Rorug06G0354300

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0000066
Physical Location & Seq
Reverse (-)
50233978 .. 50238668
4691 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0354300.1

Sequence Viewer

Length: 2703 bp
ATGGCAGAGACCAAGCGAAAAACCAGAGACGACGGAAGTAGACAGCGGAGCAGCAGCAAGAAGCCGAAAACCAGCAAGAAAGGCAAGAGCGCCGATAAGGCAGAAAGAAAAGGCCCTCGCTTGCCCAACGCTCTGCGGAAGGAGCTCGACCGCTTAAACCCTAGAACCGCCGACTCCGACGAAGAGATTGGCTCCGATGAAGAGGCTTTGGGCCAGGACCTGTATGAGTATGAAGAGGGACTGCCGGAGGAGGAGTCCAAGAAGAACCGCCGGTTCGATCCCGTCGATAACCTCGATTATGAGCTACCTGAGGACTTCGAGGATGAAAATGTGTCATCTGATGATGACAATGAGGCCAGGAACGGTGGAGAGAGTGAGGCGGAGGACGAGGTTAAAGAGGAAAATGATGGAAGCAGAATCAGAGAAATGCCGGCCGAGACTTTTGAAGGGAGGAGGAAGAAGATAAACAATGTTGTGGTGTCGGAGGCATATCCCGAGTCCGAGTATAACCCTACTCGTGATATGTTGGAGGGTGAAGGAGCACTTAGCATTGGAGACCTTCTGGACCCTCTCCAAGGGAAACATGGTTATAGCAAACTTAAGAAGAGAGTACGTATTCTGGATAAGAAATCTACACCCATTCAAGCTCCACTTCCCAAGCCTGTGCAGCAGAGATTGGAGAGGAATGCAGCATATGACAAATCAAAGGAAGAGTTAAGAAAGTGGGAGCCAATCGTAAAGAGGAATAGGGAGGCGCCTACCATATACTTTGACGATGATAGGGATCTAGGGCCTTCATCCGTGGGAACAATAGCATCTGAATTTGAACCTCGAACTGAGTTCGAGAAGAAAATGGCTTCATTACTTTATGATGACGAGGTTATGGATGCTCACAGAAAAGATGGTTCAAGGCTCCTTGAATTAAATAAGGTATCTGCTGAAGATGAAAAGGACCGCCAGAATCGAATCGCCAAAATGCGCAGCCTTCTGTTCCGGCATGAGTTGAAGTCAAAACACATAAAAAACATCAAATCCAAAACCTATCATCGTTTGTTGAAGAAAGATAAATTGAAGGCGAACTCTTTACAAAGTCAAATGGATCCAGAAGCTTCTAAAGAACTTGCAATGAAGCAAGAGTATGAACGAGCAAAGGAACGGATGACTCTGAGGCACAAAGGCAGCTCTAAGTGGGCAAAGCGCATAAAAGAGCGTGGTCTAGATGTCCAAGATGATGGTACTCGAGCTGCTATAGCTGAACAGCAGCACCTTCATGCTCAGCTGACAAGAAAAATGAATTCGATGAAAGATAGTGACACCAGCAGTAATGACACTAGTGATGATGATGATGTGGATGTTAGCTCAGACGACGAGGGTAGGGCATCAAAGTTGTTAGAAAAAGCAAAGGAGAAGACACTTAAACTGTTAAATGAAGATAATGAAGTTCCAAACTCAGGAGTGCTTAGTTTACCTTTCATGGAACGTGCATCTAAAAAAAAAAATGAGGCAGCTGCTGGAGAAGCTAAACTTGCTCTTCAAGAGTATGAATCATGGGCAAATCAATTGGGGGATTCAGGTGGGGGCAAAAATCCGAAAGTAGGCCGTCCGAGTGGGAGAATGGTCTTCAATGGAAAGGAGGCTCCTGAATCAAGTAAGAAGACTAAATCAGATAACAAGAATAGATCAGATAATTATTACGGTGATAGTGATAGTGAAGACGACTTGGAACCCATCAGTATTTCCCCTGTTGGGAAATACAGAAGTAATGAATTAATGAAGGATGCCAACAGTTCTCCTGTTTTACTTCATGAAGATTCTGAAAATCATAAGGATTCTTTTTTCAAGCAAAACACTGATGGTAGCGTGCAAGACCCAGGTCCCCAGGCAACACATGAAGTTTCTATTTTTGCTTCAGGCACATGGAGAAAGATGAAGAATGGAAATGACGCAAATGCCAATGCCAATGCCAAGAAGTCTCCTGCACCCCTGCAATCAGTTGAGCTTGATCAGAATGTGCAAGAACCTGTAAAGGATTTGGATGAGGACAGTGATGCAGATAGTGAGGAACAAATGGTAGATGGGGTTCTTTCAGCGGTACCAGAACCATCATATGAACTTCCTTCCCAAGAAGAGCTAATTCGTCAAGCCTTTGCAGCAGATAATGTGGAGGATGAGTTCAAGAAAACAAAAGAGGATATTTTGAACGAGGAAAATCCCGAACCTGAAAAGCCTGTATTACTCCCTGGGTGGGGTCAGTGGACTAACGTACAGAAAAAGAAAGGAATACCTTCTTATATGCAGATAGAACATGATAGTGCCAAGAAGAAGAGGGAAGAAACCCTTAAACAAAGAAAGGATGCAAATCTCAAACATGTTATTATCTCTGAGAAGCCGCCTAATAAAAAGTCTCAGAACCTTTTCACACAATCATTGCCCTACCCTTTCACGTCCAAAGAAGTTTTTGAACAGAGCATCCGCATGCCCCTTGGTCCTGAATTCAACCCTGCAAGTATGGTTGGAGCTCTTAATCGACCTGAAGTGGTAAAGAAATCTGGTGTAATTATAAAACCCATCGAGTTTGAGGAGGTGAATGCTTATGAAAAATTAGAGGGGCACAAAAAGAGTGGACAGAAACACAAGAAGAACAGCAAGAGTAAAAGCAATAGCAATAGCGGCAAAAATATGACAAAGATGAAGACTAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF_CA GUB_WAK_bind Pkinase WAK

Protein Analysis

900

Amino Acids

101.84

Weight (kDa)

5.83

Isoelectric Point (pI)

49.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Utp14 PF04615 146 - 859 9.1e-170 Utp14 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21210 AT1G21230 AT1G21240 AT1G21240 AT1G21245 AT1G21250 AT1G21270 AT1G22720 AT3G25490
fragaria_vesca FvH4_2g35051 FvH4_2g35051 FvH4_2g35060 FvH4_3g10281 FvH4_3g10285 FvH4_3g10286 FvH4_3g10311 FvH4_3g10311 FvH4_3g10320 FvH4_3g10332 FvH4_3g10340 FvH4_3g10350 FvH4_3g10360 FvH4_3g10390 FvH4_3g10412 FvH4_3g10413 FvH4_3g10461 FvH4_3g10462 FvH4_3g10470 FvH4_3g10510 FvH4_3g10520 FvH4_3g10530 FvH4_3g10550 FvH4_3g10560 FvH4_3g10611 FvH4_3g11730 FvH4_6g53140 FvH4_6g53142 FvH4_7g31880 FvH4_7g31910 FvH4_7g31920
malus_domestica MD05G1269900.v1.1 MD05G1270000.v1.1 MD05G1270200.v1.1 MD05G1270400.v1.1 MD05G1270500.v1.1 MD05G1270600.v1.1 MD05G1270800.v1.1 MD05G1270900.v1.1 MD07G1183800.v1.1 MD08G1107600.v1.1 MD10G1248500.v1.1 MD10G1249200.v1.1 MD10G1249500.v1.1 MD10G1250000.v1.1 MD10G1250500.v1.1 MD10G1250900.v1.1 MD10G1251200.v1.1 MD10G1251400.v1.1 MD13G1004600.v1.1 MD15G1088400.v1.1
prunus_persica Prupe.1G442400_v2.0.a1 Prupe.1G442500_v2.0.a1 Prupe.2G223400_v2.0.a1 Prupe.4G091900_v2.0.a1 Prupe.4G093200_v2.0.a1 Prupe.4G093300_v2.0.a1 Prupe.4G093400_v2.0.a1 Prupe.4G093500_v2.0.a1 Prupe.4G094100_v2.0.a1 Prupe.4G094200_v2.0.a1 Prupe.4G094300_v2.0.a1 Prupe.6G358300_v2.0.a1
pyrus_communis pycom08g08990 pycom10g20660 pycom10g20670 pycom10g20680 pycom10g20710 pycom10g20740 pycom13g00400 pycom15g08290
rosa_chinensis RchiOBHm_Chr1g0326861 RchiOBHm_Chr1g0326871 RchiOBHm_Chr1g0331391 RchiOBHm_Chr1g0336911 RchiOBHm_Chr1g0364001 RchiOBHm_Chr2g0174541 RchiOBHm_Chr2g0175041 RchiOBHm_Chr2g0175101 RchiOBHm_Chr2g0175121 RchiOBHm_Chr3g0486331 RchiOBHm_Chr5g0015581 RchiOBHm_Chr5g0016551 RchiOBHm_Chr5g0016561 RchiOBHm_Chr5g0016581 RchiOBHm_Chr5g0016601 RchiOBHm_Chr5g0016611 RchiOBHm_Chr5g0016641 RchiOBHm_Chr5g0016661 RchiOBHm_Chr5g0016691 RchiOBHm_Chr5g0016701 RchiOBHm_Chr5g0016731 RchiOBHm_Chr5g0016741 RchiOBHm_Chr5g0016811 RchiOBHm_Chr5g0016841 RchiOBHm_Chr5g0016871 RchiOBHm_Chr5g0016881 RchiOBHm_Chr5g0016931 RchiOBHm_Chr5g0017021 RchiOBHm_Chr5g0017041 RchiOBHm_Chr5g0017071 RchiOBHm_Chr5g0017091 RchiOBHm_Chr5g0017121 RchiOBHm_Chr5g0017141 RchiOBHm_Chr5g0017291 RchiOBHm_Chr5g0017321 RchiOBHm_Chr5g0017401 RchiOBHm_Chr5g0019001 RchiOBHm_Chr5g0019071 RchiOBHm_Chr5g0028941 RchiOBHm_Chr5g0052141 RchiOBHm_Chr5g0054721 RchiOBHm_Chr5g0054801 RchiOBHm_Chr5g0054931 RchiOBHm_Chr5g0054941 RchiOBHm_Chr6g0259291 RchiOBHm_Chr6g0292991 RchiOBHm_Chr6g0306911
rosa_laevigata RLG00000009465 RLG00000009468 RLG00000010755 RLG00000011976 RLG00000014541 RLG00000014542 RLG00000015169 RLG00000022308 RLG00000027529 RLG00000029324 RLG00000030244 RLG00000030245 RLG00000032210 RLG00000032211 RLG00000032257 RLG00000032259 RLG00000032260 RLG00000032261 RLG00000032262 RLG00000032268 RLG00000032269 RLG00000032272 RLG00000032282 RLG00000032283 RLG00000032308 RLG00000032413 RLG00000033129 RLG00000034945
rosa_multiflora Rmu_co8241929.1_g000001 Rmu_co8269227.1_g000001 Rmu_co8461259.1_g000001 Rmu_co8495385.1_g000001 Rmu_sc0000028.1_g000003 Rmu_sc0000083.1_g000009 Rmu_sc0000083.1_g000010 Rmu_sc0000083.1_g000031 Rmu_sc0000083.1_g000043 Rmu_sc0000083.1_g000046 Rmu_sc0000083.1_g000047 Rmu_sc0000563.1_g000024 Rmu_sc0000842.1_g000012 Rmu_sc0001235.1_g000001 Rmu_sc0001235.1_g000005 Rmu_sc0001235.1_g000009 Rmu_sc0001235.1_g000015 Rmu_sc0001235.1_g000016 Rmu_sc0001235.1_g000017 Rmu_sc0001473.1_g000036 Rmu_sc0001607.1_g000016 Rmu_sc0001788.1_g000006 Rmu_sc0002018.1_g000008 Rmu_sc0002028.1_g000001 Rmu_sc0002680.1_g000011 Rmu_sc0002798.1_g000023 Rmu_sc0002886.1_g000023 Rmu_sc0002989.1_g000012 Rmu_sc0003064.1_g000009 Rmu_sc0003382.1_g000006 Rmu_sc0003630.1_g000002 Rmu_sc0003630.1_g000047 Rmu_sc0003630.1_g000066 Rmu_sc0003630.1_g000067 Rmu_sc0003649.1_g000010 Rmu_sc0003688.1_g000005 Rmu_sc0003920.1_g000007 Rmu_sc0004765.1_g000007 Rmu_sc0004765.1_g000008 Rmu_sc0004920.1_g000011 Rmu_sc0005223.1_g000008 Rmu_sc0005223.1_g000010 Rmu_sc0005223.1_g000015 Rmu_sc0005223.1_g000016 Rmu_sc0005319.1_g000014 Rmu_sc0005319.1_g000015 Rmu_sc0005319.1_g000018 Rmu_sc0005319.1_g000019 Rmu_sc0005319.1_g000025 Rmu_sc0005418.1_g000007 Rmu_sc0005418.1_g000012 Rmu_sc0006106.1_g000011 Rmu_sc0006914.1_g000012 Rmu_sc0007023.1_g000001 Rmu_sc0007662.1_g000001 Rmu_sc0007920.1_g000002 Rmu_sc0007920.1_g000013 Rmu_sc0008053.1_g000016 Rmu_sc0008053.1_g000017 Rmu_sc0008053.1_g000018 Rmu_sc0008053.1_g000023 Rmu_sc0008053.1_g000050 Rmu_sc0009042.1_g000007 Rmu_sc0009042.1_g000008 Rmu_sc0010642.1_g000008 Rmu_sc0011109.1_g000002 Rmu_sc0011314.1_g000006 Rmu_sc0011974.1_g000006 Rmu_sc0012179.1_g000009 Rmu_sc0015169.1_g000001 Rmu_sc0016368.1_g000002 Rmu_sc0022659.1_g000001 Rmu_sc0027371.1_g000001 Rmu_sc0028213.1_g000001 Rmu_sc0034526.1_g000001 Rmu_sc0039366.1_g000002 Rmu_ssc0000050.1_g000084 Rmu_ssc0000050.1_g000088 Rmu_ssc0000167.1_g000017
rosa_roxburghii Rroxscaffold_1G00011340 Rroxscaffold_1G00025710 Rroxscaffold_1G00025870 Rroxscaffold_1G00026070 Rroxscaffold_1G00026170 Rroxscaffold_1G00026200 Rroxscaffold_1G00026270 Rroxscaffold_1G00050950 Rroxscaffold_1G00058900 Rroxscaffold_1G00060040 Rroxscaffold_1G00060060 Rroxscaffold_1G00060070 Rroxscaffold_1G00060080 Rroxscaffold_1G00060110 Rroxscaffold_1G00060120 Rroxscaffold_1G00060140 Rroxscaffold_1G00060170 Rroxscaffold_1G00060210 Rroxscaffold_1G00060220 Rroxscaffold_1G00060240 Rroxscaffold_1G00060250 Rroxscaffold_1G00060260 Rroxscaffold_1G00060300 Rroxscaffold_1G00060320 Rroxscaffold_1G00060330 Rroxscaffold_1G00060900 Rroxscaffold_1G00060920 Rroxscaffold_1G00073980 Rroxscaffold_2G00077560 Rroxscaffold_2G00077580 Rroxscaffold_2G00146990 Rroxscaffold_3G00236200 Rroxscaffold_3G00236210 Rroxscaffold_3G00236220 Rroxscaffold_4G00292760 Rroxscaffold_4G00315200 Rroxscaffold_4G00315240 Rroxscaffold_4G00326240 Rroxscaffold_4G00328980 Rroxscaffold_5G00341890 Rroxscaffold_7G00161350 Rroxscaffold_7G00174490 Rroxscaffold_7G00206550
rosa_rugosa Rorug01G0093000 Rorug01G0093100 Rorug01G0128800 Rorug01G0312000 Rorug01G0312000 Rorug02G0581100 Rorug03G0224600 Rorug05G0028500 Rorug05G0032700 Rorug05G0032800 Rorug05G0032900 Rorug05G0032900 Rorug05G0033000 Rorug05G0033100 Rorug05G0033300 Rorug05G0033400 Rorug05G0033900 Rorug05G0034900 Rorug05G0035000 Rorug05G0046900 Rorug05G0047500 Rorug05G0047500 Rorug05G0111600 Rorug05G0287900 Rorug05G0288700 Rorug05G0586900 Rorug06G0231700 Rorug06G0354300 Rorug06G0354400
rosa_samantha Rh1DG122200 Rh2DG686800 Rh5BG136600 Rh6AG344500 Rh6BG351900 Rh6CG359000 Rh6CG359100 Rh6CG387600 Rh6CG480500 Rh6DG344600 Rh6DG467400
rosa_wichuraiana Rw0G010250 Rw0G014820 Rw0G021400 Rw0G023100 Rw1G009450 Rw1G012100 Rw1G012180 Rw1G028360 Rw2G054260 Rw3G024370 Rw3G024420 Rw3G024470 Rw5G010850 Rw5G011010 Rw5G011020 Rw5G011050 Rw5G011080 Rw5G011120 Rw5G012300 Rw5G018470 Rw5G033830 Rw6G008930 Rw6G030070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2561
Acc16I TGCGCA 1 cut(s) 980
Acc65I GGTACC 1 cut(s) 2092
AccB1I GGYRCC 2 cut(s) 754, 2092
AccI GTMKAC 1 cut(s) 40
AclWI GGATC 4 cut(s) 272, 792, 1094, 1107
AcoI YGGCCR 1 cut(s) 432
AcsI RAATTY 3 cut(s) 821, 1294, 2492
AcuI CTGAAG 3 cut(s) 960, 1893, 2553
AcyI GRCGYC 1 cut(s) 755
AfaI GTAC 4 cut(s) 612, 1237, 2094, 2265
AfiI CCNNNNNNNGG 6 cut(s) 575, 1451, 1595, 1746, 2245, 2246
AflII CTTAAG 1 cut(s) 599
AflIII ACRYGT 1 cut(s) 2368
AhlI ACTAGT 1 cut(s) 1331
AjiI CACGTC 1 cut(s) 2445
AjnI CCWGG 5 cut(s) 213, 356, 1870, 1878, 2239
AloI GAACNNNNNNTCC 4 cut(s) 2156, 2188, 2454, 2486
Alw21I GWGCWC 3 cut(s) 147, 544, 2521
Alw26I GTCTC 6 cut(s) 2, 21, 431, 549, 1977, 2409
AlwI GGATC 4 cut(s) 272, 792, 1094, 1107
AlwNI CAGNNNCTG 2 cut(s) 220, 1511
Ama87I CYCGRG 2 cut(s) 494, 1239
AoxI GGCC 6 cut(s) 112, 211, 354, 432, 791, 1597
ApoI RAATTY 3 cut(s) 821, 1294, 2492
AseI ATTAAT 1 cut(s) 1769
Asp700I GAANNNNTTC 2 cut(s) 2284, 2414
Asp718I GGTACC 1 cut(s) 2092
AspLEI GCGC 4 cut(s) 92, 757, 981, 1200
AspS9I GGNCC 8 cut(s) 113, 211, 217, 565, 791, 952, 1874, 2486
AsuHPI GGTGA 3 cut(s) 545, 1709, 2596
AvaI CYCGRG 2 cut(s) 494, 1239
AvaII GGWCC 5 cut(s) 217, 565, 952, 1874, 2486
AxyI CCTNAGG 1 cut(s) 309
BaeGI GKGCMC 1 cut(s) 2613
BamHI GGATCC 1 cut(s) 1099
BanI GGYRCC 2 cut(s) 754, 2092
BanII GRGCYC 2 cut(s) 147, 2521
BauI CACGAG 1 cut(s) 516
BbsI GAAGAC 5 cut(s) 1415, 1612, 1661, 1719, 2699
Bbv12I GWGCWC 3 cut(s) 147, 544, 2521
BccI CCATC 8 cut(s) 401, 896, 1226, 1736, 1847, 2069, 2110, 2576
BceAI ACGGC 1 cut(s) 1584
BciT130I CCWGG 5 cut(s) 215, 358, 1872, 1880, 2241
BclI TGATCA 1 cut(s) 2002
BcoDI GTCTC 6 cut(s) 2, 21, 431, 549, 1977, 2409
BcuI ACTAGT 1 cut(s) 1331
BfaI CTAG 4 cut(s) 162, 788, 1217, 1332
BfmI CTRYAG 1 cut(s) 1248
BfoI RGCGCY 2 cut(s) 93, 758
BfrI CTTAAG 1 cut(s) 599
BglI GCCNNNNNGGC 1 cut(s) 98
BlpI GCTNAGC 1 cut(s) 1275
Bme1390I CCNGG 5 cut(s) 215, 358, 1872, 1880, 2241
Bme18I GGWCC 5 cut(s) 217, 565, 952, 1874, 2486
BmeT110I CYCGRG 2 cut(s) 494, 1239
BmgBI CACGTC 1 cut(s) 2445
BmgT120I GGNCC 8 cut(s) 113, 211, 217, 565, 791, 952, 1874, 2486
BmrFI CCNGG 5 cut(s) 215, 358, 1872, 1880, 2241
BmsI GCATC 8 cut(s) 824, 877, 1388, 1493, 1768, 2038, 2344, 2478
BoxI GACNNNNGTC 1 cut(s) 1872
BpiI GAAGAC 5 cut(s) 1415, 1612, 1661, 1719, 2699
BplI GAGNNNNNCTC 2 cut(s) 176, 208
BpmI CTGGAG 1 cut(s) 1533
Bpu1102I GCTNAGC 1 cut(s) 1275
BsaAI YACGTR 1 cut(s) 614
BsaBI GATNNNNATC 2 cut(s) 783, 2358
BsaHI GRCGYC 1 cut(s) 755
BsaI GGTCTC 2 cut(s) 2, 549
BsaJI CCNNGG 7 cut(s) 574, 801, 1870, 1878, 2239, 2240, 2482
BsaXI ACNNNNNCTCC 4 cut(s) 239, 269, 2156, 2186
Bsc4I CCNNNNNNNGG 6 cut(s) 575, 1451, 1595, 1746, 2245, 2246
Bse118I RCCGGY 2 cut(s) 270, 430
Bse21I CCTNAGG 1 cut(s) 309
Bse3DI GCAATG 2 cut(s) 1131, 2426
Bse8I GATNNNNATC 2 cut(s) 783, 2358
BseBI CCWGG 5 cut(s) 215, 358, 1872, 1880, 2241
BseDI CCNNGG 7 cut(s) 574, 801, 1870, 1878, 2239, 2240, 2482
BseJI GATNNNNATC 2 cut(s) 783, 2358
BseLI CCNNNNNNNGG 6 cut(s) 575, 1451, 1595, 1746, 2245, 2246
BseMI GCAATG 2 cut(s) 1131, 2426
BseMII CTCAG 8 cut(s) 300, 828, 1157, 1289, 1374, 1464, 2373, 2420
BseRI GAGGAG 4 cut(s) 263, 266, 466, 2594
BseSI GKGCMC 1 cut(s) 2613
BseX3I CGGCCG 1 cut(s) 432
BsgI GTGCAG 2 cut(s) 686, 1962
Bsh1285I CGRYCG 2 cut(s) 151, 435
BshFI GGCC 6 cut(s) 114, 213, 356, 434, 793, 1599
BshNI GGYRCC 2 cut(s) 754, 2092
BsiEI CGRYCG 2 cut(s) 151, 435
BsiHKAI GWGCWC 3 cut(s) 147, 544, 2521
BsiHKCI CYCGRG 2 cut(s) 494, 1239
BsiSI CCGG 4 cut(s) 245, 271, 431, 994
BslFI GGGAC 2 cut(s) 252, 1860
BslI CCNNNNNNNGG 6 cut(s) 575, 1451, 1595, 1746, 2245, 2246
BsmAI GTCTC 6 cut(s) 2, 21, 431, 549, 1977, 2409
BsmBI CGTCTC 1 cut(s) 21
BsmFI GGGAC 2 cut(s) 252, 1860
BsmI GAATGC 2 cut(s) 691, 2593
BsnI GGCC 6 cut(s) 114, 213, 356, 434, 793, 1599
Bso31I GGTCTC 2 cut(s) 2, 549
BsoBI CYCGRG 2 cut(s) 494, 1239
Bsp1286I GDGCHC 4 cut(s) 147, 544, 2521, 2613
Bsp143I GATC 5 cut(s) 277, 784, 1099, 1679, 2002
Bsp1720I GCTNAGC 1 cut(s) 1275
BspANI GGCC 6 cut(s) 114, 213, 356, 434, 793, 1599
BspCNI CTCAG 8 cut(s) 301, 829, 1158, 1288, 1373, 1463, 2374, 2419
BspHI TCATGA 1 cut(s) 1804
BspPI GGATC 4 cut(s) 272, 792, 1094, 1107
BspQI GCTCTTC 2 cut(s) 1536, 2121
BspT107I GGYRCC 2 cut(s) 754, 2092
BspTI CTTAAG 1 cut(s) 599
BspTNI GGTCTC 2 cut(s) 2, 549
BsrDI GCAATG 2 cut(s) 1131, 2426
BsrFI RCCGGY 2 cut(s) 270, 430
BssAI RCCGGY 2 cut(s) 270, 430
BssECI CCNNGG 7 cut(s) 574, 801, 1870, 1878, 2239, 2240, 2482
BssMI GATC 5 cut(s) 277, 784, 1099, 1679, 2002
BssNI GRCGYC 1 cut(s) 755
BssSI CACGAG 1 cut(s) 516
BssT1I CCWWGG 2 cut(s) 574, 2482
Bst2BI CACGAG 1 cut(s) 516
Bst2UI CCWGG 5 cut(s) 215, 358, 1872, 1880, 2241
Bst4CI ACNGT 5 cut(s) 365, 1422, 1697, 1787, 2045
Bst6I CTCTTC 8 cut(s) 177, 195, 228, 599, 705, 1536, 2121, 2318
BstACI GRCGYC 1 cut(s) 755
BstAFI CTTAAG 1 cut(s) 599
BstBAI YACGTR 1 cut(s) 614
BstC8I GCNNGC 4 cut(s) 122, 432, 1862, 2477
BstDSI CCRYGG 1 cut(s) 801
BstENI CCTNNNNNAGG 1 cut(s) 573
BstH2I RGCGCY 2 cut(s) 93, 758
BstHHI GCGC 4 cut(s) 92, 757, 981, 1200
BstKTI GATC 5 cut(s) 280, 787, 1102, 1682, 2005
BstMAI GTCTC 6 cut(s) 2, 21, 431, 549, 1977, 2409
BstMBI GATC 5 cut(s) 277, 784, 1099, 1679, 2002
BstMCI CGRYCG 2 cut(s) 151, 435
BstMWI GCNNNNNNNGC 9 cut(s) 81, 98, 142, 667, 1250, 1517, 1526, 2150, 2670
BstNI CCWGG 5 cut(s) 215, 358, 1872, 1880, 2241
BstNSI RCATGY 2 cut(s) 2372, 2479
BstPAI GACNNNNGTC 1 cut(s) 1872
BstSCI CCNGG 5 cut(s) 213, 356, 1870, 1878, 2239
BstSFI CTRYAG 1 cut(s) 1248
BstSLI GKGCMC 1 cut(s) 2613
BstSNI TACGTA 1 cut(s) 614
BstV2I GAAGAC 5 cut(s) 1415, 1612, 1661, 1719, 2699
BstX2I RGATCY 2 cut(s) 784, 1099
BstXI CCANNNNNNTGG 1 cut(s) 1232
BstYI RGATCY 2 cut(s) 784, 1099
BstZI CGGCCG 1 cut(s) 432
Bsu36I CCTNAGG 1 cut(s) 309
BsuRI GGCC 6 cut(s) 114, 213, 356, 434, 793, 1599
BtgI CCRYGG 1 cut(s) 801
BtrI CACGTC 1 cut(s) 2445
BtsIMutI CAGTG 3 cut(s) 1848, 2050, 2258
Cac8I GCNNGC 4 cut(s) 122, 432, 1862, 2477
CaiI CAGNNNCTG 2 cut(s) 220, 1511
CciI TCATGA 1 cut(s) 1804
CfoI GCGC 4 cut(s) 92, 757, 981, 1200
Cfr10I RCCGGY 2 cut(s) 270, 430
Cfr13I GGNCC 8 cut(s) 113, 211, 217, 565, 791, 952, 1874, 2486
CseI GACGC 1 cut(s) 1952
Csp6I GTAC 4 cut(s) 611, 1236, 2093, 2264
CspCI CAANNNNNGTGG 2 cut(s) 2602, 2637
CviQI GTAC 4 cut(s) 611, 1236, 2093, 2264
DinI GGCGCC 1 cut(s) 756
DpnI GATC 5 cut(s) 279, 786, 1101, 1681, 2004
DpnII GATC 5 cut(s) 277, 784, 1099, 1679, 2002
EaeI YGGCCR 1 cut(s) 432
EagI CGGCCG 1 cut(s) 432
Eam1104I CTCTTC 8 cut(s) 177, 195, 228, 599, 705, 1536, 2121, 2318
EarI CTCTTC 8 cut(s) 177, 195, 228, 599, 705, 1536, 2121, 2318
EciI GGCGGA 1 cut(s) 395
Ecl136II GAGCTC 2 cut(s) 145, 2519
EclXI CGGCCG 1 cut(s) 432
Eco105I TACGTA 1 cut(s) 614
Eco130I CCWWGG 2 cut(s) 574, 2482
Eco24I GRGCYC 2 cut(s) 147, 2521
Eco31I GGTCTC 2 cut(s) 2, 549
Eco47I GGWCC 5 cut(s) 217, 565, 952, 1874, 2486
Eco52I CGGCCG 1 cut(s) 432
Eco53kI GAGCTC 2 cut(s) 145, 2519
Eco57I CTGAAG 3 cut(s) 960, 1893, 2553
Eco81I CCTNAGG 1 cut(s) 309
Eco88I CYCGRG 2 cut(s) 494, 1239
EcoICRI GAGCTC 2 cut(s) 145, 2519
EcoNI CCTNNNNNAGG 1 cut(s) 573
EcoO109I RGGNCCY 4 cut(s) 113, 217, 791, 1874
EcoRI GAATTC 2 cut(s) 1294, 2492
EcoRII CCWGG 5 cut(s) 213, 356, 1870, 1878, 2239
EcoT14I CCWWGG 2 cut(s) 574, 2482
EcoT38I GRGCYC 2 cut(s) 147, 2521
EgeI GGCGCC 1 cut(s) 756
EheI GGCGCC 1 cut(s) 756
ErhI CCWWGG 2 cut(s) 574, 2482
Esp3I CGTCTC 1 cut(s) 21
FalI AAGNNNNNCTT 8 cut(s) 528, 560, 636, 668, 1509, 1541, 2322, 2354
FaqI GGGAC 2 cut(s) 252, 1860
FauNDI CATATG 2 cut(s) 694, 2107
FbaI TGATCA 1 cut(s) 2002
FblI GTMKAC 1 cut(s) 40
FriOI GRGCYC 2 cut(s) 147, 2521
FspBI CTAG 4 cut(s) 162, 788, 1217, 1332
FspI TGCGCA 1 cut(s) 980
GlaI GCGC 4 cut(s) 91, 756, 980, 1199
GsuI CTGGAG 1 cut(s) 1533
HaeII RGCGCY 2 cut(s) 93, 758
HaeIII GGCC 6 cut(s) 114, 213, 356, 434, 793, 1599
HapII CCGG 4 cut(s) 245, 271, 431, 994
HgaI GACGC 1 cut(s) 1952
HhaI GCGC 4 cut(s) 92, 757, 981, 1200
Hin1I GRCGYC 1 cut(s) 755
Hin6I GCGC 4 cut(s) 90, 755, 979, 1198
HinP1I GCGC 4 cut(s) 90, 755, 979, 1198
HindIII AAGCTT 1 cut(s) 1107
HpaII CCGG 4 cut(s) 245, 271, 431, 994
HphI GGTGA 3 cut(s) 545, 1709, 2596
Hpy166II GTNNAC 4 cut(s) 41, 1466, 2256, 2624
Hpy8I GTNNAC 4 cut(s) 41, 1466, 2256, 2624
Hpy99I CGWCG 4 cut(s) 35, 182, 287, 1370
HpyCH4III ACNGT 5 cut(s) 365, 1422, 1697, 1787, 2045
HpyCH4IV ACGT 4 cut(s) 613, 1480, 2262, 2444
HpyF10VI GCNNNNNNNGC 9 cut(s) 81, 98, 142, 667, 1250, 1517, 1526, 2150, 2670
HpySE526I ACGT 4 cut(s) 613, 1480, 2262, 2444
Hsp92I GRCGYC 1 cut(s) 755
HspAI GCGC 4 cut(s) 90, 755, 979, 1198
KasI GGCGCC 1 cut(s) 754
KpnI GGTACC 1 cut(s) 2096
KroI GCCGGC 1 cut(s) 430
KroNI GCCGGC 1 cut(s) 432
Ksp22I TGATCA 1 cut(s) 2002
Kzo9I GATC 5 cut(s) 277, 784, 1099, 1679, 2002
LguI GCTCTTC 2 cut(s) 1536, 2121
LmnI GCTCC 9 cut(s) 48, 142, 197, 539, 652, 727, 918, 1642, 2516
LweI GCATC 8 cut(s) 824, 877, 1388, 1493, 1768, 2038, 2344, 2478
MaeI CTAG 4 cut(s) 162, 788, 1217, 1332
MaeII ACGT 4 cut(s) 613, 1480, 2262, 2444
MaeIII GTNAC 1 cut(s) 1310
MalI GATC 5 cut(s) 279, 786, 1101, 1681, 2004
MboI GATC 5 cut(s) 277, 784, 1099, 1679, 2002
MfeI CAATTG 1 cut(s) 1559
MflI RGATCY 2 cut(s) 784, 1099
MhlI GDGCHC 4 cut(s) 147, 544, 2521, 2613
Mly113I GGCGCC 1 cut(s) 755
MlyI GAGTC 4 cut(s) 167, 263, 506, 1156
MmeI TCCRAC 4 cut(s) 201, 462, 507, 2494
MroNI GCCGGC 1 cut(s) 430
MroXI GAANNNNTTC 2 cut(s) 2284, 2414
MslI CAYNNNNRTG 3 cut(s) 1269, 2310, 2474
MspA1I CMGCKG 4 cut(s) 46, 1279, 1508, 2090
MspCI CTTAAG 1 cut(s) 599
MspI CCGG 4 cut(s) 245, 271, 431, 994
MspR9I CCNGG 5 cut(s) 215, 358, 1872, 1880, 2241
MunI CAATTG 1 cut(s) 1559
Mva1269I GAATGC 2 cut(s) 691, 2593
MvaI CCWGG 5 cut(s) 215, 358, 1872, 1880, 2241
MwoI GCNNNNNNNGC 9 cut(s) 81, 98, 142, 667, 1250, 1517, 1526, 2150, 2670
NaeI GCCGGC 1 cut(s) 432
NarI GGCGCC 1 cut(s) 755
NdeI CATATG 2 cut(s) 694, 2107
NdeII GATC 5 cut(s) 277, 784, 1099, 1679, 2002
NgoMIV GCCGGC 1 cut(s) 430
NmeAIII GCCGAG 1 cut(s) 460
NmuCI GTSAC 1 cut(s) 1310
NsbI TGCGCA 1 cut(s) 980
NspI RCATGY 2 cut(s) 2372, 2479
PaeI GCATGC 1 cut(s) 2479
PaeR7I CTCGAG 1 cut(s) 1239
PagI TCATGA 1 cut(s) 1804
PasI CCCWGGG 1 cut(s) 2240
PciI ACATGT 1 cut(s) 2368
PciSI GCTCTTC 2 cut(s) 1536, 2121
PcsI WCGNNNNNNNCGW 2 cut(s) 282, 291
PctI GAATGC 2 cut(s) 691, 2593
PdiI GCCGGC 1 cut(s) 432
PdmI GAANNNNTTC 2 cut(s) 2284, 2414
PfeI GAWTC 8 cut(s) 417, 961, 966, 1544, 1568, 1643, 1811, 1829
PleI GAGTC 4 cut(s) 167, 262, 505, 1156
PluTI GGCGCC 1 cut(s) 758
PpsI GAGTC 4 cut(s) 167, 262, 505, 1156
Ppu21I YACGTR 1 cut(s) 614
PpuMI RGGWCCY 2 cut(s) 217, 1874
PscI ACATGT 1 cut(s) 2368
PshAI GACNNNNGTC 1 cut(s) 1872
PshBI ATTAAT 1 cut(s) 1769
PsiI TTATAA 1 cut(s) 2561
Psp124BI GAGCTC 2 cut(s) 147, 2521
Psp5II RGGWCCY 2 cut(s) 217, 1874
Psp6I CCWGG 5 cut(s) 213, 356, 1870, 1878, 2239
PspGI CCWGG 5 cut(s) 213, 356, 1870, 1878, 2239
PspPI GGNCC 8 cut(s) 113, 211, 217, 565, 791, 952, 1874, 2486
PspPPI RGGWCCY 2 cut(s) 217, 1874
PspXI VCTCGAGB 1 cut(s) 1239
PsrI GAACNNNNNNTAC 2 cut(s) 2064, 2096
PstNI CAGNNNCTG 2 cut(s) 220, 1511
PsuI RGATCY 2 cut(s) 784, 1099
PvuII CAGCTG 2 cut(s) 1279, 1508
RsaI GTAC 4 cut(s) 612, 1237, 2094, 2265
RsaNI GTAC 4 cut(s) 611, 1236, 2093, 2264
RseI CAYNNNNRTG 3 cut(s) 1269, 2310, 2474
SacI GAGCTC 2 cut(s) 147, 2521
SapI GCTCTTC 2 cut(s) 1536, 2121
Sau3AI GATC 5 cut(s) 277, 784, 1099, 1679, 2002
Sau96I GGNCC 8 cut(s) 113, 211, 217, 565, 791, 952, 1874, 2486
SchI GAGTC 4 cut(s) 167, 263, 506, 1156
ScrFI CCNGG 5 cut(s) 215, 358, 1872, 1880, 2241
SduI GDGCHC 4 cut(s) 147, 544, 2521, 2613
SfaNI GCATC 8 cut(s) 824, 877, 1388, 1493, 1768, 2038, 2344, 2478
SfcI CTRYAG 1 cut(s) 1248
SfoI GGCGCC 1 cut(s) 756
Sfr274I CTCGAG 1 cut(s) 1239
SinI GGWCC 5 cut(s) 217, 565, 952, 1874, 2486
SlaI CTCGAG 1 cut(s) 1239
SmiMI CAYNNNNRTG 3 cut(s) 1269, 2310, 2474
SmlI CTYRAG 2 cut(s) 599, 1239
SmoI CTYRAG 2 cut(s) 599, 1239
SnaBI TACGTA 1 cut(s) 614
SpeI ACTAGT 1 cut(s) 1331
SphI GCATGC 1 cut(s) 2479
SspDI GGCGCC 1 cut(s) 754
SspMI CTAG 4 cut(s) 162, 788, 1217, 1332
SstI GAGCTC 2 cut(s) 147, 2521
StyD4I CCNGG 5 cut(s) 213, 356, 1870, 1878, 2239
StyI CCWWGG 2 cut(s) 574, 2482
TaaI ACNGT 5 cut(s) 365, 1422, 1697, 1787, 2045
TaiI ACGT 4 cut(s) 616, 1483, 2265, 2447
TauI GCSGC 2 cut(s) 2392, 2673
TfiI GAWTC 8 cut(s) 417, 961, 966, 1544, 1568, 1643, 1811, 1829
TscAI CASTG 3 cut(s) 1855, 2050, 2258
TseFI GTSAC 1 cut(s) 1310
Tsp45I GTSAC 1 cut(s) 1310
TspGWI ACGGA 3 cut(s) 48, 790, 1171
TspRI CASTG 3 cut(s) 1855, 2050, 2258
Vha464I CTTAAG 1 cut(s) 599
VpaK11BI GGWCC 5 cut(s) 217, 565, 952, 1874, 2486
VspI ATTAAT 1 cut(s) 1769
XagI CCTNNNNNAGG 1 cut(s) 573
XapI RAATTY 3 cut(s) 821, 1294, 2492
XbaI TCTAGA 1 cut(s) 1216
XceI RCATGY 2 cut(s) 2372, 2479
XcmI CCANNNNNNNNNTGG 1 cut(s) 581
XhoI CTCGAG 1 cut(s) 1239
XmiI GTMKAC 1 cut(s) 40
XmnI GAANNNNTTC 2 cut(s) 2284, 2414
XspI CTAG 4 cut(s) 162, 788, 1217, 1332
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.