Rroxscaffold_1G00060110

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000066
Physical Location & Seq
Forward (+)
82268834 .. 82275655
6822 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00060110.1

Sequence Viewer

Length: 1278 bp
ATGCCACCTCCGAGCTCCTCCACCACACTCCCCGCTCTCCGCCTCTCCAAGAAGCTCCGCAACCTCGACGAAACAAAAATTGATGACGAAAATAACATCCATCGACCAAATTTTCCAACAATCTTAAGAGTAGACTTGTTGGTGGATGTTTCCTTGAAACAAAAATTGATGACGAAAATAACATCCATCGACCAAATTTTCCAACGGGATGAGACGAAAGGTTTGGCGTTGGTGCGCCGGTCGGAGGGACGACCAAGACATTTCATTACCAAGTCTTCCTTCTACTTGTCTACAAAAAGCGAGGCTTGCAAGCACAACTTCACTCGTAAGAAATACATTTATACATATACACCTCTCAATTTGAGTACTAAATCAATTGGTGCAAGCAGGTTACTAAGTATGCAATTCATACTGGTAGCGATACTGGTTCTCTTCAAATTTGCGGCCGCGGCCGAAGAAGCAGTAGCAGCTGAAGCCGTCCTGCGTCAAGCCAAGCCTGGCTGCACTGACAGCTGTGGCAACCTCACCATTCCATATCCATTTGGAATAGGGCCTGATTGTTACATGACCAAAGACTTTTCTGTTCGTTGTAACACATCCACTAAACCCCCGACAGCAATGTGGGATGATAGTCCCATCGCTATTAACAACATATACCTGGCTGAAGGTGAGATGCAAATAGCGCAGTACGTAGCCTACGATTGCTATGACAGCCAGGGCTGGACTAATTCCAGCGAACCATTGCTCGCCGTGCCTCCTTTCACCATCTCTCATACTAGAAACAAGTTCATAGCCATTGGTTGCGATGCTTATGCACTTTTCAGAGGTTACCGTGCAGACGAAGAGAGGTACGTGACCGGATGCATGTCCATATGTGACAACCTTGACAGCGTTGAACAGTCTTGCTCCGGGGTTGGGTGTTGCCAAACAGACATCCCTAGAGGACTTAAGAACTACACTGTCAGACTGGATAGCTACTATAACCATACATACATACTGGACTTCAATCCATGCAGCTATGCCTTCATTGTGGAAGAAGGCAAGTTTACCTTTTCCCCGAATACAAGTTTTAAAGACCTCTCCAACACTGAAGAGCTTCCCACGATTCTTAATTGGGAAATTGGGGACGGGCCATGTGATGAAGCTCAAAAGAAATATGATTATGCATGCAAGGCAAATAGTAAGTGTGTCAATCGGAACATCACCGATGAGCTATCTGGTTATATTTGTCAATTGTCACTGCCAGCCGGGTTATCAAGGGAACCCCTACCTCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK

Protein Analysis

425

Amino Acids

47.62

Weight (kDa)

6.27

Isoelectric Point (pI)

48.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 168 - 228 3.3e-10 Wall-associated receptor kinase galacturonan-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21210 AT1G21230 AT1G21240 AT1G21240 AT1G21245 AT1G21250 AT1G21270 AT1G22720 AT3G25490
fragaria_vesca FvH4_2g35051 FvH4_2g35051 FvH4_2g35060 FvH4_3g10281 FvH4_3g10285 FvH4_3g10286 FvH4_3g10311 FvH4_3g10311 FvH4_3g10320 FvH4_3g10332 FvH4_3g10340 FvH4_3g10350 FvH4_3g10360 FvH4_3g10390 FvH4_3g10412 FvH4_3g10413 FvH4_3g10461 FvH4_3g10462 FvH4_3g10470 FvH4_3g10510 FvH4_3g10520 FvH4_3g10530 FvH4_3g10550 FvH4_3g10560 FvH4_3g10611 FvH4_3g11730 FvH4_6g53140 FvH4_6g53142 FvH4_7g31880 FvH4_7g31910 FvH4_7g31920
malus_domestica MD05G1269900.v1.1 MD05G1270000.v1.1 MD05G1270200.v1.1 MD05G1270400.v1.1 MD05G1270500.v1.1 MD05G1270600.v1.1 MD05G1270800.v1.1 MD05G1270900.v1.1 MD07G1183800.v1.1 MD08G1107600.v1.1 MD10G1248500.v1.1 MD10G1249200.v1.1 MD10G1249500.v1.1 MD10G1250000.v1.1 MD10G1250500.v1.1 MD10G1250900.v1.1 MD10G1251200.v1.1 MD10G1251400.v1.1 MD13G1004600.v1.1 MD15G1088400.v1.1
prunus_persica Prupe.1G442400_v2.0.a1 Prupe.1G442500_v2.0.a1 Prupe.2G223400_v2.0.a1 Prupe.4G091900_v2.0.a1 Prupe.4G093200_v2.0.a1 Prupe.4G093300_v2.0.a1 Prupe.4G093400_v2.0.a1 Prupe.4G093500_v2.0.a1 Prupe.4G094100_v2.0.a1 Prupe.4G094200_v2.0.a1 Prupe.4G094300_v2.0.a1 Prupe.6G358300_v2.0.a1
pyrus_communis pycom08g08990 pycom10g20660 pycom10g20670 pycom10g20680 pycom10g20710 pycom10g20740 pycom13g00400 pycom15g08290
rosa_chinensis RchiOBHm_Chr1g0326861 RchiOBHm_Chr1g0326871 RchiOBHm_Chr1g0331391 RchiOBHm_Chr1g0336911 RchiOBHm_Chr1g0364001 RchiOBHm_Chr2g0174541 RchiOBHm_Chr2g0175041 RchiOBHm_Chr2g0175101 RchiOBHm_Chr2g0175121 RchiOBHm_Chr3g0486331 RchiOBHm_Chr5g0015581 RchiOBHm_Chr5g0016551 RchiOBHm_Chr5g0016561 RchiOBHm_Chr5g0016581 RchiOBHm_Chr5g0016601 RchiOBHm_Chr5g0016611 RchiOBHm_Chr5g0016641 RchiOBHm_Chr5g0016661 RchiOBHm_Chr5g0016691 RchiOBHm_Chr5g0016701 RchiOBHm_Chr5g0016731 RchiOBHm_Chr5g0016741 RchiOBHm_Chr5g0016811 RchiOBHm_Chr5g0016841 RchiOBHm_Chr5g0016871 RchiOBHm_Chr5g0016881 RchiOBHm_Chr5g0016931 RchiOBHm_Chr5g0017021 RchiOBHm_Chr5g0017041 RchiOBHm_Chr5g0017071 RchiOBHm_Chr5g0017091 RchiOBHm_Chr5g0017121 RchiOBHm_Chr5g0017141 RchiOBHm_Chr5g0017291 RchiOBHm_Chr5g0017321 RchiOBHm_Chr5g0017401 RchiOBHm_Chr5g0019001 RchiOBHm_Chr5g0019071 RchiOBHm_Chr5g0028941 RchiOBHm_Chr5g0052141 RchiOBHm_Chr5g0054721 RchiOBHm_Chr5g0054801 RchiOBHm_Chr5g0054931 RchiOBHm_Chr5g0054941 RchiOBHm_Chr6g0259291 RchiOBHm_Chr6g0292991 RchiOBHm_Chr6g0306911
rosa_laevigata RLG00000009465 RLG00000009468 RLG00000010755 RLG00000011976 RLG00000014541 RLG00000014542 RLG00000015169 RLG00000022308 RLG00000027529 RLG00000029324 RLG00000030244 RLG00000030245 RLG00000032210 RLG00000032211 RLG00000032257 RLG00000032259 RLG00000032260 RLG00000032261 RLG00000032262 RLG00000032268 RLG00000032269 RLG00000032272 RLG00000032282 RLG00000032283 RLG00000032308 RLG00000032413 RLG00000033129 RLG00000034945
rosa_multiflora Rmu_co8241929.1_g000001 Rmu_co8269227.1_g000001 Rmu_co8461259.1_g000001 Rmu_co8495385.1_g000001 Rmu_sc0000028.1_g000003 Rmu_sc0000083.1_g000009 Rmu_sc0000083.1_g000010 Rmu_sc0000083.1_g000031 Rmu_sc0000083.1_g000043 Rmu_sc0000083.1_g000046 Rmu_sc0000083.1_g000047 Rmu_sc0000563.1_g000024 Rmu_sc0000842.1_g000012 Rmu_sc0001235.1_g000001 Rmu_sc0001235.1_g000005 Rmu_sc0001235.1_g000009 Rmu_sc0001235.1_g000015 Rmu_sc0001235.1_g000016 Rmu_sc0001235.1_g000017 Rmu_sc0001473.1_g000036 Rmu_sc0001607.1_g000016 Rmu_sc0001788.1_g000006 Rmu_sc0002018.1_g000008 Rmu_sc0002028.1_g000001 Rmu_sc0002680.1_g000011 Rmu_sc0002798.1_g000023 Rmu_sc0002886.1_g000023 Rmu_sc0002989.1_g000012 Rmu_sc0003064.1_g000009 Rmu_sc0003382.1_g000006 Rmu_sc0003630.1_g000002 Rmu_sc0003630.1_g000047 Rmu_sc0003630.1_g000066 Rmu_sc0003630.1_g000067 Rmu_sc0003649.1_g000010 Rmu_sc0003688.1_g000005 Rmu_sc0003920.1_g000007 Rmu_sc0004765.1_g000007 Rmu_sc0004765.1_g000008 Rmu_sc0004920.1_g000011 Rmu_sc0005223.1_g000008 Rmu_sc0005223.1_g000010 Rmu_sc0005223.1_g000015 Rmu_sc0005223.1_g000016 Rmu_sc0005319.1_g000014 Rmu_sc0005319.1_g000015 Rmu_sc0005319.1_g000018 Rmu_sc0005319.1_g000019 Rmu_sc0005319.1_g000025 Rmu_sc0005418.1_g000007 Rmu_sc0005418.1_g000012 Rmu_sc0006106.1_g000011 Rmu_sc0006914.1_g000012 Rmu_sc0007023.1_g000001 Rmu_sc0007662.1_g000001 Rmu_sc0007920.1_g000002 Rmu_sc0007920.1_g000013 Rmu_sc0008053.1_g000016 Rmu_sc0008053.1_g000017 Rmu_sc0008053.1_g000018 Rmu_sc0008053.1_g000023 Rmu_sc0008053.1_g000050 Rmu_sc0009042.1_g000007 Rmu_sc0009042.1_g000008 Rmu_sc0010642.1_g000008 Rmu_sc0011109.1_g000002 Rmu_sc0011314.1_g000006 Rmu_sc0011974.1_g000006 Rmu_sc0012179.1_g000009 Rmu_sc0015169.1_g000001 Rmu_sc0016368.1_g000002 Rmu_sc0022659.1_g000001 Rmu_sc0027371.1_g000001 Rmu_sc0028213.1_g000001 Rmu_sc0034526.1_g000001 Rmu_sc0039366.1_g000002 Rmu_ssc0000050.1_g000084 Rmu_ssc0000050.1_g000088 Rmu_ssc0000167.1_g000017
rosa_roxburghii Rroxscaffold_1G00011340 Rroxscaffold_1G00025710 Rroxscaffold_1G00025870 Rroxscaffold_1G00026070 Rroxscaffold_1G00026170 Rroxscaffold_1G00026200 Rroxscaffold_1G00026270 Rroxscaffold_1G00050950 Rroxscaffold_1G00058900 Rroxscaffold_1G00060040 Rroxscaffold_1G00060060 Rroxscaffold_1G00060070 Rroxscaffold_1G00060080 Rroxscaffold_1G00060110 Rroxscaffold_1G00060120 Rroxscaffold_1G00060140 Rroxscaffold_1G00060170 Rroxscaffold_1G00060210 Rroxscaffold_1G00060220 Rroxscaffold_1G00060240 Rroxscaffold_1G00060250 Rroxscaffold_1G00060260 Rroxscaffold_1G00060300 Rroxscaffold_1G00060320 Rroxscaffold_1G00060330 Rroxscaffold_1G00060900 Rroxscaffold_1G00060920 Rroxscaffold_1G00073980 Rroxscaffold_2G00077560 Rroxscaffold_2G00077580 Rroxscaffold_2G00146990 Rroxscaffold_3G00236200 Rroxscaffold_3G00236210 Rroxscaffold_3G00236220 Rroxscaffold_4G00292760 Rroxscaffold_4G00315200 Rroxscaffold_4G00315240 Rroxscaffold_4G00326240 Rroxscaffold_4G00328980 Rroxscaffold_5G00341890 Rroxscaffold_7G00161350 Rroxscaffold_7G00174490 Rroxscaffold_7G00206550
rosa_rugosa Rorug01G0093000 Rorug01G0093100 Rorug01G0128800 Rorug01G0312000 Rorug01G0312000 Rorug02G0581100 Rorug03G0224600 Rorug05G0028500 Rorug05G0032700 Rorug05G0032800 Rorug05G0032900 Rorug05G0032900 Rorug05G0033000 Rorug05G0033100 Rorug05G0033300 Rorug05G0033400 Rorug05G0033900 Rorug05G0034900 Rorug05G0035000 Rorug05G0046900 Rorug05G0047500 Rorug05G0047500 Rorug05G0111600 Rorug05G0287900 Rorug05G0288700 Rorug05G0586900 Rorug06G0231700 Rorug06G0354300 Rorug06G0354400
rosa_samantha Rh1DG122200 Rh2DG686800 Rh5BG136600 Rh6AG344500 Rh6BG351900 Rh6CG359000 Rh6CG359100 Rh6CG387600 Rh6CG480500 Rh6DG344600 Rh6DG467400
rosa_wichuraiana Rw0G010250 Rw0G014820 Rw0G021400 Rw0G023100 Rw1G009450 Rw1G012100 Rw1G012180 Rw1G028360 Rw2G054260 Rw3G024370 Rw3G024420 Rw3G024470 Rw5G010850 Rw5G011010 Rw5G011020 Rw5G011050 Rw5G011080 Rw5G011120 Rw5G012300 Rw5G018470 Rw5G033830 Rw6G008930 Rw6G030070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 378
AccBSI CCGCTC 1 cut(s) 35
AccI GTMKAC 2 cut(s) 132, 290
AccII CGCG 1 cut(s) 449
AciI CCGC 6 cut(s) 33, 40, 58, 443, 447, 449
AcoI YGGCCR 2 cut(s) 444, 450
AcsI RAATTY 3 cut(s) 109, 195, 437
AcuI CTGAAG 3 cut(s) 492, 684, 1110
AfaI GTAC 3 cut(s) 367, 689, 851
AfiI CCNNNNNNNGG 2 cut(s) 244, 915
AflII CTTAAG 2 cut(s) 124, 947
AgsI TTSAA 4 cut(s) 157, 436, 896, 1006
AjnI CCWGG 3 cut(s) 496, 657, 714
AjuI GAANNNNNNNTTGG 2 cut(s) 263, 295
AluBI AGCT 9 cut(s) 15, 55, 470, 513, 975, 1017, 1096, 1145, 1213
AluI AGCT 9 cut(s) 15, 55, 470, 513, 975, 1017, 1096, 1145, 1213
Alw21I GWGCWC 1 cut(s) 17
Alw26I GTCTC 1 cut(s) 206
AoxI GGCC 4 cut(s) 444, 450, 551, 1130
ApeKI GCWGC 3 cut(s) 467, 501, 1014
ApoI RAATTY 3 cut(s) 109, 195, 437
ArsI GACNNNNNNTTYG 2 cut(s) 205, 237
Asp700I GAANNNNTTC 1 cut(s) 1095
AspLEI GCGC 2 cut(s) 237, 685
AspS9I GGNCC 2 cut(s) 551, 1130
AsuC2I CCSGG 2 cut(s) 910, 1249
AsuHPI GGTGA 4 cut(s) 517, 680, 754, 1195
BanII GRGCYC 1 cut(s) 17
BbsI GAAGAC 1 cut(s) 267
Bbv12I GWGCWC 1 cut(s) 17
BbvI GCAGC 3 cut(s) 479, 488, 1026
BccI CCATC 4 cut(s) 108, 194, 644, 773
BceAI ACGGC 2 cut(s) 461, 734
BcgI CGANNNNNNTGC 2 cut(s) 794, 828
BciT130I CCWGG 3 cut(s) 498, 659, 716
BcnI CCSGG 2 cut(s) 910, 1249
BcoDI GTCTC 1 cut(s) 206
BfaI CTAG 2 cut(s) 777, 939
BfrI CTTAAG 2 cut(s) 124, 947
BfuAI ACCTGC 1 cut(s) 378
BisI GCNGC 6 cut(s) 444, 447, 450, 468, 502, 1015
BlsI GCNGC 6 cut(s) 445, 448, 451, 469, 503, 1016
BmcAI AGTACT 1 cut(s) 367
Bme1390I CCNGG 5 cut(s) 498, 659, 716, 910, 1249
BmgT120I GGNCC 2 cut(s) 551, 1130
BmiI GGNNCC 1 cut(s) 1263
BmrFI CCNGG 5 cut(s) 498, 659, 716, 910, 1249
BmsI GCATC 3 cut(s) 663, 796, 851
BpiI GAAGAC 1 cut(s) 267
BpuMI CCSGG 2 cut(s) 910, 1249
BsaAI YACGTR 2 cut(s) 691, 853
BsaJI CCNNGG 3 cut(s) 447, 715, 909
BsaWI WCCGGW 1 cut(s) 857
Bsc4I CCNNNNNNNGG 2 cut(s) 244, 915
Bse118I RCCGGY 1 cut(s) 237
Bse1I ACTGG 4 cut(s) 417, 429, 972, 1002
Bse3DI GCAATG 2 cut(s) 624, 740
BseBI CCWGG 3 cut(s) 498, 659, 716
BseDI CCNNGG 3 cut(s) 447, 715, 909
BseGI GGATG 8 cut(s) 96, 151, 182, 214, 596, 631, 866, 933
BseLI CCNNNNNNNGG 2 cut(s) 244, 915
BseMI GCAATG 2 cut(s) 624, 740
BseNI ACTGG 4 cut(s) 417, 429, 972, 1002
BseRI GAGGAG 1 cut(s) 7
BseX3I CGGCCG 2 cut(s) 444, 450
BseXI GCAGC 3 cut(s) 479, 488, 1026
BsgI GTGCAG 2 cut(s) 487, 855
Bsh1236I CGCG 1 cut(s) 449
Bsh1285I CGRYCG 3 cut(s) 242, 447, 453
BshFI GGCC 4 cut(s) 446, 452, 553, 1132
BsiEI CGRYCG 3 cut(s) 242, 447, 453
BsiHKAI GWGCWC 1 cut(s) 17
BsiSI CCGG 4 cut(s) 238, 858, 909, 1248
BslFI GGGAC 3 cut(s) 261, 618, 1139
BslI CCNNNNNNNGG 2 cut(s) 244, 915
BsmAI GTCTC 1 cut(s) 206
BsmBI CGTCTC 1 cut(s) 206
BsmFI GGGAC 3 cut(s) 261, 618, 1139
BsnI GGCC 4 cut(s) 446, 452, 553, 1132
Bsp1286I GDGCHC 1 cut(s) 17
BspACI CCGC 6 cut(s) 33, 40, 58, 443, 447, 449
BspANI GGCC 4 cut(s) 446, 452, 553, 1132
BspFNI CGCG 1 cut(s) 449
BspLI GGNNCC 1 cut(s) 1263
BspMI ACCTGC 1 cut(s) 378
BspQI GCTCTTC 1 cut(s) 1086
BspTI CTTAAG 2 cut(s) 124, 947
BsrBI CCGCTC 1 cut(s) 35
BsrDI GCAATG 2 cut(s) 624, 740
BsrFI RCCGGY 1 cut(s) 237
BsrI ACTGG 4 cut(s) 417, 429, 972, 1002
BssAI RCCGGY 1 cut(s) 237
BssECI CCNNGG 3 cut(s) 447, 715, 909
Bst2UI CCWGG 3 cut(s) 498, 659, 716
Bst4CI ACNGT 3 cut(s) 833, 900, 961
Bst6I CTCTTC 3 cut(s) 437, 837, 1086
BstAFI CTTAAG 2 cut(s) 124, 947
BstBAI YACGTR 2 cut(s) 691, 853
BstC8I GCNNGC 6 cut(s) 307, 311, 385, 747, 1168, 1245
BstDEI CTNAG 1 cut(s) 395
BstDSI CCRYGG 1 cut(s) 447
BstEII GGTNACC 1 cut(s) 827
BstF5I GGATG 8 cut(s) 96, 151, 182, 214, 596, 631, 866, 933
BstFNI CGCG 1 cut(s) 449
BstHHI GCGC 2 cut(s) 237, 685
BstMAI GTCTC 1 cut(s) 206
BstMCI CGRYCG 3 cut(s) 242, 447, 453
BstNI CCWGG 3 cut(s) 498, 659, 716
BstNSI RCATGY 2 cut(s) 868, 1170
BstPI GGTNACC 1 cut(s) 827
BstSCI CCNGG 5 cut(s) 496, 657, 714, 908, 1247
BstSNI TACGTA 1 cut(s) 691
BstUI CGCG 1 cut(s) 449
BstV1I GCAGC 3 cut(s) 479, 488, 1026
BstV2I GAAGAC 1 cut(s) 267
BstZI CGGCCG 2 cut(s) 444, 450
BsuRI GGCC 4 cut(s) 446, 452, 553, 1132
BtgI CCRYGG 1 cut(s) 447
BtgZI GCGATG 2 cut(s) 622, 819
BtsCI GGATG 8 cut(s) 96, 151, 182, 214, 596, 631, 866, 933
BtsI GCAGTG 1 cut(s) 1238
BtsIMutI CAGTG 4 cut(s) 504, 957, 1086, 1238
BveI ACCTGC 1 cut(s) 378
Cac8I GCNNGC 6 cut(s) 307, 311, 385, 747, 1168, 1245
CciNI GCGGCCGC 1 cut(s) 444
CfoI GCGC 2 cut(s) 237, 685
Cfr10I RCCGGY 1 cut(s) 237
Cfr13I GGNCC 2 cut(s) 551, 1130
Cfr42I CCGCGG 1 cut(s) 450
CseI GACGC 1 cut(s) 473
Csp6I GTAC 3 cut(s) 366, 688, 850
CviAII CATG 5 cut(s) 565, 865, 1011, 1134, 1167
CviQI GTAC 3 cut(s) 366, 688, 850
DdeI CTNAG 1 cut(s) 395
DraI TTTAAA 1 cut(s) 1072
EaeI YGGCCR 2 cut(s) 444, 450
EagI CGGCCG 2 cut(s) 444, 450
Eam1104I CTCTTC 3 cut(s) 437, 837, 1086
EarI CTCTTC 3 cut(s) 437, 837, 1086
EciI GGCGGA 1 cut(s) 29
Ecl136II GAGCTC 1 cut(s) 15
EclXI CGGCCG 2 cut(s) 444, 450
Eco105I TACGTA 1 cut(s) 691
Eco24I GRGCYC 1 cut(s) 17
Eco52I CGGCCG 2 cut(s) 444, 450
Eco53kI GAGCTC 1 cut(s) 15
Eco57I CTGAAG 3 cut(s) 492, 684, 1110
Eco91I GGTNACC 1 cut(s) 827
EcoICRI GAGCTC 1 cut(s) 15
EcoO109I RGGNCCY 1 cut(s) 551
EcoO65I GGTNACC 1 cut(s) 827
EcoRII CCWGG 3 cut(s) 496, 657, 714
EcoT22I ATGCAT 2 cut(s) 866, 1168
EcoT38I GRGCYC 1 cut(s) 17
Esp3I CGTCTC 1 cut(s) 206
FaeI CATG 5 cut(s) 568, 868, 1014, 1137, 1170
FalI AAGNNNNNCTT 8 cut(s) 263, 295, 289, 321, 302, 334, 1034, 1066
FaqI GGGAC 3 cut(s) 261, 618, 1139
FatI CATG 5 cut(s) 564, 864, 1010, 1133, 1166
FauI CCCGC 1 cut(s) 40
FauNDI CATATG 1 cut(s) 872
FblI GTMKAC 2 cut(s) 132, 290
Fnu4HI GCNGC 6 cut(s) 444, 447, 450, 468, 502, 1015
FokI GGATG 8 cut(s) 83, 158, 169, 221, 583, 638, 873, 920
FriOI GRGCYC 1 cut(s) 17
Fsp4HI GCNGC 6 cut(s) 444, 447, 450, 468, 502, 1015
FspBI CTAG 2 cut(s) 777, 939
GlaI GCGC 2 cut(s) 236, 684
GluI GCNGC 6 cut(s) 444, 447, 450, 468, 502, 1015
HaeIII GGCC 4 cut(s) 446, 452, 553, 1132
HapII CCGG 4 cut(s) 238, 858, 909, 1248
HgaI GACGC 1 cut(s) 473
HhaI GCGC 2 cut(s) 237, 685
Hin1II CATG 5 cut(s) 568, 868, 1014, 1137, 1170
Hin6I GCGC 2 cut(s) 235, 683
HinP1I GCGC 2 cut(s) 235, 683
HinfI GANTC 1 cut(s) 1105
HpaII CCGG 4 cut(s) 238, 858, 909, 1248
HphI GGTGA 4 cut(s) 517, 680, 754, 1195
Hpy166II GTNNAC 3 cut(s) 133, 291, 1047
Hpy188I TCNGA 5 cut(s) 12, 244, 824, 965, 1197
Hpy8I GTNNAC 3 cut(s) 133, 291, 1047
Hpy99I CGWCG 1 cut(s) 71
HpyAV CCTTC 4 cut(s) 289, 659, 1031, 1033
HpyCH4III ACNGT 3 cut(s) 833, 900, 961
HpyCH4IV ACGT 2 cut(s) 690, 852
HpyF3I CTNAG 1 cut(s) 395
HpySE526I ACGT 2 cut(s) 690, 852
Hsp92II CATG 5 cut(s) 568, 868, 1014, 1137, 1170
HspAI GCGC 2 cut(s) 235, 683
KspI CCGCGG 1 cut(s) 450
LguI GCTCTTC 1 cut(s) 1086
LmnI GCTCC 3 cut(s) 20, 60, 911
Lsp1109I GCAGC 3 cut(s) 479, 488, 1026
LweI GCATC 3 cut(s) 663, 796, 851
MaeI CTAG 2 cut(s) 777, 939
MaeII ACGT 2 cut(s) 690, 852
MaeIII GTNAC 7 cut(s) 390, 560, 590, 827, 853, 875, 1236
MbiI CCGCTC 1 cut(s) 35
MboII GAAGA 6 cut(s) 267, 424, 467, 854, 1046, 1103
MfeI CAATTG 2 cut(s) 375, 1232
MhlI GDGCHC 1 cut(s) 17
MmeI TCCRAC 4 cut(s) 140, 222, 226, 1107
Mph1103I ATGCAT 2 cut(s) 866, 1168
MroXI GAANNNNTTC 1 cut(s) 1095
MseI TTAA 5 cut(s) 125, 645, 948, 1071, 1110
MspA1I CMGCKG 3 cut(s) 449, 470, 513
MspCI CTTAAG 2 cut(s) 124, 947
MspI CCGG 4 cut(s) 238, 858, 909, 1248
MspR9I CCNGG 5 cut(s) 498, 659, 716, 910, 1249
MunI CAATTG 2 cut(s) 375, 1232
MvaI CCWGG 3 cut(s) 498, 659, 716
MvnI CGCG 1 cut(s) 449
NciI CCSGG 2 cut(s) 910, 1249
NdeI CATATG 1 cut(s) 872
NlaIII CATG 5 cut(s) 568, 868, 1014, 1137, 1170
NlaIV GGNNCC 1 cut(s) 1263
NmuCI GTSAC 3 cut(s) 853, 875, 1236
NotI GCGGCCGC 1 cut(s) 444
NsiI ATGCAT 2 cut(s) 866, 1168
NspI RCATGY 2 cut(s) 868, 1170
PaeI GCATGC 1 cut(s) 1170
PciSI GCTCTTC 1 cut(s) 1086
PcsI WCGNNNNNNNCGW 1 cut(s) 696
PdmI GAANNNNTTC 1 cut(s) 1095
PfeI GAWTC 1 cut(s) 1105
PkrI GCNGC 6 cut(s) 445, 448, 451, 469, 503, 1016
Ppu21I YACGTR 2 cut(s) 691, 853
Psp124BI GAGCTC 1 cut(s) 17
Psp6I CCWGG 3 cut(s) 496, 657, 714
PspEI GGTNACC 1 cut(s) 827
PspGI CCWGG 3 cut(s) 496, 657, 714
PspN4I GGNNCC 1 cut(s) 1263
PspPI GGNCC 2 cut(s) 551, 1130
PvuII CAGCTG 2 cut(s) 470, 513
RsaI GTAC 3 cut(s) 367, 689, 851
RsaNI GTAC 3 cut(s) 366, 688, 850
SacI GAGCTC 1 cut(s) 17
SacII CCGCGG 1 cut(s) 450
SapI GCTCTTC 1 cut(s) 1086
SaqAI TTAA 5 cut(s) 125, 645, 948, 1071, 1110
SatI GCNGC 6 cut(s) 444, 447, 450, 468, 502, 1015
Sau96I GGNCC 2 cut(s) 551, 1130
ScaI AGTACT 1 cut(s) 367
ScrFI CCNGG 5 cut(s) 498, 659, 716, 910, 1249
SduI GDGCHC 1 cut(s) 17
SfaNI GCATC 3 cut(s) 663, 796, 851
Sfr303I CCGCGG 1 cut(s) 450
SgrBI CCGCGG 1 cut(s) 450
SmlI CTYRAG 2 cut(s) 124, 947
SmoI CTYRAG 2 cut(s) 124, 947
SnaBI TACGTA 1 cut(s) 691
SphI GCATGC 1 cut(s) 1170
SsiI CCGC 6 cut(s) 33, 40, 58, 443, 447, 449
SspMI CTAG 2 cut(s) 777, 939
SstI GAGCTC 1 cut(s) 17
StyD4I CCNGG 5 cut(s) 496, 657, 714, 908, 1247
TaaI ACNGT 3 cut(s) 833, 900, 961
TaiI ACGT 2 cut(s) 693, 855
TaqI TCGA 3 cut(s) 66, 103, 189
TatI WGTACW 1 cut(s) 365
TauI GCSGC 3 cut(s) 446, 449, 452
TfiI GAWTC 1 cut(s) 1105
Tru1I TTAA 5 cut(s) 125, 645, 948, 1071, 1110
Tru9I TTAA 5 cut(s) 125, 645, 948, 1071, 1110
TscAI CASTG 4 cut(s) 511, 964, 1093, 1245
TseFI GTSAC 3 cut(s) 853, 875, 1236
TseI GCWGC 3 cut(s) 467, 501, 1014
Tsp45I GTSAC 3 cut(s) 853, 875, 1236
TspDTI ATGAA 5 cut(s) 253, 397, 778, 1015, 1155
TspRI CASTG 4 cut(s) 511, 964, 1093, 1245
Vha464I CTTAAG 2 cut(s) 124, 947
XapI RAATTY 3 cut(s) 109, 195, 437
XceI RCATGY 2 cut(s) 868, 1170
XmiI GTMKAC 2 cut(s) 132, 290
XmnI GAANNNNTTC 1 cut(s) 1095
XspI CTAG 2 cut(s) 777, 939
ZrmI AGTACT 1 cut(s) 367
Zsp2I ATGCAT 2 cut(s) 866, 1168
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.