Rroxscaffold_1G00060040

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000066
Physical Location & Seq
Forward (+)
82168890 .. 82173479
4590 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00060040.1

Sequence Viewer

Length: 2172 bp
ATGGCCTCATTACATGAGAGGATTCTTGTTACGCAGCTCTATTTGTTAATGTTAGTGGCCGCAACAACAACATCAACAACACTGCTAGCAGCAGCGCAGACCCTACCGGCGCCTCCTCAAGCCAAGTCTGGGTGCAAAGAGAAGTGTGGTGATCTCACAATTCCATATCCATTTGGCATAGGTGAAAATTGTTCCATGCGACCGGAATTCACCATCGCTTGTGACCAATCCACCACTGTATCCCCATCGGCAAACTTCACAAATGGTACCTTCACCTTTCCCATCACCATTACTAAATTTTCCCTTGATGACGGCGAGTTGCAAGTAATGCAGGACATAGCACGAAATTGTTATGATGACAACGGTACTGAAACAAACAGCGGCAGTTTACTGGAGCTGCCTGCTCCTTATATCATCTCAACCAAAAACAACTTCTTTACCGTTGGCTGCAACGTTTTATCGCTGTATCAACAAGTTGTAGATGAAGACCACCAATCAGGTGTAAGTGTTCCCTTATGTGCGGATATTCTTGGCAAAGAAATACCCGAGTCTTGCAACGGCTTCGGCTGTGCCCAAACTTCCATCCCAAGTGGACTGCAAAACATTTCGATTGCAATGGGCCAACTTTACAGCTATAACGCTAGTGATCCCTGGGCATCAAACTACAAGTGCAGCTACGCCTTCATCGTTGAAGAAGGCAACTTCACGTTCGCTCCGAAAACAAGTTTTCAACAACTGAATACAACAAATCAACAGCTTCCGGTGGCTGTTAATTGGGCAATCGGGAACGAGCCATGTGAAGTAGCAAAGAACCTGACTAATTATTCGTGCAAACAGAATACTACTTGTGTCGACCGGAGTACCATCACTAATGAGACGACTGCAGGTTACATTTGCCAGTGCCTGCCAGGCTACGAAGGGAACCCATACCTCGGTTGCCGAGGTGTCAGTATAGGCTTCTTAGTTTTGGTGGTCTTAATTTCGTGGATATATTGGGAAATGAACAAAAGAAGGCTCATCAAACTCAAAAAGAAGTACTTCAAAGAAAACGGTGGCTTAACATTACAACAACAACTCGCTAGTCATGGAGGAGCCGTGGAAACAACAAAAATCTTTACAGCGGAAGACCTTGAGAAGGCGACAAACAATTACCATGCAGATGAGATTCTTGGTGAAGGAGCCTATGGAACAGTTTACAAAGGAATTCTACGTGATAAGAAAGTTGTTGCCATAAAAAAATCAAAAATTGGCGCCCCAACTCAAAGTGAGCAGTTCGTTAATGAGGTTATCGTTCTCTCTCAAATCAACCACAGAAACGTAGTAAAACTATTGGGTTGTTGTTTCGAGACTGAAGTGCCTTTACTAGTTTATGAGTACATTACCCATGGCACTCTTTTTGAGCATATATTCAGTAAGGGAAAAGGATCACCGCTTTCATGGGAATTACGATTGAAGATAGCATCAGAAACGGCAGGGGCACTAGCTTACTTACACTCCTCTACTTCCACACCAATCATACATCGAGACGTGAAAGCAACAAATATACTATTAGATGACAATTACACGGCCAAAGTGTCCGATTTTGGAGCTTCGCGTTTCATTCCTCTAGATCAAACTGAACTAGCAACACTAGTGCAAGGGACGCTTGGCTACTTGGACCCTGAATACTTTCATTCCAATCAACTGACTGAAAAGAGTGACGTCTACAGCTTTGGAGTTGTCCTAGCAGAGTTATTAACGAGCAGAGTGGCACTTTCTTTCGCTAGACCTGACGCAGAGAGATGCCTAGCAAGTTTCTTTGTTTCTTCAATAGAGAAAGATTGCTTGATTGAAATTCTTGACGCTGACATAGTGAATGAGGGGAACATAGACACAGCAGAGCAAGTTGCTTATCTCGCAAAAAGATGTCTAGGGATAAGAGGGGAGGAAAGGCCTTCAATGAAAGAAGTGGCCATGGAGTTGGTGGGAATGCAAAATACAGCGAAGCATCCATGGCGAAATAATGCAAATTTATGTCCCGAGGAGACTGAGTACTTGCTGGGGTCACCTATCAATCCAGAAGCTTACATTGTGAATGTTAAAGGCGATGGTGATGAAGGTTCTAGTGGTACAACCAGTGGGTATGACAGCATGCAAATTCAAATGTTAATGCCATATGATGATGCGCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK hEGF

Protein Analysis

723

Amino Acids

79.38

Weight (kDa)

4.96

Isoelectric Point (pI)

32.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 45 - 109 3.8e-09 Wall-associated receptor kinase galacturonan-binding
Pkinase PF00069 384 - 651 1.1e-45 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 386 - 654 6.3e-47 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21210 AT1G21230 AT1G21240 AT1G21240 AT1G21245 AT1G21250 AT1G21270 AT1G22720 AT3G25490
fragaria_vesca FvH4_2g35051 FvH4_2g35051 FvH4_2g35060 FvH4_3g10281 FvH4_3g10285 FvH4_3g10286 FvH4_3g10311 FvH4_3g10311 FvH4_3g10320 FvH4_3g10332 FvH4_3g10340 FvH4_3g10350 FvH4_3g10360 FvH4_3g10390 FvH4_3g10412 FvH4_3g10413 FvH4_3g10461 FvH4_3g10462 FvH4_3g10470 FvH4_3g10510 FvH4_3g10520 FvH4_3g10530 FvH4_3g10550 FvH4_3g10560 FvH4_3g10611 FvH4_3g11730 FvH4_6g53140 FvH4_6g53142 FvH4_7g31880 FvH4_7g31910 FvH4_7g31920
malus_domestica MD05G1269900.v1.1 MD05G1270000.v1.1 MD05G1270200.v1.1 MD05G1270400.v1.1 MD05G1270500.v1.1 MD05G1270600.v1.1 MD05G1270800.v1.1 MD05G1270900.v1.1 MD07G1183800.v1.1 MD08G1107600.v1.1 MD10G1248500.v1.1 MD10G1249200.v1.1 MD10G1249500.v1.1 MD10G1250000.v1.1 MD10G1250500.v1.1 MD10G1250900.v1.1 MD10G1251200.v1.1 MD10G1251400.v1.1 MD13G1004600.v1.1 MD15G1088400.v1.1
prunus_persica Prupe.1G442400_v2.0.a1 Prupe.1G442500_v2.0.a1 Prupe.2G223400_v2.0.a1 Prupe.4G091900_v2.0.a1 Prupe.4G093200_v2.0.a1 Prupe.4G093300_v2.0.a1 Prupe.4G093400_v2.0.a1 Prupe.4G093500_v2.0.a1 Prupe.4G094100_v2.0.a1 Prupe.4G094200_v2.0.a1 Prupe.4G094300_v2.0.a1 Prupe.6G358300_v2.0.a1
pyrus_communis pycom08g08990 pycom10g20660 pycom10g20670 pycom10g20680 pycom10g20710 pycom10g20740 pycom13g00400 pycom15g08290
rosa_chinensis RchiOBHm_Chr1g0326861 RchiOBHm_Chr1g0326871 RchiOBHm_Chr1g0331391 RchiOBHm_Chr1g0336911 RchiOBHm_Chr1g0364001 RchiOBHm_Chr2g0174541 RchiOBHm_Chr2g0175041 RchiOBHm_Chr2g0175101 RchiOBHm_Chr2g0175121 RchiOBHm_Chr3g0486331 RchiOBHm_Chr5g0015581 RchiOBHm_Chr5g0016551 RchiOBHm_Chr5g0016561 RchiOBHm_Chr5g0016581 RchiOBHm_Chr5g0016601 RchiOBHm_Chr5g0016611 RchiOBHm_Chr5g0016641 RchiOBHm_Chr5g0016661 RchiOBHm_Chr5g0016691 RchiOBHm_Chr5g0016701 RchiOBHm_Chr5g0016731 RchiOBHm_Chr5g0016741 RchiOBHm_Chr5g0016811 RchiOBHm_Chr5g0016841 RchiOBHm_Chr5g0016871 RchiOBHm_Chr5g0016881 RchiOBHm_Chr5g0016931 RchiOBHm_Chr5g0017021 RchiOBHm_Chr5g0017041 RchiOBHm_Chr5g0017071 RchiOBHm_Chr5g0017091 RchiOBHm_Chr5g0017121 RchiOBHm_Chr5g0017141 RchiOBHm_Chr5g0017291 RchiOBHm_Chr5g0017321 RchiOBHm_Chr5g0017401 RchiOBHm_Chr5g0019001 RchiOBHm_Chr5g0019071 RchiOBHm_Chr5g0028941 RchiOBHm_Chr5g0052141 RchiOBHm_Chr5g0054721 RchiOBHm_Chr5g0054801 RchiOBHm_Chr5g0054931 RchiOBHm_Chr5g0054941 RchiOBHm_Chr6g0259291 RchiOBHm_Chr6g0292991 RchiOBHm_Chr6g0306911
rosa_laevigata RLG00000009465 RLG00000009468 RLG00000010755 RLG00000011976 RLG00000014541 RLG00000014542 RLG00000015169 RLG00000022308 RLG00000027529 RLG00000029324 RLG00000030244 RLG00000030245 RLG00000032210 RLG00000032211 RLG00000032257 RLG00000032259 RLG00000032260 RLG00000032261 RLG00000032262 RLG00000032268 RLG00000032269 RLG00000032272 RLG00000032282 RLG00000032283 RLG00000032308 RLG00000032413 RLG00000033129 RLG00000034945
rosa_multiflora Rmu_co8241929.1_g000001 Rmu_co8269227.1_g000001 Rmu_co8461259.1_g000001 Rmu_co8495385.1_g000001 Rmu_sc0000028.1_g000003 Rmu_sc0000083.1_g000009 Rmu_sc0000083.1_g000010 Rmu_sc0000083.1_g000031 Rmu_sc0000083.1_g000043 Rmu_sc0000083.1_g000046 Rmu_sc0000083.1_g000047 Rmu_sc0000563.1_g000024 Rmu_sc0000842.1_g000012 Rmu_sc0001235.1_g000001 Rmu_sc0001235.1_g000005 Rmu_sc0001235.1_g000009 Rmu_sc0001235.1_g000015 Rmu_sc0001235.1_g000016 Rmu_sc0001235.1_g000017 Rmu_sc0001473.1_g000036 Rmu_sc0001607.1_g000016 Rmu_sc0001788.1_g000006 Rmu_sc0002018.1_g000008 Rmu_sc0002028.1_g000001 Rmu_sc0002680.1_g000011 Rmu_sc0002798.1_g000023 Rmu_sc0002886.1_g000023 Rmu_sc0002989.1_g000012 Rmu_sc0003064.1_g000009 Rmu_sc0003382.1_g000006 Rmu_sc0003630.1_g000002 Rmu_sc0003630.1_g000047 Rmu_sc0003630.1_g000066 Rmu_sc0003630.1_g000067 Rmu_sc0003649.1_g000010 Rmu_sc0003688.1_g000005 Rmu_sc0003920.1_g000007 Rmu_sc0004765.1_g000007 Rmu_sc0004765.1_g000008 Rmu_sc0004920.1_g000011 Rmu_sc0005223.1_g000008 Rmu_sc0005223.1_g000010 Rmu_sc0005223.1_g000015 Rmu_sc0005223.1_g000016 Rmu_sc0005319.1_g000014 Rmu_sc0005319.1_g000015 Rmu_sc0005319.1_g000018 Rmu_sc0005319.1_g000019 Rmu_sc0005319.1_g000025 Rmu_sc0005418.1_g000007 Rmu_sc0005418.1_g000012 Rmu_sc0006106.1_g000011 Rmu_sc0006914.1_g000012 Rmu_sc0007023.1_g000001 Rmu_sc0007662.1_g000001 Rmu_sc0007920.1_g000002 Rmu_sc0007920.1_g000013 Rmu_sc0008053.1_g000016 Rmu_sc0008053.1_g000017 Rmu_sc0008053.1_g000018 Rmu_sc0008053.1_g000023 Rmu_sc0008053.1_g000050 Rmu_sc0009042.1_g000007 Rmu_sc0009042.1_g000008 Rmu_sc0010642.1_g000008 Rmu_sc0011109.1_g000002 Rmu_sc0011314.1_g000006 Rmu_sc0011974.1_g000006 Rmu_sc0012179.1_g000009 Rmu_sc0015169.1_g000001 Rmu_sc0016368.1_g000002 Rmu_sc0022659.1_g000001 Rmu_sc0027371.1_g000001 Rmu_sc0028213.1_g000001 Rmu_sc0034526.1_g000001 Rmu_sc0039366.1_g000002 Rmu_ssc0000050.1_g000084 Rmu_ssc0000050.1_g000088 Rmu_ssc0000167.1_g000017
rosa_roxburghii Rroxscaffold_1G00011340 Rroxscaffold_1G00025710 Rroxscaffold_1G00025870 Rroxscaffold_1G00026070 Rroxscaffold_1G00026170 Rroxscaffold_1G00026200 Rroxscaffold_1G00026270 Rroxscaffold_1G00050950 Rroxscaffold_1G00058900 Rroxscaffold_1G00060040 Rroxscaffold_1G00060060 Rroxscaffold_1G00060070 Rroxscaffold_1G00060080 Rroxscaffold_1G00060110 Rroxscaffold_1G00060120 Rroxscaffold_1G00060140 Rroxscaffold_1G00060170 Rroxscaffold_1G00060210 Rroxscaffold_1G00060220 Rroxscaffold_1G00060240 Rroxscaffold_1G00060250 Rroxscaffold_1G00060260 Rroxscaffold_1G00060300 Rroxscaffold_1G00060320 Rroxscaffold_1G00060330 Rroxscaffold_1G00060900 Rroxscaffold_1G00060920 Rroxscaffold_1G00073980 Rroxscaffold_2G00077560 Rroxscaffold_2G00077580 Rroxscaffold_2G00146990 Rroxscaffold_3G00236200 Rroxscaffold_3G00236210 Rroxscaffold_3G00236220 Rroxscaffold_4G00292760 Rroxscaffold_4G00315200 Rroxscaffold_4G00315240 Rroxscaffold_4G00326240 Rroxscaffold_4G00328980 Rroxscaffold_5G00341890 Rroxscaffold_7G00161350 Rroxscaffold_7G00174490 Rroxscaffold_7G00206550
rosa_rugosa Rorug01G0093000 Rorug01G0093100 Rorug01G0128800 Rorug01G0312000 Rorug01G0312000 Rorug02G0581100 Rorug03G0224600 Rorug05G0028500 Rorug05G0032700 Rorug05G0032800 Rorug05G0032900 Rorug05G0032900 Rorug05G0033000 Rorug05G0033100 Rorug05G0033300 Rorug05G0033400 Rorug05G0033900 Rorug05G0034900 Rorug05G0035000 Rorug05G0046900 Rorug05G0047500 Rorug05G0047500 Rorug05G0111600 Rorug05G0287900 Rorug05G0288700 Rorug05G0586900 Rorug06G0231700 Rorug06G0354300 Rorug06G0354400
rosa_samantha Rh1DG122200 Rh2DG686800 Rh5BG136600 Rh6AG344500 Rh6BG351900 Rh6CG359000 Rh6CG359100 Rh6CG387600 Rh6CG480500 Rh6DG344600 Rh6DG467400
rosa_wichuraiana Rw0G010250 Rw0G014820 Rw0G021400 Rw0G023100 Rw1G009450 Rw1G012100 Rw1G012180 Rw1G028360 Rw2G054260 Rw3G024370 Rw3G024420 Rw3G024470 Rw5G010850 Rw5G011010 Rw5G011020 Rw5G011050 Rw5G011080 Rw5G011120 Rw5G012300 Rw5G018470 Rw5G033830 Rw6G008930 Rw6G030070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1704
Acc36I ACCTGC 1 cut(s) 875
Acc65I GGTACC 1 cut(s) 266
AccB1I GGYRCC 3 cut(s) 109, 266, 1250
AccI GTMKAC 2 cut(s) 852, 1704
AccII CGCG 2 cut(s) 1594, 2167
AciI CCGC 5 cut(s) 60, 381, 521, 1121, 1430
AclI AACGTT 1 cut(s) 453
AclWI GGATC 2 cut(s) 641, 1432
AcoI YGGCCR 3 cut(s) 57, 1566, 1950
AcsI RAATTY 6 cut(s) 206, 296, 1203, 1833, 2008, 2136
AcuI CTGAAG 1 cut(s) 1371
AcyI GRCGYC 3 cut(s) 110, 1251, 1701
AfaI GTAC 7 cut(s) 268, 367, 862, 1037, 1376, 2033, 2110
AfiI CCNNNNNNNGG 3 cut(s) 129, 932, 1135
AgsI TTSAA 8 cut(s) 692, 731, 1042, 1453, 1809, 1832, 1938, 2141
AhlI ACTAGT 2 cut(s) 1363, 1630
AjiI CACGTC 1 cut(s) 1528
AjnI CCWGG 2 cut(s) 650, 907
AjuI GAANNNNNNNTTGG 2 cut(s) 416, 448
AluBI AGCT 9 cut(s) 37, 397, 633, 675, 757, 1484, 1589, 1710, 2063
AluI AGCT 9 cut(s) 37, 397, 633, 675, 757, 1484, 1589, 1710, 2063
Alw26I GTCTC 4 cut(s) 869, 1340, 1518, 2018
AlwI GGATC 2 cut(s) 641, 1432
AlwNI CAGNNNCTG 1 cut(s) 904
Ama87I CYCGRG 2 cut(s) 545, 2018
AoxI GGCC 6 cut(s) 3, 57, 619, 1566, 1931, 1950
ApeKI GCWGC 6 cut(s) 34, 89, 92, 397, 447, 672
ApoI RAATTY 6 cut(s) 206, 296, 1203, 1833, 2008, 2136
ArsI GACNNNNNNTTYG 2 cut(s) 808, 840
Asp700I GAANNNNTTC 2 cut(s) 1037, 1668
Asp718I GGTACC 1 cut(s) 266
AspLEI GCGC 4 cut(s) 97, 112, 1253, 2167
AspS9I GGNCC 2 cut(s) 619, 1657
AsuHPI GGTGA 9 cut(s) 161, 194, 202, 265, 277, 1184, 1419, 2037, 2102
AsuNHI GCTAGC 1 cut(s) 85
AvaI CYCGRG 2 cut(s) 545, 2018
AvaII GGWCC 1 cut(s) 1657
BaeGI GKGCMC 2 cut(s) 574, 1480
BaeI ACNNNNGTAYC 4 cut(s) 222, 255, 844, 877
BalI TGGCCA 1 cut(s) 1952
BanI GGYRCC 3 cut(s) 109, 266, 1250
BarI GAAGNNNNNNTAC 2 cut(s) 1344, 1376
BbsI GAAGAC 2 cut(s) 492, 1131
BbvI GCAGC 6 cut(s) 46, 101, 104, 384, 434, 684
BccI CCATC 6 cut(s) 221, 253, 290, 590, 872, 2081
BceAI ACGGC 5 cut(s) 328, 574, 1079, 1485, 1581
BcgI CGANNNNNNTGC 2 cut(s) 544, 578
BciT130I CCWGG 2 cut(s) 652, 909
BciVI GTATCC 1 cut(s) 250
BcoDI GTCTC 4 cut(s) 869, 1340, 1518, 2018
BcuI ACTAGT 2 cut(s) 1363, 1630
BfmI CTRYAG 2 cut(s) 882, 1705
BfoI RGCGCY 2 cut(s) 113, 1254
BfuAI ACCTGC 1 cut(s) 875
BfuI GTATCC 1 cut(s) 250
BglI GCCNNNNNGGC 1 cut(s) 909
BisI GCNGC 8 cut(s) 35, 60, 90, 93, 382, 398, 448, 673
BlsI GCNGC 8 cut(s) 36, 61, 91, 94, 383, 399, 449, 674
BmcAI AGTACT 2 cut(s) 1037, 2033
Bme1390I CCNGG 2 cut(s) 652, 909
Bme18I GGWCC 1 cut(s) 1657
BmeT110I CYCGRG 2 cut(s) 545, 2018
BmgBI CACGTC 1 cut(s) 1528
BmgT120I GGNCC 2 cut(s) 619, 1657
BmiI GGNNCC 7 cut(s) 111, 268, 923, 1093, 1180, 1252, 1659
BmrFI CCNGG 2 cut(s) 652, 909
BmsI GCATC 5 cut(s) 665, 1469, 1772, 1996, 2152
BmtI GCTAGC 1 cut(s) 89
BpiI GAAGAC 2 cut(s) 492, 1131
BpmI CTGGAG 1 cut(s) 413
BpuEI CTTGAG 2 cut(s) 102, 1151
BsaAI YACGTR 1 cut(s) 1211
BsaHI GRCGYC 3 cut(s) 110, 1251, 1701
BsaJI CCNNGG 9 cut(s) 650, 651, 931, 940, 1095, 1384, 1953, 1991, 2019
BsaWI WCCGGW 3 cut(s) 202, 760, 855
BsaXI ACNNNNNCTCC 8 cut(s) 697, 727, 1080, 1110, 1478, 1508, 2015, 2045
Bsc4I CCNNNNNNNGG 3 cut(s) 129, 932, 1135
Bse118I RCCGGY 1 cut(s) 106
Bse1I ACTGG 3 cut(s) 396, 898, 2115
Bse3DI GCAATG 1 cut(s) 621
BseBI CCWGG 2 cut(s) 652, 909
BseDI CCNNGG 9 cut(s) 650, 651, 931, 940, 1095, 1384, 1953, 1991, 2019
BseGI GGATG 2 cut(s) 582, 1987
BseLI CCNNNNNNNGG 3 cut(s) 129, 932, 1135
BseMI GCAATG 1 cut(s) 621
BseMII CTCAG 1 cut(s) 2019
BseNI ACTGG 3 cut(s) 396, 898, 2115
BseRI GAGGAG 4 cut(s) 105, 1104, 1486, 2036
BseSI GKGCMC 2 cut(s) 574, 1480
BseXI GCAGC 6 cut(s) 46, 101, 104, 384, 434, 684
BseYI CCCAGC 1 cut(s) 2038
BsgI GTGCAG 1 cut(s) 691
Bsh1236I CGCG 2 cut(s) 1594, 2167
Bsh1285I CGRYCG 2 cut(s) 203, 856
BshFI GGCC 6 cut(s) 5, 59, 621, 1568, 1933, 1952
BshNI GGYRCC 3 cut(s) 109, 266, 1250
BsiEI CGRYCG 2 cut(s) 203, 856
BsiHKCI CYCGRG 2 cut(s) 545, 2018
BsiSI CCGG 4 cut(s) 107, 203, 761, 856
BslFI GGGAC 2 cut(s) 1654, 2001
BslI CCNNNNNNNGG 3 cut(s) 129, 932, 1135
BsmAI GTCTC 4 cut(s) 869, 1340, 1518, 2018
BsmBI CGTCTC 2 cut(s) 869, 1518
BsmFI GGGAC 2 cut(s) 1654, 2001
BsmI GAATGC 1 cut(s) 1974
BsnI GGCC 6 cut(s) 5, 59, 621, 1568, 1933, 1952
BsoBI CYCGRG 2 cut(s) 545, 2018
Bsp1286I GDGCHC 2 cut(s) 574, 1480
Bsp143I GATC 4 cut(s) 151, 646, 1424, 1609
Bsp19I CCATGG 3 cut(s) 1384, 1953, 1991
BspACI CCGC 5 cut(s) 60, 381, 521, 1121, 1430
BspANI GGCC 6 cut(s) 5, 59, 621, 1568, 1933, 1952
BspCNI CTCAG 1 cut(s) 2020
BspFNI CGCG 2 cut(s) 1594, 2167
BspLI GGNNCC 7 cut(s) 111, 268, 923, 1093, 1180, 1252, 1659
BspMAI CTGCAG 1 cut(s) 886
BspMI ACCTGC 1 cut(s) 875
BspOI GCTAGC 1 cut(s) 89
BspPI GGATC 2 cut(s) 641, 1432
BspT107I GGYRCC 3 cut(s) 109, 266, 1250
BsrDI GCAATG 1 cut(s) 621
BsrFI RCCGGY 1 cut(s) 106
BsrI ACTGG 3 cut(s) 396, 898, 2115
BssAI RCCGGY 1 cut(s) 106
BssECI CCNNGG 9 cut(s) 650, 651, 931, 940, 1095, 1384, 1953, 1991, 2019
BssMI GATC 4 cut(s) 151, 646, 1424, 1609
BssNI GRCGYC 3 cut(s) 110, 1251, 1701
BssT1I CCWWGG 3 cut(s) 1384, 1953, 1991
Bst2UI CCWGG 2 cut(s) 652, 909
Bst4CI ACNGT 5 cut(s) 238, 365, 442, 1052, 1192
BstACI GRCGYC 3 cut(s) 110, 1251, 1701
BstAPI GCANNNNNTGC 1 cut(s) 328
BstBAI YACGTR 1 cut(s) 1211
BstC8I GCNNGC 4 cut(s) 87, 402, 905, 2132
BstDEI CTNAG 2 cut(s) 961, 2028
BstDSI CCRYGG 4 cut(s) 1095, 1384, 1953, 1991
BstEII GGTNACC 1 cut(s) 2043
BstENI CCTNNNNNAGG 1 cut(s) 1133
BstF5I GGATG 2 cut(s) 582, 1987
BstFNI CGCG 2 cut(s) 1594, 2167
BstH2I RGCGCY 2 cut(s) 113, 1254
BstHHI GCGC 4 cut(s) 97, 112, 1253, 2167
BstKTI GATC 4 cut(s) 154, 649, 1427, 1612
BstMAI GTCTC 4 cut(s) 869, 1340, 1518, 2018
BstMBI GATC 4 cut(s) 151, 646, 1424, 1609
BstMCI CGRYCG 2 cut(s) 203, 856
BstMWI GCNNNNNNNGC 5 cut(s) 328, 909, 1642, 1895, 1993
BstNI CCWGG 2 cut(s) 652, 909
BstNSI RCATGY 1 cut(s) 2134
BstPI GGTNACC 1 cut(s) 2043
BstSCI CCNGG 2 cut(s) 650, 907
BstSFI CTRYAG 2 cut(s) 882, 1705
BstSLI GKGCMC 2 cut(s) 574, 1480
BstUI CGCG 2 cut(s) 1594, 2167
BstV1I GCAGC 6 cut(s) 46, 101, 104, 384, 434, 684
BstV2I GAAGAC 2 cut(s) 492, 1131
BstXI CCANNNNNNTGG 1 cut(s) 1960
BsuI GTATCC 1 cut(s) 250
BsuRI GGCC 6 cut(s) 5, 59, 621, 1568, 1933, 1952
BtgI CCRYGG 4 cut(s) 1095, 1384, 1953, 1991
BtgZI GCGATG 2 cut(s) 199, 2100
BtrI CACGTC 1 cut(s) 1528
BtsCI GGATG 2 cut(s) 582, 1987
BtsI GCAGTG 1 cut(s) 80
BtsIMutI CAGTG 4 cut(s) 80, 234, 905, 2122
BveI ACCTGC 1 cut(s) 875
Cac8I GCNNGC 4 cut(s) 87, 402, 905, 2132
CaiI CAGNNNCTG 1 cut(s) 904
CfoI GCGC 4 cut(s) 97, 112, 1253, 2167
Cfr10I RCCGGY 1 cut(s) 106
Cfr13I GGNCC 2 cut(s) 619, 1657
CseI GACGC 3 cut(s) 1651, 1781, 1850
Csp6I GTAC 7 cut(s) 267, 366, 861, 1036, 1375, 2032, 2109
CviQI GTAC 7 cut(s) 267, 366, 861, 1036, 1375, 2032, 2109
DdeI CTNAG 2 cut(s) 961, 2028
DinI GGCGCC 2 cut(s) 111, 1252
DpnI GATC 4 cut(s) 153, 648, 1426, 1611
DpnII GATC 4 cut(s) 151, 646, 1424, 1609
EaeI YGGCCR 3 cut(s) 57, 1566, 1950
Eco130I CCWWGG 3 cut(s) 1384, 1953, 1991
Eco147I AGGCCT 1 cut(s) 1933
Eco47I GGWCC 1 cut(s) 1657
Eco57I CTGAAG 1 cut(s) 1371
Eco88I CYCGRG 2 cut(s) 545, 2018
Eco91I GGTNACC 1 cut(s) 2043
EcoNI CCTNNNNNAGG 1 cut(s) 1133
EcoO65I GGTNACC 1 cut(s) 2043
EcoRI GAATTC 2 cut(s) 206, 1203
EcoRII CCWGG 2 cut(s) 650, 907
EcoT14I CCWWGG 3 cut(s) 1384, 1953, 1991
EgeI GGCGCC 2 cut(s) 111, 1252
EheI GGCGCC 2 cut(s) 111, 1252
ErhI CCWWGG 3 cut(s) 1384, 1953, 1991
Esp3I CGTCTC 2 cut(s) 869, 1518
FalI AAGNNNNNCTT 4 cut(s) 1022, 1054, 1629, 1661
FaqI GGGAC 2 cut(s) 1654, 2001
FauNDI CATATG 1 cut(s) 2155
FblI GTMKAC 2 cut(s) 852, 1704
Fnu4HI GCNGC 8 cut(s) 35, 60, 90, 93, 382, 398, 448, 673
FokI GGATG 2 cut(s) 569, 1974
Fsp4HI GCNGC 8 cut(s) 35, 60, 90, 93, 382, 398, 448, 673
GlaI GCGC 4 cut(s) 96, 111, 1252, 2166
GluI GCNGC 8 cut(s) 35, 60, 90, 93, 382, 398, 448, 673
GsaI CCCAGC 1 cut(s) 2042
GsuI CTGGAG 1 cut(s) 413
HaeII RGCGCY 2 cut(s) 113, 1254
HaeIII GGCC 6 cut(s) 5, 59, 621, 1568, 1933, 1952
HapII CCGG 4 cut(s) 107, 203, 761, 856
HgaI GACGC 3 cut(s) 1651, 1781, 1850
HhaI GCGC 4 cut(s) 97, 112, 1253, 2167
Hin1I GRCGYC 3 cut(s) 110, 1251, 1701
Hin6I GCGC 4 cut(s) 95, 110, 1251, 2165
HinP1I GCGC 4 cut(s) 95, 110, 1251, 2165
HincII GTYRAC 1 cut(s) 853
HindII GTYRAC 1 cut(s) 853
HindIII AAGCTT 1 cut(s) 2061
HinfI GANTC 3 cut(s) 22, 548, 1165
HpaII CCGG 4 cut(s) 107, 203, 761, 856
HphI GGTGA 9 cut(s) 161, 194, 202, 265, 277, 1184, 1419, 2037, 2102
Hpy166II GTNNAC 5 cut(s) 389, 593, 853, 1195, 1705
Hpy188I TCNGA 3 cut(s) 717, 1465, 1579
Hpy188III TCNNGA 7 cut(s) 784, 1345, 1523, 1607, 1838, 2018, 2057
Hpy8I GTNNAC 5 cut(s) 389, 593, 853, 1195, 1705
HpyAV CCTTC 9 cut(s) 280, 689, 691, 911, 1005, 1129, 1169, 1944, 2090
HpyCH4III ACNGT 5 cut(s) 238, 365, 442, 1052, 1192
HpyCH4IV ACGT 6 cut(s) 453, 707, 1210, 1317, 1527, 1701
HpyF10VI GCNNNNNNNGC 5 cut(s) 328, 909, 1642, 1895, 1993
HpyF3I CTNAG 2 cut(s) 961, 2028
HpySE526I ACGT 6 cut(s) 453, 707, 1210, 1317, 1527, 1701
Hsp92I GRCGYC 3 cut(s) 110, 1251, 1701
HspAI GCGC 4 cut(s) 95, 110, 1251, 2165
KasI GGCGCC 2 cut(s) 109, 1250
KpnI GGTACC 1 cut(s) 270
Kzo9I GATC 4 cut(s) 151, 646, 1424, 1609
LmnI GCTCC 6 cut(s) 394, 409, 718, 1091, 1178, 1586
Lsp1109I GCAGC 6 cut(s) 46, 101, 104, 384, 434, 684
LweI GCATC 5 cut(s) 665, 1469, 1772, 1996, 2152
MaeII ACGT 6 cut(s) 453, 707, 1210, 1317, 1527, 1701
MaeIII GTNAC 5 cut(s) 28, 221, 887, 1697, 2043
MalI GATC 4 cut(s) 153, 648, 1426, 1611
MboI GATC 4 cut(s) 151, 646, 1424, 1609
MboII GAAGA 5 cut(s) 497, 704, 1136, 1465, 1797
MhlI GDGCHC 2 cut(s) 574, 1480
MlsI TGGCCA 1 cut(s) 1952
MluNI TGGCCA 1 cut(s) 1952
Mly113I GGCGCC 2 cut(s) 110, 1251
MlyI GAGTC 1 cut(s) 557
Mox20I TGGCCA 1 cut(s) 1952
MroXI GAANNNNTTC 2 cut(s) 1037, 1668
MscI TGGCCA 1 cut(s) 1952
MseI TTAA 8 cut(s) 47, 771, 977, 1058, 1278, 1736, 2079, 2147
MslI CAYNNNNRTG 1 cut(s) 1158
Msp20I TGGCCA 1 cut(s) 1952
MspA1I CMGCKG 2 cut(s) 381, 1121
MspI CCGG 4 cut(s) 107, 203, 761, 856
MspR9I CCNGG 2 cut(s) 652, 909
Mva1269I GAATGC 1 cut(s) 1974
MvaI CCWGG 2 cut(s) 652, 909
MvnI CGCG 2 cut(s) 1594, 2167
MwoI GCNNNNNNNGC 5 cut(s) 328, 909, 1642, 1895, 1993
NarI GGCGCC 2 cut(s) 110, 1251
NcoI CCATGG 3 cut(s) 1384, 1953, 1991
NdeI CATATG 1 cut(s) 2155
NdeII GATC 4 cut(s) 151, 646, 1424, 1609
NheI GCTAGC 1 cut(s) 85
NlaIV GGNNCC 7 cut(s) 111, 268, 923, 1093, 1180, 1252, 1659
NmeAIII GCCGAG 1 cut(s) 965
NmuCI GTSAC 3 cut(s) 221, 1697, 2043
NspI RCATGY 1 cut(s) 2134
PaeI GCATGC 1 cut(s) 2134
PasI CCCWGGG 1 cut(s) 651
PceI AGGCCT 1 cut(s) 1933
PcsI WCGNNNNNNNCGW 2 cut(s) 684, 713
PctI GAATGC 1 cut(s) 1974
PdmI GAANNNNTTC 2 cut(s) 1037, 1668
PfeI GAWTC 2 cut(s) 22, 1165
PkrI GCNGC 8 cut(s) 36, 61, 91, 94, 383, 399, 449, 674
PleI GAGTC 1 cut(s) 556
PluTI GGCGCC 2 cut(s) 113, 1254
PpsI GAGTC 1 cut(s) 556
Ppu21I YACGTR 1 cut(s) 1211
Psp1406I AACGTT 1 cut(s) 453
Psp6I CCWGG 2 cut(s) 650, 907
PspEI GGTNACC 1 cut(s) 2043
PspFI CCCAGC 1 cut(s) 2038
PspGI CCWGG 2 cut(s) 650, 907
PspN4I GGNNCC 7 cut(s) 111, 268, 923, 1093, 1180, 1252, 1659
PspPI GGNCC 2 cut(s) 619, 1657
PstI CTGCAG 1 cut(s) 886
PstNI CAGNNNCTG 1 cut(s) 904
RsaI GTAC 7 cut(s) 268, 367, 862, 1037, 1376, 2033, 2110
RsaNI GTAC 7 cut(s) 267, 366, 861, 1036, 1375, 2032, 2109
RseI CAYNNNNRTG 1 cut(s) 1158
SalI GTCGAC 1 cut(s) 851
SaqAI TTAA 8 cut(s) 47, 771, 977, 1058, 1278, 1736, 2079, 2147
SatI GCNGC 8 cut(s) 35, 60, 90, 93, 382, 398, 448, 673
Sau3AI GATC 4 cut(s) 151, 646, 1424, 1609
Sau96I GGNCC 2 cut(s) 619, 1657
ScaI AGTACT 2 cut(s) 1037, 2033
SchI GAGTC 1 cut(s) 557
ScrFI CCNGG 2 cut(s) 652, 909
SduI GDGCHC 2 cut(s) 574, 1480
SfaNI GCATC 5 cut(s) 665, 1469, 1772, 1996, 2152
SfcI CTRYAG 2 cut(s) 882, 1705
SfoI GGCGCC 2 cut(s) 111, 1252
SinI GGWCC 1 cut(s) 1657
SmiMI CAYNNNNRTG 1 cut(s) 1158
SmlI CTYRAG 2 cut(s) 117, 1130
SmoI CTYRAG 2 cut(s) 117, 1130
SpeI ACTAGT 2 cut(s) 1363, 1630
SphI GCATGC 1 cut(s) 2134
SseBI AGGCCT 1 cut(s) 1933
SsiI CCGC 5 cut(s) 60, 381, 521, 1121, 1430
SspDI GGCGCC 2 cut(s) 109, 1250
StuI AGGCCT 1 cut(s) 1933
StyD4I CCNGG 2 cut(s) 650, 907
StyI CCWWGG 3 cut(s) 1384, 1953, 1991
TaaI ACNGT 5 cut(s) 238, 365, 442, 1052, 1192
TaiI ACGT 6 cut(s) 456, 710, 1213, 1320, 1530, 1704
TaqI TCGA 4 cut(s) 608, 852, 1344, 1522
TatI WGTACW 3 cut(s) 1035, 1374, 2031
TauI GCSGC 2 cut(s) 62, 384
TfiI GAWTC 2 cut(s) 22, 1165
Tru1I TTAA 8 cut(s) 47, 771, 977, 1058, 1278, 1736, 2079, 2147
Tru9I TTAA 8 cut(s) 47, 771, 977, 1058, 1278, 1736, 2079, 2147
TscAI CASTG 4 cut(s) 87, 241, 905, 2122
TseFI GTSAC 3 cut(s) 221, 1697, 2043
TseI GCWGC 6 cut(s) 34, 89, 92, 397, 447, 672
Tsp45I GTSAC 3 cut(s) 221, 1697, 2043
TspDTI ATGAA 8 cut(s) 498, 673, 1016, 1425, 1588, 1661, 1955, 2109
TspRI CASTG 4 cut(s) 87, 241, 905, 2122
VpaK11BI GGWCC 1 cut(s) 1657
XagI CCTNNNNNAGG 1 cut(s) 1133
XapI RAATTY 6 cut(s) 206, 296, 1203, 1833, 2008, 2136
XbaI TCTAGA 1 cut(s) 1606
XceI RCATGY 1 cut(s) 2134
XcmI CCANNNNNNNNNTGG 1 cut(s) 1960
XmiI GTMKAC 2 cut(s) 852, 1704
XmnI GAANNNNTTC 2 cut(s) 1037, 1668
ZraI GACGTC 1 cut(s) 1702
ZrmI AGTACT 2 cut(s) 1037, 2033
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.