Prupe.4G091900_v2.0.a1

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0000066
Physical Location & Seq
Reverse (-)
4586620 .. 4589077
2458 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G091900.1

Sequence Viewer

Length: 2265 bp
ATGGCCTTACATGGGATTATTCTGCAACTCTTCTTAGTGGGATTGGTTGTATTAGCAGCTGCAGAAGCTCAAGCCTTGCCTGGCTGCCCAAACCAATGTGGAAATTTGTCAATTCCATTTCCATTTGGCATAGCTAAAGGCTGTTACCTCAGAGATGAATTTTTCATTGATTGTAATGAAACCAACCAAACCCCAACACCATATTTGAATGGAACTGGGATCCCAATTTCCAACTTATCCCTTAATGGTGAGTTACAAATAATGCAATTTGTAGCCAGGGATTGCTATGACCAAGATGGTTCTTTAGACACAAAACTCAGCAACACCCCAAGGCTCAAGCTTTTCCCTCCTTACACAATCTCCGGCACCAAAAACAAGTTCATTGCCGTTGGTTGTGACACTTACGCAATCTTCGAAGGTGGTCGGGGCAAAGAAAAGTACATAACTGGGTGCATGACCTTCTGTGAGAGCCTTGGAAGCATTAGTGAGTCTTGCTCTGGCATTGGGTGCTGCCAAACTTCAATCCCAAGTGGACTTCAAGTGCGTACCGTGACAATGAGTAGTTACTACAATCATACATTTATATGGGACTTCAACCCCTGCAGCTACTCCTTCATTGTGGAAGAAGGCCAGTTCACATTCTCTAGTAAAAGTTTCCAAGAACTGAAATCTATAAGCCGGCTTCCGATGGTTCTCAACTGGGCAATTGGGGATGAACCATGTGATGCAGCTCAACATAGGCAGGATTATGCATGCAAGGGAAATAGCACATGTGTCAACCCCCTCAATCTGTCTGGTTATTTTTGTGAATGTTTGCCAGGTTATGAAGGAAATCCATACCTCCCAGACGGTTGTCAAGATACCGACGAGTGCCAGATTTCAAACCCCTGCAGTGCAGGAGCGTGTGTAAACGTACTTGGAAATTATTCTTGTGTATGTCCAAAAGGTTTCAAAGGCGACGGAATGAAAGCTGGAACAGGTTGCAGCAAAGACAACACCAGCAATCTTTTTAAGGGCATTCACTTGCTTACTATTTCATTGGCTATGACAGTAGCCTTGTTGGTTTTGCTGGTTGGAAGTTCATGGACTTATTGGGGAACGAAGAAAAGGAGGTTCATCAAACTCAAAGAAAAATACTTTCAAGAAAATGGTGGCTTCTTGTTGCAGCAACAACTCGCTAGTCGCAGAGGACCTGTGCAGACAACGAAAATTTTTACTGCAGAAGAACTCGAGAAGGCTACAAACAATTACCATGAAAGCAGAGTCCTTGGTGAAGGAGGATATGGAACAGTGTACAAAGGAATACTAGAAGATGACAAAGTGGTTGCCATAAAAAAGTCCAAGATTTGTGCCCCCGCTCAGAATGAGCAATTTGTTAACGAGGTGATTGTTCTTTCTCAAATCAACCATAGGAATGTGGTGAGGCTGTTAGGTTGTTGTTTAGAGACGCCGATGCCATTGTTGGTTTACGAGTTCATCATCAACGGGACTTTGTCGGAGCATATTCACAACAAATGCAGAGAATCTTTACTTTCATGGGAGCTGCGGTTGAAGATAGCCGCAGAGACTGCAGGAGCACTGGCATACTTACACTCCTCCATTTCCATCCCGATCATACACCGAGATGTGAAGACGACGAATGTACTCTTAGATGAAAATTACATAGCAAAAGTGTCTGACTTCGGAGCTTCACGGTTGATTCCTCTAGATCAAGCTCAAATAACTACATTAGTGCAAGGAACACTCGGGTACTTGGACCCTGAATACTTCCATTCAAACCAACTAACGGAAAAGAGTGACGTGTATAGCTTTGGAGTTGTCCTTGCAGAGCTACTAACAAGCAAAGTGGCACTTTCTTTTGCCAGGCCTGAGGCAGAGAGAAATCTAGCGAGCTTCTTTGTTTGTTCAGTGGAACAGGGTCGCTTGAATCAAATTCTTGATGAAGACATAGTCAATGAGGGAAATATTGAGACACTTAAAAAAGTGGCTGATCTTGCAAATAGATGTTTAAGGGTAAAAAGGGAGGAAAGGCCTACCATGAAACACATAGCCATGGAGCTGGAGGGGATGATGATTATGGCAAAGCATCCATGGGGAAAGGCCAATTTCGGCCCAGAGGACACTGAATACTTACTTGGGTCAGCTTCAATGGCTTCTGTTAGAGGTGACAATTGTTCTAGTACTGGTACAACTACTGATGCAACTACTGTGTACGACAGCATGCGAATCGAAATCAAAATGTTAATGCGACACAACGGGCGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK

Protein Analysis

755

Amino Acids

83.19

Weight (kDa)

5.87

Isoelectric Point (pI)

28.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21210 AT1G21230 AT1G21240 AT1G21240 AT1G21245 AT1G21250 AT1G21270 AT1G22720 AT3G25490
fragaria_vesca FvH4_2g35051 FvH4_2g35051 FvH4_2g35060 FvH4_3g10281 FvH4_3g10285 FvH4_3g10286 FvH4_3g10311 FvH4_3g10311 FvH4_3g10320 FvH4_3g10332 FvH4_3g10340 FvH4_3g10350 FvH4_3g10360 FvH4_3g10390 FvH4_3g10412 FvH4_3g10413 FvH4_3g10461 FvH4_3g10462 FvH4_3g10470 FvH4_3g10510 FvH4_3g10520 FvH4_3g10530 FvH4_3g10550 FvH4_3g10560 FvH4_3g10611 FvH4_3g11730 FvH4_6g53140 FvH4_6g53142 FvH4_7g31880 FvH4_7g31910 FvH4_7g31920
malus_domestica MD05G1269900.v1.1 MD05G1270000.v1.1 MD05G1270200.v1.1 MD05G1270400.v1.1 MD05G1270500.v1.1 MD05G1270600.v1.1 MD05G1270800.v1.1 MD05G1270900.v1.1 MD07G1183800.v1.1 MD08G1107600.v1.1 MD10G1248500.v1.1 MD10G1249200.v1.1 MD10G1249500.v1.1 MD10G1250000.v1.1 MD10G1250500.v1.1 MD10G1250900.v1.1 MD10G1251200.v1.1 MD10G1251400.v1.1 MD13G1004600.v1.1 MD15G1088400.v1.1
prunus_persica Prupe.1G442400_v2.0.a1 Prupe.1G442500_v2.0.a1 Prupe.2G223400_v2.0.a1 Prupe.4G091900_v2.0.a1 Prupe.4G093200_v2.0.a1 Prupe.4G093300_v2.0.a1 Prupe.4G093400_v2.0.a1 Prupe.4G093500_v2.0.a1 Prupe.4G094100_v2.0.a1 Prupe.4G094200_v2.0.a1 Prupe.4G094300_v2.0.a1 Prupe.6G358300_v2.0.a1
pyrus_communis pycom08g08990 pycom10g20660 pycom10g20670 pycom10g20680 pycom10g20710 pycom10g20740 pycom13g00400 pycom15g08290
rosa_chinensis RchiOBHm_Chr1g0326861 RchiOBHm_Chr1g0326871 RchiOBHm_Chr1g0331391 RchiOBHm_Chr1g0336911 RchiOBHm_Chr1g0364001 RchiOBHm_Chr2g0174541 RchiOBHm_Chr2g0175041 RchiOBHm_Chr2g0175101 RchiOBHm_Chr2g0175121 RchiOBHm_Chr3g0486331 RchiOBHm_Chr5g0015581 RchiOBHm_Chr5g0016551 RchiOBHm_Chr5g0016561 RchiOBHm_Chr5g0016581 RchiOBHm_Chr5g0016601 RchiOBHm_Chr5g0016611 RchiOBHm_Chr5g0016641 RchiOBHm_Chr5g0016661 RchiOBHm_Chr5g0016691 RchiOBHm_Chr5g0016701 RchiOBHm_Chr5g0016731 RchiOBHm_Chr5g0016741 RchiOBHm_Chr5g0016811 RchiOBHm_Chr5g0016841 RchiOBHm_Chr5g0016871 RchiOBHm_Chr5g0016881 RchiOBHm_Chr5g0016931 RchiOBHm_Chr5g0017021 RchiOBHm_Chr5g0017041 RchiOBHm_Chr5g0017071 RchiOBHm_Chr5g0017091 RchiOBHm_Chr5g0017121 RchiOBHm_Chr5g0017141 RchiOBHm_Chr5g0017291 RchiOBHm_Chr5g0017321 RchiOBHm_Chr5g0017401 RchiOBHm_Chr5g0019001 RchiOBHm_Chr5g0019071 RchiOBHm_Chr5g0028941 RchiOBHm_Chr5g0052141 RchiOBHm_Chr5g0054721 RchiOBHm_Chr5g0054801 RchiOBHm_Chr5g0054931 RchiOBHm_Chr5g0054941 RchiOBHm_Chr6g0259291 RchiOBHm_Chr6g0292991 RchiOBHm_Chr6g0306911
rosa_laevigata RLG00000009465 RLG00000009468 RLG00000010755 RLG00000011976 RLG00000014541 RLG00000014542 RLG00000015169 RLG00000022308 RLG00000027529 RLG00000029324 RLG00000030244 RLG00000030245 RLG00000032210 RLG00000032211 RLG00000032257 RLG00000032259 RLG00000032260 RLG00000032261 RLG00000032262 RLG00000032268 RLG00000032269 RLG00000032272 RLG00000032282 RLG00000032283 RLG00000032308 RLG00000032413 RLG00000033129 RLG00000034945
rosa_multiflora Rmu_co8241929.1_g000001 Rmu_co8269227.1_g000001 Rmu_co8461259.1_g000001 Rmu_co8495385.1_g000001 Rmu_sc0000028.1_g000003 Rmu_sc0000083.1_g000009 Rmu_sc0000083.1_g000010 Rmu_sc0000083.1_g000031 Rmu_sc0000083.1_g000043 Rmu_sc0000083.1_g000046 Rmu_sc0000083.1_g000047 Rmu_sc0000563.1_g000024 Rmu_sc0000842.1_g000012 Rmu_sc0001235.1_g000001 Rmu_sc0001235.1_g000005 Rmu_sc0001235.1_g000009 Rmu_sc0001235.1_g000015 Rmu_sc0001235.1_g000016 Rmu_sc0001235.1_g000017 Rmu_sc0001473.1_g000036 Rmu_sc0001607.1_g000016 Rmu_sc0001788.1_g000006 Rmu_sc0002018.1_g000008 Rmu_sc0002028.1_g000001 Rmu_sc0002680.1_g000011 Rmu_sc0002798.1_g000023 Rmu_sc0002886.1_g000023 Rmu_sc0002989.1_g000012 Rmu_sc0003064.1_g000009 Rmu_sc0003382.1_g000006 Rmu_sc0003630.1_g000002 Rmu_sc0003630.1_g000047 Rmu_sc0003630.1_g000066 Rmu_sc0003630.1_g000067 Rmu_sc0003649.1_g000010 Rmu_sc0003688.1_g000005 Rmu_sc0003920.1_g000007 Rmu_sc0004765.1_g000007 Rmu_sc0004765.1_g000008 Rmu_sc0004920.1_g000011 Rmu_sc0005223.1_g000008 Rmu_sc0005223.1_g000010 Rmu_sc0005223.1_g000015 Rmu_sc0005223.1_g000016 Rmu_sc0005319.1_g000014 Rmu_sc0005319.1_g000015 Rmu_sc0005319.1_g000018 Rmu_sc0005319.1_g000019 Rmu_sc0005319.1_g000025 Rmu_sc0005418.1_g000007 Rmu_sc0005418.1_g000012 Rmu_sc0006106.1_g000011 Rmu_sc0006914.1_g000012 Rmu_sc0007023.1_g000001 Rmu_sc0007662.1_g000001 Rmu_sc0007920.1_g000002 Rmu_sc0007920.1_g000013 Rmu_sc0008053.1_g000016 Rmu_sc0008053.1_g000017 Rmu_sc0008053.1_g000018 Rmu_sc0008053.1_g000023 Rmu_sc0008053.1_g000050 Rmu_sc0009042.1_g000007 Rmu_sc0009042.1_g000008 Rmu_sc0010642.1_g000008 Rmu_sc0011109.1_g000002 Rmu_sc0011314.1_g000006 Rmu_sc0011974.1_g000006 Rmu_sc0012179.1_g000009 Rmu_sc0015169.1_g000001 Rmu_sc0016368.1_g000002 Rmu_sc0022659.1_g000001 Rmu_sc0027371.1_g000001 Rmu_sc0028213.1_g000001 Rmu_sc0034526.1_g000001 Rmu_sc0039366.1_g000002 Rmu_ssc0000050.1_g000084 Rmu_ssc0000050.1_g000088 Rmu_ssc0000167.1_g000017
rosa_roxburghii Rroxscaffold_1G00011340 Rroxscaffold_1G00025710 Rroxscaffold_1G00025870 Rroxscaffold_1G00026070 Rroxscaffold_1G00026170 Rroxscaffold_1G00026200 Rroxscaffold_1G00026270 Rroxscaffold_1G00050950 Rroxscaffold_1G00058900 Rroxscaffold_1G00060040 Rroxscaffold_1G00060060 Rroxscaffold_1G00060070 Rroxscaffold_1G00060080 Rroxscaffold_1G00060110 Rroxscaffold_1G00060120 Rroxscaffold_1G00060140 Rroxscaffold_1G00060170 Rroxscaffold_1G00060210 Rroxscaffold_1G00060220 Rroxscaffold_1G00060240 Rroxscaffold_1G00060250 Rroxscaffold_1G00060260 Rroxscaffold_1G00060300 Rroxscaffold_1G00060320 Rroxscaffold_1G00060330 Rroxscaffold_1G00060900 Rroxscaffold_1G00060920 Rroxscaffold_1G00073980 Rroxscaffold_2G00077560 Rroxscaffold_2G00077580 Rroxscaffold_2G00146990 Rroxscaffold_3G00236200 Rroxscaffold_3G00236210 Rroxscaffold_3G00236220 Rroxscaffold_4G00292760 Rroxscaffold_4G00315200 Rroxscaffold_4G00315240 Rroxscaffold_4G00326240 Rroxscaffold_4G00328980 Rroxscaffold_5G00341890 Rroxscaffold_7G00161350 Rroxscaffold_7G00174490 Rroxscaffold_7G00206550
rosa_rugosa Rorug01G0093000 Rorug01G0093100 Rorug01G0128800 Rorug01G0312000 Rorug01G0312000 Rorug02G0581100 Rorug03G0224600 Rorug05G0028500 Rorug05G0032700 Rorug05G0032800 Rorug05G0032900 Rorug05G0032900 Rorug05G0033000 Rorug05G0033100 Rorug05G0033300 Rorug05G0033400 Rorug05G0033900 Rorug05G0034900 Rorug05G0035000 Rorug05G0046900 Rorug05G0047500 Rorug05G0047500 Rorug05G0111600 Rorug05G0287900 Rorug05G0288700 Rorug05G0586900 Rorug06G0231700 Rorug06G0354300 Rorug06G0354400
rosa_samantha Rh1DG122200 Rh2DG686800 Rh5BG136600 Rh6AG344500 Rh6BG351900 Rh6CG359000 Rh6CG359100 Rh6CG387600 Rh6CG480500 Rh6DG344600 Rh6DG467400
rosa_wichuraiana Rw0G010250 Rw0G014820 Rw0G021400 Rw0G023100 Rw1G009450 Rw1G012100 Rw1G012180 Rw1G028360 Rw2G054260 Rw3G024370 Rw3G024420 Rw3G024470 Rw5G010850 Rw5G011010 Rw5G011020 Rw5G011050 Rw5G011080 Rw5G011120 Rw5G012300 Rw5G018470 Rw5G033830 Rw6G008930 Rw6G030070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 365
AccBSI CCGCTC 1 cut(s) 1358
AciI CCGC 3 cut(s) 1356, 1546, 1560
AclWI GGATC 2 cut(s) 214, 227
AcsI RAATTY 4 cut(s) 103, 158, 1209, 1932
AcyI GRCGYC 1 cut(s) 1448
AfaI GTAC 9 cut(s) 440, 547, 915, 1295, 1644, 1751, 2182, 2188, 2213
AfiI CCNNNNNNNGG 2 cut(s) 12, 1786
AflIII ACRYGT 2 cut(s) 770, 1800
AjiI CACGTC 1 cut(s) 1801
AjnI CCWGG 4 cut(s) 79, 275, 817, 1862
AjuI GAANNNNNNNTTGG 4 cut(s) 362, 394, 2118, 2150
Alw21I GWGCWC 1 cut(s) 1579
Alw26I GTCTC 3 cut(s) 1439, 1559, 1964
AlwI GGATC 2 cut(s) 214, 227
AlwNI CAGNNNCTG 1 cut(s) 1568
Ama87I CYCGRG 2 cut(s) 1229, 1745
AoxI GGCC 6 cut(s) 3, 628, 1865, 2030, 2100, 2110
ApeKI GCWGC 9 cut(s) 56, 59, 84, 510, 603, 728, 984, 1165, 1543
ApoI RAATTY 4 cut(s) 103, 158, 1209, 1932
Asp700I GAANNNNTTC 1 cut(s) 925
AspS9I GGNCC 3 cut(s) 1190, 1756, 2111
AsuHPI GGTGA 5 cut(s) 260, 1283, 1396, 1432, 2177
AsuII TTCGAA 1 cut(s) 414
AvaI CYCGRG 2 cut(s) 1229, 1745
AvaII GGWCC 2 cut(s) 1190, 1756
AxyI CCTNAGG 1 cut(s) 1869
BaeGI GKGCMC 1 cut(s) 1354
BamHI GGATCC 1 cut(s) 219
BanI GGYRCC 1 cut(s) 365
BbsI GAAGAC 2 cut(s) 1637, 1950
Bbv12I GWGCWC 1 cut(s) 1579
BbvI GCAGC 9 cut(s) 46, 68, 71, 497, 615, 740, 996, 1177, 1530
BccI CCATC 3 cut(s) 290, 682, 1613
BceAI ACGGC 1 cut(s) 371
BcgI CGANNNNNNTGC 2 cut(s) 2209, 2243
BciT130I CCWGG 4 cut(s) 81, 277, 819, 1864
BcoDI GTCTC 3 cut(s) 1439, 1559, 1964
BfaI CTAG 6 cut(s) 645, 1179, 1307, 1706, 1886, 2178
BfmI CTRYAG 5 cut(s) 60, 601, 889, 1218, 1569
BmcAI AGTACT 1 cut(s) 2182
Bme1390I CCNGG 4 cut(s) 81, 277, 819, 1864
Bme18I GGWCC 2 cut(s) 1190, 1756
BmeT110I CYCGRG 2 cut(s) 1229, 1745
BmgBI CACGTC 1 cut(s) 1801
BmgT120I GGNCC 3 cut(s) 1190, 1756, 2111
BmiI GGNNCC 3 cut(s) 221, 367, 1758
BmrFI CCNGG 4 cut(s) 81, 277, 819, 1864
BmrI ACTGGG 3 cut(s) 225, 456, 709
BmsI GCATC 4 cut(s) 715, 1443, 2095, 2188
BmuI ACTGGG 3 cut(s) 225, 456, 709
BoxI GACNNNNGTC 1 cut(s) 852
BpiI GAAGAC 2 cut(s) 1637, 1950
BplI GAGNNNNNCTC 2 cut(s) 479, 511
BpmI CTGGAG 1 cut(s) 2081
Bpu14I TTCGAA 1 cut(s) 414
BpuEI CTTGAG 2 cut(s) 54, 320
BsaHI GRCGYC 1 cut(s) 1448
BsaJI CCNNGG 6 cut(s) 276, 329, 472, 1267, 2052, 2090
BsaXI ACNNNNNCTCC 8 cut(s) 344, 374, 891, 921, 1532, 1562, 1577, 1607
Bsc4I CCNNNNNNNGG 2 cut(s) 12, 1786
Bse118I RCCGGY 1 cut(s) 678
Bse1I ACTGG 6 cut(s) 220, 451, 631, 704, 1584, 2188
Bse21I CCTNAGG 1 cut(s) 1869
Bse3DI GCAATG 1 cut(s) 381
BseBI CCWGG 4 cut(s) 81, 277, 819, 1864
BseDI CCNNGG 6 cut(s) 276, 329, 472, 1267, 2052, 2090
BseGI GGATG 4 cut(s) 718, 1605, 2073, 2086
BseLI CCNNNNNNNGG 2 cut(s) 12, 1786
BseMI GCAATG 1 cut(s) 381
BseMII CTCAG 4 cut(s) 163, 331, 1373, 1860
BseNI ACTGG 6 cut(s) 220, 451, 631, 704, 1584, 2188
BseRI GAGGAG 1 cut(s) 1585
BseSI GKGCMC 1 cut(s) 1354
BseXI GCAGC 9 cut(s) 46, 68, 71, 497, 615, 740, 996, 1177, 1530
BsgI GTGCAG 2 cut(s) 915, 1217
BshFI GGCC 6 cut(s) 5, 630, 1867, 2032, 2102, 2112
BshNI GGYRCC 1 cut(s) 365
BsiHKAI GWGCWC 1 cut(s) 1579
BsiHKCI CYCGRG 2 cut(s) 1229, 1745
BsiSI CCGG 2 cut(s) 363, 679
BslFI GGGAC 2 cut(s) 602, 1501
BslI CCNNNNNNNGG 2 cut(s) 12, 1786
BsmAI GTCTC 3 cut(s) 1439, 1559, 1964
BsmBI CGTCTC 1 cut(s) 1439
BsmFI GGGAC 2 cut(s) 602, 1501
BsmI GAATGC 1 cut(s) 1017
BsnI GGCC 6 cut(s) 5, 630, 1867, 2032, 2102, 2112
BsoBI CYCGRG 2 cut(s) 1229, 1745
Bsp119I TTCGAA 1 cut(s) 414
Bsp1286I GDGCHC 2 cut(s) 1354, 1579
Bsp1407I TGTACA 1 cut(s) 1293
Bsp143I GATC 4 cut(s) 219, 1611, 1708, 1990
Bsp19I CCATGG 2 cut(s) 2052, 2090
BspACI CCGC 3 cut(s) 1356, 1546, 1560
BspANI GGCC 6 cut(s) 5, 630, 1867, 2032, 2102, 2112
BspCNI CTCAG 4 cut(s) 162, 330, 1372, 1861
BspLI GGNNCC 3 cut(s) 221, 367, 1758
BspMAI CTGCAG 5 cut(s) 64, 605, 893, 1222, 1573
BspPI GGATC 2 cut(s) 214, 227
BspT104I TTCGAA 1 cut(s) 414
BspT107I GGYRCC 1 cut(s) 365
BsrBI CCGCTC 1 cut(s) 1358
BsrDI GCAATG 1 cut(s) 381
BsrFI RCCGGY 1 cut(s) 678
BsrGI TGTACA 1 cut(s) 1293
BsrI ACTGG 6 cut(s) 220, 451, 631, 704, 1584, 2188
BssAI RCCGGY 1 cut(s) 678
BssECI CCNNGG 6 cut(s) 276, 329, 472, 1267, 2052, 2090
BssMI GATC 4 cut(s) 219, 1611, 1708, 1990
BssNI GRCGYC 1 cut(s) 1448
BssT1I CCWWGG 5 cut(s) 329, 472, 1267, 2052, 2090
Bst2UI CCWGG 4 cut(s) 81, 277, 819, 1864
Bst4CI ACNGT 6 cut(s) 550, 851, 1051, 1291, 1695, 2209
Bst6I CTCTTC 1 cut(s) 35
BstACI GRCGYC 1 cut(s) 1448
BstAPI GCANNNNNTGC 2 cut(s) 507, 1568
BstAUI TGTACA 1 cut(s) 1293
BstBI TTCGAA 1 cut(s) 414
BstC8I GCNNGC 4 cut(s) 680, 754, 1891, 2222
BstDEI CTNAG 6 cut(s) 34, 149, 317, 1359, 1648, 1869
BstDSI CCRYGG 2 cut(s) 2052, 2090
BstF5I GGATG 4 cut(s) 718, 1605, 2073, 2086
BstKTI GATC 4 cut(s) 222, 1614, 1711, 1993
BstMAI GTCTC 3 cut(s) 1439, 1559, 1964
BstMBI GATC 4 cut(s) 219, 1611, 1708, 1990
BstMWI GCNNNNNNNGC 6 cut(s) 65, 477, 507, 1568, 1994, 2150
BstNI CCWGG 4 cut(s) 81, 277, 819, 1864
BstNSI RCATGY 3 cut(s) 756, 774, 2224
BstPAI GACNNNNGTC 1 cut(s) 852
BstSCI CCNGG 4 cut(s) 79, 275, 817, 1862
BstSFI CTRYAG 5 cut(s) 60, 601, 889, 1218, 1569
BstSLI GKGCMC 1 cut(s) 1354
BstV1I GCAGC 9 cut(s) 46, 68, 71, 497, 615, 740, 996, 1177, 1530
BstV2I GAAGAC 2 cut(s) 1637, 1950
BstX2I RGATCY 1 cut(s) 219
BstXI CCANNNNNNTGG 1 cut(s) 2059
BstYI RGATCY 1 cut(s) 219
Bsu36I CCTNAGG 1 cut(s) 1869
BsuRI GGCC 6 cut(s) 5, 630, 1867, 2032, 2102, 2112
BtgI CCRYGG 2 cut(s) 2052, 2090
BtrI CACGTC 1 cut(s) 1801
BtsCI GGATG 4 cut(s) 718, 1605, 2073, 2086
BtsI GCAGTG 1 cut(s) 898
BtsIMutI CAGTG 5 cut(s) 898, 1296, 1577, 1914, 2121
Cac8I GCNNGC 4 cut(s) 680, 754, 1891, 2222
CaiI CAGNNNCTG 1 cut(s) 1568
Cfr10I RCCGGY 1 cut(s) 678
Cfr13I GGNCC 3 cut(s) 1190, 1756, 2111
CseI GACGC 1 cut(s) 1456
Csp6I GTAC 9 cut(s) 439, 546, 914, 1294, 1643, 1750, 2181, 2187, 2212
CspCI CAANNNNNGTGG 2 cut(s) 1827, 1862
CviQI GTAC 9 cut(s) 439, 546, 914, 1294, 1643, 1750, 2181, 2187, 2212
DdeI CTNAG 6 cut(s) 34, 149, 317, 1359, 1648, 1869
DpnI GATC 4 cut(s) 221, 1613, 1710, 1992
DpnII GATC 4 cut(s) 219, 1611, 1708, 1990
Eam1104I CTCTTC 1 cut(s) 35
EarI CTCTTC 1 cut(s) 35
Eco130I CCWWGG 5 cut(s) 329, 472, 1267, 2052, 2090
Eco147I AGGCCT 2 cut(s) 1867, 2032
Eco47I GGWCC 2 cut(s) 1190, 1756
Eco81I CCTNAGG 1 cut(s) 1869
Eco88I CYCGRG 2 cut(s) 1229, 1745
EcoO109I RGGNCCY 1 cut(s) 1190
EcoRII CCWGG 4 cut(s) 79, 275, 817, 1862
EcoT14I CCWWGG 5 cut(s) 329, 472, 1267, 2052, 2090
EcoT22I ATGCAT 1 cut(s) 754
ErhI CCWWGG 5 cut(s) 329, 472, 1267, 2052, 2090
Esp3I CGTCTC 1 cut(s) 1439
FalI AAGNNNNNCTT 2 cut(s) 1836, 1868
FaqI GGGAC 2 cut(s) 602, 1501
FauI CCCGC 1 cut(s) 1363
FokI GGATG 4 cut(s) 725, 1592, 2073, 2080
FspBI CTAG 6 cut(s) 645, 1179, 1307, 1706, 1886, 2178
GsuI CTGGAG 1 cut(s) 2081
HaeIII GGCC 6 cut(s) 5, 630, 1867, 2032, 2102, 2112
HapII CCGG 2 cut(s) 363, 679
HgaI GACGC 1 cut(s) 1456
Hin1I GRCGYC 1 cut(s) 1448
HincII GTYRAC 2 cut(s) 778, 1378
HindII GTYRAC 2 cut(s) 778, 1378
HindIII AAGCTT 1 cut(s) 338
HinfI GANTC 6 cut(s) 488, 1263, 1523, 1699, 1927, 2226
HpaI GTTAAC 1 cut(s) 1378
HpaII CCGG 2 cut(s) 363, 679
HphI GGTGA 5 cut(s) 260, 1283, 1396, 1432, 2177
Hpy166II GTNNAC 8 cut(s) 533, 636, 778, 910, 1294, 1378, 1468, 2212
Hpy188I TCNGA 6 cut(s) 152, 687, 1362, 1498, 1678, 1685
Hpy188III TCNNGA 6 cut(s) 857, 1142, 1231, 1609, 1706, 1937
Hpy8I GTNNAC 8 cut(s) 533, 636, 778, 910, 1294, 1378, 1468, 2212
Hpy99I CGWCG 3 cut(s) 869, 962, 1639
HpyAV CCTTC 7 cut(s) 410, 469, 620, 622, 821, 1228, 1268
HpyCH4III ACNGT 6 cut(s) 550, 851, 1051, 1291, 1695, 2209
HpyCH4IV ACGT 2 cut(s) 912, 1800
HpyF10VI GCNNNNNNNGC 6 cut(s) 65, 477, 507, 1568, 1994, 2150
HpyF3I CTNAG 6 cut(s) 34, 149, 317, 1359, 1648, 1869
HpySE526I ACGT 2 cut(s) 912, 1800
Hsp92I GRCGYC 1 cut(s) 1448
KroI GCCGGC 1 cut(s) 678
KroNI GCCGGC 1 cut(s) 680
KspAI GTTAAC 1 cut(s) 1378
Kzo9I GATC 4 cut(s) 219, 1611, 1708, 1990
LmnI GCTCC 6 cut(s) 899, 1498, 1540, 1574, 1685, 2056
Lsp1109I GCAGC 9 cut(s) 46, 68, 71, 497, 615, 740, 996, 1177, 1530
LweI GCATC 4 cut(s) 715, 1443, 2095, 2188
MaeI CTAG 6 cut(s) 645, 1179, 1307, 1706, 1886, 2178
MaeII ACGT 2 cut(s) 912, 1800
MaeIII GTNAC 7 cut(s) 143, 252, 395, 550, 563, 1796, 2165
MalI GATC 4 cut(s) 221, 1613, 1710, 1992
MbiI CCGCTC 1 cut(s) 1358
MboI GATC 4 cut(s) 219, 1611, 1708, 1990
MboII GAAGA 9 cut(s) 22, 403, 635, 1114, 1235, 1322, 1564, 1642, 1955
MfeI CAATTG 2 cut(s) 705, 2170
MflI RGATCY 1 cut(s) 219
MhlI GDGCHC 2 cut(s) 1354, 1579
MlyI GAGTC 2 cut(s) 497, 1272
MmeI TCCRAC 3 cut(s) 255, 1054, 1476
Mph1103I ATGCAT 1 cut(s) 754
MroNI GCCGGC 1 cut(s) 678
MroXI GAANNNNTTC 1 cut(s) 925
MseI TTAA 6 cut(s) 243, 1011, 1377, 1977, 2009, 2243
MslI CAYNNNNRTG 4 cut(s) 583, 1413, 1623, 2051
MspA1I CMGCKG 1 cut(s) 59
MspI CCGG 2 cut(s) 363, 679
MspR9I CCNGG 4 cut(s) 81, 277, 819, 1864
MunI CAATTG 2 cut(s) 705, 2170
Mva1269I GAATGC 1 cut(s) 1017
MvaI CCWGG 4 cut(s) 81, 277, 819, 1864
MwoI GCNNNNNNNGC 6 cut(s) 65, 477, 507, 1568, 1994, 2150
NaeI GCCGGC 1 cut(s) 680
NcoI CCATGG 2 cut(s) 2052, 2090
NdeII GATC 4 cut(s) 219, 1611, 1708, 1990
NgoMIV GCCGGC 1 cut(s) 678
NlaIV GGNNCC 3 cut(s) 221, 367, 1758
NmuCI GTSAC 4 cut(s) 395, 550, 1796, 2165
NsiI ATGCAT 1 cut(s) 754
NspI RCATGY 3 cut(s) 756, 774, 2224
NspV TTCGAA 1 cut(s) 414
PaeI GCATGC 2 cut(s) 756, 2224
PaeR7I CTCGAG 1 cut(s) 1229
PceI AGGCCT 2 cut(s) 1867, 2032
PciI ACATGT 1 cut(s) 770
PcsI WCGNNNNNNNCGW 1 cut(s) 411
PctI GAATGC 1 cut(s) 1017
PdiI GCCGGC 1 cut(s) 680
PdmI GAANNNNTTC 1 cut(s) 925
PfeI GAWTC 4 cut(s) 1523, 1699, 1927, 2226
PflFI GACNNNGTC 2 cut(s) 1492, 1949
PleI GAGTC 2 cut(s) 496, 1271
PpsI GAGTC 2 cut(s) 496, 1271
PpuMI RGGWCCY 1 cut(s) 1190
PscI ACATGT 1 cut(s) 770
PshAI GACNNNNGTC 1 cut(s) 852
Psp5II RGGWCCY 1 cut(s) 1190
Psp6I CCWGG 4 cut(s) 79, 275, 817, 1862
PspGI CCWGG 4 cut(s) 79, 275, 817, 1862
PspN4I GGNNCC 3 cut(s) 221, 367, 1758
PspPI GGNCC 3 cut(s) 1190, 1756, 2111
PspPPI RGGWCCY 1 cut(s) 1190
PstI CTGCAG 5 cut(s) 64, 605, 893, 1222, 1573
PstNI CAGNNNCTG 1 cut(s) 1568
PsuI RGATCY 1 cut(s) 219
PsyI GACNNNGTC 2 cut(s) 1492, 1949
PvuII CAGCTG 1 cut(s) 59
RsaI GTAC 9 cut(s) 440, 547, 915, 1295, 1644, 1751, 2182, 2188, 2213
RsaNI GTAC 9 cut(s) 439, 546, 914, 1294, 1643, 1750, 2181, 2187, 2212
RseI CAYNNNNRTG 4 cut(s) 583, 1413, 1623, 2051
SaqAI TTAA 6 cut(s) 243, 1011, 1377, 1977, 2009, 2243
Sau3AI GATC 4 cut(s) 219, 1611, 1708, 1990
Sau96I GGNCC 3 cut(s) 1190, 1756, 2111
ScaI AGTACT 1 cut(s) 2182
SchI GAGTC 2 cut(s) 497, 1272
ScrFI CCNGG 4 cut(s) 81, 277, 819, 1864
SduI GDGCHC 2 cut(s) 1354, 1579
SfaNI GCATC 4 cut(s) 715, 1443, 2095, 2188
SfcI CTRYAG 5 cut(s) 60, 601, 889, 1218, 1569
Sfr274I CTCGAG 1 cut(s) 1229
SfuI TTCGAA 1 cut(s) 414
SinI GGWCC 2 cut(s) 1190, 1756
SlaI CTCGAG 1 cut(s) 1229
SmiMI CAYNNNNRTG 4 cut(s) 583, 1413, 1623, 2051
SmlI CTYRAG 3 cut(s) 69, 335, 1229
SmoI CTYRAG 3 cut(s) 69, 335, 1229
SphI GCATGC 2 cut(s) 756, 2224
SseBI AGGCCT 2 cut(s) 1867, 2032
SsiI CCGC 3 cut(s) 1356, 1546, 1560
SspI AATATT 1 cut(s) 1966
SspMI CTAG 6 cut(s) 645, 1179, 1307, 1706, 1886, 2178
StuI AGGCCT 2 cut(s) 1867, 2032
StyD4I CCNGG 4 cut(s) 79, 275, 817, 1862
StyI CCWWGG 5 cut(s) 329, 472, 1267, 2052, 2090
TaaI ACNGT 6 cut(s) 550, 851, 1051, 1291, 1695, 2209
TaiI ACGT 2 cut(s) 915, 1803
TaqI TCGA 3 cut(s) 414, 1230, 2229
TatI WGTACW 4 cut(s) 438, 1293, 1642, 2180
TauI GCSGC 1 cut(s) 1562
TfiI GAWTC 4 cut(s) 1523, 1699, 1927, 2226
Tru1I TTAA 6 cut(s) 243, 1011, 1377, 1977, 2009, 2243
Tru9I TTAA 6 cut(s) 243, 1011, 1377, 1977, 2009, 2243
TscAI CASTG 5 cut(s) 898, 1296, 1584, 1914, 2128
TseFI GTSAC 4 cut(s) 395, 550, 1796, 2165
TseI GCWGC 9 cut(s) 56, 59, 84, 510, 603, 728, 984, 1165, 1543
Tsp45I GTSAC 4 cut(s) 395, 550, 1796, 2165
TspGWI ACGGA 2 cut(s) 975, 1802
TspRI CASTG 5 cut(s) 898, 1296, 1584, 1914, 2128
Tth111I GACNNNGTC 2 cut(s) 1492, 1949
VpaK11BI GGWCC 2 cut(s) 1190, 1756
XapI RAATTY 4 cut(s) 103, 158, 1209, 1932
XbaI TCTAGA 1 cut(s) 1705
XceI RCATGY 3 cut(s) 756, 774, 2224
XhoI CTCGAG 1 cut(s) 1229
XmnI GAANNNNTTC 1 cut(s) 925
XspI CTAG 6 cut(s) 645, 1179, 1307, 1706, 1886, 2178
ZrmI AGTACT 1 cut(s) 2182
Zsp2I ATGCAT 1 cut(s) 754
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.