FvH4_7g31880

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0000066
Physical Location & Seq
Reverse (-)
22997630 .. 23001139
3510 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g31880.t1

Sequence Viewer

Length: 2400 bp
ATGGCCTTACATGGGAGAATGCTTCTTTTGCAACTGATCTCTTTGGCCGCAGTACTGCTGTTAGCATCCTCAATGCTAGCAGCTGCTCAATCCCCGCCGCCTGACTGCAAGGAAACCTGTGGTGGCGTCTCAATTCCATATCCATTTGGTCTCACTGACGGTTGTTACCTACATGTGCCGGGACAAGATTCACAGCAATTCAAGCTCGCTTGCGACACCACCGCCACCTCGCAACCGTCCCTAAATTTCTTGGATAGCTATAACTTCCCCACAAACATTTCCAACATTTTCGCGGGTGAGAGTGCGCTGCAAGTAATGTTGACCACGTCCCGAAACTGCTACAACGACACTGTATACAACTCCTCCTTGCACAGAGCCAGTTACCTATATCTTCCGCCTTCTTACACCCTCGCCGACAAAAACAAGGTTTATAACATTGGCTGCAACAAAGCAACGAAGCTCGTAGGTTATCCTCTTCATGTTACAGACCCTGAGAAGCTCTCTGAAGTAGCTTACAGCGGTGTAAGCTTGTGCAACGATGAATTTGGCAAAAGATTCCCCGAGACTTGCACCGGATTCGGATGCACCGTAAATTCTATCCCTAGTGGACTGCAAAATATTACGGTGGATGTGTGGACACTCGGTACCCTCCTCGGAACAACGACGCAGTGGGGACTGAGCTACCCTTGCAGTTACGGCTTCGTTGTGGATGAACGCAACTTCACATTCGCCGGGAATCGAAGTTTTGATGAACTGAATCGCACAAGATTGGAGCTTCCGGTGCTTGCTAGTTGGGCGATTGGGAATGACAACTGCGAAGCAGCAAAGAAGAAGAACGAGACAGCTTTCGCATGCAAGGGTGAGAATACGGAGTGTGTCGACCGCGCTGGTGGTTACTTCTGCCAGTGTTGGGATGGCTACGAAGGGAACCCATACCTCCTCAAAGATTGCGTAGATGTCGATGAGTGCAAGAATAATTCAACCCTTTGCAGTGGTCCTGCAACTTGCGTAAACTCAATTGGAAGTTACAAATGTAAATGTCACAAGGGCTATAGAAATGATGACAACGACAAGAATAAGTGTGTCAAAATCAACGAAACCTCCAGCAAAAATGATACAGAAATGAAAATTTCCTTGGGTGTCTCTTTGAGCTTCCTAGTCATACTGATTATAACTTTTTCGATATACTGCGTACTGAAAAGAAGAAAGTTCAAGCAGCTATGTGACAGGTACTACGAAGTCAATGGGGGCTTCTTGTTACCACAAGAAATGCAAAAGTACAAAGGGTCTCAGGCACCCAGAATATTTAAATTAGCAGAGCTTAACAAGGCAACCAACAAGTTTGATCCCCATGAAATAATTGGAGAAGGAGGCTTTGGATTGGTTTACAAGGGAACATTGCCGGACAACAAGCAGGTGGTTGCTATAAAGAAGTCAAAAACTGATGCTCCCACCATGACCCCCGAAAGTAGGATCGCCCACACTAAGCAGTTCATTAATGAGATGATTGTTCTCTCTGGAATCAAGCATAGAAATGTCGTGACACTCTTAGGTTGTTGTTTGGAGACCAAAACGCCTATATTAGTGTACGAGTTGGTCAGCAATGGCACGCTTTATGAGCACATTCAGAAAAAGAAAGGCAAAGGACCTCTTTCCTTTGGGCAACGAGTGAAGATAGCAGCAGAAACAGCAGGATCCCTGGCATACTTGCACTCCCGCGCTTCTTCCACGCCAATTCTGCACCGAGATGTCAAGGCGACGAACATCCTGCTGGATGAGAATTTGACAGCCAAAGTATCAGACTTTGGAGCTTCAAAATTGGTTCCCGAAGATCAAAATACTCAAATGGCTACTTTAGTGCAAGGGACGCTAGGGTACTTGGACCCTGAATATATTCAGACACACAAACTGACGGAGAAGAGTGATGTCTATAGTTTCGGGGTTGTCCTTGTGGAGCTATTAACGAGTCAAATGGCAATTAATTCCAACAAGAAGCTTGAGGAAGAGAGAAACCTAGCGAACTGTTTTTTAACGGCGGTCGAAGATAATCACTTGGATCAGATTCTTGATCATGAAATTATCAAAGAAGAAAGCTTTGAAATAGCTGAACAAGTAGCCCAACTCGCCCAAAGATGTTTAAGTCGGAAAGGAGGAAATAGGCCTACCATGATAGAAGTAGAAACAGAGCTAGGGGCAATATTGGCAGTTATGGGAAAGCATCCAGGAGGAAAGCCCAACTCCTCCCCCAAAGAGATCGATTACTTGCTTGCAGTGTCACCTTCAAATGCTTTCGTTGTTGATGTTAGAAGTGATGAAGGTGAAGTCATAACCAGCATAGATTATGACAAGAGCATGCATAGTCAAGCCCAGATGATGAAGCCTTATGATGGTGGGAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK

Protein Analysis

800

Amino Acids

88.08

Weight (kDa)

6.2

Isoelectric Point (pI)

35.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 36 - 104 6.2e-08 Wall-associated receptor kinase galacturonan-binding
EGF_CA PF07645 319 - 358 4.4e-09 Calcium-binding EGF domain
Pkinase PF00069 450 - 726 2.2e-48 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 451 - 730 5e-47 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21210 AT1G21230 AT1G21240 AT1G21240 AT1G21245 AT1G21250 AT1G21270 AT1G22720 AT3G25490
fragaria_vesca FvH4_2g35051 FvH4_2g35051 FvH4_2g35060 FvH4_3g10281 FvH4_3g10285 FvH4_3g10286 FvH4_3g10311 FvH4_3g10311 FvH4_3g10320 FvH4_3g10332 FvH4_3g10340 FvH4_3g10350 FvH4_3g10360 FvH4_3g10390 FvH4_3g10412 FvH4_3g10413 FvH4_3g10461 FvH4_3g10462 FvH4_3g10470 FvH4_3g10510 FvH4_3g10520 FvH4_3g10530 FvH4_3g10550 FvH4_3g10560 FvH4_3g10611 FvH4_3g11730 FvH4_6g53140 FvH4_6g53142 FvH4_7g31880 FvH4_7g31910 FvH4_7g31920
malus_domestica MD05G1269900.v1.1 MD05G1270000.v1.1 MD05G1270200.v1.1 MD05G1270400.v1.1 MD05G1270500.v1.1 MD05G1270600.v1.1 MD05G1270800.v1.1 MD05G1270900.v1.1 MD07G1183800.v1.1 MD08G1107600.v1.1 MD10G1248500.v1.1 MD10G1249200.v1.1 MD10G1249500.v1.1 MD10G1250000.v1.1 MD10G1250500.v1.1 MD10G1250900.v1.1 MD10G1251200.v1.1 MD10G1251400.v1.1 MD13G1004600.v1.1 MD15G1088400.v1.1
prunus_persica Prupe.1G442400_v2.0.a1 Prupe.1G442500_v2.0.a1 Prupe.2G223400_v2.0.a1 Prupe.4G091900_v2.0.a1 Prupe.4G093200_v2.0.a1 Prupe.4G093300_v2.0.a1 Prupe.4G093400_v2.0.a1 Prupe.4G093500_v2.0.a1 Prupe.4G094100_v2.0.a1 Prupe.4G094200_v2.0.a1 Prupe.4G094300_v2.0.a1 Prupe.6G358300_v2.0.a1
pyrus_communis pycom08g08990 pycom10g20660 pycom10g20670 pycom10g20680 pycom10g20710 pycom10g20740 pycom13g00400 pycom15g08290
rosa_chinensis RchiOBHm_Chr1g0326861 RchiOBHm_Chr1g0326871 RchiOBHm_Chr1g0331391 RchiOBHm_Chr1g0336911 RchiOBHm_Chr1g0364001 RchiOBHm_Chr2g0174541 RchiOBHm_Chr2g0175041 RchiOBHm_Chr2g0175101 RchiOBHm_Chr2g0175121 RchiOBHm_Chr3g0486331 RchiOBHm_Chr5g0015581 RchiOBHm_Chr5g0016551 RchiOBHm_Chr5g0016561 RchiOBHm_Chr5g0016581 RchiOBHm_Chr5g0016601 RchiOBHm_Chr5g0016611 RchiOBHm_Chr5g0016641 RchiOBHm_Chr5g0016661 RchiOBHm_Chr5g0016691 RchiOBHm_Chr5g0016701 RchiOBHm_Chr5g0016731 RchiOBHm_Chr5g0016741 RchiOBHm_Chr5g0016811 RchiOBHm_Chr5g0016841 RchiOBHm_Chr5g0016871 RchiOBHm_Chr5g0016881 RchiOBHm_Chr5g0016931 RchiOBHm_Chr5g0017021 RchiOBHm_Chr5g0017041 RchiOBHm_Chr5g0017071 RchiOBHm_Chr5g0017091 RchiOBHm_Chr5g0017121 RchiOBHm_Chr5g0017141 RchiOBHm_Chr5g0017291 RchiOBHm_Chr5g0017321 RchiOBHm_Chr5g0017401 RchiOBHm_Chr5g0019001 RchiOBHm_Chr5g0019071 RchiOBHm_Chr5g0028941 RchiOBHm_Chr5g0052141 RchiOBHm_Chr5g0054721 RchiOBHm_Chr5g0054801 RchiOBHm_Chr5g0054931 RchiOBHm_Chr5g0054941 RchiOBHm_Chr6g0259291 RchiOBHm_Chr6g0292991 RchiOBHm_Chr6g0306911
rosa_laevigata RLG00000009465 RLG00000009468 RLG00000010755 RLG00000011976 RLG00000014541 RLG00000014542 RLG00000015169 RLG00000022308 RLG00000027529 RLG00000029324 RLG00000030244 RLG00000030245 RLG00000032210 RLG00000032211 RLG00000032257 RLG00000032259 RLG00000032260 RLG00000032261 RLG00000032262 RLG00000032268 RLG00000032269 RLG00000032272 RLG00000032282 RLG00000032283 RLG00000032308 RLG00000032413 RLG00000033129 RLG00000034945
rosa_multiflora Rmu_co8241929.1_g000001 Rmu_co8269227.1_g000001 Rmu_co8461259.1_g000001 Rmu_co8495385.1_g000001 Rmu_sc0000028.1_g000003 Rmu_sc0000083.1_g000009 Rmu_sc0000083.1_g000010 Rmu_sc0000083.1_g000031 Rmu_sc0000083.1_g000043 Rmu_sc0000083.1_g000046 Rmu_sc0000083.1_g000047 Rmu_sc0000563.1_g000024 Rmu_sc0000842.1_g000012 Rmu_sc0001235.1_g000001 Rmu_sc0001235.1_g000005 Rmu_sc0001235.1_g000009 Rmu_sc0001235.1_g000015 Rmu_sc0001235.1_g000016 Rmu_sc0001235.1_g000017 Rmu_sc0001473.1_g000036 Rmu_sc0001607.1_g000016 Rmu_sc0001788.1_g000006 Rmu_sc0002018.1_g000008 Rmu_sc0002028.1_g000001 Rmu_sc0002680.1_g000011 Rmu_sc0002798.1_g000023 Rmu_sc0002886.1_g000023 Rmu_sc0002989.1_g000012 Rmu_sc0003064.1_g000009 Rmu_sc0003382.1_g000006 Rmu_sc0003630.1_g000002 Rmu_sc0003630.1_g000047 Rmu_sc0003630.1_g000066 Rmu_sc0003630.1_g000067 Rmu_sc0003649.1_g000010 Rmu_sc0003688.1_g000005 Rmu_sc0003920.1_g000007 Rmu_sc0004765.1_g000007 Rmu_sc0004765.1_g000008 Rmu_sc0004920.1_g000011 Rmu_sc0005223.1_g000008 Rmu_sc0005223.1_g000010 Rmu_sc0005223.1_g000015 Rmu_sc0005223.1_g000016 Rmu_sc0005319.1_g000014 Rmu_sc0005319.1_g000015 Rmu_sc0005319.1_g000018 Rmu_sc0005319.1_g000019 Rmu_sc0005319.1_g000025 Rmu_sc0005418.1_g000007 Rmu_sc0005418.1_g000012 Rmu_sc0006106.1_g000011 Rmu_sc0006914.1_g000012 Rmu_sc0007023.1_g000001 Rmu_sc0007662.1_g000001 Rmu_sc0007920.1_g000002 Rmu_sc0007920.1_g000013 Rmu_sc0008053.1_g000016 Rmu_sc0008053.1_g000017 Rmu_sc0008053.1_g000018 Rmu_sc0008053.1_g000023 Rmu_sc0008053.1_g000050 Rmu_sc0009042.1_g000007 Rmu_sc0009042.1_g000008 Rmu_sc0010642.1_g000008 Rmu_sc0011109.1_g000002 Rmu_sc0011314.1_g000006 Rmu_sc0011974.1_g000006 Rmu_sc0012179.1_g000009 Rmu_sc0015169.1_g000001 Rmu_sc0016368.1_g000002 Rmu_sc0022659.1_g000001 Rmu_sc0027371.1_g000001 Rmu_sc0028213.1_g000001 Rmu_sc0034526.1_g000001 Rmu_sc0039366.1_g000002 Rmu_ssc0000050.1_g000084 Rmu_ssc0000050.1_g000088 Rmu_ssc0000167.1_g000017
rosa_roxburghii Rroxscaffold_1G00011340 Rroxscaffold_1G00025710 Rroxscaffold_1G00025870 Rroxscaffold_1G00026070 Rroxscaffold_1G00026170 Rroxscaffold_1G00026200 Rroxscaffold_1G00026270 Rroxscaffold_1G00050950 Rroxscaffold_1G00058900 Rroxscaffold_1G00060040 Rroxscaffold_1G00060060 Rroxscaffold_1G00060070 Rroxscaffold_1G00060080 Rroxscaffold_1G00060110 Rroxscaffold_1G00060120 Rroxscaffold_1G00060140 Rroxscaffold_1G00060170 Rroxscaffold_1G00060210 Rroxscaffold_1G00060220 Rroxscaffold_1G00060240 Rroxscaffold_1G00060250 Rroxscaffold_1G00060260 Rroxscaffold_1G00060300 Rroxscaffold_1G00060320 Rroxscaffold_1G00060330 Rroxscaffold_1G00060900 Rroxscaffold_1G00060920 Rroxscaffold_1G00073980 Rroxscaffold_2G00077560 Rroxscaffold_2G00077580 Rroxscaffold_2G00146990 Rroxscaffold_3G00236200 Rroxscaffold_3G00236210 Rroxscaffold_3G00236220 Rroxscaffold_4G00292760 Rroxscaffold_4G00315200 Rroxscaffold_4G00315240 Rroxscaffold_4G00326240 Rroxscaffold_4G00328980 Rroxscaffold_5G00341890 Rroxscaffold_7G00161350 Rroxscaffold_7G00174490 Rroxscaffold_7G00206550
rosa_rugosa Rorug01G0093000 Rorug01G0093100 Rorug01G0128800 Rorug01G0312000 Rorug01G0312000 Rorug02G0581100 Rorug03G0224600 Rorug05G0028500 Rorug05G0032700 Rorug05G0032800 Rorug05G0032900 Rorug05G0032900 Rorug05G0033000 Rorug05G0033100 Rorug05G0033300 Rorug05G0033400 Rorug05G0033900 Rorug05G0034900 Rorug05G0035000 Rorug05G0046900 Rorug05G0047500 Rorug05G0047500 Rorug05G0111600 Rorug05G0287900 Rorug05G0288700 Rorug05G0586900 Rorug06G0231700 Rorug06G0354300 Rorug06G0354400
rosa_samantha Rh1DG122200 Rh2DG686800 Rh5BG136600 Rh6AG344500 Rh6BG351900 Rh6CG359000 Rh6CG359100 Rh6CG387600 Rh6CG480500 Rh6DG344600 Rh6DG467400
rosa_wichuraiana Rw0G010250 Rw0G014820 Rw0G021400 Rw0G023100 Rw1G009450 Rw1G012100 Rw1G012180 Rw1G028360 Rw2G054260 Rw3G024370 Rw3G024420 Rw3G024470 Rw5G010850 Rw5G011010 Rw5G011020 Rw5G011050 Rw5G011080 Rw5G011120 Rw5G012300 Rw5G018470 Rw5G033830 Rw6G008930 Rw6G030070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 432, 1172
AarI CACCTGC 1 cut(s) 1405
Acc36I ACCTGC 1 cut(s) 1405
Acc65I GGTACC 1 cut(s) 644
AccB1I GGYRCC 2 cut(s) 644, 1294
AccI GTMKAC 2 cut(s) 354, 879
AccII CGCG 3 cut(s) 293, 885, 1719
AclWI GGATC 5 cut(s) 1340, 1481, 1689, 1702, 2064
AcoI YGGCCR 1 cut(s) 45
AcsI RAATTY 5 cut(s) 244, 542, 592, 1128, 1780
AcuI CTGAAG 1 cut(s) 525
AcyI GRCGYC 1 cut(s) 126
AfaI GTAC 7 cut(s) 54, 646, 1194, 1232, 1280, 1589, 1877
AfiI CCNNNNNNNGG 4 cut(s) 12, 910, 1470, 2387
AflIII ACRYGT 1 cut(s) 172
AgsI TTSAA 6 cut(s) 202, 981, 1213, 1815, 2099, 2283
AjiI CACGTC 1 cut(s) 327
AjnI CCWGG 2 cut(s) 1698, 2221
AjuI GAANNNNNNNTTGG 4 cut(s) 1118, 1150, 1359, 1391
Alw21I GWGCWC 1 cut(s) 1623
Alw26I GTCTC 7 cut(s) 133, 155, 557, 833, 1147, 1293, 1559
AlwI GGATC 5 cut(s) 1340, 1481, 1689, 1702, 2064
AlwNI CAGNNNCTG 1 cut(s) 491
Ama87I CYCGRG 1 cut(s) 560
AoxI GGCC 3 cut(s) 3, 45, 2159
ApeKI GCWGC 7 cut(s) 80, 83, 307, 441, 821, 1216, 1679
ApoI RAATTY 5 cut(s) 244, 542, 592, 1128, 1780
AseI ATTAAT 2 cut(s) 1497, 1980
Asp700I GAANNNNTTC 1 cut(s) 1893
Asp718I GGTACC 1 cut(s) 644
AspLEI GCGC 3 cut(s) 307, 887, 1721
AspS9I GGNCC 3 cut(s) 995, 1646, 1882
AsuC2I CCSGG 2 cut(s) 180, 733
AsuHPI GGTGA 4 cut(s) 308, 872, 2268, 2330
AsuNHI GCTAGC 1 cut(s) 76
AvaI CYCGRG 1 cut(s) 560
AvaII GGWCC 3 cut(s) 995, 1646, 1882
BamHI GGATCC 1 cut(s) 1694
BanI GGYRCC 2 cut(s) 644, 1294
Bbv12I GWGCWC 1 cut(s) 1623
BbvI GCAGC 7 cut(s) 70, 92, 294, 428, 833, 1228, 1691
BccI CCATC 2 cut(s) 908, 2381
BceAI ACGGC 2 cut(s) 712, 2049
BcgI CGANNNNNNTGC 4 cut(s) 559, 593, 1750, 1784
BciT130I CCWGG 2 cut(s) 1700, 2223
BclI TGATCA 1 cut(s) 2068
BcnI CCSGG 2 cut(s) 180, 733
BcoDI GTCTC 7 cut(s) 133, 155, 557, 833, 1147, 1293, 1559
BfaI CTAG 7 cut(s) 77, 603, 789, 1157, 1871, 2015, 2189
BfmI CTRYAG 2 cut(s) 1051, 1930
BfuAI ACCTGC 1 cut(s) 1405
BisI GCNGC 9 cut(s) 48, 81, 84, 98, 308, 442, 822, 1217, 1680
BlsI GCNGC 9 cut(s) 49, 82, 85, 99, 309, 443, 823, 1218, 1681
BmcAI AGTACT 1 cut(s) 54
Bme1390I CCNGG 4 cut(s) 180, 733, 1700, 2223
Bme18I GGWCC 3 cut(s) 995, 1646, 1882
BmeT110I CYCGRG 1 cut(s) 560
BmgBI CACGTC 1 cut(s) 327
BmgT120I GGNCC 3 cut(s) 995, 1646, 1882
BmiI GGNNCC 6 cut(s) 646, 929, 1296, 1696, 1824, 1884
BmrFI CCNGG 4 cut(s) 180, 733, 1700, 2223
BmsI GCATC 4 cut(s) 74, 572, 1435, 2227
BmtI GCTAGC 1 cut(s) 80
BplI GAGNNNNNCTC 2 cut(s) 485, 517
BpmI CTGGAG 1 cut(s) 1087
BpuEI CTTGAG 1 cut(s) 2018
BpuMI CCSGG 2 cut(s) 180, 733
Bsa29I ATCGAT 1 cut(s) 2256
BsaHI GRCGYC 1 cut(s) 126
BsaI GGTCTC 3 cut(s) 155, 1293, 1559
BsaJI CCNNGG 3 cut(s) 652, 1134, 1698
BsaWI WCCGGW 2 cut(s) 572, 778
BsaXI ACNNNNNCTCC 8 cut(s) 347, 377, 1085, 1115, 1432, 1462, 1697, 1727
Bsc4I CCNNNNNNNGG 4 cut(s) 12, 910, 1470, 2387
Bse1I ACTGG 2 cut(s) 378, 904
Bse3DI GCAATG 2 cut(s) 1397, 1609
BseBI CCWGG 2 cut(s) 1700, 2223
BseCI ATCGAT 1 cut(s) 2256
BseDI CCNNGG 3 cut(s) 652, 1134, 1698
BseGI GGATG 8 cut(s) 65, 587, 634, 715, 919, 1764, 1780, 2218
BseLI CCNNNNNNNGG 4 cut(s) 12, 910, 1470, 2387
BseMI GCAATG 2 cut(s) 1397, 1609
BseMII CTCAG 3 cut(s) 483, 668, 1304
BseNI ACTGG 2 cut(s) 378, 904
BseRI GAGGAG 4 cut(s) 352, 641, 929, 2230
BseXI GCAGC 7 cut(s) 70, 92, 294, 428, 833, 1228, 1691
BsgI GTGCAG 1 cut(s) 1724
Bsh1236I CGCG 3 cut(s) 293, 885, 1719
Bsh1285I CGRYCG 2 cut(s) 883, 2040
BshFI GGCC 3 cut(s) 5, 47, 2161
BshNI GGYRCC 2 cut(s) 644, 1294
BshVI ATCGAT 1 cut(s) 2256
BsiEI CGRYCG 2 cut(s) 883, 2040
BsiHKAI GWGCWC 1 cut(s) 1623
BsiHKCI CYCGRG 1 cut(s) 560
BsiSI CCGG 5 cut(s) 179, 573, 732, 779, 1403
BslFI GGGAC 5 cut(s) 195, 223, 313, 687, 1879
BslI CCNNNNNNNGG 4 cut(s) 12, 910, 1470, 2387
BsmAI GTCTC 7 cut(s) 133, 155, 557, 833, 1147, 1293, 1559
BsmBI CGTCTC 1 cut(s) 133
BsmFI GGGAC 5 cut(s) 195, 223, 313, 687, 1879
BsmI GAATGC 1 cut(s) 24
BsnI GGCC 3 cut(s) 5, 47, 2161
Bso31I GGTCTC 3 cut(s) 155, 1293, 1559
BsoBI CYCGRG 1 cut(s) 560
Bsp1286I GDGCHC 1 cut(s) 1623
Bsp143I GATC 8 cut(s) 36, 1345, 1473, 1694, 1831, 2056, 2068, 2253
BspANI GGCC 3 cut(s) 5, 47, 2161
BspCNI CTCAG 3 cut(s) 484, 669, 1303
BspDI ATCGAT 1 cut(s) 2256
BspFNI CGCG 3 cut(s) 293, 885, 1719
BspHI TCATGA 1 cut(s) 2071
BspLI GGNNCC 6 cut(s) 646, 929, 1296, 1696, 1824, 1884
BspMI ACCTGC 1 cut(s) 1405
BspOI GCTAGC 1 cut(s) 80
BspPI GGATC 5 cut(s) 1340, 1481, 1689, 1702, 2064
BspT107I GGYRCC 2 cut(s) 644, 1294
BspTNI GGTCTC 3 cut(s) 155, 1293, 1559
BsrDI GCAATG 2 cut(s) 1397, 1609
BsrI ACTGG 2 cut(s) 378, 904
BssECI CCNNGG 3 cut(s) 652, 1134, 1698
BssMI GATC 8 cut(s) 36, 1345, 1473, 1694, 1831, 2056, 2068, 2253
BssNAI GTATAC 1 cut(s) 355
BssNI GRCGYC 1 cut(s) 126
BssT1I CCWWGG 1 cut(s) 1134
Bst1107I GTATAC 1 cut(s) 355
Bst2UI CCWGG 2 cut(s) 1700, 2223
Bst4CI ACNGT 6 cut(s) 161, 237, 352, 589, 625, 2024
Bst6I CTCTTC 3 cut(s) 480, 1913, 1998
BstACI GRCGYC 1 cut(s) 126
BstC8I GCNNGC 8 cut(s) 78, 207, 211, 786, 853, 1610, 2268, 2354
BstDEI CTNAG 5 cut(s) 492, 677, 1290, 1485, 1549
BstF5I GGATG 8 cut(s) 65, 587, 634, 715, 919, 1764, 1780, 2218
BstFNI CGCG 3 cut(s) 293, 885, 1719
BstHHI GCGC 3 cut(s) 307, 887, 1721
BstKTI GATC 8 cut(s) 39, 1348, 1476, 1697, 1834, 2059, 2071, 2256
BstMAI GTCTC 7 cut(s) 133, 155, 557, 833, 1147, 1293, 1559
BstMBI GATC 8 cut(s) 36, 1345, 1473, 1694, 1831, 2056, 2068, 2253
BstMCI CGRYCG 2 cut(s) 883, 2040
BstNI CCWGG 2 cut(s) 1700, 2223
BstNSI RCATGY 3 cut(s) 176, 855, 2356
BstSCI CCNGG 4 cut(s) 178, 731, 1698, 2221
BstSFI CTRYAG 2 cut(s) 1051, 1930
BstUI CGCG 3 cut(s) 293, 885, 1719
BstV1I GCAGC 7 cut(s) 70, 92, 294, 428, 833, 1228, 1691
BstX2I RGATCY 1 cut(s) 1694
BstYI RGATCY 1 cut(s) 1694
BstZ17I GTATAC 1 cut(s) 355
Bsu15I ATCGAT 1 cut(s) 2256
BsuRI GGCC 3 cut(s) 5, 47, 2161
BsuTUI ATCGAT 1 cut(s) 2256
BtrI CACGTC 1 cut(s) 327
BtsCI GGATG 8 cut(s) 65, 587, 634, 715, 919, 1764, 1780, 2218
BtsI GCAGTG 3 cut(s) 674, 997, 2277
BtsIMutI CAGTG 6 cut(s) 153, 348, 674, 911, 997, 2277
BveI ACCTGC 1 cut(s) 1405
Cac8I GCNNGC 8 cut(s) 78, 207, 211, 786, 853, 1610, 2268, 2354
CaiI CAGNNNCTG 1 cut(s) 491
CciI TCATGA 1 cut(s) 2071
CfoI GCGC 3 cut(s) 307, 887, 1721
Cfr13I GGNCC 3 cut(s) 995, 1646, 1882
ClaI ATCGAT 1 cut(s) 2256
CseI GACGC 3 cut(s) 115, 673, 1876
Csp6I GTAC 7 cut(s) 53, 645, 1193, 1231, 1279, 1588, 1876
CviAII CATG 9 cut(s) 11, 173, 479, 852, 1352, 1456, 2072, 2167, 2353
CviQI GTAC 7 cut(s) 53, 645, 1193, 1231, 1279, 1588, 1876
DdeI CTNAG 5 cut(s) 492, 677, 1290, 1485, 1549
DpnI GATC 8 cut(s) 38, 1347, 1475, 1696, 1833, 2058, 2070, 2255
DpnII GATC 8 cut(s) 36, 1345, 1473, 1694, 1831, 2056, 2068, 2253
DraI TTTAAA 1 cut(s) 1309
EaeI YGGCCR 1 cut(s) 45
Eam1104I CTCTTC 3 cut(s) 480, 1913, 1998
EarI CTCTTC 3 cut(s) 480, 1913, 1998
EciI GGCGGA 1 cut(s) 384
Eco130I CCWWGG 1 cut(s) 1134
Eco147I AGGCCT 1 cut(s) 2161
Eco31I GGTCTC 3 cut(s) 155, 1293, 1559
Eco47I GGWCC 3 cut(s) 995, 1646, 1882
Eco57I CTGAAG 1 cut(s) 525
Eco88I CYCGRG 1 cut(s) 560
EcoO109I RGGNCCY 1 cut(s) 1646
EcoRII CCWGG 2 cut(s) 1698, 2221
EcoT14I CCWWGG 1 cut(s) 1134
EcoT22I ATGCAT 1 cut(s) 2358
ErhI CCWWGG 1 cut(s) 1134
Esp3I CGTCTC 1 cut(s) 133
FaeI CATG 9 cut(s) 14, 176, 482, 855, 1355, 1459, 2075, 2170, 2356
FalI AAGNNNNNCTT 2 cut(s) 1635, 1667
FaqI GGGAC 5 cut(s) 195, 223, 313, 687, 1879
FatI CATG 9 cut(s) 10, 172, 478, 851, 1351, 1455, 2071, 2166, 2352
FauI CCCGC 3 cut(s) 102, 286, 1724
FbaI TGATCA 1 cut(s) 2068
FblI GTMKAC 2 cut(s) 354, 879
Fnu4HI GCNGC 9 cut(s) 48, 81, 84, 98, 308, 442, 822, 1217, 1680
FokI GGATG 8 cut(s) 52, 594, 641, 722, 926, 1751, 1787, 2205
Fsp4HI GCNGC 9 cut(s) 48, 81, 84, 98, 308, 442, 822, 1217, 1680
FspBI CTAG 7 cut(s) 77, 603, 789, 1157, 1871, 2015, 2189
GlaI GCGC 3 cut(s) 306, 886, 1720
GluI GCNGC 9 cut(s) 48, 81, 84, 98, 308, 442, 822, 1217, 1680
GsuI CTGGAG 1 cut(s) 1087
HaeIII GGCC 3 cut(s) 5, 47, 2161
HapII CCGG 5 cut(s) 179, 573, 732, 779, 1403
HgaI GACGC 3 cut(s) 115, 673, 1876
HhaI GCGC 3 cut(s) 307, 887, 1721
Hin1I GRCGYC 1 cut(s) 126
Hin1II CATG 9 cut(s) 14, 176, 482, 855, 1355, 1459, 2075, 2170, 2356
Hin6I GCGC 3 cut(s) 305, 885, 1719
HinP1I GCGC 3 cut(s) 305, 885, 1719
HincII GTYRAC 2 cut(s) 321, 880
HindII GTYRAC 2 cut(s) 321, 880
HindIII AAGCTT 3 cut(s) 526, 1994, 2092
HinfI GANTC 8 cut(s) 188, 555, 576, 736, 757, 1521, 1966, 2062
HpaII CCGG 5 cut(s) 179, 573, 732, 779, 1403
HphI GGTGA 4 cut(s) 308, 872, 2268, 2330
Hpy166II GTNNAC 8 cut(s) 321, 355, 608, 636, 880, 1012, 1387, 1588
Hpy188I TCNGA 8 cut(s) 505, 581, 656, 1629, 1801, 1899, 2061, 2145
Hpy188III TCNNGA 6 cut(s) 330, 1518, 1540, 1826, 2066, 2072
Hpy8I GTNNAC 8 cut(s) 321, 355, 608, 636, 880, 1012, 1387, 1588
Hpy99I CGWCG 2 cut(s) 667, 1762
HpyAV CCTTC 5 cut(s) 408, 917, 1361, 2289, 2309
HpyCH4III ACNGT 6 cut(s) 161, 237, 352, 589, 625, 2024
HpyCH4IV ACGT 1 cut(s) 326
HpyF3I CTNAG 5 cut(s) 492, 677, 1290, 1485, 1549
HpySE526I ACGT 1 cut(s) 326
Hsp92I GRCGYC 1 cut(s) 126
Hsp92II CATG 9 cut(s) 14, 176, 482, 855, 1355, 1459, 2075, 2170, 2356
HspAI GCGC 3 cut(s) 305, 885, 1719
KpnI GGTACC 1 cut(s) 648
Ksp22I TGATCA 1 cut(s) 2068
Kzo9I GATC 8 cut(s) 36, 1345, 1473, 1694, 1831, 2056, 2068, 2253
LmnI GCTCC 4 cut(s) 772, 1453, 1808, 1954
Lsp1109I GCAGC 7 cut(s) 70, 92, 294, 428, 833, 1228, 1691
LweI GCATC 4 cut(s) 74, 572, 1435, 2227
MaeI CTAG 7 cut(s) 77, 603, 789, 1157, 1871, 2015, 2189
MaeII ACGT 1 cut(s) 326
MalI GATC 8 cut(s) 38, 1347, 1475, 1696, 1833, 2058, 2070, 2255
MboI GATC 8 cut(s) 36, 1345, 1473, 1694, 1831, 2056, 2068, 2253
MfeI CAATTG 1 cut(s) 1017
MflI RGATCY 1 cut(s) 1694
MhlI GDGCHC 1 cut(s) 1623
MlyI GAGTC 1 cut(s) 1975
MmeI TCCRAC 3 cut(s) 306, 2010, 2123
Mph1103I ATGCAT 1 cut(s) 2358
MroXI GAANNNNTTC 1 cut(s) 1893
MseI TTAA 7 cut(s) 1308, 1323, 1497, 1961, 1980, 2030, 2138
MslI CAYNNNNRTG 2 cut(s) 1533, 1746
MspA1I CMGCKG 2 cut(s) 83, 519
MspI CCGG 5 cut(s) 179, 573, 732, 779, 1403
MspR9I CCNGG 4 cut(s) 180, 733, 1700, 2223
MunI CAATTG 1 cut(s) 1017
Mva1269I GAATGC 1 cut(s) 24
MvaI CCWGG 2 cut(s) 1700, 2223
MvnI CGCG 3 cut(s) 293, 885, 1719
NciI CCSGG 2 cut(s) 180, 733
NdeII GATC 8 cut(s) 36, 1345, 1473, 1694, 1831, 2056, 2068, 2253
NheI GCTAGC 1 cut(s) 76
NlaIII CATG 9 cut(s) 14, 176, 482, 855, 1355, 1459, 2075, 2170, 2356
NlaIV GGNNCC 6 cut(s) 646, 929, 1296, 1696, 1824, 1884
NmuCI GTSAC 4 cut(s) 1040, 1223, 1540, 2274
NsiI ATGCAT 1 cut(s) 2358
NspI RCATGY 3 cut(s) 176, 855, 2356
PaeI GCATGC 2 cut(s) 855, 2356
PagI TCATGA 1 cut(s) 2071
PaqCI CACCTGC 1 cut(s) 1405
PceI AGGCCT 1 cut(s) 2161
PciI ACATGT 1 cut(s) 172
PcsI WCGNNNNNNNCGW 1 cut(s) 585
PctI GAATGC 1 cut(s) 24
PdmI GAANNNNTTC 1 cut(s) 1893
PfeI GAWTC 7 cut(s) 188, 555, 576, 736, 757, 1521, 2062
PflFI GACNNNGTC 1 cut(s) 325
PfoI TCCNGGA 1 cut(s) 2221
PkrI GCNGC 9 cut(s) 49, 82, 85, 99, 309, 443, 823, 1218, 1681
PleI GAGTC 1 cut(s) 1974
PpsI GAGTC 1 cut(s) 1974
PpuMI RGGWCCY 1 cut(s) 1646
PscI ACATGT 1 cut(s) 172
PshBI ATTAAT 2 cut(s) 1497, 1980
PsiI TTATAA 2 cut(s) 432, 1172
Psp5II RGGWCCY 1 cut(s) 1646
Psp6I CCWGG 2 cut(s) 1698, 2221
PspGI CCWGG 2 cut(s) 1698, 2221
PspN4I GGNNCC 6 cut(s) 646, 929, 1296, 1696, 1824, 1884
PspPI GGNCC 3 cut(s) 995, 1646, 1882
PspPPI RGGWCCY 1 cut(s) 1646
PstNI CAGNNNCTG 1 cut(s) 491
PsuI RGATCY 1 cut(s) 1694
PsyI GACNNNGTC 1 cut(s) 325
PvuII CAGCTG 1 cut(s) 83
RsaI GTAC 7 cut(s) 54, 646, 1194, 1232, 1280, 1589, 1877
RsaNI GTAC 7 cut(s) 53, 645, 1193, 1231, 1279, 1588, 1876
RseI CAYNNNNRTG 2 cut(s) 1533, 1746
SalI GTCGAC 1 cut(s) 878
SaqAI TTAA 7 cut(s) 1308, 1323, 1497, 1961, 1980, 2030, 2138
SatI GCNGC 9 cut(s) 48, 81, 84, 98, 308, 442, 822, 1217, 1680
Sau3AI GATC 8 cut(s) 36, 1345, 1473, 1694, 1831, 2056, 2068, 2253
Sau96I GGNCC 3 cut(s) 995, 1646, 1882
ScaI AGTACT 1 cut(s) 54
SchI GAGTC 1 cut(s) 1975
ScrFI CCNGG 4 cut(s) 180, 733, 1700, 2223
SduI GDGCHC 1 cut(s) 1623
SfaNI GCATC 4 cut(s) 74, 572, 1435, 2227
SfcI CTRYAG 2 cut(s) 1051, 1930
SinI GGWCC 3 cut(s) 995, 1646, 1882
SmiI ATTTAAAT 1 cut(s) 1309
SmiMI CAYNNNNRTG 2 cut(s) 1533, 1746
SmlI CTYRAG 1 cut(s) 1997
SmoI CTYRAG 1 cut(s) 1997
SphI GCATGC 2 cut(s) 855, 2356
SseBI AGGCCT 1 cut(s) 2161
SspI AATATT 3 cut(s) 619, 1305, 2199
SspMI CTAG 7 cut(s) 77, 603, 789, 1157, 1871, 2015, 2189
StuI AGGCCT 1 cut(s) 2161
StyD4I CCNGG 4 cut(s) 178, 731, 1698, 2221
StyI CCWWGG 1 cut(s) 1134
SwaI ATTTAAAT 1 cut(s) 1309
TaaI ACNGT 6 cut(s) 161, 237, 352, 589, 625, 2024
TaiI ACGT 1 cut(s) 329
TaqI TCGA 6 cut(s) 739, 879, 960, 1181, 2040, 2256
TatI WGTACW 2 cut(s) 52, 1278
TauI GCSGC 2 cut(s) 50, 100
TfiI GAWTC 7 cut(s) 188, 555, 576, 736, 757, 1521, 2062
Tru1I TTAA 7 cut(s) 1308, 1323, 1497, 1961, 1980, 2030, 2138
Tru9I TTAA 7 cut(s) 1308, 1323, 1497, 1961, 1980, 2030, 2138
TscAI CASTG 6 cut(s) 160, 355, 674, 911, 997, 2277
TseFI GTSAC 4 cut(s) 1040, 1223, 1540, 2274
TseI GCWGC 7 cut(s) 80, 83, 307, 441, 821, 1216, 1679
Tsp45I GTSAC 4 cut(s) 1040, 1223, 1540, 2274
TspGWI ACGGA 2 cut(s) 884, 1928
TspRI CASTG 6 cut(s) 160, 355, 674, 911, 997, 2277
Tth111I GACNNNGTC 1 cut(s) 325
VpaK11BI GGWCC 3 cut(s) 995, 1646, 1882
VspI ATTAAT 2 cut(s) 1497, 1980
XapI RAATTY 5 cut(s) 244, 542, 592, 1128, 1780
XceI RCATGY 3 cut(s) 176, 855, 2356
XcmI CCANNNNNNNNNTGG 2 cut(s) 911, 1358
XmiI GTMKAC 2 cut(s) 354, 879
XmnI GAANNNNTTC 1 cut(s) 1893
XspI CTAG 7 cut(s) 77, 603, 789, 1157, 1871, 2015, 2189
ZrmI AGTACT 1 cut(s) 54
Zsp2I ATGCAT 1 cut(s) 2358
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.