MD15G1088400.v1.1

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000066
Physical Location & Seq
Reverse (-)
6166287 .. 6168868
2582 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1088400.v1.1.491

Sequence Viewer

Length: 2343 bp
ATGCTCATGCAACTCACCTTAATAGAGCTACTGCTACTAGCAGCAGCCACTTCATCCCAAGCCACACAATTCATCCCTCCTCCTCCGTCGTCTCCGCCCCTTGATCAAGTTGGAGTCAAGCCGGGTTGCCAAGCCAAATGTGGGAATGTTAGCATCCCATATCCATTTGGTACAACCCCAGATTGTTACTACAACGAGGATTTCCTTATTACTTGCAATGACACACACTACAACCCTCCAAAGCCATTTCTTGGAGATAGTAACATTGACGTCACAAACATATCTGTCGACGGGAAGCTGCATATTCTACAATATATAGCCAAAGATTGTTACAACGAATCAGGTGTGTCCACGGAAAGCAACACTCCTTATATCCAACTGCCCAACTTCTTCATATCTGACACCGATAACGTGTTTGTGGTGGTTGGTTGCGACACCACCGCTGAAATTGTGGTTTTCCAAGGAGAAGATTATGGGTACACAGGTGGGTGCATTACAAAATGTGACAGCATTAATTTTGTTGCCAATGATTCGTGCTCCGGTGTAGGCTGTTGCCAAACATCGATCGCTAAAAATACGGATTATTATCAAATAAGCGTGAATAGTTATAATAATCACAAGGGTGTGTGGGACTTCAATCCCTGCAGCTATGCCTTTGTTGTCGAACAGAGCAAGTTCAACTTCTCTTCGAATCTGCTGACCGATTTGAACGACGTGGAAGAGCTTCCGGTGGTTCTAGATTGGTCAATTGGGAATGAGACATGCAGCAAGGTTGTTGGAAACAAACAAGTGATGAATTACGCATGTCATGGGAACGAAACATGTATCGATGTCAAGAATGGTGGGGGTTATCGCTGCGAGTGCCAAAACGGTTACCGAGGGAATCCATACCTTGAGGGATGCCATGGACTACTACTAATTAATACAACTTGCTTTCTGAAAATTATGGGTCATGTTATTTTTGCAGACATTAATGAATGTGAGGATGCAAAACTCAATAACTGCGAACAGATATGTACTAATACAGATGGAAGCTATGCTTGTTCTTGCCGCAAGGGGTACTATGGCGATGGTAGAGTAGACGGAGAAGGTTGCACCCATAAACCAACATTACATATTCAGATTATCATTGGAATCGGGGTTGGCCTCATAGCCTTGCTAATGGGAAGCTCTTGGTTGTATTTGGGCTACAAAAGATGGAAGCTCATAAGGCTGAAGGAGAAGTTCTTCAGGCAAAATGGAGGACTAATGCTACAACAACAACTTTCAGAACGACAGGGATCCACTCGCGAAACAGCCAAAATCTTCACAGCAGAAGAACTTGAGAAGGCCACAAATAATTACAATGAGTCTAAAATCATTGGCAGAGGAGGTTCCGGTACAGTGTACAAAGGAATTTTAGTAGACGGCCGAGTGGTTGCTATCAAGAAGTCCAAAATGATGGATCAAAGCCAAATAGAGCAATTCATAAATGAGGTACTGGTGCTTTCACAGATCAACCATAGGAATGTGGTTAAGCTTTTAGGTTGTTGTTTCGAGACACAAGTTCCACTGCTAGTCTATGAGTATGTAACAAATGGAACACTCTCTGACTACATCCATAATACAACCAAGGAATCCAAACCAAACACCTCGTGGGAAATCCATCTAAAGATAGCTTCAGAAACAGCTGGAGTGCTGTCATACTTGCACTCTGCGGCTTCTGTTCCTATCATTCACAGAGATGTCAAGTCTACAAACATACTCTTAGATGAAAACCTCACAGCCAAAGTGTCTGACTTTGGAGCCTCGAGATTGGTTCCGTTAGGCCAAGATCAGTTGTCCACAATGGTGCAAGGAACTATGGGGTATTTAGACCCCGAATACATGCAGACAAGCCAATTGACTGAGAAAAGCGATGTCTATAGTTTCGGGGTTGTCCTCGTAGAGCTATTGACAAGAAAGAAGGCGGTTTTCTTTGACAAGCCGCAGGAAAAGATAAATTTGGCTATGTATTTTCTGTCAGCATTGAAAGATGACCGATTGCTGCAAGTTCTAGATGATTGGATTGTGACTGAGGCAAACATTGAGCAGCTTAAAGAAGTTTCTAAACTTGCAATGAGGTGTTTGAGAGTAAAAGGGGAAGAAAGACCAACGATGCAGGAAGTTGCTATGGAACTAGAGGGACTGAGAAGGATGGTATTGCATCCATGGGTGAATGATGAATTCATTTCAGAAGAGACTCAGCACTTGCTTGGTGGAATATCAATGGAAACTATTAGTTATGAAGGAGGTGGCGATTCAGGTTTAGGGTATGATACAATGAGGAACCACGTCGCATTACCAGTTAATGGTGGGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF_CA GUB_WAK_bind Pkinase WAK

Protein Analysis

781

Amino Acids

86.58

Weight (kDa)

5.12

Isoelectric Point (pI)

34.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 43 - 98 6e-10 Wall-associated receptor kinase galacturonan-binding
EGF_CA PF07645 323 - 355 1.3e-07 Calcium-binding EGF domain
Pkinase PF00069 448 - 716 8.4e-48 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 451 - 720 2.1e-47 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21210 AT1G21230 AT1G21240 AT1G21240 AT1G21245 AT1G21250 AT1G21270 AT1G22720 AT3G25490
fragaria_vesca FvH4_2g35051 FvH4_2g35051 FvH4_2g35060 FvH4_3g10281 FvH4_3g10285 FvH4_3g10286 FvH4_3g10311 FvH4_3g10311 FvH4_3g10320 FvH4_3g10332 FvH4_3g10340 FvH4_3g10350 FvH4_3g10360 FvH4_3g10390 FvH4_3g10412 FvH4_3g10413 FvH4_3g10461 FvH4_3g10462 FvH4_3g10470 FvH4_3g10510 FvH4_3g10520 FvH4_3g10530 FvH4_3g10550 FvH4_3g10560 FvH4_3g10611 FvH4_3g11730 FvH4_6g53140 FvH4_6g53142 FvH4_7g31880 FvH4_7g31910 FvH4_7g31920
malus_domestica MD05G1269900.v1.1 MD05G1270000.v1.1 MD05G1270200.v1.1 MD05G1270400.v1.1 MD05G1270500.v1.1 MD05G1270600.v1.1 MD05G1270800.v1.1 MD05G1270900.v1.1 MD07G1183800.v1.1 MD08G1107600.v1.1 MD10G1248500.v1.1 MD10G1249200.v1.1 MD10G1249500.v1.1 MD10G1250000.v1.1 MD10G1250500.v1.1 MD10G1250900.v1.1 MD10G1251200.v1.1 MD10G1251400.v1.1 MD13G1004600.v1.1 MD15G1088400.v1.1
prunus_persica Prupe.1G442400_v2.0.a1 Prupe.1G442500_v2.0.a1 Prupe.2G223400_v2.0.a1 Prupe.4G091900_v2.0.a1 Prupe.4G093200_v2.0.a1 Prupe.4G093300_v2.0.a1 Prupe.4G093400_v2.0.a1 Prupe.4G093500_v2.0.a1 Prupe.4G094100_v2.0.a1 Prupe.4G094200_v2.0.a1 Prupe.4G094300_v2.0.a1 Prupe.6G358300_v2.0.a1
pyrus_communis pycom08g08990 pycom10g20660 pycom10g20670 pycom10g20680 pycom10g20710 pycom10g20740 pycom13g00400 pycom15g08290
rosa_chinensis RchiOBHm_Chr1g0326861 RchiOBHm_Chr1g0326871 RchiOBHm_Chr1g0331391 RchiOBHm_Chr1g0336911 RchiOBHm_Chr1g0364001 RchiOBHm_Chr2g0174541 RchiOBHm_Chr2g0175041 RchiOBHm_Chr2g0175101 RchiOBHm_Chr2g0175121 RchiOBHm_Chr3g0486331 RchiOBHm_Chr5g0015581 RchiOBHm_Chr5g0016551 RchiOBHm_Chr5g0016561 RchiOBHm_Chr5g0016581 RchiOBHm_Chr5g0016601 RchiOBHm_Chr5g0016611 RchiOBHm_Chr5g0016641 RchiOBHm_Chr5g0016661 RchiOBHm_Chr5g0016691 RchiOBHm_Chr5g0016701 RchiOBHm_Chr5g0016731 RchiOBHm_Chr5g0016741 RchiOBHm_Chr5g0016811 RchiOBHm_Chr5g0016841 RchiOBHm_Chr5g0016871 RchiOBHm_Chr5g0016881 RchiOBHm_Chr5g0016931 RchiOBHm_Chr5g0017021 RchiOBHm_Chr5g0017041 RchiOBHm_Chr5g0017071 RchiOBHm_Chr5g0017091 RchiOBHm_Chr5g0017121 RchiOBHm_Chr5g0017141 RchiOBHm_Chr5g0017291 RchiOBHm_Chr5g0017321 RchiOBHm_Chr5g0017401 RchiOBHm_Chr5g0019001 RchiOBHm_Chr5g0019071 RchiOBHm_Chr5g0028941 RchiOBHm_Chr5g0052141 RchiOBHm_Chr5g0054721 RchiOBHm_Chr5g0054801 RchiOBHm_Chr5g0054931 RchiOBHm_Chr5g0054941 RchiOBHm_Chr6g0259291 RchiOBHm_Chr6g0292991 RchiOBHm_Chr6g0306911
rosa_laevigata RLG00000009465 RLG00000009468 RLG00000010755 RLG00000011976 RLG00000014541 RLG00000014542 RLG00000015169 RLG00000022308 RLG00000027529 RLG00000029324 RLG00000030244 RLG00000030245 RLG00000032210 RLG00000032211 RLG00000032257 RLG00000032259 RLG00000032260 RLG00000032261 RLG00000032262 RLG00000032268 RLG00000032269 RLG00000032272 RLG00000032282 RLG00000032283 RLG00000032308 RLG00000032413 RLG00000033129 RLG00000034945
rosa_multiflora Rmu_co8241929.1_g000001 Rmu_co8269227.1_g000001 Rmu_co8461259.1_g000001 Rmu_co8495385.1_g000001 Rmu_sc0000028.1_g000003 Rmu_sc0000083.1_g000009 Rmu_sc0000083.1_g000010 Rmu_sc0000083.1_g000031 Rmu_sc0000083.1_g000043 Rmu_sc0000083.1_g000046 Rmu_sc0000083.1_g000047 Rmu_sc0000563.1_g000024 Rmu_sc0000842.1_g000012 Rmu_sc0001235.1_g000001 Rmu_sc0001235.1_g000005 Rmu_sc0001235.1_g000009 Rmu_sc0001235.1_g000015 Rmu_sc0001235.1_g000016 Rmu_sc0001235.1_g000017 Rmu_sc0001473.1_g000036 Rmu_sc0001607.1_g000016 Rmu_sc0001788.1_g000006 Rmu_sc0002018.1_g000008 Rmu_sc0002028.1_g000001 Rmu_sc0002680.1_g000011 Rmu_sc0002798.1_g000023 Rmu_sc0002886.1_g000023 Rmu_sc0002989.1_g000012 Rmu_sc0003064.1_g000009 Rmu_sc0003382.1_g000006 Rmu_sc0003630.1_g000002 Rmu_sc0003630.1_g000047 Rmu_sc0003630.1_g000066 Rmu_sc0003630.1_g000067 Rmu_sc0003649.1_g000010 Rmu_sc0003688.1_g000005 Rmu_sc0003920.1_g000007 Rmu_sc0004765.1_g000007 Rmu_sc0004765.1_g000008 Rmu_sc0004920.1_g000011 Rmu_sc0005223.1_g000008 Rmu_sc0005223.1_g000010 Rmu_sc0005223.1_g000015 Rmu_sc0005223.1_g000016 Rmu_sc0005319.1_g000014 Rmu_sc0005319.1_g000015 Rmu_sc0005319.1_g000018 Rmu_sc0005319.1_g000019 Rmu_sc0005319.1_g000025 Rmu_sc0005418.1_g000007 Rmu_sc0005418.1_g000012 Rmu_sc0006106.1_g000011 Rmu_sc0006914.1_g000012 Rmu_sc0007023.1_g000001 Rmu_sc0007662.1_g000001 Rmu_sc0007920.1_g000002 Rmu_sc0007920.1_g000013 Rmu_sc0008053.1_g000016 Rmu_sc0008053.1_g000017 Rmu_sc0008053.1_g000018 Rmu_sc0008053.1_g000023 Rmu_sc0008053.1_g000050 Rmu_sc0009042.1_g000007 Rmu_sc0009042.1_g000008 Rmu_sc0010642.1_g000008 Rmu_sc0011109.1_g000002 Rmu_sc0011314.1_g000006 Rmu_sc0011974.1_g000006 Rmu_sc0012179.1_g000009 Rmu_sc0015169.1_g000001 Rmu_sc0016368.1_g000002 Rmu_sc0022659.1_g000001 Rmu_sc0027371.1_g000001 Rmu_sc0028213.1_g000001 Rmu_sc0034526.1_g000001 Rmu_sc0039366.1_g000002 Rmu_ssc0000050.1_g000084 Rmu_ssc0000050.1_g000088 Rmu_ssc0000167.1_g000017
rosa_roxburghii Rroxscaffold_1G00011340 Rroxscaffold_1G00025710 Rroxscaffold_1G00025870 Rroxscaffold_1G00026070 Rroxscaffold_1G00026170 Rroxscaffold_1G00026200 Rroxscaffold_1G00026270 Rroxscaffold_1G00050950 Rroxscaffold_1G00058900 Rroxscaffold_1G00060040 Rroxscaffold_1G00060060 Rroxscaffold_1G00060070 Rroxscaffold_1G00060080 Rroxscaffold_1G00060110 Rroxscaffold_1G00060120 Rroxscaffold_1G00060140 Rroxscaffold_1G00060170 Rroxscaffold_1G00060210 Rroxscaffold_1G00060220 Rroxscaffold_1G00060240 Rroxscaffold_1G00060250 Rroxscaffold_1G00060260 Rroxscaffold_1G00060300 Rroxscaffold_1G00060320 Rroxscaffold_1G00060330 Rroxscaffold_1G00060900 Rroxscaffold_1G00060920 Rroxscaffold_1G00073980 Rroxscaffold_2G00077560 Rroxscaffold_2G00077580 Rroxscaffold_2G00146990 Rroxscaffold_3G00236200 Rroxscaffold_3G00236210 Rroxscaffold_3G00236220 Rroxscaffold_4G00292760 Rroxscaffold_4G00315200 Rroxscaffold_4G00315240 Rroxscaffold_4G00326240 Rroxscaffold_4G00328980 Rroxscaffold_5G00341890 Rroxscaffold_7G00161350 Rroxscaffold_7G00174490 Rroxscaffold_7G00206550
rosa_rugosa Rorug01G0093000 Rorug01G0093100 Rorug01G0128800 Rorug01G0312000 Rorug01G0312000 Rorug02G0581100 Rorug03G0224600 Rorug05G0028500 Rorug05G0032700 Rorug05G0032800 Rorug05G0032900 Rorug05G0032900 Rorug05G0033000 Rorug05G0033100 Rorug05G0033300 Rorug05G0033400 Rorug05G0033900 Rorug05G0034900 Rorug05G0035000 Rorug05G0046900 Rorug05G0047500 Rorug05G0047500 Rorug05G0111600 Rorug05G0287900 Rorug05G0288700 Rorug05G0586900 Rorug06G0231700 Rorug06G0354300 Rorug06G0354400
rosa_samantha Rh1DG122200 Rh2DG686800 Rh5BG136600 Rh6AG344500 Rh6BG351900 Rh6CG359000 Rh6CG359100 Rh6CG387600 Rh6CG480500 Rh6DG344600 Rh6DG467400
rosa_wichuraiana Rw0G010250 Rw0G014820 Rw0G021400 Rw0G023100 Rw1G009450 Rw1G012100 Rw1G012180 Rw1G028360 Rw2G054260 Rw3G024370 Rw3G024420 Rw3G024470 Rw5G010850 Rw5G011010 Rw5G011020 Rw5G011050 Rw5G011080 Rw5G011120 Rw5G012300 Rw5G018470 Rw5G033830 Rw6G008930 Rw6G030070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 609
AatII GACGTC 1 cut(s) 273
AccB7I CCANNNNNTGG 2 cut(s) 251, 2330
AccI GTMKAC 4 cut(s) 288, 1080, 1404, 1733
AccII CGCG 1 cut(s) 1290
AciI CCGC 6 cut(s) 95, 441, 1051, 1697, 1949, 1967
AclWI GGATC 3 cut(s) 1275, 1288, 1452
AcoI YGGCCR 1 cut(s) 1408
AcsI RAATTY 3 cut(s) 1395, 1981, 2204
AcuI CTGAAG 3 cut(s) 1213, 1235, 1644
AcyI GRCGYC 1 cut(s) 270
AdeI CACNNNGTG 1 cut(s) 1635
AfaI GTAC 7 cut(s) 172, 479, 1018, 1061, 1381, 1388, 1479
AfiI CCNNNNNNNGG 3 cut(s) 141, 251, 2330
AflIII ACRYGT 2 cut(s) 411, 821
AgsI TTSAA 4 cut(s) 637, 679, 709, 2011
AjiI CACGTC 2 cut(s) 715, 2314
AleI CACNNNNGTG 2 cut(s) 621, 1829
Alw21I GWGCWC 1 cut(s) 539
Alw26I GTCTC 4 cut(s) 96, 752, 1532, 2213
AlwI GGATC 3 cut(s) 1275, 1288, 1452
Ama87I CYCGRG 1 cut(s) 1789
AoxI GGCC 4 cut(s) 1144, 1329, 1408, 1807
ApeKI GCWGC 8 cut(s) 41, 44, 298, 645, 765, 855, 2026, 2071
ApoI RAATTY 3 cut(s) 1395, 1981, 2204
AseI ATTAAT 3 cut(s) 513, 921, 972
Asp700I GAANNNNTTC 2 cut(s) 723, 1226
AsuC2I CCSGG 1 cut(s) 123
AsuHPI GGTGA 2 cut(s) 7, 2206
AsuII TTCGAA 1 cut(s) 689
AvaI CYCGRG 1 cut(s) 1789
BaeI ACNNNNGTAYC 2 cut(s) 469, 502
BamHI GGATCC 1 cut(s) 1280
BauI CACGAG 1 cut(s) 1633
Bbv12I GWGCWC 1 cut(s) 539
BbvI GCAGC 8 cut(s) 53, 56, 285, 657, 777, 842, 2013, 2083
BccI CCATC 6 cut(s) 1022, 1064, 1191, 1435, 1653, 2170
BceAI ACGGC 1 cut(s) 1423
BclI TGATCA 1 cut(s) 103
BcnI CCSGG 1 cut(s) 123
BcoDI GTCTC 4 cut(s) 96, 752, 1532, 2213
BfaI CTAG 5 cut(s) 38, 737, 1556, 2036, 2159
BfmI CTRYAG 2 cut(s) 643, 1903
Bme1390I CCNGG 1 cut(s) 123
BmeT110I CYCGRG 1 cut(s) 1789
BmgBI CACGTC 2 cut(s) 715, 2314
BmiI GGNNCC 5 cut(s) 1282, 1375, 1786, 1800, 2309
BmrFI CCNGG 1 cut(s) 123
BmsI GCATC 5 cut(s) 162, 890, 976, 2127, 2194
BpmI CTGGAG 1 cut(s) 1692
Bpu14I TTCGAA 1 cut(s) 689
BpuEI CTTGAG 2 cut(s) 914, 1343
BpuMI CCSGG 1 cut(s) 123
Bsa29I ATCGAT 2 cut(s) 563, 828
BsaBI GATNNNNATC 1 cut(s) 585
BsaHI GRCGYC 1 cut(s) 270
BsaJI CCNNGG 6 cut(s) 351, 460, 877, 904, 1611, 2189
BsaWI WCCGGW 3 cut(s) 539, 727, 1376
Bsc4I CCNNNNNNNGG 3 cut(s) 141, 251, 2330
Bse1I ACTGG 2 cut(s) 1485, 2324
Bse3DI GCAATG 2 cut(s) 223, 2103
Bse8I GATNNNNATC 1 cut(s) 585
BseCI ATCGAT 2 cut(s) 563, 828
BseDI CCNNGG 6 cut(s) 351, 460, 877, 904, 1611, 2189
BseGI GGATG 8 cut(s) 53, 72, 153, 905, 991, 1596, 2181, 2185
BseJI GATNNNNATC 1 cut(s) 585
BseLI CCNNNNNNNGG 3 cut(s) 141, 251, 2330
BseMI GCAATG 2 cut(s) 223, 2103
BseMII CTCAG 4 cut(s) 1878, 2046, 2159, 2237
BseNI ACTGG 2 cut(s) 1485, 2324
BseRI GAGGAG 3 cut(s) 69, 72, 1383
BseX3I CGGCCG 1 cut(s) 1408
BseXI GCAGC 8 cut(s) 53, 56, 285, 657, 777, 842, 2013, 2083
Bsh1236I CGCG 1 cut(s) 1290
Bsh1285I CGRYCG 2 cut(s) 567, 1411
BshFI GGCC 4 cut(s) 1146, 1331, 1410, 1809
BshVI ATCGAT 2 cut(s) 563, 828
BsiEI CGRYCG 2 cut(s) 567, 1411
BsiHKAI GWGCWC 1 cut(s) 539
BsiHKCI CYCGRG 1 cut(s) 1789
BsiSI CCGG 4 cut(s) 122, 540, 728, 1377
BslFI GGGAC 2 cut(s) 644, 2178
BslI CCNNNNNNNGG 3 cut(s) 141, 251, 2330
BsmAI GTCTC 4 cut(s) 96, 752, 1532, 2213
BsmBI CGTCTC 1 cut(s) 96
BsmFI GGGAC 2 cut(s) 644, 2178
BsnI GGCC 4 cut(s) 1146, 1331, 1410, 1809
BsoBI CYCGRG 1 cut(s) 1789
Bsp119I TTCGAA 1 cut(s) 689
Bsp1286I GDGCHC 1 cut(s) 539
Bsp1407I TGTACA 1 cut(s) 1386
Bsp143I GATC 6 cut(s) 103, 564, 1280, 1444, 1494, 1813
Bsp19I CCATGG 2 cut(s) 904, 2189
Bsp68I TCGCGA 1 cut(s) 1290
BspACI CCGC 6 cut(s) 95, 441, 1051, 1697, 1949, 1967
BspANI GGCC 4 cut(s) 1146, 1331, 1410, 1809
BspCNI CTCAG 4 cut(s) 1879, 2047, 2160, 2236
BspDI ATCGAT 2 cut(s) 563, 828
BspFNI CGCG 1 cut(s) 1290
BspLI GGNNCC 5 cut(s) 1282, 1375, 1786, 1800, 2309
BspMAI CTGCAG 1 cut(s) 647
BspPI GGATC 3 cut(s) 1275, 1288, 1452
BspQI GCTCTTC 1 cut(s) 714
BspT104I TTCGAA 1 cut(s) 689
BsrDI GCAATG 2 cut(s) 223, 2103
BsrGI TGTACA 1 cut(s) 1386
BsrI ACTGG 2 cut(s) 1485, 2324
BssECI CCNNGG 6 cut(s) 351, 460, 877, 904, 1611, 2189
BssMI GATC 6 cut(s) 103, 564, 1280, 1444, 1494, 1813
BssNI GRCGYC 1 cut(s) 270
BssSI CACGAG 1 cut(s) 1633
BssT1I CCWWGG 4 cut(s) 460, 904, 1611, 2189
Bst2BI CACGAG 1 cut(s) 1633
Bst4CI ACNGT 2 cut(s) 872, 1384
Bst6I CTCTTC 3 cut(s) 691, 714, 2211
BstACI GRCGYC 1 cut(s) 270
BstAUI TGTACA 1 cut(s) 1386
BstBI TTCGAA 1 cut(s) 689
BstDEI CTNAG 5 cut(s) 1747, 1887, 2055, 2168, 2223
BstDSI CCRYGG 3 cut(s) 351, 904, 2189
BstEII GGTNACC 1 cut(s) 872
BstF5I GGATG 8 cut(s) 53, 72, 153, 905, 991, 1596, 2181, 2185
BstFNI CGCG 1 cut(s) 1290
BstKTI GATC 6 cut(s) 106, 567, 1283, 1447, 1497, 1816
BstMAI GTCTC 4 cut(s) 96, 752, 1532, 2213
BstMBI GATC 6 cut(s) 103, 564, 1280, 1444, 1494, 1813
BstMCI CGRYCG 2 cut(s) 567, 1411
BstMWI GCNNNNNNNGC 2 cut(s) 861, 1210
BstNSI RCATGY 4 cut(s) 765, 807, 825, 1870
BstPI GGTNACC 1 cut(s) 872
BstSCI CCNGG 1 cut(s) 121
BstSFI CTRYAG 2 cut(s) 643, 1903
BstUI CGCG 1 cut(s) 1290
BstV1I GCAGC 8 cut(s) 53, 56, 285, 657, 777, 842, 2013, 2083
BstX2I RGATCY 1 cut(s) 1280
BstXI CCANNNNNNTGG 1 cut(s) 1441
BstYI RGATCY 1 cut(s) 1280
BstZI CGGCCG 1 cut(s) 1408
Bsu15I ATCGAT 2 cut(s) 563, 828
BsuRI GGCC 4 cut(s) 1146, 1331, 1410, 1809
BsuTUI ATCGAT 2 cut(s) 563, 828
BtgI CCRYGG 3 cut(s) 351, 904, 2189
BtgZI GCGATG 2 cut(s) 1083, 1911
BtrI CACGTC 2 cut(s) 715, 2314
BtsCI GGATG 8 cut(s) 53, 72, 153, 905, 991, 1596, 2181, 2185
BtsI GCAGTG 1 cut(s) 1550
BtsIMutI CAGTG 2 cut(s) 1389, 1550
BtuMI TCGCGA 1 cut(s) 1290
ClaI ATCGAT 2 cut(s) 563, 828
Csp6I GTAC 7 cut(s) 171, 478, 1017, 1060, 1380, 1387, 1478
CspCI CAANNNNNGTGG 2 cut(s) 823, 858
CviAII CATG 9 cut(s) 7, 762, 804, 809, 822, 905, 953, 1867, 2190
CviQI GTAC 7 cut(s) 171, 478, 1017, 1060, 1380, 1387, 1478
DdeI CTNAG 5 cut(s) 1747, 1887, 2055, 2168, 2223
DpnI GATC 6 cut(s) 105, 566, 1282, 1446, 1496, 1815
DpnII GATC 6 cut(s) 103, 564, 1280, 1444, 1494, 1813
DraIII CACNNNGTG 1 cut(s) 1635
EaeI YGGCCR 1 cut(s) 1408
EagI CGGCCG 1 cut(s) 1408
Eam1104I CTCTTC 3 cut(s) 691, 714, 2211
EarI CTCTTC 3 cut(s) 691, 714, 2211
EciI GGCGGA 1 cut(s) 84
EclXI CGGCCG 1 cut(s) 1408
Eco130I CCWWGG 4 cut(s) 460, 904, 1611, 2189
Eco52I CGGCCG 1 cut(s) 1408
Eco57I CTGAAG 3 cut(s) 1213, 1235, 1644
Eco88I CYCGRG 1 cut(s) 1789
Eco91I GGTNACC 1 cut(s) 872
EcoO65I GGTNACC 1 cut(s) 872
EcoRI GAATTC 1 cut(s) 2204
EcoT14I CCWWGG 4 cut(s) 460, 904, 1611, 2189
ErhI CCWWGG 4 cut(s) 460, 904, 1611, 2189
Esp3I CGTCTC 1 cut(s) 96
FaeI CATG 9 cut(s) 10, 765, 807, 812, 825, 908, 956, 1870, 2193
FalI AAGNNNNNCTT 4 cut(s) 665, 697, 1024, 1056
FaqI GGGAC 2 cut(s) 644, 2178
FatI CATG 9 cut(s) 6, 761, 803, 808, 821, 904, 952, 1866, 2189
FbaI TGATCA 1 cut(s) 103
FblI GTMKAC 4 cut(s) 288, 1080, 1404, 1733
FokI GGATG 8 cut(s) 40, 59, 140, 912, 998, 1583, 2172, 2188
FspBI CTAG 5 cut(s) 38, 737, 1556, 2036, 2159
GsuI CTGGAG 1 cut(s) 1692
HaeIII GGCC 4 cut(s) 1146, 1331, 1410, 1809
HapII CCGG 4 cut(s) 122, 540, 728, 1377
Hin1I GRCGYC 1 cut(s) 270
Hin1II CATG 9 cut(s) 10, 765, 807, 812, 825, 908, 956, 1870, 2193
HincII GTYRAC 1 cut(s) 289
HindII GTYRAC 1 cut(s) 289
HindIII AAGCTT 1 cut(s) 1517
HpaII CCGG 4 cut(s) 122, 540, 728, 1377
HphI GGTGA 2 cut(s) 7, 2206
Hpy166II GTNNAC 8 cut(s) 289, 351, 480, 1081, 1387, 1405, 1734, 1824
Hpy188I TCNGA 8 cut(s) 400, 939, 1122, 1270, 1591, 1663, 1777, 2215
Hpy188III TCNNGA 7 cut(s) 737, 835, 1289, 1426, 1537, 1791, 2036
Hpy8I GTNNAC 8 cut(s) 289, 351, 480, 1081, 1387, 1405, 1734, 1824
Hpy99I CGWCG 4 cut(s) 91, 293, 716, 2318
HpyAV CCTTC 6 cut(s) 1082, 1210, 1321, 1939, 2166, 2261
HpyCH4III ACNGT 2 cut(s) 872, 1384
HpyCH4IV ACGT 4 cut(s) 270, 411, 714, 2313
HpyF10VI GCNNNNNNNGC 2 cut(s) 861, 1210
HpyF3I CTNAG 5 cut(s) 1747, 1887, 2055, 2168, 2223
HpySE526I ACGT 4 cut(s) 270, 411, 714, 2313
Hsp92I GRCGYC 1 cut(s) 270
Hsp92II CATG 9 cut(s) 10, 765, 807, 812, 825, 908, 956, 1870, 2193
Ksp22I TGATCA 1 cut(s) 103
Kzo9I GATC 6 cut(s) 103, 564, 1280, 1444, 1494, 1813
LguI GCTCTTC 1 cut(s) 714
LmnI GCTCC 2 cut(s) 542, 1784
Lsp1109I GCAGC 8 cut(s) 53, 56, 285, 657, 777, 842, 2013, 2083
LweI GCATC 5 cut(s) 162, 890, 976, 2127, 2194
MaeI CTAG 5 cut(s) 38, 737, 1556, 2036, 2159
MaeII ACGT 4 cut(s) 270, 411, 714, 2313
MaeIII GTNAC 8 cut(s) 185, 260, 271, 329, 503, 872, 1570, 2050
MalI GATC 6 cut(s) 105, 566, 1282, 1446, 1496, 1815
MboI GATC 6 cut(s) 103, 564, 1280, 1444, 1494, 1813
MboII GAAGA 9 cut(s) 382, 479, 678, 731, 1219, 1297, 1328, 2135, 2228
MfeI CAATTG 2 cut(s) 747, 1880
MflI RGATCY 1 cut(s) 1280
MhlI GDGCHC 1 cut(s) 539
MlyI GAGTC 3 cut(s) 123, 1358, 2215
MmeI TCCRAC 3 cut(s) 91, 400, 757
MroXI GAANNNNTTC 2 cut(s) 723, 1226
MseI TTAA 7 cut(s) 20, 513, 921, 972, 1515, 2076, 2328
MslI CAYNNNNRTG 4 cut(s) 621, 1506, 1722, 1829
MspA1I CMGCKG 2 cut(s) 443, 1670
MspI CCGG 4 cut(s) 122, 540, 728, 1377
MspR9I CCNGG 1 cut(s) 123
MunI CAATTG 2 cut(s) 747, 1880
MvnI CGCG 1 cut(s) 1290
MwoI GCNNNNNNNGC 2 cut(s) 861, 1210
NciI CCSGG 1 cut(s) 123
NcoI CCATGG 2 cut(s) 904, 2189
NdeII GATC 6 cut(s) 103, 564, 1280, 1444, 1494, 1813
NlaIII CATG 9 cut(s) 10, 765, 807, 812, 825, 908, 956, 1870, 2193
NlaIV GGNNCC 5 cut(s) 1282, 1375, 1786, 1800, 2309
NmeAIII GCCGAG 1 cut(s) 1436
NmuCI GTSAC 3 cut(s) 271, 503, 2050
NruI TCGCGA 1 cut(s) 1290
NspI RCATGY 4 cut(s) 765, 807, 825, 1870
NspV TTCGAA 1 cut(s) 689
OliI CACNNNNGTG 2 cut(s) 621, 1829
PaeR7I CTCGAG 1 cut(s) 1789
PciI ACATGT 1 cut(s) 821
PciSI GCTCTTC 1 cut(s) 714
PdmI GAANNNNTTC 2 cut(s) 723, 1226
PfeI GAWTC 7 cut(s) 338, 530, 691, 883, 1134, 1616, 2279
PflMI CCANNNNNTGG 2 cut(s) 251, 2330
Ple19I CGATCG 1 cut(s) 567
PleI GAGTC 3 cut(s) 122, 1357, 2215
PpsI GAGTC 3 cut(s) 122, 1357, 2215
PscI ACATGT 1 cut(s) 821
PshBI ATTAAT 3 cut(s) 513, 921, 972
PsiI TTATAA 1 cut(s) 609
PspEI GGTNACC 1 cut(s) 872
PspN4I GGNNCC 5 cut(s) 1282, 1375, 1786, 1800, 2309
PstI CTGCAG 1 cut(s) 647
PsuI RGATCY 1 cut(s) 1280
PvuI CGATCG 1 cut(s) 567
PvuII CAGCTG 1 cut(s) 1670
RruI TCGCGA 1 cut(s) 1290
RsaI GTAC 7 cut(s) 172, 479, 1018, 1061, 1381, 1388, 1479
RsaNI GTAC 7 cut(s) 171, 478, 1017, 1060, 1380, 1387, 1478
RseI CAYNNNNRTG 4 cut(s) 621, 1506, 1722, 1829
SalI GTCGAC 1 cut(s) 287
SapI GCTCTTC 1 cut(s) 714
SaqAI TTAA 7 cut(s) 20, 513, 921, 972, 1515, 2076, 2328
Sau3AI GATC 6 cut(s) 103, 564, 1280, 1444, 1494, 1813
SchI GAGTC 3 cut(s) 123, 1358, 2215
ScrFI CCNGG 1 cut(s) 123
SduI GDGCHC 1 cut(s) 539
SfaNI GCATC 5 cut(s) 162, 890, 976, 2127, 2194
SfcI CTRYAG 2 cut(s) 643, 1903
Sfr274I CTCGAG 1 cut(s) 1789
SfuI TTCGAA 1 cut(s) 689
SlaI CTCGAG 1 cut(s) 1789
SmiMI CAYNNNNRTG 4 cut(s) 621, 1506, 1722, 1829
SmlI CTYRAG 3 cut(s) 893, 1322, 1789
SmoI CTYRAG 3 cut(s) 893, 1322, 1789
SsiI CCGC 6 cut(s) 95, 441, 1051, 1697, 1949, 1967
SspMI CTAG 5 cut(s) 38, 737, 1556, 2036, 2159
StyD4I CCNGG 1 cut(s) 121
StyI CCWWGG 4 cut(s) 460, 904, 1611, 2189
TaaI ACNGT 2 cut(s) 872, 1384
TaiI ACGT 4 cut(s) 273, 414, 717, 2316
TaqI TCGA 7 cut(s) 288, 563, 663, 689, 828, 1536, 1790
TaqII GACCGA 2 cut(s) 716, 2034
TatI WGTACW 2 cut(s) 1016, 1386
TauI GCSGC 3 cut(s) 1053, 1700, 1969
TfiI GAWTC 7 cut(s) 338, 530, 691, 883, 1134, 1616, 2279
Tru1I TTAA 7 cut(s) 20, 513, 921, 972, 1515, 2076, 2328
Tru9I TTAA 7 cut(s) 20, 513, 921, 972, 1515, 2076, 2328
TscAI CASTG 2 cut(s) 1389, 1557
TseFI GTSAC 3 cut(s) 271, 503, 2050
TseI GCWGC 8 cut(s) 41, 44, 298, 645, 765, 855, 2026, 2071
Tsp45I GTSAC 3 cut(s) 271, 503, 2050
TspGWI ACGGA 5 cut(s) 75, 368, 593, 1098, 1791
TspRI CASTG 2 cut(s) 1389, 1557
Van91I CCANNNNNTGG 2 cut(s) 251, 2330
VspI ATTAAT 3 cut(s) 513, 921, 972
XapI RAATTY 3 cut(s) 1395, 1981, 2204
XbaI TCTAGA 2 cut(s) 736, 2035
XceI RCATGY 4 cut(s) 765, 807, 825, 1870
XcmI CCANNNNNNNNNTGG 2 cut(s) 137, 1632
XhoI CTCGAG 1 cut(s) 1789
XmiI GTMKAC 4 cut(s) 288, 1080, 1404, 1733
XmnI GAANNNNTTC 2 cut(s) 723, 1226
XspI CTAG 5 cut(s) 38, 737, 1556, 2036, 2159
ZraI GACGTC 1 cut(s) 271
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.