FvH4_3g11730

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000066
Physical Location & Seq
Reverse (-)
6923926 .. 6927650
3725 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g11730.t1

Sequence Viewer

Length: 2337 bp
ATGGTCTTACACCGGAGAATGCTCATTTCGCAGCTGATCTCATTGGTAGTGGTGGCCATAGCTGCAGTAGCAGTAGCCAACGAAGCCCTCCCGCCTCCTCAAGCCCTGCCTGGATGCTCTGGCCACTGTGGCAATCTCACTGTTCCCTATCCGTTCGGCATAGGCGACGGTTGTTACCTTGAACCTCGGTTCAACCTCACCTGTAACGAATCCACCCAACCTCCAACACTACTCTGGGGGGAAGGTATTATTACTACTAACATATCCGTGCTCGAAGGTGAGATGCAAATTCTGGCTTACATAGCCAACGATTGTTACGACCAACAGGGATATCAAACGGAAGCCATGAGGAATTCCCCACTTCTCTGGGTTAATCCTCCGTTTACCATCTCTGGTACCAGAAACAGGTTCGTCGCCGTTGGCTATGACACATATGCCATATTTACAGGGTACCGTCATTACCCGAATGATCATTCTAGGTACGTAGCCGGGTGCATGTCCATAAGTGACAGTCTTGACAATACTACTACTGCTGCTGGTAACGAGTTATGCTCTGGCATTGGATGTTGCACAACTTCAGTCCCTAACGGACTCAAGAATCGTACTATCGAGTTGAACAGCTACTACAACCATACCTACGTATGGAACTTCAACCCGTGCAGCTACGCTTTCATTGTGGAAGAAGGCAAGTTCACATTTAACCCGACTACAAGTTTTCAACAACTCGACGACACTGAAATGCTTCCTATGATTCTTGACTGGGAAATCGCGGACGGGTCATGTGCTCAAGCTCAAAAGAGGCCGGATTATGCTTGCAAGGCGCATAGCGAGTGTGTCAACCGGACCATCGACATCAACACTAAACCGACTGGTTATTATTGCAAGTGCAAGCAAGGCTACGAAGGGAATCCATATCTCCCAGATGGTTGCCAAGATACTGATGAGTGCATAGCTCCAACAAACCCCTGCAACAATGGAACGTGCACAAATACAGTTGGAGGCTACACTTGTTCATGTAACAAAGGTTTGACTTTCAGAAACGAAAGCCCTAAGGGTTGCATACCGAACCCGTCTATATCAAAGAACAAGTCCCTGAAAGTTGTTTTGGGTGTCACTCTGAGCTTCGTAGGGGTATTGCTCTTAACATTTTGGATATACTGCGGACTAAAGAGAAGAAAGTTCAAGCAGCTCAAGGAGAAGTACTACAAAGACAATGGTGGCTTATTGTTACAACAAAAATTGCAGACCTACGAAGGGCATCAAGCAGCCAAAATCTTTACTATAGGAGAGCTCAAGAAGGCAACGAACAACTTTGATGACACTGAAAAGATTGGAGAAGGAGGCTACGGATTTGTGTACAAGGGAACATTGCCAGATAACAAGGAGGTTGCCATAAAGATGTCAAAGATTGATGCTCCCACGACCCAAAGCGACCAGTTTATCAACGAGGTGGTTGTTCTCTCTCAAATCAACCATAGAAATGTAGTGAGACTCTTAGGTTGTTGTTTGGAGACCAAAACGCCTCTGTTAGTGTACGAGTTCGTCAGCAATGGCACACTTTATGAGCACATTCATAAGAAGAATGGCAAGGGACCTCTTTCCTTTGCGTTACGAATGAAGATAGCAGCAGAAACAGCAGGATCCCTAGCATACTTGCACTACTCCACTTCCATGCAAATTGTGCACCGAGATGTGAAGGCAACGAACATTCTACTAGATGAGAATTTCACAGCCAAAGTATCCGATTTTGGAGCTTCGAAATTGGTTCCCGAAGATCAAAACCAATTGTCTACTTTAGTGCAAGGGACGCTAGGGTACTTGGACCCTGAATATCTTCAATCACACACACTGACGGAGAAGAGTGACGTCTATAGTTTCGGTGTTGTTCTTGTGGAGCTCATAACGAGTCAGGTGGCAATTTCTCACCAGAAGCCTGAGGCAGAGAGAAACCTAGCGAACTCTTTTCTGATGTCGCTGGAAGATAACAACTTGGATCAGATTCTTGATCTTGAGATTATCAAAGAGGGAAGCTTTGAAATAGCTGAACAAGTTGCCCATCTCGCCAAAAGATGTTTGAGTTTGAAAGGAGGGGATAGGCCTACCATGAAAGAAGTAGCGATAGAGCTAGAGGCAATATTGGCAGTTATGGCAAAGCACCCAGGGGGAAAGCCTGACTCCTCCCCGAAAGAGACCGACTACTTGCTTGCTCCGTCACCTTCAAATGCTTACAATGTTGATGTTAGAGGTGATGAAGATGAGGTCATAACCAGCATAGATTACGACCAGAGTATGCAGAATCAATCCCAAATGATGAAGCCTTATGACGGCGGGCGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK cEGF

Protein Analysis

779

Amino Acids

86.03

Weight (kDa)

5.45

Isoelectric Point (pI)

37.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 39 - 92 7.7e-10 Wall-associated receptor kinase galacturonan-binding
EGF_CA PF07645 312 - 353 1e-07 Calcium-binding EGF domain
Pkinase PF00069 439 - 705 1e-48 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 441 - 707 2.6e-48 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21210 AT1G21230 AT1G21240 AT1G21240 AT1G21245 AT1G21250 AT1G21270 AT1G22720 AT3G25490
fragaria_vesca FvH4_2g35051 FvH4_2g35051 FvH4_2g35060 FvH4_3g10281 FvH4_3g10285 FvH4_3g10286 FvH4_3g10311 FvH4_3g10311 FvH4_3g10320 FvH4_3g10332 FvH4_3g10340 FvH4_3g10350 FvH4_3g10360 FvH4_3g10390 FvH4_3g10412 FvH4_3g10413 FvH4_3g10461 FvH4_3g10462 FvH4_3g10470 FvH4_3g10510 FvH4_3g10520 FvH4_3g10530 FvH4_3g10550 FvH4_3g10560 FvH4_3g10611 FvH4_3g11730 FvH4_6g53140 FvH4_6g53142 FvH4_7g31880 FvH4_7g31910 FvH4_7g31920
malus_domestica MD05G1269900.v1.1 MD05G1270000.v1.1 MD05G1270200.v1.1 MD05G1270400.v1.1 MD05G1270500.v1.1 MD05G1270600.v1.1 MD05G1270800.v1.1 MD05G1270900.v1.1 MD07G1183800.v1.1 MD08G1107600.v1.1 MD10G1248500.v1.1 MD10G1249200.v1.1 MD10G1249500.v1.1 MD10G1250000.v1.1 MD10G1250500.v1.1 MD10G1250900.v1.1 MD10G1251200.v1.1 MD10G1251400.v1.1 MD13G1004600.v1.1 MD15G1088400.v1.1
prunus_persica Prupe.1G442400_v2.0.a1 Prupe.1G442500_v2.0.a1 Prupe.2G223400_v2.0.a1 Prupe.4G091900_v2.0.a1 Prupe.4G093200_v2.0.a1 Prupe.4G093300_v2.0.a1 Prupe.4G093400_v2.0.a1 Prupe.4G093500_v2.0.a1 Prupe.4G094100_v2.0.a1 Prupe.4G094200_v2.0.a1 Prupe.4G094300_v2.0.a1 Prupe.6G358300_v2.0.a1
pyrus_communis pycom08g08990 pycom10g20660 pycom10g20670 pycom10g20680 pycom10g20710 pycom10g20740 pycom13g00400 pycom15g08290
rosa_chinensis RchiOBHm_Chr1g0326861 RchiOBHm_Chr1g0326871 RchiOBHm_Chr1g0331391 RchiOBHm_Chr1g0336911 RchiOBHm_Chr1g0364001 RchiOBHm_Chr2g0174541 RchiOBHm_Chr2g0175041 RchiOBHm_Chr2g0175101 RchiOBHm_Chr2g0175121 RchiOBHm_Chr3g0486331 RchiOBHm_Chr5g0015581 RchiOBHm_Chr5g0016551 RchiOBHm_Chr5g0016561 RchiOBHm_Chr5g0016581 RchiOBHm_Chr5g0016601 RchiOBHm_Chr5g0016611 RchiOBHm_Chr5g0016641 RchiOBHm_Chr5g0016661 RchiOBHm_Chr5g0016691 RchiOBHm_Chr5g0016701 RchiOBHm_Chr5g0016731 RchiOBHm_Chr5g0016741 RchiOBHm_Chr5g0016811 RchiOBHm_Chr5g0016841 RchiOBHm_Chr5g0016871 RchiOBHm_Chr5g0016881 RchiOBHm_Chr5g0016931 RchiOBHm_Chr5g0017021 RchiOBHm_Chr5g0017041 RchiOBHm_Chr5g0017071 RchiOBHm_Chr5g0017091 RchiOBHm_Chr5g0017121 RchiOBHm_Chr5g0017141 RchiOBHm_Chr5g0017291 RchiOBHm_Chr5g0017321 RchiOBHm_Chr5g0017401 RchiOBHm_Chr5g0019001 RchiOBHm_Chr5g0019071 RchiOBHm_Chr5g0028941 RchiOBHm_Chr5g0052141 RchiOBHm_Chr5g0054721 RchiOBHm_Chr5g0054801 RchiOBHm_Chr5g0054931 RchiOBHm_Chr5g0054941 RchiOBHm_Chr6g0259291 RchiOBHm_Chr6g0292991 RchiOBHm_Chr6g0306911
rosa_laevigata RLG00000009465 RLG00000009468 RLG00000010755 RLG00000011976 RLG00000014541 RLG00000014542 RLG00000015169 RLG00000022308 RLG00000027529 RLG00000029324 RLG00000030244 RLG00000030245 RLG00000032210 RLG00000032211 RLG00000032257 RLG00000032259 RLG00000032260 RLG00000032261 RLG00000032262 RLG00000032268 RLG00000032269 RLG00000032272 RLG00000032282 RLG00000032283 RLG00000032308 RLG00000032413 RLG00000033129 RLG00000034945
rosa_multiflora Rmu_co8241929.1_g000001 Rmu_co8269227.1_g000001 Rmu_co8461259.1_g000001 Rmu_co8495385.1_g000001 Rmu_sc0000028.1_g000003 Rmu_sc0000083.1_g000009 Rmu_sc0000083.1_g000010 Rmu_sc0000083.1_g000031 Rmu_sc0000083.1_g000043 Rmu_sc0000083.1_g000046 Rmu_sc0000083.1_g000047 Rmu_sc0000563.1_g000024 Rmu_sc0000842.1_g000012 Rmu_sc0001235.1_g000001 Rmu_sc0001235.1_g000005 Rmu_sc0001235.1_g000009 Rmu_sc0001235.1_g000015 Rmu_sc0001235.1_g000016 Rmu_sc0001235.1_g000017 Rmu_sc0001473.1_g000036 Rmu_sc0001607.1_g000016 Rmu_sc0001788.1_g000006 Rmu_sc0002018.1_g000008 Rmu_sc0002028.1_g000001 Rmu_sc0002680.1_g000011 Rmu_sc0002798.1_g000023 Rmu_sc0002886.1_g000023 Rmu_sc0002989.1_g000012 Rmu_sc0003064.1_g000009 Rmu_sc0003382.1_g000006 Rmu_sc0003630.1_g000002 Rmu_sc0003630.1_g000047 Rmu_sc0003630.1_g000066 Rmu_sc0003630.1_g000067 Rmu_sc0003649.1_g000010 Rmu_sc0003688.1_g000005 Rmu_sc0003920.1_g000007 Rmu_sc0004765.1_g000007 Rmu_sc0004765.1_g000008 Rmu_sc0004920.1_g000011 Rmu_sc0005223.1_g000008 Rmu_sc0005223.1_g000010 Rmu_sc0005223.1_g000015 Rmu_sc0005223.1_g000016 Rmu_sc0005319.1_g000014 Rmu_sc0005319.1_g000015 Rmu_sc0005319.1_g000018 Rmu_sc0005319.1_g000019 Rmu_sc0005319.1_g000025 Rmu_sc0005418.1_g000007 Rmu_sc0005418.1_g000012 Rmu_sc0006106.1_g000011 Rmu_sc0006914.1_g000012 Rmu_sc0007023.1_g000001 Rmu_sc0007662.1_g000001 Rmu_sc0007920.1_g000002 Rmu_sc0007920.1_g000013 Rmu_sc0008053.1_g000016 Rmu_sc0008053.1_g000017 Rmu_sc0008053.1_g000018 Rmu_sc0008053.1_g000023 Rmu_sc0008053.1_g000050 Rmu_sc0009042.1_g000007 Rmu_sc0009042.1_g000008 Rmu_sc0010642.1_g000008 Rmu_sc0011109.1_g000002 Rmu_sc0011314.1_g000006 Rmu_sc0011974.1_g000006 Rmu_sc0012179.1_g000009 Rmu_sc0015169.1_g000001 Rmu_sc0016368.1_g000002 Rmu_sc0022659.1_g000001 Rmu_sc0027371.1_g000001 Rmu_sc0028213.1_g000001 Rmu_sc0034526.1_g000001 Rmu_sc0039366.1_g000002 Rmu_ssc0000050.1_g000084 Rmu_ssc0000050.1_g000088 Rmu_ssc0000167.1_g000017
rosa_roxburghii Rroxscaffold_1G00011340 Rroxscaffold_1G00025710 Rroxscaffold_1G00025870 Rroxscaffold_1G00026070 Rroxscaffold_1G00026170 Rroxscaffold_1G00026200 Rroxscaffold_1G00026270 Rroxscaffold_1G00050950 Rroxscaffold_1G00058900 Rroxscaffold_1G00060040 Rroxscaffold_1G00060060 Rroxscaffold_1G00060070 Rroxscaffold_1G00060080 Rroxscaffold_1G00060110 Rroxscaffold_1G00060120 Rroxscaffold_1G00060140 Rroxscaffold_1G00060170 Rroxscaffold_1G00060210 Rroxscaffold_1G00060220 Rroxscaffold_1G00060240 Rroxscaffold_1G00060250 Rroxscaffold_1G00060260 Rroxscaffold_1G00060300 Rroxscaffold_1G00060320 Rroxscaffold_1G00060330 Rroxscaffold_1G00060900 Rroxscaffold_1G00060920 Rroxscaffold_1G00073980 Rroxscaffold_2G00077560 Rroxscaffold_2G00077580 Rroxscaffold_2G00146990 Rroxscaffold_3G00236200 Rroxscaffold_3G00236210 Rroxscaffold_3G00236220 Rroxscaffold_4G00292760 Rroxscaffold_4G00315200 Rroxscaffold_4G00315240 Rroxscaffold_4G00326240 Rroxscaffold_4G00328980 Rroxscaffold_5G00341890 Rroxscaffold_7G00161350 Rroxscaffold_7G00174490 Rroxscaffold_7G00206550
rosa_rugosa Rorug01G0093000 Rorug01G0093100 Rorug01G0128800 Rorug01G0312000 Rorug01G0312000 Rorug02G0581100 Rorug03G0224600 Rorug05G0028500 Rorug05G0032700 Rorug05G0032800 Rorug05G0032900 Rorug05G0032900 Rorug05G0033000 Rorug05G0033100 Rorug05G0033300 Rorug05G0033400 Rorug05G0033900 Rorug05G0034900 Rorug05G0035000 Rorug05G0046900 Rorug05G0047500 Rorug05G0047500 Rorug05G0111600 Rorug05G0287900 Rorug05G0288700 Rorug05G0586900 Rorug06G0231700 Rorug06G0354300 Rorug06G0354400
rosa_samantha Rh1DG122200 Rh2DG686800 Rh5BG136600 Rh6AG344500 Rh6BG351900 Rh6CG359000 Rh6CG359100 Rh6CG387600 Rh6CG480500 Rh6DG344600 Rh6DG467400
rosa_wichuraiana Rw0G010250 Rw0G014820 Rw0G021400 Rw0G023100 Rw1G009450 Rw1G012100 Rw1G012180 Rw1G028360 Rw2G054260 Rw3G024370 Rw3G024420 Rw3G024470 Rw5G010850 Rw5G011010 Rw5G011020 Rw5G011050 Rw5G011080 Rw5G011120 Rw5G012300 Rw5G018470 Rw5G033830 Rw6G008930 Rw6G030070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1869
Acc65I GGTACC 2 cut(s) 395, 450
AccB1I GGYRCC 2 cut(s) 395, 450
AccI GTMKAC 1 cut(s) 1790
AccII CGCG 1 cut(s) 770
AciI CCGC 4 cut(s) 92, 770, 1161, 2328
AclWI GGATC 3 cut(s) 1635, 1648, 2001
AcoI YGGCCR 2 cut(s) 54, 121
AcsI RAATTY 3 cut(s) 288, 352, 1723
AcuI CTGAAG 1 cut(s) 561
AcyI GRCGYC 1 cut(s) 1866
AfaI GTAC 8 cut(s) 397, 452, 482, 604, 1202, 1358, 1535, 1817
AfiI CCNNNNNNNGG 4 cut(s) 405, 642, 1254, 2324
AjnI CCWGG 2 cut(s) 109, 2158
AjuI GAANNNNNNNTTGG 2 cut(s) 1088, 1120
Alw21I GWGCWC 7 cut(s) 273, 787, 986, 1293, 1569, 1686, 1899
Alw26I GTCTC 3 cut(s) 1483, 1505, 2183
Alw44I GTGCAC 2 cut(s) 982, 1682
AlwI GGATC 3 cut(s) 1635, 1648, 2001
AoxI GGCC 4 cut(s) 54, 121, 800, 2096
ApaLI GTGCAC 2 cut(s) 982, 1682
ApeKI GCWGC 7 cut(s) 31, 62, 533, 660, 1186, 1265, 1625
ApoI RAATTY 3 cut(s) 288, 352, 1723
ArsI GACNNNNNNTTYG 2 cut(s) 1073, 1105
Asp700I GAANNNNTTC 2 cut(s) 741, 1833
Asp718I GGTACC 2 cut(s) 395, 450
AspLEI GCGC 1 cut(s) 823
AspS9I GGNCC 3 cut(s) 843, 1592, 1822
AsuC2I CCSGG 1 cut(s) 490
AsuHPI GGTGA 5 cut(s) 190, 290, 1916, 2205, 2258
AsuII TTCGAA 1 cut(s) 1757
AvaII GGWCC 3 cut(s) 843, 1592, 1822
AxyI CCTNAGG 2 cut(s) 1050, 1935
BaeGI GKGCMC 2 cut(s) 986, 1686
BalI TGGCCA 2 cut(s) 56, 123
BamHI GGATCC 1 cut(s) 1640
BanI GGYRCC 2 cut(s) 395, 450
BanII GRGCYC 2 cut(s) 1293, 1899
Bbv12I GWGCWC 7 cut(s) 273, 787, 986, 1293, 1569, 1686, 1899
BbvI GCAGC 7 cut(s) 43, 49, 520, 672, 1198, 1277, 1637
BccI CCATC 4 cut(s) 395, 854, 917, 2064
BceAI ACGGC 1 cut(s) 401
BciT130I CCWGG 2 cut(s) 111, 2160
BciVI GTATCC 1 cut(s) 1750
BclI TGATCA 1 cut(s) 469
BcnI CCSGG 1 cut(s) 490
BcoDI GTCTC 3 cut(s) 1483, 1505, 2183
BfaI CTAG 6 cut(s) 477, 1646, 1715, 1811, 1952, 2126
BfmI CTRYAG 3 cut(s) 63, 1281, 1870
BfuI GTATCC 1 cut(s) 1750
BglI GCCNNNNNGGC 1 cut(s) 129
BisI GCNGC 7 cut(s) 32, 63, 534, 661, 1187, 1266, 1626
BlsI GCNGC 7 cut(s) 33, 64, 535, 662, 1188, 1267, 1627
BmcAI AGTACT 1 cut(s) 1202
Bme1390I CCNGG 3 cut(s) 111, 490, 2160
Bme18I GGWCC 3 cut(s) 843, 1592, 1822
BmgT120I GGNCC 3 cut(s) 843, 1592, 1822
BmiI GGNNCC 6 cut(s) 397, 452, 1593, 1642, 1767, 1824
BmrFI CCNGG 3 cut(s) 111, 490, 2160
BmrI ACTGGG 1 cut(s) 769
BmsI GCATC 4 cut(s) 104, 273, 1267, 1402
BmuI ACTGGG 1 cut(s) 769
BplI GAGNNNNNCTC 2 cut(s) 536, 568
Bpu14I TTCGAA 1 cut(s) 1757
BpuEI CTTGAG 6 cut(s) 84, 578, 771, 1175, 1277, 2030
BpuMI CCSGG 1 cut(s) 490
BsaAI YACGTR 2 cut(s) 484, 640
BsaHI GRCGYC 1 cut(s) 1866
BsaI GGTCTC 2 cut(s) 1505, 2183
BsaJI CCNNGG 3 cut(s) 185, 2158, 2159
BsaWI WCCGGW 2 cut(s) 12, 840
BsaXI ACNNNNNCTCC 2 cut(s) 205, 235
Bsc4I CCNNNNNNNGG 4 cut(s) 405, 642, 1254, 2324
Bse1I ACTGG 3 cut(s) 764, 874, 1435
Bse21I CCTNAGG 2 cut(s) 1050, 1935
Bse3DI GCAATG 2 cut(s) 1367, 1555
BseBI CCWGG 2 cut(s) 111, 2160
BseDI CCNNGG 3 cut(s) 185, 2158, 2159
BseGI GGATG 2 cut(s) 119, 569
BseLI CCNNNNNNNGG 4 cut(s) 405, 642, 1254, 2324
BseMI GCAATG 2 cut(s) 1367, 1555
BseMII CTCAG 2 cut(s) 1109, 1926
BseNI ACTGG 3 cut(s) 764, 874, 1435
BseRI GAGGAG 2 cut(s) 87, 2167
BseSI GKGCMC 2 cut(s) 986, 1686
BseXI GCAGC 7 cut(s) 43, 49, 520, 672, 1198, 1277, 1637
BsgI GTGCAG 1 cut(s) 679
Bsh1236I CGCG 1 cut(s) 770
BshFI GGCC 4 cut(s) 56, 123, 802, 2098
BshNI GGYRCC 2 cut(s) 395, 450
BsiHKAI GWGCWC 7 cut(s) 273, 787, 986, 1293, 1569, 1686, 1899
BsiSI CCGG 4 cut(s) 13, 489, 803, 841
BslFI GGGAC 4 cut(s) 566, 1075, 1605, 1819
BslI CCNNNNNNNGG 4 cut(s) 405, 642, 1254, 2324
BsmAI GTCTC 3 cut(s) 1483, 1505, 2183
BsmFI GGGAC 4 cut(s) 566, 1075, 1605, 1819
BsmI GAATGC 1 cut(s) 24
BsnI GGCC 4 cut(s) 56, 123, 802, 2098
Bso31I GGTCTC 2 cut(s) 1505, 2183
Bsp119I TTCGAA 1 cut(s) 1757
Bsp1286I GDGCHC 7 cut(s) 273, 787, 986, 1293, 1569, 1686, 1899
Bsp1407I TGTACA 1 cut(s) 1356
Bsp143I GATC 6 cut(s) 36, 469, 1640, 1774, 1993, 2005
BspACI CCGC 4 cut(s) 92, 770, 1161, 2328
BspANI GGCC 4 cut(s) 56, 123, 802, 2098
BspCNI CTCAG 2 cut(s) 1110, 1927
BspFNI CGCG 1 cut(s) 770
BspLI GGNNCC 6 cut(s) 397, 452, 1593, 1642, 1767, 1824
BspMAI CTGCAG 1 cut(s) 67
BspPI GGATC 3 cut(s) 1635, 1648, 2001
BspT104I TTCGAA 1 cut(s) 1757
BspT107I GGYRCC 2 cut(s) 395, 450
BspTNI GGTCTC 2 cut(s) 1505, 2183
BsrDI GCAATG 2 cut(s) 1367, 1555
BsrGI TGTACA 1 cut(s) 1356
BsrI ACTGG 3 cut(s) 764, 874, 1435
BssECI CCNNGG 3 cut(s) 185, 2158, 2159
BssMI GATC 6 cut(s) 36, 469, 1640, 1774, 1993, 2005
BssNI GRCGYC 1 cut(s) 1866
Bst2UI CCWGG 2 cut(s) 111, 2160
Bst4CI ACNGT 6 cut(s) 128, 142, 170, 455, 512, 994
Bst6I CTCTTC 1 cut(s) 1853
BstACI GRCGYC 1 cut(s) 1866
BstAPI GCANNNNNTGC 1 cut(s) 1681
BstAUI TGTACA 1 cut(s) 1356
BstBAI YACGTR 2 cut(s) 484, 640
BstBI TTCGAA 1 cut(s) 1757
BstC8I GCNNGC 4 cut(s) 814, 890, 2205, 2330
BstDEI CTNAG 4 cut(s) 1050, 1118, 1495, 1935
BstF5I GGATG 2 cut(s) 119, 569
BstFNI CGCG 1 cut(s) 770
BstHHI GCGC 1 cut(s) 823
BstKTI GATC 6 cut(s) 39, 472, 1643, 1777, 1996, 2008
BstMAI GTCTC 3 cut(s) 1483, 1505, 2183
BstMBI GATC 6 cut(s) 36, 469, 1640, 1774, 1993, 2005
BstNI CCWGG 2 cut(s) 111, 2160
BstNSI RCATGY 1 cut(s) 499
BstSCI CCNGG 3 cut(s) 109, 488, 2158
BstSFI CTRYAG 3 cut(s) 63, 1281, 1870
BstSLI GKGCMC 2 cut(s) 986, 1686
BstSNI TACGTA 2 cut(s) 484, 640
BstUI CGCG 1 cut(s) 770
BstV1I GCAGC 7 cut(s) 43, 49, 520, 672, 1198, 1277, 1637
BstX2I RGATCY 1 cut(s) 1640
BstXI CCANNNNNNTGG 1 cut(s) 366
BstYI RGATCY 1 cut(s) 1640
Bsu36I CCTNAGG 2 cut(s) 1050, 1935
BsuI GTATCC 1 cut(s) 1750
BsuRI GGCC 4 cut(s) 56, 123, 802, 2098
BtsCI GGATG 2 cut(s) 119, 569
BtsIMutI CAGTG 5 cut(s) 124, 138, 732, 1320, 1847
Cac8I GCNNGC 4 cut(s) 814, 890, 2205, 2330
CfoI GCGC 1 cut(s) 823
Cfr13I GGNCC 3 cut(s) 843, 1592, 1822
CseI GACGC 1 cut(s) 1816
Csp6I GTAC 8 cut(s) 396, 451, 481, 603, 1201, 1357, 1534, 1816
CviAII CATG 6 cut(s) 346, 496, 780, 1014, 1672, 2104
CviQI GTAC 8 cut(s) 396, 451, 481, 603, 1201, 1357, 1534, 1816
DdeI CTNAG 4 cut(s) 1050, 1118, 1495, 1935
DpnI GATC 6 cut(s) 38, 471, 1642, 1776, 1995, 2007
DpnII GATC 6 cut(s) 36, 469, 1640, 1774, 1993, 2005
EaeI YGGCCR 2 cut(s) 54, 121
Eam1104I CTCTTC 1 cut(s) 1853
EarI CTCTTC 1 cut(s) 1853
Ecl136II GAGCTC 2 cut(s) 1291, 1897
Eco105I TACGTA 2 cut(s) 484, 640
Eco147I AGGCCT 1 cut(s) 2098
Eco24I GRGCYC 2 cut(s) 1293, 1899
Eco31I GGTCTC 2 cut(s) 1505, 2183
Eco32I GATATC 1 cut(s) 332
Eco47I GGWCC 3 cut(s) 843, 1592, 1822
Eco53kI GAGCTC 2 cut(s) 1291, 1897
Eco57I CTGAAG 1 cut(s) 561
Eco81I CCTNAGG 2 cut(s) 1050, 1935
EcoICRI GAGCTC 2 cut(s) 1291, 1897
EcoO109I RGGNCCY 1 cut(s) 1592
EcoRI GAATTC 1 cut(s) 352
EcoRII CCWGG 2 cut(s) 109, 2158
EcoRV GATATC 1 cut(s) 332
EcoT38I GRGCYC 2 cut(s) 1293, 1899
FaeI CATG 6 cut(s) 349, 499, 783, 1017, 1675, 2107
FaqI GGGAC 4 cut(s) 566, 1075, 1605, 1819
FatI CATG 6 cut(s) 345, 495, 779, 1013, 1671, 2103
FauI CCCGC 2 cut(s) 99, 2321
FauNDI CATATG 1 cut(s) 433
FbaI TGATCA 1 cut(s) 469
FblI GTMKAC 1 cut(s) 1790
Fnu4HI GCNGC 7 cut(s) 32, 63, 534, 661, 1187, 1266, 1626
FokI GGATG 2 cut(s) 126, 576
FriOI GRGCYC 2 cut(s) 1293, 1899
Fsp4HI GCNGC 7 cut(s) 32, 63, 534, 661, 1187, 1266, 1626
FspBI CTAG 6 cut(s) 477, 1646, 1715, 1811, 1952, 2126
GlaI GCGC 1 cut(s) 822
GluI GCNGC 7 cut(s) 32, 63, 534, 661, 1187, 1266, 1626
HaeIII GGCC 4 cut(s) 56, 123, 802, 2098
HapII CCGG 4 cut(s) 13, 489, 803, 841
HgaI GACGC 1 cut(s) 1816
HhaI GCGC 1 cut(s) 823
Hin1I GRCGYC 1 cut(s) 1866
Hin1II CATG 6 cut(s) 349, 499, 783, 1017, 1675, 2107
Hin6I GCGC 1 cut(s) 821
HinP1I GCGC 1 cut(s) 821
HincII GTYRAC 1 cut(s) 838
HindII GTYRAC 1 cut(s) 838
HindIII AAGCTT 1 cut(s) 2029
HpaII CCGG 4 cut(s) 13, 489, 803, 841
HphI GGTGA 5 cut(s) 190, 290, 1916, 2205, 2258
Hpy166II GTNNAC 8 cut(s) 384, 693, 838, 984, 1357, 1534, 1684, 1791
Hpy188I TCNGA 5 cut(s) 1037, 1119, 1744, 1968, 1998
Hpy188III TCNNGA 7 cut(s) 515, 595, 755, 1294, 1769, 2003, 2009
Hpy8I GTNNAC 8 cut(s) 384, 693, 838, 984, 1357, 1534, 1684, 1791
Hpy99I CGWCG 3 cut(s) 170, 416, 731
HpyAV CCTTC 9 cut(s) 236, 269, 677, 896, 1247, 1291, 1331, 1690, 2226
HpyCH4III ACNGT 6 cut(s) 128, 142, 170, 455, 512, 994
HpyCH4IV ACGT 4 cut(s) 483, 639, 980, 1866
HpyF3I CTNAG 4 cut(s) 1050, 1118, 1495, 1935
HpySE526I ACGT 4 cut(s) 483, 639, 980, 1866
Hsp92I GRCGYC 1 cut(s) 1866
Hsp92II CATG 6 cut(s) 349, 499, 783, 1017, 1675, 2107
HspAI GCGC 1 cut(s) 821
KpnI GGTACC 2 cut(s) 399, 454
Ksp22I TGATCA 1 cut(s) 469
Kzo9I GATC 6 cut(s) 36, 469, 1640, 1774, 1993, 2005
LmnI GCTCC 5 cut(s) 958, 1420, 1751, 1894, 2212
Lsp1109I GCAGC 7 cut(s) 43, 49, 520, 672, 1198, 1277, 1637
LweI GCATC 4 cut(s) 104, 273, 1267, 1402
MaeI CTAG 6 cut(s) 477, 1646, 1715, 1811, 1952, 2126
MaeII ACGT 4 cut(s) 483, 639, 980, 1866
MalI GATC 6 cut(s) 38, 471, 1642, 1776, 1995, 2007
MboI GATC 6 cut(s) 36, 469, 1640, 1774, 1993, 2005
MboII GAAGA 9 cut(s) 692, 1185, 1591, 1630, 1784, 1826, 1870, 1991, 2264
MfeI CAATTG 1 cut(s) 1784
MflI RGATCY 1 cut(s) 1640
MhlI GDGCHC 7 cut(s) 273, 787, 986, 1293, 1569, 1686, 1899
MlsI TGGCCA 2 cut(s) 56, 123
MluCI AATT 8 cut(s) 288, 352, 1238, 1677, 1723, 1760, 1784, 1917
MluNI TGGCCA 2 cut(s) 56, 123
MlyI GAGTC 4 cut(s) 585, 1485, 1915, 2168
MmeI TCCRAC 3 cut(s) 248, 976, 980
Mox20I TGGCCA 2 cut(s) 56, 123
MroXI GAANNNNTTC 2 cut(s) 741, 1833
MscI TGGCCA 2 cut(s) 56, 123
MseI TTAA 3 cut(s) 372, 699, 1142
MslI CAYNNNNRTG 6 cut(s) 266, 737, 1397, 1479, 1670, 1689
Msp20I TGGCCA 2 cut(s) 56, 123
MspA1I CMGCKG 1 cut(s) 34
MspI CCGG 4 cut(s) 13, 489, 803, 841
MspR9I CCNGG 3 cut(s) 111, 490, 2160
MunI CAATTG 1 cut(s) 1784
Mva1269I GAATGC 1 cut(s) 24
MvaI CCWGG 2 cut(s) 111, 2160
MvnI CGCG 1 cut(s) 770
NciI CCSGG 1 cut(s) 490
NdeI CATATG 1 cut(s) 433
NdeII GATC 6 cut(s) 36, 469, 1640, 1774, 1993, 2005
NlaIII CATG 6 cut(s) 349, 499, 783, 1017, 1675, 2107
NlaIV GGNNCC 6 cut(s) 397, 452, 1593, 1642, 1767, 1824
NmuCI GTSAC 4 cut(s) 506, 1111, 1862, 2211
NspI RCATGY 1 cut(s) 499
NspV TTCGAA 1 cut(s) 1757
PasI CCCWGGG 1 cut(s) 2159
PceI AGGCCT 1 cut(s) 2098
PcsI WCGNNNNNNNCGW 2 cut(s) 162, 315
PctI GAATGC 1 cut(s) 24
PdmI GAANNNNTTC 2 cut(s) 741, 1833
PfeI GAWTC 6 cut(s) 209, 598, 751, 907, 1999, 2296
PkrI GCNGC 7 cut(s) 33, 64, 535, 662, 1188, 1267, 1627
PleI GAGTC 4 cut(s) 585, 1485, 1914, 2168
PpsI GAGTC 4 cut(s) 585, 1485, 1914, 2168
Ppu21I YACGTR 2 cut(s) 484, 640
PpuMI RGGWCCY 1 cut(s) 1592
Psp124BI GAGCTC 2 cut(s) 1293, 1899
Psp5II RGGWCCY 1 cut(s) 1592
Psp6I CCWGG 2 cut(s) 109, 2158
PspGI CCWGG 2 cut(s) 109, 2158
PspN4I GGNNCC 6 cut(s) 397, 452, 1593, 1642, 1767, 1824
PspPI GGNCC 3 cut(s) 843, 1592, 1822
PspPPI RGGWCCY 1 cut(s) 1592
PsrI GAACNNNNNNTAC 2 cut(s) 608, 640
PstI CTGCAG 1 cut(s) 67
PsuI RGATCY 1 cut(s) 1640
PvuII CAGCTG 1 cut(s) 34
RsaI GTAC 8 cut(s) 397, 452, 482, 604, 1202, 1358, 1535, 1817
RsaNI GTAC 8 cut(s) 396, 451, 481, 603, 1201, 1357, 1534, 1816
RseI CAYNNNNRTG 6 cut(s) 266, 737, 1397, 1479, 1670, 1689
SacI GAGCTC 2 cut(s) 1293, 1899
SaqAI TTAA 3 cut(s) 372, 699, 1142
SatI GCNGC 7 cut(s) 32, 63, 534, 661, 1187, 1266, 1626
Sau3AI GATC 6 cut(s) 36, 469, 1640, 1774, 1993, 2005
Sau96I GGNCC 3 cut(s) 843, 1592, 1822
ScaI AGTACT 1 cut(s) 1202
SchI GAGTC 4 cut(s) 585, 1485, 1915, 2168
ScrFI CCNGG 3 cut(s) 111, 490, 2160
SduI GDGCHC 7 cut(s) 273, 787, 986, 1293, 1569, 1686, 1899
SfaNI GCATC 4 cut(s) 104, 273, 1267, 1402
SfcI CTRYAG 3 cut(s) 63, 1281, 1870
SfuI TTCGAA 1 cut(s) 1757
SinI GGWCC 3 cut(s) 843, 1592, 1822
SmiMI CAYNNNNRTG 6 cut(s) 266, 737, 1397, 1479, 1670, 1689
SmlI CTYRAG 6 cut(s) 99, 593, 786, 1190, 1292, 2009
SmoI CTYRAG 6 cut(s) 99, 593, 786, 1190, 1292, 2009
SnaBI TACGTA 2 cut(s) 484, 640
Sse9I AATT 8 cut(s) 288, 352, 1238, 1677, 1723, 1760, 1784, 1917
SseBI AGGCCT 1 cut(s) 2098
SsiI CCGC 4 cut(s) 92, 770, 1161, 2328
SspI AATATT 1 cut(s) 2136
SspMI CTAG 6 cut(s) 477, 1646, 1715, 1811, 1952, 2126
SstI GAGCTC 2 cut(s) 1293, 1899
StuI AGGCCT 1 cut(s) 2098
StyD4I CCNGG 3 cut(s) 109, 488, 2158
TaaI ACNGT 6 cut(s) 128, 142, 170, 455, 512, 994
TaiI ACGT 4 cut(s) 486, 642, 983, 1869
TaqI TCGA 5 cut(s) 273, 609, 726, 849, 1757
TaqII GACCGA 1 cut(s) 2207
TasI AATT 8 cut(s) 288, 352, 1238, 1677, 1723, 1760, 1784, 1917
TatI WGTACW 2 cut(s) 1200, 1356
TfiI GAWTC 6 cut(s) 209, 598, 751, 907, 1999, 2296
Tru1I TTAA 3 cut(s) 372, 699, 1142
Tru9I TTAA 3 cut(s) 372, 699, 1142
TscAI CASTG 5 cut(s) 131, 145, 739, 1327, 1854
TseFI GTSAC 4 cut(s) 506, 1111, 1862, 2211
TseI GCWGC 7 cut(s) 31, 62, 533, 660, 1186, 1265, 1625
Tsp45I GTSAC 4 cut(s) 506, 1111, 1862, 2211
TspDTI ATGAA 7 cut(s) 661, 1002, 1562, 1631, 2120, 2265, 2327
TspGWI ACGGA 8 cut(s) 141, 256, 353, 369, 603, 1362, 1868, 2199
TspRI CASTG 5 cut(s) 131, 145, 739, 1327, 1854
VneI GTGCAC 2 cut(s) 982, 1682
VpaK11BI GGWCC 3 cut(s) 843, 1592, 1822
XapI RAATTY 3 cut(s) 288, 352, 1723
XceI RCATGY 1 cut(s) 499
XcmI CCANNNNNNNNNTGG 1 cut(s) 231
XmiI GTMKAC 1 cut(s) 1790
XmnI GAANNNNTTC 2 cut(s) 741, 1833
XspI CTAG 6 cut(s) 477, 1646, 1715, 1811, 1952, 2126
ZraI GACGTC 1 cut(s) 1867
ZrmI AGTACT 1 cut(s) 1202
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.