Rroxscaffold_7G00161350

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000066
Physical Location & Seq
Forward (+)
4147511 .. 4150025
2515 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00161350.1

Sequence Viewer

Length: 2259 bp
ATGCTTCTCCAACTTCTGCTAATAAGGTCAGTACTAATAGCCACAGCAGCACAAGCACAACTAGCTGATCAATATGTGAAACCTGGTTGCGAAGCTACCTGTGGAAATGTTAACATACCATACCCTTTCGGTACCAGAGAAGCTTATTGTTACCATGATGAAGAGTTCCATGTAACTTGCAACTCCACATATGACCCTCCGAAACTATTTCTGTCCGGAGGCACTGTTGAGGTCACTAACATTTCTGTTGATGGCAAGTTGAATATCATGCAATATATAGCTCAAGACTGCTATGAATCATCCGGTTTCCGTATCAAGAACAATTCTCCATGGCTCCGCCTTTCAAAATTCATCATCTCCAACACCGATAATAAGTTCATTGCAGTTGGATGTGACACTTATGCAGAAATCCGAGGTTTCCAGGGCGAAATCAAGAAGTACACAGGTGGCTGCATATCAACGTGTGACAGCATTGATTATGTTGCCGGTGATTCATGTTCCGGTATAGGGTGTTGCCAGACATCAATAGCCAAAGGTGTGAGCTATTTTGATGTGTTTGTGGACAGTTATCGGAATCATACAGATGTTTGGTCTTTCAATCCATGCAGCTTCGCCTTTATTGTGGAAGAAAGCAAGTTCAACTTCTCCAGAGCTATGCTCATGGATTTAAAGAATGTAACAGTACTTCCTGTTGTGCTTGATTGGTCAATTGGAAATGAGACTTGCAAGCAAGTTGAAGGCAAAATGATAGCTGGCTACGCCGGAGGCAGCGCGTGCCAGGGGAACAGCAAATGTGTTGATGTCGAAAATGGGTCTGGATACCGATGCAAGTGCAAGGATGGTTATCAAGGGAGCCCATACCTCTCTAATGGTTGTCAAGATATTGATGAATGTGAGGATCCAAACCTCAATAACTGTGCACAAAGATGTGTAAATACAAAGGGAAGTCATCTCTGTCTTTGCCGCAAAGGGTACCATGGAAATGGTATAGTAGGGGAAGAAGGTTGCATAGCTAACAGAACATTAGTTGTTCAGATCACTGTTGGAATTGCTGTCGGCCTTATATCGTTGCTAATGTGTAGCTCTTGGTTATATCTGGGATACAGAAGATGGAAACTCATGAAGCTGAAGGAGAGATTCTTCAGACAAAATGGAGGATTAATGTTGCAACAACAACTTTCCGAACGACAAGGTGGTGCCACTAACCAAAATGCAAAAATCTTTACTGCAGAAGAACTTGAGAAGGCCACCAACAACTACCATGAGACTAGAATCGTTGGCAAAGGAGGCTACGGTACAGTGTACAAAGGAATTTTATCAGATGGAAGAGTAGTTGCAATCAAGAAATCCAAAATAGTTGATCAAAGCCAAATAGAGCAATTCATTAATGAAGTACTGGTGCTTTCCCAAATCAATCACAGGAATGTGGTGAAGCTTTTGGGTTGTTGTTTCGAGACAGAAGTCCCACGACTAGTTTATGAGTTTGTCACCAATGGTACGCTATTCGACTACATCCACAACAAAAGCAAGGCATCCAACTTTGCTTGGGAAACTCGTTTAAGGATAGCTGCAGAAGCTGCTGGAGTGCTATCATACTTGCACTCAGCTGCTTCTGTCCCTATTATCCATAGAGATGTCAAGTCCACAAATATACTTTTAGATGAAACCCTTACAGCAAAAGTGTCTGATTTTGGTGCCTCCAGATTGGTACCATTGGATCAAACTGAGTTATCGACAATGGTACAAGGCACACTCGGGTACTTAGACCCTGAATACTTTCAAACAAGCCAGTTGACTGACAAGAGTGATGTCTATAGCTTTGGGGTTGTCCTTGTGGAGCTGCTAACAGGAAAGAAGGCGCTTTCGTTCGACAGGCAAGAGGAGGATAGAAATCTAGCAATGTATTTTCTTTCCGCACTGAAAGAAAACCTCTTGGTACAAGTGGTTGATGAGTGTGTTCTGAATGGGGCAAATGACGAGCAGCTTAAGGAAGTTTCTAACCTTGCAAAGAGGTGTTTGAAAGTGAAAGGAGAAGAAAGACCAACCATGATGGAAGTAGCAAGCGAGCTTGAGGGACTAAGAAGGATGGCAATGCATCATCCCTGGGTTAATAATGATGAATCCAATGCACAGGAGAATGAAAGCTTGCTTGGTGAAATATCGCTGGAAACGTTTAGTCGTGGAGGTGGTGGCGATACGAGTTCTGGGTATGATACAATGAGGAACCACATCATATTACCAGTAAATGATGGGAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF_CA GUB_WAK_bind Pkinase WAK

Protein Analysis

752

Amino Acids

83.34

Weight (kDa)

5.57

Isoelectric Point (pI)

30.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 30 - 85 4.7e-11 Wall-associated receptor kinase galacturonan-binding
EGF_CA PF07645 294 - 328 2.9e-07 Calcium-binding EGF domain
Pkinase PF00069 420 - 687 9.5e-49 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 423 - 690 4e-47 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21210 AT1G21230 AT1G21240 AT1G21240 AT1G21245 AT1G21250 AT1G21270 AT1G22720 AT3G25490
fragaria_vesca FvH4_2g35051 FvH4_2g35051 FvH4_2g35060 FvH4_3g10281 FvH4_3g10285 FvH4_3g10286 FvH4_3g10311 FvH4_3g10311 FvH4_3g10320 FvH4_3g10332 FvH4_3g10340 FvH4_3g10350 FvH4_3g10360 FvH4_3g10390 FvH4_3g10412 FvH4_3g10413 FvH4_3g10461 FvH4_3g10462 FvH4_3g10470 FvH4_3g10510 FvH4_3g10520 FvH4_3g10530 FvH4_3g10550 FvH4_3g10560 FvH4_3g10611 FvH4_3g11730 FvH4_6g53140 FvH4_6g53142 FvH4_7g31880 FvH4_7g31910 FvH4_7g31920
malus_domestica MD05G1269900.v1.1 MD05G1270000.v1.1 MD05G1270200.v1.1 MD05G1270400.v1.1 MD05G1270500.v1.1 MD05G1270600.v1.1 MD05G1270800.v1.1 MD05G1270900.v1.1 MD07G1183800.v1.1 MD08G1107600.v1.1 MD10G1248500.v1.1 MD10G1249200.v1.1 MD10G1249500.v1.1 MD10G1250000.v1.1 MD10G1250500.v1.1 MD10G1250900.v1.1 MD10G1251200.v1.1 MD10G1251400.v1.1 MD13G1004600.v1.1 MD15G1088400.v1.1
prunus_persica Prupe.1G442400_v2.0.a1 Prupe.1G442500_v2.0.a1 Prupe.2G223400_v2.0.a1 Prupe.4G091900_v2.0.a1 Prupe.4G093200_v2.0.a1 Prupe.4G093300_v2.0.a1 Prupe.4G093400_v2.0.a1 Prupe.4G093500_v2.0.a1 Prupe.4G094100_v2.0.a1 Prupe.4G094200_v2.0.a1 Prupe.4G094300_v2.0.a1 Prupe.6G358300_v2.0.a1
pyrus_communis pycom08g08990 pycom10g20660 pycom10g20670 pycom10g20680 pycom10g20710 pycom10g20740 pycom13g00400 pycom15g08290
rosa_chinensis RchiOBHm_Chr1g0326861 RchiOBHm_Chr1g0326871 RchiOBHm_Chr1g0331391 RchiOBHm_Chr1g0336911 RchiOBHm_Chr1g0364001 RchiOBHm_Chr2g0174541 RchiOBHm_Chr2g0175041 RchiOBHm_Chr2g0175101 RchiOBHm_Chr2g0175121 RchiOBHm_Chr3g0486331 RchiOBHm_Chr5g0015581 RchiOBHm_Chr5g0016551 RchiOBHm_Chr5g0016561 RchiOBHm_Chr5g0016581 RchiOBHm_Chr5g0016601 RchiOBHm_Chr5g0016611 RchiOBHm_Chr5g0016641 RchiOBHm_Chr5g0016661 RchiOBHm_Chr5g0016691 RchiOBHm_Chr5g0016701 RchiOBHm_Chr5g0016731 RchiOBHm_Chr5g0016741 RchiOBHm_Chr5g0016811 RchiOBHm_Chr5g0016841 RchiOBHm_Chr5g0016871 RchiOBHm_Chr5g0016881 RchiOBHm_Chr5g0016931 RchiOBHm_Chr5g0017021 RchiOBHm_Chr5g0017041 RchiOBHm_Chr5g0017071 RchiOBHm_Chr5g0017091 RchiOBHm_Chr5g0017121 RchiOBHm_Chr5g0017141 RchiOBHm_Chr5g0017291 RchiOBHm_Chr5g0017321 RchiOBHm_Chr5g0017401 RchiOBHm_Chr5g0019001 RchiOBHm_Chr5g0019071 RchiOBHm_Chr5g0028941 RchiOBHm_Chr5g0052141 RchiOBHm_Chr5g0054721 RchiOBHm_Chr5g0054801 RchiOBHm_Chr5g0054931 RchiOBHm_Chr5g0054941 RchiOBHm_Chr6g0259291 RchiOBHm_Chr6g0292991 RchiOBHm_Chr6g0306911
rosa_laevigata RLG00000009465 RLG00000009468 RLG00000010755 RLG00000011976 RLG00000014541 RLG00000014542 RLG00000015169 RLG00000022308 RLG00000027529 RLG00000029324 RLG00000030244 RLG00000030245 RLG00000032210 RLG00000032211 RLG00000032257 RLG00000032259 RLG00000032260 RLG00000032261 RLG00000032262 RLG00000032268 RLG00000032269 RLG00000032272 RLG00000032282 RLG00000032283 RLG00000032308 RLG00000032413 RLG00000033129 RLG00000034945
rosa_multiflora Rmu_co8241929.1_g000001 Rmu_co8269227.1_g000001 Rmu_co8461259.1_g000001 Rmu_co8495385.1_g000001 Rmu_sc0000028.1_g000003 Rmu_sc0000083.1_g000009 Rmu_sc0000083.1_g000010 Rmu_sc0000083.1_g000031 Rmu_sc0000083.1_g000043 Rmu_sc0000083.1_g000046 Rmu_sc0000083.1_g000047 Rmu_sc0000563.1_g000024 Rmu_sc0000842.1_g000012 Rmu_sc0001235.1_g000001 Rmu_sc0001235.1_g000005 Rmu_sc0001235.1_g000009 Rmu_sc0001235.1_g000015 Rmu_sc0001235.1_g000016 Rmu_sc0001235.1_g000017 Rmu_sc0001473.1_g000036 Rmu_sc0001607.1_g000016 Rmu_sc0001788.1_g000006 Rmu_sc0002018.1_g000008 Rmu_sc0002028.1_g000001 Rmu_sc0002680.1_g000011 Rmu_sc0002798.1_g000023 Rmu_sc0002886.1_g000023 Rmu_sc0002989.1_g000012 Rmu_sc0003064.1_g000009 Rmu_sc0003382.1_g000006 Rmu_sc0003630.1_g000002 Rmu_sc0003630.1_g000047 Rmu_sc0003630.1_g000066 Rmu_sc0003630.1_g000067 Rmu_sc0003649.1_g000010 Rmu_sc0003688.1_g000005 Rmu_sc0003920.1_g000007 Rmu_sc0004765.1_g000007 Rmu_sc0004765.1_g000008 Rmu_sc0004920.1_g000011 Rmu_sc0005223.1_g000008 Rmu_sc0005223.1_g000010 Rmu_sc0005223.1_g000015 Rmu_sc0005223.1_g000016 Rmu_sc0005319.1_g000014 Rmu_sc0005319.1_g000015 Rmu_sc0005319.1_g000018 Rmu_sc0005319.1_g000019 Rmu_sc0005319.1_g000025 Rmu_sc0005418.1_g000007 Rmu_sc0005418.1_g000012 Rmu_sc0006106.1_g000011 Rmu_sc0006914.1_g000012 Rmu_sc0007023.1_g000001 Rmu_sc0007662.1_g000001 Rmu_sc0007920.1_g000002 Rmu_sc0007920.1_g000013 Rmu_sc0008053.1_g000016 Rmu_sc0008053.1_g000017 Rmu_sc0008053.1_g000018 Rmu_sc0008053.1_g000023 Rmu_sc0008053.1_g000050 Rmu_sc0009042.1_g000007 Rmu_sc0009042.1_g000008 Rmu_sc0010642.1_g000008 Rmu_sc0011109.1_g000002 Rmu_sc0011314.1_g000006 Rmu_sc0011974.1_g000006 Rmu_sc0012179.1_g000009 Rmu_sc0015169.1_g000001 Rmu_sc0016368.1_g000002 Rmu_sc0022659.1_g000001 Rmu_sc0027371.1_g000001 Rmu_sc0028213.1_g000001 Rmu_sc0034526.1_g000001 Rmu_sc0039366.1_g000002 Rmu_ssc0000050.1_g000084 Rmu_ssc0000050.1_g000088 Rmu_ssc0000167.1_g000017
rosa_roxburghii Rroxscaffold_1G00011340 Rroxscaffold_1G00025710 Rroxscaffold_1G00025870 Rroxscaffold_1G00026070 Rroxscaffold_1G00026170 Rroxscaffold_1G00026200 Rroxscaffold_1G00026270 Rroxscaffold_1G00050950 Rroxscaffold_1G00058900 Rroxscaffold_1G00060040 Rroxscaffold_1G00060060 Rroxscaffold_1G00060070 Rroxscaffold_1G00060080 Rroxscaffold_1G00060110 Rroxscaffold_1G00060120 Rroxscaffold_1G00060140 Rroxscaffold_1G00060170 Rroxscaffold_1G00060210 Rroxscaffold_1G00060220 Rroxscaffold_1G00060240 Rroxscaffold_1G00060250 Rroxscaffold_1G00060260 Rroxscaffold_1G00060300 Rroxscaffold_1G00060320 Rroxscaffold_1G00060330 Rroxscaffold_1G00060900 Rroxscaffold_1G00060920 Rroxscaffold_1G00073980 Rroxscaffold_2G00077560 Rroxscaffold_2G00077580 Rroxscaffold_2G00146990 Rroxscaffold_3G00236200 Rroxscaffold_3G00236210 Rroxscaffold_3G00236220 Rroxscaffold_4G00292760 Rroxscaffold_4G00315200 Rroxscaffold_4G00315240 Rroxscaffold_4G00326240 Rroxscaffold_4G00328980 Rroxscaffold_5G00341890 Rroxscaffold_7G00161350 Rroxscaffold_7G00174490 Rroxscaffold_7G00206550
rosa_rugosa Rorug01G0093000 Rorug01G0093100 Rorug01G0128800 Rorug01G0312000 Rorug01G0312000 Rorug02G0581100 Rorug03G0224600 Rorug05G0028500 Rorug05G0032700 Rorug05G0032800 Rorug05G0032900 Rorug05G0032900 Rorug05G0033000 Rorug05G0033100 Rorug05G0033300 Rorug05G0033400 Rorug05G0033900 Rorug05G0034900 Rorug05G0035000 Rorug05G0046900 Rorug05G0047500 Rorug05G0047500 Rorug05G0111600 Rorug05G0287900 Rorug05G0288700 Rorug05G0586900 Rorug06G0231700 Rorug06G0354300 Rorug06G0354400
rosa_samantha Rh1DG122200 Rh2DG686800 Rh5BG136600 Rh6AG344500 Rh6BG351900 Rh6CG359000 Rh6CG359100 Rh6CG387600 Rh6CG480500 Rh6DG344600 Rh6DG467400
rosa_wichuraiana Rw0G010250 Rw0G014820 Rw0G021400 Rw0G023100 Rw1G009450 Rw1G012100 Rw1G012180 Rw1G028360 Rw2G054260 Rw3G024370 Rw3G024420 Rw3G024470 Rw5G010850 Rw5G011010 Rw5G011020 Rw5G011050 Rw5G011080 Rw5G011120 Rw5G012300 Rw5G018470 Rw5G033830 Rw6G008930 Rw6G030070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 3 cut(s) 131, 972, 1708
AccB1I GGYRCC 5 cut(s) 131, 972, 1196, 1694, 1708
AccII CGCG 1 cut(s) 773
AccIII TCCGGA 1 cut(s) 215
AciI CCGC 3 cut(s) 337, 964, 1914
AclI AACGTT 1 cut(s) 2171
AclWI GGATC 3 cut(s) 893, 906, 1725
AcsI RAATTY 2 cut(s) 347, 1311
AcuI CTGAAG 2 cut(s) 1126, 1148
AfiI CCNNNNNNNGG 1 cut(s) 507
AflII CTTAAG 1 cut(s) 1985
AflIII ACRYGT 1 cut(s) 461
AgsI TTSAA 7 cut(s) 262, 345, 598, 640, 737, 1781, 2020
AhlI ACTAGT 1 cut(s) 1471
AjnI CCWGG 4 cut(s) 82, 420, 777, 2102
AjuI GAANNNNNNNTTGG 2 cut(s) 2133, 2165
Alw21I GWGCWC 1 cut(s) 922
Alw26I GTCTC 3 cut(s) 713, 1259, 1448
Alw44I GTGCAC 1 cut(s) 918
AlwI GGATC 3 cut(s) 893, 906, 1725
AlwNI CAGNNNCTG 1 cut(s) 1577
Ama87I CYCGRG 1 cut(s) 1754
Aor13HI TCCGGA 1 cut(s) 215
AoxI GGCC 2 cut(s) 1057, 1245
ApaLI GTGCAC 1 cut(s) 918
ApeKI GCWGC 9 cut(s) 47, 450, 606, 768, 1568, 1577, 1607, 1840, 1981
ApoI RAATTY 2 cut(s) 347, 1311
AseI ATTAAT 2 cut(s) 1160, 1386
Asp700I GAANNNNTTC 1 cut(s) 1776
Asp718I GGTACC 3 cut(s) 131, 972, 1708
AspLEI GCGC 2 cut(s) 773, 1861
AsuHPI GGTGA 4 cut(s) 500, 1441, 1480, 2165
AvaI CYCGRG 1 cut(s) 1754
BaeGI GKGCMC 1 cut(s) 922
BamHI GGATCC 1 cut(s) 898
BanI GGYRCC 5 cut(s) 131, 972, 1196, 1694, 1708
BanII GRGCYC 1 cut(s) 857
BarI GAAGNNNNNNTAC 2 cut(s) 669, 701
Bbv12I GWGCWC 1 cut(s) 922
BbvI GCAGC 9 cut(s) 59, 437, 618, 780, 1555, 1564, 1594, 1827, 1993
BccI CCATC 7 cut(s) 245, 833, 1104, 1316, 2044, 2080, 2243
BcgI CGANNNNNNTGC 2 cut(s) 813, 847
BciT130I CCWGG 4 cut(s) 84, 422, 779, 2104
BciVI GTATCC 2 cut(s) 812, 1094
BclI TGATCA 2 cut(s) 67, 1360
BcoDI GTCTC 3 cut(s) 713, 1259, 1448
BcuI ACTAGT 1 cut(s) 1471
BfaI CTAG 4 cut(s) 62, 1269, 1472, 1895
BfmI CTRYAG 3 cut(s) 1227, 1569, 1813
BfoI RGCGCY 1 cut(s) 1862
BfrI CTTAAG 1 cut(s) 1985
BfuI GTATCC 2 cut(s) 812, 1094
BmcAI AGTACT 3 cut(s) 33, 684, 1395
Bme1390I CCNGG 4 cut(s) 84, 422, 779, 2104
BmeT110I CYCGRG 1 cut(s) 1754
BmiI GGNNCC 9 cut(s) 133, 335, 854, 900, 974, 1198, 1696, 1710, 2225
BmrFI CCNGG 4 cut(s) 84, 422, 779, 2104
BmsI GCATC 3 cut(s) 815, 1541, 2104
BoxI GACNNNNGTC 1 cut(s) 1460
BplI GAGNNNNNCTC 2 cut(s) 642, 674
BpmI CTGGAG 3 cut(s) 631, 1602, 1684
BpuEI CTTGAG 3 cut(s) 267, 1259, 2090
BsaBI GATNNNNATC 2 cut(s) 843, 1890
BsaJI CCNNGG 7 cut(s) 329, 412, 421, 778, 976, 2102, 2103
BsaWI WCCGGW 3 cut(s) 215, 302, 500
BsaXI ACNNNNNCTCC 2 cut(s) 210, 240
Bsc4I CCNNNNNNNGG 1 cut(s) 507
Bse118I RCCGGY 1 cut(s) 485
Bse1I ACTGG 3 cut(s) 1401, 1789, 2240
Bse3DI GCAATG 3 cut(s) 378, 1905, 2097
Bse8I GATNNNNATC 2 cut(s) 843, 1890
BseAI TCCGGA 1 cut(s) 215
BseBI CCWGG 4 cut(s) 84, 422, 779, 2104
BseDI CCNNGG 7 cut(s) 329, 412, 421, 778, 976, 2102, 2103
BseGI GGATG 7 cut(s) 299, 395, 844, 1512, 1532, 2091, 2098
BseJI GATNNNNATC 2 cut(s) 843, 1890
BseLI CCNNNNNNNGG 1 cut(s) 507
BseMI GCAATG 3 cut(s) 378, 1905, 2097
BseMII CTCAG 2 cut(s) 1617, 1716
BseNI ACTGG 3 cut(s) 1401, 1789, 2240
BseRI GAGGAG 1 cut(s) 1895
BseSI GKGCMC 1 cut(s) 922
BseXI GCAGC 9 cut(s) 59, 437, 618, 780, 1555, 1564, 1594, 1827, 1993
Bsh1236I CGCG 1 cut(s) 773
BshFI GGCC 2 cut(s) 1059, 1247
BshNI GGYRCC 5 cut(s) 131, 972, 1196, 1694, 1708
BsiHKAI GWGCWC 1 cut(s) 922
BsiHKCI CYCGRG 1 cut(s) 1754
BsiSI CCGG 5 cut(s) 216, 303, 486, 501, 762
BslFI GGGAC 3 cut(s) 1448, 1601, 2088
BslI CCNNNNNNNGG 1 cut(s) 507
BsmAI GTCTC 3 cut(s) 713, 1259, 1448
BsmFI GGGAC 3 cut(s) 1448, 1601, 2088
BsnI GGCC 2 cut(s) 1059, 1247
BsoBI CYCGRG 1 cut(s) 1754
Bsp1286I GDGCHC 2 cut(s) 857, 922
Bsp13I TCCGGA 1 cut(s) 215
Bsp1407I TGTACA 1 cut(s) 1302
Bsp143I GATC 5 cut(s) 67, 898, 1035, 1360, 1717
Bsp19I CCATGG 2 cut(s) 329, 976
BspACI CCGC 3 cut(s) 337, 964, 1914
BspANI GGCC 2 cut(s) 1059, 1247
BspCNI CTCAG 2 cut(s) 1616, 1717
BspEI TCCGGA 1 cut(s) 215
BspFNI CGCG 1 cut(s) 773
BspHI TCATGA 1 cut(s) 1119
BspLI GGNNCC 9 cut(s) 133, 335, 854, 900, 974, 1198, 1696, 1710, 2225
BspMAI CTGCAG 2 cut(s) 1231, 1573
BspPI GGATC 3 cut(s) 893, 906, 1725
BspT107I GGYRCC 5 cut(s) 131, 972, 1196, 1694, 1708
BspTI CTTAAG 1 cut(s) 1985
BsrDI GCAATG 3 cut(s) 378, 1905, 2097
BsrFI RCCGGY 1 cut(s) 485
BsrGI TGTACA 1 cut(s) 1302
BsrI ACTGG 3 cut(s) 1401, 1789, 2240
BssAI RCCGGY 1 cut(s) 485
BssECI CCNNGG 7 cut(s) 329, 412, 421, 778, 976, 2102, 2103
BssMI GATC 5 cut(s) 67, 898, 1035, 1360, 1717
BssT1I CCWWGG 2 cut(s) 329, 976
Bst2UI CCWGG 4 cut(s) 84, 422, 779, 2104
Bst4CI ACNGT 7 cut(s) 226, 566, 682, 917, 1042, 1295, 1300
Bst6I CTCTTC 2 cut(s) 156, 1321
BstAFI CTTAAG 1 cut(s) 1985
BstAPI GCANNNNNTGC 2 cut(s) 774, 1577
BstAUI TGTACA 1 cut(s) 1302
BstC8I GCNNGC 6 cut(s) 728, 754, 775, 2062, 2066, 2147
BstDEI CTNAG 4 cut(s) 1603, 1725, 1762, 2078
BstDSI CCRYGG 2 cut(s) 329, 976
BstF5I GGATG 7 cut(s) 299, 395, 844, 1512, 1532, 2091, 2098
BstFNI CGCG 1 cut(s) 773
BstH2I RGCGCY 1 cut(s) 1862
BstHHI GCGC 2 cut(s) 773, 1861
BstKTI GATC 5 cut(s) 70, 901, 1038, 1363, 1720
BstMAI GTCTC 3 cut(s) 713, 1259, 1448
BstMBI GATC 5 cut(s) 67, 898, 1035, 1360, 1717
BstMWI GCNNNNNNNGC 8 cut(s) 47, 53, 62, 758, 774, 1287, 1574, 1577
BstNI CCWGG 4 cut(s) 84, 422, 779, 2104
BstPAI GACNNNNGTC 1 cut(s) 1460
BstSCI CCNGG 4 cut(s) 82, 420, 777, 2102
BstSFI CTRYAG 3 cut(s) 1227, 1569, 1813
BstSLI GKGCMC 1 cut(s) 922
BstUI CGCG 1 cut(s) 773
BstV1I GCAGC 9 cut(s) 59, 437, 618, 780, 1555, 1564, 1594, 1827, 1993
BstX2I RGATCY 1 cut(s) 898
BstXI CCANNNNNNTGG 1 cut(s) 983
BstYI RGATCY 1 cut(s) 898
BsuI GTATCC 2 cut(s) 812, 1094
BsuRI GGCC 2 cut(s) 1059, 1247
BtgI CCRYGG 2 cut(s) 329, 976
BtsCI GGATG 7 cut(s) 299, 395, 844, 1512, 1532, 2091, 2098
BtsIMutI CAGTG 4 cut(s) 222, 1038, 1305, 1916
Cac8I GCNNGC 6 cut(s) 728, 754, 775, 2062, 2066, 2147
CaiI CAGNNNCTG 1 cut(s) 1577
CciI TCATGA 1 cut(s) 1119
CfoI GCGC 2 cut(s) 773, 1861
Cfr10I RCCGGY 1 cut(s) 485
CsiI ACCWGGT 1 cut(s) 82
DdeI CTNAG 4 cut(s) 1603, 1725, 1762, 2078
DpnI GATC 5 cut(s) 69, 900, 1037, 1362, 1719
DpnII GATC 5 cut(s) 67, 898, 1035, 1360, 1717
DraI TTTAAA 1 cut(s) 669
Eam1104I CTCTTC 2 cut(s) 156, 1321
EarI CTCTTC 2 cut(s) 156, 1321
EciI GGCGGA 1 cut(s) 326
Eco130I CCWWGG 2 cut(s) 329, 976
Eco24I GRGCYC 1 cut(s) 857
Eco57I CTGAAG 2 cut(s) 1126, 1148
Eco88I CYCGRG 1 cut(s) 1754
EcoRII CCWGG 4 cut(s) 82, 420, 777, 2102
EcoT14I CCWWGG 2 cut(s) 329, 976
EcoT22I ATGCAT 1 cut(s) 2097
EcoT38I GRGCYC 1 cut(s) 857
ErhI CCWWGG 2 cut(s) 329, 976
FalI AAGNNNNNCTT 2 cut(s) 626, 658
FaqI GGGAC 3 cut(s) 1448, 1601, 2088
FauNDI CATATG 1 cut(s) 190
FbaI TGATCA 2 cut(s) 67, 1360
FokI GGATG 7 cut(s) 286, 402, 851, 1499, 1519, 2085, 2098
FriOI GRGCYC 1 cut(s) 857
FspBI CTAG 4 cut(s) 62, 1269, 1472, 1895
GlaI GCGC 2 cut(s) 772, 1860
GsuI CTGGAG 3 cut(s) 631, 1602, 1684
HaeII RGCGCY 1 cut(s) 1862
HaeIII GGCC 2 cut(s) 1059, 1247
HapII CCGG 5 cut(s) 216, 303, 486, 501, 762
HhaI GCGC 2 cut(s) 773, 1861
Hin6I GCGC 2 cut(s) 771, 1859
HinP1I GCGC 2 cut(s) 771, 1859
HincII GTYRAC 2 cut(s) 112, 1794
HindII GTYRAC 2 cut(s) 112, 1794
HindIII AAGCTT 3 cut(s) 141, 1433, 2143
HinfI GANTC 6 cut(s) 296, 491, 574, 1137, 1272, 2120
HpaI GTTAAC 1 cut(s) 112
HpaII CCGG 5 cut(s) 216, 303, 486, 501, 762
HphI GGTGA 4 cut(s) 500, 1441, 1480, 2165
Hpy166II GTNNAC 7 cut(s) 112, 441, 562, 920, 1303, 1644, 1794
Hpy188I TCNGA 9 cut(s) 201, 413, 573, 1035, 1145, 1183, 1321, 1687, 1962
Hpy8I GTNNAC 7 cut(s) 112, 441, 562, 920, 1303, 1644, 1794
HpyAV CCTTC 6 cut(s) 731, 995, 1123, 1237, 1849, 2076
HpyCH4III ACNGT 7 cut(s) 226, 566, 682, 917, 1042, 1295, 1300
HpyCH4IV ACGT 2 cut(s) 461, 2171
HpyF10VI GCNNNNNNNGC 8 cut(s) 47, 53, 62, 758, 774, 1287, 1574, 1577
HpyF3I CTNAG 4 cut(s) 1603, 1725, 1762, 2078
HpySE526I ACGT 2 cut(s) 461, 2171
HspAI GCGC 2 cut(s) 771, 1859
Kpn2I TCCGGA 1 cut(s) 215
KpnI GGTACC 3 cut(s) 135, 976, 1712
Ksp22I TGATCA 2 cut(s) 67, 1360
KspAI GTTAAC 1 cut(s) 112
Kzo9I GATC 5 cut(s) 67, 898, 1035, 1360, 1717
LmnI GCTCC 3 cut(s) 339, 852, 1837
Lsp1109I GCAGC 9 cut(s) 59, 437, 618, 780, 1555, 1564, 1594, 1827, 1993
LweI GCATC 3 cut(s) 815, 1541, 2104
MabI ACCWGGT 1 cut(s) 82
MaeI CTAG 4 cut(s) 62, 1269, 1472, 1895
MaeII ACGT 2 cut(s) 461, 2171
MaeIII GTNAC 7 cut(s) 149, 172, 232, 392, 464, 676, 1486
MalI GATC 5 cut(s) 69, 900, 1037, 1362, 1719
MboI GATC 5 cut(s) 67, 898, 1035, 1360, 1717
MboII GAAGA 8 cut(s) 173, 638, 1010, 1119, 1132, 1244, 1338, 2045
MfeI CAATTG 1 cut(s) 708
MflI RGATCY 1 cut(s) 898
MhlI GDGCHC 2 cut(s) 857, 922
MluCI AATT 6 cut(s) 322, 347, 708, 1047, 1311, 1379
MmeI TCCRAC 5 cut(s) 34, 367, 384, 1024, 1560
Mph1103I ATGCAT 1 cut(s) 2097
MroI TCCGGA 1 cut(s) 215
MroXI GAANNNNTTC 1 cut(s) 1776
MseI TTAA 7 cut(s) 111, 668, 1160, 1386, 1559, 1986, 2109
MslI CAYNNNNRTG 5 cut(s) 582, 925, 981, 1422, 1632
MspA1I CMGCKG 1 cut(s) 1607
MspCI CTTAAG 1 cut(s) 1985
MspI CCGG 5 cut(s) 216, 303, 486, 501, 762
MspR9I CCNGG 4 cut(s) 84, 422, 779, 2104
MunI CAATTG 1 cut(s) 708
MvaI CCWGG 4 cut(s) 84, 422, 779, 2104
MvnI CGCG 1 cut(s) 773
MwoI GCNNNNNNNGC 8 cut(s) 47, 53, 62, 758, 774, 1287, 1574, 1577
NcoI CCATGG 2 cut(s) 329, 976
NdeI CATATG 1 cut(s) 190
NdeII GATC 5 cut(s) 67, 898, 1035, 1360, 1717
NlaIV GGNNCC 9 cut(s) 133, 335, 854, 900, 974, 1198, 1696, 1710, 2225
NmuCI GTSAC 4 cut(s) 232, 392, 464, 1486
NsiI ATGCAT 1 cut(s) 2097
PagI TCATGA 1 cut(s) 1119
PasI CCCWGGG 1 cut(s) 2103
PcsI WCGNNNNNNNCGW 1 cut(s) 2168
PdmI GAANNNNTTC 1 cut(s) 1776
PfeI GAWTC 6 cut(s) 296, 491, 574, 1137, 1272, 2120
PshAI GACNNNNGTC 1 cut(s) 1460
PshBI ATTAAT 2 cut(s) 1160, 1386
Psp1406I AACGTT 1 cut(s) 2171
Psp6I CCWGG 4 cut(s) 82, 420, 777, 2102
PspGI CCWGG 4 cut(s) 82, 420, 777, 2102
PspN4I GGNNCC 9 cut(s) 133, 335, 854, 900, 974, 1198, 1696, 1710, 2225
PstI CTGCAG 2 cut(s) 1231, 1573
PstNI CAGNNNCTG 1 cut(s) 1577
PsuI RGATCY 1 cut(s) 898
PvuII CAGCTG 1 cut(s) 1607
RseI CAYNNNNRTG 5 cut(s) 582, 925, 981, 1422, 1632
SaqAI TTAA 7 cut(s) 111, 668, 1160, 1386, 1559, 1986, 2109
Sau3AI GATC 5 cut(s) 67, 898, 1035, 1360, 1717
ScaI AGTACT 3 cut(s) 33, 684, 1395
ScrFI CCNGG 4 cut(s) 84, 422, 779, 2104
SduI GDGCHC 2 cut(s) 857, 922
SexAI ACCWGGT 1 cut(s) 82
SfaNI GCATC 3 cut(s) 815, 1541, 2104
SfcI CTRYAG 3 cut(s) 1227, 1569, 1813
SmiMI CAYNNNNRTG 5 cut(s) 582, 925, 981, 1422, 1632
SmlI CTYRAG 4 cut(s) 282, 1238, 1985, 2069
SmoI CTYRAG 4 cut(s) 282, 1238, 1985, 2069
SpeI ACTAGT 1 cut(s) 1471
Sse9I AATT 6 cut(s) 322, 347, 708, 1047, 1311, 1379
SsiI CCGC 3 cut(s) 337, 964, 1914
SspMI CTAG 4 cut(s) 62, 1269, 1472, 1895
StyD4I CCNGG 4 cut(s) 82, 420, 777, 2102
StyI CCWWGG 2 cut(s) 329, 976
TaaI ACNGT 7 cut(s) 226, 566, 682, 917, 1042, 1295, 1300
TaiI ACGT 2 cut(s) 464, 2174
TaqI TCGA 5 cut(s) 804, 1452, 1506, 1733, 1869
TasI AATT 6 cut(s) 322, 347, 708, 1047, 1311, 1379
TatI WGTACW 5 cut(s) 31, 438, 682, 1302, 1393
TauI GCSGC 1 cut(s) 966
TfiI GAWTC 6 cut(s) 296, 491, 574, 1137, 1272, 2120
Tru1I TTAA 7 cut(s) 111, 668, 1160, 1386, 1559, 1986, 2109
Tru9I TTAA 7 cut(s) 111, 668, 1160, 1386, 1559, 1986, 2109
TscAI CASTG 4 cut(s) 229, 1045, 1305, 1923
TseFI GTSAC 4 cut(s) 232, 392, 464, 1486
TseI GCWGC 9 cut(s) 47, 450, 606, 768, 1568, 1577, 1607, 1840, 1981
Tsp45I GTSAC 4 cut(s) 232, 392, 464, 1486
TspGWI ACGGA 1 cut(s) 299
TspRI CASTG 4 cut(s) 229, 1045, 1305, 1923
Vha464I CTTAAG 1 cut(s) 1985
VneI GTGCAC 1 cut(s) 918
VspI ATTAAT 2 cut(s) 1160, 1386
XapI RAATTY 2 cut(s) 347, 1311
XmnI GAANNNNTTC 1 cut(s) 1776
XspI CTAG 4 cut(s) 62, 1269, 1472, 1895
ZrmI AGTACT 3 cut(s) 33, 684, 1395
Zsp2I ATGCAT 1 cut(s) 2097
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.