Rw1G009450

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000066
Physical Location & Seq
Reverse (-)
20820914 .. 20823840
2927 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G009450.1

Sequence Viewer

Length: 2346 bp
ATGTCTACGACCATAAAGAAAACATTTTTGCTGCGATTGGTGCTGGTTGCAATAGAGGTGCTACTAGTATCATCCCAAACGGTGGATATTACAAGAGAGGGTTGCCAAGACAAATGTGGCAATGTTAGCATTCCCTATCCGTTTGGAACAAGAGAGGGTTGTTACTATGATAAGGATTTCCTCATTACTTGTAATAGCGCAAGTTATAACCCTCCGAAGCCATTTCTTCGAGTGAGTGACGTTGAGGTCACAAACGTATCCATTGATGGGAAACTCTATATTTCTCAAGACATAGCCAGGGCTTGTTTTACCGAATCAGGTGCGCCCTATTATGGCAACTACAACCCTTATCTCTCTTTGAAAAAGTTCTACATCTCAGACACCGACAACAAATTCACAGGTGTTGGTTGTGACACCTATGCCGAAATTAAGGGCTACCAGGGTATGAGCCTCATGTACACAGGTGGCTGCTTTTCAAAATGTCAAAGCACGGCTTCTATTTCTAATTATTCATGCTCTGGCATAGGTTGTTGCCAAACACCTATTGCTAAAGGAATGACTTACTTTACCGTAAGCGTAAGCAGCTACAATAATCAAACGGAAGTGTGGAATTTCAATCCCTGCAGCTATGCTTTTCTTGTCCAAGAGGGTAAGTTCAACTTCACCTCCGACATGTTGAGCGATTTAGGAAATGTCTCTCAGTTTCCAGTGGTTCTCGATTGGTCGATTGGAAATGAGCCATGCAGCCAGATCAAGAACAATGCATGTCAGGGGAACAGCAAGTGTTTGGATGTCAAGAATGGGCTCGGGTATCGCTGCAAGTGCAACGAAGGTTACGAAGGGAATCCATACCTAAATGGATGCACTGACATTAATGAATGCGAGGATCCGGTGATTTCCAACAAGTGCAAACAAAGATGCATAAATACAGAGGGGAACTATACGTGTGCTTGCCACAAGGGGTACCATAACAATGGCACAACAGACTCGGTAGATTGCATCGCTGACCACCAAACAATATTTGTTCAAATTATACTTGGTAAGTGCAATAGCAATACTCTGCTCCATTTGGTTACGAAAAATACCAGAACTACTAATGCTTGGATGTGTGTTGGAAATGCAGGGCTCGGTGTTGGCCTCATATCCTTATTAATAGGCATCTCTTGGTTGTACTTGGTCTACAAAAGATGGAAGCTCATGAAGCTCAAGGAGAAGTTCTTCATCCAAAATGGAGGATTGATGTTAAAGCAACAGCTTTCGGAACGACAAGGTGGTGCCTCTAACCAAACGGCCAAAATCTTCACCGAATACCAACTTAAGAAGGCTACCAACCATTTCAGTGAAGCCAGAATCGTTGGCAAAGGAGGTTTCGGTACAGTCTACAAAGGGATTATAGCAGATGAAAGAGCTAAGGAAAAAGTGGTTGCAATCAAGAAATCCAAACTTGTGGATCGAAGCCAAATAGAGCAATTCATTAATGAAGTAGTGGTGCTTTCCCAAATTAATCACAGGAATGTGGTGAAGCTTTTGGGTTGCTGTTTCGAGACAGAAGTCCCACTACTGGTTTATGAGTTTGTCACCAATGGTACGCTATTCGATTACATCCACAACAAAAGCAAGGAATCCAACTTTGCTTGGGAAATTCGTTTAAGGATAGCTGCAGAAACTGCTGGAGTGCTATCACACTTGCACTCTGAAGCTTCTATTCCCATCATTCACAGAGATGTCAAGTCCACAAACATACTCTTGGATGATAATCTTACAGCAAAAGTGTCTGATTTCGGTGCCTCCAGATTGGTTCCGTCAGATCAAGCTCAGCTCTCTACAGTGGTGCAAGGAACGGTAGGGTATTTGGACCCTGAATACTTACAGACGAGCCAGTTGACTGACAAGAGTGATGTCTATAGCTTTGGGGTTGTTCTTGTGGAACTACTAACAGGAAAGAAGGCGCTTTCGGTGGACAAGCCGGAAAAGGAGAGAAATCTAGCCATGTATTTTCTTTCTGCCCTAAGAGAGGACCGATTGGTAGAGGTGATTGATGGTAGTGTCATTAATGGAGCAAACGTTGAGCAGCTTAAGGAAGTTTCAAACCTTGCAAAAAGGTGTTTGAAAGTAGAAGGAAAAGAAAGACCAACCATGAAGGAAGTGGCAATGGAGCTTGAGGGACTGAGAAGGATGGTAATGCATCCATGGGTTAGTAATGATGAATCCAATGCAGAGGAGAATGAACACTTGCTTCATGAAATAACACTGGAAACTTTTAGTCATGGAGGTGGTGGGGATACGAGTTCTGGGTATGATACCATGAGGAACCTGATCACATTACCAGTTGATGATGGGAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF_CA GUB_WAK_bind Pkinase WAK

Protein Analysis

781

Amino Acids

86.76

Weight (kDa)

6.22

Isoelectric Point (pI)

28.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 35 - 90 7.7e-10 Wall-associated receptor kinase galacturonan-binding
EGF_CA PF07645 290 - 327 3.5e-06 Calcium-binding EGF domain
Pkinase PF00069 447 - 716 1.2e-48 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 450 - 720 2.2e-49 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21210 AT1G21230 AT1G21240 AT1G21240 AT1G21245 AT1G21250 AT1G21270 AT1G22720 AT3G25490
fragaria_vesca FvH4_2g35051 FvH4_2g35051 FvH4_2g35060 FvH4_3g10281 FvH4_3g10285 FvH4_3g10286 FvH4_3g10311 FvH4_3g10311 FvH4_3g10320 FvH4_3g10332 FvH4_3g10340 FvH4_3g10350 FvH4_3g10360 FvH4_3g10390 FvH4_3g10412 FvH4_3g10413 FvH4_3g10461 FvH4_3g10462 FvH4_3g10470 FvH4_3g10510 FvH4_3g10520 FvH4_3g10530 FvH4_3g10550 FvH4_3g10560 FvH4_3g10611 FvH4_3g11730 FvH4_6g53140 FvH4_6g53142 FvH4_7g31880 FvH4_7g31910 FvH4_7g31920
malus_domestica MD05G1269900.v1.1 MD05G1270000.v1.1 MD05G1270200.v1.1 MD05G1270400.v1.1 MD05G1270500.v1.1 MD05G1270600.v1.1 MD05G1270800.v1.1 MD05G1270900.v1.1 MD07G1183800.v1.1 MD08G1107600.v1.1 MD10G1248500.v1.1 MD10G1249200.v1.1 MD10G1249500.v1.1 MD10G1250000.v1.1 MD10G1250500.v1.1 MD10G1250900.v1.1 MD10G1251200.v1.1 MD10G1251400.v1.1 MD13G1004600.v1.1 MD15G1088400.v1.1
prunus_persica Prupe.1G442400_v2.0.a1 Prupe.1G442500_v2.0.a1 Prupe.2G223400_v2.0.a1 Prupe.4G091900_v2.0.a1 Prupe.4G093200_v2.0.a1 Prupe.4G093300_v2.0.a1 Prupe.4G093400_v2.0.a1 Prupe.4G093500_v2.0.a1 Prupe.4G094100_v2.0.a1 Prupe.4G094200_v2.0.a1 Prupe.4G094300_v2.0.a1 Prupe.6G358300_v2.0.a1
pyrus_communis pycom08g08990 pycom10g20660 pycom10g20670 pycom10g20680 pycom10g20710 pycom10g20740 pycom13g00400 pycom15g08290
rosa_chinensis RchiOBHm_Chr1g0326861 RchiOBHm_Chr1g0326871 RchiOBHm_Chr1g0331391 RchiOBHm_Chr1g0336911 RchiOBHm_Chr1g0364001 RchiOBHm_Chr2g0174541 RchiOBHm_Chr2g0175041 RchiOBHm_Chr2g0175101 RchiOBHm_Chr2g0175121 RchiOBHm_Chr3g0486331 RchiOBHm_Chr5g0015581 RchiOBHm_Chr5g0016551 RchiOBHm_Chr5g0016561 RchiOBHm_Chr5g0016581 RchiOBHm_Chr5g0016601 RchiOBHm_Chr5g0016611 RchiOBHm_Chr5g0016641 RchiOBHm_Chr5g0016661 RchiOBHm_Chr5g0016691 RchiOBHm_Chr5g0016701 RchiOBHm_Chr5g0016731 RchiOBHm_Chr5g0016741 RchiOBHm_Chr5g0016811 RchiOBHm_Chr5g0016841 RchiOBHm_Chr5g0016871 RchiOBHm_Chr5g0016881 RchiOBHm_Chr5g0016931 RchiOBHm_Chr5g0017021 RchiOBHm_Chr5g0017041 RchiOBHm_Chr5g0017071 RchiOBHm_Chr5g0017091 RchiOBHm_Chr5g0017121 RchiOBHm_Chr5g0017141 RchiOBHm_Chr5g0017291 RchiOBHm_Chr5g0017321 RchiOBHm_Chr5g0017401 RchiOBHm_Chr5g0019001 RchiOBHm_Chr5g0019071 RchiOBHm_Chr5g0028941 RchiOBHm_Chr5g0052141 RchiOBHm_Chr5g0054721 RchiOBHm_Chr5g0054801 RchiOBHm_Chr5g0054931 RchiOBHm_Chr5g0054941 RchiOBHm_Chr6g0259291 RchiOBHm_Chr6g0292991 RchiOBHm_Chr6g0306911
rosa_laevigata RLG00000009465 RLG00000009468 RLG00000010755 RLG00000011976 RLG00000014541 RLG00000014542 RLG00000015169 RLG00000022308 RLG00000027529 RLG00000029324 RLG00000030244 RLG00000030245 RLG00000032210 RLG00000032211 RLG00000032257 RLG00000032259 RLG00000032260 RLG00000032261 RLG00000032262 RLG00000032268 RLG00000032269 RLG00000032272 RLG00000032282 RLG00000032283 RLG00000032308 RLG00000032413 RLG00000033129 RLG00000034945
rosa_multiflora Rmu_co8241929.1_g000001 Rmu_co8269227.1_g000001 Rmu_co8461259.1_g000001 Rmu_co8495385.1_g000001 Rmu_sc0000028.1_g000003 Rmu_sc0000083.1_g000009 Rmu_sc0000083.1_g000010 Rmu_sc0000083.1_g000031 Rmu_sc0000083.1_g000043 Rmu_sc0000083.1_g000046 Rmu_sc0000083.1_g000047 Rmu_sc0000563.1_g000024 Rmu_sc0000842.1_g000012 Rmu_sc0001235.1_g000001 Rmu_sc0001235.1_g000005 Rmu_sc0001235.1_g000009 Rmu_sc0001235.1_g000015 Rmu_sc0001235.1_g000016 Rmu_sc0001235.1_g000017 Rmu_sc0001473.1_g000036 Rmu_sc0001607.1_g000016 Rmu_sc0001788.1_g000006 Rmu_sc0002018.1_g000008 Rmu_sc0002028.1_g000001 Rmu_sc0002680.1_g000011 Rmu_sc0002798.1_g000023 Rmu_sc0002886.1_g000023 Rmu_sc0002989.1_g000012 Rmu_sc0003064.1_g000009 Rmu_sc0003382.1_g000006 Rmu_sc0003630.1_g000002 Rmu_sc0003630.1_g000047 Rmu_sc0003630.1_g000066 Rmu_sc0003630.1_g000067 Rmu_sc0003649.1_g000010 Rmu_sc0003688.1_g000005 Rmu_sc0003920.1_g000007 Rmu_sc0004765.1_g000007 Rmu_sc0004765.1_g000008 Rmu_sc0004920.1_g000011 Rmu_sc0005223.1_g000008 Rmu_sc0005223.1_g000010 Rmu_sc0005223.1_g000015 Rmu_sc0005223.1_g000016 Rmu_sc0005319.1_g000014 Rmu_sc0005319.1_g000015 Rmu_sc0005319.1_g000018 Rmu_sc0005319.1_g000019 Rmu_sc0005319.1_g000025 Rmu_sc0005418.1_g000007 Rmu_sc0005418.1_g000012 Rmu_sc0006106.1_g000011 Rmu_sc0006914.1_g000012 Rmu_sc0007023.1_g000001 Rmu_sc0007662.1_g000001 Rmu_sc0007920.1_g000002 Rmu_sc0007920.1_g000013 Rmu_sc0008053.1_g000016 Rmu_sc0008053.1_g000017 Rmu_sc0008053.1_g000018 Rmu_sc0008053.1_g000023 Rmu_sc0008053.1_g000050 Rmu_sc0009042.1_g000007 Rmu_sc0009042.1_g000008 Rmu_sc0010642.1_g000008 Rmu_sc0011109.1_g000002 Rmu_sc0011314.1_g000006 Rmu_sc0011974.1_g000006 Rmu_sc0012179.1_g000009 Rmu_sc0015169.1_g000001 Rmu_sc0016368.1_g000002 Rmu_sc0022659.1_g000001 Rmu_sc0027371.1_g000001 Rmu_sc0028213.1_g000001 Rmu_sc0034526.1_g000001 Rmu_sc0039366.1_g000002 Rmu_ssc0000050.1_g000084 Rmu_ssc0000050.1_g000088 Rmu_ssc0000167.1_g000017
rosa_roxburghii Rroxscaffold_1G00011340 Rroxscaffold_1G00025710 Rroxscaffold_1G00025870 Rroxscaffold_1G00026070 Rroxscaffold_1G00026170 Rroxscaffold_1G00026200 Rroxscaffold_1G00026270 Rroxscaffold_1G00050950 Rroxscaffold_1G00058900 Rroxscaffold_1G00060040 Rroxscaffold_1G00060060 Rroxscaffold_1G00060070 Rroxscaffold_1G00060080 Rroxscaffold_1G00060110 Rroxscaffold_1G00060120 Rroxscaffold_1G00060140 Rroxscaffold_1G00060170 Rroxscaffold_1G00060210 Rroxscaffold_1G00060220 Rroxscaffold_1G00060240 Rroxscaffold_1G00060250 Rroxscaffold_1G00060260 Rroxscaffold_1G00060300 Rroxscaffold_1G00060320 Rroxscaffold_1G00060330 Rroxscaffold_1G00060900 Rroxscaffold_1G00060920 Rroxscaffold_1G00073980 Rroxscaffold_2G00077560 Rroxscaffold_2G00077580 Rroxscaffold_2G00146990 Rroxscaffold_3G00236200 Rroxscaffold_3G00236210 Rroxscaffold_3G00236220 Rroxscaffold_4G00292760 Rroxscaffold_4G00315200 Rroxscaffold_4G00315240 Rroxscaffold_4G00326240 Rroxscaffold_4G00328980 Rroxscaffold_5G00341890 Rroxscaffold_7G00161350 Rroxscaffold_7G00174490 Rroxscaffold_7G00206550
rosa_rugosa Rorug01G0093000 Rorug01G0093100 Rorug01G0128800 Rorug01G0312000 Rorug01G0312000 Rorug02G0581100 Rorug03G0224600 Rorug05G0028500 Rorug05G0032700 Rorug05G0032800 Rorug05G0032900 Rorug05G0032900 Rorug05G0033000 Rorug05G0033100 Rorug05G0033300 Rorug05G0033400 Rorug05G0033900 Rorug05G0034900 Rorug05G0035000 Rorug05G0046900 Rorug05G0047500 Rorug05G0047500 Rorug05G0111600 Rorug05G0287900 Rorug05G0288700 Rorug05G0586900 Rorug06G0231700 Rorug06G0354300 Rorug06G0354400
rosa_samantha Rh1DG122200 Rh2DG686800 Rh5BG136600 Rh6AG344500 Rh6BG351900 Rh6CG359000 Rh6CG359100 Rh6CG387600 Rh6CG480500 Rh6DG344600 Rh6DG467400
rosa_wichuraiana Rw0G010250 Rw0G014820 Rw0G021400 Rw0G023100 Rw1G009450 Rw1G012100 Rw1G012180 Rw1G028360 Rw2G054260 Rw3G024370 Rw3G024420 Rw3G024470 Rw5G010850 Rw5G011010 Rw5G011020 Rw5G011050 Rw5G011080 Rw5G011120 Rw5G012300 Rw5G018470 Rw5G033830 Rw6G008930 Rw6G030070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 207
AasI GACNNNNNNGTC 1 cut(s) 245
Acc65I GGTACC 1 cut(s) 963
AccB1I GGYRCC 3 cut(s) 963, 1274, 1784
AccB7I CCANNNNNTGG 1 cut(s) 82
AccI GTMKAC 3 cut(s) 5, 1179, 1380
AclI AACGTT 1 cut(s) 2064
AclWI GGATC 3 cut(s) 881, 894, 1458
AcoI YGGCCR 1 cut(s) 1290
AcsI RAATTY 3 cut(s) 392, 610, 1641
AcuI CTGAAG 1 cut(s) 1716
AfaI GTAC 5 cut(s) 458, 965, 1172, 1375, 1588
AfiI CCNNNNNNNGG 5 cut(s) 82, 267, 332, 1561, 2014
AflII CTTAAG 2 cut(s) 1316, 2075
AflIII ACRYGT 2 cut(s) 672, 944
AgsI TTSAA 7 cut(s) 361, 477, 616, 658, 1028, 2088, 2110
AhlI ACTAGT 1 cut(s) 64
AjnI CCWGG 2 cut(s) 296, 438
Alw26I GTCTC 2 cut(s) 700, 1538
AlwI GGATC 3 cut(s) 881, 894, 1458
AlwNI CAGNNNCTG 1 cut(s) 1667
Ama87I CYCGRG 1 cut(s) 806
AoxI GGCC 2 cut(s) 1135, 1290
ApeKI GCWGC 8 cut(s) 31, 468, 582, 624, 744, 816, 1658, 2071
ApoI RAATTY 3 cut(s) 392, 610, 1641
AseI ATTAAT 5 cut(s) 873, 1151, 1476, 1503, 2052
Asp700I GAANNNNTTC 2 cut(s) 365, 1217
Asp718I GGTACC 1 cut(s) 963
AspLEI GCGC 3 cut(s) 200, 325, 1951
AspS9I GGNCC 2 cut(s) 1855, 2017
AsuHPI GGTGA 6 cut(s) 655, 904, 1294, 1531, 1570, 2044
AvaI CYCGRG 1 cut(s) 806
AvaII GGWCC 2 cut(s) 1855, 2017
BaeI ACNNNNGTAYC 2 cut(s) 240, 273
BamHI GGATCC 1 cut(s) 886
BanI GGYRCC 3 cut(s) 963, 1274, 1784
BanII GRGCYC 2 cut(s) 807, 1128
BarI GAAGNNNNNNTAC 2 cut(s) 1542, 1574
BbvI GCAGC 8 cut(s) 18, 455, 594, 636, 756, 803, 1645, 2083
BccI CCATC 6 cut(s) 260, 1182, 1718, 2033, 2170, 2330
BceAI ACGGC 2 cut(s) 507, 1305
BcgI CGANNNNNNTGC 2 cut(s) 302, 336
BciT130I CCWGG 2 cut(s) 298, 440
BciVI GTATCC 2 cut(s) 268, 2275
BclI TGATCA 1 cut(s) 2316
BcoDI GTCTC 2 cut(s) 700, 1538
BcuI ACTAGT 1 cut(s) 64
BfaI CTAG 2 cut(s) 65, 1985
BfmI CTRYAG 4 cut(s) 622, 1659, 1824, 1903
BfoI RGCGCY 1 cut(s) 1952
BfrI CTTAAG 2 cut(s) 1316, 2075
BfuI GTATCC 2 cut(s) 268, 2275
BisI GCNGC 8 cut(s) 32, 469, 583, 625, 745, 817, 1659, 2072
BlpI GCTNAGC 1 cut(s) 1815
BlsI GCNGC 8 cut(s) 33, 470, 584, 626, 746, 818, 1660, 2073
Bme1390I CCNGG 2 cut(s) 298, 440
Bme18I GGWCC 2 cut(s) 1855, 2017
BmeT110I CYCGRG 1 cut(s) 806
BmgT120I GGNCC 2 cut(s) 1855, 2017
BmiI GGNNCC 7 cut(s) 888, 965, 1276, 1786, 1800, 1857, 2312
BmrFI CCNGG 2 cut(s) 298, 440
BmsI GCATC 5 cut(s) 851, 908, 1008, 1167, 2194
BoxI GACNNNNGTC 1 cut(s) 1550
BpmI CTGGAG 2 cut(s) 1692, 1774
Bpu10I CCTNAGC 1 cut(s) 1410
Bpu1102I GCTNAGC 1 cut(s) 1815
BpuEI CTTGAG 3 cut(s) 270, 1190, 2180
BsaAI YACGTR 1 cut(s) 945
BsaBI GATNNNNATC 1 cut(s) 1755
BsaJI CCNNGG 3 cut(s) 297, 439, 2189
BsaWI WCCGGW 1 cut(s) 889
BsaXI ACNNNNNCTCC 4 cut(s) 650, 680, 2049, 2079
Bsc4I CCNNNNNNNGG 5 cut(s) 82, 267, 332, 1561, 2014
Bse1I ACTGG 5 cut(s) 707, 1566, 1879, 2256, 2327
Bse3DI GCAATG 2 cut(s) 127, 2157
Bse8I GATNNNNATC 1 cut(s) 1755
BseBI CCWGG 2 cut(s) 298, 440
BseDI CCNNGG 3 cut(s) 297, 439, 2189
BseGI GGATG 9 cut(s) 71, 796, 866, 1110, 1221, 1602, 1756, 2181, 2185
BseJI GATNNNNATC 1 cut(s) 1755
BseLI CCNNNNNNNGG 5 cut(s) 82, 267, 332, 1561, 2014
BseMI GCAATG 2 cut(s) 127, 2157
BseMII CTCAG 4 cut(s) 390, 713, 1829, 2159
BseNI ACTGG 5 cut(s) 707, 1566, 1879, 2256, 2327
BseRI GAGGAG 1 cut(s) 2234
BseXI GCAGC 8 cut(s) 18, 455, 594, 636, 756, 803, 1645, 2083
BshFI GGCC 2 cut(s) 1137, 1292
BshNI GGYRCC 3 cut(s) 963, 1274, 1784
BsiHKCI CYCGRG 1 cut(s) 806
BsiSI CCGG 2 cut(s) 890, 1967
BslFI GGGAC 2 cut(s) 1538, 2178
BslI CCNNNNNNNGG 5 cut(s) 82, 267, 332, 1561, 2014
BsmAI GTCTC 2 cut(s) 700, 1538
BsmFI GGGAC 2 cut(s) 1538, 2178
BsmI GAATGC 2 cut(s) 129, 884
BsnI GGCC 2 cut(s) 1137, 1292
BsoBI CYCGRG 1 cut(s) 806
Bsp1286I GDGCHC 2 cut(s) 807, 1128
Bsp1407I TGTACA 1 cut(s) 456
Bsp143I GATC 5 cut(s) 750, 886, 1450, 1807, 2316
Bsp1720I GCTNAGC 1 cut(s) 1815
Bsp19I CCATGG 1 cut(s) 2189
BspANI GGCC 2 cut(s) 1137, 1292
BspCNI CTCAG 4 cut(s) 389, 712, 1828, 2160
BspHI TCATGA 2 cut(s) 1197, 2239
BspLI GGNNCC 7 cut(s) 888, 965, 1276, 1786, 1800, 1857, 2312
BspMAI CTGCAG 2 cut(s) 626, 1663
BspPI GGATC 3 cut(s) 881, 894, 1458
BspT107I GGYRCC 3 cut(s) 963, 1274, 1784
BspTI CTTAAG 2 cut(s) 1316, 2075
BsrDI GCAATG 2 cut(s) 127, 2157
BsrGI TGTACA 1 cut(s) 456
BsrI ACTGG 5 cut(s) 707, 1566, 1879, 2256, 2327
BssECI CCNNGG 3 cut(s) 297, 439, 2189
BssMI GATC 5 cut(s) 750, 886, 1450, 1807, 2316
BssT1I CCWWGG 1 cut(s) 2189
Bst2UI CCWGG 2 cut(s) 298, 440
Bst4CI ACNGT 5 cut(s) 82, 571, 1378, 1828, 1843
BstAFI CTTAAG 2 cut(s) 1316, 2075
BstAPI GCANNNNNTGC 1 cut(s) 1667
BstAUI TGTACA 1 cut(s) 456
BstBAI YACGTR 1 cut(s) 945
BstC8I GCNNGC 1 cut(s) 952
BstDEI CTNAG 6 cut(s) 376, 699, 1410, 1815, 2009, 2168
BstDSI CCRYGG 1 cut(s) 2189
BstENI CCTNNNNNAGG 1 cut(s) 2012
BstF5I GGATG 9 cut(s) 71, 796, 866, 1110, 1221, 1602, 1756, 2181, 2185
BstH2I RGCGCY 1 cut(s) 1952
BstHHI GCGC 3 cut(s) 200, 325, 1951
BstKTI GATC 5 cut(s) 753, 889, 1453, 1810, 2319
BstMAI GTCTC 2 cut(s) 700, 1538
BstMBI GATC 5 cut(s) 750, 886, 1450, 1807, 2316
BstMWI GCNNNNNNNGC 6 cut(s) 40, 126, 582, 822, 1201, 1667
BstNI CCWGG 2 cut(s) 298, 440
BstNSI RCATGY 2 cut(s) 676, 768
BstPAI GACNNNNGTC 1 cut(s) 1550
BstSCI CCNGG 2 cut(s) 296, 438
BstSFI CTRYAG 4 cut(s) 622, 1659, 1824, 1903
BstV1I GCAGC 8 cut(s) 18, 455, 594, 636, 756, 803, 1645, 2083
BstX2I RGATCY 1 cut(s) 886
BstXI CCANNNNNNTGG 2 cut(s) 974, 1447
BstYI RGATCY 1 cut(s) 886
BsuI GTATCC 2 cut(s) 268, 2275
BsuRI GGCC 2 cut(s) 1137, 1292
BtgI CCRYGG 1 cut(s) 2189
BtgZI GCGATG 1 cut(s) 985
BtsCI GGATG 9 cut(s) 71, 796, 866, 1110, 1221, 1602, 1756, 2181, 2185
BtsIMutI CAGTG 5 cut(s) 714, 864, 1345, 1833, 2249
Cac8I GCNNGC 1 cut(s) 952
CaiI CAGNNNCTG 1 cut(s) 1667
CciI TCATGA 2 cut(s) 1197, 2239
CfoI GCGC 3 cut(s) 200, 325, 1951
Cfr13I GGNCC 2 cut(s) 1855, 2017
Csp6I GTAC 5 cut(s) 457, 964, 1171, 1374, 1587
CviQI GTAC 5 cut(s) 457, 964, 1171, 1374, 1587
DdeI CTNAG 6 cut(s) 376, 699, 1410, 1815, 2009, 2168
DpnI GATC 5 cut(s) 752, 888, 1452, 1809, 2318
DpnII GATC 5 cut(s) 750, 886, 1450, 1807, 2316
DrdI GACNNNNNNGTC 1 cut(s) 245
DseDI GACNNNNNNGTC 1 cut(s) 245
EaeI YGGCCR 1 cut(s) 1290
Eco130I CCWWGG 1 cut(s) 2189
Eco24I GRGCYC 2 cut(s) 807, 1128
Eco47I GGWCC 2 cut(s) 1855, 2017
Eco57I CTGAAG 1 cut(s) 1716
Eco88I CYCGRG 1 cut(s) 806
EcoNI CCTNNNNNAGG 1 cut(s) 2012
EcoRII CCWGG 2 cut(s) 296, 438
EcoT14I CCWWGG 1 cut(s) 2189
EcoT22I ATGCAT 3 cut(s) 766, 923, 2187
EcoT38I GRGCYC 2 cut(s) 807, 1128
ErhI CCWWGG 1 cut(s) 2189
FalI AAGNNNNNCTT 4 cut(s) 478, 510, 644, 676
FaqI GGGAC 2 cut(s) 1538, 2178
FbaI TGATCA 1 cut(s) 2316
FblI GTMKAC 3 cut(s) 5, 1179, 1380
Fnu4HI GCNGC 8 cut(s) 32, 469, 583, 625, 745, 817, 1659, 2072
FokI GGATG 9 cut(s) 58, 803, 873, 1117, 1208, 1589, 1763, 2172, 2188
FriOI GRGCYC 2 cut(s) 807, 1128
Fsp4HI GCNGC 8 cut(s) 32, 469, 583, 625, 745, 817, 1659, 2072
FspBI CTAG 2 cut(s) 65, 1985
GlaI GCGC 3 cut(s) 199, 324, 1950
GluI GCNGC 8 cut(s) 32, 469, 583, 625, 745, 817, 1659, 2072
GsuI CTGGAG 2 cut(s) 1692, 1774
HaeII RGCGCY 1 cut(s) 1952
HaeIII GGCC 2 cut(s) 1137, 1292
HapII CCGG 2 cut(s) 890, 1967
HhaI GCGC 3 cut(s) 200, 325, 1951
Hin6I GCGC 3 cut(s) 198, 323, 1949
HinP1I GCGC 3 cut(s) 198, 323, 1949
HincII GTYRAC 1 cut(s) 1884
HindII GTYRAC 1 cut(s) 1884
HindIII AAGCTT 2 cut(s) 1523, 1698
HinfI GANTC 6 cut(s) 314, 844, 986, 1350, 1622, 2207
HpaII CCGG 2 cut(s) 890, 1967
HphI GGTGA 6 cut(s) 655, 904, 1294, 1531, 1570, 2044
Hpy166II GTNNAC 7 cut(s) 6, 459, 1180, 1381, 1734, 1884, 1960
Hpy188I TCNGA 7 cut(s) 216, 379, 670, 1261, 1696, 1777, 1807
Hpy188III TCNNGA 9 cut(s) 287, 716, 754, 796, 1198, 1432, 1543, 1791, 2240
Hpy8I GTNNAC 7 cut(s) 6, 459, 1180, 1381, 1734, 1884, 1960
HpyAV CCTTC 7 cut(s) 824, 833, 1315, 1939, 2111, 2134, 2166
HpyCH4III ACNGT 5 cut(s) 82, 571, 1378, 1828, 1843
HpyCH4IV ACGT 4 cut(s) 240, 255, 944, 2064
HpyF10VI GCNNNNNNNGC 6 cut(s) 40, 126, 582, 822, 1201, 1667
HpyF3I CTNAG 6 cut(s) 376, 699, 1410, 1815, 2009, 2168
HpySE526I ACGT 4 cut(s) 240, 255, 944, 2064
HspAI GCGC 3 cut(s) 198, 323, 1949
KpnI GGTACC 1 cut(s) 967
Ksp22I TGATCA 1 cut(s) 2316
Kzo9I GATC 5 cut(s) 750, 886, 1450, 1807, 2316
LmnI GCTCC 3 cut(s) 1068, 2057, 2155
Lsp1109I GCAGC 8 cut(s) 18, 455, 594, 636, 756, 803, 1645, 2083
LweI GCATC 5 cut(s) 851, 908, 1008, 1167, 2194
MaeI CTAG 2 cut(s) 65, 1985
MaeII ACGT 4 cut(s) 240, 255, 944, 2064
MaeIII GTNAC 7 cut(s) 161, 236, 247, 410, 833, 1072, 1576
MalI GATC 5 cut(s) 752, 888, 1452, 1809, 2318
MboI GATC 5 cut(s) 750, 886, 1450, 1807, 2316
MboII GAAGA 3 cut(s) 218, 1210, 1291
MflI RGATCY 1 cut(s) 886
MhlI GDGCHC 2 cut(s) 807, 1128
MluCI AATT 8 cut(s) 392, 426, 505, 610, 1029, 1469, 1500, 1641
MlyI GAGTC 1 cut(s) 980
MmeI TCCRAC 4 cut(s) 693, 924, 1093, 1650
Mph1103I ATGCAT 3 cut(s) 766, 923, 2187
MroXI GAANNNNTTC 2 cut(s) 365, 1217
MslI CAYNNNNRTG 5 cut(s) 972, 1338, 1512, 1722, 2271
MspCI CTTAAG 2 cut(s) 1316, 2075
MspI CCGG 2 cut(s) 890, 1967
MspR9I CCNGG 2 cut(s) 298, 440
Mva1269I GAATGC 2 cut(s) 129, 884
MvaI CCWGG 2 cut(s) 298, 440
MwoI GCNNNNNNNGC 6 cut(s) 40, 126, 582, 822, 1201, 1667
NcoI CCATGG 1 cut(s) 2189
NdeII GATC 5 cut(s) 750, 886, 1450, 1807, 2316
NlaIV GGNNCC 7 cut(s) 888, 965, 1276, 1786, 1800, 1857, 2312
NmuCI GTSAC 4 cut(s) 236, 247, 410, 1576
NsiI ATGCAT 3 cut(s) 766, 923, 2187
NspI RCATGY 2 cut(s) 676, 768
PagI TCATGA 2 cut(s) 1197, 2239
PciI ACATGT 1 cut(s) 672
PcsI WCGNNNNNNNCGW 1 cut(s) 834
PctI GAATGC 2 cut(s) 129, 884
PdmI GAANNNNTTC 2 cut(s) 365, 1217
PfeI GAWTC 5 cut(s) 314, 844, 1350, 1622, 2207
PflMI CCANNNNNTGG 1 cut(s) 82
PkrI GCNGC 8 cut(s) 33, 470, 584, 626, 746, 818, 1660, 2073
PleI GAGTC 1 cut(s) 980
PpsI GAGTC 1 cut(s) 980
Ppu21I YACGTR 1 cut(s) 945
PscI ACATGT 1 cut(s) 672
PshAI GACNNNNGTC 1 cut(s) 1550
PshBI ATTAAT 5 cut(s) 873, 1151, 1476, 1503, 2052
PsiI TTATAA 1 cut(s) 207
Psp1406I AACGTT 1 cut(s) 2064
Psp6I CCWGG 2 cut(s) 296, 438
PspGI CCWGG 2 cut(s) 296, 438
PspN4I GGNNCC 7 cut(s) 888, 965, 1276, 1786, 1800, 1857, 2312
PspPI GGNCC 2 cut(s) 1855, 2017
PstI CTGCAG 2 cut(s) 626, 1663
PstNI CAGNNNCTG 1 cut(s) 1667
PsuI RGATCY 1 cut(s) 886
RsaI GTAC 5 cut(s) 458, 965, 1172, 1375, 1588
RsaNI GTAC 5 cut(s) 457, 964, 1171, 1374, 1587
RseI CAYNNNNRTG 5 cut(s) 972, 1338, 1512, 1722, 2271
SatI GCNGC 8 cut(s) 32, 469, 583, 625, 745, 817, 1659, 2072
Sau3AI GATC 5 cut(s) 750, 886, 1450, 1807, 2316
Sau96I GGNCC 2 cut(s) 1855, 2017
SchI GAGTC 1 cut(s) 980
ScrFI CCNGG 2 cut(s) 298, 440
SduI GDGCHC 2 cut(s) 807, 1128
SfaNI GCATC 5 cut(s) 851, 908, 1008, 1167, 2194
SfcI CTRYAG 4 cut(s) 622, 1659, 1824, 1903
SinI GGWCC 2 cut(s) 1855, 2017
SmiMI CAYNNNNRTG 5 cut(s) 972, 1338, 1512, 1722, 2271
SmlI CTYRAG 5 cut(s) 285, 1205, 1316, 2075, 2159
SmoI CTYRAG 5 cut(s) 285, 1205, 1316, 2075, 2159
SpeI ACTAGT 1 cut(s) 64
Sse9I AATT 8 cut(s) 392, 426, 505, 610, 1029, 1469, 1500, 1641
SspI AATATT 1 cut(s) 1020
SspMI CTAG 2 cut(s) 65, 1985
StyD4I CCNGG 2 cut(s) 296, 438
StyI CCWWGG 1 cut(s) 2189
TaaI ACNGT 5 cut(s) 82, 571, 1378, 1828, 1843
TaiI ACGT 4 cut(s) 243, 258, 947, 2067
TaqI TCGA 6 cut(s) 229, 717, 725, 1453, 1542, 1596
TaqII GACCGA 1 cut(s) 2034
TasI AATT 8 cut(s) 392, 426, 505, 610, 1029, 1469, 1500, 1641
TatI WGTACW 2 cut(s) 456, 1170
TfiI GAWTC 5 cut(s) 314, 844, 1350, 1622, 2207
TscAI CASTG 5 cut(s) 714, 871, 1345, 1833, 2256
TseFI GTSAC 4 cut(s) 236, 247, 410, 1576
TseI GCWGC 8 cut(s) 31, 468, 582, 624, 744, 816, 1658, 2071
Tsp45I GTSAC 4 cut(s) 236, 247, 410, 1576
TspGWI ACGGA 3 cut(s) 129, 614, 1791
TspRI CASTG 5 cut(s) 714, 871, 1345, 1833, 2256
Van91I CCANNNNNTGG 1 cut(s) 82
Vha464I CTTAAG 2 cut(s) 1316, 2075
VpaK11BI GGWCC 2 cut(s) 1855, 2017
VspI ATTAAT 5 cut(s) 873, 1151, 1476, 1503, 2052
XagI CCTNNNNNAGG 1 cut(s) 2012
XapI RAATTY 3 cut(s) 392, 610, 1641
XceI RCATGY 2 cut(s) 676, 768
XcmI CCANNNNNNNNNTGG 2 cut(s) 113, 2143
XmiI GTMKAC 3 cut(s) 5, 1179, 1380
XmnI GAANNNNTTC 2 cut(s) 365, 1217
XspI CTAG 2 cut(s) 65, 1985
Zsp2I ATGCAT 3 cut(s) 766, 923, 2187
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.