RchiOBHm_Chr6g0259291

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000066
Physical Location & Seq
Reverse (-)
14532776 .. 14535769
2994 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ23248

Sequence Viewer

Length: 2352 bp
ATGGCCTTAGTAGATGTGGGGATGTTTGTTATGCAAGTCTCATTGGCAATAGTAGCAGCTTCAATAACAACCACCAGCACCCAAGTAGCAGCTGATCAAGCCAGCCTCTTACAGCCTCAAGCCAAGCCTAACTGCCCCGACCGTTGTGGTGACCTCACAATCCCATACCCATTTGGCATCGGTGACGGCTGTTACCGACAGTTGCCGGAACAAAATCACCAATTCAGCATCACTTGTGACCAGTCCACCAAACCTCCATCAGCGTACTGGACAAGTGCTAAACGCTGGACAAGTGCTAAAGTCCGGGTTACAAACTTCAACATTTCAGAGGGTGAGTTGCAAATTATGAGTTACATAGCGAAAGATTGTTATGACGCCCAGGGTAGTCGAACATACCGAACCACCCCTTTGCTCCGAGTTGATCCTCCTTTCACCATCTCCAGTCGCAAAAACAAGTTCTTTGCCGTTGGTTGTGACACTTACGCAATTTTTCGAGGTTTTCGAGGGGAAGAAGAGTTGGTAACCGGGTGCATGTCTGTGTGCACCACCCTTGGCGGTGTCGATTGGGAGTCTTGCACTGGCGTCGGCTGTTGCCAAACTCAAGTCCCGCATGGACTGAAAAACCGCACCGTGAGATTGAGTAGCTATAACGGCCACAAGGAGATATGGAGCTTTAACCCTTGTAGTTACGCTTTTATTGTGCAAGATGGCCTCTTCACATTTAATACTACGAGTTTTGAACTACTGAACAACATCCAGGAGCTTCCCATGGTACTTAACTGGGACATGGGAAACGTGCCATGCGAGGTAGCTGGAAAGAGAAGTATGGATTATGCATGCAAGGCTAATAGCAAGTGTGCTAATCGGAGAAATTTCACCGGCGGCAATGGTTACTATTGCCAGTGCTTGCCTGGCTACGAAGGAAACCCATATCTCGGTTGCCAAGATATTGACGAGTGCTCGGCTGAAACTAACCCCTGCGAGAATGGAACATGCCTAAATTCACCTGCTGGCAATTACTCTTGTAAATGTCATAAAGGGTTCCGGAGTGATGGTCCATGGAAGTGCGTTCCCTCCCCCAAAGTTAACAACACTTCCTTGAAAATTTTATTGGGTATCAGTATAAGCGTCTTTGTATTGATGACTGGAATTTTTGGGATATATTGCGGATTCAAGAGGCGACAATTCGAAAGACTTAAAGCAAAGTACTTCGATGCAAATGGCGGCCCAAAGTTGCTAGAAAAATTGTCCACTCAAAAAGAATCTCTTGAGACAAAAGCCCAAATCTTTACTGCAGAAGTACTTAAGAAGGCAACAAACAGTTACCATGAAAGTGAGAAGCTTGGTGAAGGAGGCTATGGAACAGTCTACAAAGGTATACTCAAGGATGAAAGAGTGGTTGCAATAAAGAAGTCCAAAAATACTGCCCAGATCGAGAGTGACCAGTTTGTTAATGAGGTGGTTGTTCTCTCTCAAATCAATCATAAAAATGTGGTGAGGCTTATAGGTTGTTGTTTGGAGACACAAATGCCTTTGTTAGTCTATGAGTTTGTTGGCAACGGCACTCTTTATGAGCACATTCATAGAATAAATGGGAGAGAACCACTTTCGTGGGCGTTTCGATTGAAGATAGCCGCAGAAACTGCAAGTGCACTCTCATACTTACACCACTCGACATCGATCCCAATCATTCACCGAGATGTAAAAGCAACCAATATACTACTAGATGAGAAGTTCACCGCAAAAGTGTCGGACTTTGGAGCTTCCCGTTTGGTTCCCGAAGATCCAAATCAACTTCCAACTATAGTGCAAGGGACAATGGGGTACTTAGACCCTGAATATCTTCAATCAAACATACTCACAGACAAGAGTGACGTTTATAGCTTTGGAGTTGTCCTTGTAGAGCTACTCACAAGCAAAAAGGTGGTTTCATTTGATAGATCCGAGGAAGAGAGAAACCTCGCAAATGTCTTTGTTTCCGCGGCCAAAAAGGGTATGTTGAATCCAATTCTTGATGATGAAATAGTGAAGGATGGAAATTTGGAGATCATTGAAAAAGTGGCCAATCTCGCCAAAAATTGTTTAAGCTTAAAAGGAGACGACAGGCCTTCCATGAAAGATGTAGAGAGGGAACTGGATGGAATATTATTGCAAACTCTGGCCATAGAACCAGGGGTAAAGTCTCATTCCTCTGCCAAAGAGATAGACCACTCTCTTGAGTCGTCTTCAAGCGCTTTCGTTGTCAAGGTTGGTGGTGAAGATGAAGGTGATAACACCACCAATGTTACAAGTTACGACGTCAGCATGGAAAATCAAGCCCAAATGTTGAAGCCTTTTGATGATGGTCGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK cEGF

Protein Analysis

783

Amino Acids

86.53

Weight (kDa)

6.0

Isoelectric Point (pI)

35.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 45 - 115 2.2e-08 Wall-associated receptor kinase galacturonan-binding
EGF_CA PF07645 316 - 352 9e-06 Calcium-binding EGF domain
Pkinase PF00069 442 - 708 4.3e-49 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 445 - 712 2.8e-48 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21210 AT1G21230 AT1G21240 AT1G21240 AT1G21245 AT1G21250 AT1G21270 AT1G22720 AT3G25490
fragaria_vesca FvH4_2g35051 FvH4_2g35051 FvH4_2g35060 FvH4_3g10281 FvH4_3g10285 FvH4_3g10286 FvH4_3g10311 FvH4_3g10311 FvH4_3g10320 FvH4_3g10332 FvH4_3g10340 FvH4_3g10350 FvH4_3g10360 FvH4_3g10390 FvH4_3g10412 FvH4_3g10413 FvH4_3g10461 FvH4_3g10462 FvH4_3g10470 FvH4_3g10510 FvH4_3g10520 FvH4_3g10530 FvH4_3g10550 FvH4_3g10560 FvH4_3g10611 FvH4_3g11730 FvH4_6g53140 FvH4_6g53142 FvH4_7g31880 FvH4_7g31910 FvH4_7g31920
malus_domestica MD05G1269900.v1.1 MD05G1270000.v1.1 MD05G1270200.v1.1 MD05G1270400.v1.1 MD05G1270500.v1.1 MD05G1270600.v1.1 MD05G1270800.v1.1 MD05G1270900.v1.1 MD07G1183800.v1.1 MD08G1107600.v1.1 MD10G1248500.v1.1 MD10G1249200.v1.1 MD10G1249500.v1.1 MD10G1250000.v1.1 MD10G1250500.v1.1 MD10G1250900.v1.1 MD10G1251200.v1.1 MD10G1251400.v1.1 MD13G1004600.v1.1 MD15G1088400.v1.1
prunus_persica Prupe.1G442400_v2.0.a1 Prupe.1G442500_v2.0.a1 Prupe.2G223400_v2.0.a1 Prupe.4G091900_v2.0.a1 Prupe.4G093200_v2.0.a1 Prupe.4G093300_v2.0.a1 Prupe.4G093400_v2.0.a1 Prupe.4G093500_v2.0.a1 Prupe.4G094100_v2.0.a1 Prupe.4G094200_v2.0.a1 Prupe.4G094300_v2.0.a1 Prupe.6G358300_v2.0.a1
pyrus_communis pycom08g08990 pycom10g20660 pycom10g20670 pycom10g20680 pycom10g20710 pycom10g20740 pycom13g00400 pycom15g08290
rosa_chinensis RchiOBHm_Chr1g0326861 RchiOBHm_Chr1g0326871 RchiOBHm_Chr1g0331391 RchiOBHm_Chr1g0336911 RchiOBHm_Chr1g0364001 RchiOBHm_Chr2g0174541 RchiOBHm_Chr2g0175041 RchiOBHm_Chr2g0175101 RchiOBHm_Chr2g0175121 RchiOBHm_Chr3g0486331 RchiOBHm_Chr5g0015581 RchiOBHm_Chr5g0016551 RchiOBHm_Chr5g0016561 RchiOBHm_Chr5g0016581 RchiOBHm_Chr5g0016601 RchiOBHm_Chr5g0016611 RchiOBHm_Chr5g0016641 RchiOBHm_Chr5g0016661 RchiOBHm_Chr5g0016691 RchiOBHm_Chr5g0016701 RchiOBHm_Chr5g0016731 RchiOBHm_Chr5g0016741 RchiOBHm_Chr5g0016811 RchiOBHm_Chr5g0016841 RchiOBHm_Chr5g0016871 RchiOBHm_Chr5g0016881 RchiOBHm_Chr5g0016931 RchiOBHm_Chr5g0017021 RchiOBHm_Chr5g0017041 RchiOBHm_Chr5g0017071 RchiOBHm_Chr5g0017091 RchiOBHm_Chr5g0017121 RchiOBHm_Chr5g0017141 RchiOBHm_Chr5g0017291 RchiOBHm_Chr5g0017321 RchiOBHm_Chr5g0017401 RchiOBHm_Chr5g0019001 RchiOBHm_Chr5g0019071 RchiOBHm_Chr5g0028941 RchiOBHm_Chr5g0052141 RchiOBHm_Chr5g0054721 RchiOBHm_Chr5g0054801 RchiOBHm_Chr5g0054931 RchiOBHm_Chr5g0054941 RchiOBHm_Chr6g0259291 RchiOBHm_Chr6g0292991 RchiOBHm_Chr6g0306911
rosa_laevigata RLG00000009465 RLG00000009468 RLG00000010755 RLG00000011976 RLG00000014541 RLG00000014542 RLG00000015169 RLG00000022308 RLG00000027529 RLG00000029324 RLG00000030244 RLG00000030245 RLG00000032210 RLG00000032211 RLG00000032257 RLG00000032259 RLG00000032260 RLG00000032261 RLG00000032262 RLG00000032268 RLG00000032269 RLG00000032272 RLG00000032282 RLG00000032283 RLG00000032308 RLG00000032413 RLG00000033129 RLG00000034945
rosa_multiflora Rmu_co8241929.1_g000001 Rmu_co8269227.1_g000001 Rmu_co8461259.1_g000001 Rmu_co8495385.1_g000001 Rmu_sc0000028.1_g000003 Rmu_sc0000083.1_g000009 Rmu_sc0000083.1_g000010 Rmu_sc0000083.1_g000031 Rmu_sc0000083.1_g000043 Rmu_sc0000083.1_g000046 Rmu_sc0000083.1_g000047 Rmu_sc0000563.1_g000024 Rmu_sc0000842.1_g000012 Rmu_sc0001235.1_g000001 Rmu_sc0001235.1_g000005 Rmu_sc0001235.1_g000009 Rmu_sc0001235.1_g000015 Rmu_sc0001235.1_g000016 Rmu_sc0001235.1_g000017 Rmu_sc0001473.1_g000036 Rmu_sc0001607.1_g000016 Rmu_sc0001788.1_g000006 Rmu_sc0002018.1_g000008 Rmu_sc0002028.1_g000001 Rmu_sc0002680.1_g000011 Rmu_sc0002798.1_g000023 Rmu_sc0002886.1_g000023 Rmu_sc0002989.1_g000012 Rmu_sc0003064.1_g000009 Rmu_sc0003382.1_g000006 Rmu_sc0003630.1_g000002 Rmu_sc0003630.1_g000047 Rmu_sc0003630.1_g000066 Rmu_sc0003630.1_g000067 Rmu_sc0003649.1_g000010 Rmu_sc0003688.1_g000005 Rmu_sc0003920.1_g000007 Rmu_sc0004765.1_g000007 Rmu_sc0004765.1_g000008 Rmu_sc0004920.1_g000011 Rmu_sc0005223.1_g000008 Rmu_sc0005223.1_g000010 Rmu_sc0005223.1_g000015 Rmu_sc0005223.1_g000016 Rmu_sc0005319.1_g000014 Rmu_sc0005319.1_g000015 Rmu_sc0005319.1_g000018 Rmu_sc0005319.1_g000019 Rmu_sc0005319.1_g000025 Rmu_sc0005418.1_g000007 Rmu_sc0005418.1_g000012 Rmu_sc0006106.1_g000011 Rmu_sc0006914.1_g000012 Rmu_sc0007023.1_g000001 Rmu_sc0007662.1_g000001 Rmu_sc0007920.1_g000002 Rmu_sc0007920.1_g000013 Rmu_sc0008053.1_g000016 Rmu_sc0008053.1_g000017 Rmu_sc0008053.1_g000018 Rmu_sc0008053.1_g000023 Rmu_sc0008053.1_g000050 Rmu_sc0009042.1_g000007 Rmu_sc0009042.1_g000008 Rmu_sc0010642.1_g000008 Rmu_sc0011109.1_g000002 Rmu_sc0011314.1_g000006 Rmu_sc0011974.1_g000006 Rmu_sc0012179.1_g000009 Rmu_sc0015169.1_g000001 Rmu_sc0016368.1_g000002 Rmu_sc0022659.1_g000001 Rmu_sc0027371.1_g000001 Rmu_sc0028213.1_g000001 Rmu_sc0034526.1_g000001 Rmu_sc0039366.1_g000002 Rmu_ssc0000050.1_g000084 Rmu_ssc0000050.1_g000088 Rmu_ssc0000167.1_g000017
rosa_roxburghii Rroxscaffold_1G00011340 Rroxscaffold_1G00025710 Rroxscaffold_1G00025870 Rroxscaffold_1G00026070 Rroxscaffold_1G00026170 Rroxscaffold_1G00026200 Rroxscaffold_1G00026270 Rroxscaffold_1G00050950 Rroxscaffold_1G00058900 Rroxscaffold_1G00060040 Rroxscaffold_1G00060060 Rroxscaffold_1G00060070 Rroxscaffold_1G00060080 Rroxscaffold_1G00060110 Rroxscaffold_1G00060120 Rroxscaffold_1G00060140 Rroxscaffold_1G00060170 Rroxscaffold_1G00060210 Rroxscaffold_1G00060220 Rroxscaffold_1G00060240 Rroxscaffold_1G00060250 Rroxscaffold_1G00060260 Rroxscaffold_1G00060300 Rroxscaffold_1G00060320 Rroxscaffold_1G00060330 Rroxscaffold_1G00060900 Rroxscaffold_1G00060920 Rroxscaffold_1G00073980 Rroxscaffold_2G00077560 Rroxscaffold_2G00077580 Rroxscaffold_2G00146990 Rroxscaffold_3G00236200 Rroxscaffold_3G00236210 Rroxscaffold_3G00236220 Rroxscaffold_4G00292760 Rroxscaffold_4G00315200 Rroxscaffold_4G00315240 Rroxscaffold_4G00326240 Rroxscaffold_4G00328980 Rroxscaffold_5G00341890 Rroxscaffold_7G00161350 Rroxscaffold_7G00174490 Rroxscaffold_7G00206550
rosa_rugosa Rorug01G0093000 Rorug01G0093100 Rorug01G0128800 Rorug01G0312000 Rorug01G0312000 Rorug02G0581100 Rorug03G0224600 Rorug05G0028500 Rorug05G0032700 Rorug05G0032800 Rorug05G0032900 Rorug05G0032900 Rorug05G0033000 Rorug05G0033100 Rorug05G0033300 Rorug05G0033400 Rorug05G0033900 Rorug05G0034900 Rorug05G0035000 Rorug05G0046900 Rorug05G0047500 Rorug05G0047500 Rorug05G0111600 Rorug05G0287900 Rorug05G0288700 Rorug05G0586900 Rorug06G0231700 Rorug06G0354300 Rorug06G0354400
rosa_samantha Rh1DG122200 Rh2DG686800 Rh5BG136600 Rh6AG344500 Rh6BG351900 Rh6CG359000 Rh6CG359100 Rh6CG387600 Rh6CG480500 Rh6DG344600 Rh6DG467400
rosa_wichuraiana Rw0G010250 Rw0G014820 Rw0G021400 Rw0G023100 Rw1G009450 Rw1G012100 Rw1G012180 Rw1G028360 Rw2G054260 Rw3G024370 Rw3G024420 Rw3G024470 Rw5G010850 Rw5G011010 Rw5G011020 Rw5G011050 Rw5G011080 Rw5G011120 Rw5G012300 Rw5G018470 Rw5G033830 Rw6G008930 Rw6G030070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1015
AatII GACGTC 1 cut(s) 2301
Acc36I ACCTGC 1 cut(s) 1015
AccI GTMKAC 2 cut(s) 1368, 1378
AccII CGCG 1 cut(s) 1982
AccIII TCCGGA 1 cut(s) 1044
AclWI GGATC 4 cut(s) 416, 1675, 1778, 1935
AcoI YGGCCR 4 cut(s) 652, 1983, 2061, 2160
AcsI RAATTY 5 cut(s) 871, 1000, 1104, 1149, 2038
AcyI GRCGYC 3 cut(s) 375, 582, 2298
AfaI GTAC 5 cut(s) 266, 774, 1208, 1302, 1826
AfeI AGCGCT 1 cut(s) 2233
AfiI CCNNNNNNNGG 1 cut(s) 935
AflII CTTAAG 1 cut(s) 1304
AjnI CCWGG 4 cut(s) 378, 756, 910, 2170
AjuI GAANNNNNNNTTGG 2 cut(s) 1094, 1126
AleI CACNNNNGTG 1 cut(s) 1609
Alw21I GWGCWC 4 cut(s) 545, 962, 1578, 1654
Alw26I GTCTC 5 cut(s) 43, 1265, 1514, 2091, 2187
Alw44I GTGCAC 2 cut(s) 541, 1650
AlwI GGATC 4 cut(s) 416, 1675, 1778, 1935
AlwNI CAGNNNCTG 1 cut(s) 1643
Aor13HI TCCGGA 1 cut(s) 1044
Aor51HI AGCGCT 1 cut(s) 2233
AoxI GGCC 8 cut(s) 3, 652, 709, 1225, 1983, 2061, 2105, 2160
ApaLI GTGCAC 2 cut(s) 541, 1650
ApeKI GCWGC 2 cut(s) 56, 89
ApoI RAATTY 5 cut(s) 871, 1000, 1104, 1149, 2038
Asp700I GAANNNNTTC 1 cut(s) 1842
AspLEI GCGC 1 cut(s) 2234
AspS9I GGNCC 2 cut(s) 1055, 1226
AsuC2I CCSGG 2 cut(s) 305, 526
AsuII TTCGAA 1 cut(s) 1188
AvaII GGWCC 1 cut(s) 1055
BaeGI GKGCMC 2 cut(s) 545, 1654
BalI TGGCCA 2 cut(s) 2063, 2162
BbsI GAAGAC 1 cut(s) 2217
Bbv12I GWGCWC 4 cut(s) 545, 962, 1578, 1654
BbvI GCAGC 2 cut(s) 68, 101
BccI CCATC 7 cut(s) 265, 443, 701, 1046, 2027, 2132, 2336
BceAI ACGGC 4 cut(s) 202, 449, 667, 1576
BcgI CGANNNNNNTGC 4 cut(s) 565, 599, 1731, 1765
BciT130I CCWGG 4 cut(s) 380, 758, 912, 2172
BclI TGATCA 1 cut(s) 94
BcnI CCSGG 2 cut(s) 305, 526
BcoDI GTCTC 5 cut(s) 43, 1265, 1514, 2091, 2187
BfaI CTAG 2 cut(s) 1238, 1724
BfmI CTRYAG 2 cut(s) 1293, 1803
BfoI RGCGCY 1 cut(s) 2235
BfrI CTTAAG 1 cut(s) 1304
BfuAI ACCTGC 1 cut(s) 1015
BisI GCNGC 6 cut(s) 57, 90, 883, 1225, 1635, 1983
BlsI GCNGC 6 cut(s) 58, 91, 884, 1226, 1636, 1984
BmcAI AGTACT 2 cut(s) 1208, 1302
Bme1390I CCNGG 6 cut(s) 305, 380, 526, 758, 912, 2172
Bme18I GGWCC 1 cut(s) 1055
BmgT120I GGNCC 2 cut(s) 1055, 1226
BmiI GGNNCC 2 cut(s) 1043, 1776
BmrFI CCNGG 6 cut(s) 305, 380, 526, 758, 912, 2172
BmrI ACTGGG 1 cut(s) 790
BmsI GCATC 3 cut(s) 186, 237, 1204
BmuI ACTGGG 1 cut(s) 790
BpiI GAAGAC 1 cut(s) 2217
BpmI CTGGAG 1 cut(s) 424
Bpu14I TTCGAA 1 cut(s) 1188
BpuEI CTTGAG 5 cut(s) 102, 585, 1289, 1367, 2237
BpuMI CCSGG 2 cut(s) 305, 526
Bsa29I ATCGAT 1 cut(s) 1679
BsaBI GATNNNNATC 2 cut(s) 1685, 1788
BsaHI GRCGYC 3 cut(s) 375, 582, 2298
BsaJI CCNNGG 8 cut(s) 378, 379, 550, 768, 1058, 1944, 1980, 2171
BsaWI WCCGGW 1 cut(s) 1044
BsaXI ACNNNNNCTCC 4 cut(s) 238, 268, 1039, 1069
Bsc4I CCNNNNNNNGG 1 cut(s) 935
Bse118I RCCGGY 1 cut(s) 878
Bse1I ACTGG 9 cut(s) 241, 272, 441, 583, 785, 901, 1150, 1444, 2139
Bse3DI GCAATG 1 cut(s) 892
Bse8I GATNNNNATC 2 cut(s) 1685, 1788
BseAI TCCGGA 1 cut(s) 1044
BseBI CCWGG 4 cut(s) 380, 758, 912, 2172
BseCI ATCGAT 1 cut(s) 1679
BseDI CCNNGG 8 cut(s) 378, 379, 550, 768, 1058, 1944, 1980, 2171
BseGI GGATG 5 cut(s) 27, 753, 1393, 2038, 2143
BseJI GATNNNNATC 2 cut(s) 1685, 1788
BseLI CCNNNNNNNGG 1 cut(s) 935
BseMI GCAATG 1 cut(s) 892
BseNI ACTGG 9 cut(s) 241, 272, 441, 583, 785, 901, 1150, 1444, 2139
BseSI GKGCMC 2 cut(s) 545, 1654
BseXI GCAGC 2 cut(s) 68, 101
Bsh1236I CGCG 1 cut(s) 1982
Bsh1285I CGRYCG 1 cut(s) 142
BshFI GGCC 8 cut(s) 5, 654, 711, 1227, 1985, 2063, 2107, 2162
BshVI ATCGAT 1 cut(s) 1679
BsiEI CGRYCG 1 cut(s) 142
BsiHKAI GWGCWC 4 cut(s) 545, 962, 1578, 1654
BsiSI CCGG 5 cut(s) 206, 304, 525, 879, 1045
BslFI GGGAC 3 cut(s) 590, 797, 1828
BslI CCNNNNNNNGG 1 cut(s) 935
BsmAI GTCTC 5 cut(s) 43, 1265, 1514, 2091, 2187
BsmBI CGTCTC 1 cut(s) 2091
BsmFI GGGAC 3 cut(s) 590, 797, 1828
BsnI GGCC 8 cut(s) 5, 654, 711, 1227, 1985, 2063, 2107, 2162
Bsp119I TTCGAA 1 cut(s) 1188
Bsp1286I GDGCHC 4 cut(s) 545, 962, 1578, 1654
Bsp13I TCCGGA 1 cut(s) 1044
Bsp143I GATC 7 cut(s) 94, 421, 1431, 1680, 1783, 1940, 2046
Bsp19I CCATGG 2 cut(s) 768, 1058
BspANI GGCC 8 cut(s) 5, 654, 711, 1227, 1985, 2063, 2107, 2162
BspDI ATCGAT 1 cut(s) 1679
BspEI TCCGGA 1 cut(s) 1044
BspFNI CGCG 1 cut(s) 1982
BspLI GGNNCC 2 cut(s) 1043, 1776
BspMAI CTGCAG 1 cut(s) 1297
BspMI ACCTGC 1 cut(s) 1015
BspPI GGATC 4 cut(s) 416, 1675, 1778, 1935
BspT104I TTCGAA 1 cut(s) 1188
BspTI CTTAAG 1 cut(s) 1304
BsrDI GCAATG 1 cut(s) 892
BsrFI RCCGGY 1 cut(s) 878
BsrI ACTGG 9 cut(s) 241, 272, 441, 583, 785, 901, 1150, 1444, 2139
BssAI RCCGGY 1 cut(s) 878
BssECI CCNNGG 8 cut(s) 378, 379, 550, 768, 1058, 1944, 1980, 2171
BssMI GATC 7 cut(s) 94, 421, 1431, 1680, 1783, 1940, 2046
BssNAI GTATAC 1 cut(s) 1379
BssNI GRCGYC 3 cut(s) 375, 582, 2298
BssT1I CCWWGG 3 cut(s) 550, 768, 1058
Bst1107I GTATAC 1 cut(s) 1379
Bst2UI CCWGG 4 cut(s) 380, 758, 912, 2172
Bst4CI ACNGT 5 cut(s) 143, 201, 631, 1322, 1366
Bst6I CTCTTC 3 cut(s) 507, 719, 1944
BstACI GRCGYC 3 cut(s) 375, 582, 2298
BstAFI CTTAAG 1 cut(s) 1304
BstAPI GCANNNNNTGC 1 cut(s) 1643
BstBI TTCGAA 1 cut(s) 1188
BstC8I GCNNGC 4 cut(s) 103, 838, 908, 1012
BstDEI CTNAG 2 cut(s) 7, 1828
BstDSI CCRYGG 3 cut(s) 768, 1058, 1980
BstEII GGTNACC 2 cut(s) 149, 520
BstF5I GGATG 5 cut(s) 27, 753, 1393, 2038, 2143
BstFNI CGCG 1 cut(s) 1982
BstH2I RGCGCY 1 cut(s) 2235
BstHHI GCGC 1 cut(s) 2234
BstKTI GATC 7 cut(s) 97, 424, 1434, 1683, 1786, 1943, 2049
BstMAI GTCTC 5 cut(s) 43, 1265, 1514, 2091, 2187
BstMBI GATC 7 cut(s) 94, 421, 1431, 1680, 1783, 1940, 2046
BstMCI CGRYCG 1 cut(s) 142
BstMWI GCNNNNNNNGC 7 cut(s) 53, 98, 651, 842, 912, 1643, 2069
BstNI CCWGG 4 cut(s) 380, 758, 912, 2172
BstNSI RCATGY 3 cut(s) 535, 840, 996
BstPI GGTNACC 2 cut(s) 149, 520
BstSCI CCNGG 6 cut(s) 303, 378, 524, 756, 910, 2170
BstSFI CTRYAG 2 cut(s) 1293, 1803
BstSLI GKGCMC 2 cut(s) 545, 1654
BstUI CGCG 1 cut(s) 1982
BstV1I GCAGC 2 cut(s) 68, 101
BstV2I GAAGAC 1 cut(s) 2217
BstX2I RGATCY 2 cut(s) 1783, 1940
BstXI CCANNNNNNTGG 1 cut(s) 1611
BstYI RGATCY 2 cut(s) 1783, 1940
BstZ17I GTATAC 1 cut(s) 1379
Bsu15I ATCGAT 1 cut(s) 1679
BsuRI GGCC 8 cut(s) 5, 654, 711, 1227, 1985, 2063, 2107, 2162
BsuTUI ATCGAT 1 cut(s) 1679
BtgI CCRYGG 3 cut(s) 768, 1058, 1980
BtsCI GGATG 5 cut(s) 27, 753, 1393, 2038, 2143
BtsIMutI CAGTG 2 cut(s) 576, 908
BveI ACCTGC 1 cut(s) 1015
Cac8I GCNNGC 4 cut(s) 103, 838, 908, 1012
CaiI CAGNNNCTG 1 cut(s) 1643
CfoI GCGC 1 cut(s) 2234
Cfr10I RCCGGY 1 cut(s) 878
Cfr13I GGNCC 2 cut(s) 1055, 1226
Cfr42I CCGCGG 1 cut(s) 1983
ClaI ATCGAT 1 cut(s) 1679
CseI GACGC 3 cut(s) 383, 571, 1117
Csp6I GTAC 5 cut(s) 265, 773, 1207, 1301, 1825
CspCI CAANNNNNGTGG 2 cut(s) 2233, 2268
CviQI GTAC 5 cut(s) 265, 773, 1207, 1301, 1825
DdeI CTNAG 2 cut(s) 7, 1828
DpnI GATC 7 cut(s) 96, 423, 1433, 1682, 1785, 1942, 2048
DpnII GATC 7 cut(s) 94, 421, 1431, 1680, 1783, 1940, 2046
EaeI YGGCCR 4 cut(s) 652, 1983, 2061, 2160
Eam1104I CTCTTC 3 cut(s) 507, 719, 1944
EarI CTCTTC 3 cut(s) 507, 719, 1944
Eco130I CCWWGG 3 cut(s) 550, 768, 1058
Eco147I AGGCCT 1 cut(s) 2107
Eco47I GGWCC 1 cut(s) 1055
Eco47III AGCGCT 1 cut(s) 2233
Eco91I GGTNACC 2 cut(s) 149, 520
EcoO65I GGTNACC 2 cut(s) 149, 520
EcoRII CCWGG 4 cut(s) 378, 756, 910, 2170
EcoT14I CCWWGG 3 cut(s) 550, 768, 1058
EcoT22I ATGCAT 1 cut(s) 838
ErhI CCWWGG 3 cut(s) 550, 768, 1058
Esp3I CGTCTC 1 cut(s) 2091
FalI AAGNNNNNCTT 2 cut(s) 1251, 1283
FaqI GGGAC 3 cut(s) 590, 797, 1828
FauI CCCGC 1 cut(s) 615
FbaI TGATCA 1 cut(s) 94
FblI GTMKAC 2 cut(s) 1368, 1378
Fnu4HI GCNGC 6 cut(s) 57, 90, 883, 1225, 1635, 1983
FokI GGATG 5 cut(s) 34, 740, 1400, 2045, 2150
Fsp4HI GCNGC 6 cut(s) 57, 90, 883, 1225, 1635, 1983
FspBI CTAG 2 cut(s) 1238, 1724
GlaI GCGC 1 cut(s) 2233
GluI GCNGC 6 cut(s) 57, 90, 883, 1225, 1635, 1983
GsuI CTGGAG 1 cut(s) 424
HaeII RGCGCY 1 cut(s) 2235
HaeIII GGCC 8 cut(s) 5, 654, 711, 1227, 1985, 2063, 2107, 2162
HapII CCGG 5 cut(s) 206, 304, 525, 879, 1045
HgaI GACGC 3 cut(s) 383, 571, 1117
HhaI GCGC 1 cut(s) 2234
Hin1I GRCGYC 3 cut(s) 375, 582, 2298
Hin6I GCGC 1 cut(s) 2232
HinP1I GCGC 1 cut(s) 2232
HincII GTYRAC 1 cut(s) 1087
HindII GTYRAC 1 cut(s) 1087
HindIII AAGCTT 2 cut(s) 1340, 2086
HinfI GANTC 5 cut(s) 569, 1170, 1262, 2002, 2219
HpaI GTTAAC 1 cut(s) 1087
HpaII CCGG 5 cut(s) 206, 304, 525, 879, 1045
Hpy166II GTNNAC 8 cut(s) 246, 543, 1087, 1251, 1369, 1379, 1652, 1737
Hpy188I TCNGA 5 cut(s) 328, 416, 867, 1753, 1945
Hpy188III TCNNGA 7 cut(s) 1045, 1174, 1268, 1435, 1778, 2012, 2216
Hpy8I GTNNAC 8 cut(s) 246, 543, 1087, 1251, 1369, 1379, 1652, 1737
Hpy99I CGWCG 2 cut(s) 587, 2300
HpyAV CCTTC 6 cut(s) 914, 1303, 1343, 2023, 2118, 2258
HpyCH4III ACNGT 5 cut(s) 143, 201, 631, 1322, 1366
HpyCH4IV ACGT 3 cut(s) 795, 1875, 2298
HpyF10VI GCNNNNNNNGC 7 cut(s) 53, 98, 651, 842, 912, 1643, 2069
HpyF3I CTNAG 2 cut(s) 7, 1828
HpySE526I ACGT 3 cut(s) 795, 1875, 2298
Hsp92I GRCGYC 3 cut(s) 375, 582, 2298
HspAI GCGC 1 cut(s) 2232
Kpn2I TCCGGA 1 cut(s) 1044
Ksp22I TGATCA 1 cut(s) 94
KspAI GTTAAC 1 cut(s) 1087
KspI CCGCGG 1 cut(s) 1983
Kzo9I GATC 7 cut(s) 94, 421, 1431, 1680, 1783, 1940, 2046
LmnI GCTCC 4 cut(s) 417, 669, 760, 1760
Lsp1109I GCAGC 2 cut(s) 68, 101
LweI GCATC 3 cut(s) 186, 237, 1204
MaeI CTAG 2 cut(s) 1238, 1724
MaeII ACGT 3 cut(s) 795, 1875, 2298
MalI GATC 7 cut(s) 96, 423, 1433, 1682, 1785, 1942, 2048
MboI GATC 7 cut(s) 94, 421, 1431, 1680, 1783, 1940, 2046
MboII GAAGA 9 cut(s) 521, 524, 706, 1639, 1793, 1835, 1961, 2217, 2270
MflI RGATCY 2 cut(s) 1783, 1940
MhlI GDGCHC 4 cut(s) 545, 962, 1578, 1654
MlsI TGGCCA 2 cut(s) 2063, 2162
MluNI TGGCCA 2 cut(s) 2063, 2162
MlyI GAGTC 2 cut(s) 578, 2228
MmeI TCCRAC 2 cut(s) 1731, 1823
Mox20I TGGCCA 2 cut(s) 2063, 2162
Mph1103I ATGCAT 1 cut(s) 838
MroI TCCGGA 1 cut(s) 1044
MroXI GAANNNNTTC 1 cut(s) 1842
MscI TGGCCA 2 cut(s) 2063, 2162
MseI TTAA 9 cut(s) 675, 723, 777, 1086, 1197, 1305, 1452, 2084, 2090
MslI CAYNNNNRTG 6 cut(s) 536, 1063, 1332, 1488, 1609, 1698
Msp20I TGGCCA 2 cut(s) 2063, 2162
MspA1I CMGCKG 2 cut(s) 92, 1982
MspCI CTTAAG 1 cut(s) 1304
MspI CCGG 5 cut(s) 206, 304, 525, 879, 1045
MspR9I CCNGG 6 cut(s) 305, 380, 526, 758, 912, 2172
MvaI CCWGG 4 cut(s) 380, 758, 912, 2172
MvnI CGCG 1 cut(s) 1982
MwoI GCNNNNNNNGC 7 cut(s) 53, 98, 651, 842, 912, 1643, 2069
NciI CCSGG 2 cut(s) 305, 526
NcoI CCATGG 2 cut(s) 768, 1058
NdeII GATC 7 cut(s) 94, 421, 1431, 1680, 1783, 1940, 2046
NlaIV GGNNCC 2 cut(s) 1043, 1776
NmeAIII GCCGAG 1 cut(s) 941
NmuCI GTSAC 6 cut(s) 149, 182, 236, 473, 1439, 1871
NsiI ATGCAT 1 cut(s) 838
NspI RCATGY 3 cut(s) 535, 840, 996
NspV TTCGAA 1 cut(s) 1188
OliI CACNNNNGTG 1 cut(s) 1609
PaeI GCATGC 1 cut(s) 840
PaqCI CACCTGC 1 cut(s) 1015
PasI CCCWGGG 1 cut(s) 379
PceI AGGCCT 1 cut(s) 2107
PcsI WCGNNNNNNNCGW 3 cut(s) 394, 499, 801
PdmI GAANNNNTTC 1 cut(s) 1842
PfeI GAWTC 3 cut(s) 1170, 1262, 2002
PfoI TCCNGGA 1 cut(s) 756
PkrI GCNGC 6 cut(s) 58, 91, 884, 1226, 1636, 1984
PleI GAGTC 2 cut(s) 577, 2227
PpsI GAGTC 2 cut(s) 577, 2227
Psp6I CCWGG 4 cut(s) 378, 756, 910, 2170
PspEI GGTNACC 2 cut(s) 149, 520
PspGI CCWGG 4 cut(s) 378, 756, 910, 2170
PspN4I GGNNCC 2 cut(s) 1043, 1776
PspPI GGNCC 2 cut(s) 1055, 1226
PstI CTGCAG 1 cut(s) 1297
PstNI CAGNNNCTG 1 cut(s) 1643
PsuI RGATCY 2 cut(s) 1783, 1940
PvuII CAGCTG 1 cut(s) 92
RsaI GTAC 5 cut(s) 266, 774, 1208, 1302, 1826
RsaNI GTAC 5 cut(s) 265, 773, 1207, 1301, 1825
RseI CAYNNNNRTG 6 cut(s) 536, 1063, 1332, 1488, 1609, 1698
SacII CCGCGG 1 cut(s) 1983
SaqAI TTAA 9 cut(s) 675, 723, 777, 1086, 1197, 1305, 1452, 2084, 2090
SatI GCNGC 6 cut(s) 57, 90, 883, 1225, 1635, 1983
Sau3AI GATC 7 cut(s) 94, 421, 1431, 1680, 1783, 1940, 2046
Sau96I GGNCC 2 cut(s) 1055, 1226
ScaI AGTACT 2 cut(s) 1208, 1302
SchI GAGTC 2 cut(s) 578, 2228
ScrFI CCNGG 6 cut(s) 305, 380, 526, 758, 912, 2172
SduI GDGCHC 4 cut(s) 545, 962, 1578, 1654
SfaNI GCATC 3 cut(s) 186, 237, 1204
SfcI CTRYAG 2 cut(s) 1293, 1803
Sfr303I CCGCGG 1 cut(s) 1983
SfuI TTCGAA 1 cut(s) 1188
SgrAI CRCCGGYG 1 cut(s) 878
SgrBI CCGCGG 1 cut(s) 1983
SinI GGWCC 1 cut(s) 1055
SmiMI CAYNNNNRTG 6 cut(s) 536, 1063, 1332, 1488, 1609, 1698
SmlI CTYRAG 6 cut(s) 117, 600, 1268, 1304, 1382, 2216
SmoI CTYRAG 6 cut(s) 117, 600, 1268, 1304, 1382, 2216
SphI GCATGC 1 cut(s) 840
SseBI AGGCCT 1 cut(s) 2107
SspI AATATT 1 cut(s) 2145
SspMI CTAG 2 cut(s) 1238, 1724
StuI AGGCCT 1 cut(s) 2107
StyD4I CCNGG 6 cut(s) 303, 378, 524, 756, 910, 2170
StyI CCWWGG 3 cut(s) 550, 768, 1058
TaaI ACNGT 5 cut(s) 143, 201, 631, 1322, 1366
TaiI ACGT 3 cut(s) 798, 1878, 2301
TatI WGTACW 2 cut(s) 1206, 1300
TauI GCSGC 4 cut(s) 885, 1227, 1637, 1985
TfiI GAWTC 3 cut(s) 1170, 1262, 2002
Tru1I TTAA 9 cut(s) 675, 723, 777, 1086, 1197, 1305, 1452, 2084, 2090
Tru9I TTAA 9 cut(s) 675, 723, 777, 1086, 1197, 1305, 1452, 2084, 2090
TscAI CASTG 2 cut(s) 583, 908
TseFI GTSAC 6 cut(s) 149, 182, 236, 473, 1439, 1871
TseI GCWGC 2 cut(s) 56, 89
Tsp45I GTSAC 6 cut(s) 149, 182, 236, 473, 1439, 1871
TspDTI ATGAA 7 cut(s) 1344, 1404, 1571, 1920, 2034, 2129, 2277
TspRI CASTG 2 cut(s) 583, 908
Vha464I CTTAAG 1 cut(s) 1304
VneI GTGCAC 2 cut(s) 541, 1650
VpaK11BI GGWCC 1 cut(s) 1055
XapI RAATTY 5 cut(s) 871, 1000, 1104, 1149, 2038
XceI RCATGY 3 cut(s) 535, 840, 996
XcmI CCANNNNNNNNNTGG 2 cut(s) 264, 908
XmiI GTMKAC 2 cut(s) 1368, 1378
XmnI GAANNNNTTC 1 cut(s) 1842
XspI CTAG 2 cut(s) 1238, 1724
ZraI GACGTC 1 cut(s) 2299
ZrmI AGTACT 2 cut(s) 1208, 1302
Zsp2I ATGCAT 1 cut(s) 838
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.