RchiOBHm_Chr5g0052141

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000066
Physical Location & Seq
Reverse (-)
53754840 .. 53757639
2800 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ32956

Sequence Viewer

Length: 2256 bp
ATGGCGTTGCGACTCTCATTGGTGGCAGTCCTACTGCTGTTATCGGCGGCTAGTACTACTACGACAACAGCAGCTCCTGCTCTAGCCAAGTCTGGCTGCCCAGACAAGTGCGGCAATCTCACAATTCCATACCCATTTGGCATGGAGGAGGGTTGTTACCTGCGAAAAGAATTCTCTATCAATTGTAGCAATACCACCCAACCACCAACAGCATATTTTGCGAAAGACAGCAATATCGTTGTTACTAACATTTCCCTTGATGAAGGTGAGGTGCGAATATTAAACTTCATAGCTTCAGATTGCTATGAAGCCCCCGGCAATACGACATCCGGGTTTTTCTCTGAGATCACCCTGAATCTTCCTTATACGATATCTGATACCAAAAACAGATTCATTGTCGTTGGTTGTGACACTTACGCATTTCTTCGAGGCTACCGAGTGGAAGAAGGTGATCAGTTCTCAACTGGATGCATGTCCATATGCAACAGCCTCGACAGCGTTGATAAGAACTCTTGCTCCGGTGTTGGGTGTTGCCAGACTAACACCCCCAGCGGGCTGTACAATACGAGGGTGACTGTGAATAGCTTCTACAACTATTCGGATGTATGGAAGTTTAACCCCTGCAGCTACGCCTTCATTGCGGAACAAGGCCAGTTCAATTTCTCCCCTGCGAGTTTTGAACAACTGAATGGCACTGACCAGGTTCCAATAGTTCTTAATTGGGCTATTGGGAATGACAATGACCCTTGTGATGAAGCTCAAAAGAGGGAGGATTTCGCTTGCAAGGCTCATAGCAAGTGCGTCAACAGGCCTATCAATGGGTCTATAGGTTACTTGTGCCAGTGCTTGCCTGGTTACGAAGGAAATCCCTACCATCCAGATGGTTGCCAAGATATTGATGAGTGCGAGGTTCCAAACCTCTGCCAGCATGGAAAGTGCTTAAATTCACAAGGAAATTACTCTTGTTCATGTCACAAAGGATACAAACATGACGGCATGAATGACAAGATCTGCATCAAAAAAGTCTCGAAGATGGAAGTCCTGCTTATAGTTTCATTGGGTGTAAGTGTAGGCTTCTTGGTTTCATTCATTGGAATTGCATGGTTATGGTGGGCAAATAAGAAAAGACAATTCATCAAACTGAAAAAAAAGTACTTCAAAGAAAATGGAGGCTTATTGTTACAACAACAACTTGCCAGTCATGGAGGTACTATAGACACAACAAAAATTTTTAGTGCAGAAGAACTTCAGAAGGCTACAAACAATTACCATGAAAGTAGGGTTCTTGGCAAAGGAGGCTATGGAATAGTATACAAAGGTATACTACCAGATAACCAAGTGATTGCCATAAAGAAGTCAAAAGTTTGTGCCGGCAGTCAGAGTGAGCAATTTGTCAATGAGGTAATCATTCTTTCTCAAATCAACCACAGAAATGTTGTGAAGCTTTTGGGTTGTTGTTTAGAAACAGAAGTGCCTATACTTGTATACGAGTTCATCAGCCATGGCACTCTTCATGAGCACATTCATAAGAAAAGATCATCACTCGCATTTGAATTACGAATGAAGATAGCAGCTGAAGCAGCAGGAGCACTAGCCCATTTACACTCCTCAATTTCCACGCCAATCATACATCGAGATGTCAAAGCAGCGAATATTCTGTTAGATAATAAGTATACGGCAAAAGTGTCAGACTTTGGAGCTTCACGATTGGTTTCTTCAGGTGAAGCTGGAATACAAACTGTAGTGCTCGGGACAATAGGATACCTGGACCCTGAATATCTGCATTCAAACCAACTAACAGAAAAGAGTGACGTTTACAGCTTTGGGGTTCTCTTGGCTGAGCTTTTAACAAGCAAAGTGGCAGTTTCTAAAGATAAATTCTTAGCAAGCATCTTTGTTGCTTCCATGGAAACAGATTGCTTAAATCAAGTGCTTGATGATGAGATAGTGAATGAAGGAAATATTGAGATGGTGAAATATGTGGCCAAACTTGCAAGAAGATGTTTGAGGGTAAAAGGGGAGGAAAGGCCTACCATGAGAGAAGTAGCCATGGAGCTAGGAGGAATGATCATGACAAAGCATCCATGGGGTTCTGCAGATCATTACTTTCCAGAAGAGACAGAGCACTTGCTCGGATCACCTAATACTTCCAAGGATTACTTTGTGAATATTGATGGTGGTGGTGGTCCTGGTACTGCCACTGGGTCTAGCATGCAAATAGACATGTCAATGTCTTCATATGCTGATGGGCGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK

Protein Analysis

751

Amino Acids

82.38

Weight (kDa)

5.98

Isoelectric Point (pI)

33.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 33 - 94 5.8e-12 Wall-associated receptor kinase galacturonan-binding
EGF_CA PF07645 298 - 334 8e-07 Calcium-binding EGF domain
Pkinase PF00069 423 - 682 2.1e-48 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 426 - 686 2.1e-48 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21210 AT1G21230 AT1G21240 AT1G21240 AT1G21245 AT1G21250 AT1G21270 AT1G22720 AT3G25490
fragaria_vesca FvH4_2g35051 FvH4_2g35051 FvH4_2g35060 FvH4_3g10281 FvH4_3g10285 FvH4_3g10286 FvH4_3g10311 FvH4_3g10311 FvH4_3g10320 FvH4_3g10332 FvH4_3g10340 FvH4_3g10350 FvH4_3g10360 FvH4_3g10390 FvH4_3g10412 FvH4_3g10413 FvH4_3g10461 FvH4_3g10462 FvH4_3g10470 FvH4_3g10510 FvH4_3g10520 FvH4_3g10530 FvH4_3g10550 FvH4_3g10560 FvH4_3g10611 FvH4_3g11730 FvH4_6g53140 FvH4_6g53142 FvH4_7g31880 FvH4_7g31910 FvH4_7g31920
malus_domestica MD05G1269900.v1.1 MD05G1270000.v1.1 MD05G1270200.v1.1 MD05G1270400.v1.1 MD05G1270500.v1.1 MD05G1270600.v1.1 MD05G1270800.v1.1 MD05G1270900.v1.1 MD07G1183800.v1.1 MD08G1107600.v1.1 MD10G1248500.v1.1 MD10G1249200.v1.1 MD10G1249500.v1.1 MD10G1250000.v1.1 MD10G1250500.v1.1 MD10G1250900.v1.1 MD10G1251200.v1.1 MD10G1251400.v1.1 MD13G1004600.v1.1 MD15G1088400.v1.1
prunus_persica Prupe.1G442400_v2.0.a1 Prupe.1G442500_v2.0.a1 Prupe.2G223400_v2.0.a1 Prupe.4G091900_v2.0.a1 Prupe.4G093200_v2.0.a1 Prupe.4G093300_v2.0.a1 Prupe.4G093400_v2.0.a1 Prupe.4G093500_v2.0.a1 Prupe.4G094100_v2.0.a1 Prupe.4G094200_v2.0.a1 Prupe.4G094300_v2.0.a1 Prupe.6G358300_v2.0.a1
pyrus_communis pycom08g08990 pycom10g20660 pycom10g20670 pycom10g20680 pycom10g20710 pycom10g20740 pycom13g00400 pycom15g08290
rosa_chinensis RchiOBHm_Chr1g0326861 RchiOBHm_Chr1g0326871 RchiOBHm_Chr1g0331391 RchiOBHm_Chr1g0336911 RchiOBHm_Chr1g0364001 RchiOBHm_Chr2g0174541 RchiOBHm_Chr2g0175041 RchiOBHm_Chr2g0175101 RchiOBHm_Chr2g0175121 RchiOBHm_Chr3g0486331 RchiOBHm_Chr5g0015581 RchiOBHm_Chr5g0016551 RchiOBHm_Chr5g0016561 RchiOBHm_Chr5g0016581 RchiOBHm_Chr5g0016601 RchiOBHm_Chr5g0016611 RchiOBHm_Chr5g0016641 RchiOBHm_Chr5g0016661 RchiOBHm_Chr5g0016691 RchiOBHm_Chr5g0016701 RchiOBHm_Chr5g0016731 RchiOBHm_Chr5g0016741 RchiOBHm_Chr5g0016811 RchiOBHm_Chr5g0016841 RchiOBHm_Chr5g0016871 RchiOBHm_Chr5g0016881 RchiOBHm_Chr5g0016931 RchiOBHm_Chr5g0017021 RchiOBHm_Chr5g0017041 RchiOBHm_Chr5g0017071 RchiOBHm_Chr5g0017091 RchiOBHm_Chr5g0017121 RchiOBHm_Chr5g0017141 RchiOBHm_Chr5g0017291 RchiOBHm_Chr5g0017321 RchiOBHm_Chr5g0017401 RchiOBHm_Chr5g0019001 RchiOBHm_Chr5g0019071 RchiOBHm_Chr5g0028941 RchiOBHm_Chr5g0052141 RchiOBHm_Chr5g0054721 RchiOBHm_Chr5g0054801 RchiOBHm_Chr5g0054931 RchiOBHm_Chr5g0054941 RchiOBHm_Chr6g0259291 RchiOBHm_Chr6g0292991 RchiOBHm_Chr6g0306911
rosa_laevigata RLG00000009465 RLG00000009468 RLG00000010755 RLG00000011976 RLG00000014541 RLG00000014542 RLG00000015169 RLG00000022308 RLG00000027529 RLG00000029324 RLG00000030244 RLG00000030245 RLG00000032210 RLG00000032211 RLG00000032257 RLG00000032259 RLG00000032260 RLG00000032261 RLG00000032262 RLG00000032268 RLG00000032269 RLG00000032272 RLG00000032282 RLG00000032283 RLG00000032308 RLG00000032413 RLG00000033129 RLG00000034945
rosa_multiflora Rmu_co8241929.1_g000001 Rmu_co8269227.1_g000001 Rmu_co8461259.1_g000001 Rmu_co8495385.1_g000001 Rmu_sc0000028.1_g000003 Rmu_sc0000083.1_g000009 Rmu_sc0000083.1_g000010 Rmu_sc0000083.1_g000031 Rmu_sc0000083.1_g000043 Rmu_sc0000083.1_g000046 Rmu_sc0000083.1_g000047 Rmu_sc0000563.1_g000024 Rmu_sc0000842.1_g000012 Rmu_sc0001235.1_g000001 Rmu_sc0001235.1_g000005 Rmu_sc0001235.1_g000009 Rmu_sc0001235.1_g000015 Rmu_sc0001235.1_g000016 Rmu_sc0001235.1_g000017 Rmu_sc0001473.1_g000036 Rmu_sc0001607.1_g000016 Rmu_sc0001788.1_g000006 Rmu_sc0002018.1_g000008 Rmu_sc0002028.1_g000001 Rmu_sc0002680.1_g000011 Rmu_sc0002798.1_g000023 Rmu_sc0002886.1_g000023 Rmu_sc0002989.1_g000012 Rmu_sc0003064.1_g000009 Rmu_sc0003382.1_g000006 Rmu_sc0003630.1_g000002 Rmu_sc0003630.1_g000047 Rmu_sc0003630.1_g000066 Rmu_sc0003630.1_g000067 Rmu_sc0003649.1_g000010 Rmu_sc0003688.1_g000005 Rmu_sc0003920.1_g000007 Rmu_sc0004765.1_g000007 Rmu_sc0004765.1_g000008 Rmu_sc0004920.1_g000011 Rmu_sc0005223.1_g000008 Rmu_sc0005223.1_g000010 Rmu_sc0005223.1_g000015 Rmu_sc0005223.1_g000016 Rmu_sc0005319.1_g000014 Rmu_sc0005319.1_g000015 Rmu_sc0005319.1_g000018 Rmu_sc0005319.1_g000019 Rmu_sc0005319.1_g000025 Rmu_sc0005418.1_g000007 Rmu_sc0005418.1_g000012 Rmu_sc0006106.1_g000011 Rmu_sc0006914.1_g000012 Rmu_sc0007023.1_g000001 Rmu_sc0007662.1_g000001 Rmu_sc0007920.1_g000002 Rmu_sc0007920.1_g000013 Rmu_sc0008053.1_g000016 Rmu_sc0008053.1_g000017 Rmu_sc0008053.1_g000018 Rmu_sc0008053.1_g000023 Rmu_sc0008053.1_g000050 Rmu_sc0009042.1_g000007 Rmu_sc0009042.1_g000008 Rmu_sc0010642.1_g000008 Rmu_sc0011109.1_g000002 Rmu_sc0011314.1_g000006 Rmu_sc0011974.1_g000006 Rmu_sc0012179.1_g000009 Rmu_sc0015169.1_g000001 Rmu_sc0016368.1_g000002 Rmu_sc0022659.1_g000001 Rmu_sc0027371.1_g000001 Rmu_sc0028213.1_g000001 Rmu_sc0034526.1_g000001 Rmu_sc0039366.1_g000002 Rmu_ssc0000050.1_g000084 Rmu_ssc0000050.1_g000088 Rmu_ssc0000167.1_g000017
rosa_roxburghii Rroxscaffold_1G00011340 Rroxscaffold_1G00025710 Rroxscaffold_1G00025870 Rroxscaffold_1G00026070 Rroxscaffold_1G00026170 Rroxscaffold_1G00026200 Rroxscaffold_1G00026270 Rroxscaffold_1G00050950 Rroxscaffold_1G00058900 Rroxscaffold_1G00060040 Rroxscaffold_1G00060060 Rroxscaffold_1G00060070 Rroxscaffold_1G00060080 Rroxscaffold_1G00060110 Rroxscaffold_1G00060120 Rroxscaffold_1G00060140 Rroxscaffold_1G00060170 Rroxscaffold_1G00060210 Rroxscaffold_1G00060220 Rroxscaffold_1G00060240 Rroxscaffold_1G00060250 Rroxscaffold_1G00060260 Rroxscaffold_1G00060300 Rroxscaffold_1G00060320 Rroxscaffold_1G00060330 Rroxscaffold_1G00060900 Rroxscaffold_1G00060920 Rroxscaffold_1G00073980 Rroxscaffold_2G00077560 Rroxscaffold_2G00077580 Rroxscaffold_2G00146990 Rroxscaffold_3G00236200 Rroxscaffold_3G00236210 Rroxscaffold_3G00236220 Rroxscaffold_4G00292760 Rroxscaffold_4G00315200 Rroxscaffold_4G00315240 Rroxscaffold_4G00326240 Rroxscaffold_4G00328980 Rroxscaffold_5G00341890 Rroxscaffold_7G00161350 Rroxscaffold_7G00174490 Rroxscaffold_7G00206550
rosa_rugosa Rorug01G0093000 Rorug01G0093100 Rorug01G0128800 Rorug01G0312000 Rorug01G0312000 Rorug02G0581100 Rorug03G0224600 Rorug05G0028500 Rorug05G0032700 Rorug05G0032800 Rorug05G0032900 Rorug05G0032900 Rorug05G0033000 Rorug05G0033100 Rorug05G0033300 Rorug05G0033400 Rorug05G0033900 Rorug05G0034900 Rorug05G0035000 Rorug05G0046900 Rorug05G0047500 Rorug05G0047500 Rorug05G0111600 Rorug05G0287900 Rorug05G0288700 Rorug05G0586900 Rorug06G0231700 Rorug06G0354300 Rorug06G0354400
rosa_samantha Rh1DG122200 Rh2DG686800 Rh5BG136600 Rh6AG344500 Rh6BG351900 Rh6CG359000 Rh6CG359100 Rh6CG387600 Rh6CG480500 Rh6DG344600 Rh6DG467400
rosa_wichuraiana Rw0G010250 Rw0G014820 Rw0G021400 Rw0G023100 Rw1G009450 Rw1G012100 Rw1G012180 Rw1G028360 Rw2G054260 Rw3G024370 Rw3G024420 Rw3G024470 Rw5G010850 Rw5G011010 Rw5G011020 Rw5G011050 Rw5G011080 Rw5G011120 Rw5G012300 Rw5G018470 Rw5G033830 Rw6G008930 Rw6G030070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 168
AccI GTMKAC 4 cut(s) 1311, 1321, 1485, 1673
AciI CCGC 4 cut(s) 47, 111, 552, 641
AclWI GGATC 1 cut(s) 2143
AcoI YGGCCR 1 cut(s) 1983
AcsI RAATTY 4 cut(s) 170, 943, 1227, 1877
AcuI CTGAAG 4 cut(s) 279, 1232, 1596, 1701
AfaI GTAC 5 cut(s) 55, 560, 1154, 1210, 2194
AfiI CCNNNNNNNGG 3 cut(s) 525, 552, 818
AflIII ACRYGT 1 cut(s) 2223
AgsI TTSAA 5 cut(s) 658, 680, 1159, 1553, 1788
AjnI CCWGG 4 cut(s) 699, 850, 1764, 2188
AjuI GAANNNNNNNTTGG 2 cut(s) 374, 406
AloI GAACNNNNNNTCC 2 cut(s) 500, 532
Alw21I GWGCWC 4 cut(s) 1521, 1591, 1749, 2127
Alw26I GTCTC 2 cut(s) 1030, 2111
AlwI GGATC 1 cut(s) 2143
AlwNI CAGNNNCTG 1 cut(s) 77
Ama87I CYCGRG 1 cut(s) 1748
AoxI GGCC 4 cut(s) 649, 809, 1983, 2027
ApeKI GCWGC 6 cut(s) 71, 96, 624, 1571, 1580, 1646
ApoI RAATTY 4 cut(s) 170, 943, 1227, 1877
Asp700I GAANNNNTTC 2 cut(s) 584, 1245
AspS9I GGNCC 2 cut(s) 1768, 2186
AsuC2I CCSGG 2 cut(s) 315, 331
AsuHPI GGTGA 7 cut(s) 278, 340, 461, 583, 1733, 1984, 2130
AvaI CYCGRG 1 cut(s) 1748
AvaII GGWCC 2 cut(s) 1768, 2186
BalI TGGCCA 1 cut(s) 1985
BarI GAAGNNNNNNTAC 4 cut(s) 1461, 1493, 1716, 1748
BbsI GAAGAC 1 cut(s) 2226
Bbv12I GWGCWC 4 cut(s) 1521, 1591, 1749, 2127
BbvI GCAGC 6 cut(s) 83, 83, 636, 1583, 1592, 1658
BccI CCATC 6 cut(s) 875, 882, 1027, 1963, 2168, 2240
BceAI ACGGC 2 cut(s) 1009, 1692
BcgI CGANNNNNNTGC 2 cut(s) 472, 506
BciT130I CCWGG 4 cut(s) 701, 852, 1766, 2190
BciVI GTATCC 2 cut(s) 974, 1754
BclI TGATCA 2 cut(s) 451, 2067
BcnI CCSGG 2 cut(s) 315, 331
BcoDI GTCTC 2 cut(s) 1030, 2111
BfaI CTAG 5 cut(s) 51, 83, 1592, 2057, 2208
BfmI CTRYAG 5 cut(s) 622, 825, 1212, 1740, 2094
BfuAI ACCTGC 1 cut(s) 168
BfuI GTATCC 2 cut(s) 974, 1754
BglII AGATCT 1 cut(s) 1008
BisI GCNGC 8 cut(s) 48, 72, 97, 112, 625, 1572, 1581, 1647
BlpI GCTNAGC 1 cut(s) 1839
BlsI GCNGC 8 cut(s) 49, 73, 98, 113, 626, 1573, 1582, 1648
BmcAI AGTACT 2 cut(s) 55, 1154
Bme1390I CCNGG 6 cut(s) 315, 331, 701, 852, 1766, 2190
Bme18I GGWCC 2 cut(s) 1768, 2186
BmeT110I CYCGRG 1 cut(s) 1748
BmgT120I GGNCC 2 cut(s) 1768, 2186
BmiI GGNNCC 3 cut(s) 705, 912, 1770
BmrFI CCNGG 6 cut(s) 315, 331, 701, 852, 1766, 2190
BmrI ACTGGG 1 cut(s) 2211
BmsI GCATC 4 cut(s) 458, 1023, 1899, 2089
BmuI ACTGGG 1 cut(s) 2211
BpiI GAAGAC 1 cut(s) 2226
Bpu1102I GCTNAGC 1 cut(s) 1839
BpuMI CCSGG 2 cut(s) 315, 331
BsaBI GATNNNNATC 1 cut(s) 1013
BsaJI CCNNGG 6 cut(s) 313, 1501, 1905, 2049, 2084, 2151
BsaWI WCCGGW 1 cut(s) 518
BsaXI ACNNNNNCTCC 4 cut(s) 58, 88, 500, 530
Bsc4I CCNNNNNNNGG 3 cut(s) 525, 552, 818
Bse118I RCCGGY 1 cut(s) 1370
Bse1I ACTGG 5 cut(s) 469, 652, 841, 1197, 2206
Bse3DI GCAATG 1 cut(s) 636
Bse8I GATNNNNATC 1 cut(s) 1013
BseBI CCWGG 4 cut(s) 701, 852, 1766, 2190
BseDI CCNNGG 6 cut(s) 313, 1501, 1905, 2049, 2084, 2151
BseGI GGATG 5 cut(s) 326, 473, 607, 874, 2080
BseJI GATNNNNATC 1 cut(s) 1013
BseLI CCNNNNNNNGG 3 cut(s) 525, 552, 818
BseMI GCAATG 1 cut(s) 636
BseMII CTCAG 2 cut(s) 333, 1830
BseNI ACTGG 5 cut(s) 469, 652, 841, 1197, 2206
BseRI GAGGAG 2 cut(s) 161, 1597
BseXI GCAGC 6 cut(s) 83, 83, 636, 1583, 1592, 1658
BseYI CCCAGC 1 cut(s) 548
BsgI GTGCAG 1 cut(s) 1257
BshFI GGCC 4 cut(s) 651, 811, 1985, 2029
BsiHKAI GWGCWC 4 cut(s) 1521, 1591, 1749, 2127
BsiHKCI CYCGRG 1 cut(s) 1748
BsiSI CCGG 4 cut(s) 315, 330, 519, 1371
BslFI GGGAC 1 cut(s) 1765
BslI CCNNNNNNNGG 3 cut(s) 525, 552, 818
BsmAI GTCTC 2 cut(s) 1030, 2111
BsmFI GGGAC 1 cut(s) 1765
BsmI GAATGC 1 cut(s) 1783
BsnI GGCC 4 cut(s) 651, 811, 1985, 2029
BsoBI CYCGRG 1 cut(s) 1748
Bsp1286I GDGCHC 4 cut(s) 1521, 1591, 1749, 2127
Bsp1407I TGTACA 1 cut(s) 558
Bsp143I GATC 7 cut(s) 345, 451, 1008, 1535, 2067, 2098, 2135
Bsp1720I GCTNAGC 1 cut(s) 1839
Bsp19I CCATGG 4 cut(s) 1501, 1905, 2049, 2084
BspACI CCGC 4 cut(s) 47, 111, 552, 641
BspANI GGCC 4 cut(s) 651, 811, 1985, 2029
BspCNI CTCAG 2 cut(s) 334, 1831
BspHI TCATGA 2 cut(s) 1513, 2070
BspLI GGNNCC 3 cut(s) 705, 912, 1770
BspMAI CTGCAG 2 cut(s) 626, 2098
BspMI ACCTGC 1 cut(s) 168
BspPI GGATC 1 cut(s) 2143
BsrDI GCAATG 1 cut(s) 636
BsrFI RCCGGY 1 cut(s) 1370
BsrGI TGTACA 1 cut(s) 558
BsrI ACTGG 5 cut(s) 469, 652, 841, 1197, 2206
BssAI RCCGGY 1 cut(s) 1370
BssECI CCNNGG 6 cut(s) 313, 1501, 1905, 2049, 2084, 2151
BssMI GATC 7 cut(s) 345, 451, 1008, 1535, 2067, 2098, 2135
BssNAI GTATAC 4 cut(s) 1312, 1322, 1486, 1674
BssT1I CCWWGG 5 cut(s) 1501, 1905, 2049, 2084, 2151
Bst1107I GTATAC 4 cut(s) 1312, 1322, 1486, 1674
Bst2UI CCWGG 4 cut(s) 701, 852, 1766, 2190
Bst4CI ACNGT 2 cut(s) 577, 1741
Bst6I CTCTTC 2 cut(s) 1515, 2109
BstAPI GCANNNNNTGC 2 cut(s) 77, 218
BstAUI TGTACA 1 cut(s) 558
BstC8I GCNNGC 7 cut(s) 554, 781, 848, 926, 1372, 1888, 2213
BstDEI CTNAG 3 cut(s) 342, 1839, 1882
BstDSI CCRYGG 4 cut(s) 1501, 1905, 2049, 2084
BstF5I GGATG 5 cut(s) 326, 473, 607, 874, 2080
BstKTI GATC 7 cut(s) 348, 454, 1011, 1538, 2070, 2101, 2138
BstMAI GTCTC 2 cut(s) 1030, 2111
BstMBI GATC 7 cut(s) 345, 451, 1008, 1535, 2067, 2098, 2135
BstNI CCWGG 4 cut(s) 701, 852, 1766, 2190
BstNSI RCATGY 3 cut(s) 475, 2215, 2227
BstSCI CCNGG 6 cut(s) 313, 329, 699, 850, 1764, 2188
BstSFI CTRYAG 5 cut(s) 622, 825, 1212, 1740, 2094
BstV1I GCAGC 6 cut(s) 83, 83, 636, 1583, 1592, 1658
BstV2I GAAGAC 1 cut(s) 2226
BstX2I RGATCY 1 cut(s) 1008
BstXI CCANNNNNNTGG 1 cut(s) 881
BstYI RGATCY 1 cut(s) 1008
BstZ17I GTATAC 4 cut(s) 1312, 1322, 1486, 1674
BsuI GTATCC 2 cut(s) 974, 1754
BsuRI GGCC 4 cut(s) 651, 811, 1985, 2029
BtgI CCRYGG 4 cut(s) 1501, 1905, 2049, 2084
BtsCI GGATG 5 cut(s) 326, 473, 607, 874, 2080
BtsIMutI CAGTG 3 cut(s) 693, 848, 2199
BveI ACCTGC 1 cut(s) 168
Cac8I GCNNGC 7 cut(s) 554, 781, 848, 926, 1372, 1888, 2213
CaiI CAGNNNCTG 1 cut(s) 77
CciI TCATGA 2 cut(s) 1513, 2070
Cfr10I RCCGGY 1 cut(s) 1370
Cfr13I GGNCC 2 cut(s) 1768, 2186
CseI GACGC 1 cut(s) 790
CsiI ACCWGGT 1 cut(s) 699
Csp6I GTAC 5 cut(s) 54, 559, 1153, 1209, 2193
CspCI CAANNNNNGTGG 2 cut(s) 1839, 1874
CviQI GTAC 5 cut(s) 54, 559, 1153, 1209, 2193
DdeI CTNAG 3 cut(s) 342, 1839, 1882
DpnI GATC 7 cut(s) 347, 453, 1010, 1537, 2069, 2100, 2137
DpnII GATC 7 cut(s) 345, 451, 1008, 1535, 2067, 2098, 2135
EaeI YGGCCR 1 cut(s) 1983
Eam1104I CTCTTC 2 cut(s) 1515, 2109
EarI CTCTTC 2 cut(s) 1515, 2109
Eco130I CCWWGG 5 cut(s) 1501, 1905, 2049, 2084, 2151
Eco147I AGGCCT 2 cut(s) 811, 2029
Eco32I GATATC 1 cut(s) 372
Eco47I GGWCC 2 cut(s) 1768, 2186
Eco57I CTGAAG 4 cut(s) 279, 1232, 1596, 1701
Eco88I CYCGRG 1 cut(s) 1748
EcoRI GAATTC 1 cut(s) 170
EcoRII CCWGG 4 cut(s) 699, 850, 1764, 2188
EcoRV GATATC 1 cut(s) 372
EcoT14I CCWWGG 5 cut(s) 1501, 1905, 2049, 2084, 2151
EcoT22I ATGCAT 1 cut(s) 473
ErhI CCWWGG 5 cut(s) 1501, 1905, 2049, 2084, 2151
FalI AAGNNNNNCTT 4 cut(s) 1029, 1061, 2144, 2176
FaqI GGGAC 1 cut(s) 1765
FauI CCCGC 1 cut(s) 545
FauNDI CATATG 2 cut(s) 479, 2239
FbaI TGATCA 2 cut(s) 451, 2067
FblI GTMKAC 4 cut(s) 1311, 1321, 1485, 1673
Fnu4HI GCNGC 8 cut(s) 48, 72, 97, 112, 625, 1572, 1581, 1647
FokI GGATG 5 cut(s) 313, 480, 614, 861, 2067
Fsp4HI GCNGC 8 cut(s) 48, 72, 97, 112, 625, 1572, 1581, 1647
FspBI CTAG 5 cut(s) 51, 83, 1592, 2057, 2208
GluI GCNGC 8 cut(s) 48, 72, 97, 112, 625, 1572, 1581, 1647
GsaI CCCAGC 1 cut(s) 552
HaeIII GGCC 4 cut(s) 651, 811, 1985, 2029
HapII CCGG 4 cut(s) 315, 330, 519, 1371
HgaI GACGC 1 cut(s) 790
HincII GTYRAC 1 cut(s) 805
HindII GTYRAC 1 cut(s) 805
HindIII AAGCTT 1 cut(s) 1442
HinfI GANTC 3 cut(s) 12, 355, 390
HpaII CCGG 4 cut(s) 315, 330, 519, 1371
HphI GGTGA 7 cut(s) 278, 340, 461, 583, 1733, 1984, 2130
Hpy166II GTNNAC 6 cut(s) 805, 1312, 1322, 1486, 1674, 1816
Hpy188I TCNGA 8 cut(s) 298, 343, 376, 601, 1251, 1380, 1690, 2135
Hpy188III TCNNGA 8 cut(s) 878, 1027, 1514, 1634, 1704, 1750, 2071, 2111
Hpy8I GTNNAC 6 cut(s) 805, 1312, 1322, 1486, 1674, 1816
HpyAV CCTTC 6 cut(s) 257, 440, 643, 854, 1246, 1949
HpyCH4III ACNGT 2 cut(s) 577, 1741
HpyCH4IV ACGT 1 cut(s) 1812
HpyF3I CTNAG 3 cut(s) 342, 1839, 1882
HpySE526I ACGT 1 cut(s) 1812
KroI GCCGGC 1 cut(s) 1370
KroNI GCCGGC 1 cut(s) 1372
Ksp22I TGATCA 2 cut(s) 451, 2067
Kzo9I GATC 7 cut(s) 345, 451, 1008, 1535, 2067, 2098, 2135
LmnI GCTCC 5 cut(s) 79, 521, 1586, 1697, 2053
Lsp1109I GCAGC 6 cut(s) 83, 83, 636, 1583, 1592, 1658
LweI GCATC 4 cut(s) 458, 1023, 1899, 2089
MabI ACCWGGT 1 cut(s) 699
MaeI CTAG 5 cut(s) 51, 83, 1592, 2057, 2208
MaeII ACGT 1 cut(s) 1812
MaeIII GTNAC 9 cut(s) 155, 241, 407, 571, 830, 854, 971, 1179, 1808
MalI GATC 7 cut(s) 347, 453, 1010, 1537, 2069, 2100, 2137
MboI GATC 7 cut(s) 345, 451, 1008, 1535, 2067, 2098, 2135
MfeI CAATTG 1 cut(s) 181
MflI RGATCY 1 cut(s) 1008
MhlI GDGCHC 4 cut(s) 1521, 1591, 1749, 2127
MlsI TGGCCA 1 cut(s) 1985
MluNI TGGCCA 1 cut(s) 1985
MlyI GAGTC 1 cut(s) 6
Mox20I TGGCCA 1 cut(s) 1985
Mph1103I ATGCAT 1 cut(s) 473
MroNI GCCGGC 1 cut(s) 1370
MroXI GAANNNNTTC 2 cut(s) 584, 1245
MscI TGGCCA 1 cut(s) 1985
MseI TTAA 6 cut(s) 281, 615, 717, 941, 1847, 1922
MslI CAYNNNNRTG 5 cut(s) 879, 1105, 1431, 1635, 2228
Msp20I TGGCCA 1 cut(s) 1985
MspA1I CMGCKG 2 cut(s) 552, 1574
MspI CCGG 4 cut(s) 315, 330, 519, 1371
MspR9I CCNGG 6 cut(s) 315, 331, 701, 852, 1766, 2190
MunI CAATTG 1 cut(s) 181
Mva1269I GAATGC 1 cut(s) 1783
MvaI CCWGG 4 cut(s) 701, 852, 1766, 2190
NaeI GCCGGC 1 cut(s) 1372
NciI CCSGG 2 cut(s) 315, 331
NcoI CCATGG 4 cut(s) 1501, 1905, 2049, 2084
NdeI CATATG 2 cut(s) 479, 2239
NdeII GATC 7 cut(s) 345, 451, 1008, 1535, 2067, 2098, 2135
NgoMIV GCCGGC 1 cut(s) 1370
NlaIV GGNNCC 3 cut(s) 705, 912, 1770
NmuCI GTSAC 4 cut(s) 407, 571, 971, 1808
NsiI ATGCAT 1 cut(s) 473
NspI RCATGY 3 cut(s) 475, 2215, 2227
PaeI GCATGC 1 cut(s) 2215
PagI TCATGA 2 cut(s) 1513, 2070
PceI AGGCCT 2 cut(s) 811, 2029
PciI ACATGT 1 cut(s) 2223
PcsI WCGNNNNNNNCGW 1 cut(s) 433
PctI GAATGC 1 cut(s) 1783
PdiI GCCGGC 1 cut(s) 1372
PdmI GAANNNNTTC 2 cut(s) 584, 1245
PfeI GAWTC 2 cut(s) 355, 390
PkrI GCNGC 8 cut(s) 49, 73, 98, 113, 626, 1573, 1582, 1648
PleI GAGTC 1 cut(s) 6
PpsI GAGTC 1 cut(s) 6
PscI ACATGT 1 cut(s) 2223
Psp6I CCWGG 4 cut(s) 699, 850, 1764, 2188
PspFI CCCAGC 1 cut(s) 548
PspGI CCWGG 4 cut(s) 699, 850, 1764, 2188
PspN4I GGNNCC 3 cut(s) 705, 912, 1770
PspPI GGNCC 2 cut(s) 1768, 2186
PstI CTGCAG 2 cut(s) 626, 2098
PstNI CAGNNNCTG 1 cut(s) 77
PsuI RGATCY 1 cut(s) 1008
PvuII CAGCTG 1 cut(s) 1574
RsaI GTAC 5 cut(s) 55, 560, 1154, 1210, 2194
RsaNI GTAC 5 cut(s) 54, 559, 1153, 1209, 2193
RseI CAYNNNNRTG 5 cut(s) 879, 1105, 1431, 1635, 2228
SaqAI TTAA 6 cut(s) 281, 615, 717, 941, 1847, 1922
SatI GCNGC 8 cut(s) 48, 72, 97, 112, 625, 1572, 1581, 1647
Sau3AI GATC 7 cut(s) 345, 451, 1008, 1535, 2067, 2098, 2135
Sau96I GGNCC 2 cut(s) 1768, 2186
ScaI AGTACT 2 cut(s) 55, 1154
SchI GAGTC 1 cut(s) 6
ScrFI CCNGG 6 cut(s) 315, 331, 701, 852, 1766, 2190
SduI GDGCHC 4 cut(s) 1521, 1591, 1749, 2127
SexAI ACCWGGT 1 cut(s) 699
SfaNI GCATC 4 cut(s) 458, 1023, 1899, 2089
SfcI CTRYAG 5 cut(s) 622, 825, 1212, 1740, 2094
SinI GGWCC 2 cut(s) 1768, 2186
SmiMI CAYNNNNRTG 5 cut(s) 879, 1105, 1431, 1635, 2228
SphI GCATGC 1 cut(s) 2215
SseBI AGGCCT 2 cut(s) 811, 2029
SsiI CCGC 4 cut(s) 47, 111, 552, 641
SspI AATATT 4 cut(s) 279, 1654, 1963, 2170
SspMI CTAG 5 cut(s) 51, 83, 1592, 2057, 2208
StuI AGGCCT 2 cut(s) 811, 2029
StyD4I CCNGG 6 cut(s) 313, 329, 699, 850, 1764, 2188
StyI CCWWGG 5 cut(s) 1501, 1905, 2049, 2084, 2151
TaaI ACNGT 2 cut(s) 577, 1741
TaiI ACGT 1 cut(s) 1815
TaqI TCGA 4 cut(s) 427, 492, 1028, 1633
TatI WGTACW 3 cut(s) 53, 558, 1152
TauI GCSGC 2 cut(s) 50, 114
TfiI GAWTC 2 cut(s) 355, 390
Tru1I TTAA 6 cut(s) 281, 615, 717, 941, 1847, 1922
Tru9I TTAA 6 cut(s) 281, 615, 717, 941, 1847, 1922
TscAI CASTG 3 cut(s) 700, 848, 2206
TseFI GTSAC 4 cut(s) 407, 571, 971, 1808
TseI GCWGC 6 cut(s) 71, 96, 624, 1571, 1580, 1646
Tsp45I GTSAC 4 cut(s) 407, 571, 971, 1808
TspRI CASTG 3 cut(s) 700, 848, 2206
VpaK11BI GGWCC 2 cut(s) 1768, 2186
XapI RAATTY 4 cut(s) 170, 943, 1227, 1877
XceI RCATGY 3 cut(s) 475, 2215, 2227
XcmI CCANNNNNNNNNTGG 1 cut(s) 848
XmiI GTMKAC 4 cut(s) 1311, 1321, 1485, 1673
XmnI GAANNNNTTC 2 cut(s) 584, 1245
XspI CTAG 5 cut(s) 51, 83, 1592, 2057, 2208
ZrmI AGTACT 2 cut(s) 55, 1154
Zsp2I ATGCAT 1 cut(s) 473
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.