RchiOBHm_Chr5g0017091

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000066
Physical Location & Seq
Reverse (-)
11767515 .. 11770094
2580 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29741

Sequence Viewer

Length: 1545 bp
ATGGACGTCAGCCAACCAACCGATCCCCCGATAGCATATTTGACCAAAAACGGTACTGTCATCATTACCAACATATCCCTTGCTGAGGGTGAGTTGCAAATAACAGACTTTATAGCTCGAGATTGCTATAGCGCCTCTGCTCTAAGAACCAGAAGGAACAGCCCTGTGCTACGGCTGCCTTCTCCTTACACCATCTGTGATACCAAAAACAACTTCATTGCCGTTGGTTGTGACACTCACGCAATTTTCAAAGGATTCCGGGGCACAGAACAGTCCATAACTGGGTGCATGTCAGTATGTAACAGCCAAGAAAGCGTTGATCAGAATTCTTGCTCCGGGGTTGGGTGTTGCCAAACAAACATTCCTAGCGGACTGCATAACCGTACTGTGACACTGGATAGCTATAGCAATCATTCAGGTATATGGGACTTCAACCCCTGCAGCTTTGCATTCATTGTTGAAGAGACACTGTTCAAGTTCTCCGGGAACACAAGTTTTCAACAACTGAACAGCACTACACGGCTTCCAATGGTTCTTAATTGGGCAATTGGGGATGAGCCAGACCCATGTGATGTAGCTGAAAAGAGGCAGGATTTAAATATTGATGAGTGCAAGGATCCAAACCTCTGCAACCATGGAGAGTGCAAAAATTTTGCGGGAAGTTACTCTTGTTTATGCTCAAAAGGATACAAGATCGACAGCATGAATGACAAGAGTTGCATCAAAGAGAATCCCAAAAATAAATCAAAGATGACTCTCCTGCTTATTATTTCACTGAGTGTAAGTATAGGCTTATTGGTTTTATTTGTGGGGATTTCGTGGATATGTTTAGGGATAAAGAGAAGACAATACACTAAACTCAAAGAAAAGTACTTCAAAGAGAATGGTGGCTTATTGTTACTGCAACAACTTGCTAGTCATGCAGGCACTGTGGATACAACAAAAATCTTTTCCACAGAAGAACTTGAGAAGGCAACAAACAGTTACCACGAGAGTAGAATCCTTGGAGAAGGAGGCTATGGAACAGTTTACAAAGGAATACTACCAGATGACAAAGTGGTGGCCATAAAGAAGTCAAAAGGTGGTGCCCCAACCCAAAGTGATCAGTTTGTTAACGAGGTGATTGTTCTTTCTCAAATAAACCACAGAAATGTGGTGAAGCTACTAGGTTGTTGTTTAGAGACAGAAGTGCCTTTACTAGTATATGAGTTCATCACCCATGGAACTCTTTATGAGCATATTCATTATAAAAGATCATCATTGTCATTCGAATTACGAATGAAGATAGCAGTTGAAACAGCAGAAGCACTAGCATACTTACACTCCTCAACTTCAAATCCAATCATACATCGAGATGTGAAAGCAGAAAATATACTCTTAGATGATGCTTATACAGCAAAAGTGTCAGACTTTGGAGCTTCACGATTGGTTCCATCAGGTCAAACTGAAATACAAACTCTAGTGCTAGGGACATTCGGTTACCTAGACCCTGAATATCTGCAATCGAACCAGGTAACCCTGAATATCTGCAATCGAACCAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK

Protein Analysis

514

Amino Acids

56.87

Weight (kDa)

6.09

Isoelectric Point (pI)

38.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EGF_CA PF07645 201 - 240 3e-07 Calcium-binding EGF domain
Pkinase PF00069 329 - 506 7.7e-37 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 332 - 506 1.1e-35 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21210 AT1G21230 AT1G21240 AT1G21240 AT1G21245 AT1G21250 AT1G21270 AT1G22720 AT3G25490
fragaria_vesca FvH4_2g35051 FvH4_2g35051 FvH4_2g35060 FvH4_3g10281 FvH4_3g10285 FvH4_3g10286 FvH4_3g10311 FvH4_3g10311 FvH4_3g10320 FvH4_3g10332 FvH4_3g10340 FvH4_3g10350 FvH4_3g10360 FvH4_3g10390 FvH4_3g10412 FvH4_3g10413 FvH4_3g10461 FvH4_3g10462 FvH4_3g10470 FvH4_3g10510 FvH4_3g10520 FvH4_3g10530 FvH4_3g10550 FvH4_3g10560 FvH4_3g10611 FvH4_3g11730 FvH4_6g53140 FvH4_6g53142 FvH4_7g31880 FvH4_7g31910 FvH4_7g31920
malus_domestica MD05G1269900.v1.1 MD05G1270000.v1.1 MD05G1270200.v1.1 MD05G1270400.v1.1 MD05G1270500.v1.1 MD05G1270600.v1.1 MD05G1270800.v1.1 MD05G1270900.v1.1 MD07G1183800.v1.1 MD08G1107600.v1.1 MD10G1248500.v1.1 MD10G1249200.v1.1 MD10G1249500.v1.1 MD10G1250000.v1.1 MD10G1250500.v1.1 MD10G1250900.v1.1 MD10G1251200.v1.1 MD10G1251400.v1.1 MD13G1004600.v1.1 MD15G1088400.v1.1
prunus_persica Prupe.1G442400_v2.0.a1 Prupe.1G442500_v2.0.a1 Prupe.2G223400_v2.0.a1 Prupe.4G091900_v2.0.a1 Prupe.4G093200_v2.0.a1 Prupe.4G093300_v2.0.a1 Prupe.4G093400_v2.0.a1 Prupe.4G093500_v2.0.a1 Prupe.4G094100_v2.0.a1 Prupe.4G094200_v2.0.a1 Prupe.4G094300_v2.0.a1 Prupe.6G358300_v2.0.a1
pyrus_communis pycom08g08990 pycom10g20660 pycom10g20670 pycom10g20680 pycom10g20710 pycom10g20740 pycom13g00400 pycom15g08290
rosa_chinensis RchiOBHm_Chr1g0326861 RchiOBHm_Chr1g0326871 RchiOBHm_Chr1g0331391 RchiOBHm_Chr1g0336911 RchiOBHm_Chr1g0364001 RchiOBHm_Chr2g0174541 RchiOBHm_Chr2g0175041 RchiOBHm_Chr2g0175101 RchiOBHm_Chr2g0175121 RchiOBHm_Chr3g0486331 RchiOBHm_Chr5g0015581 RchiOBHm_Chr5g0016551 RchiOBHm_Chr5g0016561 RchiOBHm_Chr5g0016581 RchiOBHm_Chr5g0016601 RchiOBHm_Chr5g0016611 RchiOBHm_Chr5g0016641 RchiOBHm_Chr5g0016661 RchiOBHm_Chr5g0016691 RchiOBHm_Chr5g0016701 RchiOBHm_Chr5g0016731 RchiOBHm_Chr5g0016741 RchiOBHm_Chr5g0016811 RchiOBHm_Chr5g0016841 RchiOBHm_Chr5g0016871 RchiOBHm_Chr5g0016881 RchiOBHm_Chr5g0016931 RchiOBHm_Chr5g0017021 RchiOBHm_Chr5g0017041 RchiOBHm_Chr5g0017071 RchiOBHm_Chr5g0017091 RchiOBHm_Chr5g0017121 RchiOBHm_Chr5g0017141 RchiOBHm_Chr5g0017291 RchiOBHm_Chr5g0017321 RchiOBHm_Chr5g0017401 RchiOBHm_Chr5g0019001 RchiOBHm_Chr5g0019071 RchiOBHm_Chr5g0028941 RchiOBHm_Chr5g0052141 RchiOBHm_Chr5g0054721 RchiOBHm_Chr5g0054801 RchiOBHm_Chr5g0054931 RchiOBHm_Chr5g0054941 RchiOBHm_Chr6g0259291 RchiOBHm_Chr6g0292991 RchiOBHm_Chr6g0306911
rosa_laevigata RLG00000009465 RLG00000009468 RLG00000010755 RLG00000011976 RLG00000014541 RLG00000014542 RLG00000015169 RLG00000022308 RLG00000027529 RLG00000029324 RLG00000030244 RLG00000030245 RLG00000032210 RLG00000032211 RLG00000032257 RLG00000032259 RLG00000032260 RLG00000032261 RLG00000032262 RLG00000032268 RLG00000032269 RLG00000032272 RLG00000032282 RLG00000032283 RLG00000032308 RLG00000032413 RLG00000033129 RLG00000034945
rosa_multiflora Rmu_co8241929.1_g000001 Rmu_co8269227.1_g000001 Rmu_co8461259.1_g000001 Rmu_co8495385.1_g000001 Rmu_sc0000028.1_g000003 Rmu_sc0000083.1_g000009 Rmu_sc0000083.1_g000010 Rmu_sc0000083.1_g000031 Rmu_sc0000083.1_g000043 Rmu_sc0000083.1_g000046 Rmu_sc0000083.1_g000047 Rmu_sc0000563.1_g000024 Rmu_sc0000842.1_g000012 Rmu_sc0001235.1_g000001 Rmu_sc0001235.1_g000005 Rmu_sc0001235.1_g000009 Rmu_sc0001235.1_g000015 Rmu_sc0001235.1_g000016 Rmu_sc0001235.1_g000017 Rmu_sc0001473.1_g000036 Rmu_sc0001607.1_g000016 Rmu_sc0001788.1_g000006 Rmu_sc0002018.1_g000008 Rmu_sc0002028.1_g000001 Rmu_sc0002680.1_g000011 Rmu_sc0002798.1_g000023 Rmu_sc0002886.1_g000023 Rmu_sc0002989.1_g000012 Rmu_sc0003064.1_g000009 Rmu_sc0003382.1_g000006 Rmu_sc0003630.1_g000002 Rmu_sc0003630.1_g000047 Rmu_sc0003630.1_g000066 Rmu_sc0003630.1_g000067 Rmu_sc0003649.1_g000010 Rmu_sc0003688.1_g000005 Rmu_sc0003920.1_g000007 Rmu_sc0004765.1_g000007 Rmu_sc0004765.1_g000008 Rmu_sc0004920.1_g000011 Rmu_sc0005223.1_g000008 Rmu_sc0005223.1_g000010 Rmu_sc0005223.1_g000015 Rmu_sc0005223.1_g000016 Rmu_sc0005319.1_g000014 Rmu_sc0005319.1_g000015 Rmu_sc0005319.1_g000018 Rmu_sc0005319.1_g000019 Rmu_sc0005319.1_g000025 Rmu_sc0005418.1_g000007 Rmu_sc0005418.1_g000012 Rmu_sc0006106.1_g000011 Rmu_sc0006914.1_g000012 Rmu_sc0007023.1_g000001 Rmu_sc0007662.1_g000001 Rmu_sc0007920.1_g000002 Rmu_sc0007920.1_g000013 Rmu_sc0008053.1_g000016 Rmu_sc0008053.1_g000017 Rmu_sc0008053.1_g000018 Rmu_sc0008053.1_g000023 Rmu_sc0008053.1_g000050 Rmu_sc0009042.1_g000007 Rmu_sc0009042.1_g000008 Rmu_sc0010642.1_g000008 Rmu_sc0011109.1_g000002 Rmu_sc0011314.1_g000006 Rmu_sc0011974.1_g000006 Rmu_sc0012179.1_g000009 Rmu_sc0015169.1_g000001 Rmu_sc0016368.1_g000002 Rmu_sc0022659.1_g000001 Rmu_sc0027371.1_g000001 Rmu_sc0028213.1_g000001 Rmu_sc0034526.1_g000001 Rmu_sc0039366.1_g000002 Rmu_ssc0000050.1_g000084 Rmu_ssc0000050.1_g000088 Rmu_ssc0000167.1_g000017
rosa_roxburghii Rroxscaffold_1G00011340 Rroxscaffold_1G00025710 Rroxscaffold_1G00025870 Rroxscaffold_1G00026070 Rroxscaffold_1G00026170 Rroxscaffold_1G00026200 Rroxscaffold_1G00026270 Rroxscaffold_1G00050950 Rroxscaffold_1G00058900 Rroxscaffold_1G00060040 Rroxscaffold_1G00060060 Rroxscaffold_1G00060070 Rroxscaffold_1G00060080 Rroxscaffold_1G00060110 Rroxscaffold_1G00060120 Rroxscaffold_1G00060140 Rroxscaffold_1G00060170 Rroxscaffold_1G00060210 Rroxscaffold_1G00060220 Rroxscaffold_1G00060240 Rroxscaffold_1G00060250 Rroxscaffold_1G00060260 Rroxscaffold_1G00060300 Rroxscaffold_1G00060320 Rroxscaffold_1G00060330 Rroxscaffold_1G00060900 Rroxscaffold_1G00060920 Rroxscaffold_1G00073980 Rroxscaffold_2G00077560 Rroxscaffold_2G00077580 Rroxscaffold_2G00146990 Rroxscaffold_3G00236200 Rroxscaffold_3G00236210 Rroxscaffold_3G00236220 Rroxscaffold_4G00292760 Rroxscaffold_4G00315200 Rroxscaffold_4G00315240 Rroxscaffold_4G00326240 Rroxscaffold_4G00328980 Rroxscaffold_5G00341890 Rroxscaffold_7G00161350 Rroxscaffold_7G00174490 Rroxscaffold_7G00206550
rosa_rugosa Rorug01G0093000 Rorug01G0093100 Rorug01G0128800 Rorug01G0312000 Rorug01G0312000 Rorug02G0581100 Rorug03G0224600 Rorug05G0028500 Rorug05G0032700 Rorug05G0032800 Rorug05G0032900 Rorug05G0032900 Rorug05G0033000 Rorug05G0033100 Rorug05G0033300 Rorug05G0033400 Rorug05G0033900 Rorug05G0034900 Rorug05G0035000 Rorug05G0046900 Rorug05G0047500 Rorug05G0047500 Rorug05G0111600 Rorug05G0287900 Rorug05G0288700 Rorug05G0586900 Rorug06G0231700 Rorug06G0354300 Rorug06G0354400
rosa_samantha Rh1DG122200 Rh2DG686800 Rh5BG136600 Rh6AG344500 Rh6BG351900 Rh6CG359000 Rh6CG359100 Rh6CG387600 Rh6CG480500 Rh6DG344600 Rh6DG467400
rosa_wichuraiana Rw0G010250 Rw0G014820 Rw0G021400 Rw0G023100 Rw1G009450 Rw1G012100 Rw1G012180 Rw1G028360 Rw2G054260 Rw3G024370 Rw3G024420 Rw3G024470 Rw5G010850 Rw5G011010 Rw5G011020 Rw5G011050 Rw5G011080 Rw5G011120 Rw5G012300 Rw5G018470 Rw5G033830 Rw6G008930 Rw6G030070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1248
AatII GACGTC 1 cut(s) 9
AccB1I GGYRCC 1 cut(s) 1085
AciI CCGC 2 cut(s) 369, 656
AclWI GGATC 3 cut(s) 17, 611, 624
AcoI YGGCCR 1 cut(s) 1062
AcsI RAATTY 2 cut(s) 325, 649
AcyI GRCGYC 1 cut(s) 6
AdeI CACNNNGTG 1 cut(s) 779
AfaI GTAC 3 cut(s) 55, 385, 872
AfiI CCNNNNNNNGG 3 cut(s) 85, 282, 342
AgsI TTSAA 8 cut(s) 250, 433, 461, 475, 500, 877, 1295, 1335
AhlI ACTAGT 1 cut(s) 1198
AjnI CCWGG 1 cut(s) 1509
AjuI GAANNNNNNNTTGG 4 cut(s) 197, 229, 345, 377
AluBI AGCT 6 cut(s) 116, 402, 444, 578, 1162, 1418
AluI AGCT 6 cut(s) 116, 402, 444, 578, 1162, 1418
Alw26I GTCTC 2 cut(s) 458, 1175
AlwI GGATC 3 cut(s) 17, 611, 624
AlwNI CAGNNNCTG 1 cut(s) 929
Ama87I CYCGRG 1 cut(s) 117
AoxI GGCC 1 cut(s) 1062
ApeKI GCWGC 2 cut(s) 175, 441
ApoI RAATTY 2 cut(s) 325, 649
AspLEI GCGC 1 cut(s) 134
AsuC2I CCSGG 3 cut(s) 260, 337, 484
AsuHPI GGTGA 4 cut(s) 101, 1132, 1168, 1207
AsuII TTCGAA 1 cut(s) 1269
AvaI CYCGRG 1 cut(s) 117
BaeGI GKGCMC 2 cut(s) 266, 1090
BalI TGGCCA 1 cut(s) 1064
BamHI GGATCC 1 cut(s) 616
BanI GGYRCC 1 cut(s) 1085
BarI GAAGNNNNNNTAC 2 cut(s) 1179, 1211
BauI CACGAG 1 cut(s) 989
BbsI GAAGAC 1 cut(s) 850
BbvCI CCTCAGC 1 cut(s) 84
BbvI GCAGC 2 cut(s) 162, 453
BccI CCATC 2 cut(s) 200, 1441
BceAI ACGGC 3 cut(s) 188, 206, 536
BciT130I CCWGG 1 cut(s) 1511
BciVI GTATCC 2 cut(s) 680, 928
BclI TGATCA 2 cut(s) 319, 1102
BcnI CCSGG 3 cut(s) 260, 337, 484
BcoDI GTCTC 2 cut(s) 458, 1175
BcuI ACTAGT 1 cut(s) 1198
BfaI CTAG 8 cut(s) 366, 915, 1166, 1199, 1310, 1460, 1466, 1484
BfmI CTRYAG 3 cut(s) 127, 403, 439
BfoI RGCGCY 1 cut(s) 135
BfuI GTATCC 2 cut(s) 680, 928
BisI GCNGC 2 cut(s) 176, 442
BlsI GCNGC 2 cut(s) 177, 443
BmcAI AGTACT 1 cut(s) 872
Bme1390I CCNGG 4 cut(s) 260, 337, 484, 1511
BmeT110I CYCGRG 1 cut(s) 117
BmiI GGNNCC 3 cut(s) 618, 1087, 1431
BmrFI CCNGG 4 cut(s) 260, 337, 484, 1511
BmrI ACTGGG 1 cut(s) 291
BmsI GCATC 2 cut(s) 729, 1375
BmuI ACTGGG 1 cut(s) 291
BpiI GAAGAC 1 cut(s) 850
Bpu10I CCTNAGC 1 cut(s) 84
Bpu14I TTCGAA 1 cut(s) 1269
BpuEI CTTGAG 1 cut(s) 986
BpuMI CCSGG 3 cut(s) 260, 337, 484
BsaHI GRCGYC 1 cut(s) 6
BsaJI CCNNGG 5 cut(s) 259, 336, 634, 1003, 1219
BsaXI ACNNNNNCTCC 2 cut(s) 1307, 1337
Bsc4I CCNNNNNNNGG 3 cut(s) 85, 282, 342
Bse1I ACTGG 2 cut(s) 286, 399
Bse3DI GCAATG 1 cut(s) 216
BseBI CCWGG 1 cut(s) 1511
BseDI CCNNGG 5 cut(s) 259, 336, 634, 1003, 1219
BseGI GGATG 1 cut(s) 559
BseLI CCNNNNNNNGG 3 cut(s) 85, 282, 342
BseMI GCAATG 1 cut(s) 216
BseMII CTCAG 2 cut(s) 75, 767
BseNI ACTGG 2 cut(s) 286, 399
BseRI GAGGAG 1 cut(s) 1315
BseSI GKGCMC 2 cut(s) 266, 1090
BseXI GCAGC 2 cut(s) 162, 453
BshFI GGCC 1 cut(s) 1064
BshNI GGYRCC 1 cut(s) 1085
BsiHKCI CYCGRG 1 cut(s) 117
BsiSI CCGG 3 cut(s) 259, 336, 483
BslFI GGGAC 2 cut(s) 440, 1483
BslI CCNNNNNNNGG 3 cut(s) 85, 282, 342
BsmAI GTCTC 2 cut(s) 458, 1175
BsmFI GGGAC 2 cut(s) 440, 1483
BsmI GAATGC 1 cut(s) 449
BsnI GGCC 1 cut(s) 1064
BsoBI CYCGRG 1 cut(s) 117
Bsp119I TTCGAA 1 cut(s) 1269
Bsp1286I GDGCHC 2 cut(s) 266, 1090
Bsp143I GATC 6 cut(s) 22, 319, 616, 693, 1102, 1253
Bsp19I CCATGG 2 cut(s) 634, 1219
BspACI CCGC 2 cut(s) 369, 656
BspANI GGCC 1 cut(s) 1064
BspCNI CTCAG 2 cut(s) 76, 768
BspLI GGNNCC 3 cut(s) 618, 1087, 1431
BspMAI CTGCAG 1 cut(s) 443
BspPI GGATC 3 cut(s) 17, 611, 624
BspT104I TTCGAA 1 cut(s) 1269
BspT107I GGYRCC 1 cut(s) 1085
BsrDI GCAATG 1 cut(s) 216
BsrI ACTGG 2 cut(s) 286, 399
BssECI CCNNGG 5 cut(s) 259, 336, 634, 1003, 1219
BssMI GATC 6 cut(s) 22, 319, 616, 693, 1102, 1253
BssNI GRCGYC 1 cut(s) 6
BssSI CACGAG 1 cut(s) 989
BssT1I CCWWGG 3 cut(s) 634, 1003, 1219
Bst2BI CACGAG 1 cut(s) 989
Bst2UI CCWGG 1 cut(s) 1511
Bst4CI ACNGT 9 cut(s) 53, 58, 273, 383, 388, 471, 931, 983, 1027
Bst6I CTCTTC 1 cut(s) 456
BstACI GRCGYC 1 cut(s) 6
BstBI TTCGAA 1 cut(s) 1269
BstC8I GCNNGC 1 cut(s) 925
BstDEI CTNAG 4 cut(s) 84, 143, 776, 1378
BstDSI CCRYGG 2 cut(s) 634, 1219
BstEII GGTNACC 2 cut(s) 1478, 1513
BstENI CCTNNNNNAGG 1 cut(s) 83
BstF5I GGATG 1 cut(s) 559
BstH2I RGCGCY 1 cut(s) 135
BstHHI GCGC 1 cut(s) 134
BstKTI GATC 6 cut(s) 25, 322, 619, 696, 1105, 1256
BstMAI GTCTC 2 cut(s) 458, 1175
BstMBI GATC 6 cut(s) 22, 319, 616, 693, 1102, 1253
BstMWI GCNNNNNNNGC 4 cut(s) 175, 312, 920, 1394
BstNI CCWGG 1 cut(s) 1511
BstNSI RCATGY 1 cut(s) 292
BstPI GGTNACC 2 cut(s) 1478, 1513
BstSCI CCNGG 4 cut(s) 258, 335, 482, 1509
BstSFI CTRYAG 3 cut(s) 127, 403, 439
BstSLI GKGCMC 2 cut(s) 266, 1090
BstV1I GCAGC 2 cut(s) 162, 453
BstV2I GAAGAC 1 cut(s) 850
BstX2I RGATCY 1 cut(s) 616
BstYI RGATCY 1 cut(s) 616
BsuI GTATCC 2 cut(s) 680, 928
BsuRI GGCC 1 cut(s) 1064
BtgI CCRYGG 2 cut(s) 634, 1219
BtsCI GGATG 1 cut(s) 559
BtsIMutI CAGTG 4 cut(s) 392, 467, 773, 927
Cac8I GCNNGC 1 cut(s) 925
CaiI CAGNNNCTG 1 cut(s) 929
CfoI GCGC 1 cut(s) 134
CsiI ACCWGGT 1 cut(s) 1509
Csp6I GTAC 3 cut(s) 54, 384, 871
CviAII CATG 6 cut(s) 289, 567, 635, 703, 920, 1220
CviQI GTAC 3 cut(s) 54, 384, 871
DdeI CTNAG 4 cut(s) 84, 143, 776, 1378
DpnI GATC 6 cut(s) 24, 321, 618, 695, 1104, 1255
DpnII GATC 6 cut(s) 22, 319, 616, 693, 1102, 1253
DraI TTTAAA 1 cut(s) 597
DraIII CACNNNGTG 1 cut(s) 779
EaeI YGGCCR 1 cut(s) 1062
Eam1104I CTCTTC 1 cut(s) 456
EarI CTCTTC 1 cut(s) 456
Eco130I CCWWGG 3 cut(s) 634, 1003, 1219
Eco88I CYCGRG 1 cut(s) 117
Eco91I GGTNACC 2 cut(s) 1478, 1513
EcoNI CCTNNNNNAGG 1 cut(s) 83
EcoO65I GGTNACC 2 cut(s) 1478, 1513
EcoRI GAATTC 1 cut(s) 325
EcoRII CCWGG 1 cut(s) 1509
EcoT14I CCWWGG 3 cut(s) 634, 1003, 1219
ErhI CCWWGG 3 cut(s) 634, 1003, 1219
FaeI CATG 6 cut(s) 292, 570, 638, 706, 923, 1223
FalI AAGNNNNNCTT 2 cut(s) 652, 684
FaqI GGGAC 2 cut(s) 440, 1483
FatI CATG 6 cut(s) 288, 566, 634, 702, 919, 1219
FauI CCCGC 1 cut(s) 649
FbaI TGATCA 2 cut(s) 319, 1102
Fnu4HI GCNGC 2 cut(s) 176, 442
FokI GGATG 1 cut(s) 566
Fsp4HI GCNGC 2 cut(s) 176, 442
FspBI CTAG 8 cut(s) 366, 915, 1166, 1199, 1310, 1460, 1466, 1484
GlaI GCGC 1 cut(s) 133
GluI GCNGC 2 cut(s) 176, 442
HaeII RGCGCY 1 cut(s) 135
HaeIII GGCC 1 cut(s) 1064
HapII CCGG 3 cut(s) 259, 336, 483
HhaI GCGC 1 cut(s) 134
Hin1I GRCGYC 1 cut(s) 6
Hin1II CATG 6 cut(s) 292, 570, 638, 706, 923, 1223
Hin6I GCGC 1 cut(s) 132
HinP1I GCGC 1 cut(s) 132
HincII GTYRAC 1 cut(s) 1114
HindII GTYRAC 1 cut(s) 1114
HinfI GANTC 4 cut(s) 255, 730, 754, 999
HpaI GTTAAC 1 cut(s) 1114
HpaII CCGG 3 cut(s) 259, 336, 483
HphI GGTGA 4 cut(s) 101, 1132, 1168, 1207
Hpy166II GTNNAC 2 cut(s) 1030, 1114
Hpy188I TCNGA 2 cut(s) 324, 1408
Hpy188III TCNNGA 3 cut(s) 119, 1352, 1422
Hpy8I GTNNAC 2 cut(s) 1030, 1114
HpyAV CCTTC 4 cut(s) 147, 189, 964, 1004
HpyCH4III ACNGT 9 cut(s) 53, 58, 273, 383, 388, 471, 931, 983, 1027
HpyCH4IV ACGT 1 cut(s) 6
HpyF10VI GCNNNNNNNGC 4 cut(s) 175, 312, 920, 1394
HpyF3I CTNAG 4 cut(s) 84, 143, 776, 1378
HpySE526I ACGT 1 cut(s) 6
Hsp92I GRCGYC 1 cut(s) 6
Hsp92II CATG 6 cut(s) 292, 570, 638, 706, 923, 1223
HspAI GCGC 1 cut(s) 132
Ksp22I TGATCA 2 cut(s) 319, 1102
KspAI GTTAAC 1 cut(s) 1114
Kzo9I GATC 6 cut(s) 22, 319, 616, 693, 1102, 1253
LmnI GCTCC 2 cut(s) 338, 1415
Lsp1109I GCAGC 2 cut(s) 162, 453
LweI GCATC 2 cut(s) 729, 1375
MabI ACCWGGT 1 cut(s) 1509
MaeI CTAG 8 cut(s) 366, 915, 1166, 1199, 1310, 1460, 1466, 1484
MaeII ACGT 1 cut(s) 6
MaeIII GTNAC 8 cut(s) 230, 299, 388, 662, 897, 983, 1478, 1513
MalI GATC 6 cut(s) 24, 321, 618, 695, 1104, 1255
MboI GATC 6 cut(s) 22, 319, 616, 693, 1102, 1253
MboII GAAGA 4 cut(s) 473, 855, 971, 1294
MfeI CAATTG 1 cut(s) 546
MflI RGATCY 1 cut(s) 616
MhlI GDGCHC 2 cut(s) 266, 1090
MlsI TGGCCA 1 cut(s) 1064
MluCI AATT 6 cut(s) 243, 325, 538, 546, 649, 1271
MluNI TGGCCA 1 cut(s) 1064
MlyI GAGTC 1 cut(s) 748
MnlI CCTC 7 cut(s) 79, 145, 579, 635, 1007, 1111, 1336
Mox20I TGGCCA 1 cut(s) 1064
MscI TGGCCA 1 cut(s) 1064
MseI TTAA 3 cut(s) 537, 596, 1113
MslI CAYNNNNRTG 2 cut(s) 1149, 1353
Msp20I TGGCCA 1 cut(s) 1064
MspI CCGG 3 cut(s) 259, 336, 483
MspR9I CCNGG 4 cut(s) 260, 337, 484, 1511
MunI CAATTG 1 cut(s) 546
Mva1269I GAATGC 1 cut(s) 449
MvaI CCWGG 1 cut(s) 1511
MwoI GCNNNNNNNGC 4 cut(s) 175, 312, 920, 1394
NciI CCSGG 3 cut(s) 260, 337, 484
NcoI CCATGG 2 cut(s) 634, 1219
NdeII GATC 6 cut(s) 22, 319, 616, 693, 1102, 1253
NlaIII CATG 6 cut(s) 292, 570, 638, 706, 923, 1223
NlaIV GGNNCC 3 cut(s) 618, 1087, 1431
NmuCI GTSAC 2 cut(s) 230, 388
NspI RCATGY 1 cut(s) 292
NspV TTCGAA 1 cut(s) 1269
PaeR7I CTCGAG 1 cut(s) 117
PctI GAATGC 1 cut(s) 449
PfeI GAWTC 3 cut(s) 255, 730, 999
PfoI TCCNGGA 1 cut(s) 482
PkrI GCNGC 2 cut(s) 177, 443
PleI GAGTC 1 cut(s) 748
PpsI GAGTC 1 cut(s) 748
PsiI TTATAA 1 cut(s) 1248
Psp6I CCWGG 1 cut(s) 1509
PspEI GGTNACC 2 cut(s) 1478, 1513
PspGI CCWGG 1 cut(s) 1509
PspN4I GGNNCC 3 cut(s) 618, 1087, 1431
PstI CTGCAG 1 cut(s) 443
PstNI CAGNNNCTG 1 cut(s) 929
PsuI RGATCY 1 cut(s) 616
RsaI GTAC 3 cut(s) 55, 385, 872
RsaNI GTAC 3 cut(s) 54, 384, 871
RseI CAYNNNNRTG 2 cut(s) 1149, 1353
SaqAI TTAA 3 cut(s) 537, 596, 1113
SatI GCNGC 2 cut(s) 176, 442
Sau3AI GATC 6 cut(s) 22, 319, 616, 693, 1102, 1253
ScaI AGTACT 1 cut(s) 872
SchI GAGTC 1 cut(s) 748
ScrFI CCNGG 4 cut(s) 260, 337, 484, 1511
SduI GDGCHC 2 cut(s) 266, 1090
SexAI ACCWGGT 1 cut(s) 1509
SfaNI GCATC 2 cut(s) 729, 1375
SfcI CTRYAG 3 cut(s) 127, 403, 439
Sfr274I CTCGAG 1 cut(s) 117
SfuI TTCGAA 1 cut(s) 1269
SlaI CTCGAG 1 cut(s) 117
SmiI ATTTAAAT 1 cut(s) 597
SmiMI CAYNNNNRTG 2 cut(s) 1149, 1353
SmlI CTYRAG 2 cut(s) 117, 965
SmoI CTYRAG 2 cut(s) 117, 965
SpeI ACTAGT 1 cut(s) 1198
Sse9I AATT 6 cut(s) 243, 325, 538, 546, 649, 1271
SsiI CCGC 2 cut(s) 369, 656
SspI AATATT 1 cut(s) 601
SspMI CTAG 8 cut(s) 366, 915, 1166, 1199, 1310, 1460, 1466, 1484
StyD4I CCNGG 4 cut(s) 258, 335, 482, 1509
StyI CCWWGG 3 cut(s) 634, 1003, 1219
SwaI ATTTAAAT 1 cut(s) 597
TaaI ACNGT 9 cut(s) 53, 58, 273, 383, 388, 471, 931, 983, 1027
TaiI ACGT 1 cut(s) 9
TaqI TCGA 6 cut(s) 118, 696, 1269, 1351, 1505, 1534
TasI AATT 6 cut(s) 243, 325, 538, 546, 649, 1271
TatI WGTACW 1 cut(s) 870
TfiI GAWTC 3 cut(s) 255, 730, 999
Tru1I TTAA 3 cut(s) 537, 596, 1113
Tru9I TTAA 3 cut(s) 537, 596, 1113
TscAI CASTG 4 cut(s) 399, 474, 780, 934
TseFI GTSAC 2 cut(s) 230, 388
TseI GCWGC 2 cut(s) 175, 441
Tsp45I GTSAC 2 cut(s) 230, 388
TspDTI ATGAA 6 cut(s) 205, 442, 719, 1201, 1232, 1295
TspRI CASTG 4 cut(s) 399, 474, 780, 934
XagI CCTNNNNNAGG 1 cut(s) 83
XapI RAATTY 2 cut(s) 325, 649
XceI RCATGY 1 cut(s) 292
XhoI CTCGAG 1 cut(s) 117
XspI CTAG 8 cut(s) 366, 915, 1166, 1199, 1310, 1460, 1466, 1484
ZraI GACGTC 1 cut(s) 7
ZrmI AGTACT 1 cut(s) 872
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.