Prupe.4G093500_v2.0.a1

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0000066
Physical Location & Seq
Reverse (-)
4709207 .. 4711902
2696 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G093500.1

Sequence Viewer

Length: 2283 bp
ATGATTATAATTATGCAACTCTCTCTGGTTGTGATACTAGGGGCTGCAATATTTACAACAATAGCAGCAGCTCCAGCCCTGCCTCAAGCCCTGCCTGGGTGCAGAGACAAATGTGGTGATCTAACAATTCCTTTCCCATTTGGCATAGGTGATGGCTGCTACCTTCGACCCGAATTCAACCTCACATGCGACCAATCCACCACGCCGCCATCGGCGAACTTGACGGGCCACACAATCCGCATTTCTGACTTTTACCTTGCTGAGGCAGAGTTGCAAGTAATGACCTTCACATCCAGAGATTGTTATGACACCAAAGGTCTCAGAGCCTACAGGAACACCCCCATACTCTGGCTGCCTCCTCCTTATACCATCTCCAACACCAAGAACAAGTTTGTCGCCATTGGTTGCGACACATACGCGCTTTTCCAAGGATACAGAGGCGAAGAGAGGTTCATAACCGGGTGCATGTCCTTATGTGACAGCCTAGGCAGCGTCGAATACCAGGACTCTTATTGCTCCGGCATTGGGTGCTGTCAAACTTCCATCCCCAGTGGACTGAAAAACTGTACTGTCCAATTGAGTAGCTATTATAATCATACTTTCATAATGGACTTCAACCCCTGCAGCTATGCTTTCATTGTGGAACAGGGCCAGTTCAAATTCAACAGTACAAGTTTTCAACAACTCAACAGCATAAGTCAAATTCCAATGGTTATAAATTGGGAAATTGGGGATGAGACATGCGATATTGCAGCTCAAAAGAGAGAAGATTTTGTATGCAAGGCAGATTATAGCGAGTGTGTCAACAGGAACACTACTATCAATGGGTCTGGTTACATTTGCCAGTGCTTGCCAGGTTACCAAGGGAATCCATACCTCCCACATGGTTGCCAAGATTTTGATGAGTGCAAGGCTTTGAACCCCTGCAGTATTGGATCCTGTGAGAACTTAGATGGAAATTATTCTTGTAGATGTCCCAAAGGGTACAAAAATGACGGCACGAATAAACAGAGTTGCATCAAAGATGACCCCAACAATCTCCTGCTGATTATTTCATTGGGTGTAAGTGGAGGTTTCTTGGTTTTACTGGTTGGAATTTCACTGATATATTGGCGAATGCAGAGAAGAAAGTTCACCAAGCTCAAAGAACAATACTTTATACAAAATGGTGGCTTATTGTTACAAGAACAACTTGCTAGTCAAGTAGGTGCTGTGGAGACAACGAAAATCTTTACTGCAGAAGAACTTGAGAGAGCCACAAACAATTACCATGAAAGTAGAATTCTCGGTGAAGGAGGATACGGAATAGTTTACAAAGGAATATTGCCGGATAGCAACCGAGTAGTTGCCATAAAGAAGTCCAAAATTGGTGTCCCAACACAGAAGGAGCAGTTTGTCAATGAGTTGATTGTTCTTTCTCAAATCAACCATAGAAATGTGGTGAGGCTATTCGGTTGCTGTTTAGAGACATCAGTGCCTTTACTAGTTTACGAGTTCATCGCGAATGGCACTCTTTTCGAGCACATCCATCATATAAATGGCAGAGGATCATCTTTTTCATGGGAATTACGATTGAAGATAGCAGCAGAAACTGCAGGAGCATTAGCATACTTACACTCCTCTGCTCTCATGCAGATCATACACCGAGATGTGAAGGCAACAAATATACTATTAGATGATAATTACATGGCAAAGGTGTCAGACTTCGGAGCTTCACGACTGATCCCTCTAGATCAAAATCAACTAACAACATTAGTGCAAGGGACATTTGGATACTTAGACCCTGAATACTTCCTTACAAATCAACTAACAGAAAAGAGTGATGTCTATAGTTTTGGAGTAGTCCTTATGGAGCTCCTAACAAGCAAAGTGGCACTTTCTTTTGCCAGGCCCGAGGAAGAGAGAAACCTAGCAAGTTTCTTCGTTTGTTCAATGGATGAAGGTCGCTTGTATCAAATTCTTGATGATGACATAGCCAACGAGAGAAATATTGAGACACTACAAAACGTGGCCGATCTCGCAAAAAGATGTGTAAGGTTAAAAGGGGAGGATAGGCCTAGCATGAAAGAAGTAGCAATGGAGTTAGAGGGAATGAGAATTGCAGCAAAACATCCATGGGGAAAAGATGATATCTGTGCAGAAGAAACTGAGTACTTTCTCGGCTCACCTAATTCAGACACTTATCTTTTGAATGTTAGAGCTGATTGTGGTCCTAGTGGTACCAGTAGTGGATATGACAGCATGCAAAACCAACTGTTGATGCCAAATGATGATGGCCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK hEGF

Protein Analysis

761

Amino Acids

84.59

Weight (kDa)

5.11

Isoelectric Point (pI)

34.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21210 AT1G21230 AT1G21240 AT1G21240 AT1G21245 AT1G21250 AT1G21270 AT1G22720 AT3G25490
fragaria_vesca FvH4_2g35051 FvH4_2g35051 FvH4_2g35060 FvH4_3g10281 FvH4_3g10285 FvH4_3g10286 FvH4_3g10311 FvH4_3g10311 FvH4_3g10320 FvH4_3g10332 FvH4_3g10340 FvH4_3g10350 FvH4_3g10360 FvH4_3g10390 FvH4_3g10412 FvH4_3g10413 FvH4_3g10461 FvH4_3g10462 FvH4_3g10470 FvH4_3g10510 FvH4_3g10520 FvH4_3g10530 FvH4_3g10550 FvH4_3g10560 FvH4_3g10611 FvH4_3g11730 FvH4_6g53140 FvH4_6g53142 FvH4_7g31880 FvH4_7g31910 FvH4_7g31920
malus_domestica MD05G1269900.v1.1 MD05G1270000.v1.1 MD05G1270200.v1.1 MD05G1270400.v1.1 MD05G1270500.v1.1 MD05G1270600.v1.1 MD05G1270800.v1.1 MD05G1270900.v1.1 MD07G1183800.v1.1 MD08G1107600.v1.1 MD10G1248500.v1.1 MD10G1249200.v1.1 MD10G1249500.v1.1 MD10G1250000.v1.1 MD10G1250500.v1.1 MD10G1250900.v1.1 MD10G1251200.v1.1 MD10G1251400.v1.1 MD13G1004600.v1.1 MD15G1088400.v1.1
prunus_persica Prupe.1G442400_v2.0.a1 Prupe.1G442500_v2.0.a1 Prupe.2G223400_v2.0.a1 Prupe.4G091900_v2.0.a1 Prupe.4G093200_v2.0.a1 Prupe.4G093300_v2.0.a1 Prupe.4G093400_v2.0.a1 Prupe.4G093500_v2.0.a1 Prupe.4G094100_v2.0.a1 Prupe.4G094200_v2.0.a1 Prupe.4G094300_v2.0.a1 Prupe.6G358300_v2.0.a1
pyrus_communis pycom08g08990 pycom10g20660 pycom10g20670 pycom10g20680 pycom10g20710 pycom10g20740 pycom13g00400 pycom15g08290
rosa_chinensis RchiOBHm_Chr1g0326861 RchiOBHm_Chr1g0326871 RchiOBHm_Chr1g0331391 RchiOBHm_Chr1g0336911 RchiOBHm_Chr1g0364001 RchiOBHm_Chr2g0174541 RchiOBHm_Chr2g0175041 RchiOBHm_Chr2g0175101 RchiOBHm_Chr2g0175121 RchiOBHm_Chr3g0486331 RchiOBHm_Chr5g0015581 RchiOBHm_Chr5g0016551 RchiOBHm_Chr5g0016561 RchiOBHm_Chr5g0016581 RchiOBHm_Chr5g0016601 RchiOBHm_Chr5g0016611 RchiOBHm_Chr5g0016641 RchiOBHm_Chr5g0016661 RchiOBHm_Chr5g0016691 RchiOBHm_Chr5g0016701 RchiOBHm_Chr5g0016731 RchiOBHm_Chr5g0016741 RchiOBHm_Chr5g0016811 RchiOBHm_Chr5g0016841 RchiOBHm_Chr5g0016871 RchiOBHm_Chr5g0016881 RchiOBHm_Chr5g0016931 RchiOBHm_Chr5g0017021 RchiOBHm_Chr5g0017041 RchiOBHm_Chr5g0017071 RchiOBHm_Chr5g0017091 RchiOBHm_Chr5g0017121 RchiOBHm_Chr5g0017141 RchiOBHm_Chr5g0017291 RchiOBHm_Chr5g0017321 RchiOBHm_Chr5g0017401 RchiOBHm_Chr5g0019001 RchiOBHm_Chr5g0019071 RchiOBHm_Chr5g0028941 RchiOBHm_Chr5g0052141 RchiOBHm_Chr5g0054721 RchiOBHm_Chr5g0054801 RchiOBHm_Chr5g0054931 RchiOBHm_Chr5g0054941 RchiOBHm_Chr6g0259291 RchiOBHm_Chr6g0292991 RchiOBHm_Chr6g0306911
rosa_laevigata RLG00000009465 RLG00000009468 RLG00000010755 RLG00000011976 RLG00000014541 RLG00000014542 RLG00000015169 RLG00000022308 RLG00000027529 RLG00000029324 RLG00000030244 RLG00000030245 RLG00000032210 RLG00000032211 RLG00000032257 RLG00000032259 RLG00000032260 RLG00000032261 RLG00000032262 RLG00000032268 RLG00000032269 RLG00000032272 RLG00000032282 RLG00000032283 RLG00000032308 RLG00000032413 RLG00000033129 RLG00000034945
rosa_multiflora Rmu_co8241929.1_g000001 Rmu_co8269227.1_g000001 Rmu_co8461259.1_g000001 Rmu_co8495385.1_g000001 Rmu_sc0000028.1_g000003 Rmu_sc0000083.1_g000009 Rmu_sc0000083.1_g000010 Rmu_sc0000083.1_g000031 Rmu_sc0000083.1_g000043 Rmu_sc0000083.1_g000046 Rmu_sc0000083.1_g000047 Rmu_sc0000563.1_g000024 Rmu_sc0000842.1_g000012 Rmu_sc0001235.1_g000001 Rmu_sc0001235.1_g000005 Rmu_sc0001235.1_g000009 Rmu_sc0001235.1_g000015 Rmu_sc0001235.1_g000016 Rmu_sc0001235.1_g000017 Rmu_sc0001473.1_g000036 Rmu_sc0001607.1_g000016 Rmu_sc0001788.1_g000006 Rmu_sc0002018.1_g000008 Rmu_sc0002028.1_g000001 Rmu_sc0002680.1_g000011 Rmu_sc0002798.1_g000023 Rmu_sc0002886.1_g000023 Rmu_sc0002989.1_g000012 Rmu_sc0003064.1_g000009 Rmu_sc0003382.1_g000006 Rmu_sc0003630.1_g000002 Rmu_sc0003630.1_g000047 Rmu_sc0003630.1_g000066 Rmu_sc0003630.1_g000067 Rmu_sc0003649.1_g000010 Rmu_sc0003688.1_g000005 Rmu_sc0003920.1_g000007 Rmu_sc0004765.1_g000007 Rmu_sc0004765.1_g000008 Rmu_sc0004920.1_g000011 Rmu_sc0005223.1_g000008 Rmu_sc0005223.1_g000010 Rmu_sc0005223.1_g000015 Rmu_sc0005223.1_g000016 Rmu_sc0005319.1_g000014 Rmu_sc0005319.1_g000015 Rmu_sc0005319.1_g000018 Rmu_sc0005319.1_g000019 Rmu_sc0005319.1_g000025 Rmu_sc0005418.1_g000007 Rmu_sc0005418.1_g000012 Rmu_sc0006106.1_g000011 Rmu_sc0006914.1_g000012 Rmu_sc0007023.1_g000001 Rmu_sc0007662.1_g000001 Rmu_sc0007920.1_g000002 Rmu_sc0007920.1_g000013 Rmu_sc0008053.1_g000016 Rmu_sc0008053.1_g000017 Rmu_sc0008053.1_g000018 Rmu_sc0008053.1_g000023 Rmu_sc0008053.1_g000050 Rmu_sc0009042.1_g000007 Rmu_sc0009042.1_g000008 Rmu_sc0010642.1_g000008 Rmu_sc0011109.1_g000002 Rmu_sc0011314.1_g000006 Rmu_sc0011974.1_g000006 Rmu_sc0012179.1_g000009 Rmu_sc0015169.1_g000001 Rmu_sc0016368.1_g000002 Rmu_sc0022659.1_g000001 Rmu_sc0027371.1_g000001 Rmu_sc0028213.1_g000001 Rmu_sc0034526.1_g000001 Rmu_sc0039366.1_g000002 Rmu_ssc0000050.1_g000084 Rmu_ssc0000050.1_g000088 Rmu_ssc0000167.1_g000017
rosa_roxburghii Rroxscaffold_1G00011340 Rroxscaffold_1G00025710 Rroxscaffold_1G00025870 Rroxscaffold_1G00026070 Rroxscaffold_1G00026170 Rroxscaffold_1G00026200 Rroxscaffold_1G00026270 Rroxscaffold_1G00050950 Rroxscaffold_1G00058900 Rroxscaffold_1G00060040 Rroxscaffold_1G00060060 Rroxscaffold_1G00060070 Rroxscaffold_1G00060080 Rroxscaffold_1G00060110 Rroxscaffold_1G00060120 Rroxscaffold_1G00060140 Rroxscaffold_1G00060170 Rroxscaffold_1G00060210 Rroxscaffold_1G00060220 Rroxscaffold_1G00060240 Rroxscaffold_1G00060250 Rroxscaffold_1G00060260 Rroxscaffold_1G00060300 Rroxscaffold_1G00060320 Rroxscaffold_1G00060330 Rroxscaffold_1G00060900 Rroxscaffold_1G00060920 Rroxscaffold_1G00073980 Rroxscaffold_2G00077560 Rroxscaffold_2G00077580 Rroxscaffold_2G00146990 Rroxscaffold_3G00236200 Rroxscaffold_3G00236210 Rroxscaffold_3G00236220 Rroxscaffold_4G00292760 Rroxscaffold_4G00315200 Rroxscaffold_4G00315240 Rroxscaffold_4G00326240 Rroxscaffold_4G00328980 Rroxscaffold_5G00341890 Rroxscaffold_7G00161350 Rroxscaffold_7G00174490 Rroxscaffold_7G00206550
rosa_rugosa Rorug01G0093000 Rorug01G0093100 Rorug01G0128800 Rorug01G0312000 Rorug01G0312000 Rorug02G0581100 Rorug03G0224600 Rorug05G0028500 Rorug05G0032700 Rorug05G0032800 Rorug05G0032900 Rorug05G0032900 Rorug05G0033000 Rorug05G0033100 Rorug05G0033300 Rorug05G0033400 Rorug05G0033900 Rorug05G0034900 Rorug05G0035000 Rorug05G0046900 Rorug05G0047500 Rorug05G0047500 Rorug05G0111600 Rorug05G0287900 Rorug05G0288700 Rorug05G0586900 Rorug06G0231700 Rorug06G0354300 Rorug06G0354400
rosa_samantha Rh1DG122200 Rh2DG686800 Rh5BG136600 Rh6AG344500 Rh6BG351900 Rh6CG359000 Rh6CG359100 Rh6CG387600 Rh6CG480500 Rh6DG344600 Rh6DG467400
rosa_wichuraiana Rw0G010250 Rw0G014820 Rw0G021400 Rw0G023100 Rw1G009450 Rw1G012100 Rw1G012180 Rw1G028360 Rw2G054260 Rw3G024370 Rw3G024420 Rw3G024470 Rw5G010850 Rw5G011010 Rw5G011020 Rw5G011050 Rw5G011080 Rw5G011120 Rw5G012300 Rw5G018470 Rw5G033830 Rw6G008930 Rw6G030070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 8, 591, 716
Acc65I GGTACC 1 cut(s) 2219
AccB1I GGYRCC 1 cut(s) 2219
AccB7I CCANNNNNTGG 1 cut(s) 348
AccII CGCG 2 cut(s) 419, 1502
AciI CCGC 2 cut(s) 206, 238
AclWI GGATC 4 cut(s) 930, 943, 1555, 1717
AcoI YGGCCR 2 cut(s) 2010, 2275
AcsI RAATTY 6 cut(s) 173, 659, 702, 1095, 1281, 1956
AfaI GTAC 5 cut(s) 568, 670, 986, 2153, 2221
AfiI CCNNNNNNNGG 4 cut(s) 96, 262, 348, 525
AgsI TTSAA 9 cut(s) 178, 616, 658, 664, 680, 919, 1576, 1932, 2191
AhlI ACTAGT 1 cut(s) 1483
AjnI CCWGG 4 cut(s) 94, 501, 853, 1886
AluBI AGCT 8 cut(s) 71, 585, 627, 755, 1141, 1712, 1855, 2201
AluI AGCT 8 cut(s) 71, 585, 627, 755, 1141, 1712, 1855, 2201
Alw21I GWGCWC 2 cut(s) 1524, 1857
Alw26I GTCTC 6 cut(s) 99, 323, 731, 1211, 1460, 1988
AlwI GGATC 4 cut(s) 930, 943, 1555, 1717
AlwNI CAGNNNCTG 1 cut(s) 1592
Ama87I CYCGRG 1 cut(s) 1892
AoxI GGCC 6 cut(s) 226, 649, 1889, 2010, 2054, 2275
ApoI RAATTY 6 cut(s) 173, 659, 702, 1095, 1281, 1956
ArsI GACNNNNNNTTYG 2 cut(s) 1356, 1388
Asp700I GAANNNNTTC 1 cut(s) 961
Asp718I GGTACC 1 cut(s) 2219
AspA2I CCTAGG 1 cut(s) 484
AspLEI GCGC 1 cut(s) 421
AspS9I GGNCC 4 cut(s) 226, 649, 1890, 2210
AsuC2I CCSGG 1 cut(s) 460
AsuHPI GGTGA 6 cut(s) 128, 161, 1126, 1301, 1453, 2157
AvaI CYCGRG 1 cut(s) 1892
AvaII GGWCC 1 cut(s) 2210
AvrII CCTAGG 1 cut(s) 484
BamHI GGATCC 1 cut(s) 935
BanI GGYRCC 1 cut(s) 2219
BanII GRGCYC 1 cut(s) 1857
Bbv12I GWGCWC 2 cut(s) 1524, 1857
BbvCI CCTCAGC 1 cut(s) 261
BccI CCATC 7 cut(s) 146, 217, 377, 551, 947, 1536, 2267
BceAI ACGGC 1 cut(s) 1012
BcgI CGANNNNNNTGC 2 cut(s) 1498, 1532
BciT130I CCWGG 4 cut(s) 96, 503, 855, 1888
BciVI GTATCC 3 cut(s) 425, 1292, 1766
BcnI CCSGG 1 cut(s) 460
BcoDI GTCTC 6 cut(s) 99, 323, 731, 1211, 1460, 1988
BcuI ACTAGT 1 cut(s) 1483
BfaI CTAG 8 cut(s) 38, 485, 1197, 1484, 1730, 1910, 2058, 2214
BfmI CTRYAG 6 cut(s) 328, 622, 925, 1236, 1593, 1828
BfuI GTATCC 3 cut(s) 425, 1292, 1766
BlnI CCTAGG 1 cut(s) 484
BmcAI AGTACT 1 cut(s) 2153
Bme1390I CCNGG 5 cut(s) 96, 460, 503, 855, 1888
Bme18I GGWCC 1 cut(s) 2210
BmeT110I CYCGRG 1 cut(s) 1892
BmgT120I GGNCC 4 cut(s) 226, 649, 1890, 2210
BmiI GGNNCC 2 cut(s) 937, 2221
BmrFI CCNGG 5 cut(s) 96, 460, 503, 855, 1888
BmrI ACTGGG 1 cut(s) 543
BmsI GCATC 2 cut(s) 1026, 2250
BmuI ACTGGG 1 cut(s) 543
BpmI CTGGAG 1 cut(s) 57
Bpu10I CCTNAGC 1 cut(s) 261
BpuEI CTTGAG 2 cut(s) 69, 1268
BpuMI CCSGG 1 cut(s) 460
BsaBI GATNNNNATC 1 cut(s) 1737
BsaI GGTCTC 1 cut(s) 323
BsaJI CCNNGG 6 cut(s) 95, 427, 484, 862, 1893, 2114
BsaXI ACNNNNNCTCC 2 cut(s) 1601, 1631
Bsc4I CCNNNNNNNGG 4 cut(s) 96, 262, 348, 525
Bse1I ACTGG 5 cut(s) 549, 652, 844, 1092, 2223
Bse3DI GCAATG 1 cut(s) 2082
Bse8I GATNNNNATC 1 cut(s) 1737
BseBI CCWGG 4 cut(s) 96, 503, 855, 1888
BseDI CCNNGG 6 cut(s) 95, 427, 484, 862, 1893, 2114
BseGI GGATG 6 cut(s) 290, 543, 739, 1524, 1942, 2110
BseJI GATNNNNATC 1 cut(s) 1737
BseLI CCNNNNNNNGG 4 cut(s) 96, 262, 348, 525
BseMI GCAATG 1 cut(s) 2082
BseMII CTCAG 3 cut(s) 252, 334, 2139
BseNI ACTGG 5 cut(s) 549, 652, 844, 1092, 2223
BseRI GAGGAG 2 cut(s) 348, 1609
BsgI GTGCAG 2 cut(s) 121, 2157
Bsh1236I CGCG 2 cut(s) 419, 1502
BshFI GGCC 6 cut(s) 228, 651, 1891, 2012, 2056, 2277
BshNI GGYRCC 1 cut(s) 2219
BsiHKAI GWGCWC 2 cut(s) 1524, 1857
BsiHKCI CYCGRG 1 cut(s) 1892
BsiSI CCGG 3 cut(s) 459, 519, 1328
BslFI GGGAC 3 cut(s) 960, 1358, 1777
BslI CCNNNNNNNGG 4 cut(s) 96, 262, 348, 525
BsmAI GTCTC 6 cut(s) 99, 323, 731, 1211, 1460, 1988
BsmFI GGGAC 3 cut(s) 960, 1358, 1777
BsmI GAATGC 1 cut(s) 1122
BsnI GGCC 6 cut(s) 228, 651, 1891, 2012, 2056, 2277
Bso31I GGTCTC 1 cut(s) 323
BsoBI CYCGRG 1 cut(s) 1892
Bsp1286I GDGCHC 2 cut(s) 1524, 1857
Bsp143I GATC 7 cut(s) 118, 935, 1547, 1635, 1722, 1732, 2014
Bsp19I CCATGG 1 cut(s) 2114
Bsp68I TCGCGA 1 cut(s) 1502
BspACI CCGC 2 cut(s) 206, 238
BspANI GGCC 6 cut(s) 228, 651, 1891, 2012, 2056, 2277
BspCNI CTCAG 3 cut(s) 253, 333, 2140
BspFNI CGCG 2 cut(s) 419, 1502
BspLI GGNNCC 2 cut(s) 937, 2221
BspMAI CTGCAG 4 cut(s) 626, 929, 1240, 1597
BspPI GGATC 4 cut(s) 930, 943, 1555, 1717
BspT107I GGYRCC 1 cut(s) 2219
BspTNI GGTCTC 1 cut(s) 323
BsrDI GCAATG 1 cut(s) 2082
BsrI ACTGG 5 cut(s) 549, 652, 844, 1092, 2223
BssECI CCNNGG 6 cut(s) 95, 427, 484, 862, 1893, 2114
BssMI GATC 7 cut(s) 118, 935, 1547, 1635, 1722, 1732, 2014
BssT1I CCWWGG 4 cut(s) 427, 484, 862, 2114
Bst2UI CCWGG 4 cut(s) 96, 503, 855, 1888
Bst4CI ACNGT 4 cut(s) 566, 571, 668, 2256
Bst6I CTCTTC 2 cut(s) 438, 1893
BstAPI GCANNNNNTGC 2 cut(s) 528, 1592
BstC8I GCNNGC 2 cut(s) 851, 2243
BstDEI CTNAG 5 cut(s) 261, 320, 949, 1777, 2148
BstDSI CCRYGG 1 cut(s) 2114
BstEII GGTNACC 1 cut(s) 857
BstENI CCTNNNNNAGG 1 cut(s) 260
BstF5I GGATG 6 cut(s) 290, 543, 739, 1524, 1942, 2110
BstFNI CGCG 2 cut(s) 419, 1502
BstHHI GCGC 1 cut(s) 421
BstKTI GATC 7 cut(s) 121, 938, 1550, 1638, 1725, 1735, 2017
BstMAI GTCTC 6 cut(s) 99, 323, 731, 1211, 1460, 1988
BstMBI GATC 7 cut(s) 118, 935, 1547, 1635, 1722, 1732, 2014
BstMWI GCNNNNNNNGC 5 cut(s) 74, 489, 528, 1592, 2018
BstNI CCWGG 4 cut(s) 96, 503, 855, 1888
BstNSI RCATGY 4 cut(s) 189, 469, 744, 2245
BstPI GGTNACC 1 cut(s) 857
BstSCI CCNGG 5 cut(s) 94, 458, 501, 853, 1886
BstSFI CTRYAG 6 cut(s) 328, 622, 925, 1236, 1593, 1828
BstUI CGCG 2 cut(s) 419, 1502
BstX2I RGATCY 1 cut(s) 935
BstYI RGATCY 1 cut(s) 935
BsuI GTATCC 3 cut(s) 425, 1292, 1766
BsuRI GGCC 6 cut(s) 228, 651, 1891, 2012, 2056, 2277
BtgI CCRYGG 1 cut(s) 2114
BtgZI GCGATG 1 cut(s) 1483
BtsCI GGATG 6 cut(s) 290, 543, 739, 1524, 1942, 2110
BtsIMutI CAGTG 4 cut(s) 556, 851, 1100, 1479
BtuMI TCGCGA 1 cut(s) 1502
Cac8I GCNNGC 2 cut(s) 851, 2243
CaiI CAGNNNCTG 1 cut(s) 1592
CfoI GCGC 1 cut(s) 421
Cfr13I GGNCC 4 cut(s) 226, 649, 1890, 2210
CseI GACGC 1 cut(s) 481
Csp6I GTAC 5 cut(s) 567, 669, 985, 2152, 2220
CspCI CAANNNNNGTGG 2 cut(s) 1851, 1886
CviQI GTAC 5 cut(s) 567, 669, 985, 2152, 2220
DdeI CTNAG 5 cut(s) 261, 320, 949, 1777, 2148
DpnI GATC 7 cut(s) 120, 937, 1549, 1637, 1724, 1734, 2016
DpnII GATC 7 cut(s) 118, 935, 1547, 1635, 1722, 1732, 2014
EaeI YGGCCR 2 cut(s) 2010, 2275
Eam1104I CTCTTC 2 cut(s) 438, 1893
EarI CTCTTC 2 cut(s) 438, 1893
Ecl136II GAGCTC 1 cut(s) 1855
Eco130I CCWWGG 4 cut(s) 427, 484, 862, 2114
Eco147I AGGCCT 1 cut(s) 2056
Eco24I GRGCYC 1 cut(s) 1857
Eco31I GGTCTC 1 cut(s) 323
Eco32I GATATC 1 cut(s) 2131
Eco47I GGWCC 1 cut(s) 2210
Eco53kI GAGCTC 1 cut(s) 1855
Eco88I CYCGRG 1 cut(s) 1892
Eco91I GGTNACC 1 cut(s) 857
EcoICRI GAGCTC 1 cut(s) 1855
EcoNI CCTNNNNNAGG 1 cut(s) 260
EcoO65I GGTNACC 1 cut(s) 857
EcoRI GAATTC 2 cut(s) 173, 1281
EcoRII CCWGG 4 cut(s) 94, 501, 853, 1886
EcoRV GATATC 1 cut(s) 2131
EcoT14I CCWWGG 4 cut(s) 427, 484, 862, 2114
EcoT38I GRGCYC 1 cut(s) 1857
ErhI CCWWGG 4 cut(s) 427, 484, 862, 2114
FalI AAGNNNNNCTT 4 cut(s) 1176, 1208, 1860, 1892
FaqI GGGAC 3 cut(s) 960, 1358, 1777
FokI GGATG 6 cut(s) 277, 530, 746, 1511, 1949, 2097
FriOI GRGCYC 1 cut(s) 1857
FspBI CTAG 8 cut(s) 38, 485, 1197, 1484, 1730, 1910, 2058, 2214
GlaI GCGC 1 cut(s) 420
GsuI CTGGAG 1 cut(s) 57
HaeIII GGCC 6 cut(s) 228, 651, 1891, 2012, 2056, 2277
HapII CCGG 3 cut(s) 459, 519, 1328
HgaI GACGC 1 cut(s) 481
HhaI GCGC 1 cut(s) 421
Hin6I GCGC 1 cut(s) 419
HinP1I GCGC 1 cut(s) 419
HincII GTYRAC 1 cut(s) 805
HindII GTYRAC 1 cut(s) 805
HinfI GANTC 2 cut(s) 506, 868
HpaII CCGG 3 cut(s) 459, 519, 1328
HphI GGTGA 6 cut(s) 128, 161, 1126, 1301, 1453, 2157
Hpy166II GTNNAC 5 cut(s) 554, 805, 1134, 1312, 1489
Hpy188I TCNGA 5 cut(s) 247, 323, 1702, 1709, 2176
Hpy188III TCNNGA 5 cut(s) 294, 1501, 1716, 1730, 1961
Hpy8I GTNNAC 5 cut(s) 554, 805, 1134, 1312, 1489
Hpy99I CGWCG 1 cut(s) 497
HpyAV CCTTC 6 cut(s) 173, 295, 1286, 1378, 1648, 1934
HpyCH4III ACNGT 4 cut(s) 566, 571, 668, 2256
HpyCH4IV ACGT 1 cut(s) 2007
HpyF10VI GCNNNNNNNGC 5 cut(s) 74, 489, 528, 1592, 2018
HpyF3I CTNAG 5 cut(s) 261, 320, 949, 1777, 2148
HpySE526I ACGT 1 cut(s) 2007
HspAI GCGC 1 cut(s) 419
KpnI GGTACC 1 cut(s) 2223
Kzo9I GATC 7 cut(s) 118, 935, 1547, 1635, 1722, 1732, 2014
LmnI GCTCC 7 cut(s) 76, 521, 1387, 1598, 1709, 1852, 1860
LweI GCATC 2 cut(s) 1026, 2250
MaeI CTAG 8 cut(s) 38, 485, 1197, 1484, 1730, 1910, 2058, 2214
MaeII ACGT 1 cut(s) 2007
MaeIII GTNAC 4 cut(s) 476, 833, 857, 1179
MalI GATC 7 cut(s) 120, 937, 1549, 1637, 1724, 1734, 2016
MboI GATC 7 cut(s) 118, 935, 1547, 1635, 1722, 1732, 2014
MboII GAAGA 8 cut(s) 455, 779, 1137, 1253, 1588, 1910, 1912, 2153
MfeI CAATTG 1 cut(s) 575
MflI RGATCY 1 cut(s) 935
MhlI GDGCHC 2 cut(s) 1524, 1857
MlyI GAGTC 1 cut(s) 500
MmeI TCCRAC 2 cut(s) 399, 1072
MroXI GAANNNNTTC 1 cut(s) 961
MseI TTAA 1 cut(s) 2039
MslI CAYNNNNRTG 3 cut(s) 1434, 1536, 1647
MspI CCGG 3 cut(s) 459, 519, 1328
MspR9I CCNGG 5 cut(s) 96, 460, 503, 855, 1888
MunI CAATTG 1 cut(s) 575
Mva1269I GAATGC 1 cut(s) 1122
MvaI CCWGG 4 cut(s) 96, 503, 855, 1888
MvnI CGCG 2 cut(s) 419, 1502
MwoI GCNNNNNNNGC 5 cut(s) 74, 489, 528, 1592, 2018
NciI CCSGG 1 cut(s) 460
NcoI CCATGG 1 cut(s) 2114
NdeII GATC 7 cut(s) 118, 935, 1547, 1635, 1722, 1732, 2014
NlaIV GGNNCC 2 cut(s) 937, 2221
NmeAIII GCCGAG 1 cut(s) 2139
NmuCI GTSAC 1 cut(s) 476
NruI TCGCGA 1 cut(s) 1502
NspI RCATGY 4 cut(s) 189, 469, 744, 2245
PaeI GCATGC 1 cut(s) 2245
PceI AGGCCT 1 cut(s) 2056
PctI GAATGC 1 cut(s) 1122
PdmI GAANNNNTTC 1 cut(s) 961
PfeI GAWTC 1 cut(s) 868
PflMI CCANNNNNTGG 1 cut(s) 348
PleI GAGTC 1 cut(s) 500
PpsI GAGTC 1 cut(s) 500
PsiI TTATAA 3 cut(s) 8, 591, 716
Psp124BI GAGCTC 1 cut(s) 1857
Psp6I CCWGG 4 cut(s) 94, 501, 853, 1886
PspEI GGTNACC 1 cut(s) 857
PspGI CCWGG 4 cut(s) 94, 501, 853, 1886
PspN4I GGNNCC 2 cut(s) 937, 2221
PspPI GGNCC 4 cut(s) 226, 649, 1890, 2210
PstI CTGCAG 4 cut(s) 626, 929, 1240, 1597
PstNI CAGNNNCTG 1 cut(s) 1592
PsuI RGATCY 1 cut(s) 935
RruI TCGCGA 1 cut(s) 1502
RsaI GTAC 5 cut(s) 568, 670, 986, 2153, 2221
RsaNI GTAC 5 cut(s) 567, 669, 985, 2152, 2220
RseI CAYNNNNRTG 3 cut(s) 1434, 1536, 1647
SacI GAGCTC 1 cut(s) 1857
SaqAI TTAA 1 cut(s) 2039
Sau3AI GATC 7 cut(s) 118, 935, 1547, 1635, 1722, 1732, 2014
Sau96I GGNCC 4 cut(s) 226, 649, 1890, 2210
ScaI AGTACT 1 cut(s) 2153
SchI GAGTC 1 cut(s) 500
ScrFI CCNGG 5 cut(s) 96, 460, 503, 855, 1888
SduI GDGCHC 2 cut(s) 1524, 1857
SfaNI GCATC 2 cut(s) 1026, 2250
SfcI CTRYAG 6 cut(s) 328, 622, 925, 1236, 1593, 1828
SinI GGWCC 1 cut(s) 2210
SmiMI CAYNNNNRTG 3 cut(s) 1434, 1536, 1647
SmlI CTYRAG 2 cut(s) 84, 1247
SmoI CTYRAG 2 cut(s) 84, 1247
SpeI ACTAGT 1 cut(s) 1483
SphI GCATGC 1 cut(s) 2245
SseBI AGGCCT 1 cut(s) 2056
SsiI CCGC 2 cut(s) 206, 238
SspI AATATT 3 cut(s) 51, 1323, 1990
SspMI CTAG 8 cut(s) 38, 485, 1197, 1484, 1730, 1910, 2058, 2214
SstI GAGCTC 1 cut(s) 1857
StuI AGGCCT 1 cut(s) 2056
StyD4I CCNGG 5 cut(s) 94, 458, 501, 853, 1886
StyI CCWWGG 4 cut(s) 427, 484, 862, 2114
TaaI ACNGT 4 cut(s) 566, 571, 668, 2256
TaiI ACGT 1 cut(s) 2010
TaqI TCGA 3 cut(s) 166, 495, 1518
TatI WGTACW 3 cut(s) 566, 668, 2151
TauI GCSGC 1 cut(s) 208
TfiI GAWTC 1 cut(s) 868
Tru1I TTAA 1 cut(s) 2039
Tru9I TTAA 1 cut(s) 2039
TscAI CASTG 4 cut(s) 556, 851, 1107, 1479
TseFI GTSAC 1 cut(s) 476
Tsp45I GTSAC 1 cut(s) 476
TspDTI ATGAA 9 cut(s) 442, 592, 625, 1044, 1287, 1486, 1548, 1953, 2078
TspGWI ACGGA 1 cut(s) 1317
TspRI CASTG 4 cut(s) 556, 851, 1107, 1479
Van91I CCANNNNNTGG 1 cut(s) 348
VpaK11BI GGWCC 1 cut(s) 2210
XagI CCTNNNNNAGG 1 cut(s) 260
XapI RAATTY 6 cut(s) 173, 659, 702, 1095, 1281, 1956
XbaI TCTAGA 1 cut(s) 1729
XceI RCATGY 4 cut(s) 189, 469, 744, 2245
XcmI CCANNNNNNNNNTGG 1 cut(s) 1535
XmaJI CCTAGG 1 cut(s) 484
XmnI GAANNNNTTC 1 cut(s) 961
XspI CTAG 8 cut(s) 38, 485, 1197, 1484, 1730, 1910, 2058, 2214
ZrmI AGTACT 1 cut(s) 2153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.