Rh6CG480500

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0000066
Physical Location & Seq
Reverse (-)
64743601 .. 64747022
3422 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG480500.1

Sequence Viewer

Length: 2253 bp
ATGTCTATGAGGATGAAGAAAATGTTTTTGCTGCAATTGGTGCTGGTTGCAATAGAGGTGCTACTAGTATCATCCCAAACAGTGGATAGTACGAGAGAGGGTTGCCAAGACAAATGTGGCAATGTTAGCATTCCCTACCCATTCGGAACAAGAGAGGGTTGTTACCATGATGCGGATTTCCTCATTACTTGTAATAGCACAAGTTATAACCCTCCGAAGCCATTTCTGAGAAAGGGTAACATTGAGGTCACAAACATATCCATTGATGGCAAGTTGAATATCATGCAATACATAGCTCAAGATTGCTACGATTCATCTGGTTTCCGTATCAAGAAAAATTCTGCATCGACCCGCCTTTCAAAATTCATCATCTCCAACACCGATAATAAGTTCATTGCAGTTGGATGTGACACTTATGCAGGAATCCGAGGCTTCCAGGGCAAAATCATGAAGTACACAGGTGGCTGCATATCAACATGTGACAGCATTGATTATGTTGCCAATGATTCATGTTCCGGTATAGGGTGTTGCCAGACATCAATTGCTAAAGGTGTGAGCCATTTTGATGTGTTTTTGCACAGTTACCAGAATCATACAGATGTTTGGTCTTTCAATCCATGCAGCTTCGCCTTTATTGTGGAAGAAAGCAAGTTCAACTTCTCCAGAGCTATGCTCATGGATTTAAAGGATGTAACAGTACTTCCTGCTGTGCTTGATTGGTCAATTGGAAATGAGACTTGCAAGCAAGTTGAAGGCAAAATGATAGCTGGCAGCGCGTGCCAGGGGAACAGCACATGTGTTGATGTCGAAAATGGGTCTGGATACCGATGCAAGTGCAAGGATGGTTATCAAGGGAACCCATACCTCTCTAATGGTTGTCAAGATATTGATGAATGTGAGGATCCAAAACTCAGTAACTGTGCACAAAAATGTGTAAATACAGAGGGAAGTCAATACTGTTCTTGCCGCAAAGGGTACCATGGCAATGGTATAGCAGGGGAAGAAGGTTGCATAGCTAACAGAGCATTAGTTGTTCAAATCACTGTTGGAATTGCTGTCGGCCTCATATCGTTGCTAACGTGTTGCTCTTGGTTATATCTCGGCTACAGAAGATGGAAACTCATGAAGCTGAAGGAGAGATTCTTCAGACAAAATGGAGGATTAATGTTACAACAACAACTTTCAGAACGACAAGGTGGTGCCACTAACCAAAATGCAAAAATCTTTACTGCAGAAGAACTTGAGAAGGCCACCAACAACTACCATGAGACTAGAATCGTTGGCAAAGGAGGCTACGGTACAGTGTACAAAGGAATTTTATCAGATGGAAGAGTAGTTGCAATCAAGAAATCCAAAATAGTTGATCAAAGCCAAATAGAGCAATTCATTAATGAAGTACTGGTGCTTTCCCAAATCAATCACAGGAATGTGGTGAAGCTTTTGGGTTGTTGTTTCGAGACAGAAGTCCCACGACTAGTTTATGAGTTTGTCACCAGTGGTACGCTATTTGACTACATCCACAACAAAAGCAAGGCATCCAACTTTGCTTGGGAAACTCGTTTAAGGATAGCTGCTGGAGTGCTATCATACTTGCACTCAGCTGCTTCTGTCCCTATTATCCATAGAGATGTCAAGTCCACAAATATACTTTTAGATGAAACCCTTACAGCAAAAGTGTCTGATTTTGGTGCCTCCAGATTGGTACCATTGGATCAAACTGAGTTATCGACAATGGTACAAGGCACGCTCGGGTACTTAGACCCTGAATACTTTCAAACAAGCCAATTGACTGACAAGAGTGATGTCTATAGCTTTGGGGTTGTCCTTGTGGAGCTACTAACAGGAAAGAAGGCGCTTTCGTTCGACAGGCAAGAGGAGGATAGAAATCTAGCAATGTATTTTCTTTCCGCACTGAAAGAAAACCGCTTGGTACAAGTGGTTGATGAGTGTGTTCTGAATGGGGCAAATGACGAGCAGCTTAAGGAAGTTTCTAACCTTGCAAAGAGGTGTTTGAAAGTGAAAGGAGAAGAAAGACCAACCATGATGGAAGTAGCAAGCGAGCTTGAGGGACTAAGAAGGATGGCAATGCATCATCCCTGGGTTAATAATGATGAATCCAATGCACAGGAGAATGAAAGCTTGCTTGGTGAAATATCACTGGAAACGTTTAGTCATGGAGGTGGTGGCGATACGAGTTCTGGGTATGATACAATGAGGAACCACATCATATTACCAGTAAATGATGGGAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF_CA GUB_WAK_bind Pkinase WAK

Protein Analysis

750

Amino Acids

83.18

Weight (kDa)

6.03

Isoelectric Point (pI)

30.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 35 - 90 8.1e-11 Wall-associated receptor kinase galacturonan-binding
EGF_CA PF07645 295 - 329 6.8e-07 Calcium-binding EGF domain
Pkinase PF00069 421 - 685 4.8e-48 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 424 - 688 1.2e-45 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21210 AT1G21230 AT1G21240 AT1G21240 AT1G21245 AT1G21250 AT1G21270 AT1G22720 AT3G25490
fragaria_vesca FvH4_2g35051 FvH4_2g35051 FvH4_2g35060 FvH4_3g10281 FvH4_3g10285 FvH4_3g10286 FvH4_3g10311 FvH4_3g10311 FvH4_3g10320 FvH4_3g10332 FvH4_3g10340 FvH4_3g10350 FvH4_3g10360 FvH4_3g10390 FvH4_3g10412 FvH4_3g10413 FvH4_3g10461 FvH4_3g10462 FvH4_3g10470 FvH4_3g10510 FvH4_3g10520 FvH4_3g10530 FvH4_3g10550 FvH4_3g10560 FvH4_3g10611 FvH4_3g11730 FvH4_6g53140 FvH4_6g53142 FvH4_7g31880 FvH4_7g31910 FvH4_7g31920
malus_domestica MD05G1269900.v1.1 MD05G1270000.v1.1 MD05G1270200.v1.1 MD05G1270400.v1.1 MD05G1270500.v1.1 MD05G1270600.v1.1 MD05G1270800.v1.1 MD05G1270900.v1.1 MD07G1183800.v1.1 MD08G1107600.v1.1 MD10G1248500.v1.1 MD10G1249200.v1.1 MD10G1249500.v1.1 MD10G1250000.v1.1 MD10G1250500.v1.1 MD10G1250900.v1.1 MD10G1251200.v1.1 MD10G1251400.v1.1 MD13G1004600.v1.1 MD15G1088400.v1.1
prunus_persica Prupe.1G442400_v2.0.a1 Prupe.1G442500_v2.0.a1 Prupe.2G223400_v2.0.a1 Prupe.4G091900_v2.0.a1 Prupe.4G093200_v2.0.a1 Prupe.4G093300_v2.0.a1 Prupe.4G093400_v2.0.a1 Prupe.4G093500_v2.0.a1 Prupe.4G094100_v2.0.a1 Prupe.4G094200_v2.0.a1 Prupe.4G094300_v2.0.a1 Prupe.6G358300_v2.0.a1
pyrus_communis pycom08g08990 pycom10g20660 pycom10g20670 pycom10g20680 pycom10g20710 pycom10g20740 pycom13g00400 pycom15g08290
rosa_chinensis RchiOBHm_Chr1g0326861 RchiOBHm_Chr1g0326871 RchiOBHm_Chr1g0331391 RchiOBHm_Chr1g0336911 RchiOBHm_Chr1g0364001 RchiOBHm_Chr2g0174541 RchiOBHm_Chr2g0175041 RchiOBHm_Chr2g0175101 RchiOBHm_Chr2g0175121 RchiOBHm_Chr3g0486331 RchiOBHm_Chr5g0015581 RchiOBHm_Chr5g0016551 RchiOBHm_Chr5g0016561 RchiOBHm_Chr5g0016581 RchiOBHm_Chr5g0016601 RchiOBHm_Chr5g0016611 RchiOBHm_Chr5g0016641 RchiOBHm_Chr5g0016661 RchiOBHm_Chr5g0016691 RchiOBHm_Chr5g0016701 RchiOBHm_Chr5g0016731 RchiOBHm_Chr5g0016741 RchiOBHm_Chr5g0016811 RchiOBHm_Chr5g0016841 RchiOBHm_Chr5g0016871 RchiOBHm_Chr5g0016881 RchiOBHm_Chr5g0016931 RchiOBHm_Chr5g0017021 RchiOBHm_Chr5g0017041 RchiOBHm_Chr5g0017071 RchiOBHm_Chr5g0017091 RchiOBHm_Chr5g0017121 RchiOBHm_Chr5g0017141 RchiOBHm_Chr5g0017291 RchiOBHm_Chr5g0017321 RchiOBHm_Chr5g0017401 RchiOBHm_Chr5g0019001 RchiOBHm_Chr5g0019071 RchiOBHm_Chr5g0028941 RchiOBHm_Chr5g0052141 RchiOBHm_Chr5g0054721 RchiOBHm_Chr5g0054801 RchiOBHm_Chr5g0054931 RchiOBHm_Chr5g0054941 RchiOBHm_Chr6g0259291 RchiOBHm_Chr6g0292991 RchiOBHm_Chr6g0306911
rosa_laevigata RLG00000009465 RLG00000009468 RLG00000010755 RLG00000011976 RLG00000014541 RLG00000014542 RLG00000015169 RLG00000022308 RLG00000027529 RLG00000029324 RLG00000030244 RLG00000030245 RLG00000032210 RLG00000032211 RLG00000032257 RLG00000032259 RLG00000032260 RLG00000032261 RLG00000032262 RLG00000032268 RLG00000032269 RLG00000032272 RLG00000032282 RLG00000032283 RLG00000032308 RLG00000032413 RLG00000033129 RLG00000034945
rosa_multiflora Rmu_co8241929.1_g000001 Rmu_co8269227.1_g000001 Rmu_co8461259.1_g000001 Rmu_co8495385.1_g000001 Rmu_sc0000028.1_g000003 Rmu_sc0000083.1_g000009 Rmu_sc0000083.1_g000010 Rmu_sc0000083.1_g000031 Rmu_sc0000083.1_g000043 Rmu_sc0000083.1_g000046 Rmu_sc0000083.1_g000047 Rmu_sc0000563.1_g000024 Rmu_sc0000842.1_g000012 Rmu_sc0001235.1_g000001 Rmu_sc0001235.1_g000005 Rmu_sc0001235.1_g000009 Rmu_sc0001235.1_g000015 Rmu_sc0001235.1_g000016 Rmu_sc0001235.1_g000017 Rmu_sc0001473.1_g000036 Rmu_sc0001607.1_g000016 Rmu_sc0001788.1_g000006 Rmu_sc0002018.1_g000008 Rmu_sc0002028.1_g000001 Rmu_sc0002680.1_g000011 Rmu_sc0002798.1_g000023 Rmu_sc0002886.1_g000023 Rmu_sc0002989.1_g000012 Rmu_sc0003064.1_g000009 Rmu_sc0003382.1_g000006 Rmu_sc0003630.1_g000002 Rmu_sc0003630.1_g000047 Rmu_sc0003630.1_g000066 Rmu_sc0003630.1_g000067 Rmu_sc0003649.1_g000010 Rmu_sc0003688.1_g000005 Rmu_sc0003920.1_g000007 Rmu_sc0004765.1_g000007 Rmu_sc0004765.1_g000008 Rmu_sc0004920.1_g000011 Rmu_sc0005223.1_g000008 Rmu_sc0005223.1_g000010 Rmu_sc0005223.1_g000015 Rmu_sc0005223.1_g000016 Rmu_sc0005319.1_g000014 Rmu_sc0005319.1_g000015 Rmu_sc0005319.1_g000018 Rmu_sc0005319.1_g000019 Rmu_sc0005319.1_g000025 Rmu_sc0005418.1_g000007 Rmu_sc0005418.1_g000012 Rmu_sc0006106.1_g000011 Rmu_sc0006914.1_g000012 Rmu_sc0007023.1_g000001 Rmu_sc0007662.1_g000001 Rmu_sc0007920.1_g000002 Rmu_sc0007920.1_g000013 Rmu_sc0008053.1_g000016 Rmu_sc0008053.1_g000017 Rmu_sc0008053.1_g000018 Rmu_sc0008053.1_g000023 Rmu_sc0008053.1_g000050 Rmu_sc0009042.1_g000007 Rmu_sc0009042.1_g000008 Rmu_sc0010642.1_g000008 Rmu_sc0011109.1_g000002 Rmu_sc0011314.1_g000006 Rmu_sc0011974.1_g000006 Rmu_sc0012179.1_g000009 Rmu_sc0015169.1_g000001 Rmu_sc0016368.1_g000002 Rmu_sc0022659.1_g000001 Rmu_sc0027371.1_g000001 Rmu_sc0028213.1_g000001 Rmu_sc0034526.1_g000001 Rmu_sc0039366.1_g000002 Rmu_ssc0000050.1_g000084 Rmu_ssc0000050.1_g000088 Rmu_ssc0000167.1_g000017
rosa_roxburghii Rroxscaffold_1G00011340 Rroxscaffold_1G00025710 Rroxscaffold_1G00025870 Rroxscaffold_1G00026070 Rroxscaffold_1G00026170 Rroxscaffold_1G00026200 Rroxscaffold_1G00026270 Rroxscaffold_1G00050950 Rroxscaffold_1G00058900 Rroxscaffold_1G00060040 Rroxscaffold_1G00060060 Rroxscaffold_1G00060070 Rroxscaffold_1G00060080 Rroxscaffold_1G00060110 Rroxscaffold_1G00060120 Rroxscaffold_1G00060140 Rroxscaffold_1G00060170 Rroxscaffold_1G00060210 Rroxscaffold_1G00060220 Rroxscaffold_1G00060240 Rroxscaffold_1G00060250 Rroxscaffold_1G00060260 Rroxscaffold_1G00060300 Rroxscaffold_1G00060320 Rroxscaffold_1G00060330 Rroxscaffold_1G00060900 Rroxscaffold_1G00060920 Rroxscaffold_1G00073980 Rroxscaffold_2G00077560 Rroxscaffold_2G00077580 Rroxscaffold_2G00146990 Rroxscaffold_3G00236200 Rroxscaffold_3G00236210 Rroxscaffold_3G00236220 Rroxscaffold_4G00292760 Rroxscaffold_4G00315200 Rroxscaffold_4G00315240 Rroxscaffold_4G00326240 Rroxscaffold_4G00328980 Rroxscaffold_5G00341890 Rroxscaffold_7G00161350 Rroxscaffold_7G00174490 Rroxscaffold_7G00206550
rosa_rugosa Rorug01G0093000 Rorug01G0093100 Rorug01G0128800 Rorug01G0312000 Rorug01G0312000 Rorug02G0581100 Rorug03G0224600 Rorug05G0028500 Rorug05G0032700 Rorug05G0032800 Rorug05G0032900 Rorug05G0032900 Rorug05G0033000 Rorug05G0033100 Rorug05G0033300 Rorug05G0033400 Rorug05G0033900 Rorug05G0034900 Rorug05G0035000 Rorug05G0046900 Rorug05G0047500 Rorug05G0047500 Rorug05G0111600 Rorug05G0287900 Rorug05G0288700 Rorug05G0586900 Rorug06G0231700 Rorug06G0354300 Rorug06G0354400
rosa_samantha Rh1DG122200 Rh2DG686800 Rh5BG136600 Rh6AG344500 Rh6BG351900 Rh6CG359000 Rh6CG359100 Rh6CG387600 Rh6CG480500 Rh6DG344600 Rh6DG467400
rosa_wichuraiana Rw0G010250 Rw0G014820 Rw0G021400 Rw0G023100 Rw1G009450 Rw1G012100 Rw1G012180 Rw1G028360 Rw2G054260 Rw3G024370 Rw3G024420 Rw3G024470 Rw5G010850 Rw5G011010 Rw5G011020 Rw5G011050 Rw5G011080 Rw5G011120 Rw5G012300 Rw5G018470 Rw5G033830 Rw6G008930 Rw6G030070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 207
Acc65I GGTACC 2 cut(s) 975, 1702
AccB1I GGYRCC 4 cut(s) 975, 1199, 1688, 1702
AccB7I CCANNNNNTGG 1 cut(s) 82
AccII CGCG 1 cut(s) 776
AciI CCGC 5 cut(s) 173, 352, 967, 1908, 1924
AclI AACGTT 1 cut(s) 2165
AclWI GGATC 3 cut(s) 896, 909, 1719
AcsI RAATTY 3 cut(s) 337, 362, 1314
AcuI CTGAAG 2 cut(s) 1129, 1151
AfiI CCNNNNNNNGG 3 cut(s) 82, 172, 522
AflII CTTAAG 1 cut(s) 1979
AflIII ACRYGT 3 cut(s) 476, 794, 1079
AgsI TTSAA 8 cut(s) 277, 360, 613, 655, 752, 1037, 1775, 2014
AhlI ACTAGT 2 cut(s) 64, 1474
AjnI CCWGG 3 cut(s) 435, 780, 2096
AjuI GAANNNNNNNTTGG 2 cut(s) 2127, 2159
Alw21I GWGCWC 1 cut(s) 925
Alw26I GTCTC 3 cut(s) 728, 1262, 1451
Alw44I GTGCAC 1 cut(s) 921
AlwI GGATC 3 cut(s) 896, 909, 1719
AlwNI CAGNNNCTG 1 cut(s) 918
Ama87I CYCGRG 1 cut(s) 1748
AoxI GGCC 2 cut(s) 1060, 1248
ApaLI GTGCAC 1 cut(s) 921
ApeKI GCWGC 7 cut(s) 31, 465, 621, 771, 1571, 1601, 1975
ApoI RAATTY 3 cut(s) 337, 362, 1314
AseI ATTAAT 2 cut(s) 1163, 1389
Asp700I GAANNNNTTC 1 cut(s) 1770
Asp718I GGTACC 2 cut(s) 975, 1702
AspLEI GCGC 2 cut(s) 776, 1855
AsuHPI GGTGA 3 cut(s) 1444, 1483, 2159
AvaI CYCGRG 1 cut(s) 1748
BaeGI GKGCMC 1 cut(s) 925
BamHI GGATCC 1 cut(s) 901
BanI GGYRCC 4 cut(s) 975, 1199, 1688, 1702
BarI GAAGNNNNNNTAC 2 cut(s) 684, 716
Bbv12I GWGCWC 1 cut(s) 925
BbvI GCAGC 7 cut(s) 18, 452, 633, 783, 1558, 1588, 1987
BccI CCATC 7 cut(s) 260, 836, 1107, 1319, 2038, 2074, 2237
BcgI CGANNNNNNTGC 2 cut(s) 816, 850
BciT130I CCWGG 3 cut(s) 437, 782, 2098
BciVI GTATCC 1 cut(s) 815
BclI TGATCA 1 cut(s) 1363
BcoDI GTCTC 3 cut(s) 728, 1262, 1451
BcuI ACTAGT 2 cut(s) 64, 1474
BfaI CTAG 4 cut(s) 65, 1272, 1475, 1889
BfmI CTRYAG 3 cut(s) 1105, 1230, 1807
BfoI RGCGCY 1 cut(s) 1856
BfrI CTTAAG 1 cut(s) 1979
BfuI GTATCC 1 cut(s) 815
BisI GCNGC 8 cut(s) 32, 466, 622, 772, 967, 1572, 1602, 1976
BlsI GCNGC 8 cut(s) 33, 467, 623, 773, 968, 1573, 1603, 1977
BmcAI AGTACT 2 cut(s) 699, 1398
Bme1390I CCNGG 3 cut(s) 437, 782, 2098
BmeT110I CYCGRG 1 cut(s) 1748
BmiI GGNNCC 7 cut(s) 857, 903, 977, 1201, 1690, 1704, 2219
BmrFI CCNGG 3 cut(s) 437, 782, 2098
BmsI GCATC 5 cut(s) 160, 353, 818, 1544, 2098
BoxI GACNNNNGTC 1 cut(s) 1463
BplI GAGNNNNNCTC 2 cut(s) 657, 689
BpmI CTGGAG 3 cut(s) 646, 1596, 1678
BpuEI CTTGAG 3 cut(s) 282, 1262, 2084
BsaBI GATNNNNATC 2 cut(s) 846, 1884
BsaJI CCNNGG 6 cut(s) 427, 436, 781, 979, 2096, 2097
BsaWI WCCGGW 1 cut(s) 515
Bsc4I CCNNNNNNNGG 3 cut(s) 82, 172, 522
Bse1I ACTGG 4 cut(s) 1404, 1494, 2163, 2234
Bse3DI GCAATG 5 cut(s) 127, 393, 991, 1899, 2091
Bse8I GATNNNNATC 2 cut(s) 846, 1884
BseBI CCWGG 3 cut(s) 437, 782, 2098
BseDI CCNNGG 6 cut(s) 427, 436, 781, 979, 2096, 2097
BseGI GGATG 9 cut(s) 18, 71, 410, 694, 847, 1515, 1535, 2085, 2092
BseJI GATNNNNATC 2 cut(s) 846, 1884
BseLI CCNNNNNNNGG 3 cut(s) 82, 172, 522
BseMI GCAATG 5 cut(s) 127, 393, 991, 1899, 2091
BseMII CTCAG 4 cut(s) 218, 925, 1611, 1710
BseNI ACTGG 4 cut(s) 1404, 1494, 2163, 2234
BseRI GAGGAG 1 cut(s) 1889
BseSI GKGCMC 1 cut(s) 925
BseXI GCAGC 7 cut(s) 18, 452, 633, 783, 1558, 1588, 1987
Bsh1236I CGCG 1 cut(s) 776
BshFI GGCC 2 cut(s) 1062, 1250
BshNI GGYRCC 4 cut(s) 975, 1199, 1688, 1702
BsiHKAI GWGCWC 1 cut(s) 925
BsiHKCI CYCGRG 1 cut(s) 1748
BsiSI CCGG 1 cut(s) 516
BslFI GGGAC 3 cut(s) 1451, 1595, 2082
BslI CCNNNNNNNGG 3 cut(s) 82, 172, 522
BsmAI GTCTC 3 cut(s) 728, 1262, 1451
BsmFI GGGAC 3 cut(s) 1451, 1595, 2082
BsmI GAATGC 1 cut(s) 129
BsnI GGCC 2 cut(s) 1062, 1250
BsoBI CYCGRG 1 cut(s) 1748
Bsp1286I GDGCHC 1 cut(s) 925
Bsp1407I TGTACA 1 cut(s) 1305
Bsp143I GATC 3 cut(s) 901, 1363, 1711
Bsp19I CCATGG 1 cut(s) 979
BspACI CCGC 5 cut(s) 173, 352, 967, 1908, 1924
BspANI GGCC 2 cut(s) 1062, 1250
BspCNI CTCAG 4 cut(s) 219, 924, 1610, 1711
BspFNI CGCG 1 cut(s) 776
BspHI TCATGA 2 cut(s) 447, 1122
BspLI GGNNCC 7 cut(s) 857, 903, 977, 1201, 1690, 1704, 2219
BspMAI CTGCAG 1 cut(s) 1234
BspPI GGATC 3 cut(s) 896, 909, 1719
BspT107I GGYRCC 4 cut(s) 975, 1199, 1688, 1702
BspTI CTTAAG 1 cut(s) 1979
BsrDI GCAATG 5 cut(s) 127, 393, 991, 1899, 2091
BsrGI TGTACA 1 cut(s) 1305
BsrI ACTGG 4 cut(s) 1404, 1494, 2163, 2234
BssECI CCNNGG 6 cut(s) 427, 436, 781, 979, 2096, 2097
BssMI GATC 3 cut(s) 901, 1363, 1711
BssT1I CCWWGG 1 cut(s) 979
Bst2UI CCWGG 3 cut(s) 437, 782, 2098
Bst4CI ACNGT 8 cut(s) 82, 581, 697, 920, 959, 1045, 1298, 1303
Bst6I CTCTTC 1 cut(s) 1324
BstAFI CTTAAG 1 cut(s) 1979
BstAPI GCANNNNNTGC 2 cut(s) 40, 777
BstAUI TGTACA 1 cut(s) 1305
BstC8I GCNNGC 7 cut(s) 743, 769, 778, 1745, 2056, 2060, 2141
BstDEI CTNAG 6 cut(s) 227, 911, 1597, 1719, 1756, 2072
BstDSI CCRYGG 1 cut(s) 979
BstF5I GGATG 9 cut(s) 18, 71, 410, 694, 847, 1515, 1535, 2085, 2092
BstFNI CGCG 1 cut(s) 776
BstH2I RGCGCY 1 cut(s) 1856
BstHHI GCGC 2 cut(s) 776, 1855
BstKTI GATC 3 cut(s) 904, 1366, 1714
BstMAI GTCTC 3 cut(s) 728, 1262, 1451
BstMBI GATC 3 cut(s) 901, 1363, 1711
BstMWI GCNNNNNNNGC 7 cut(s) 40, 126, 438, 773, 777, 1022, 1290
BstNI CCWGG 3 cut(s) 437, 782, 2098
BstNSI RCATGY 2 cut(s) 480, 798
BstPAI GACNNNNGTC 1 cut(s) 1463
BstSCI CCNGG 3 cut(s) 435, 780, 2096
BstSFI CTRYAG 3 cut(s) 1105, 1230, 1807
BstSLI GKGCMC 1 cut(s) 925
BstUI CGCG 1 cut(s) 776
BstV1I GCAGC 7 cut(s) 18, 452, 633, 783, 1558, 1588, 1987
BstX2I RGATCY 1 cut(s) 901
BstXI CCANNNNNNTGG 1 cut(s) 986
BstYI RGATCY 1 cut(s) 901
BsuI GTATCC 1 cut(s) 815
BsuRI GGCC 2 cut(s) 1062, 1250
BtgI CCRYGG 1 cut(s) 979
BtsCI GGATG 9 cut(s) 18, 71, 410, 694, 847, 1515, 1535, 2085, 2092
BtsIMutI CAGTG 6 cut(s) 87, 1041, 1308, 1501, 1910, 2156
Cac8I GCNNGC 7 cut(s) 743, 769, 778, 1745, 2056, 2060, 2141
CaiI CAGNNNCTG 1 cut(s) 918
CciI TCATGA 2 cut(s) 447, 1122
CfoI GCGC 2 cut(s) 776, 1855
DdeI CTNAG 6 cut(s) 227, 911, 1597, 1719, 1756, 2072
DpnI GATC 3 cut(s) 903, 1365, 1713
DpnII GATC 3 cut(s) 901, 1363, 1711
DraI TTTAAA 1 cut(s) 684
Eam1104I CTCTTC 1 cut(s) 1324
EarI CTCTTC 1 cut(s) 1324
Eco130I CCWWGG 1 cut(s) 979
Eco57I CTGAAG 2 cut(s) 1129, 1151
Eco88I CYCGRG 1 cut(s) 1748
EcoRII CCWGG 3 cut(s) 435, 780, 2096
EcoT14I CCWWGG 1 cut(s) 979
EcoT22I ATGCAT 1 cut(s) 2091
ErhI CCWWGG 1 cut(s) 979
FalI AAGNNNNNCTT 2 cut(s) 641, 673
FaqI GGGAC 3 cut(s) 1451, 1595, 2082
FauI CCCGC 1 cut(s) 359
FbaI TGATCA 1 cut(s) 1363
Fnu4HI GCNGC 8 cut(s) 32, 466, 622, 772, 967, 1572, 1602, 1976
FokI GGATG 9 cut(s) 25, 58, 417, 701, 854, 1502, 1522, 2079, 2092
Fsp4HI GCNGC 8 cut(s) 32, 466, 622, 772, 967, 1572, 1602, 1976
FspBI CTAG 4 cut(s) 65, 1272, 1475, 1889
GlaI GCGC 2 cut(s) 775, 1854
GluI GCNGC 8 cut(s) 32, 466, 622, 772, 967, 1572, 1602, 1976
GsuI CTGGAG 3 cut(s) 646, 1596, 1678
HaeII RGCGCY 1 cut(s) 1856
HaeIII GGCC 2 cut(s) 1062, 1250
HapII CCGG 1 cut(s) 516
HhaI GCGC 2 cut(s) 776, 1855
Hin6I GCGC 2 cut(s) 774, 1853
HinP1I GCGC 2 cut(s) 774, 1853
HindIII AAGCTT 2 cut(s) 1436, 2137
HinfI GANTC 7 cut(s) 311, 423, 506, 589, 1140, 1275, 2114
HpaII CCGG 1 cut(s) 516
HphI GGTGA 3 cut(s) 1444, 1483, 2159
Hpy166II GTNNAC 4 cut(s) 456, 923, 1306, 1638
Hpy188I TCNGA 9 cut(s) 146, 216, 228, 428, 1148, 1186, 1324, 1681, 1956
Hpy8I GTNNAC 4 cut(s) 456, 923, 1306, 1638
HpyAV CCTTC 6 cut(s) 746, 998, 1126, 1240, 1843, 2070
HpyCH4III ACNGT 8 cut(s) 82, 581, 697, 920, 959, 1045, 1298, 1303
HpyCH4IV ACGT 2 cut(s) 1079, 2165
HpyF10VI GCNNNNNNNGC 7 cut(s) 40, 126, 438, 773, 777, 1022, 1290
HpyF3I CTNAG 6 cut(s) 227, 911, 1597, 1719, 1756, 2072
HpySE526I ACGT 2 cut(s) 1079, 2165
HspAI GCGC 2 cut(s) 774, 1853
KpnI GGTACC 2 cut(s) 979, 1706
Ksp22I TGATCA 1 cut(s) 1363
Kzo9I GATC 3 cut(s) 901, 1363, 1711
LmnI GCTCC 1 cut(s) 1831
Lsp1109I GCAGC 7 cut(s) 18, 452, 633, 783, 1558, 1588, 1987
LweI GCATC 5 cut(s) 160, 353, 818, 1544, 2098
MaeI CTAG 4 cut(s) 65, 1272, 1475, 1889
MaeII ACGT 2 cut(s) 1079, 2165
MalI GATC 3 cut(s) 903, 1365, 1713
MboI GATC 3 cut(s) 901, 1363, 1711
MboII GAAGA 8 cut(s) 28, 653, 1013, 1122, 1135, 1247, 1341, 2039
MfeI CAATTG 4 cut(s) 35, 540, 723, 1784
MflI RGATCY 1 cut(s) 901
MhlI GDGCHC 1 cut(s) 925
MluCI AATT 9 cut(s) 35, 337, 362, 540, 723, 1050, 1314, 1382, 1784
MmeI TCCRAC 4 cut(s) 382, 399, 1027, 1563
Mph1103I ATGCAT 1 cut(s) 2091
MroXI GAANNNNTTC 1 cut(s) 1770
MseI TTAA 6 cut(s) 683, 1163, 1389, 1562, 1980, 2103
MslI CAYNNNNRTG 7 cut(s) 564, 597, 928, 984, 1425, 1626, 2178
MspA1I CMGCKG 1 cut(s) 1601
MspCI CTTAAG 1 cut(s) 1979
MspI CCGG 1 cut(s) 516
MspR9I CCNGG 3 cut(s) 437, 782, 2098
MunI CAATTG 4 cut(s) 35, 540, 723, 1784
Mva1269I GAATGC 1 cut(s) 129
MvaI CCWGG 3 cut(s) 437, 782, 2098
MvnI CGCG 1 cut(s) 776
MwoI GCNNNNNNNGC 7 cut(s) 40, 126, 438, 773, 777, 1022, 1290
NcoI CCATGG 1 cut(s) 979
NdeII GATC 3 cut(s) 901, 1363, 1711
NlaIV GGNNCC 7 cut(s) 857, 903, 977, 1201, 1690, 1704, 2219
NmeAIII GCCGAG 1 cut(s) 1080
NmuCI GTSAC 4 cut(s) 247, 407, 479, 1489
NsiI ATGCAT 1 cut(s) 2091
NspI RCATGY 2 cut(s) 480, 798
PagI TCATGA 2 cut(s) 447, 1122
PasI CCCWGGG 1 cut(s) 2097
PciI ACATGT 2 cut(s) 476, 794
PcsI WCGNNNNNNNCGW 1 cut(s) 1076
PctI GAATGC 1 cut(s) 129
PdmI GAANNNNTTC 1 cut(s) 1770
PfeI GAWTC 7 cut(s) 311, 423, 506, 589, 1140, 1275, 2114
PflMI CCANNNNNTGG 1 cut(s) 82
PkrI GCNGC 8 cut(s) 33, 467, 623, 773, 968, 1573, 1603, 1977
PscI ACATGT 2 cut(s) 476, 794
PshAI GACNNNNGTC 1 cut(s) 1463
PshBI ATTAAT 2 cut(s) 1163, 1389
PsiI TTATAA 1 cut(s) 207
Psp1406I AACGTT 1 cut(s) 2165
Psp6I CCWGG 3 cut(s) 435, 780, 2096
PspGI CCWGG 3 cut(s) 435, 780, 2096
PspN4I GGNNCC 7 cut(s) 857, 903, 977, 1201, 1690, 1704, 2219
PstI CTGCAG 1 cut(s) 1234
PstNI CAGNNNCTG 1 cut(s) 918
PsuI RGATCY 1 cut(s) 901
PvuII CAGCTG 1 cut(s) 1601
RseI CAYNNNNRTG 7 cut(s) 564, 597, 928, 984, 1425, 1626, 2178
SaqAI TTAA 6 cut(s) 683, 1163, 1389, 1562, 1980, 2103
SatI GCNGC 8 cut(s) 32, 466, 622, 772, 967, 1572, 1602, 1976
Sau3AI GATC 3 cut(s) 901, 1363, 1711
ScaI AGTACT 2 cut(s) 699, 1398
ScrFI CCNGG 3 cut(s) 437, 782, 2098
SduI GDGCHC 1 cut(s) 925
SfaNI GCATC 5 cut(s) 160, 353, 818, 1544, 2098
SfcI CTRYAG 3 cut(s) 1105, 1230, 1807
SmiMI CAYNNNNRTG 7 cut(s) 564, 597, 928, 984, 1425, 1626, 2178
SmlI CTYRAG 4 cut(s) 297, 1241, 1979, 2063
SmoI CTYRAG 4 cut(s) 297, 1241, 1979, 2063
SpeI ACTAGT 2 cut(s) 64, 1474
Sse9I AATT 9 cut(s) 35, 337, 362, 540, 723, 1050, 1314, 1382, 1784
SsiI CCGC 5 cut(s) 173, 352, 967, 1908, 1924
SspMI CTAG 4 cut(s) 65, 1272, 1475, 1889
StyD4I CCNGG 3 cut(s) 435, 780, 2096
StyI CCWWGG 1 cut(s) 979
TaaI ACNGT 8 cut(s) 82, 581, 697, 920, 959, 1045, 1298, 1303
TaiI ACGT 2 cut(s) 1082, 2168
TaqI TCGA 5 cut(s) 347, 807, 1455, 1727, 1863
TasI AATT 9 cut(s) 35, 337, 362, 540, 723, 1050, 1314, 1382, 1784
TatI WGTACW 4 cut(s) 453, 697, 1305, 1396
TauI GCSGC 1 cut(s) 969
TfiI GAWTC 7 cut(s) 311, 423, 506, 589, 1140, 1275, 2114
Tru1I TTAA 6 cut(s) 683, 1163, 1389, 1562, 1980, 2103
Tru9I TTAA 6 cut(s) 683, 1163, 1389, 1562, 1980, 2103
TscAI CASTG 6 cut(s) 87, 1048, 1308, 1501, 1917, 2163
TseFI GTSAC 4 cut(s) 247, 407, 479, 1489
TseI GCWGC 7 cut(s) 31, 465, 621, 771, 1571, 1601, 1975
Tsp45I GTSAC 4 cut(s) 247, 407, 479, 1489
TspGWI ACGGA 1 cut(s) 314
TspRI CASTG 6 cut(s) 87, 1048, 1308, 1501, 1917, 2163
Van91I CCANNNNNTGG 1 cut(s) 82
Vha464I CTTAAG 1 cut(s) 1979
VneI GTGCAC 1 cut(s) 921
VspI ATTAAT 2 cut(s) 1163, 1389
XapI RAATTY 3 cut(s) 337, 362, 1314
XceI RCATGY 2 cut(s) 480, 798
XcmI CCANNNNNNNNNTGG 1 cut(s) 113
XmnI GAANNNNTTC 1 cut(s) 1770
XspI CTAG 4 cut(s) 65, 1272, 1475, 1889
ZrmI AGTACT 2 cut(s) 699, 1398
Zsp2I ATGCAT 1 cut(s) 2091
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.