FvH4_3g10611

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000066
Physical Location & Seq
Reverse (-)
6324483 .. 6328427
3945 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g10611.t1

Sequence Viewer

Length: 2316 bp
ATGATGGCCTTGGTACCTGAGAAGATGCTTACTATTATGCAGCTCTATTTCATAGCGGCTGCAACGATCACAATTCTTCTAGCTGCAGAGTCCACAGCGCTTCCTCAAGCCAAGCCAGGATGCCAAGAGAAGTGCGGTGATCTCATAATCCCATACCCGTTTGGCGTAGGCGAATTCTGTTCGATGCGACCAGAGTTCACCGTTACTTGCGATCAATCCACTACAGCAGCACCCTCATCAGCAAACTTTACAAACAGTACCTTCACCTTTCCCATCACCATTTCTAACTTTTCTCTTGTTGACGGTGAGTTACAAGTACTGCAGGCCATAGCCCGAGATTGTTTTGACGAGGAGGGTAATACTACAAACATCGGGAGTGTGCTACAGCTACCTCCTTCTTACACCATATCTACCAGAAACGACTTCTTCATCATTGGCTGTAACGTTTTAGGAGTCTTTGGGGAAGGTGTGGATGTGGGATACGAAACAGGAATAAATTCGGCCTTCTGTATGGATTTTCTTCCCAATTTGACCAAGTCTTGCTCCGGCGTTGGGTGCTCCAGAACTACCGTCCCCGGTGGAATGCAAAATGTTAGTATTCTCATGGAGAAAATGAACGACTTTAACGCAACCGACCTGTGGCAATCAAACTACCCGTGCAGCTACGCCTTCATTGTGAAAGACGGAAATTTCACGTTCGACCCGGAATCAAGTTTTCAGCAACTGAAAACCACAAGGCAGCAGCTTCCAGTGGCTGTGAATTGGGCTGTCGGGGATAAGTCTTGTGAAGAAGCAACTCATTTATGCGCAGGAAATGCGTCCTGTGTCGACCGAAGTACCATCACCGATGGGATGGTTGGTTATATTTGCCGGTGCTGGCCAGGTTACGAAGGAAATCCTTATCTCGGTTGCCAAGATATTGACGAGTGCACCGCTTTAGTACCGGGACGCTGTGAGTATGGGAGGTGCGTAAATTCACCTCCAGGAAATTACTCATGCGAGTGCAGTAAAGGATTTAAAAACCTAGACGCCATGACTTGTATACCCAATTCCTCGTCAAACAACACTACTTCCTGGAAAATTTCATTGGGTTTCAGTATAGGCTTCTTGGTTTTAATGGTTTTAATCTTGTGGATTTATCGGGGAGTGAACAAAAGGAGACTCGTGAGACTCAAAAAAAAGAACTTCAGAGAAAACGGCGGCCCAACACTACAACAACAGCTCGCTAGTCATGGAGGATCTGTTGAGACAGCAAAAATCTTTACTGCGGAGGAACTTAAGAAAGCCACAAACAATTACCATGTAGATGAAATCCTTGGAGAAGGAGGCTATGGAACAGTTTACAAAGGGACGCTACATGATAAGAATGTTGTTGCCATAAAGAAGCCAAAGATTGGCGCTCCAACTGAAAGTGATCAGTTCGTTAATGAGGTTATCGTTCTTTCTCAAATCAAACACAGAAATATAGTGAAACTATTAGGTTGTTGTTTCGAGACCGAAGTGCCTTTATTAGTATACGAGTACATCACTCATGGTACTCTTTTCGAGCATCTATTCAAAAATGGAAAAGGATATTCCTCGCTTTCATGGGAATTACGATTGAAGATAGCATCAGAAACGGCAGGGGCATTAGCATACTTACACTCCTCTACTTCCACTCCAATCATACATCGAGACGTGAAATCTATGAATATATTATTGGATGACAAATACACTGCCAAAGTGTCTGATTTCGGAGCTTCACGTTTCATTCCTCTAGATCAAACTCAACTTGCAACACTAGTTCAAGGGACGCTTGGGTACTTGGATCCTGAATACTTTCATTCAAACCAGCTGACCGAAAAGAGCGACGTCTATAGCTTTGGAGTTGTCCTAGCGGAGCTACTAACAAGTAGAGTTGCACTTTCTTTTGCTAGGCCGGACAAGGAGAGATGTCTAGCAAGCTTCTTTGTTTCTTCAATTGAAAACGATCGCTTGATTCAGATTCTTGATGCTGATATGGTGAATGAGGGGAACATAGAGACAGTAGAGCAAGTAGCGAGACTGGCAAAAAGATGTTTGATGATAAAAGGCGACGAAAGGCCTACGATGAGGGAAGTTGCCATGGAGTTAGAAGGGATGAGAATTACGGCAAAGCATACATGGAAAAATGGCGATTCCGGCTTCGAGGAGACTGAGTACTTGCTCGGGTCACATGATTCAGCTGCTTCCGTGGTGAATATTGGAGGCGGAGGTGACGGTGGTTCGAGCGTTACAAATACTGGTTATGACAGCATGCAAATTCAAATGTTAATGCCCTACGATGACGGGCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK hEGF

Protein Analysis

772

Amino Acids

84.62

Weight (kDa)

5.16

Isoelectric Point (pI)

32.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 41 - 77 1.8e-07 Wall-associated receptor kinase galacturonan-binding
Pkinase PF00069 433 - 700 2.3e-45 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 435 - 704 1.5e-47 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21210 AT1G21230 AT1G21240 AT1G21240 AT1G21245 AT1G21250 AT1G21270 AT1G22720 AT3G25490
fragaria_vesca FvH4_2g35051 FvH4_2g35051 FvH4_2g35060 FvH4_3g10281 FvH4_3g10285 FvH4_3g10286 FvH4_3g10311 FvH4_3g10311 FvH4_3g10320 FvH4_3g10332 FvH4_3g10340 FvH4_3g10350 FvH4_3g10360 FvH4_3g10390 FvH4_3g10412 FvH4_3g10413 FvH4_3g10461 FvH4_3g10462 FvH4_3g10470 FvH4_3g10510 FvH4_3g10520 FvH4_3g10530 FvH4_3g10550 FvH4_3g10560 FvH4_3g10611 FvH4_3g11730 FvH4_6g53140 FvH4_6g53142 FvH4_7g31880 FvH4_7g31910 FvH4_7g31920
malus_domestica MD05G1269900.v1.1 MD05G1270000.v1.1 MD05G1270200.v1.1 MD05G1270400.v1.1 MD05G1270500.v1.1 MD05G1270600.v1.1 MD05G1270800.v1.1 MD05G1270900.v1.1 MD07G1183800.v1.1 MD08G1107600.v1.1 MD10G1248500.v1.1 MD10G1249200.v1.1 MD10G1249500.v1.1 MD10G1250000.v1.1 MD10G1250500.v1.1 MD10G1250900.v1.1 MD10G1251200.v1.1 MD10G1251400.v1.1 MD13G1004600.v1.1 MD15G1088400.v1.1
prunus_persica Prupe.1G442400_v2.0.a1 Prupe.1G442500_v2.0.a1 Prupe.2G223400_v2.0.a1 Prupe.4G091900_v2.0.a1 Prupe.4G093200_v2.0.a1 Prupe.4G093300_v2.0.a1 Prupe.4G093400_v2.0.a1 Prupe.4G093500_v2.0.a1 Prupe.4G094100_v2.0.a1 Prupe.4G094200_v2.0.a1 Prupe.4G094300_v2.0.a1 Prupe.6G358300_v2.0.a1
pyrus_communis pycom08g08990 pycom10g20660 pycom10g20670 pycom10g20680 pycom10g20710 pycom10g20740 pycom13g00400 pycom15g08290
rosa_chinensis RchiOBHm_Chr1g0326861 RchiOBHm_Chr1g0326871 RchiOBHm_Chr1g0331391 RchiOBHm_Chr1g0336911 RchiOBHm_Chr1g0364001 RchiOBHm_Chr2g0174541 RchiOBHm_Chr2g0175041 RchiOBHm_Chr2g0175101 RchiOBHm_Chr2g0175121 RchiOBHm_Chr3g0486331 RchiOBHm_Chr5g0015581 RchiOBHm_Chr5g0016551 RchiOBHm_Chr5g0016561 RchiOBHm_Chr5g0016581 RchiOBHm_Chr5g0016601 RchiOBHm_Chr5g0016611 RchiOBHm_Chr5g0016641 RchiOBHm_Chr5g0016661 RchiOBHm_Chr5g0016691 RchiOBHm_Chr5g0016701 RchiOBHm_Chr5g0016731 RchiOBHm_Chr5g0016741 RchiOBHm_Chr5g0016811 RchiOBHm_Chr5g0016841 RchiOBHm_Chr5g0016871 RchiOBHm_Chr5g0016881 RchiOBHm_Chr5g0016931 RchiOBHm_Chr5g0017021 RchiOBHm_Chr5g0017041 RchiOBHm_Chr5g0017071 RchiOBHm_Chr5g0017091 RchiOBHm_Chr5g0017121 RchiOBHm_Chr5g0017141 RchiOBHm_Chr5g0017291 RchiOBHm_Chr5g0017321 RchiOBHm_Chr5g0017401 RchiOBHm_Chr5g0019001 RchiOBHm_Chr5g0019071 RchiOBHm_Chr5g0028941 RchiOBHm_Chr5g0052141 RchiOBHm_Chr5g0054721 RchiOBHm_Chr5g0054801 RchiOBHm_Chr5g0054931 RchiOBHm_Chr5g0054941 RchiOBHm_Chr6g0259291 RchiOBHm_Chr6g0292991 RchiOBHm_Chr6g0306911
rosa_laevigata RLG00000009465 RLG00000009468 RLG00000010755 RLG00000011976 RLG00000014541 RLG00000014542 RLG00000015169 RLG00000022308 RLG00000027529 RLG00000029324 RLG00000030244 RLG00000030245 RLG00000032210 RLG00000032211 RLG00000032257 RLG00000032259 RLG00000032260 RLG00000032261 RLG00000032262 RLG00000032268 RLG00000032269 RLG00000032272 RLG00000032282 RLG00000032283 RLG00000032308 RLG00000032413 RLG00000033129 RLG00000034945
rosa_multiflora Rmu_co8241929.1_g000001 Rmu_co8269227.1_g000001 Rmu_co8461259.1_g000001 Rmu_co8495385.1_g000001 Rmu_sc0000028.1_g000003 Rmu_sc0000083.1_g000009 Rmu_sc0000083.1_g000010 Rmu_sc0000083.1_g000031 Rmu_sc0000083.1_g000043 Rmu_sc0000083.1_g000046 Rmu_sc0000083.1_g000047 Rmu_sc0000563.1_g000024 Rmu_sc0000842.1_g000012 Rmu_sc0001235.1_g000001 Rmu_sc0001235.1_g000005 Rmu_sc0001235.1_g000009 Rmu_sc0001235.1_g000015 Rmu_sc0001235.1_g000016 Rmu_sc0001235.1_g000017 Rmu_sc0001473.1_g000036 Rmu_sc0001607.1_g000016 Rmu_sc0001788.1_g000006 Rmu_sc0002018.1_g000008 Rmu_sc0002028.1_g000001 Rmu_sc0002680.1_g000011 Rmu_sc0002798.1_g000023 Rmu_sc0002886.1_g000023 Rmu_sc0002989.1_g000012 Rmu_sc0003064.1_g000009 Rmu_sc0003382.1_g000006 Rmu_sc0003630.1_g000002 Rmu_sc0003630.1_g000047 Rmu_sc0003630.1_g000066 Rmu_sc0003630.1_g000067 Rmu_sc0003649.1_g000010 Rmu_sc0003688.1_g000005 Rmu_sc0003920.1_g000007 Rmu_sc0004765.1_g000007 Rmu_sc0004765.1_g000008 Rmu_sc0004920.1_g000011 Rmu_sc0005223.1_g000008 Rmu_sc0005223.1_g000010 Rmu_sc0005223.1_g000015 Rmu_sc0005223.1_g000016 Rmu_sc0005319.1_g000014 Rmu_sc0005319.1_g000015 Rmu_sc0005319.1_g000018 Rmu_sc0005319.1_g000019 Rmu_sc0005319.1_g000025 Rmu_sc0005418.1_g000007 Rmu_sc0005418.1_g000012 Rmu_sc0006106.1_g000011 Rmu_sc0006914.1_g000012 Rmu_sc0007023.1_g000001 Rmu_sc0007662.1_g000001 Rmu_sc0007920.1_g000002 Rmu_sc0007920.1_g000013 Rmu_sc0008053.1_g000016 Rmu_sc0008053.1_g000017 Rmu_sc0008053.1_g000018 Rmu_sc0008053.1_g000023 Rmu_sc0008053.1_g000050 Rmu_sc0009042.1_g000007 Rmu_sc0009042.1_g000008 Rmu_sc0010642.1_g000008 Rmu_sc0011109.1_g000002 Rmu_sc0011314.1_g000006 Rmu_sc0011974.1_g000006 Rmu_sc0012179.1_g000009 Rmu_sc0015169.1_g000001 Rmu_sc0016368.1_g000002 Rmu_sc0022659.1_g000001 Rmu_sc0027371.1_g000001 Rmu_sc0028213.1_g000001 Rmu_sc0034526.1_g000001 Rmu_sc0039366.1_g000002 Rmu_ssc0000050.1_g000084 Rmu_ssc0000050.1_g000088 Rmu_ssc0000167.1_g000017
rosa_roxburghii Rroxscaffold_1G00011340 Rroxscaffold_1G00025710 Rroxscaffold_1G00025870 Rroxscaffold_1G00026070 Rroxscaffold_1G00026170 Rroxscaffold_1G00026200 Rroxscaffold_1G00026270 Rroxscaffold_1G00050950 Rroxscaffold_1G00058900 Rroxscaffold_1G00060040 Rroxscaffold_1G00060060 Rroxscaffold_1G00060070 Rroxscaffold_1G00060080 Rroxscaffold_1G00060110 Rroxscaffold_1G00060120 Rroxscaffold_1G00060140 Rroxscaffold_1G00060170 Rroxscaffold_1G00060210 Rroxscaffold_1G00060220 Rroxscaffold_1G00060240 Rroxscaffold_1G00060250 Rroxscaffold_1G00060260 Rroxscaffold_1G00060300 Rroxscaffold_1G00060320 Rroxscaffold_1G00060330 Rroxscaffold_1G00060900 Rroxscaffold_1G00060920 Rroxscaffold_1G00073980 Rroxscaffold_2G00077560 Rroxscaffold_2G00077580 Rroxscaffold_2G00146990 Rroxscaffold_3G00236200 Rroxscaffold_3G00236210 Rroxscaffold_3G00236220 Rroxscaffold_4G00292760 Rroxscaffold_4G00315200 Rroxscaffold_4G00315240 Rroxscaffold_4G00326240 Rroxscaffold_4G00328980 Rroxscaffold_5G00341890 Rroxscaffold_7G00161350 Rroxscaffold_7G00174490 Rroxscaffold_7G00206550
rosa_rugosa Rorug01G0093000 Rorug01G0093100 Rorug01G0128800 Rorug01G0312000 Rorug01G0312000 Rorug02G0581100 Rorug03G0224600 Rorug05G0028500 Rorug05G0032700 Rorug05G0032800 Rorug05G0032900 Rorug05G0032900 Rorug05G0033000 Rorug05G0033100 Rorug05G0033300 Rorug05G0033400 Rorug05G0033900 Rorug05G0034900 Rorug05G0035000 Rorug05G0046900 Rorug05G0047500 Rorug05G0047500 Rorug05G0111600 Rorug05G0287900 Rorug05G0288700 Rorug05G0586900 Rorug06G0231700 Rorug06G0354300 Rorug06G0354400
rosa_samantha Rh1DG122200 Rh2DG686800 Rh5BG136600 Rh6AG344500 Rh6BG351900 Rh6CG359000 Rh6CG359100 Rh6CG387600 Rh6CG480500 Rh6DG344600 Rh6DG467400
rosa_wichuraiana Rw0G010250 Rw0G014820 Rw0G021400 Rw0G023100 Rw1G009450 Rw1G012100 Rw1G012180 Rw1G028360 Rw2G054260 Rw3G024370 Rw3G024420 Rw3G024470 Rw5G010850 Rw5G011010 Rw5G011020 Rw5G011050 Rw5G011080 Rw5G011120 Rw5G012300 Rw5G018470 Rw5G033830 Rw6G008930 Rw6G030070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1854
Acc16I TGCGCA 1 cut(s) 808
Acc65I GGTACC 1 cut(s) 13
AccB1I GGYRCC 1 cut(s) 13
AccB7I CCANNNNNTGG 1 cut(s) 1394
AccI GTMKAC 3 cut(s) 828, 1042, 1515
AciI CCGC 7 cut(s) 56, 135, 933, 1200, 1268, 1877, 2229
AclI AACGTT 1 cut(s) 444
AclWI GGATC 3 cut(s) 1246, 1802, 1815
AcoI YGGCCR 1 cut(s) 878
AcsI RAATTY 6 cut(s) 173, 496, 688, 973, 1080, 2280
AcuI CTGAAG 1 cut(s) 1171
AcyI GRCGYC 2 cut(s) 1029, 1851
AfaI GTAC 9 cut(s) 15, 259, 318, 838, 942, 1523, 1537, 1802, 2180
AfeI AGCGCT 1 cut(s) 99
AfiI CCNNNNNNNGG 4 cut(s) 552, 639, 905, 1394
AflII CTTAAG 1 cut(s) 1277
AgsI TTSAA 7 cut(s) 1558, 1603, 1787, 1827, 1959, 1964, 2285
AhlI ACTAGT 1 cut(s) 1780
AjiI CACGTC 1 cut(s) 1678
AjnI CCWGG 4 cut(s) 115, 880, 982, 1073
AjuI GAANNNNNNNTTGG 4 cut(s) 1070, 1102, 1682, 1714
Alw21I GWGCWC 2 cut(s) 560, 932
Alw26I GTCTC 8 cut(s) 1153, 1162, 1241, 1487, 1668, 2015, 2035, 2165
Alw44I GTGCAC 1 cut(s) 928
AlwI GGATC 3 cut(s) 1246, 1802, 1815
AlwNI CAGNNNCTG 2 cut(s) 724, 755
Ama87I CYCGRG 2 cut(s) 333, 2186
Aor51HI AGCGCT 1 cut(s) 99
AoxI GGCC 7 cut(s) 6, 324, 501, 878, 1201, 1916, 2081
ApaLI GTGCAC 1 cut(s) 928
ApeKI GCWGC 8 cut(s) 40, 59, 83, 227, 660, 739, 742, 2204
ApoI RAATTY 6 cut(s) 173, 496, 688, 973, 1080, 2280
Asp700I GAANNNNTTC 2 cut(s) 496, 1818
Asp718I GGTACC 1 cut(s) 13
AspLEI GCGC 3 cut(s) 100, 809, 1400
AspS9I GGNCC 1 cut(s) 1202
AsuC2I CCSGG 3 cut(s) 576, 704, 945
AvaI CYCGRG 2 cut(s) 333, 2186
BaeGI GKGCMC 1 cut(s) 932
BaeI ACNNNNGTAYC 2 cut(s) 820, 853
BalI TGGCCA 1 cut(s) 880
BamHI GGATCC 1 cut(s) 1807
BanI GGYRCC 1 cut(s) 13
BauI CACGAG 1 cut(s) 1163
Bbv12I GWGCWC 2 cut(s) 560, 932
BbvI GCAGC 8 cut(s) 46, 52, 70, 239, 672, 751, 754, 2191
BccI CCATC 4 cut(s) 281, 842, 847, 848
BceAI ACGGC 3 cut(s) 1213, 1635, 2145
BciT130I CCWGG 4 cut(s) 117, 882, 984, 1075
BciVI GTATCC 1 cut(s) 473
BclI TGATCA 1 cut(s) 1414
BcnI CCSGG 3 cut(s) 576, 704, 945
BcoDI GTCTC 8 cut(s) 1153, 1162, 1241, 1487, 1668, 2015, 2035, 2165
BcuI ACTAGT 1 cut(s) 1780
BfaI CTAG 8 cut(s) 80, 1025, 1227, 1757, 1781, 1874, 1914, 1937
BfmI CTRYAG 5 cut(s) 84, 222, 320, 383, 1855
BfoI RGCGCY 2 cut(s) 101, 1401
BfrI CTTAAG 1 cut(s) 1277
BfuI GTATCC 1 cut(s) 473
BmcAI AGTACT 2 cut(s) 318, 2180
Bme1390I CCNGG 7 cut(s) 117, 576, 704, 882, 945, 984, 1075
BmeT110I CYCGRG 2 cut(s) 333, 2186
BmgBI CACGTC 1 cut(s) 1678
BmgT120I GGNCC 1 cut(s) 1202
BmiI GGNNCC 2 cut(s) 15, 1809
BmrFI CCNGG 7 cut(s) 117, 576, 704, 882, 945, 984, 1075
BmsI GCATC 6 cut(s) 15, 110, 174, 1558, 1619, 1981
BpmI CTGGAG 2 cut(s) 544, 966
BpuEI CTTGAG 1 cut(s) 90
BpuMI CCSGG 3 cut(s) 576, 704, 945
BsaHI GRCGYC 2 cut(s) 1029, 1851
BsaI GGTCTC 1 cut(s) 1487
BsaJI CCNNGG 5 cut(s) 9, 574, 1315, 2103, 2211
BsaXI ACNNNNNCTCC 8 cut(s) 1227, 1257, 1628, 1642, 1658, 1672, 2162, 2192
Bsc4I CCNNNNNNNGG 4 cut(s) 552, 639, 905, 1394
Bse118I RCCGGY 1 cut(s) 870
Bse1I ACTGG 3 cut(s) 749, 2049, 2266
BseBI CCWGG 4 cut(s) 117, 882, 984, 1075
BseDI CCNNGG 5 cut(s) 9, 574, 1315, 2103, 2211
BseGI GGATG 5 cut(s) 125, 478, 858, 1708, 2124
BseLI CCNNNNNNNGG 4 cut(s) 552, 639, 905, 1394
BseMII CTCAG 2 cut(s) 9, 2166
BseNI ACTGG 3 cut(s) 749, 2049, 2266
BseRI GAGGAG 3 cut(s) 365, 1636, 2183
BseSI GKGCMC 1 cut(s) 932
BseXI GCAGC 8 cut(s) 46, 52, 70, 239, 672, 751, 754, 2191
BsgI GTGCAG 2 cut(s) 679, 1024
Bsh1285I CGRYCG 2 cut(s) 832, 1972
BshFI GGCC 7 cut(s) 8, 326, 503, 880, 1203, 1918, 2083
BshNI GGYRCC 1 cut(s) 13
BsiEI CGRYCG 2 cut(s) 832, 1972
BsiHKAI GWGCWC 2 cut(s) 560, 932
BsiHKCI CYCGRG 2 cut(s) 333, 2186
BsiSI CCGG 7 cut(s) 546, 576, 704, 871, 944, 1919, 2160
BslFI GGGAC 4 cut(s) 557, 960, 1363, 1804
BslI CCNNNNNNNGG 4 cut(s) 552, 639, 905, 1394
BsmAI GTCTC 8 cut(s) 1153, 1162, 1241, 1487, 1668, 2015, 2035, 2165
BsmBI CGTCTC 1 cut(s) 1668
BsmFI GGGAC 4 cut(s) 557, 960, 1363, 1804
BsmI GAATGC 1 cut(s) 588
BsnI GGCC 7 cut(s) 8, 326, 503, 880, 1203, 1918, 2083
Bso31I GGTCTC 1 cut(s) 1487
BsoBI CYCGRG 2 cut(s) 333, 2186
Bsp1286I GDGCHC 2 cut(s) 560, 932
Bsp143I GATC 8 cut(s) 66, 139, 211, 1238, 1414, 1759, 1807, 1969
Bsp19I CCATGG 1 cut(s) 2103
BspACI CCGC 7 cut(s) 56, 135, 933, 1200, 1268, 1877, 2229
BspANI GGCC 7 cut(s) 8, 326, 503, 880, 1203, 1918, 2083
BspCNI CTCAG 2 cut(s) 10, 2167
BspLI GGNNCC 2 cut(s) 15, 1809
BspMAI CTGCAG 2 cut(s) 88, 324
BspPI GGATC 3 cut(s) 1246, 1802, 1815
BspT107I GGYRCC 1 cut(s) 13
BspTI CTTAAG 1 cut(s) 1277
BspTNI GGTCTC 1 cut(s) 1487
BsrFI RCCGGY 1 cut(s) 870
BsrI ACTGG 3 cut(s) 749, 2049, 2266
BssAI RCCGGY 1 cut(s) 870
BssECI CCNNGG 5 cut(s) 9, 574, 1315, 2103, 2211
BssMI GATC 8 cut(s) 66, 139, 211, 1238, 1414, 1759, 1807, 1969
BssNAI GTATAC 2 cut(s) 1043, 1516
BssNI GRCGYC 2 cut(s) 1029, 1851
BssSI CACGAG 1 cut(s) 1163
BssT1I CCWWGG 3 cut(s) 9, 1315, 2103
Bst1107I GTATAC 2 cut(s) 1043, 1516
Bst2BI CACGAG 1 cut(s) 1163
Bst2UI CCWGG 4 cut(s) 117, 882, 984, 1075
Bst4CI ACNGT 7 cut(s) 202, 257, 305, 571, 1339, 2026, 2240
BstACI GRCGYC 2 cut(s) 1029, 1851
BstAFI CTTAAG 1 cut(s) 1277
BstAPI GCANNNNNTGC 1 cut(s) 815
BstC8I GCNNGC 5 cut(s) 324, 878, 1224, 1942, 2276
BstDEI CTNAG 2 cut(s) 18, 2175
BstDSI CCRYGG 2 cut(s) 2103, 2211
BstF5I GGATG 5 cut(s) 125, 478, 858, 1708, 2124
BstH2I RGCGCY 2 cut(s) 101, 1401
BstHHI GCGC 3 cut(s) 100, 809, 1400
BstKTI GATC 8 cut(s) 69, 142, 214, 1241, 1417, 1762, 1810, 1972
BstMAI GTCTC 8 cut(s) 1153, 1162, 1241, 1487, 1668, 2015, 2035, 2165
BstMBI GATC 8 cut(s) 66, 139, 211, 1238, 1414, 1759, 1807, 1969
BstMCI CGRYCG 2 cut(s) 832, 1972
BstMWI GCNNNNNNNGC 4 cut(s) 555, 815, 2045, 2160
BstNI CCWGG 4 cut(s) 117, 882, 984, 1075
BstNSI RCATGY 1 cut(s) 2278
BstSCI CCNGG 7 cut(s) 115, 574, 702, 880, 943, 982, 1073
BstSFI CTRYAG 5 cut(s) 84, 222, 320, 383, 1855
BstSLI GKGCMC 1 cut(s) 932
BstV1I GCAGC 8 cut(s) 46, 52, 70, 239, 672, 751, 754, 2191
BstX2I RGATCY 2 cut(s) 1238, 1807
BstYI RGATCY 2 cut(s) 1238, 1807
BstZ17I GTATAC 2 cut(s) 1043, 1516
BsuI GTATCC 1 cut(s) 473
BsuRI GGCC 7 cut(s) 8, 326, 503, 880, 1203, 1918, 2083
BtgI CCRYGG 2 cut(s) 2103, 2211
BtrI CACGTC 1 cut(s) 1678
BtsCI GGATG 5 cut(s) 125, 478, 858, 1708, 2124
BtsI GCAGTG 1 cut(s) 1713
BtsIMutI CAGTG 2 cut(s) 756, 1713
Cac8I GCNNGC 5 cut(s) 324, 878, 1224, 1942, 2276
CaiI CAGNNNCTG 2 cut(s) 724, 755
CfoI GCGC 3 cut(s) 100, 809, 1400
Cfr10I RCCGGY 1 cut(s) 870
Cfr13I GGNCC 1 cut(s) 1202
CseI GACGC 5 cut(s) 807, 957, 1037, 1360, 1801
Csp6I GTAC 9 cut(s) 14, 258, 317, 837, 941, 1522, 1536, 1801, 2179
CviQI GTAC 9 cut(s) 14, 258, 317, 837, 941, 1522, 1536, 1801, 2179
DdeI CTNAG 2 cut(s) 18, 2175
DpnI GATC 8 cut(s) 68, 141, 213, 1240, 1416, 1761, 1809, 1971
DpnII GATC 8 cut(s) 66, 139, 211, 1238, 1414, 1759, 1807, 1969
DraI TTTAAA 1 cut(s) 1018
EaeI YGGCCR 1 cut(s) 878
EciI GGCGGA 1 cut(s) 2244
Eco130I CCWWGG 3 cut(s) 9, 1315, 2103
Eco147I AGGCCT 1 cut(s) 2083
Eco31I GGTCTC 1 cut(s) 1487
Eco47III AGCGCT 1 cut(s) 99
Eco57I CTGAAG 1 cut(s) 1171
Eco88I CYCGRG 2 cut(s) 333, 2186
EcoRI GAATTC 1 cut(s) 173
EcoRII CCWGG 4 cut(s) 115, 880, 982, 1073
EcoT14I CCWWGG 3 cut(s) 9, 1315, 2103
ErhI CCWWGG 3 cut(s) 9, 1315, 2103
Esp3I CGTCTC 1 cut(s) 1668
FalI AAGNNNNNCTT 2 cut(s) 1779, 1811
FaqI GGGAC 4 cut(s) 557, 960, 1363, 1804
FbaI TGATCA 1 cut(s) 1414
FblI GTMKAC 3 cut(s) 828, 1042, 1515
FokI GGATG 5 cut(s) 132, 485, 865, 1715, 2131
FspBI CTAG 8 cut(s) 80, 1025, 1227, 1757, 1781, 1874, 1914, 1937
FspI TGCGCA 1 cut(s) 808
GlaI GCGC 3 cut(s) 99, 808, 1399
GsuI CTGGAG 2 cut(s) 544, 966
HaeII RGCGCY 2 cut(s) 101, 1401
HaeIII GGCC 7 cut(s) 8, 326, 503, 880, 1203, 1918, 2083
HapII CCGG 7 cut(s) 546, 576, 704, 871, 944, 1919, 2160
HgaI GACGC 5 cut(s) 807, 957, 1037, 1360, 1801
HhaI GCGC 3 cut(s) 100, 809, 1400
Hin1I GRCGYC 2 cut(s) 1029, 1851
Hin6I GCGC 3 cut(s) 98, 807, 1398
HinP1I GCGC 3 cut(s) 98, 807, 1398
HincII GTYRAC 2 cut(s) 301, 829
HindII GTYRAC 2 cut(s) 301, 829
HindIII AAGCTT 1 cut(s) 1942
HinfI GANTC 9 cut(s) 89, 453, 707, 1161, 1170, 1978, 1984, 2156, 2198
HpaII CCGG 7 cut(s) 546, 576, 704, 871, 944, 1919, 2160
Hpy166II GTNNAC 9 cut(s) 93, 198, 301, 829, 930, 1043, 1150, 1342, 1516
Hpy188I TCNGA 5 cut(s) 1190, 1615, 1729, 1736, 1983
Hpy188III TCNNGA 8 cut(s) 373, 561, 1165, 1492, 1673, 1757, 1811, 1988
Hpy8I GTNNAC 9 cut(s) 93, 198, 301, 829, 930, 1043, 1150, 1342, 1516
Hpy99I CGWCG 2 cut(s) 1853, 2078
HpyAV CCTTC 8 cut(s) 271, 405, 458, 514, 679, 884, 1316, 2108
HpyCH4III ACNGT 7 cut(s) 202, 257, 305, 571, 1339, 2026, 2240
HpyCH4IV ACGT 5 cut(s) 444, 695, 1677, 1744, 1851
HpyF10VI GCNNNNNNNGC 4 cut(s) 555, 815, 2045, 2160
HpyF3I CTNAG 2 cut(s) 18, 2175
HpySE526I ACGT 5 cut(s) 444, 695, 1677, 1744, 1851
Hsp92I GRCGYC 2 cut(s) 1029, 1851
HspAI GCGC 3 cut(s) 98, 807, 1398
KpnI GGTACC 1 cut(s) 17
Ksp22I TGATCA 1 cut(s) 1414
Kzo9I GATC 8 cut(s) 66, 139, 211, 1238, 1414, 1759, 1807, 1969
LmnI GCTCC 5 cut(s) 548, 563, 1405, 1736, 1879
Lsp1109I GCAGC 8 cut(s) 46, 52, 70, 239, 672, 751, 754, 2191
LweI GCATC 6 cut(s) 15, 110, 174, 1558, 1619, 1981
MaeI CTAG 8 cut(s) 80, 1025, 1227, 1757, 1781, 1874, 1914, 1937
MaeII ACGT 5 cut(s) 444, 695, 1677, 1744, 1851
MaeIII GTNAC 7 cut(s) 202, 309, 440, 884, 2190, 2234, 2251
MalI GATC 8 cut(s) 68, 141, 213, 1240, 1416, 1761, 1809, 1971
MboI GATC 8 cut(s) 66, 139, 211, 1238, 1414, 1759, 1807, 1969
MboII GAAGA 7 cut(s) 34, 68, 418, 512, 800, 1615, 1947
MfeI CAATTG 1 cut(s) 1959
MflI RGATCY 2 cut(s) 1238, 1807
MhlI GDGCHC 2 cut(s) 560, 932
MlsI TGGCCA 1 cut(s) 880
MluNI TGGCCA 1 cut(s) 880
MlyI GAGTC 4 cut(s) 98, 462, 1155, 1164
MmeI TCCRAC 1 cut(s) 1427
Mox20I TGGCCA 1 cut(s) 880
MroXI GAANNNNTTC 2 cut(s) 496, 1818
MscI TGGCCA 1 cut(s) 880
MseI TTAA 7 cut(s) 624, 1017, 1115, 1124, 1278, 1425, 2291
MslI CAYNNNNRTG 2 cut(s) 1000, 1305
Msp20I TGGCCA 1 cut(s) 880
MspA1I CMGCKG 2 cut(s) 1834, 2204
MspCI CTTAAG 1 cut(s) 1277
MspI CCGG 7 cut(s) 546, 576, 704, 871, 944, 1919, 2160
MspR9I CCNGG 7 cut(s) 117, 576, 704, 882, 945, 984, 1075
MunI CAATTG 1 cut(s) 1959
Mva1269I GAATGC 1 cut(s) 588
MvaI CCWGG 4 cut(s) 117, 882, 984, 1075
MwoI GCNNNNNNNGC 4 cut(s) 555, 815, 2045, 2160
NciI CCSGG 3 cut(s) 576, 704, 945
NcoI CCATGG 1 cut(s) 2103
NdeII GATC 8 cut(s) 66, 139, 211, 1238, 1414, 1759, 1807, 1969
NlaIV GGNNCC 2 cut(s) 15, 1809
NmuCI GTSAC 2 cut(s) 2190, 2234
NsbI TGCGCA 1 cut(s) 808
NspI RCATGY 1 cut(s) 2278
PaeI GCATGC 1 cut(s) 2278
PceI AGGCCT 1 cut(s) 2083
PctI GAATGC 1 cut(s) 588
PdmI GAANNNNTTC 2 cut(s) 496, 1818
PfeI GAWTC 5 cut(s) 707, 1978, 1984, 2156, 2198
PflFI GACNNNGTC 1 cut(s) 535
PflMI CCANNNNNTGG 1 cut(s) 1394
PfoI TCCNGGA 2 cut(s) 982, 1073
Ple19I CGATCG 1 cut(s) 1972
PleI GAGTC 4 cut(s) 97, 461, 1155, 1164
PpsI GAGTC 4 cut(s) 97, 461, 1155, 1164
Psp1406I AACGTT 1 cut(s) 444
Psp6I CCWGG 4 cut(s) 115, 880, 982, 1073
PspGI CCWGG 4 cut(s) 115, 880, 982, 1073
PspN4I GGNNCC 2 cut(s) 15, 1809
PspPI GGNCC 1 cut(s) 1202
PstI CTGCAG 2 cut(s) 88, 324
PstNI CAGNNNCTG 2 cut(s) 724, 755
PsuI RGATCY 2 cut(s) 1238, 1807
PsyI GACNNNGTC 1 cut(s) 535
PvuI CGATCG 1 cut(s) 1972
PvuII CAGCTG 2 cut(s) 1834, 2204
RsaI GTAC 9 cut(s) 15, 259, 318, 838, 942, 1523, 1537, 1802, 2180
RsaNI GTAC 9 cut(s) 14, 258, 317, 837, 941, 1522, 1536, 1801, 2179
RseI CAYNNNNRTG 2 cut(s) 1000, 1305
SalI GTCGAC 1 cut(s) 827
SaqAI TTAA 7 cut(s) 624, 1017, 1115, 1124, 1278, 1425, 2291
Sau3AI GATC 8 cut(s) 66, 139, 211, 1238, 1414, 1759, 1807, 1969
Sau96I GGNCC 1 cut(s) 1202
ScaI AGTACT 2 cut(s) 318, 2180
SchI GAGTC 4 cut(s) 98, 462, 1155, 1164
ScrFI CCNGG 7 cut(s) 117, 576, 704, 882, 945, 984, 1075
SduI GDGCHC 2 cut(s) 560, 932
SfaNI GCATC 6 cut(s) 15, 110, 174, 1558, 1619, 1981
SfcI CTRYAG 5 cut(s) 84, 222, 320, 383, 1855
SmiMI CAYNNNNRTG 2 cut(s) 1000, 1305
SmlI CTYRAG 2 cut(s) 105, 1277
SmoI CTYRAG 2 cut(s) 105, 1277
SpeI ACTAGT 1 cut(s) 1780
SphI GCATGC 1 cut(s) 2278
SseBI AGGCCT 1 cut(s) 2083
SsiI CCGC 7 cut(s) 56, 135, 933, 1200, 1268, 1877, 2229
SspI AATATT 1 cut(s) 2221
SspMI CTAG 8 cut(s) 80, 1025, 1227, 1757, 1781, 1874, 1914, 1937
StuI AGGCCT 1 cut(s) 2083
StyD4I CCNGG 7 cut(s) 115, 574, 702, 880, 943, 982, 1073
StyI CCWWGG 3 cut(s) 9, 1315, 2103
TaaI ACNGT 7 cut(s) 202, 257, 305, 571, 1339, 2026, 2240
TaiI ACGT 5 cut(s) 447, 698, 1680, 1747, 1854
TaqI TCGA 8 cut(s) 182, 699, 828, 1491, 1545, 1672, 2166, 2246
TaqII GACCGA 3 cut(s) 846, 1511, 1853
TatI WGTACW 3 cut(s) 316, 1521, 2178
TauI GCSGC 2 cut(s) 59, 1203
TfiI GAWTC 5 cut(s) 707, 1978, 1984, 2156, 2198
Tru1I TTAA 7 cut(s) 624, 1017, 1115, 1124, 1278, 1425, 2291
Tru9I TTAA 7 cut(s) 624, 1017, 1115, 1124, 1278, 1425, 2291
TscAI CASTG 2 cut(s) 756, 1720
TseFI GTSAC 2 cut(s) 2190, 2234
TseI GCWGC 8 cut(s) 40, 59, 83, 227, 660, 739, 742, 2204
Tsp45I GTSAC 2 cut(s) 2190, 2234
TspGWI ACGGA 2 cut(s) 699, 2200
TspRI CASTG 2 cut(s) 756, 1720
Tth111I GACNNNGTC 1 cut(s) 535
Van91I CCANNNNNTGG 1 cut(s) 1394
Vha464I CTTAAG 1 cut(s) 1277
VneI GTGCAC 1 cut(s) 928
XapI RAATTY 6 cut(s) 173, 496, 688, 973, 1080, 2280
XbaI TCTAGA 1 cut(s) 1756
XceI RCATGY 1 cut(s) 2278
XmiI GTMKAC 3 cut(s) 828, 1042, 1515
XmnI GAANNNNTTC 2 cut(s) 496, 1818
XspI CTAG 8 cut(s) 80, 1025, 1227, 1757, 1781, 1874, 1914, 1937
ZraI GACGTC 1 cut(s) 1852
ZrmI AGTACT 2 cut(s) 318, 2180
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.