Wall-associated receptor kinase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 1009 |
| AatII | GACGTC | 1 cut(s) | 2310 |
| Acc36I | ACCTGC | 1 cut(s) | 1009 |
| AccI | GTMKAC | 2 cut(s) | 1362, 1372 |
| AccII | CGCG | 1 cut(s) | 1991 |
| AciI | CCGC | 8 cut(s) | 116, 145, 602, 876, 1218, 1749, 1989, 1991 |
| AclWI | GGATC | 3 cut(s) | 1684, 1787, 1944 |
| AcoI | YGGCCR | 3 cut(s) | 646, 1992, 2169 |
| AcsI | RAATTY | 5 cut(s) | 865, 994, 1098, 1143, 2047 |
| AcyI | GRCGYC | 3 cut(s) | 369, 576, 2307 |
| AfaI | GTAC | 4 cut(s) | 269, 622, 1202, 1835 |
| AfeI | AGCGCT | 1 cut(s) | 2242 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 929 |
| AflII | CTTAAG | 1 cut(s) | 1298 |
| AgsI | TTSAA | 9 cut(s) | 63, 1168, 1207, 1636, 1856, 2011, 2063, 2238, 2338 |
| AhlI | ACTAGT | 1 cut(s) | 725 |
| AjnI | CCWGG | 4 cut(s) | 372, 750, 904, 2179 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1088, 1120 |
| Alw21I | GWGCWC | 3 cut(s) | 539, 956, 1587 |
| Alw26I | GTCTC | 6 cut(s) | 43, 1165, 1259, 1523, 2100, 2196 |
| Alw44I | GTGCAC | 1 cut(s) | 535 |
| AlwI | GGATC | 3 cut(s) | 1684, 1787, 1944 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1652 |
| Ama87I | CYCGRG | 1 cut(s) | 955 |
| Aor51HI | AGCGCT | 1 cut(s) | 2242 |
| AoxI | GGCC | 7 cut(s) | 3, 646, 703, 1219, 1992, 2114, 2169 |
| ApaLI | GTGCAC | 1 cut(s) | 535 |
| ApeKI | GCWGC | 2 cut(s) | 56, 1643 |
| ApoI | RAATTY | 5 cut(s) | 865, 994, 1098, 1143, 2047 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1851 |
| AspLEI | GCGC | 1 cut(s) | 2243 |
| AspS9I | GGNCC | 2 cut(s) | 1049, 1220 |
| AsuC2I | CCSGG | 2 cut(s) | 299, 520 |
| AsuII | TTCGAA | 1 cut(s) | 1182 |
| AvaI | CYCGRG | 1 cut(s) | 955 |
| AvaII | GGWCC | 1 cut(s) | 1049 |
| BaeGI | GKGCMC | 1 cut(s) | 539 |
| BalI | TGGCCA | 1 cut(s) | 2171 |
| BbsI | GAAGAC | 1 cut(s) | 2226 |
| Bbv12I | GWGCWC | 3 cut(s) | 539, 956, 1587 |
| BbvI | GCAGC | 2 cut(s) | 68, 1630 |
| BccI | CCATC | 7 cut(s) | 268, 437, 695, 1040, 2036, 2141, 2345 |
| BceAI | ACGGC | 5 cut(s) | 205, 443, 533, 661, 1585 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 559, 593, 1740, 1774 |
| BciT130I | CCWGG | 4 cut(s) | 374, 752, 906, 2181 |
| BclI | TGATCA | 1 cut(s) | 97 |
| BcnI | CCSGG | 2 cut(s) | 299, 520 |
| BcoDI | GTCTC | 6 cut(s) | 43, 1165, 1259, 1523, 2100, 2196 |
| BcuI | ACTAGT | 1 cut(s) | 725 |
| BfaI | CTAG | 3 cut(s) | 726, 1232, 1733 |
| BfmI | CTRYAG | 2 cut(s) | 1287, 1644 |
| BfoI | RGCGCY | 1 cut(s) | 2244 |
| BfrI | CTTAAG | 1 cut(s) | 1298 |
| BfuAI | ACCTGC | 1 cut(s) | 1009 |
| BisI | GCNGC | 5 cut(s) | 57, 877, 1219, 1644, 1992 |
| BlsI | GCNGC | 5 cut(s) | 58, 878, 1220, 1645, 1993 |
| BmcAI | AGTACT | 1 cut(s) | 1202 |
| Bme1390I | CCNGG | 6 cut(s) | 299, 374, 520, 752, 906, 2181 |
| Bme18I | GGWCC | 1 cut(s) | 1049 |
| BmeT110I | CYCGRG | 1 cut(s) | 955 |
| BmgT120I | GGNCC | 2 cut(s) | 1049, 1220 |
| BmiI | GGNNCC | 1 cut(s) | 1785 |
| BmrFI | CCNGG | 6 cut(s) | 299, 374, 520, 752, 906, 2181 |
| BmsI | GCATC | 3 cut(s) | 189, 240, 1781 |
| BpiI | GAAGAC | 1 cut(s) | 2226 |
| BpmI | CTGGAG | 2 cut(s) | 292, 418 |
| Bpu14I | TTCGAA | 1 cut(s) | 1182 |
| BpuEI | CTTGAG | 5 cut(s) | 105, 579, 1283, 1376, 2246 |
| BpuMI | CCSGG | 2 cut(s) | 299, 520 |
| Bsa29I | ATCGAT | 1 cut(s) | 1688 |
| BsaBI | GATNNNNATC | 2 cut(s) | 1694, 1797 |
| BsaHI | GRCGYC | 3 cut(s) | 369, 576, 2307 |
| BsaJI | CCNNGG | 7 cut(s) | 372, 373, 762, 1052, 1953, 1989, 2180 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1033, 1063 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 929 |
| Bse3DI | GCAATG | 1 cut(s) | 886 |
| Bse8I | GATNNNNATC | 2 cut(s) | 1694, 1797 |
| BseBI | CCWGG | 4 cut(s) | 374, 752, 906, 2181 |
| BseCI | ATCGAT | 1 cut(s) | 1688 |
| BseDI | CCNNGG | 7 cut(s) | 372, 373, 762, 1052, 1953, 1989, 2180 |
| BseGI | GGATG | 7 cut(s) | 27, 331, 747, 1402, 1772, 2047, 2152 |
| BseJI | GATNNNNATC | 2 cut(s) | 1694, 1797 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 929 |
| BseMI | GCAATG | 1 cut(s) | 886 |
| BseSI | GKGCMC | 1 cut(s) | 539 |
| BseX3I | CGGCCG | 1 cut(s) | 1992 |
| BseXI | GCAGC | 2 cut(s) | 68, 1630 |
| Bsh1236I | CGCG | 1 cut(s) | 1991 |
| Bsh1285I | CGRYCG | 2 cut(s) | 145, 1995 |
| BshFI | GGCC | 7 cut(s) | 5, 648, 705, 1221, 1994, 2116, 2171 |
| BshVI | ATCGAT | 1 cut(s) | 1688 |
| BsiEI | CGRYCG | 2 cut(s) | 145, 1995 |
| BsiHKAI | GWGCWC | 3 cut(s) | 539, 956, 1587 |
| BsiHKCI | CYCGRG | 1 cut(s) | 955 |
| BsiSI | CCGG | 3 cut(s) | 209, 298, 519 |
| BslFI | GGGAC | 3 cut(s) | 584, 791, 1837 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 929 |
| BsmAI | GTCTC | 6 cut(s) | 43, 1165, 1259, 1523, 2100, 2196 |
| BsmBI | CGTCTC | 2 cut(s) | 1165, 2100 |
| BsmFI | GGGAC | 3 cut(s) | 584, 791, 1837 |
| BsnI | GGCC | 7 cut(s) | 5, 648, 705, 1221, 1994, 2116, 2171 |
| BsoBI | CYCGRG | 1 cut(s) | 955 |
| Bsp119I | TTCGAA | 1 cut(s) | 1182 |
| Bsp1286I | GDGCHC | 3 cut(s) | 539, 956, 1587 |
| Bsp143I | GATC | 5 cut(s) | 97, 1440, 1689, 1792, 1949 |
| Bsp19I | CCATGG | 2 cut(s) | 762, 1052 |
| BspACI | CCGC | 8 cut(s) | 116, 145, 602, 876, 1218, 1749, 1989, 1991 |
| BspANI | GGCC | 7 cut(s) | 5, 648, 705, 1221, 1994, 2116, 2171 |
| BspDI | ATCGAT | 1 cut(s) | 1688 |
| BspFNI | CGCG | 1 cut(s) | 1991 |
| BspHI | TCATGA | 1 cut(s) | 339 |
| BspLI | GGNNCC | 1 cut(s) | 1785 |
| BspMAI | CTGCAG | 2 cut(s) | 1291, 1648 |
| BspMI | ACCTGC | 1 cut(s) | 1009 |
| BspPI | GGATC | 3 cut(s) | 1684, 1787, 1944 |
| BspT104I | TTCGAA | 1 cut(s) | 1182 |
| BspTI | CTTAAG | 1 cut(s) | 1298 |
| BsrDI | GCAATG | 1 cut(s) | 886 |
| BssECI | CCNNGG | 7 cut(s) | 372, 373, 762, 1052, 1953, 1989, 2180 |
| BssMI | GATC | 5 cut(s) | 97, 1440, 1689, 1792, 1949 |
| BssNAI | GTATAC | 1 cut(s) | 1373 |
| BssNI | GRCGYC | 3 cut(s) | 369, 576, 2307 |
| BssT1I | CCWWGG | 2 cut(s) | 762, 1052 |
| Bst1107I | GTATAC | 1 cut(s) | 1373 |
| Bst2UI | CCWGG | 4 cut(s) | 374, 752, 906, 2181 |
| Bst4CI | ACNGT | 3 cut(s) | 204, 620, 625 |
| Bst6I | CTCTTC | 3 cut(s) | 713, 1953, 2206 |
| BstACI | GRCGYC | 3 cut(s) | 369, 576, 2307 |
| BstAFI | CTTAAG | 1 cut(s) | 1298 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1652 |
| BstBI | TTCGAA | 1 cut(s) | 1182 |
| BstC8I | GCNNGC | 4 cut(s) | 106, 832, 902, 1006 |
| BstDEI | CTNAG | 2 cut(s) | 7, 1837 |
| BstDSI | CCRYGG | 3 cut(s) | 762, 1052, 1989 |
| BstEII | GGTNACC | 1 cut(s) | 152 |
| BstF5I | GGATG | 7 cut(s) | 27, 331, 747, 1402, 1772, 2047, 2152 |
| BstFNI | CGCG | 1 cut(s) | 1991 |
| BstH2I | RGCGCY | 1 cut(s) | 2244 |
| BstHHI | GCGC | 1 cut(s) | 2243 |
| BstKTI | GATC | 5 cut(s) | 100, 1443, 1692, 1795, 1952 |
| BstMAI | GTCTC | 6 cut(s) | 43, 1165, 1259, 1523, 2100, 2196 |
| BstMBI | GATC | 5 cut(s) | 97, 1440, 1689, 1792, 1949 |
| BstMCI | CGRYCG | 2 cut(s) | 145, 1995 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 101, 144, 645, 836, 906, 1652, 2078 |
| BstNI | CCWGG | 4 cut(s) | 374, 752, 906, 2181 |
| BstNSI | RCATGY | 1 cut(s) | 834 |
| BstPI | GGTNACC | 1 cut(s) | 152 |
| BstSCI | CCNGG | 6 cut(s) | 297, 372, 518, 750, 904, 2179 |
| BstSFI | CTRYAG | 2 cut(s) | 1287, 1644 |
| BstSLI | GKGCMC | 1 cut(s) | 539 |
| BstUI | CGCG | 1 cut(s) | 1991 |
| BstV1I | GCAGC | 2 cut(s) | 68, 1630 |
| BstV2I | GAAGAC | 1 cut(s) | 2226 |
| BstX2I | RGATCY | 2 cut(s) | 1792, 1949 |
| BstYI | RGATCY | 2 cut(s) | 1792, 1949 |
| BstZ17I | GTATAC | 1 cut(s) | 1373 |
| BstZI | CGGCCG | 1 cut(s) | 1992 |
| Bsu15I | ATCGAT | 1 cut(s) | 1688 |
| BsuRI | GGCC | 7 cut(s) | 5, 648, 705, 1221, 1994, 2116, 2171 |
| BsuTUI | ATCGAT | 1 cut(s) | 1688 |
| BtgI | CCRYGG | 3 cut(s) | 762, 1052, 1989 |
| BtsCI | GGATG | 7 cut(s) | 27, 331, 747, 1402, 1772, 2047, 2152 |
| BtsIMutI | CAGTG | 3 cut(s) | 277, 570, 902 |
| BveI | ACCTGC | 1 cut(s) | 1009 |
| Cac8I | GCNNGC | 4 cut(s) | 106, 832, 902, 1006 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1652 |
| CciI | TCATGA | 1 cut(s) | 339 |
| CfoI | GCGC | 1 cut(s) | 2243 |
| Cfr13I | GGNCC | 2 cut(s) | 1049, 1220 |
| Cfr42I | CCGCGG | 1 cut(s) | 1992 |
| ClaI | ATCGAT | 1 cut(s) | 1688 |
| CseI | GACGC | 2 cut(s) | 377, 565 |
| Csp6I | GTAC | 4 cut(s) | 268, 621, 1201, 1834 |
| CspCI | CAANNNNNGTGG | 4 cut(s) | 1602, 1637, 2242, 2277 |
| CviQI | GTAC | 4 cut(s) | 268, 621, 1201, 1834 |
| DdeI | CTNAG | 2 cut(s) | 7, 1837 |
| DpnI | GATC | 5 cut(s) | 99, 1442, 1691, 1794, 1951 |
| DpnII | GATC | 5 cut(s) | 97, 1440, 1689, 1792, 1949 |
| DraI | TTTAAA | 1 cut(s) | 733 |
| EaeI | YGGCCR | 3 cut(s) | 646, 1992, 2169 |
| EagI | CGGCCG | 1 cut(s) | 1992 |
| Eam1104I | CTCTTC | 3 cut(s) | 713, 1953, 2206 |
| EarI | CTCTTC | 3 cut(s) | 713, 1953, 2206 |
| EclXI | CGGCCG | 1 cut(s) | 1992 |
| Eco130I | CCWWGG | 2 cut(s) | 762, 1052 |
| Eco147I | AGGCCT | 1 cut(s) | 2116 |
| Eco47I | GGWCC | 1 cut(s) | 1049 |
| Eco47III | AGCGCT | 1 cut(s) | 2242 |
| Eco52I | CGGCCG | 1 cut(s) | 1992 |
| Eco88I | CYCGRG | 1 cut(s) | 955 |
| Eco91I | GGTNACC | 1 cut(s) | 152 |
| EcoO65I | GGTNACC | 1 cut(s) | 152 |
| EcoRII | CCWGG | 4 cut(s) | 372, 750, 904, 2179 |
| EcoT14I | CCWWGG | 2 cut(s) | 762, 1052 |
| EcoT22I | ATGCAT | 1 cut(s) | 832 |
| ErhI | CCWWGG | 2 cut(s) | 762, 1052 |
| Esp3I | CGTCTC | 2 cut(s) | 1165, 2100 |
| FaqI | GGGAC | 3 cut(s) | 584, 791, 1837 |
| FauI | CCCGC | 1 cut(s) | 609 |
| FbaI | TGATCA | 1 cut(s) | 97 |
| FblI | GTMKAC | 2 cut(s) | 1362, 1372 |
| Fnu4HI | GCNGC | 5 cut(s) | 57, 877, 1219, 1644, 1992 |
| FokI | GGATG | 7 cut(s) | 34, 338, 734, 1409, 1759, 2054, 2159 |
| Fsp4HI | GCNGC | 5 cut(s) | 57, 877, 1219, 1644, 1992 |
| FspBI | CTAG | 3 cut(s) | 726, 1232, 1733 |
| GlaI | GCGC | 1 cut(s) | 2242 |
| GluI | GCNGC | 5 cut(s) | 57, 877, 1219, 1644, 1992 |
| GsuI | CTGGAG | 2 cut(s) | 292, 418 |
| HaeII | RGCGCY | 1 cut(s) | 2244 |
| HaeIII | GGCC | 7 cut(s) | 5, 648, 705, 1221, 1994, 2116, 2171 |
| HapII | CCGG | 3 cut(s) | 209, 298, 519 |
| HgaI | GACGC | 2 cut(s) | 377, 565 |
| HhaI | GCGC | 1 cut(s) | 2243 |
| Hin1I | GRCGYC | 3 cut(s) | 369, 576, 2307 |
| Hin6I | GCGC | 1 cut(s) | 2241 |
| HinP1I | GCGC | 1 cut(s) | 2241 |
| HindIII | AAGCTT | 3 cut(s) | 1120, 1334, 2095 |
| HinfI | GANTC | 6 cut(s) | 274, 563, 1164, 1256, 2011, 2228 |
| HpaII | CCGG | 3 cut(s) | 209, 298, 519 |
| Hpy166II | GTNNAC | 7 cut(s) | 249, 537, 1245, 1363, 1373, 1388, 1746 |
| Hpy188I | TCNGA | 6 cut(s) | 322, 392, 410, 1494, 1762, 1954 |
| Hpy188III | TCNNGA | 8 cut(s) | 340, 1039, 1168, 1262, 1444, 1787, 2021, 2225 |
| Hpy8I | GTNNAC | 7 cut(s) | 249, 537, 1245, 1363, 1373, 1388, 1746 |
| Hpy99I | CGWCG | 3 cut(s) | 371, 581, 2309 |
| HpyAV | CCTTC | 8 cut(s) | 411, 908, 1297, 1337, 2032, 2127, 2267, 2332 |
| HpyCH4III | ACNGT | 3 cut(s) | 204, 620, 625 |
| HpyCH4IV | ACGT | 4 cut(s) | 789, 986, 1884, 2307 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 101, 144, 645, 836, 906, 1652, 2078 |
| HpyF3I | CTNAG | 2 cut(s) | 7, 1837 |
| HpySE526I | ACGT | 4 cut(s) | 789, 986, 1884, 2307 |
| Hsp92I | GRCGYC | 3 cut(s) | 369, 576, 2307 |
| HspAI | GCGC | 1 cut(s) | 2241 |
| Ksp22I | TGATCA | 1 cut(s) | 97 |
| KspI | CCGCGG | 1 cut(s) | 1992 |
| Kzo9I | GATC | 5 cut(s) | 97, 1440, 1689, 1792, 1949 |
| LmnI | GCTCC | 4 cut(s) | 411, 663, 754, 1769 |
| Lsp1109I | GCAGC | 2 cut(s) | 68, 1630 |
| LweI | GCATC | 3 cut(s) | 189, 240, 1781 |
| MaeI | CTAG | 3 cut(s) | 726, 1232, 1733 |
| MaeII | ACGT | 4 cut(s) | 789, 986, 1884, 2307 |
| MalI | GATC | 5 cut(s) | 99, 1442, 1691, 1794, 1951 |
| MboI | GATC | 5 cut(s) | 97, 1440, 1689, 1792, 1949 |
| MboII | GAAGA | 8 cut(s) | 700, 1648, 1802, 1844, 1970, 2193, 2226, 2279 |
| MflI | RGATCY | 2 cut(s) | 1792, 1949 |
| MhlI | GDGCHC | 3 cut(s) | 539, 956, 1587 |
| MlsI | TGGCCA | 1 cut(s) | 2171 |
| MluNI | TGGCCA | 1 cut(s) | 2171 |
| MlyI | GAGTC | 3 cut(s) | 283, 572, 2237 |
| MmeI | TCCRAC | 2 cut(s) | 1602, 1740 |
| Mox20I | TGGCCA | 1 cut(s) | 2171 |
| Mph1103I | ATGCAT | 1 cut(s) | 832 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1851 |
| MscI | TGGCCA | 1 cut(s) | 2171 |
| MslI | CAYNNNNRTG | 3 cut(s) | 1057, 1326, 1707 |
| Msp20I | TGGCCA | 1 cut(s) | 2171 |
| MspA1I | CMGCKG | 3 cut(s) | 95, 147, 1991 |
| MspCI | CTTAAG | 1 cut(s) | 1298 |
| MspI | CCGG | 3 cut(s) | 209, 298, 519 |
| MspR9I | CCNGG | 6 cut(s) | 299, 374, 520, 752, 906, 2181 |
| MvaI | CCWGG | 4 cut(s) | 374, 752, 906, 2181 |
| MvnI | CGCG | 1 cut(s) | 1991 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 101, 144, 645, 836, 906, 1652, 2078 |
| NciI | CCSGG | 2 cut(s) | 299, 520 |
| NcoI | CCATGG | 2 cut(s) | 762, 1052 |
| NdeII | GATC | 5 cut(s) | 97, 1440, 1689, 1792, 1949 |
| NlaIV | GGNNCC | 1 cut(s) | 1785 |
| NmuCI | GTSAC | 8 cut(s) | 152, 185, 239, 275, 437, 467, 1448, 1880 |
| NsiI | ATGCAT | 1 cut(s) | 832 |
| NspI | RCATGY | 1 cut(s) | 834 |
| NspV | TTCGAA | 1 cut(s) | 1182 |
| PaeI | GCATGC | 1 cut(s) | 834 |
| PagI | TCATGA | 1 cut(s) | 339 |
| PaqCI | CACCTGC | 1 cut(s) | 1009 |
| PasI | CCCWGGG | 1 cut(s) | 373 |
| PceI | AGGCCT | 1 cut(s) | 2116 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 493, 795 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1851 |
| PfeI | GAWTC | 3 cut(s) | 1164, 1256, 2011 |
| PfoI | TCCNGGA | 1 cut(s) | 750 |
| PkrI | GCNGC | 5 cut(s) | 58, 878, 1220, 1645, 1993 |
| PleI | GAGTC | 3 cut(s) | 282, 571, 2236 |
| PpsI | GAGTC | 3 cut(s) | 282, 571, 2236 |
| Psp6I | CCWGG | 4 cut(s) | 372, 750, 904, 2179 |
| PspEI | GGTNACC | 1 cut(s) | 152 |
| PspGI | CCWGG | 4 cut(s) | 372, 750, 904, 2179 |
| PspN4I | GGNNCC | 1 cut(s) | 1785 |
| PspPI | GGNCC | 2 cut(s) | 1049, 1220 |
| PstI | CTGCAG | 2 cut(s) | 1291, 1648 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1652 |
| PsuI | RGATCY | 2 cut(s) | 1792, 1949 |
| PvuII | CAGCTG | 1 cut(s) | 95 |
| RsaI | GTAC | 4 cut(s) | 269, 622, 1202, 1835 |
| RsaNI | GTAC | 4 cut(s) | 268, 621, 1201, 1834 |
| RseI | CAYNNNNRTG | 3 cut(s) | 1057, 1326, 1707 |
| SacII | CCGCGG | 1 cut(s) | 1992 |
| SatI | GCNGC | 5 cut(s) | 57, 877, 1219, 1644, 1992 |
| Sau3AI | GATC | 5 cut(s) | 97, 1440, 1689, 1792, 1949 |
| Sau96I | GGNCC | 2 cut(s) | 1049, 1220 |
| ScaI | AGTACT | 1 cut(s) | 1202 |
| SchI | GAGTC | 3 cut(s) | 283, 572, 2237 |
| ScrFI | CCNGG | 6 cut(s) | 299, 374, 520, 752, 906, 2181 |
| SduI | GDGCHC | 3 cut(s) | 539, 956, 1587 |
| SfaNI | GCATC | 3 cut(s) | 189, 240, 1781 |
| SfcI | CTRYAG | 2 cut(s) | 1287, 1644 |
| Sfr303I | CCGCGG | 1 cut(s) | 1992 |
| SfuI | TTCGAA | 1 cut(s) | 1182 |
| SgrBI | CCGCGG | 1 cut(s) | 1992 |
| SinI | GGWCC | 1 cut(s) | 1049 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 1057, 1326, 1707 |
| SmlI | CTYRAG | 6 cut(s) | 120, 594, 1262, 1298, 1391, 2225 |
| SmoI | CTYRAG | 6 cut(s) | 120, 594, 1262, 1298, 1391, 2225 |
| SpeI | ACTAGT | 1 cut(s) | 725 |
| SphI | GCATGC | 1 cut(s) | 834 |
| SseBI | AGGCCT | 1 cut(s) | 2116 |
| SsiI | CCGC | 8 cut(s) | 116, 145, 602, 876, 1218, 1749, 1989, 1991 |
| SspI | AATATT | 4 cut(s) | 316, 1432, 2154, 2293 |
| SspMI | CTAG | 3 cut(s) | 726, 1232, 1733 |
| StuI | AGGCCT | 1 cut(s) | 2116 |
| StyD4I | CCNGG | 6 cut(s) | 297, 372, 518, 750, 904, 2179 |
| StyI | CCWWGG | 2 cut(s) | 762, 1052 |
| TaaI | ACNGT | 3 cut(s) | 204, 620, 625 |
| TaiI | ACGT | 4 cut(s) | 792, 989, 1887, 2310 |
| TaqI | TCGA | 9 cut(s) | 382, 487, 496, 555, 1182, 1443, 1630, 1682, 1688 |
| TatI | WGTACW | 1 cut(s) | 1200 |
| TauI | GCSGC | 3 cut(s) | 879, 1221, 1994 |
| TfiI | GAWTC | 3 cut(s) | 1164, 1256, 2011 |
| TscAI | CASTG | 3 cut(s) | 284, 577, 902 |
| TseFI | GTSAC | 8 cut(s) | 152, 185, 239, 275, 437, 467, 1448, 1880 |
| TseI | GCWGC | 2 cut(s) | 56, 1643 |
| Tsp45I | GTSAC | 8 cut(s) | 152, 185, 239, 275, 437, 467, 1448, 1880 |
| TspGWI | ACGGA | 1 cut(s) | 676 |
| TspRI | CASTG | 3 cut(s) | 284, 577, 902 |
| Vha464I | CTTAAG | 1 cut(s) | 1298 |
| VneI | GTGCAC | 1 cut(s) | 535 |
| VpaK11BI | GGWCC | 1 cut(s) | 1049 |
| XapI | RAATTY | 5 cut(s) | 865, 994, 1098, 1143, 2047 |
| XceI | RCATGY | 1 cut(s) | 834 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 267, 902 |
| XmiI | GTMKAC | 2 cut(s) | 1362, 1372 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1851 |
| XspI | CTAG | 3 cut(s) | 726, 1232, 1733 |
| ZraI | GACGTC | 1 cut(s) | 2308 |
| ZrmI | AGTACT | 1 cut(s) | 1202 |
| Zsp2I | ATGCAT | 1 cut(s) | 832 |