Rw0G023100

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig01076
OG0000066
Physical Location & Seq
Forward (+)
3361 .. 7254
3894 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G023100.1

Sequence Viewer

Length: 2307 bp
ATGGCCATACGTGGGATGCTGATTATGCAACTCTCACCGGTCGGAGTATTGATTCTGGTCCTATTATTAGAAGCGTCAGCAGCAGCAGCAACTCAAGCCTTTCCACCTCAATCCAAGTCAGGCTGCACTGATCACTGTGGTAGTCTCACAATTCAATATCCCTTTGGCGTAGAGCCTGGTTGTTACAAGACAGATGCCTTTTCTGTCAGTTGTAACAAGTCCACTGAACCCCCAACATTAATGTGGGGGCGAACCATCTCTATTACTGGCATATCTCTGGCTAAGGGTGAGATGCAAATAATGCAGTTCATAGCCAAAGATTGCTATGACAGCCGGGGTGTGACTACTTCCAACGTCAGAGCTGACCTCCGGGTGCCATCTCCCTTCACCATATCTCATACAAAGAACAAGTTTGTTGCTGTTGGTTGTGATACTTATGCAGTTTTCAGAGGTTACCGTGCAGACGGAGAAAGATACACATCCGGGTGCATGTCCATATGTGACAATTTCGACAGCGTCGAGAAGTCTTGCTCAGGCATTGGATGTTGCCACACTGACATCCCTAGTGGACTCAAAAATCGTACTGTCACATTGGATAGCTATTCCAATCATACATCTGTACAGAGCTTCAACCCGTGCAGCTACGCCTTTATTGTGGAAGAAGGCAAGTTCACATTTTCCCCGAATACGAGTTTTAAGCAACTGGACAACACTACAGAGCTTCCGATGATTCTTAATTGGGAAATTGGGGAAGGGACTTGTGAGATAGCTACGAAGAGGGATGATTATGCCTGCAAGGCTAAGAGCAGTAAGTGTATCAATCGGACCATCAGCAATGAGCTGTCTCGTTACTTTTGCCAGTGCCTACCAGGTTATGAAGGAAACCCCTACCTTCCAGACGGTTGCCAAGATATTGATGAGTGCAAGGCTTCAAACTCCTGCACCAACGGGACATGTGTAAATTCACCCGGAGATTACTCCTGTTTGTGTAATGAAGGGTTCATACACGACGGTGTGAATAACAAAATTTGCACCCAGGACAATTCCAAAAATCAACCAAAGATCACACTCCTGCTTAAGATATCATTGGGTTTAAGTGGGGGCTTCTTGGTTTTACTCGTTGGAATTTTGTGGGTATGTTGGGGAATAAAGAAGAGACAATTCAGTAAACTCAAAGAAAAGTACTTCAAAGAGAATGGTGGCTTGTTGTTGCTACAACAACTTGCTAGTCATGGAAGCTCGTTGGAGACAACAAAAATCTTTACTGCTGAAGAACTAAAGAAGGCAACAAACAATTATCATGAAAGTAGAATCCTTGGAGAAGGAGGCTATGGAACAGTTTACAAAGGAATACTACCAGATGACAAAGTGGTGGCCATAAAGAAGTCAAAAGGTGGTGCCCCAACCCAAAGTGATCAGTTTGTTAACGAGGTGATTATTCTTTCTAAAATCAACCACAGAAATGTGGTGAAGCTATTAGGTTGTTGTTTAGAAACAGAAGTACCTTTATTAGTATACGAGTTCATCACCCATGGCACTCTTTATGAGCATATTCATAAAAAAAGATCACCACTCTCATTTGACTTAAGAATGAAGATAGCAGTTGAAACTGCAGAAGCATTAGCCCATTTGCATTCCTCAATTTCCACACCTATCATACATCGAGATGTGAAAGCAGCAAATATTTTGTTAGATGATAATTATACAGCGAAAGTGTCAGACTTTGGAGCTTCACGATTGGTTCCTTCAGGACAAACAGAAATACAAACGCTAGTTCTCGGGACATTCGGATACCTAGACCCTGAATATCTGCAATCAAACCAACTGACAGAAAAGAGTGATGTTTACAGCTTTGGAGTTGTCCTAGTGGAGCTACTAACAAGCAAAGTGCCTGTTTCTAAAGATAGATGCTTAGCAAGTATCTTTGTTGCTTCCATGGAAGAAGATTGGTTGAATCATATTCTTGATGATGATATAGTGAATGAGCGAAACATTGAGACCGTAAAGGAAGTGGCCAATCTTGCAAAAAGATGCTTGAGGGTAAAAGGAGAGGAAAGGCCTACCATGAAAGAAGTTGCTAAAGAGTTGGAGGAAATGAGTGTCATGGCAAAACATCCGTGGGGATTTAATGCTAATTTCTGCGGAGAAGAGAATGAGTACTTGCTTGGGTCACTTAATTCAGAAGCTTTTGTTGTGGGTGTTGGAGGAGGTGATTGCAGTTCTAGTGGTCTAACTAGTGGTACGACCAATGCATATGACAGCATGCAAAACCAACTGCAGTTGATGCCATACGGTGGTGTACGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK

Protein Analysis

768

Amino Acids

84.33

Weight (kDa)

5.89

Isoelectric Point (pI)

41.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 42 - 101 1.2e-08 Wall-associated receptor kinase galacturonan-binding
Pkinase PF00069 433 - 694 5.3e-51 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 436 - 696 1.8e-51 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21210 AT1G21230 AT1G21240 AT1G21240 AT1G21245 AT1G21250 AT1G21270 AT1G22720 AT3G25490
fragaria_vesca FvH4_2g35051 FvH4_2g35051 FvH4_2g35060 FvH4_3g10281 FvH4_3g10285 FvH4_3g10286 FvH4_3g10311 FvH4_3g10311 FvH4_3g10320 FvH4_3g10332 FvH4_3g10340 FvH4_3g10350 FvH4_3g10360 FvH4_3g10390 FvH4_3g10412 FvH4_3g10413 FvH4_3g10461 FvH4_3g10462 FvH4_3g10470 FvH4_3g10510 FvH4_3g10520 FvH4_3g10530 FvH4_3g10550 FvH4_3g10560 FvH4_3g10611 FvH4_3g11730 FvH4_6g53140 FvH4_6g53142 FvH4_7g31880 FvH4_7g31910 FvH4_7g31920
malus_domestica MD05G1269900.v1.1 MD05G1270000.v1.1 MD05G1270200.v1.1 MD05G1270400.v1.1 MD05G1270500.v1.1 MD05G1270600.v1.1 MD05G1270800.v1.1 MD05G1270900.v1.1 MD07G1183800.v1.1 MD08G1107600.v1.1 MD10G1248500.v1.1 MD10G1249200.v1.1 MD10G1249500.v1.1 MD10G1250000.v1.1 MD10G1250500.v1.1 MD10G1250900.v1.1 MD10G1251200.v1.1 MD10G1251400.v1.1 MD13G1004600.v1.1 MD15G1088400.v1.1
prunus_persica Prupe.1G442400_v2.0.a1 Prupe.1G442500_v2.0.a1 Prupe.2G223400_v2.0.a1 Prupe.4G091900_v2.0.a1 Prupe.4G093200_v2.0.a1 Prupe.4G093300_v2.0.a1 Prupe.4G093400_v2.0.a1 Prupe.4G093500_v2.0.a1 Prupe.4G094100_v2.0.a1 Prupe.4G094200_v2.0.a1 Prupe.4G094300_v2.0.a1 Prupe.6G358300_v2.0.a1
pyrus_communis pycom08g08990 pycom10g20660 pycom10g20670 pycom10g20680 pycom10g20710 pycom10g20740 pycom13g00400 pycom15g08290
rosa_chinensis RchiOBHm_Chr1g0326861 RchiOBHm_Chr1g0326871 RchiOBHm_Chr1g0331391 RchiOBHm_Chr1g0336911 RchiOBHm_Chr1g0364001 RchiOBHm_Chr2g0174541 RchiOBHm_Chr2g0175041 RchiOBHm_Chr2g0175101 RchiOBHm_Chr2g0175121 RchiOBHm_Chr3g0486331 RchiOBHm_Chr5g0015581 RchiOBHm_Chr5g0016551 RchiOBHm_Chr5g0016561 RchiOBHm_Chr5g0016581 RchiOBHm_Chr5g0016601 RchiOBHm_Chr5g0016611 RchiOBHm_Chr5g0016641 RchiOBHm_Chr5g0016661 RchiOBHm_Chr5g0016691 RchiOBHm_Chr5g0016701 RchiOBHm_Chr5g0016731 RchiOBHm_Chr5g0016741 RchiOBHm_Chr5g0016811 RchiOBHm_Chr5g0016841 RchiOBHm_Chr5g0016871 RchiOBHm_Chr5g0016881 RchiOBHm_Chr5g0016931 RchiOBHm_Chr5g0017021 RchiOBHm_Chr5g0017041 RchiOBHm_Chr5g0017071 RchiOBHm_Chr5g0017091 RchiOBHm_Chr5g0017121 RchiOBHm_Chr5g0017141 RchiOBHm_Chr5g0017291 RchiOBHm_Chr5g0017321 RchiOBHm_Chr5g0017401 RchiOBHm_Chr5g0019001 RchiOBHm_Chr5g0019071 RchiOBHm_Chr5g0028941 RchiOBHm_Chr5g0052141 RchiOBHm_Chr5g0054721 RchiOBHm_Chr5g0054801 RchiOBHm_Chr5g0054931 RchiOBHm_Chr5g0054941 RchiOBHm_Chr6g0259291 RchiOBHm_Chr6g0292991 RchiOBHm_Chr6g0306911
rosa_laevigata RLG00000009465 RLG00000009468 RLG00000010755 RLG00000011976 RLG00000014541 RLG00000014542 RLG00000015169 RLG00000022308 RLG00000027529 RLG00000029324 RLG00000030244 RLG00000030245 RLG00000032210 RLG00000032211 RLG00000032257 RLG00000032259 RLG00000032260 RLG00000032261 RLG00000032262 RLG00000032268 RLG00000032269 RLG00000032272 RLG00000032282 RLG00000032283 RLG00000032308 RLG00000032413 RLG00000033129 RLG00000034945
rosa_multiflora Rmu_co8241929.1_g000001 Rmu_co8269227.1_g000001 Rmu_co8461259.1_g000001 Rmu_co8495385.1_g000001 Rmu_sc0000028.1_g000003 Rmu_sc0000083.1_g000009 Rmu_sc0000083.1_g000010 Rmu_sc0000083.1_g000031 Rmu_sc0000083.1_g000043 Rmu_sc0000083.1_g000046 Rmu_sc0000083.1_g000047 Rmu_sc0000563.1_g000024 Rmu_sc0000842.1_g000012 Rmu_sc0001235.1_g000001 Rmu_sc0001235.1_g000005 Rmu_sc0001235.1_g000009 Rmu_sc0001235.1_g000015 Rmu_sc0001235.1_g000016 Rmu_sc0001235.1_g000017 Rmu_sc0001473.1_g000036 Rmu_sc0001607.1_g000016 Rmu_sc0001788.1_g000006 Rmu_sc0002018.1_g000008 Rmu_sc0002028.1_g000001 Rmu_sc0002680.1_g000011 Rmu_sc0002798.1_g000023 Rmu_sc0002886.1_g000023 Rmu_sc0002989.1_g000012 Rmu_sc0003064.1_g000009 Rmu_sc0003382.1_g000006 Rmu_sc0003630.1_g000002 Rmu_sc0003630.1_g000047 Rmu_sc0003630.1_g000066 Rmu_sc0003630.1_g000067 Rmu_sc0003649.1_g000010 Rmu_sc0003688.1_g000005 Rmu_sc0003920.1_g000007 Rmu_sc0004765.1_g000007 Rmu_sc0004765.1_g000008 Rmu_sc0004920.1_g000011 Rmu_sc0005223.1_g000008 Rmu_sc0005223.1_g000010 Rmu_sc0005223.1_g000015 Rmu_sc0005223.1_g000016 Rmu_sc0005319.1_g000014 Rmu_sc0005319.1_g000015 Rmu_sc0005319.1_g000018 Rmu_sc0005319.1_g000019 Rmu_sc0005319.1_g000025 Rmu_sc0005418.1_g000007 Rmu_sc0005418.1_g000012 Rmu_sc0006106.1_g000011 Rmu_sc0006914.1_g000012 Rmu_sc0007023.1_g000001 Rmu_sc0007662.1_g000001 Rmu_sc0007920.1_g000002 Rmu_sc0007920.1_g000013 Rmu_sc0008053.1_g000016 Rmu_sc0008053.1_g000017 Rmu_sc0008053.1_g000018 Rmu_sc0008053.1_g000023 Rmu_sc0008053.1_g000050 Rmu_sc0009042.1_g000007 Rmu_sc0009042.1_g000008 Rmu_sc0010642.1_g000008 Rmu_sc0011109.1_g000002 Rmu_sc0011314.1_g000006 Rmu_sc0011974.1_g000006 Rmu_sc0012179.1_g000009 Rmu_sc0015169.1_g000001 Rmu_sc0016368.1_g000002 Rmu_sc0022659.1_g000001 Rmu_sc0027371.1_g000001 Rmu_sc0028213.1_g000001 Rmu_sc0034526.1_g000001 Rmu_sc0039366.1_g000002 Rmu_ssc0000050.1_g000084 Rmu_ssc0000050.1_g000088 Rmu_ssc0000167.1_g000017
rosa_roxburghii Rroxscaffold_1G00011340 Rroxscaffold_1G00025710 Rroxscaffold_1G00025870 Rroxscaffold_1G00026070 Rroxscaffold_1G00026170 Rroxscaffold_1G00026200 Rroxscaffold_1G00026270 Rroxscaffold_1G00050950 Rroxscaffold_1G00058900 Rroxscaffold_1G00060040 Rroxscaffold_1G00060060 Rroxscaffold_1G00060070 Rroxscaffold_1G00060080 Rroxscaffold_1G00060110 Rroxscaffold_1G00060120 Rroxscaffold_1G00060140 Rroxscaffold_1G00060170 Rroxscaffold_1G00060210 Rroxscaffold_1G00060220 Rroxscaffold_1G00060240 Rroxscaffold_1G00060250 Rroxscaffold_1G00060260 Rroxscaffold_1G00060300 Rroxscaffold_1G00060320 Rroxscaffold_1G00060330 Rroxscaffold_1G00060900 Rroxscaffold_1G00060920 Rroxscaffold_1G00073980 Rroxscaffold_2G00077560 Rroxscaffold_2G00077580 Rroxscaffold_2G00146990 Rroxscaffold_3G00236200 Rroxscaffold_3G00236210 Rroxscaffold_3G00236220 Rroxscaffold_4G00292760 Rroxscaffold_4G00315200 Rroxscaffold_4G00315240 Rroxscaffold_4G00326240 Rroxscaffold_4G00328980 Rroxscaffold_5G00341890 Rroxscaffold_7G00161350 Rroxscaffold_7G00174490 Rroxscaffold_7G00206550
rosa_rugosa Rorug01G0093000 Rorug01G0093100 Rorug01G0128800 Rorug01G0312000 Rorug01G0312000 Rorug02G0581100 Rorug03G0224600 Rorug05G0028500 Rorug05G0032700 Rorug05G0032800 Rorug05G0032900 Rorug05G0032900 Rorug05G0033000 Rorug05G0033100 Rorug05G0033300 Rorug05G0033400 Rorug05G0033900 Rorug05G0034900 Rorug05G0035000 Rorug05G0046900 Rorug05G0047500 Rorug05G0047500 Rorug05G0111600 Rorug05G0287900 Rorug05G0288700 Rorug05G0586900 Rorug06G0231700 Rorug06G0354300 Rorug06G0354400
rosa_samantha Rh1DG122200 Rh2DG686800 Rh5BG136600 Rh6AG344500 Rh6BG351900 Rh6CG359000 Rh6CG359100 Rh6CG387600 Rh6CG480500 Rh6DG344600 Rh6DG467400
rosa_wichuraiana Rw0G010250 Rw0G014820 Rw0G021400 Rw0G023100 Rw1G009450 Rw1G012100 Rw1G012180 Rw1G028360 Rw2G054260 Rw3G024370 Rw3G024420 Rw3G024470 Rw5G010850 Rw5G011010 Rw5G011020 Rw5G011050 Rw5G011080 Rw5G011120 Rw5G012300 Rw5G018470 Rw5G033830 Rw6G008930 Rw6G030070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 373, 1397
AccB7I CCANNNNNTGG 1 cut(s) 2294
AccI GTMKAC 1 cut(s) 1515
AciI CCGC 1 cut(s) 2142
AcoI YGGCCR 3 cut(s) 3, 1374, 2013
AcsI RAATTY 3 cut(s) 961, 1026, 1125
AcuI CTGAAG 2 cut(s) 1290, 1731
AfaI GTAC 7 cut(s) 583, 621, 1184, 1503, 2159, 2242, 2301
AfiI CCNNNNNNNGG 2 cut(s) 12, 2294
AflII CTTAAG 2 cut(s) 1076, 1585
AflIII ACRYGT 1 cut(s) 953
AgeI ACCGGT 1 cut(s) 37
AgsI TTSAA 6 cut(s) 155, 631, 933, 1189, 1607, 1954
AhlI ACTAGT 1 cut(s) 2234
AjnI CCWGG 3 cut(s) 175, 868, 1035
AleI CACNNNNGTG 1 cut(s) 1011
Alw26I GTCTC 5 cut(s) 149, 849, 1150, 1241, 1991
Ama87I CYCGRG 1 cut(s) 1778
AoxI GGCC 4 cut(s) 3, 1374, 2013, 2057
ApeKI GCWGC 6 cut(s) 80, 83, 86, 123, 639, 1676
ApoI RAATTY 3 cut(s) 961, 1026, 1125
AseI ATTAAT 1 cut(s) 239
AsiGI ACCGGT 1 cut(s) 37
AspS9I GGNCC 2 cut(s) 58, 825
AsuC2I CCSGG 4 cut(s) 335, 371, 484, 969
AsuHPI GGTGA 9 cut(s) 27, 299, 379, 957, 1444, 1480, 1519, 1560, 2222
AvaI CYCGRG 1 cut(s) 1778
AvaII GGWCC 2 cut(s) 58, 825
BaeGI GKGCMC 1 cut(s) 1402
BaeI ACNNNNGTAYC 2 cut(s) 1485, 1518
BalI TGGCCA 3 cut(s) 5, 1376, 2015
BanI GGYRCC 2 cut(s) 373, 1397
BarI GAAGNNNNNNTAC 2 cut(s) 987, 1019
BbvI GCAGC 6 cut(s) 92, 95, 98, 110, 651, 1688
BccI CCATC 3 cut(s) 263, 385, 836
BciT130I CCWGG 3 cut(s) 177, 870, 1037
BciVI GTATCC 1 cut(s) 1784
BclI TGATCA 2 cut(s) 130, 1414
BcnI CCSGG 4 cut(s) 335, 371, 484, 969
BcoDI GTCTC 5 cut(s) 149, 849, 1150, 1241, 1991
BcuI ACTAGT 1 cut(s) 2234
BfaI CTAG 7 cut(s) 564, 1227, 1772, 1796, 1865, 2223, 2235
BfmI CTRYAG 3 cut(s) 714, 1611, 2276
BfrI CTTAAG 2 cut(s) 1076, 1585
BfuI GTATCC 1 cut(s) 1784
BglI GCCNNNNNGGC 1 cut(s) 797
BisI GCNGC 6 cut(s) 81, 84, 87, 124, 640, 1677
BlpI GCTNAGC 1 cut(s) 1912
BlsI GCNGC 6 cut(s) 82, 85, 88, 125, 641, 1678
BmcAI AGTACT 2 cut(s) 1184, 2159
Bme1390I CCNGG 7 cut(s) 177, 335, 371, 484, 870, 969, 1037
Bme18I GGWCC 2 cut(s) 58, 825
BmeT110I CYCGRG 1 cut(s) 1778
BmgT120I GGNCC 2 cut(s) 58, 825
BmiI GGNNCC 3 cut(s) 375, 1399, 1743
BmrFI CCNGG 7 cut(s) 177, 335, 371, 484, 870, 969, 1037
BmsI GCATC 6 cut(s) 6, 184, 282, 1898, 2021, 2274
BplI GAGNNNNNCTC 2 cut(s) 351, 383
Bpu10I CCTNAGC 2 cut(s) 282, 532
Bpu1102I GCTNAGC 1 cut(s) 1912
BpuEI CTTGAG 2 cut(s) 78, 2056
BpuMI CCSGG 4 cut(s) 335, 371, 484, 969
BsaAI YACGTR 1 cut(s) 11
BsaBI GATNNNNATC 1 cut(s) 478
BsaI GGTCTC 1 cut(s) 1991
BsaJI CCNNGG 6 cut(s) 334, 1035, 1315, 1531, 1935, 2117
BsaWI WCCGGW 1 cut(s) 37
Bsc4I CCNNNNNNNGG 2 cut(s) 12, 2294
Bse118I RCCGGY 1 cut(s) 37
Bse1I ACTGG 3 cut(s) 271, 708, 859
Bse3DI GCAATG 1 cut(s) 841
Bse8I GATNNNNATC 1 cut(s) 478
BseBI CCWGG 3 cut(s) 177, 870, 1037
BseDI CCNNGG 6 cut(s) 334, 1035, 1315, 1531, 1935, 2117
BseGI GGATG 6 cut(s) 21, 479, 548, 558, 787, 2113
BseJI GATNNNNATC 1 cut(s) 478
BseLI CCNNNNNNNGG 2 cut(s) 12, 2294
BseMI GCAATG 1 cut(s) 841
BseMII CTCAG 1 cut(s) 546
BseNI ACTGG 3 cut(s) 271, 708, 859
BseRI GAGGAG 1 cut(s) 2220
BseSI GKGCMC 1 cut(s) 1402
BseXI GCAGC 6 cut(s) 92, 95, 98, 110, 651, 1688
BsgI GTGCAG 4 cut(s) 109, 480, 658, 925
Bsh1285I CGRYCG 1 cut(s) 42
BshFI GGCC 4 cut(s) 5, 1376, 2015, 2059
BshNI GGYRCC 2 cut(s) 373, 1397
BshTI ACCGGT 1 cut(s) 37
BsiEI CGRYCG 1 cut(s) 42
BsiHKCI CYCGRG 1 cut(s) 1778
BsiSI CCGG 5 cut(s) 38, 334, 370, 483, 969
BslFI GGGAC 3 cut(s) 769, 964, 1795
BslI CCNNNNNNNGG 2 cut(s) 12, 2294
BsmAI GTCTC 5 cut(s) 149, 849, 1150, 1241, 1991
BsmFI GGGAC 3 cut(s) 769, 964, 1795
BsmI GAATGC 1 cut(s) 1633
BsnI GGCC 4 cut(s) 5, 1376, 2015, 2059
Bso31I GGTCTC 1 cut(s) 1991
BsoBI CYCGRG 1 cut(s) 1778
Bsp1286I GDGCHC 1 cut(s) 1402
Bsp1407I TGTACA 1 cut(s) 619
Bsp143I GATC 4 cut(s) 130, 1062, 1414, 1565
Bsp1720I GCTNAGC 1 cut(s) 1912
Bsp19I CCATGG 2 cut(s) 1531, 1935
BspACI CCGC 1 cut(s) 2142
BspANI GGCC 4 cut(s) 5, 1376, 2015, 2059
BspCNI CTCAG 1 cut(s) 545
BspHI TCATGA 1 cut(s) 1300
BspLI GGNNCC 3 cut(s) 375, 1399, 1743
BspMAI CTGCAG 2 cut(s) 1615, 2280
BspT107I GGYRCC 2 cut(s) 373, 1397
BspTI CTTAAG 2 cut(s) 1076, 1585
BspTNI GGTCTC 1 cut(s) 1991
BsrDI GCAATG 1 cut(s) 841
BsrFI RCCGGY 1 cut(s) 37
BsrGI TGTACA 1 cut(s) 619
BsrI ACTGG 3 cut(s) 271, 708, 859
BssAI RCCGGY 1 cut(s) 37
BssECI CCNNGG 6 cut(s) 334, 1035, 1315, 1531, 1935, 2117
BssMI GATC 4 cut(s) 130, 1062, 1414, 1565
BssNAI GTATAC 1 cut(s) 1516
BssT1I CCWWGG 3 cut(s) 1315, 1531, 1935
Bst1107I GTATAC 1 cut(s) 1516
Bst2UI CCWGG 3 cut(s) 177, 870, 1037
Bst4CI ACNGT 9 cut(s) 137, 458, 586, 902, 1013, 1339, 2002, 2294, 2304
Bst6I CTCTTC 3 cut(s) 770, 1148, 2142
BstAFI CTTAAG 2 cut(s) 1076, 1585
BstAPI GCANNNNNTGC 2 cut(s) 301, 2284
BstAUI TGTACA 1 cut(s) 619
BstBAI YACGTR 1 cut(s) 11
BstC8I GCNNGC 2 cut(s) 793, 2264
BstDEI CTNAG 4 cut(s) 282, 532, 801, 1912
BstDSI CCRYGG 3 cut(s) 1531, 1935, 2117
BstEII GGTNACC 1 cut(s) 452
BstF5I GGATG 6 cut(s) 21, 479, 548, 558, 787, 2113
BstKTI GATC 4 cut(s) 133, 1065, 1417, 1568
BstMAI GTCTC 5 cut(s) 149, 849, 1150, 1241, 1991
BstMBI GATC 4 cut(s) 130, 1062, 1414, 1565
BstMCI CGRYCG 1 cut(s) 42
BstMWI GCNNNNNNNGC 9 cut(s) 25, 80, 86, 95, 301, 330, 797, 2021, 2284
BstNI CCWGG 3 cut(s) 177, 870, 1037
BstNSI RCATGY 3 cut(s) 493, 957, 2266
BstPI GGTNACC 1 cut(s) 452
BstSCI CCNGG 7 cut(s) 175, 333, 369, 482, 868, 967, 1035
BstSFI CTRYAG 3 cut(s) 714, 1611, 2276
BstSLI GKGCMC 1 cut(s) 1402
BstV1I GCAGC 6 cut(s) 92, 95, 98, 110, 651, 1688
BstZ17I GTATAC 1 cut(s) 1516
BsuI GTATCC 1 cut(s) 1784
BsuRI GGCC 4 cut(s) 5, 1376, 2015, 2059
BtgI CCRYGG 3 cut(s) 1531, 1935, 2117
BtsCI GGATG 6 cut(s) 21, 479, 548, 558, 787, 2113
BtsIMutI CAGTG 5 cut(s) 126, 133, 222, 552, 866
Cac8I GCNNGC 2 cut(s) 793, 2264
CciI TCATGA 1 cut(s) 1300
Cfr10I RCCGGY 1 cut(s) 37
Cfr13I GGNCC 2 cut(s) 58, 825
CseI GACGC 2 cut(s) 63, 505
CsiI ACCWGGT 1 cut(s) 868
Csp6I GTAC 7 cut(s) 582, 620, 1183, 1502, 2158, 2241, 2300
CspAI ACCGGT 1 cut(s) 37
CviAII CATG 9 cut(s) 490, 954, 1232, 1301, 1532, 1936, 2065, 2104, 2263
CviQI GTAC 7 cut(s) 582, 620, 1183, 1502, 2158, 2241, 2300
DdeI CTNAG 4 cut(s) 282, 532, 801, 1912
DpnI GATC 4 cut(s) 132, 1064, 1416, 1567
DpnII GATC 4 cut(s) 130, 1062, 1414, 1565
EaeI YGGCCR 3 cut(s) 3, 1374, 2013
Eam1104I CTCTTC 3 cut(s) 770, 1148, 2142
EarI CTCTTC 3 cut(s) 770, 1148, 2142
Eco130I CCWWGG 3 cut(s) 1315, 1531, 1935
Eco147I AGGCCT 1 cut(s) 2059
Eco31I GGTCTC 1 cut(s) 1991
Eco32I GATATC 1 cut(s) 1083
Eco47I GGWCC 2 cut(s) 58, 825
Eco57I CTGAAG 2 cut(s) 1290, 1731
Eco88I CYCGRG 1 cut(s) 1778
Eco91I GGTNACC 1 cut(s) 452
EcoO65I GGTNACC 1 cut(s) 452
EcoRII CCWGG 3 cut(s) 175, 868, 1035
EcoRV GATATC 1 cut(s) 1083
EcoT14I CCWWGG 3 cut(s) 1315, 1531, 1935
EcoT22I ATGCAT 1 cut(s) 2254
ErhI CCWWGG 3 cut(s) 1315, 1531, 1935
FaeI CATG 9 cut(s) 493, 957, 1235, 1304, 1535, 1939, 2068, 2107, 2266
FaqI GGGAC 3 cut(s) 769, 964, 1795
FatI CATG 9 cut(s) 489, 953, 1231, 1300, 1531, 1935, 2064, 2103, 2262
FauNDI CATATG 2 cut(s) 497, 2254
FbaI TGATCA 2 cut(s) 130, 1414
FblI GTMKAC 1 cut(s) 1515
Fnu4HI GCNGC 6 cut(s) 81, 84, 87, 124, 640, 1677
FokI GGATG 6 cut(s) 28, 466, 545, 555, 794, 2100
Fsp4HI GCNGC 6 cut(s) 81, 84, 87, 124, 640, 1677
FspBI CTAG 7 cut(s) 564, 1227, 1772, 1796, 1865, 2223, 2235
GluI GCNGC 6 cut(s) 81, 84, 87, 124, 640, 1677
HaeIII GGCC 4 cut(s) 5, 1376, 2015, 2059
HapII CCGG 5 cut(s) 38, 334, 370, 483, 969
HgaI GACGC 2 cut(s) 63, 505
Hin1II CATG 9 cut(s) 493, 957, 1235, 1304, 1535, 1939, 2068, 2107, 2266
HincII GTYRAC 1 cut(s) 1426
HindII GTYRAC 1 cut(s) 1426
HindIII AAGCTT 1 cut(s) 2184
HinfI GANTC 5 cut(s) 52, 570, 730, 1311, 1954
HpaI GTTAAC 1 cut(s) 1426
HpaII CCGG 5 cut(s) 38, 334, 370, 483, 969
HphI GGTGA 9 cut(s) 27, 299, 379, 957, 1444, 1480, 1519, 1560, 2222
Hpy166II GTNNAC 9 cut(s) 222, 569, 672, 1169, 1342, 1426, 1516, 1846, 2300
Hpy188I TCNGA 8 cut(s) 44, 359, 449, 726, 825, 1720, 1790, 2182
Hpy188III TCNNGA 8 cut(s) 520, 896, 1301, 1664, 1734, 1749, 1780, 1964
Hpy8I GTNNAC 9 cut(s) 222, 569, 672, 1169, 1342, 1426, 1516, 1846, 2300
Hpy99I CGWCG 2 cut(s) 521, 1013
HpyAV CCTTC 9 cut(s) 394, 656, 746, 872, 902, 989, 1276, 1316, 1755
HpyCH4III ACNGT 9 cut(s) 137, 458, 586, 902, 1013, 1339, 2002, 2294, 2304
HpyCH4IV ACGT 2 cut(s) 10, 354
HpyF10VI GCNNNNNNNGC 9 cut(s) 25, 80, 86, 95, 301, 330, 797, 2021, 2284
HpyF3I CTNAG 4 cut(s) 282, 532, 801, 1912
HpySE526I ACGT 2 cut(s) 10, 354
Hsp92II CATG 9 cut(s) 493, 957, 1235, 1304, 1535, 1939, 2068, 2107, 2266
Ksp22I TGATCA 2 cut(s) 130, 1414
KspAI GTTAAC 1 cut(s) 1426
Kzo9I GATC 4 cut(s) 130, 1062, 1414, 1565
LmnI GCTCC 2 cut(s) 1727, 1870
Lsp1109I GCAGC 6 cut(s) 92, 95, 98, 110, 651, 1688
LweI GCATC 6 cut(s) 6, 184, 282, 1898, 2021, 2274
MabI ACCWGGT 1 cut(s) 868
MaeI CTAG 7 cut(s) 564, 1227, 1772, 1796, 1865, 2223, 2235
MaeII ACGT 2 cut(s) 10, 354
MaeIII GTNAC 8 cut(s) 182, 212, 340, 452, 500, 586, 848, 2169
MalI GATC 4 cut(s) 132, 1064, 1416, 1567
MboI GATC 4 cut(s) 130, 1062, 1414, 1565
MboII GAAGA 8 cut(s) 671, 787, 1165, 1283, 1606, 1952, 1955, 2159
MhlI GDGCHC 1 cut(s) 1402
MlsI TGGCCA 3 cut(s) 5, 1376, 2015
MluNI TGGCCA 3 cut(s) 5, 1376, 2015
MlyI GAGTC 1 cut(s) 564
MmeI TCCRAC 6 cut(s) 22, 375, 1102, 1224, 2067, 2182
Mox20I TGGCCA 3 cut(s) 5, 1376, 2015
Mph1103I ATGCAT 1 cut(s) 2254
MscI TGGCCA 3 cut(s) 5, 1376, 2015
MseI TTAA 9 cut(s) 239, 696, 735, 1077, 1094, 1425, 1586, 2127, 2175
MslI CAYNNNNRTG 5 cut(s) 241, 484, 1011, 1461, 1665
Msp20I TGGCCA 3 cut(s) 5, 1376, 2015
MspCI CTTAAG 2 cut(s) 1076, 1585
MspI CCGG 5 cut(s) 38, 334, 370, 483, 969
MspR9I CCNGG 7 cut(s) 177, 335, 371, 484, 870, 969, 1037
Mva1269I GAATGC 1 cut(s) 1633
MvaI CCWGG 3 cut(s) 177, 870, 1037
MwoI GCNNNNNNNGC 9 cut(s) 25, 80, 86, 95, 301, 330, 797, 2021, 2284
NciI CCSGG 4 cut(s) 335, 371, 484, 969
NcoI CCATGG 2 cut(s) 1531, 1935
NdeI CATATG 2 cut(s) 497, 2254
NdeII GATC 4 cut(s) 130, 1062, 1414, 1565
NlaIII CATG 9 cut(s) 493, 957, 1235, 1304, 1535, 1939, 2068, 2107, 2266
NlaIV GGNNCC 3 cut(s) 375, 1399, 1743
NmuCI GTSAC 4 cut(s) 340, 500, 586, 2169
NsiI ATGCAT 1 cut(s) 2254
NspI RCATGY 3 cut(s) 493, 957, 2266
OliI CACNNNNGTG 1 cut(s) 1011
PaeI GCATGC 1 cut(s) 2266
PagI TCATGA 1 cut(s) 1300
PceI AGGCCT 1 cut(s) 2059
PciI ACATGT 1 cut(s) 953
PcsI WCGNNNNNNNCGW 1 cut(s) 516
PctI GAATGC 1 cut(s) 1633
PfeI GAWTC 4 cut(s) 52, 730, 1311, 1954
PflFI GACNNNGTC 1 cut(s) 515
PflMI CCANNNNNTGG 1 cut(s) 2294
PinAI ACCGGT 1 cut(s) 37
PkrI GCNGC 6 cut(s) 82, 85, 88, 125, 641, 1678
PleI GAGTC 1 cut(s) 564
PpsI GAGTC 1 cut(s) 564
Ppu21I YACGTR 1 cut(s) 11
PscI ACATGT 1 cut(s) 953
PshBI ATTAAT 1 cut(s) 239
Psp6I CCWGG 3 cut(s) 175, 868, 1035
PspEI GGTNACC 1 cut(s) 452
PspGI CCWGG 3 cut(s) 175, 868, 1035
PspN4I GGNNCC 3 cut(s) 375, 1399, 1743
PspPI GGNCC 2 cut(s) 58, 825
PstI CTGCAG 2 cut(s) 1615, 2280
PsyI GACNNNGTC 1 cut(s) 515
RsaI GTAC 7 cut(s) 583, 621, 1184, 1503, 2159, 2242, 2301
RsaNI GTAC 7 cut(s) 582, 620, 1183, 1502, 2158, 2241, 2300
RseI CAYNNNNRTG 5 cut(s) 241, 484, 1011, 1461, 1665
SaqAI TTAA 9 cut(s) 239, 696, 735, 1077, 1094, 1425, 1586, 2127, 2175
SatI GCNGC 6 cut(s) 81, 84, 87, 124, 640, 1677
Sau3AI GATC 4 cut(s) 130, 1062, 1414, 1565
Sau96I GGNCC 2 cut(s) 58, 825
ScaI AGTACT 2 cut(s) 1184, 2159
SchI GAGTC 1 cut(s) 564
ScrFI CCNGG 7 cut(s) 177, 335, 371, 484, 870, 969, 1037
SduI GDGCHC 1 cut(s) 1402
SexAI ACCWGGT 1 cut(s) 868
SfaNI GCATC 6 cut(s) 6, 184, 282, 1898, 2021, 2274
SfcI CTRYAG 3 cut(s) 714, 1611, 2276
SinI GGWCC 2 cut(s) 58, 825
SmiMI CAYNNNNRTG 5 cut(s) 241, 484, 1011, 1461, 1665
SmlI CTYRAG 4 cut(s) 93, 1076, 1585, 2035
SmoI CTYRAG 4 cut(s) 93, 1076, 1585, 2035
SpeI ACTAGT 1 cut(s) 2234
SphI GCATGC 1 cut(s) 2266
SseBI AGGCCT 1 cut(s) 2059
SsiI CCGC 1 cut(s) 2142
SspI AATATT 1 cut(s) 1684
SspMI CTAG 7 cut(s) 564, 1227, 1772, 1796, 1865, 2223, 2235
StuI AGGCCT 1 cut(s) 2059
StyD4I CCNGG 7 cut(s) 175, 333, 369, 482, 868, 967, 1035
StyI CCWWGG 3 cut(s) 1315, 1531, 1935
TaaI ACNGT 9 cut(s) 137, 458, 586, 902, 1013, 1339, 2002, 2294, 2304
TaiI ACGT 2 cut(s) 13, 357
TaqI TCGA 3 cut(s) 510, 519, 1663
TatI WGTACW 3 cut(s) 619, 1182, 2157
TfiI GAWTC 4 cut(s) 52, 730, 1311, 1954
Tru1I TTAA 9 cut(s) 239, 696, 735, 1077, 1094, 1425, 1586, 2127, 2175
Tru9I TTAA 9 cut(s) 239, 696, 735, 1077, 1094, 1425, 1586, 2127, 2175
TscAI CASTG 5 cut(s) 133, 140, 229, 559, 866
TseFI GTSAC 4 cut(s) 340, 500, 586, 2169
TseI GCWGC 6 cut(s) 80, 83, 86, 123, 639, 1676
Tsp45I GTSAC 4 cut(s) 340, 500, 586, 2169
TspDTI ATGAA 9 cut(s) 298, 891, 991, 1008, 1317, 1513, 1544, 1607, 2081
TspGWI ACGGA 2 cut(s) 480, 2106
TspRI CASTG 5 cut(s) 133, 140, 229, 559, 866
Tth111I GACNNNGTC 1 cut(s) 515
Van91I CCANNNNNTGG 1 cut(s) 2294
Vha464I CTTAAG 2 cut(s) 1076, 1585
VpaK11BI GGWCC 2 cut(s) 58, 825
VspI ATTAAT 1 cut(s) 239
XapI RAATTY 3 cut(s) 961, 1026, 1125
XceI RCATGY 3 cut(s) 493, 957, 2266
XcmI CCANNNNNNNNNTGG 1 cut(s) 240
XmiI GTMKAC 1 cut(s) 1515
XspI CTAG 7 cut(s) 564, 1227, 1772, 1796, 1865, 2223, 2235
ZrmI AGTACT 2 cut(s) 1184, 2159
Zsp2I ATGCAT 1 cut(s) 2254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.