FvH4_2g35060

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000066
Physical Location & Seq
Forward (+)
25921137 .. 25924310
3174 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g35060.t1

Sequence Viewer

Length: 2238 bp
ATGCTTCTCCAACTTCTGCTAATAGGGTCAATACTAGTAGCCACAGCAATTGAAGCACAAGTAGCTGATCAATATGTGAAGCCTGGTTGCCAAGCTACATGTGGAAATGTTAGCATACCATACCCTTTCGGTACCACAGAGGCTTGTTGCCATGATCAAGAGTTCCTTGTAATTTGCAACACCACAGATGATCCACCGAAACTATATCTGACCGGAGGCACAGTTGAGGTCACAAACATTTCTGTTGATGGCAAGGTGGGTATCATGCAATTCATAGCTCATGATTGCTATGAATCATCTGGTTCACCTACAGAGTACAATTCTCCCTGGCTCCACCTCTCCAAATTCATCATCTCCCACACCGATAACTTGTTCATTGCAGTTGGATGTGACACTTATGCTGAAATCCGAGGCTACCAGGGTAACATCAAGAAGTACACAGGTGGCTGCATATCAACATGTGACAGCACTGTCTATGTCACCAATGGTTCTTGTTCCGGTATAGGGTGTTGCCAGACATCAATTGCTAAAGGAGTGAGCTACTTTGATGTGTTTGTGGACAGTTATCTGAATCATACAGATGTTTGGTCTTTCAATCCATGCAGCTACGCCTTTATTGTGGAAGAAAGCAAGTTCAACTTCTCCTTAGCTATGCTCATGGATTTAAAGGATGTAACAGTACTTCCTGTTGTGCTTGATTGGTCAATTGGAAACCAGACTTGCGAGGAAGTACTTGAAGGCAGCGCCTGTCAAGAGAACAGCAGATGTGATAATGTCGAAAATGGATCTGGCTACCAATGCAAATGCAAGGATGGTTATCAAGGGAATCCATACCTCTCTAATGGTTGTCAAGATATTGATGAGTGTGAAAATCCAGAACTAAATAACTGTGTTCAAAGATGTGAAAACACAGAGGGAAGTTATAACTGTCTTTGCCGCAAAGGGTACCATGGCAATGGTATAGAAGGGGAAGAAGGTTGCATAGCTAACAGAACATTGGTTGTTCAAATCACTGTTGGACTTGCTATCGGCCTCATATCGTTGCTTATATGTAGCTCTTGGTTGTATCTGGGCTACAGAAGATGGAAGATGATGAAGCTGAAGGAGAGATTCTTCAGGCAAAATGGAGGATTACTGTTACAGCAACAACTTTCGGTTCGACAAGGTGGTGCCACTACCACTAACCAAACTGCGAAAATCTTTAGCGCAGAAGAACTTGAGAATGCCACCAACAACTACCATGAGACTAGAATTGTTGGTAAAGGAGGCTACGGTACAGTGTACAAAGGAATTTTATCAGATGAAACAGTAGTGGCAATCAAGAAATCCAAAGTAGTTGATCAAAGCCAAATAGAGCAATTCATAAATGAAGTGCTGGTGCTTTCCCAAATCAATCACAGGAATGTGGTGAAGCTTTTGGGTTGTTGTTTCGAGACAGAAGTGCCACTACTAGTGTATGAGTTTGTCACCAATGGTACGCTATTCGACTACATCCACAACAAAAGCAAGGCATGCATCTTTGCTTGGGAAACTCGTTTAAGAATAGCTGCAGAAGCTGCTGGAGTGCTATCATACTTGCACTCTGCAGCTTCTGTTCCTATTATCCATAGAGATGTCAAGTCCACAAACATACTTCTAGATGAAACACTTACAGCAAAAGTGTCTGATTTTGGTGCCTCCAGATTGGTGCCATTGGATCAAGCAGAGCTATCGACAATGGTACAAGGCACGCTCGGGTACTTAGACCCCGAGTACTTGCAAACAAGCCAGTTGACTGACAAGAGCGATGTTTATAGCTTTGGGGTTGTCCTTGTGGAGCTACTAACAGGAAAGAAGGCGCTTTCGTTCGACAAGCCAGAGGAGGAGAGAAATCTAGCCATGTGTTTTCTCTCTGCGCTGAAAGAAGATCGCTTGGTACAAGTCGTTGATGAGAGTGTTCTGAACGGGGCAAATGATGAGCAGCTCAAGGAAGTTTCCAACCTCGCAAAGATGTGTTTGAGAGTAAAAGGAGAAGAAAGACCAACCATGATGGAAGTAGCAAGCGAGCTTGAGGGACTGAGAAGGATGGTAATGCACCATCCATGGGTTAATGATGATCAATACAATGCAGAGGAGAATGAAAACTTGCTTGGTGAAATATCACTGCAAACGTTTAGCCATGGAGGTGGTGGCGATACGAGTTCTGGGTATGATACCATGAAGAACCACATCATATTACCAGTAAATGATGGGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF_CA GUB_WAK_bind Pkinase WAK

Protein Analysis

746

Amino Acids

82.27

Weight (kDa)

4.85

Isoelectric Point (pI)

31.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 30 - 84 1.1e-09 Wall-associated receptor kinase galacturonan-binding
EGF_CA PF07645 285 - 319 1.1e-07 Calcium-binding EGF domain
Pkinase PF00069 413 - 680 2.9e-49 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 416 - 683 4.7e-48 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21210 AT1G21230 AT1G21240 AT1G21240 AT1G21245 AT1G21250 AT1G21270 AT1G22720 AT3G25490
fragaria_vesca FvH4_2g35051 FvH4_2g35051 FvH4_2g35060 FvH4_3g10281 FvH4_3g10285 FvH4_3g10286 FvH4_3g10311 FvH4_3g10311 FvH4_3g10320 FvH4_3g10332 FvH4_3g10340 FvH4_3g10350 FvH4_3g10360 FvH4_3g10390 FvH4_3g10412 FvH4_3g10413 FvH4_3g10461 FvH4_3g10462 FvH4_3g10470 FvH4_3g10510 FvH4_3g10520 FvH4_3g10530 FvH4_3g10550 FvH4_3g10560 FvH4_3g10611 FvH4_3g11730 FvH4_6g53140 FvH4_6g53142 FvH4_7g31880 FvH4_7g31910 FvH4_7g31920
malus_domestica MD05G1269900.v1.1 MD05G1270000.v1.1 MD05G1270200.v1.1 MD05G1270400.v1.1 MD05G1270500.v1.1 MD05G1270600.v1.1 MD05G1270800.v1.1 MD05G1270900.v1.1 MD07G1183800.v1.1 MD08G1107600.v1.1 MD10G1248500.v1.1 MD10G1249200.v1.1 MD10G1249500.v1.1 MD10G1250000.v1.1 MD10G1250500.v1.1 MD10G1250900.v1.1 MD10G1251200.v1.1 MD10G1251400.v1.1 MD13G1004600.v1.1 MD15G1088400.v1.1
prunus_persica Prupe.1G442400_v2.0.a1 Prupe.1G442500_v2.0.a1 Prupe.2G223400_v2.0.a1 Prupe.4G091900_v2.0.a1 Prupe.4G093200_v2.0.a1 Prupe.4G093300_v2.0.a1 Prupe.4G093400_v2.0.a1 Prupe.4G093500_v2.0.a1 Prupe.4G094100_v2.0.a1 Prupe.4G094200_v2.0.a1 Prupe.4G094300_v2.0.a1 Prupe.6G358300_v2.0.a1
pyrus_communis pycom08g08990 pycom10g20660 pycom10g20670 pycom10g20680 pycom10g20710 pycom10g20740 pycom13g00400 pycom15g08290
rosa_chinensis RchiOBHm_Chr1g0326861 RchiOBHm_Chr1g0326871 RchiOBHm_Chr1g0331391 RchiOBHm_Chr1g0336911 RchiOBHm_Chr1g0364001 RchiOBHm_Chr2g0174541 RchiOBHm_Chr2g0175041 RchiOBHm_Chr2g0175101 RchiOBHm_Chr2g0175121 RchiOBHm_Chr3g0486331 RchiOBHm_Chr5g0015581 RchiOBHm_Chr5g0016551 RchiOBHm_Chr5g0016561 RchiOBHm_Chr5g0016581 RchiOBHm_Chr5g0016601 RchiOBHm_Chr5g0016611 RchiOBHm_Chr5g0016641 RchiOBHm_Chr5g0016661 RchiOBHm_Chr5g0016691 RchiOBHm_Chr5g0016701 RchiOBHm_Chr5g0016731 RchiOBHm_Chr5g0016741 RchiOBHm_Chr5g0016811 RchiOBHm_Chr5g0016841 RchiOBHm_Chr5g0016871 RchiOBHm_Chr5g0016881 RchiOBHm_Chr5g0016931 RchiOBHm_Chr5g0017021 RchiOBHm_Chr5g0017041 RchiOBHm_Chr5g0017071 RchiOBHm_Chr5g0017091 RchiOBHm_Chr5g0017121 RchiOBHm_Chr5g0017141 RchiOBHm_Chr5g0017291 RchiOBHm_Chr5g0017321 RchiOBHm_Chr5g0017401 RchiOBHm_Chr5g0019001 RchiOBHm_Chr5g0019071 RchiOBHm_Chr5g0028941 RchiOBHm_Chr5g0052141 RchiOBHm_Chr5g0054721 RchiOBHm_Chr5g0054801 RchiOBHm_Chr5g0054931 RchiOBHm_Chr5g0054941 RchiOBHm_Chr6g0259291 RchiOBHm_Chr6g0292991 RchiOBHm_Chr6g0306911
rosa_laevigata RLG00000009465 RLG00000009468 RLG00000010755 RLG00000011976 RLG00000014541 RLG00000014542 RLG00000015169 RLG00000022308 RLG00000027529 RLG00000029324 RLG00000030244 RLG00000030245 RLG00000032210 RLG00000032211 RLG00000032257 RLG00000032259 RLG00000032260 RLG00000032261 RLG00000032262 RLG00000032268 RLG00000032269 RLG00000032272 RLG00000032282 RLG00000032283 RLG00000032308 RLG00000032413 RLG00000033129 RLG00000034945
rosa_multiflora Rmu_co8241929.1_g000001 Rmu_co8269227.1_g000001 Rmu_co8461259.1_g000001 Rmu_co8495385.1_g000001 Rmu_sc0000028.1_g000003 Rmu_sc0000083.1_g000009 Rmu_sc0000083.1_g000010 Rmu_sc0000083.1_g000031 Rmu_sc0000083.1_g000043 Rmu_sc0000083.1_g000046 Rmu_sc0000083.1_g000047 Rmu_sc0000563.1_g000024 Rmu_sc0000842.1_g000012 Rmu_sc0001235.1_g000001 Rmu_sc0001235.1_g000005 Rmu_sc0001235.1_g000009 Rmu_sc0001235.1_g000015 Rmu_sc0001235.1_g000016 Rmu_sc0001235.1_g000017 Rmu_sc0001473.1_g000036 Rmu_sc0001607.1_g000016 Rmu_sc0001788.1_g000006 Rmu_sc0002018.1_g000008 Rmu_sc0002028.1_g000001 Rmu_sc0002680.1_g000011 Rmu_sc0002798.1_g000023 Rmu_sc0002886.1_g000023 Rmu_sc0002989.1_g000012 Rmu_sc0003064.1_g000009 Rmu_sc0003382.1_g000006 Rmu_sc0003630.1_g000002 Rmu_sc0003630.1_g000047 Rmu_sc0003630.1_g000066 Rmu_sc0003630.1_g000067 Rmu_sc0003649.1_g000010 Rmu_sc0003688.1_g000005 Rmu_sc0003920.1_g000007 Rmu_sc0004765.1_g000007 Rmu_sc0004765.1_g000008 Rmu_sc0004920.1_g000011 Rmu_sc0005223.1_g000008 Rmu_sc0005223.1_g000010 Rmu_sc0005223.1_g000015 Rmu_sc0005223.1_g000016 Rmu_sc0005319.1_g000014 Rmu_sc0005319.1_g000015 Rmu_sc0005319.1_g000018 Rmu_sc0005319.1_g000019 Rmu_sc0005319.1_g000025 Rmu_sc0005418.1_g000007 Rmu_sc0005418.1_g000012 Rmu_sc0006106.1_g000011 Rmu_sc0006914.1_g000012 Rmu_sc0007023.1_g000001 Rmu_sc0007662.1_g000001 Rmu_sc0007920.1_g000002 Rmu_sc0007920.1_g000013 Rmu_sc0008053.1_g000016 Rmu_sc0008053.1_g000017 Rmu_sc0008053.1_g000018 Rmu_sc0008053.1_g000023 Rmu_sc0008053.1_g000050 Rmu_sc0009042.1_g000007 Rmu_sc0009042.1_g000008 Rmu_sc0010642.1_g000008 Rmu_sc0011109.1_g000002 Rmu_sc0011314.1_g000006 Rmu_sc0011974.1_g000006 Rmu_sc0012179.1_g000009 Rmu_sc0015169.1_g000001 Rmu_sc0016368.1_g000002 Rmu_sc0022659.1_g000001 Rmu_sc0027371.1_g000001 Rmu_sc0028213.1_g000001 Rmu_sc0034526.1_g000001 Rmu_sc0039366.1_g000002 Rmu_ssc0000050.1_g000084 Rmu_ssc0000050.1_g000088 Rmu_ssc0000167.1_g000017
rosa_roxburghii Rroxscaffold_1G00011340 Rroxscaffold_1G00025710 Rroxscaffold_1G00025870 Rroxscaffold_1G00026070 Rroxscaffold_1G00026170 Rroxscaffold_1G00026200 Rroxscaffold_1G00026270 Rroxscaffold_1G00050950 Rroxscaffold_1G00058900 Rroxscaffold_1G00060040 Rroxscaffold_1G00060060 Rroxscaffold_1G00060070 Rroxscaffold_1G00060080 Rroxscaffold_1G00060110 Rroxscaffold_1G00060120 Rroxscaffold_1G00060140 Rroxscaffold_1G00060170 Rroxscaffold_1G00060210 Rroxscaffold_1G00060220 Rroxscaffold_1G00060240 Rroxscaffold_1G00060250 Rroxscaffold_1G00060260 Rroxscaffold_1G00060300 Rroxscaffold_1G00060320 Rroxscaffold_1G00060330 Rroxscaffold_1G00060900 Rroxscaffold_1G00060920 Rroxscaffold_1G00073980 Rroxscaffold_2G00077560 Rroxscaffold_2G00077580 Rroxscaffold_2G00146990 Rroxscaffold_3G00236200 Rroxscaffold_3G00236210 Rroxscaffold_3G00236220 Rroxscaffold_4G00292760 Rroxscaffold_4G00315200 Rroxscaffold_4G00315240 Rroxscaffold_4G00326240 Rroxscaffold_4G00328980 Rroxscaffold_5G00341890 Rroxscaffold_7G00161350 Rroxscaffold_7G00174490 Rroxscaffold_7G00206550
rosa_rugosa Rorug01G0093000 Rorug01G0093100 Rorug01G0128800 Rorug01G0312000 Rorug01G0312000 Rorug02G0581100 Rorug03G0224600 Rorug05G0028500 Rorug05G0032700 Rorug05G0032800 Rorug05G0032900 Rorug05G0032900 Rorug05G0033000 Rorug05G0033100 Rorug05G0033300 Rorug05G0033400 Rorug05G0033900 Rorug05G0034900 Rorug05G0035000 Rorug05G0046900 Rorug05G0047500 Rorug05G0047500 Rorug05G0111600 Rorug05G0287900 Rorug05G0288700 Rorug05G0586900 Rorug06G0231700 Rorug06G0354300 Rorug06G0354400
rosa_samantha Rh1DG122200 Rh2DG686800 Rh5BG136600 Rh6AG344500 Rh6BG351900 Rh6CG359000 Rh6CG359100 Rh6CG387600 Rh6CG480500 Rh6DG344600 Rh6DG467400
rosa_wichuraiana Rw0G010250 Rw0G014820 Rw0G021400 Rw0G023100 Rw1G009450 Rw1G012100 Rw1G012180 Rw1G028360 Rw2G054260 Rw3G024370 Rw3G024420 Rw3G024470 Rw5G010850 Rw5G011010 Rw5G011020 Rw5G011050 Rw5G011080 Rw5G011120 Rw5G012300 Rw5G018470 Rw5G033830 Rw6G008930 Rw6G030070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 924
AasI GACNNNNNNGTC 1 cut(s) 470
Acc65I GGTACC 2 cut(s) 131, 945
AccB1I GGYRCC 5 cut(s) 131, 945, 1169, 1673, 1687
AciI CCGC 1 cut(s) 937
AclI AACGTT 1 cut(s) 2150
AclWI GGATC 3 cut(s) 185, 793, 1704
AcsI RAATTY 2 cut(s) 344, 1290
AcuI CTGAAG 2 cut(s) 1099, 1121
AfiI CCNNNNNNNGG 2 cut(s) 504, 2083
AflIII ACRYGT 2 cut(s) 98, 458
AgsI TTSAA 6 cut(s) 53, 595, 637, 737, 896, 1007
AhlI ACTAGT 2 cut(s) 34, 1450
AjnI CCWGG 3 cut(s) 82, 326, 417
AjuI GAANNNNNNNTTGG 2 cut(s) 2112, 2144
Alw26I GTCTC 2 cut(s) 1238, 1427
AlwI GGATC 3 cut(s) 185, 793, 1704
AlwNI CAGNNNCTG 3 cut(s) 747, 1556, 1592
Ama87I CYCGRG 2 cut(s) 1733, 1748
AoxI GGCC 1 cut(s) 1030
ApeKI GCWGC 7 cut(s) 447, 603, 741, 1547, 1556, 1586, 1960
ApoI RAATTY 2 cut(s) 344, 1290
Asp718I GGTACC 2 cut(s) 131, 945
AspLEI GCGC 4 cut(s) 746, 1208, 1840, 1897
AsuHPI GGTGA 5 cut(s) 297, 472, 1420, 1459, 2144
AvaI CYCGRG 2 cut(s) 1733, 1748
BanI GGYRCC 5 cut(s) 131, 945, 1169, 1673, 1687
BarI GAAGNNNNNNTAC 4 cut(s) 666, 698, 1431, 1463
BbvI GCAGC 7 cut(s) 434, 615, 753, 1534, 1543, 1598, 1972
BccI CCATC 7 cut(s) 242, 806, 1077, 2023, 2059, 2085, 2222
BcgI CGANNNNNNTGC 2 cut(s) 1692, 1726
BciT130I CCWGG 3 cut(s) 84, 328, 419
BclI TGATCA 4 cut(s) 67, 154, 1339, 2095
BcoDI GTCTC 2 cut(s) 1238, 1427
BcuI ACTAGT 2 cut(s) 34, 1450
BfaI CTAG 5 cut(s) 35, 1248, 1451, 1637, 1874
BfmI CTRYAG 4 cut(s) 309, 1075, 1548, 1584
BfoI RGCGCY 2 cut(s) 747, 1841
BisI GCNGC 8 cut(s) 448, 604, 742, 937, 1548, 1557, 1587, 1961
BlsI GCNGC 8 cut(s) 449, 605, 743, 938, 1549, 1558, 1588, 1962
BmcAI AGTACT 3 cut(s) 681, 732, 1754
Bme1390I CCNGG 3 cut(s) 84, 328, 419
BmeT110I CYCGRG 2 cut(s) 1733, 1748
BmiI GGNNCC 6 cut(s) 133, 332, 947, 1171, 1675, 1689
BmrFI CCNGG 3 cut(s) 84, 328, 419
BmsI GCATC 1 cut(s) 1524
BpmI CTGGAG 2 cut(s) 1581, 1663
Bpu10I CCTNAGC 1 cut(s) 646
BpuEI CTTGAG 3 cut(s) 1238, 1949, 2069
BsaBI GATNNNNATC 1 cut(s) 816
BsaJI CCNNGG 6 cut(s) 326, 409, 418, 949, 2081, 2158
BsaWI WCCGGW 2 cut(s) 212, 497
BsaXI ACNNNNNCTCC 2 cut(s) 207, 237
Bsc4I CCNNNNNNNGG 2 cut(s) 504, 2083
Bse1I ACTGG 2 cut(s) 1768, 2219
Bse3DI GCAATG 2 cut(s) 375, 961
Bse8I GATNNNNATC 1 cut(s) 816
BseBI CCWGG 3 cut(s) 84, 328, 419
BseDI CCNNGG 6 cut(s) 326, 409, 418, 949, 2081, 2158
BseGI GGATG 6 cut(s) 392, 676, 817, 1491, 2070, 2077
BseJI GATNNNNATC 1 cut(s) 816
BseLI CCNNNNNNNGG 2 cut(s) 504, 2083
BseMI GCAATG 2 cut(s) 375, 961
BseMII CTCAG 1 cut(s) 2048
BseNI ACTGG 2 cut(s) 1768, 2219
BseRI GAGGAG 3 cut(s) 1874, 1877, 2126
BseXI GCAGC 7 cut(s) 434, 615, 753, 1534, 1543, 1598, 1972
BshFI GGCC 1 cut(s) 1032
BshNI GGYRCC 5 cut(s) 131, 945, 1169, 1673, 1687
BsiHKCI CYCGRG 2 cut(s) 1733, 1748
BsiSI CCGG 2 cut(s) 213, 498
BslFI GGGAC 1 cut(s) 2067
BslI CCNNNNNNNGG 2 cut(s) 504, 2083
BsmAI GTCTC 2 cut(s) 1238, 1427
BsmFI GGGAC 1 cut(s) 2067
BsmI GAATGC 1 cut(s) 1228
BsnI GGCC 1 cut(s) 1032
BsoBI CYCGRG 2 cut(s) 1733, 1748
Bsp1407I TGTACA 1 cut(s) 1281
Bsp143I GATC 8 cut(s) 67, 154, 190, 785, 1339, 1696, 1906, 2095
Bsp19I CCATGG 3 cut(s) 949, 2081, 2158
BspACI CCGC 1 cut(s) 937
BspANI GGCC 1 cut(s) 1032
BspCNI CTCAG 1 cut(s) 2049
BspHI TCATGA 1 cut(s) 280
BspLI GGNNCC 6 cut(s) 133, 332, 947, 1171, 1675, 1689
BspMAI CTGCAG 2 cut(s) 1552, 1588
BspPI GGATC 3 cut(s) 185, 793, 1704
BspT107I GGYRCC 5 cut(s) 131, 945, 1169, 1673, 1687
BsrDI GCAATG 2 cut(s) 375, 961
BsrGI TGTACA 1 cut(s) 1281
BsrI ACTGG 2 cut(s) 1768, 2219
BssECI CCNNGG 6 cut(s) 326, 409, 418, 949, 2081, 2158
BssMI GATC 8 cut(s) 67, 154, 190, 785, 1339, 1696, 1906, 2095
BssT1I CCWWGG 3 cut(s) 949, 2081, 2158
Bst2UI CCWGG 3 cut(s) 84, 328, 419
BstAPI GCANNNNNTGC 2 cut(s) 1512, 1556
BstAUI TGTACA 1 cut(s) 1281
BstC8I GCNNGC 4 cut(s) 1513, 1730, 2041, 2045
BstDEI CTNAG 3 cut(s) 646, 1741, 2057
BstDSI CCRYGG 3 cut(s) 949, 2081, 2158
BstF5I GGATG 6 cut(s) 392, 676, 817, 1491, 2070, 2077
BstH2I RGCGCY 2 cut(s) 747, 1841
BstHHI GCGC 4 cut(s) 746, 1208, 1840, 1897
BstKTI GATC 8 cut(s) 70, 157, 193, 788, 1342, 1699, 1909, 2098
BstMAI GTCTC 2 cut(s) 1238, 1427
BstMBI GATC 8 cut(s) 67, 154, 190, 785, 1339, 1696, 1906, 2095
BstMWI GCNNNNNNNGC 6 cut(s) 53, 62, 798, 1512, 1553, 1556
BstNI CCWGG 3 cut(s) 84, 328, 419
BstNSI RCATGY 3 cut(s) 102, 462, 1515
BstSCI CCNGG 3 cut(s) 82, 326, 417
BstSFI CTRYAG 4 cut(s) 309, 1075, 1548, 1584
BstV1I GCAGC 7 cut(s) 434, 615, 753, 1534, 1543, 1598, 1972
BstX2I RGATCY 1 cut(s) 785
BstXI CCANNNNNNTGG 2 cut(s) 956, 2165
BstYI RGATCY 1 cut(s) 785
BsuRI GGCC 1 cut(s) 1032
BtgI CCRYGG 3 cut(s) 949, 2081, 2158
BtgZI GCGATG 1 cut(s) 1800
BtsCI GGATG 6 cut(s) 392, 676, 817, 1491, 2070, 2077
BtsI GCAGTG 1 cut(s) 2141
BtsIMutI CAGTG 4 cut(s) 468, 1011, 1284, 2141
Cac8I GCNNGC 4 cut(s) 1513, 1730, 2041, 2045
CaiI CAGNNNCTG 3 cut(s) 747, 1556, 1592
CciI TCATGA 1 cut(s) 280
CfoI GCGC 4 cut(s) 746, 1208, 1840, 1897
CspCI CAANNNNNGTGG 2 cut(s) 31, 66
DdeI CTNAG 3 cut(s) 646, 1741, 2057
DpnI GATC 8 cut(s) 69, 156, 192, 787, 1341, 1698, 1908, 2097
DpnII GATC 8 cut(s) 67, 154, 190, 785, 1339, 1696, 1906, 2095
DraI TTTAAA 1 cut(s) 666
DrdI GACNNNNNNGTC 1 cut(s) 470
DseDI GACNNNNNNGTC 1 cut(s) 470
Eco130I CCWWGG 3 cut(s) 949, 2081, 2158
Eco57I CTGAAG 2 cut(s) 1099, 1121
Eco88I CYCGRG 2 cut(s) 1733, 1748
EcoRII CCWGG 3 cut(s) 82, 326, 417
EcoT14I CCWWGG 3 cut(s) 949, 2081, 2158
EcoT22I ATGCAT 1 cut(s) 1517
ErhI CCWWGG 3 cut(s) 949, 2081, 2158
FalI AAGNNNNNCTT 4 cut(s) 150, 182, 623, 655
FaqI GGGAC 1 cut(s) 2067
FbaI TGATCA 4 cut(s) 67, 154, 1339, 2095
Fnu4HI GCNGC 8 cut(s) 448, 604, 742, 937, 1548, 1557, 1587, 1961
FokI GGATG 6 cut(s) 399, 683, 824, 1478, 2064, 2077
Fsp4HI GCNGC 8 cut(s) 448, 604, 742, 937, 1548, 1557, 1587, 1961
FspBI CTAG 5 cut(s) 35, 1248, 1451, 1637, 1874
GlaI GCGC 4 cut(s) 745, 1207, 1839, 1896
GluI GCNGC 8 cut(s) 448, 604, 742, 937, 1548, 1557, 1587, 1961
GsuI CTGGAG 2 cut(s) 1581, 1663
HaeII RGCGCY 2 cut(s) 747, 1841
HaeIII GGCC 1 cut(s) 1032
HapII CCGG 2 cut(s) 213, 498
HhaI GCGC 4 cut(s) 746, 1208, 1840, 1897
Hin6I GCGC 4 cut(s) 744, 1206, 1838, 1895
HinP1I GCGC 4 cut(s) 744, 1206, 1838, 1895
HincII GTYRAC 1 cut(s) 1773
HindII GTYRAC 1 cut(s) 1773
HindIII AAGCTT 1 cut(s) 1412
HinfI GANTC 4 cut(s) 293, 571, 826, 1110
HpaII CCGG 2 cut(s) 213, 498
HphI GGTGA 5 cut(s) 297, 472, 1420, 1459, 2144
Hpy166II GTNNAC 6 cut(s) 305, 438, 559, 1282, 1623, 1773
Hpy188I TCNGA 6 cut(s) 210, 410, 570, 1300, 1666, 1941
Hpy8I GTNNAC 6 cut(s) 305, 438, 559, 1282, 1623, 1773
HpyAV CCTTC 6 cut(s) 731, 959, 968, 1096, 1828, 2055
HpyCH4IV ACGT 1 cut(s) 2150
HpyF10VI GCNNNNNNNGC 6 cut(s) 53, 62, 798, 1512, 1553, 1556
HpyF3I CTNAG 3 cut(s) 646, 1741, 2057
HpySE526I ACGT 1 cut(s) 2150
HspAI GCGC 4 cut(s) 744, 1206, 1838, 1895
KpnI GGTACC 2 cut(s) 135, 949
Ksp22I TGATCA 4 cut(s) 67, 154, 1339, 2095
Kzo9I GATC 8 cut(s) 67, 154, 190, 785, 1339, 1696, 1906, 2095
LmnI GCTCC 2 cut(s) 336, 1816
Lsp1109I GCAGC 7 cut(s) 434, 615, 753, 1534, 1543, 1598, 1972
LweI GCATC 1 cut(s) 1524
MaeI CTAG 5 cut(s) 35, 1248, 1451, 1637, 1874
MaeII ACGT 1 cut(s) 2150
MaeIII GTNAC 8 cut(s) 229, 389, 422, 461, 478, 673, 1137, 1465
MalI GATC 8 cut(s) 69, 156, 192, 787, 1341, 1698, 1908, 2097
MboI GATC 8 cut(s) 67, 154, 190, 785, 1339, 1696, 1906, 2095
MboII GAAGA 9 cut(s) 635, 983, 1092, 1099, 1105, 1223, 1916, 2024, 2212
MfeI CAATTG 3 cut(s) 48, 522, 705
MflI RGATCY 1 cut(s) 785
MmeI TCCRAC 4 cut(s) 34, 364, 997, 2001
Mph1103I ATGCAT 1 cut(s) 1517
MseI TTAA 3 cut(s) 665, 1538, 2088
MslI CAYNNNNRTG 5 cut(s) 579, 954, 1401, 1611, 2163
MspI CCGG 2 cut(s) 213, 498
MspR9I CCNGG 3 cut(s) 84, 328, 419
MunI CAATTG 3 cut(s) 48, 522, 705
Mva1269I GAATGC 1 cut(s) 1228
MvaI CCWGG 3 cut(s) 84, 328, 419
MwoI GCNNNNNNNGC 6 cut(s) 53, 62, 798, 1512, 1553, 1556
NcoI CCATGG 3 cut(s) 949, 2081, 2158
NdeII GATC 8 cut(s) 67, 154, 190, 785, 1339, 1696, 1906, 2095
NlaIV GGNNCC 6 cut(s) 133, 332, 947, 1171, 1675, 1689
NmuCI GTSAC 5 cut(s) 229, 389, 461, 478, 1465
NsiI ATGCAT 1 cut(s) 1517
NspI RCATGY 3 cut(s) 102, 462, 1515
PaeI GCATGC 1 cut(s) 1515
PagI TCATGA 1 cut(s) 280
PciI ACATGT 2 cut(s) 98, 458
PctI GAATGC 1 cut(s) 1228
PfeI GAWTC 4 cut(s) 293, 571, 826, 1110
PkrI GCNGC 8 cut(s) 449, 605, 743, 938, 1549, 1558, 1588, 1962
PscI ACATGT 2 cut(s) 98, 458
PsiI TTATAA 1 cut(s) 924
Psp1406I AACGTT 1 cut(s) 2150
Psp6I CCWGG 3 cut(s) 82, 326, 417
PspGI CCWGG 3 cut(s) 82, 326, 417
PspN4I GGNNCC 6 cut(s) 133, 332, 947, 1171, 1675, 1689
PstI CTGCAG 2 cut(s) 1552, 1588
PstNI CAGNNNCTG 3 cut(s) 747, 1556, 1592
PsuI RGATCY 1 cut(s) 785
RseI CAYNNNNRTG 5 cut(s) 579, 954, 1401, 1611, 2163
SaqAI TTAA 3 cut(s) 665, 1538, 2088
SatI GCNGC 8 cut(s) 448, 604, 742, 937, 1548, 1557, 1587, 1961
Sau3AI GATC 8 cut(s) 67, 154, 190, 785, 1339, 1696, 1906, 2095
ScaI AGTACT 3 cut(s) 681, 732, 1754
ScrFI CCNGG 3 cut(s) 84, 328, 419
SfaNI GCATC 1 cut(s) 1524
SfcI CTRYAG 4 cut(s) 309, 1075, 1548, 1584
SmiMI CAYNNNNRTG 5 cut(s) 579, 954, 1401, 1611, 2163
SmlI CTYRAG 3 cut(s) 1217, 1964, 2048
SmoI CTYRAG 3 cut(s) 1217, 1964, 2048
SpeI ACTAGT 2 cut(s) 34, 1450
SphI GCATGC 1 cut(s) 1515
SsiI CCGC 1 cut(s) 937
SspMI CTAG 5 cut(s) 35, 1248, 1451, 1637, 1874
StyD4I CCNGG 3 cut(s) 82, 326, 417
StyI CCWWGG 3 cut(s) 949, 2081, 2158
TaiI ACGT 1 cut(s) 2153
TaqI TCGA 6 cut(s) 777, 1159, 1431, 1485, 1712, 1848
TatI WGTACW 6 cut(s) 315, 435, 679, 730, 1281, 1752
TauI GCSGC 1 cut(s) 939
TfiI GAWTC 4 cut(s) 293, 571, 826, 1110
Tru1I TTAA 3 cut(s) 665, 1538, 2088
Tru9I TTAA 3 cut(s) 665, 1538, 2088
TscAI CASTG 4 cut(s) 475, 1018, 1284, 2148
TseFI GTSAC 5 cut(s) 229, 389, 461, 478, 1465
TseI GCWGC 7 cut(s) 447, 603, 741, 1547, 1556, 1586, 1960
Tsp45I GTSAC 5 cut(s) 229, 389, 461, 478, 1465
TspRI CASTG 4 cut(s) 475, 1018, 1284, 2148
XapI RAATTY 2 cut(s) 344, 1290
XbaI TCTAGA 1 cut(s) 1636
XceI RCATGY 3 cut(s) 102, 462, 1515
XcmI CCANNNNNNNNNTGG 2 cut(s) 98, 2165
XspI CTAG 5 cut(s) 35, 1248, 1451, 1637, 1874
ZrmI AGTACT 3 cut(s) 681, 732, 1754
Zsp2I ATGCAT 1 cut(s) 1517
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.