RchiOBHm_Chr5g0016881

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000066
Physical Location & Seq
Reverse (-)
11578058 .. 11580827
2770 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29723

Sequence Viewer

Length: 2262 bp
ATGTCGATCATGGCCTTGCAAATCCCATTGGCGGCAGTCCTAATCCTATTATCGGCGGCTAGTACTGCGACAACAACAACTGCTGCTCAAGCCCTGCCTGGCTGCTCTGACAAGTGCGGCAATCTCACAGTTCCATATCCATTTGGCATGAATAGTGATTGTTACCTGCACAAAGAATTCTTTATCAATTGTAGCCATACCACCCGACCACCAACACCTTATTTGATGAATGGGAATCTCATTGTTACTAACATATCCGTAGACGGGGAGTTGCATTTACTCAACTATGTAGGGCGGGATTGTTACAATAAAGAGGACAATCGAACTGAAAGAAGCACAGAACCTGAGCTCTGGTTGGTACCTCCGTACACCATATCCGATACCAAGAACAAGTTCTACGCTGTTGGTTGTGACACTTACGCAATCATGCGAGGCTTCCGAGGGGAAGATGAATTCATTACTGGGTGCATGTCAATATGCAACAGCCTTGCCAGTGTTGATAAAAACTCTTGCTCTGGCTCTGGGTGTTGCCAGACTAACATCCCTAGTGGATTGAACAATCTTACTATGAGTTTGAGTAGCTACAACAATCATTTGGATGTGTGGGACTTTAACCCCTGCAGCTACGCCTTCATTGTACAGCAAGACCAGTTCGCTTTCTCCTTTTCCAGTTTTGAACTATTGAATGACACTGAACGGCTTCCAATGGTCCTTAATTGGGACATTGGGAATGACACAGACTCCTGTCAAGCATGCGGAGCTAATAGCATGTGCAAAAACCGGTCTATCATTGGGTCTGTAGGTTATTTGTGCGAGTGTTTGCCTGGTTATGAAGGAAACCCGTACCACCCAGAAGGTTGCCAAGATATTGACGAGTGCAAGGCTAACCCCTGCGAGAACGGACAGTGCTTAAATATTCCACCTCCAGGAAATTACTCTTGTTCATGTTACGAAGGATACAAACACGACGGCATGAATGACAAGATTTGCATCAAAGACGATTCCAAAAGAGACTCAAAGATCGTTCTCCTGCTTATGATTTCATTGGGTGTAAGTGTAGGCTTCTTGGTTTTATTCCTTGGAATTTCGTGGATAAGTTGGGGAATGAAGAAAAGAGAGTTCATTAAACTCAAAGAAAAGTACTTCAAAGAGAATGGTGGCTTATTGTTACTGCAACAACTCGCTAGTCATGGAAGCTCGATGAAGACAACAAAAATCTTTACTGCTGAAGAACTTGAGAAGGCAACAAACAACTATCATGAGAGTGGAGTCCTTGGAGAAGGAGGTTATGGAACAGTTTACAAAGGTATACTACCGGATGACACAATGGTTGCCATAAAGAAGTCGAAAGGTGCTGCCCTTACCCAGAGTGATCAGTTTGTTAACGAGGTGATTGTCCTTTCTCAAATCAACCACAGAAATGTGGTAAAGCTATTAGGTTGTTGTTTGGAAACAGAAGCGCCTTTACTAGTATACGAATTCATTACGCATGGCACTCTTTATGAGCATATTCATAAAAAAAGATCATCACTCCCATTTGAATTACGAATGAAGATAGCAACTCAAAGCGCGGAAGCGCTGTCCCACTTACACTCCTCAATTTCCACACCAATAATACATCGAGATGTGAAAACATCAAATATTTTGTTAGATGATGATTATACGGCAAAAGTGTCAGATTTTGGAGCTTCCCGATTGGTTCCTTCAGGTCAAACTGATATACAAACTTTAGTGCTTGGGACATTTGGATACCTGGACCCTGAATATCTGCAATCAAACCAACTAACAGAAAAGAGTGATGTTTACAGCTTTGGAGTTGTCCTAGTGGAGCTACTAACAAGCAAAGTGCCGGTTTCTAAAGATAGATGCTTAACAAGCATCTTTCTTGCTTCCATGGAAGAAGGTTGGTTGAATCAAATACTTGATGATGGCATGGTGAATGAGGAAAACATTGAGACGGTAAAGAAAGTGGCCAATCTAGCAAAACGATGCTTGAGGGTAAATGGGGAGGAAAGGCCTACCATGAAAGAAGTTGTGAAAGAGTTAGAGGAAATGAGTGTCATGGCAAAACATCCGTGGGGATTTAATGCTAATTTTTGCAAAGAAGAGACTGAATACTTGCTTGGGTCATTTAATTCAGACGCTTATGTTGTGGGTAATGGAGAAGGTGATTGCAGTTCTAGTGGTCTAACCAGTGGTACGACCACTGCGTATGACGGCATGCAAATCGAACAGTTAATGCCACATGATGGTGGACGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK

Protein Analysis

753

Amino Acids

83.17

Weight (kDa)

5.05

Isoelectric Point (pI)

38.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 35 - 89 1.9e-09 Wall-associated receptor kinase galacturonan-binding
Pkinase PF00069 419 - 680 1.8e-50 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 424 - 682 2.3e-51 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21210 AT1G21230 AT1G21240 AT1G21240 AT1G21245 AT1G21250 AT1G21270 AT1G22720 AT3G25490
fragaria_vesca FvH4_2g35051 FvH4_2g35051 FvH4_2g35060 FvH4_3g10281 FvH4_3g10285 FvH4_3g10286 FvH4_3g10311 FvH4_3g10311 FvH4_3g10320 FvH4_3g10332 FvH4_3g10340 FvH4_3g10350 FvH4_3g10360 FvH4_3g10390 FvH4_3g10412 FvH4_3g10413 FvH4_3g10461 FvH4_3g10462 FvH4_3g10470 FvH4_3g10510 FvH4_3g10520 FvH4_3g10530 FvH4_3g10550 FvH4_3g10560 FvH4_3g10611 FvH4_3g11730 FvH4_6g53140 FvH4_6g53142 FvH4_7g31880 FvH4_7g31910 FvH4_7g31920
malus_domestica MD05G1269900.v1.1 MD05G1270000.v1.1 MD05G1270200.v1.1 MD05G1270400.v1.1 MD05G1270500.v1.1 MD05G1270600.v1.1 MD05G1270800.v1.1 MD05G1270900.v1.1 MD07G1183800.v1.1 MD08G1107600.v1.1 MD10G1248500.v1.1 MD10G1249200.v1.1 MD10G1249500.v1.1 MD10G1250000.v1.1 MD10G1250500.v1.1 MD10G1250900.v1.1 MD10G1251200.v1.1 MD10G1251400.v1.1 MD13G1004600.v1.1 MD15G1088400.v1.1
prunus_persica Prupe.1G442400_v2.0.a1 Prupe.1G442500_v2.0.a1 Prupe.2G223400_v2.0.a1 Prupe.4G091900_v2.0.a1 Prupe.4G093200_v2.0.a1 Prupe.4G093300_v2.0.a1 Prupe.4G093400_v2.0.a1 Prupe.4G093500_v2.0.a1 Prupe.4G094100_v2.0.a1 Prupe.4G094200_v2.0.a1 Prupe.4G094300_v2.0.a1 Prupe.6G358300_v2.0.a1
pyrus_communis pycom08g08990 pycom10g20660 pycom10g20670 pycom10g20680 pycom10g20710 pycom10g20740 pycom13g00400 pycom15g08290
rosa_chinensis RchiOBHm_Chr1g0326861 RchiOBHm_Chr1g0326871 RchiOBHm_Chr1g0331391 RchiOBHm_Chr1g0336911 RchiOBHm_Chr1g0364001 RchiOBHm_Chr2g0174541 RchiOBHm_Chr2g0175041 RchiOBHm_Chr2g0175101 RchiOBHm_Chr2g0175121 RchiOBHm_Chr3g0486331 RchiOBHm_Chr5g0015581 RchiOBHm_Chr5g0016551 RchiOBHm_Chr5g0016561 RchiOBHm_Chr5g0016581 RchiOBHm_Chr5g0016601 RchiOBHm_Chr5g0016611 RchiOBHm_Chr5g0016641 RchiOBHm_Chr5g0016661 RchiOBHm_Chr5g0016691 RchiOBHm_Chr5g0016701 RchiOBHm_Chr5g0016731 RchiOBHm_Chr5g0016741 RchiOBHm_Chr5g0016811 RchiOBHm_Chr5g0016841 RchiOBHm_Chr5g0016871 RchiOBHm_Chr5g0016881 RchiOBHm_Chr5g0016931 RchiOBHm_Chr5g0017021 RchiOBHm_Chr5g0017041 RchiOBHm_Chr5g0017071 RchiOBHm_Chr5g0017091 RchiOBHm_Chr5g0017121 RchiOBHm_Chr5g0017141 RchiOBHm_Chr5g0017291 RchiOBHm_Chr5g0017321 RchiOBHm_Chr5g0017401 RchiOBHm_Chr5g0019001 RchiOBHm_Chr5g0019071 RchiOBHm_Chr5g0028941 RchiOBHm_Chr5g0052141 RchiOBHm_Chr5g0054721 RchiOBHm_Chr5g0054801 RchiOBHm_Chr5g0054931 RchiOBHm_Chr5g0054941 RchiOBHm_Chr6g0259291 RchiOBHm_Chr6g0292991 RchiOBHm_Chr6g0306911
rosa_laevigata RLG00000009465 RLG00000009468 RLG00000010755 RLG00000011976 RLG00000014541 RLG00000014542 RLG00000015169 RLG00000022308 RLG00000027529 RLG00000029324 RLG00000030244 RLG00000030245 RLG00000032210 RLG00000032211 RLG00000032257 RLG00000032259 RLG00000032260 RLG00000032261 RLG00000032262 RLG00000032268 RLG00000032269 RLG00000032272 RLG00000032282 RLG00000032283 RLG00000032308 RLG00000032413 RLG00000033129 RLG00000034945
rosa_multiflora Rmu_co8241929.1_g000001 Rmu_co8269227.1_g000001 Rmu_co8461259.1_g000001 Rmu_co8495385.1_g000001 Rmu_sc0000028.1_g000003 Rmu_sc0000083.1_g000009 Rmu_sc0000083.1_g000010 Rmu_sc0000083.1_g000031 Rmu_sc0000083.1_g000043 Rmu_sc0000083.1_g000046 Rmu_sc0000083.1_g000047 Rmu_sc0000563.1_g000024 Rmu_sc0000842.1_g000012 Rmu_sc0001235.1_g000001 Rmu_sc0001235.1_g000005 Rmu_sc0001235.1_g000009 Rmu_sc0001235.1_g000015 Rmu_sc0001235.1_g000016 Rmu_sc0001235.1_g000017 Rmu_sc0001473.1_g000036 Rmu_sc0001607.1_g000016 Rmu_sc0001788.1_g000006 Rmu_sc0002018.1_g000008 Rmu_sc0002028.1_g000001 Rmu_sc0002680.1_g000011 Rmu_sc0002798.1_g000023 Rmu_sc0002886.1_g000023 Rmu_sc0002989.1_g000012 Rmu_sc0003064.1_g000009 Rmu_sc0003382.1_g000006 Rmu_sc0003630.1_g000002 Rmu_sc0003630.1_g000047 Rmu_sc0003630.1_g000066 Rmu_sc0003630.1_g000067 Rmu_sc0003649.1_g000010 Rmu_sc0003688.1_g000005 Rmu_sc0003920.1_g000007 Rmu_sc0004765.1_g000007 Rmu_sc0004765.1_g000008 Rmu_sc0004920.1_g000011 Rmu_sc0005223.1_g000008 Rmu_sc0005223.1_g000010 Rmu_sc0005223.1_g000015 Rmu_sc0005223.1_g000016 Rmu_sc0005319.1_g000014 Rmu_sc0005319.1_g000015 Rmu_sc0005319.1_g000018 Rmu_sc0005319.1_g000019 Rmu_sc0005319.1_g000025 Rmu_sc0005418.1_g000007 Rmu_sc0005418.1_g000012 Rmu_sc0006106.1_g000011 Rmu_sc0006914.1_g000012 Rmu_sc0007023.1_g000001 Rmu_sc0007662.1_g000001 Rmu_sc0007920.1_g000002 Rmu_sc0007920.1_g000013 Rmu_sc0008053.1_g000016 Rmu_sc0008053.1_g000017 Rmu_sc0008053.1_g000018 Rmu_sc0008053.1_g000023 Rmu_sc0008053.1_g000050 Rmu_sc0009042.1_g000007 Rmu_sc0009042.1_g000008 Rmu_sc0010642.1_g000008 Rmu_sc0011109.1_g000002 Rmu_sc0011314.1_g000006 Rmu_sc0011974.1_g000006 Rmu_sc0012179.1_g000009 Rmu_sc0015169.1_g000001 Rmu_sc0016368.1_g000002 Rmu_sc0022659.1_g000001 Rmu_sc0027371.1_g000001 Rmu_sc0028213.1_g000001 Rmu_sc0034526.1_g000001 Rmu_sc0039366.1_g000002 Rmu_ssc0000050.1_g000084 Rmu_ssc0000050.1_g000088 Rmu_ssc0000167.1_g000017
rosa_roxburghii Rroxscaffold_1G00011340 Rroxscaffold_1G00025710 Rroxscaffold_1G00025870 Rroxscaffold_1G00026070 Rroxscaffold_1G00026170 Rroxscaffold_1G00026200 Rroxscaffold_1G00026270 Rroxscaffold_1G00050950 Rroxscaffold_1G00058900 Rroxscaffold_1G00060040 Rroxscaffold_1G00060060 Rroxscaffold_1G00060070 Rroxscaffold_1G00060080 Rroxscaffold_1G00060110 Rroxscaffold_1G00060120 Rroxscaffold_1G00060140 Rroxscaffold_1G00060170 Rroxscaffold_1G00060210 Rroxscaffold_1G00060220 Rroxscaffold_1G00060240 Rroxscaffold_1G00060250 Rroxscaffold_1G00060260 Rroxscaffold_1G00060300 Rroxscaffold_1G00060320 Rroxscaffold_1G00060330 Rroxscaffold_1G00060900 Rroxscaffold_1G00060920 Rroxscaffold_1G00073980 Rroxscaffold_2G00077560 Rroxscaffold_2G00077580 Rroxscaffold_2G00146990 Rroxscaffold_3G00236200 Rroxscaffold_3G00236210 Rroxscaffold_3G00236220 Rroxscaffold_4G00292760 Rroxscaffold_4G00315200 Rroxscaffold_4G00315240 Rroxscaffold_4G00326240 Rroxscaffold_4G00328980 Rroxscaffold_5G00341890 Rroxscaffold_7G00161350 Rroxscaffold_7G00174490 Rroxscaffold_7G00206550
rosa_rugosa Rorug01G0093000 Rorug01G0093100 Rorug01G0128800 Rorug01G0312000 Rorug01G0312000 Rorug02G0581100 Rorug03G0224600 Rorug05G0028500 Rorug05G0032700 Rorug05G0032800 Rorug05G0032900 Rorug05G0032900 Rorug05G0033000 Rorug05G0033100 Rorug05G0033300 Rorug05G0033400 Rorug05G0033900 Rorug05G0034900 Rorug05G0035000 Rorug05G0046900 Rorug05G0047500 Rorug05G0047500 Rorug05G0111600 Rorug05G0287900 Rorug05G0288700 Rorug05G0586900 Rorug06G0231700 Rorug06G0354300 Rorug06G0354400
rosa_samantha Rh1DG122200 Rh2DG686800 Rh5BG136600 Rh6AG344500 Rh6BG351900 Rh6CG359000 Rh6CG359100 Rh6CG387600 Rh6CG480500 Rh6DG344600 Rh6DG467400
rosa_wichuraiana Rw0G010250 Rw0G014820 Rw0G021400 Rw0G023100 Rw1G009450 Rw1G012100 Rw1G012180 Rw1G028360 Rw2G054260 Rw3G024370 Rw3G024420 Rw3G024470 Rw5G010850 Rw5G011010 Rw5G011020 Rw5G011050 Rw5G011080 Rw5G011120 Rw5G012300 Rw5G018470 Rw5G033830 Rw6G008930 Rw6G030070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 174
Acc65I GGTACC 1 cut(s) 358
AccB1I GGYRCC 1 cut(s) 358
AccB7I CCANNNNNTGG 1 cut(s) 2249
AccI GTMKAC 3 cut(s) 261, 1309, 1473
AccII CGCG 1 cut(s) 1571
AciI CCGC 6 cut(s) 32, 56, 117, 295, 756, 1571
AcoI YGGCCR 1 cut(s) 1971
AcsI RAATTY 4 cut(s) 176, 452, 1083, 1478
AcuI CTGAAG 2 cut(s) 1248, 1689
AfaI GTAC 7 cut(s) 64, 360, 368, 639, 845, 1142, 2200
AfeI AGCGCT 1 cut(s) 1578
AfiI CCNNNNNNNGG 6 cut(s) 31, 52, 264, 718, 926, 2249
AgeI ACCGGT 1 cut(s) 780
AgsI TTSAA 6 cut(s) 556, 677, 685, 1147, 1541, 1912
AhlI ACTAGT 1 cut(s) 1468
AjnI CCWGG 4 cut(s) 97, 823, 925, 1752
AjuI GAANNNNNNNTTGG 4 cut(s) 377, 409, 2106, 2138
AluBI AGCT 9 cut(s) 349, 582, 624, 761, 1197, 1432, 1688, 1809, 1831
AluI AGCT 9 cut(s) 349, 582, 624, 761, 1197, 1432, 1688, 1809, 1831
Alw21I GWGCWC 1 cut(s) 351
Alw26I GTCTC 3 cut(s) 1005, 1949, 2102
AlwNI CAGNNNCTG 1 cut(s) 344
Aor51HI AGCGCT 1 cut(s) 1578
AoxI GGCC 3 cut(s) 12, 1971, 2015
ApeKI GCWGC 4 cut(s) 83, 102, 621, 1355
ApoI RAATTY 4 cut(s) 176, 452, 1083, 1478
AsiGI ACCGGT 1 cut(s) 780
Asp700I GAANNNNTTC 2 cut(s) 392, 699
Asp718I GGTACC 1 cut(s) 358
AspLEI GCGC 3 cut(s) 1462, 1571, 1579
AspS9I GGNCC 2 cut(s) 709, 1756
AsuHPI GGTGA 3 cut(s) 1402, 1948, 2180
AvaII GGWCC 2 cut(s) 709, 1756
BaeI ACNNNNGTAYC 2 cut(s) 350, 383
BalI TGGCCA 1 cut(s) 1973
BanI GGYRCC 1 cut(s) 358
BanII GRGCYC 1 cut(s) 351
BarI GAAGNNNNNNTAC 2 cut(s) 1449, 1481
BbsI GAAGAC 1 cut(s) 1211
Bbv12I GWGCWC 1 cut(s) 351
BbvI GCAGC 4 cut(s) 70, 89, 633, 1342
BccI CCATC 2 cut(s) 1922, 2243
BceAI ACGGC 4 cut(s) 713, 985, 1680, 2233
BcgI CGANNNNNNTGC 2 cut(s) 2209, 2243
BciT130I CCWGG 4 cut(s) 99, 825, 927, 1754
BciVI GTATCC 2 cut(s) 950, 1742
BclI TGATCA 1 cut(s) 1372
BcoDI GTCTC 3 cut(s) 1005, 1949, 2102
BcuI ACTAGT 1 cut(s) 1468
BfaI CTAG 7 cut(s) 60, 546, 1185, 1469, 1823, 1979, 2181
BfmI CTRYAG 2 cut(s) 619, 798
BfoI RGCGCY 2 cut(s) 1463, 1580
BfuAI ACCTGC 1 cut(s) 174
BfuI GTATCC 2 cut(s) 950, 1742
BisI GCNGC 7 cut(s) 33, 57, 84, 103, 118, 622, 1356
BlsI GCNGC 7 cut(s) 34, 58, 85, 104, 119, 623, 1357
BmcAI AGTACT 2 cut(s) 64, 1142
Bme1390I CCNGG 4 cut(s) 99, 825, 927, 1754
Bme18I GGWCC 2 cut(s) 709, 1756
BmgT120I GGNCC 2 cut(s) 709, 1756
BmiI GGNNCC 3 cut(s) 360, 1701, 1758
BmrFI CCNGG 4 cut(s) 99, 825, 927, 1754
BmrI ACTGGG 1 cut(s) 471
BmsI GCATC 4 cut(s) 999, 1856, 1887, 1979
BmuI ACTGGG 1 cut(s) 471
BoxI GACNNNNGTC 1 cut(s) 744
BpiI GAAGAC 1 cut(s) 1211
BpmI CTGGAG 1 cut(s) 909
Bpu10I CCTNAGC 1 cut(s) 345
BpuEI CTTGAG 3 cut(s) 72, 1256, 2014
BsaBI GATNNNNATC 1 cut(s) 989
BsaJI CCNNGG 5 cut(s) 439, 1078, 1273, 1893, 2075
BsaWI WCCGGW 2 cut(s) 780, 1315
BsaXI ACNNNNNCTCC 4 cut(s) 725, 755, 1577, 1607
Bsc4I CCNNNNNNNGG 6 cut(s) 31, 52, 264, 718, 926, 2249
Bse118I RCCGGY 2 cut(s) 780, 1849
Bse1I ACTGG 5 cut(s) 466, 492, 649, 669, 2193
Bse8I GATNNNNATC 1 cut(s) 989
BseBI CCWGG 4 cut(s) 99, 825, 927, 1754
BseDI CCNNGG 5 cut(s) 439, 1078, 1273, 1893, 2075
BseGI GGATG 4 cut(s) 540, 604, 1324, 2071
BseJI GATNNNNATC 1 cut(s) 989
BseLI CCNNNNNNNGG 6 cut(s) 31, 52, 264, 718, 926, 2249
BseMII CTCAG 1 cut(s) 336
BseNI ACTGG 5 cut(s) 466, 492, 649, 669, 2193
BseRI GAGGAG 1 cut(s) 1585
BseXI GCAGC 4 cut(s) 70, 89, 633, 1342
BsgI GTGCAG 1 cut(s) 152
Bsh1236I CGCG 1 cut(s) 1571
BshFI GGCC 3 cut(s) 14, 1973, 2017
BshNI GGYRCC 1 cut(s) 358
BshTI ACCGGT 1 cut(s) 780
BsiHKAI GWGCWC 1 cut(s) 351
BsiSI CCGG 3 cut(s) 781, 1316, 1850
BslFI GGGAC 4 cut(s) 620, 734, 1567, 1753
BslI CCNNNNNNNGG 6 cut(s) 31, 52, 264, 718, 926, 2249
BsmAI GTCTC 3 cut(s) 1005, 1949, 2102
BsmBI CGTCTC 1 cut(s) 1949
BsmFI GGGAC 4 cut(s) 620, 734, 1567, 1753
BsnI GGCC 3 cut(s) 14, 1973, 2017
Bsp1286I GDGCHC 1 cut(s) 351
Bsp1407I TGTACA 1 cut(s) 637
Bsp143I GATC 4 cut(s) 6, 1020, 1372, 1523
Bsp19I CCATGG 1 cut(s) 1893
BspACI CCGC 6 cut(s) 32, 56, 117, 295, 756, 1571
BspANI GGCC 3 cut(s) 14, 1973, 2017
BspCNI CTCAG 1 cut(s) 337
BspFNI CGCG 1 cut(s) 1571
BspHI TCATGA 1 cut(s) 1258
BspLI GGNNCC 3 cut(s) 360, 1701, 1758
BspMAI CTGCAG 1 cut(s) 623
BspMI ACCTGC 1 cut(s) 174
BspT107I GGYRCC 1 cut(s) 358
BsrFI RCCGGY 2 cut(s) 780, 1849
BsrGI TGTACA 1 cut(s) 637
BsrI ACTGG 5 cut(s) 466, 492, 649, 669, 2193
BssAI RCCGGY 2 cut(s) 780, 1849
BssECI CCNNGG 5 cut(s) 439, 1078, 1273, 1893, 2075
BssMI GATC 4 cut(s) 6, 1020, 1372, 1523
BssNAI GTATAC 2 cut(s) 1310, 1474
BssT1I CCWWGG 3 cut(s) 1078, 1273, 1893
Bst1107I GTATAC 2 cut(s) 1310, 1474
Bst2UI CCWGG 4 cut(s) 99, 825, 927, 1754
Bst4CI ACNGT 5 cut(s) 130, 906, 1297, 1960, 2235
Bst6I CTCTTC 1 cut(s) 2100
BstAUI TGTACA 1 cut(s) 637
BstC8I GCNNGC 2 cut(s) 754, 2222
BstDEI CTNAG 1 cut(s) 345
BstDSI CCRYGG 2 cut(s) 1893, 2075
BstF5I GGATG 4 cut(s) 540, 604, 1324, 2071
BstFNI CGCG 1 cut(s) 1571
BstH2I RGCGCY 2 cut(s) 1463, 1580
BstHHI GCGC 3 cut(s) 1462, 1571, 1579
BstKTI GATC 4 cut(s) 9, 1023, 1375, 1526
BstMAI GTCTC 3 cut(s) 1005, 1949, 2102
BstMBI GATC 4 cut(s) 6, 1020, 1372, 1523
BstMWI GCNNNNNNNGC 5 cut(s) 65, 89, 758, 1875, 1979
BstNI CCWGG 4 cut(s) 99, 825, 927, 1754
BstNSI RCATGY 4 cut(s) 472, 756, 772, 2224
BstPAI GACNNNNGTC 1 cut(s) 744
BstSCI CCNGG 4 cut(s) 97, 823, 925, 1752
BstSFI CTRYAG 2 cut(s) 619, 798
BstUI CGCG 1 cut(s) 1571
BstV1I GCAGC 4 cut(s) 70, 89, 633, 1342
BstV2I GAAGAC 1 cut(s) 1211
BstZ17I GTATAC 2 cut(s) 1310, 1474
BsuI GTATCC 2 cut(s) 950, 1742
BsuRI GGCC 3 cut(s) 14, 1973, 2017
BtgI CCRYGG 2 cut(s) 1893, 2075
BtsCI GGATG 4 cut(s) 540, 604, 1324, 2071
BtsI GCAGTG 1 cut(s) 2205
BtsIMutI CAGTG 5 cut(s) 499, 690, 911, 2200, 2205
BveI ACCTGC 1 cut(s) 174
Cac8I GCNNGC 2 cut(s) 754, 2222
CaiI CAGNNNCTG 1 cut(s) 344
CciI TCATGA 1 cut(s) 1258
CfoI GCGC 3 cut(s) 1462, 1571, 1579
Cfr10I RCCGGY 2 cut(s) 780, 1849
Cfr13I GGNCC 2 cut(s) 709, 1756
CseI GACGC 1 cut(s) 2150
Csp6I GTAC 7 cut(s) 63, 359, 367, 638, 844, 1141, 2199
CspAI ACCGGT 1 cut(s) 780
CviQI GTAC 7 cut(s) 63, 359, 367, 638, 844, 1141, 2199
DdeI CTNAG 1 cut(s) 345
DpnI GATC 4 cut(s) 8, 1022, 1374, 1525
DpnII GATC 4 cut(s) 6, 1020, 1372, 1523
EaeI YGGCCR 1 cut(s) 1971
Eam1104I CTCTTC 1 cut(s) 2100
EarI CTCTTC 1 cut(s) 2100
Ecl136II GAGCTC 1 cut(s) 349
Eco130I CCWWGG 3 cut(s) 1078, 1273, 1893
Eco147I AGGCCT 1 cut(s) 2017
Eco24I GRGCYC 1 cut(s) 351
Eco47I GGWCC 2 cut(s) 709, 1756
Eco47III AGCGCT 1 cut(s) 1578
Eco53kI GAGCTC 1 cut(s) 349
Eco57I CTGAAG 2 cut(s) 1248, 1689
EcoICRI GAGCTC 1 cut(s) 349
EcoRI GAATTC 3 cut(s) 176, 452, 1478
EcoRII CCWGG 4 cut(s) 97, 823, 925, 1752
EcoT14I CCWWGG 3 cut(s) 1078, 1273, 1893
EcoT38I GRGCYC 1 cut(s) 351
ErhI CCWWGG 3 cut(s) 1078, 1273, 1893
Esp3I CGTCTC 1 cut(s) 1949
FaqI GGGAC 4 cut(s) 620, 734, 1567, 1753
FauI CCCGC 1 cut(s) 288
FbaI TGATCA 1 cut(s) 1372
FblI GTMKAC 3 cut(s) 261, 1309, 1473
Fnu4HI GCNGC 7 cut(s) 33, 57, 84, 103, 118, 622, 1356
FokI GGATG 4 cut(s) 527, 611, 1331, 2058
FriOI GRGCYC 1 cut(s) 351
Fsp4HI GCNGC 7 cut(s) 33, 57, 84, 103, 118, 622, 1356
FspBI CTAG 7 cut(s) 60, 546, 1185, 1469, 1823, 1979, 2181
GlaI GCGC 3 cut(s) 1461, 1570, 1578
GluI GCNGC 7 cut(s) 33, 57, 84, 103, 118, 622, 1356
GsuI CTGGAG 1 cut(s) 909
HaeII RGCGCY 2 cut(s) 1463, 1580
HaeIII GGCC 3 cut(s) 14, 1973, 2017
HapII CCGG 3 cut(s) 781, 1316, 1850
HgaI GACGC 1 cut(s) 2150
HhaI GCGC 3 cut(s) 1462, 1571, 1579
Hin6I GCGC 3 cut(s) 1460, 1569, 1577
HinP1I GCGC 3 cut(s) 1460, 1569, 1577
HincII GTYRAC 1 cut(s) 1384
HindII GTYRAC 1 cut(s) 1384
HinfI GANTC 6 cut(s) 235, 740, 1001, 1013, 1269, 1912
HpaI GTTAAC 1 cut(s) 1384
HpaII CCGG 3 cut(s) 781, 1316, 1850
HphI GGTGA 3 cut(s) 1402, 1948, 2180
Hpy166II GTNNAC 8 cut(s) 262, 369, 1300, 1310, 1384, 1474, 1804, 2255
Hpy188I TCNGA 5 cut(s) 109, 379, 440, 1678, 2140
Hpy188III TCNNGA 3 cut(s) 1259, 1622, 1692
Hpy8I GTNNAC 8 cut(s) 262, 369, 1300, 1310, 1384, 1474, 1804, 2255
Hpy99I CGWCG 1 cut(s) 971
HpyAV CCTTC 9 cut(s) 640, 827, 848, 947, 1234, 1274, 1713, 1895, 2159
HpyCH4III ACNGT 5 cut(s) 130, 906, 1297, 1960, 2235
HpyF10VI GCNNNNNNNGC 5 cut(s) 65, 89, 758, 1875, 1979
HpyF3I CTNAG 1 cut(s) 345
HspAI GCGC 3 cut(s) 1460, 1569, 1577
KpnI GGTACC 1 cut(s) 362
Ksp22I TGATCA 1 cut(s) 1372
KspAI GTTAAC 1 cut(s) 1384
Kzo9I GATC 4 cut(s) 6, 1020, 1372, 1523
LmnI GCTCC 3 cut(s) 758, 1685, 1828
Lsp1109I GCAGC 4 cut(s) 70, 89, 633, 1342
LweI GCATC 4 cut(s) 999, 1856, 1887, 1979
MaeI CTAG 7 cut(s) 60, 546, 1185, 1469, 1823, 1979, 2181
MaeIII GTNAC 6 cut(s) 161, 244, 302, 410, 947, 1167
MalI GATC 4 cut(s) 8, 1022, 1374, 1525
MboI GATC 4 cut(s) 6, 1020, 1372, 1523
MboII GAAGA 7 cut(s) 458, 1120, 1216, 1241, 1564, 1910, 2117
MfeI CAATTG 1 cut(s) 187
MhlI GDGCHC 1 cut(s) 351
MlsI TGGCCA 1 cut(s) 1973
MluNI TGGCCA 1 cut(s) 1973
MlyI GAGTC 3 cut(s) 734, 1007, 1278
Mox20I TGGCCA 1 cut(s) 1973
MroXI GAANNNNTTC 2 cut(s) 392, 699
MscI TGGCCA 1 cut(s) 1973
MseI TTAA 9 cut(s) 612, 714, 911, 1125, 1383, 1871, 2085, 2133, 2237
MslI CAYNNNNRTG 5 cut(s) 597, 1263, 1419, 1623, 2250
Msp20I TGGCCA 1 cut(s) 1973
MspI CCGG 3 cut(s) 781, 1316, 1850
MspR9I CCNGG 4 cut(s) 99, 825, 927, 1754
MunI CAATTG 1 cut(s) 187
MvaI CCWGG 4 cut(s) 99, 825, 927, 1754
MvnI CGCG 1 cut(s) 1571
MwoI GCNNNNNNNGC 5 cut(s) 65, 89, 758, 1875, 1979
NcoI CCATGG 1 cut(s) 1893
NdeII GATC 4 cut(s) 6, 1020, 1372, 1523
NlaIV GGNNCC 3 cut(s) 360, 1701, 1758
NmuCI GTSAC 1 cut(s) 410
NspI RCATGY 4 cut(s) 472, 756, 772, 2224
PaeI GCATGC 2 cut(s) 756, 2224
PagI TCATGA 1 cut(s) 1258
PceI AGGCCT 1 cut(s) 2017
PcsI WCGNNNNNNNCGW 1 cut(s) 2207
PdmI GAANNNNTTC 2 cut(s) 392, 699
PfeI GAWTC 3 cut(s) 235, 1001, 1912
PflMI CCANNNNNTGG 1 cut(s) 2249
PfoI TCCNGGA 1 cut(s) 925
PinAI ACCGGT 1 cut(s) 780
PkrI GCNGC 7 cut(s) 34, 58, 85, 104, 119, 623, 1357
PleI GAGTC 3 cut(s) 734, 1007, 1277
PpsI GAGTC 3 cut(s) 734, 1007, 1277
PshAI GACNNNNGTC 1 cut(s) 744
Psp124BI GAGCTC 1 cut(s) 351
Psp6I CCWGG 4 cut(s) 97, 823, 925, 1752
PspGI CCWGG 4 cut(s) 97, 823, 925, 1752
PspN4I GGNNCC 3 cut(s) 360, 1701, 1758
PspPI GGNCC 2 cut(s) 709, 1756
PsrI GAACNNNNNNTAC 2 cut(s) 380, 412
PstI CTGCAG 1 cut(s) 623
PstNI CAGNNNCTG 1 cut(s) 344
RsaI GTAC 7 cut(s) 64, 360, 368, 639, 845, 1142, 2200
RsaNI GTAC 7 cut(s) 63, 359, 367, 638, 844, 1141, 2199
RseI CAYNNNNRTG 5 cut(s) 597, 1263, 1419, 1623, 2250
SacI GAGCTC 1 cut(s) 351
SaqAI TTAA 9 cut(s) 612, 714, 911, 1125, 1383, 1871, 2085, 2133, 2237
SatI GCNGC 7 cut(s) 33, 57, 84, 103, 118, 622, 1356
Sau3AI GATC 4 cut(s) 6, 1020, 1372, 1523
Sau96I GGNCC 2 cut(s) 709, 1756
ScaI AGTACT 2 cut(s) 64, 1142
SchI GAGTC 3 cut(s) 734, 1007, 1278
ScrFI CCNGG 4 cut(s) 99, 825, 927, 1754
SduI GDGCHC 1 cut(s) 351
SfaNI GCATC 4 cut(s) 999, 1856, 1887, 1979
SfcI CTRYAG 2 cut(s) 619, 798
SinI GGWCC 2 cut(s) 709, 1756
SmiMI CAYNNNNRTG 5 cut(s) 597, 1263, 1419, 1623, 2250
SmlI CTYRAG 3 cut(s) 87, 1235, 1993
SmoI CTYRAG 3 cut(s) 87, 1235, 1993
SpeI ACTAGT 1 cut(s) 1468
SphI GCATGC 2 cut(s) 756, 2224
SseBI AGGCCT 1 cut(s) 2017
SsiI CCGC 6 cut(s) 32, 56, 117, 295, 756, 1571
SspI AATATT 2 cut(s) 916, 1642
SspMI CTAG 7 cut(s) 60, 546, 1185, 1469, 1823, 1979, 2181
SstI GAGCTC 1 cut(s) 351
StuI AGGCCT 1 cut(s) 2017
StyD4I CCNGG 4 cut(s) 97, 823, 925, 1752
StyI CCWWGG 3 cut(s) 1078, 1273, 1893
TaaI ACNGT 5 cut(s) 130, 906, 1297, 1960, 2235
TaqI TCGA 6 cut(s) 5, 322, 1199, 1346, 1621, 2229
TatI WGTACW 3 cut(s) 62, 637, 1140
TauI GCSGC 3 cut(s) 35, 59, 120
TfiI GAWTC 3 cut(s) 235, 1001, 1912
Tru1I TTAA 9 cut(s) 612, 714, 911, 1125, 1383, 1871, 2085, 2133, 2237
Tru9I TTAA 9 cut(s) 612, 714, 911, 1125, 1383, 1871, 2085, 2133, 2237
TscAI CASTG 5 cut(s) 499, 697, 911, 2200, 2212
TseFI GTSAC 1 cut(s) 410
TseI GCWGC 4 cut(s) 83, 102, 621, 1355
Tsp45I GTSAC 1 cut(s) 410
TspGWI ACGGA 4 cut(s) 247, 354, 915, 2064
TspRI CASTG 5 cut(s) 499, 697, 911, 2200, 2212
Van91I CCANNNNNTGG 1 cut(s) 2249
VpaK11BI GGWCC 2 cut(s) 709, 1756
XapI RAATTY 4 cut(s) 176, 452, 1083, 1478
XceI RCATGY 4 cut(s) 472, 756, 772, 2224
XmiI GTMKAC 3 cut(s) 261, 1309, 1473
XmnI GAANNNNTTC 2 cut(s) 392, 699
XspI CTAG 7 cut(s) 60, 546, 1185, 1469, 1823, 1979, 2181
ZrmI AGTACT 2 cut(s) 64, 1142
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.