 RchiOBHm_Chr2g0175041 RchiOBHm_Chr2g0175101 RchiOBHm_Chr2g0175121 RchiOBHm_Chr3g0486331 RchiOBHm_Chr5g0015581 RchiOBHm_Chr5g0016551 RchiOBHm_Chr5g0016561 RchiOBHm_Chr5g0016581 RchiOBHm_Chr5g0016601 RchiOBHm_Chr5g0016611 RchiOBHm_Chr5g0016641 RchiOBHm_Chr5g0016661 RchiOBHm_Chr5g0016691 RchiOBHm_Chr5g0016701 RchiOBHm_Chr5g0016731 RchiOBHm_Chr5g0016741 RchiOBHm_Chr5g0016811 RchiOBHm_Chr5g0016841 RchiOBHm_Chr5g0016871 RchiOBHm_Chr5g0016881 RchiOBHm_Chr5g0016931 RchiOBHm_Chr5g0017021 RchiOBHm_Chr5g0017041 RchiOBHm_Chr5g0017071 RchiOBHm_Chr5g0017091 RchiOBHm_Chr5g0017121 RchiOBHm_Chr5g0017141 RchiOBHm_Chr5g0017291 RchiOBHm_Chr5g0017321 RchiOBHm_Chr5g0017401 RchiOBHm_Chr5g0019001 RchiOBHm_Chr5g0019071 RchiOBHm_Chr5g0028941 RchiOBHm_Chr5g0052141 RchiOBHm_Chr5g0054721 RchiOBHm_Chr5g0054801 RchiOBHm_Chr5g0054931 RchiOBHm_Chr5g0054941 RchiOBHm_Chr6g0259291 RchiOBHm_Chr6g0292991 RchiOBHm_Chr6g0306911
rosa_laevigata RLG00000009465 RLG00000009468 RLG00000010755 RLG00000011976 RLG00000014541 RLG00000014542 RLG00000015169 RLG00000022308 RLG00000027529 RLG00000029324 RLG00000030244 RLG00000030245 RLG00000032210 RLG00000032211 RLG00000032257 RLG00000032259 RLG00000032260 RLG00000032261 RLG00000032262 RLG00000032268 RLG00000032269 RLG00000032272 RLG00000032282 RLG00000032283 RLG00000032308 RLG00000032413 RLG00000033129 RLG00000034945
rosa_multiflora Rmu_co8241929.1_g000001 Rmu_co8269227.1_g000001 Rmu_co8461259.1_g000001 Rmu_co8495385.1_g000001 Rmu_sc0000028.1_g000003 Rmu_sc0000083.1_g000009 Rmu_sc0000083.1_g000010 Rmu_sc0000083.1_g000031 Rmu_sc0000083.1_g000043 Rmu_sc0000083.1_g000046 Rmu_sc0000083.1_g000047 Rmu_sc0000563.1_g000024 Rmu_sc0000842.1_g000012 Rmu_sc0001235.1_g000001 Rmu_sc0001235.1_g000005 Rmu_sc0001235.1_g000009 Rmu_sc0001235.1_g000015 Rmu_sc0001235.1_g000016 Rmu_sc0001235.1_g000017 Rmu_sc0001473.1_g000036 Rmu_sc0001607.1_g000016 Rmu_sc0001788.1_g000006 Rmu_sc0002018.1_g000008 Rmu_sc0002028.1_g000001 Rmu_sc0002680.1_g000011 Rmu_sc0002798.1_g000023 Rmu_sc0002886.1_g000023 Rmu_sc0002989.1_g000012 Rmu_sc0003064.1_g000009 Rmu_sc0003382.1_g000006 Rmu_sc0003630.1_g000002 Rmu_sc0003630.1_g000047 Rmu_sc0003630.1_g000066 Rmu_sc0003630.1_g000067 Rmu_sc0003649.1_g000010 Rmu_sc0003688.1_g000005 Rmu_sc0003920.1_g000007 Rmu_sc0004765.1_g000007 Rmu_sc0004765.1_g000008 Rmu_sc0004920.1_g000011 Rmu_sc0005223.1_g000008 Rmu_sc0005223.1_g000010 Rmu_sc0005223.1_g000015 Rmu_sc0005223.1_g000016 Rmu_sc0005319.1_g000014 Rmu_sc0005319.1_g000015 Rmu_sc0005319.1_g000018 Rmu_sc0005319.1_g000019 Rmu_sc0005319.1_g000025 Rmu_sc0005418.1_g000007 Rmu_sc0005418.1_g000012 Rmu_sc0006106.1_g000011 Rmu_sc0006914.1_g000012 Rmu_sc0007023.1_g000001 Rmu_sc0007662.1_g000001 Rmu_sc0007920.1_g000002 Rmu_sc0007920.1_g000013 Rmu_sc0008053.1_g000016 Rmu_sc0008053.1_g000017 Rmu_sc0008053.1_g000018 Rmu_sc0008053.1_g000023 Rmu_sc0008053.1_g000050 Rmu_sc0009042.1_g000007 Rmu_sc0009042.1_g000008 Rmu_sc0010642.1_g000008 Rmu_sc0011109.1_g000002 Rmu_sc0011314.1_g000006 Rmu_sc0011974.1_g000006 Rmu_sc0012179.1_g000009 Rmu_sc0015169.1_g000001 Rmu_sc0016368.1_g000002 Rmu_sc0022659.1_g000001 Rmu_sc0027371.1_g000001 Rmu_sc0028213.1_g000001 Rmu_sc0034526.1_g000001 Rmu_sc0039366.1_g000002 Rmu_ssc0000050.1_g000084 Rmu_ssc0000050.1_g000088 Rmu_ssc0000167.1_g000017
rosa_roxburghii Rroxscaffold_1G00011340 Rroxscaffold_1G00025710 Rroxscaffold_1G00025870 Rroxscaffold_1G00026070 Rroxscaffold_1G00026170 Rroxscaffold_1G00026200 Rroxscaffold_1G00026270 Rroxscaffold_1G00050950 Rroxscaffold_1G00058900 Rroxscaffold_1G00060040 Rroxscaffold_1G00060060 Rroxscaffold_1G00060070 Rroxscaffold_1G00060080 Rroxscaffold_1G00060110 Rroxscaffold_1G00060120 Rroxscaffold_1G00060140 Rroxscaffold_1G00060170 Rroxscaffold_1G00060210 Rroxscaffold_1G00060220 Rroxscaffold_1G00060240 Rroxscaffold_1G00060250 Rroxscaffold_1G00060260 Rroxscaffold_1G00060300 Rroxscaffold_1G00060320 Rroxscaffold_1G00060330 Rroxscaffold_1G00060900 Rroxscaffold_1G00060920 Rroxscaffold_1G00073980 Rroxscaffold_2G00077560 Rroxscaffold_2G00077580 Rroxscaffold_2G00146990 Rroxscaffold_3G00236200 Rroxscaffold_3G00236210 Rroxscaffold_3G00236220 Rroxscaffold_4G00292760 Rroxscaffold_4G00315200 Rroxscaffold_4G00315240 Rroxscaffold_4G00326240 Rroxscaffold_4G00328980 Rroxscaffold_5G00341890 Rroxscaffold_7G00161350 Rroxscaffold_7G00174490 Rroxscaffold_7G00206550
rosa_rugosa Rorug01G0093000 Rorug01G0093100 Rorug01G0128800 Rorug01G0312000 Rorug01G0312000 Rorug02G0581100 Rorug03G0224600 Rorug05G0028500 Rorug05G0032700 Rorug05G0032800 Rorug05G0032900 Rorug05G0032900 Rorug05G0033000 Rorug05G0033100 Rorug05G0033300 Rorug05G0033400 Rorug05G0033900 Rorug05G0034900 Rorug05G0035000 Rorug05G0046900 Rorug05G0047500 Rorug05G0047500 Rorug05G0111600 Rorug05G0287900 Rorug05G0288700 Rorug05G0586900 Rorug06G0231700 Rorug06G0354300 Rorug06G0354400
rosa_samantha Rh1DG122200 Rh2DG686800 Rh5BG136600 Rh6AG344500 Rh6BG351900 Rh6CG359000 Rh6CG359100 Rh6CG387600 Rh6CG480500 Rh6DG344600 Rh6DG467400
rosa_wichuraiana Rw0G010250 Rw0G014820 Rw0G021400 Rw0G023100 Rw1G009450 Rw1G012100 Rw1G012180 Rw1G028360 Rw2G054260 Rw3G024370 Rw3G024420 Rw3G024470 Rw5G010850 Rw5G011010 Rw5G011020 Rw5G011050 Rw5G011080 Rw5G011120 Rw5G012300 Rw5G018470 Rw5G033830 Rw6G008930 Rw6G030070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 825
AclWI GGATC 3 cut(s) 100, 553, 948
AcoI YGGCCR 1 cut(s) 1008
AcsI RAATTY 3 cut(s) 222, 363, 954
AcyI GRCGYC 1 cut(s) 822
AfaI GTAC 2 cut(s) 1216, 1222
AfiI CCNNNNNNNGG 1 cut(s) 1217
AgsI TTSAA 1 cut(s) 1137
AhlI ACTAGT 1 cut(s) 481
AjnI CCWGG 2 cut(s) 889, 1162
AluBI AGCT 8 cut(s) 584, 623, 710, 831, 853, 1081, 1124, 1199
AluI AGCT 8 cut(s) 584, 623, 710, 831, 853, 1081, 1124, 1199
Alw21I GWGCWC 2 cut(s) 522, 601
Alw26I GTCTC 2 cut(s) 206, 986
AlwI GGATC 3 cut(s) 100, 553, 948
AoxI GGCC 4 cut(s) 887, 1008, 1052, 1160
ApeKI GCWGC 2 cut(s) 323, 581
ApoI RAATTY 3 cut(s) 222, 363, 954
AspS9I GGNCC 2 cut(s) 1160, 1208
AsuHPI GGTGA 2 cut(s) 296, 451
AvaII GGWCC 1 cut(s) 1208
BalI TGGCCA 1 cut(s) 1010
BarI GAAGNNNNNNTAC 2 cut(s) 462, 494
BauI CACGAG 1 cut(s) 796
Bbv12I GWGCWC 2 cut(s) 522, 601
BbvCI CCTCAGC 1 cut(s) 619
BbvI GCAGC 2 cut(s) 335, 593
BccI CCATC 2 cut(s) 44, 1265
BceAI ACGGC 3 cut(s) 246, 520, 702
BciT130I CCWGG 2 cut(s) 891, 1164
BciVI GTATCC 1 cut(s) 764
BclI TGATCA 1 cut(s) 730
BcoDI GTCTC 2 cut(s) 206, 986
BcuI ACTAGT 1 cut(s) 481
BfaI CTAG 6 cut(s) 192, 482, 602, 908, 1016, 1212
BfmI CTRYAG 1 cut(s) 826
BfuI GTATCC 1 cut(s) 764
BisI GCNGC 2 cut(s) 324, 582
BlsI GCNGC 2 cut(s) 325, 583
BmcAI AGTACT 1 cut(s) 1216
Bme1390I CCNGG 2 cut(s) 891, 1164
Bme18I GGWCC 1 cut(s) 1208
BmgT120I GGNCC 2 cut(s) 1160, 1208
BmrFI CCNGG 2 cut(s) 891, 1164
BmsI GCATC 3 cut(s) 618, 1117, 1165
BpmI CTGGAG 1 cut(s) 615
Bpu10I CCTNAGC 1 cut(s) 619
BpuEI CTTGAG 2 cut(s) 263, 557
BsaBI GATNNNNATC 2 cut(s) 632, 1179
BsaHI GRCGYC 1 cut(s) 822
BsaJI CCNNGG 4 cut(s) 280, 890, 1074, 1112
BsaXI ACNNNNNCTCC 2 cut(s) 599, 629
Bsc4I CCNNNNNNNGG 1 cut(s) 1217
Bse1I ACTGG 4 cut(s) 63, 873, 1222, 1252
Bse8I GATNNNNATC 2 cut(s) 632, 1179
BseBI CCWGG 2 cut(s) 891, 1164
BseDI CCNNGG 4 cut(s) 280, 890, 1074, 1112
BseGI GGATG 3 cut(s) 1095, 1108, 1195
BseJI GATNNNNATC 2 cut(s) 632, 1179
BseLI CCNNNNNNNGG 1 cut(s) 1217
BseMII CTCAG 2 cut(s) 633, 1185
BseNI ACTGG 4 cut(s) 63, 873, 1222, 1252
BseRI GAGGAG 2 cut(s) 607, 950
BseXI GCAGC 2 cut(s) 335, 593
BshFI GGCC 4 cut(s) 889, 1010, 1054, 1162
BsiHKAI GWGCWC 2 cut(s) 522, 601
BslI CCNNNNNNNGG 1 cut(s) 1217
BsmAI GTCTC 2 cut(s) 206, 986
BsmI GAATGC 1 cut(s) 117
BsnI GGCC 4 cut(s) 889, 1010, 1054, 1162
Bsp1286I GDGCHC 2 cut(s) 522, 601
Bsp143I GATC 4 cut(s) 92, 545, 730, 940
Bsp19I CCATGG 2 cut(s) 1074, 1112
BspANI GGCC 4 cut(s) 889, 1010, 1054, 1162
BspCNI CTCAG 2 cut(s) 632, 1184
BspPI GGATC 3 cut(s) 100, 553, 948
BsrI ACTGG 4 cut(s) 63, 873, 1222, 1252
BssECI CCNNGG 4 cut(s) 280, 890, 1074, 1112
BssMI GATC 4 cut(s) 92, 545, 730, 940
BssNI GRCGYC 1 cut(s) 822
BssSI CACGAG 1 cut(s) 796
BssT1I CCWWGG 3 cut(s) 280, 1074, 1112
Bst2BI CACGAG 1 cut(s) 796
Bst2UI CCWGG 2 cut(s) 891, 1164
Bst4CI ACNGT 3 cut(s) 59, 669, 1006
Bst6I CTCTTC 1 cut(s) 17
BstACI GRCGYC 1 cut(s) 822
BstC8I GCNNGC 1 cut(s) 1244
BstDEI CTNAG 4 cut(s) 86, 619, 775, 1171
BstDSI CCRYGG 2 cut(s) 1074, 1112
BstF5I GGATG 3 cut(s) 1095, 1108, 1195
BstKTI GATC 4 cut(s) 95, 548, 733, 943
BstMAI GTCTC 2 cut(s) 206, 986
BstMBI GATC 4 cut(s) 92, 545, 730, 940
BstMWI GCNNNNNNNGC 4 cut(s) 385, 590, 743, 1016
BstNI CCWGG 2 cut(s) 891, 1164
BstNSI RCATGY 2 cut(s) 973, 1246
BstSCI CCNGG 2 cut(s) 889, 1162
BstSFI CTRYAG 1 cut(s) 826
BstV1I GCAGC 2 cut(s) 335, 593
BstX2I RGATCY 1 cut(s) 92
BstXI CCANNNNNNTGG 2 cut(s) 272, 1081
BstYI RGATCY 1 cut(s) 92
BsuI GTATCC 1 cut(s) 764
BsuRI GGCC 4 cut(s) 889, 1010, 1054, 1162
BtgI CCRYGG 2 cut(s) 1074, 1112
BtsCI GGATG 3 cut(s) 1095, 1108, 1195
BtsIMutI CAGTG 3 cut(s) 55, 70, 1011
Cac8I GCNNGC 1 cut(s) 1244
Cfr13I GGNCC 2 cut(s) 1160, 1208
Csp6I GTAC 2 cut(s) 1215, 1221
CviAII CATG 6 cut(s) 266, 527, 970, 1075, 1113, 1243
CviQI GTAC 2 cut(s) 1215, 1221
DdeI CTNAG 4 cut(s) 86, 619, 775, 1171
DpnI GATC 4 cut(s) 94, 547, 732, 942
DpnII GATC 4 cut(s) 92, 545, 730, 940
DraI TTTAAA 1 cut(s) 154
EaeI YGGCCR 1 cut(s) 1008
Eam1104I CTCTTC 1 cut(s) 17
EarI CTCTTC 1 cut(s) 17
Eco130I CCWWGG 3 cut(s) 280, 1074, 1112
Eco147I AGGCCT 1 cut(s) 889
Eco47I GGWCC 1 cut(s) 1208
EcoRII CCWGG 2 cut(s) 889, 1162
EcoT14I CCWWGG 3 cut(s) 280, 1074, 1112
EcoT22I ATGCAT 1 cut(s) 1248
ErhI CCWWGG 3 cut(s) 280, 1074, 1112
FaeI CATG 6 cut(s) 269, 530, 973, 1078, 1116, 1246
FalI AAGNNNNNCTT 4 cut(s) 79, 111, 726, 758
FatI CATG 6 cut(s) 265, 526, 969, 1074, 1112, 1242
FauNDI CATATG 2 cut(s) 1185, 1264
FbaI TGATCA 1 cut(s) 730
Fnu4HI GCNGC 2 cut(s) 324, 582
FokI GGATG 3 cut(s) 1095, 1102, 1202
Fsp4HI GCNGC 2 cut(s) 324, 582
FspBI CTAG 6 cut(s) 192, 482, 602, 908, 1016, 1212
GluI GCNGC 2 cut(s) 324, 582
GsuI CTGGAG 1 cut(s) 615
HaeIII GGCC 4 cut(s) 889, 1010, 1054, 1162
Hin1I GRCGYC 1 cut(s) 822
Hin1II CATG 6 cut(s) 269, 530, 973, 1078, 1116, 1246
HincII GTYRAC 2 cut(s) 397, 803
HindII GTYRAC 2 cut(s) 397, 803
HinfI GANTC 5 cut(s) 276, 570, 721, 799, 878
HphI GGTGA 2 cut(s) 296, 451
Hpy166II GTNNAC 6 cut(s) 307, 397, 487, 803, 866, 1257
Hpy188I TCNGA 4 cut(s) 700, 770, 883, 1174
Hpy188III TCNNGA 7 cut(s) 574, 644, 714, 728, 796, 959, 991
Hpy8I GTNNAC 6 cut(s) 307, 397, 487, 803, 866, 1257
HpyAV CCTTC 5 cut(s) 241, 281, 376, 1039, 1079
HpyCH4III ACNGT 3 cut(s) 59, 669, 1006
HpyCH4IV ACGT 1 cut(s) 822
HpyCH4V TGCA 5 cut(s) 115, 631, 746, 757, 1246
HpyF10VI GCNNNNNNNGC 4 cut(s) 385, 590, 743, 1016
HpyF3I CTNAG 4 cut(s) 86, 619, 775, 1171
HpySE526I ACGT 1 cut(s) 822
Hsp92I GRCGYC 1 cut(s) 822
Hsp92II CATG 6 cut(s) 269, 530, 973, 1078, 1116, 1246
Ksp22I TGATCA 1 cut(s) 730
Kzo9I GATC 4 cut(s) 92, 545, 730, 940
LmnI GCTCC 5 cut(s) 385, 596, 707, 850, 1078
Lsp1109I GCAGC 2 cut(s) 335, 593
LweI GCATC 3 cut(s) 618, 1117, 1165
MaeI CTAG 6 cut(s) 192, 482, 602, 908, 1016, 1212
MaeII ACGT 1 cut(s) 822
MaeIII GTNAC 3 cut(s) 174, 818, 1166
MalI GATC 4 cut(s) 94, 547, 732, 942
MboI GATC 4 cut(s) 92, 545, 730, 940
MboII GAAGA 3 cut(s) 34, 132, 248
MflI RGATCY 1 cut(s) 92
MhlI GDGCHC 2 cut(s) 522, 601
MlsI TGGCCA 1 cut(s) 1010
MluCI AATT 8 cut(s) 222, 259, 363, 563, 588, 679, 954, 1095
MluNI TGGCCA 1 cut(s) 1010
MlyI GAGTC 2 cut(s) 564, 808
MmeI TCCRAC 1 cut(s) 748
Mox20I TGGCCA 1 cut(s) 1010
Mph1103I ATGCAT 1 cut(s) 1248
MscI TGGCCA 1 cut(s) 1010
MseI TTAA 2 cut(s) 153, 929
MslI CAYNNNNRTG 5 cut(s) 270, 432, 525, 645, 1188
Msp20I TGGCCA 1 cut(s) 1010
MspA1I CMGCKG 1 cut(s) 584
MspR9I CCNGG 2 cut(s) 891, 1164
Mva1269I GAATGC 1 cut(s) 117
MvaI CCWGG 2 cut(s) 891, 1164
MwoI GCNNNNNNNGC 4 cut(s) 385, 590, 743, 1016
NcoI CCATGG 2 cut(s) 1074, 1112
NdeI CATATG 2 cut(s) 1185, 1264
NdeII GATC 4 cut(s) 92, 545, 730, 940
NlaIII CATG 6 cut(s) 269, 530, 973, 1078, 1116, 1246
NmuCI GTSAC 1 cut(s) 818
NsiI ATGCAT 1 cut(s) 1248
NspI RCATGY 2 cut(s) 973, 1246
PaeI GCATGC 1 cut(s) 1246
PceI AGGCCT 1 cut(s) 889
PctI GAATGC 1 cut(s) 117
PfeI GAWTC 3 cut(s) 276, 721, 878
PkrI GCNGC 2 cut(s) 325, 583
PleI GAGTC 2 cut(s) 564, 807
PpsI GAGTC 2 cut(s) 564, 807
Psp6I CCWGG 2 cut(s) 889, 1162
PspGI CCWGG 2 cut(s) 889, 1162
PspPI GGNCC 2 cut(s) 1160, 1208
PsuI RGATCY 1 cut(s) 92
PvuII CAGCTG 1 cut(s) 584
RsaI GTAC 2 cut(s) 1216, 1222
RsaNI GTAC 2 cut(s) 1215, 1221
RseI CAYNNNNRTG 5 cut(s) 270, 432, 525, 645, 1188
SaqAI TTAA 2 cut(s) 153, 929
SatI GCNGC 2 cut(s) 324, 582
Sau3AI GATC 4 cut(s) 92, 545, 730, 940
Sau96I GGNCC 2 cut(s) 1160, 1208
ScaI AGTACT 1 cut(s) 1216
SchI GAGTC 2 cut(s) 564, 808
ScrFI CCNGG 2 cut(s) 891, 1164
SduI GDGCHC 2 cut(s) 522, 601
SfaNI GCATC 3 cut(s) 618, 1117, 1165
SfcI CTRYAG 1 cut(s) 826
SinI GGWCC 1 cut(s) 1208
SmiMI CAYNNNNRTG 5 cut(s) 270, 432, 525, 645, 1188
SmlI CTYRAG 2 cut(s) 242, 572
SmoI CTYRAG 2 cut(s) 242, 572
SpeI ACTAGT 1 cut(s) 481
SphI GCATGC 1 cut(s) 1246
Sse9I AATT 8 cut(s) 222, 259, 363, 563, 588, 679, 954, 1095
SseBI AGGCCT 1 cut(s) 889
SspI AATATT 1 cut(s) 318
SspMI CTAG 6 cut(s) 192, 482, 602, 908, 1016, 1212
StuI AGGCCT 1 cut(s) 889
StyD4I CCNGG 2 cut(s) 889, 1162
StyI CCWWGG 3 cut(s) 280, 1074, 1112
TaaI ACNGT 3 cut(s) 59, 669, 1006
TaiI ACGT 1 cut(s) 825
TaqI TCGA 3 cut(s) 643, 960, 990
TasI AATT 8 cut(s) 222, 259, 363, 563, 588, 679, 954, 1095
TatI WGTACW 1 cut(s) 1214
TfiI GAWTC 3 cut(s) 276, 721, 878
Tru1I TTAA 2 cut(s) 153, 929
Tru9I TTAA 2 cut(s) 153, 929
TscAI CASTG 3 cut(s) 62, 70, 1011
TseFI GTSAC 1 cut(s) 818
TseI GCWGC 2 cut(s) 323, 581
Tsp45I GTSAC 1 cut(s) 818
TspDTI ATGAA 5 cut(s) 282, 546, 690, 966, 1076
TspRI CASTG 3 cut(s) 62, 70, 1011
VpaK11BI GGWCC 1 cut(s) 1208
XapI RAATTY 3 cut(s) 222, 363, 954
XceI RCATGY 2 cut(s) 973, 1246
XcmI CCANNNNNNNNNTGG 1 cut(s) 62
XspI CTAG 6 cut(s) 192, 482, 602, 908, 1016, 1212
ZraI GACGTC 1 cut(s) 823
ZrmI AGTACT 1 cut(s) 1216
Zsp2I ATGCAT 1 cut(s) 1248
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.