Wall-associated receptor kinase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 866 |
| AccB1I | GGYRCC | 1 cut(s) | 1412 |
| AccI | GTMKAC | 1 cut(s) | 1661 |
| AccII | CGCG | 4 cut(s) | 86, 632, 950, 1756 |
| AciI | CCGC | 7 cut(s) | 60, 84, 101, 138, 967, 1283, 1592 |
| AclI | AACGTT | 1 cut(s) | 444 |
| AclWI | GGATC | 1 cut(s) | 1594 |
| AcoI | YGGCCR | 2 cut(s) | 57, 2112 |
| AcsI | RAATTY | 4 cut(s) | 991, 1995, 2170, 2298 |
| AcuI | CTGAAG | 3 cut(s) | 1018, 1533, 1953 |
| AcyI | GRCGYC | 1 cut(s) | 1413 |
| AfaI | GTAC | 4 cut(s) | 259, 1199, 2195, 2272 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 923 |
| AgsI | TTSAA | 9 cut(s) | 615, 683, 722, 1204, 1573, 1615, 1994, 2100, 2303 |
| AhlI | ACTAGT | 2 cut(s) | 1525, 1792 |
| AjiI | CACGTC | 1 cut(s) | 1690 |
| AjnI | CCWGG | 3 cut(s) | 118, 898, 1070 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 407, 439 |
| Alw21I | GWGCWC | 2 cut(s) | 147, 854 |
| Alw26I | GTCTC | 4 cut(s) | 860, 1502, 1680, 2180 |
| AlwI | GGATC | 1 cut(s) | 1594 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 895 |
| Ama87I | CYCGRG | 3 cut(s) | 194, 383, 2180 |
| AoxI | GGCC | 6 cut(s) | 3, 57, 166, 501, 2093, 2112 |
| ApeKI | GCWGC | 7 cut(s) | 34, 80, 372, 388, 663, 745, 969 |
| ApoI | RAATTY | 4 cut(s) | 991, 1995, 2170, 2298 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 1199, 1830 |
| AspA2I | CCTAGG | 1 cut(s) | 169 |
| AspLEI | GCGC | 4 cut(s) | 88, 563, 950, 1415 |
| AspS9I | GGNCC | 1 cut(s) | 1819 |
| AsuC2I | CCSGG | 1 cut(s) | 1002 |
| AsuNHI | GCTAGC | 1 cut(s) | 76 |
| AvaI | CYCGRG | 3 cut(s) | 194, 383, 2180 |
| AvaII | GGWCC | 1 cut(s) | 1819 |
| AvrII | CCTAGG | 1 cut(s) | 169 |
| BaeGI | GKGCMC | 1 cut(s) | 1642 |
| BalI | TGGCCA | 1 cut(s) | 2114 |
| BanI | GGYRCC | 1 cut(s) | 1412 |
| BanII | GRGCYC | 1 cut(s) | 147 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 1506, 1538 |
| BauI | CACGAG | 1 cut(s) | 1012 |
| Bbv12I | GWGCWC | 2 cut(s) | 147, 854 |
| BbvI | GCAGC | 7 cut(s) | 21, 92, 375, 384, 675, 757, 956 |
| BccI | CCATC | 6 cut(s) | 212, 244, 281, 413, 581, 863 |
| BceAI | ACGGC | 3 cut(s) | 637, 1241, 1647 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 364, 398, 770, 804 |
| BciT130I | CCWGG | 3 cut(s) | 120, 900, 1072 |
| BclI | TGATCA | 1 cut(s) | 637 |
| BcnI | CCSGG | 1 cut(s) | 1002 |
| BcoDI | GTCTC | 4 cut(s) | 860, 1502, 1680, 2180 |
| BcuI | ACTAGT | 2 cut(s) | 1525, 1792 |
| BfmI | CTRYAG | 1 cut(s) | 873 |
| BfoI | RGCGCY | 1 cut(s) | 1416 |
| BfuAI | ACCTGC | 1 cut(s) | 866 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 900 |
| BlnI | CCTAGG | 1 cut(s) | 169 |
| BmcAI | AGTACT | 2 cut(s) | 1199, 2195 |
| Bme1390I | CCNGG | 4 cut(s) | 120, 900, 1002, 1072 |
| Bme18I | GGWCC | 1 cut(s) | 1819 |
| BmeT110I | CYCGRG | 3 cut(s) | 194, 383, 2180 |
| BmgBI | CACGTC | 1 cut(s) | 1690 |
| BmgT120I | GGNCC | 1 cut(s) | 1819 |
| BmiI | GGNNCC | 5 cut(s) | 914, 1255, 1342, 1414, 1821 |
| BmrFI | CCNGG | 4 cut(s) | 120, 900, 1002, 1072 |
| BmrI | ACTGGG | 1 cut(s) | 652 |
| BmsI | GCATC | 8 cut(s) | 113, 555, 656, 1068, 1631, 1934, 2158, 2314 |
| BmtI | GCTAGC | 1 cut(s) | 80 |
| BmuI | ACTGGG | 1 cut(s) | 652 |
| BpuEI | CTTGAG | 2 cut(s) | 93, 1313 |
| BpuMI | CCSGG | 1 cut(s) | 1002 |
| Bsa29I | ATCGAT | 1 cut(s) | 939 |
| BsaAI | YACGTR | 1 cut(s) | 975 |
| BsaHI | GRCGYC | 1 cut(s) | 1413 |
| BsaJI | CCNNGG | 8 cut(s) | 169, 433, 922, 1257, 1546, 2115, 2153, 2181 |
| BsaWI | WCCGGW | 3 cut(s) | 488, 751, 846 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 483, 513, 1242, 1272, 2177, 2207 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 923 |
| Bse1I | ACTGG | 2 cut(s) | 647, 889 |
| Bse3DI | GCAATG | 1 cut(s) | 71 |
| BseBI | CCWGG | 3 cut(s) | 120, 900, 1072 |
| BseCI | ATCGAT | 1 cut(s) | 939 |
| BseDI | CCNNGG | 8 cut(s) | 169, 433, 922, 1257, 1546, 2115, 2153, 2181 |
| BseGI | GGATG | 5 cut(s) | 128, 478, 573, 1059, 2149 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 923 |
| BseMI | GCAATG | 1 cut(s) | 71 |
| BseMII | CTCAG | 1 cut(s) | 2181 |
| BseNI | ACTGG | 2 cut(s) | 647, 889 |
| BseRI | GAGGAG | 4 cut(s) | 96, 365, 1266, 2198 |
| BseSI | GKGCMC | 1 cut(s) | 1642 |
| BseXI | GCAGC | 7 cut(s) | 21, 92, 375, 384, 675, 757, 956 |
| BseYI | CCCAGC | 1 cut(s) | 2200 |
| BsgI | GTGCAG | 3 cut(s) | 682, 810, 955 |
| Bsh1236I | CGCG | 4 cut(s) | 86, 632, 950, 1756 |
| Bsh1285I | CGRYCG | 2 cut(s) | 847, 967 |
| BshFI | GGCC | 6 cut(s) | 5, 59, 168, 503, 2095, 2114 |
| BshNI | GGYRCC | 1 cut(s) | 1412 |
| BshVI | ATCGAT | 1 cut(s) | 939 |
| BsiEI | CGRYCG | 2 cut(s) | 847, 967 |
| BsiHKAI | GWGCWC | 2 cut(s) | 147, 854 |
| BsiHKCI | CYCGRG | 3 cut(s) | 194, 383, 2180 |
| BsiSI | CCGG | 4 cut(s) | 489, 752, 847, 1002 |
| BslFI | GGGAC | 2 cut(s) | 791, 1816 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 923 |
| BsmAI | GTCTC | 4 cut(s) | 860, 1502, 1680, 2180 |
| BsmBI | CGTCTC | 2 cut(s) | 860, 1680 |
| BsmFI | GGGAC | 2 cut(s) | 791, 1816 |
| BsmI | GAATGC | 1 cut(s) | 2136 |
| BsnI | GGCC | 6 cut(s) | 5, 59, 168, 503, 2095, 2114 |
| BsoBI | CYCGRG | 3 cut(s) | 194, 383, 2180 |
| Bsp1286I | GDGCHC | 3 cut(s) | 147, 854, 1642 |
| Bsp143I | GATC | 5 cut(s) | 214, 637, 1045, 1586, 1771 |
| Bsp19I | CCATGG | 3 cut(s) | 1546, 2115, 2153 |
| BspACI | CCGC | 7 cut(s) | 60, 84, 101, 138, 967, 1283, 1592 |
| BspANI | GGCC | 6 cut(s) | 5, 59, 168, 503, 2095, 2114 |
| BspCNI | CTCAG | 1 cut(s) | 2182 |
| BspDI | ATCGAT | 1 cut(s) | 939 |
| BspFNI | CGCG | 4 cut(s) | 86, 632, 950, 1756 |
| BspLI | GGNNCC | 5 cut(s) | 914, 1255, 1342, 1414, 1821 |
| BspMAI | CTGCAG | 1 cut(s) | 877 |
| BspMI | ACCTGC | 1 cut(s) | 866 |
| BspOI | GCTAGC | 1 cut(s) | 80 |
| BspPI | GGATC | 1 cut(s) | 1594 |
| BspT107I | GGYRCC | 1 cut(s) | 1412 |
| BsrDI | GCAATG | 1 cut(s) | 71 |
| BsrI | ACTGG | 2 cut(s) | 647, 889 |
| BssECI | CCNNGG | 8 cut(s) | 169, 433, 922, 1257, 1546, 2115, 2153, 2181 |
| BssMI | GATC | 5 cut(s) | 214, 637, 1045, 1586, 1771 |
| BssNI | GRCGYC | 1 cut(s) | 1413 |
| BssSI | CACGAG | 1 cut(s) | 1012 |
| BssT1I | CCWWGG | 5 cut(s) | 169, 433, 1546, 2115, 2153 |
| Bst2BI | CACGAG | 1 cut(s) | 1012 |
| Bst2UI | CCWGG | 3 cut(s) | 120, 900, 1072 |
| Bst4CI | ACNGT | 4 cut(s) | 257, 305, 1214, 1354 |
| BstACI | GRCGYC | 1 cut(s) | 1413 |
| BstBAI | YACGTR | 1 cut(s) | 975 |
| BstC8I | GCNNGC | 3 cut(s) | 78, 896, 2294 |
| BstDEI | CTNAG | 1 cut(s) | 2190 |
| BstDSI | CCRYGG | 4 cut(s) | 1257, 1546, 2115, 2153 |
| BstEII | GGTNACC | 1 cut(s) | 2205 |
| BstF5I | GGATG | 5 cut(s) | 128, 478, 573, 1059, 2149 |
| BstFNI | CGCG | 4 cut(s) | 86, 632, 950, 1756 |
| BstH2I | RGCGCY | 1 cut(s) | 1416 |
| BstHHI | GCGC | 4 cut(s) | 88, 563, 950, 1415 |
| BstKTI | GATC | 5 cut(s) | 217, 640, 1048, 1589, 1774 |
| BstMAI | GTCTC | 4 cut(s) | 860, 1502, 1680, 2180 |
| BstMBI | GATC | 5 cut(s) | 214, 637, 1045, 1586, 1771 |
| BstMCI | CGRYCG | 2 cut(s) | 847, 967 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 558, 900, 969, 2057, 2155 |
| BstNI | CCWGG | 3 cut(s) | 120, 900, 1072 |
| BstNSI | RCATGY | 1 cut(s) | 2296 |
| BstPI | GGTNACC | 1 cut(s) | 2205 |
| BstSCI | CCNGG | 4 cut(s) | 118, 898, 1000, 1070 |
| BstSFI | CTRYAG | 1 cut(s) | 873 |
| BstSLI | GKGCMC | 1 cut(s) | 1642 |
| BstUI | CGCG | 4 cut(s) | 86, 632, 950, 1756 |
| BstV1I | GCAGC | 7 cut(s) | 21, 92, 375, 384, 675, 757, 956 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 1071, 2122 |
| Bsu15I | ATCGAT | 1 cut(s) | 939 |
| BsuRI | GGCC | 6 cut(s) | 5, 59, 168, 503, 2095, 2114 |
| BsuTUI | ATCGAT | 1 cut(s) | 939 |
| BtgI | CCRYGG | 4 cut(s) | 1257, 1546, 2115, 2153 |
| BtrI | CACGTC | 1 cut(s) | 1690 |
| BtsCI | GGATG | 5 cut(s) | 128, 478, 573, 1059, 2149 |
| BtsIMutI | CAGTG | 2 cut(s) | 640, 896 |
| BveI | ACCTGC | 1 cut(s) | 866 |
| Cac8I | GCNNGC | 3 cut(s) | 78, 896, 2294 |
| CaiI | CAGNNNCTG | 1 cut(s) | 895 |
| CfoI | GCGC | 4 cut(s) | 88, 563, 950, 1415 |
| Cfr13I | GGNCC | 1 cut(s) | 1819 |
| ClaI | ATCGAT | 1 cut(s) | 939 |
| CseI | GACGC | 2 cut(s) | 1943, 2012 |
| Csp6I | GTAC | 4 cut(s) | 258, 1198, 2194, 2271 |
| CviQI | GTAC | 4 cut(s) | 258, 1198, 2194, 2271 |
| DdeI | CTNAG | 1 cut(s) | 2190 |
| DinI | GGCGCC | 1 cut(s) | 1414 |
| DpnI | GATC | 5 cut(s) | 216, 639, 1047, 1588, 1773 |
| DpnII | GATC | 5 cut(s) | 214, 637, 1045, 1586, 1771 |
| EaeI | YGGCCR | 2 cut(s) | 57, 2112 |
| Ecl136II | GAGCTC | 1 cut(s) | 145 |
| Eco130I | CCWWGG | 5 cut(s) | 169, 433, 1546, 2115, 2153 |
| Eco147I | AGGCCT | 1 cut(s) | 2095 |
| Eco24I | GRGCYC | 1 cut(s) | 147 |
| Eco32I | GATATC | 1 cut(s) | 937 |
| Eco47I | GGWCC | 1 cut(s) | 1819 |
| Eco53kI | GAGCTC | 1 cut(s) | 145 |
| Eco57I | CTGAAG | 3 cut(s) | 1018, 1533, 1953 |
| Eco88I | CYCGRG | 3 cut(s) | 194, 383, 2180 |
| Eco91I | GGTNACC | 1 cut(s) | 2205 |
| EcoICRI | GAGCTC | 1 cut(s) | 145 |
| EcoO65I | GGTNACC | 1 cut(s) | 2205 |
| EcoRII | CCWGG | 3 cut(s) | 118, 898, 1070 |
| EcoRV | GATATC | 1 cut(s) | 937 |
| EcoT14I | CCWWGG | 5 cut(s) | 169, 433, 1546, 2115, 2153 |
| EcoT38I | GRGCYC | 1 cut(s) | 147 |
| EgeI | GGCGCC | 1 cut(s) | 1414 |
| EheI | GGCGCC | 1 cut(s) | 1414 |
| ErhI | CCWWGG | 5 cut(s) | 169, 433, 1546, 2115, 2153 |
| Esp3I | CGTCTC | 2 cut(s) | 860, 1680 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 1184, 1216, 1791, 1823 |
| FaqI | GGGAC | 2 cut(s) | 791, 1816 |
| FauNDI | CATATG | 1 cut(s) | 2317 |
| FbaI | TGATCA | 1 cut(s) | 637 |
| FblI | GTMKAC | 1 cut(s) | 1661 |
| FokI | GGATG | 5 cut(s) | 135, 485, 560, 1046, 2136 |
| FriOI | GRGCYC | 1 cut(s) | 147 |
| GlaI | GCGC | 4 cut(s) | 87, 562, 949, 1414 |
| GsaI | CCCAGC | 1 cut(s) | 2204 |
| HaeII | RGCGCY | 1 cut(s) | 1416 |
| HaeIII | GGCC | 6 cut(s) | 5, 59, 168, 503, 2095, 2114 |
| HapII | CCGG | 4 cut(s) | 489, 752, 847, 1002 |
| HgaI | GACGC | 2 cut(s) | 1943, 2012 |
| HhaI | GCGC | 4 cut(s) | 88, 563, 950, 1415 |
| Hin1I | GRCGYC | 1 cut(s) | 1413 |
| Hin6I | GCGC | 4 cut(s) | 86, 561, 948, 1413 |
| HinP1I | GCGC | 4 cut(s) | 86, 561, 948, 1413 |
| HincII | GTYRAC | 1 cut(s) | 301 |
| HindII | GTYRAC | 1 cut(s) | 301 |
| HindIII | AAGCTT | 3 cut(s) | 177, 1117, 2223 |
| HinfI | GANTC | 4 cut(s) | 22, 539, 1327, 1804 |
| HpaII | CCGG | 4 cut(s) | 489, 752, 847, 1002 |
| Hpy166II | GTNNAC | 7 cut(s) | 201, 301, 380, 584, 1357, 1662, 1867 |
| Hpy188I | TCNGA | 5 cut(s) | 514, 1037, 1627, 1741, 2221 |
| Hpy188III | TCNNGA | 4 cut(s) | 1138, 1507, 1685, 2000 |
| Hpy8I | GTNNAC | 7 cut(s) | 201, 301, 380, 584, 1357, 1662, 1867 |
| HpyAV | CCTTC | 9 cut(s) | 271, 405, 680, 902, 1167, 1291, 1331, 2106, 2252 |
| HpyCH4III | ACNGT | 4 cut(s) | 257, 305, 1214, 1354 |
| HpyCH4IV | ACGT | 4 cut(s) | 444, 974, 1689, 1863 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 558, 900, 969, 2057, 2155 |
| HpyF3I | CTNAG | 1 cut(s) | 2190 |
| HpySE526I | ACGT | 4 cut(s) | 444, 974, 1689, 1863 |
| Hsp92I | GRCGYC | 1 cut(s) | 1413 |
| HspAI | GCGC | 4 cut(s) | 86, 561, 948, 1413 |
| KasI | GGCGCC | 1 cut(s) | 1412 |
| Ksp22I | TGATCA | 1 cut(s) | 637 |
| Kzo9I | GATC | 5 cut(s) | 214, 637, 1045, 1586, 1771 |
| LmnI | GCTCC | 4 cut(s) | 849, 1253, 1340, 1748 |
| Lsp1109I | GCAGC | 7 cut(s) | 21, 92, 375, 384, 675, 757, 956 |
| LweI | GCATC | 8 cut(s) | 113, 555, 656, 1068, 1631, 1934, 2158, 2314 |
| MaeII | ACGT | 4 cut(s) | 444, 974, 1689, 1863 |
| MaeIII | GTNAC | 7 cut(s) | 28, 309, 440, 878, 1022, 1859, 2205 |
| MalI | GATC | 5 cut(s) | 216, 639, 1047, 1588, 1773 |
| MboI | GATC | 5 cut(s) | 214, 637, 1045, 1586, 1771 |
| MboII | GAAGA | 5 cut(s) | 695, 811, 1298, 1627, 1959 |
| MhlI | GDGCHC | 3 cut(s) | 147, 854, 1642 |
| MlsI | TGGCCA | 1 cut(s) | 2114 |
| MluNI | TGGCCA | 1 cut(s) | 2114 |
| Mly113I | GGCGCC | 1 cut(s) | 1413 |
| MlyI | GAGTC | 2 cut(s) | 548, 1798 |
| MmeI | TCCRAC | 1 cut(s) | 2103 |
| Mox20I | TGGCCA | 1 cut(s) | 2114 |
| MroXI | GAANNNNTTC | 2 cut(s) | 1199, 1830 |
| MscI | TGGCCA | 1 cut(s) | 2114 |
| MseI | TTAA | 8 cut(s) | 47, 618, 762, 1220, 1440, 1898, 2241, 2309 |
| MslI | CAYNNNNRTG | 2 cut(s) | 1320, 1476 |
| Msp20I | TGGCCA | 1 cut(s) | 2114 |
| MspA1I | CMGCKG | 2 cut(s) | 969, 1283 |
| MspI | CCGG | 4 cut(s) | 489, 752, 847, 1002 |
| MspR9I | CCNGG | 4 cut(s) | 120, 900, 1002, 1072 |
| Mva1269I | GAATGC | 1 cut(s) | 2136 |
| MvaI | CCWGG | 3 cut(s) | 120, 900, 1072 |
| MvnI | CGCG | 4 cut(s) | 86, 632, 950, 1756 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 558, 900, 969, 2057, 2155 |
| NarI | GGCGCC | 1 cut(s) | 1413 |
| NciI | CCSGG | 1 cut(s) | 1002 |
| NcoI | CCATGG | 3 cut(s) | 1546, 2115, 2153 |
| NdeI | CATATG | 1 cut(s) | 2317 |
| NdeII | GATC | 5 cut(s) | 214, 637, 1045, 1586, 1771 |
| NheI | GCTAGC | 1 cut(s) | 76 |
| NlaIV | GGNNCC | 5 cut(s) | 914, 1255, 1342, 1414, 1821 |
| NmeAIII | GCCGAG | 1 cut(s) | 956 |
| NmuCI | GTSAC | 2 cut(s) | 1859, 2205 |
| NspI | RCATGY | 1 cut(s) | 2296 |
| PaeI | GCATGC | 1 cut(s) | 2296 |
| PaeR7I | CTCGAG | 1 cut(s) | 383 |
| PceI | AGGCCT | 1 cut(s) | 2095 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 675 |
| PctI | GAATGC | 1 cut(s) | 2136 |
| PdmI | GAANNNNTTC | 2 cut(s) | 1199, 1830 |
| PfeI | GAWTC | 2 cut(s) | 22, 1327 |
| PflFI | GACNNNGTC | 1 cut(s) | 538 |
| PfoI | TCCNGGA | 1 cut(s) | 1000 |
| PleI | GAGTC | 2 cut(s) | 547, 1798 |
| PluTI | GGCGCC | 1 cut(s) | 1416 |
| PpsI | GAGTC | 2 cut(s) | 547, 1798 |
| Ppu21I | YACGTR | 1 cut(s) | 975 |
| Psp124BI | GAGCTC | 1 cut(s) | 147 |
| Psp1406I | AACGTT | 1 cut(s) | 444 |
| Psp6I | CCWGG | 3 cut(s) | 118, 898, 1070 |
| PspEI | GGTNACC | 1 cut(s) | 2205 |
| PspFI | CCCAGC | 1 cut(s) | 2200 |
| PspGI | CCWGG | 3 cut(s) | 118, 898, 1070 |
| PspN4I | GGNNCC | 5 cut(s) | 914, 1255, 1342, 1414, 1821 |
| PspPI | GGNCC | 1 cut(s) | 1819 |
| PspXI | VCTCGAGB | 1 cut(s) | 383 |
| PstI | CTGCAG | 1 cut(s) | 877 |
| PstNI | CAGNNNCTG | 1 cut(s) | 895 |
| PsyI | GACNNNGTC | 1 cut(s) | 538 |
| RsaI | GTAC | 4 cut(s) | 259, 1199, 2195, 2272 |
| RsaNI | GTAC | 4 cut(s) | 258, 1198, 2194, 2271 |
| RseI | CAYNNNNRTG | 2 cut(s) | 1320, 1476 |
| SacI | GAGCTC | 1 cut(s) | 147 |
| SaqAI | TTAA | 8 cut(s) | 47, 618, 762, 1220, 1440, 1898, 2241, 2309 |
| Sau3AI | GATC | 5 cut(s) | 214, 637, 1045, 1586, 1771 |
| Sau96I | GGNCC | 1 cut(s) | 1819 |
| ScaI | AGTACT | 2 cut(s) | 1199, 2195 |
| SchI | GAGTC | 2 cut(s) | 548, 1798 |
| ScrFI | CCNGG | 4 cut(s) | 120, 900, 1002, 1072 |
| SduI | GDGCHC | 3 cut(s) | 147, 854, 1642 |
| SfaNI | GCATC | 8 cut(s) | 113, 555, 656, 1068, 1631, 1934, 2158, 2314 |
| SfcI | CTRYAG | 1 cut(s) | 873 |
| SfoI | GGCGCC | 1 cut(s) | 1414 |
| Sfr274I | CTCGAG | 1 cut(s) | 383 |
| SinI | GGWCC | 1 cut(s) | 1819 |
| SlaI | CTCGAG | 1 cut(s) | 383 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 1320, 1476 |
| SmlI | CTYRAG | 3 cut(s) | 108, 383, 1292 |
| SmoI | CTYRAG | 3 cut(s) | 108, 383, 1292 |
| SpeI | ACTAGT | 2 cut(s) | 1525, 1792 |
| SphI | GCATGC | 1 cut(s) | 2296 |
| SseBI | AGGCCT | 1 cut(s) | 2095 |
| SsiI | CCGC | 7 cut(s) | 60, 84, 101, 138, 967, 1283, 1592 |
| SspDI | GGCGCC | 1 cut(s) | 1412 |
| SstI | GAGCTC | 1 cut(s) | 147 |
| StuI | AGGCCT | 1 cut(s) | 2095 |
| StyD4I | CCNGG | 4 cut(s) | 118, 898, 1000, 1070 |
| StyI | CCWWGG | 5 cut(s) | 169, 433, 1546, 2115, 2153 |
| TaaI | ACNGT | 4 cut(s) | 257, 305, 1214, 1354 |
| TaiI | ACGT | 4 cut(s) | 447, 977, 1692, 1866 |
| TaqI | TCGA | 5 cut(s) | 384, 843, 939, 1506, 1684 |
| TaqII | GACCGA | 1 cut(s) | 551 |
| TatI | WGTACW | 2 cut(s) | 1197, 2193 |
| TauI | GCSGC | 3 cut(s) | 62, 86, 103 |
| TfiI | GAWTC | 2 cut(s) | 22, 1327 |
| Tru1I | TTAA | 8 cut(s) | 47, 618, 762, 1220, 1440, 1898, 2241, 2309 |
| Tru9I | TTAA | 8 cut(s) | 47, 618, 762, 1220, 1440, 1898, 2241, 2309 |
| TscAI | CASTG | 2 cut(s) | 647, 896 |
| TseFI | GTSAC | 2 cut(s) | 1859, 2205 |
| TseI | GCWGC | 7 cut(s) | 34, 80, 372, 388, 663, 745, 969 |
| Tsp45I | GTSAC | 2 cut(s) | 1859, 2205 |
| TspDTI | ATGAA | 8 cut(s) | 588, 664, 1178, 1587, 1750, 1823, 2117, 2271 |
| TspRI | CASTG | 2 cut(s) | 647, 896 |
| Tth111I | GACNNNGTC | 1 cut(s) | 538 |
| VpaK11BI | GGWCC | 1 cut(s) | 1819 |
| XapI | RAATTY | 4 cut(s) | 991, 1995, 2170, 2298 |
| XceI | RCATGY | 1 cut(s) | 2296 |
| XhoI | CTCGAG | 1 cut(s) | 383 |
| XmaJI | CCTAGG | 1 cut(s) | 169 |
| XmiI | GTMKAC | 1 cut(s) | 1661 |
| XmnI | GAANNNNTTC | 2 cut(s) | 1199, 1830 |
| ZrmI | AGTACT | 2 cut(s) | 1199, 2195 |