Rroxscaffold_1G00060210

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000066
Physical Location & Seq
Forward (+)
82427750 .. 82430673
2924 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00060210.1

Sequence Viewer

Length: 2094 bp
ATGCAATTCATACTGATAGCGGTACTGGTTCTCCTCACATTTGCGGCCGCGGCCGAAGAAGCAGTAGCAGCTGAAGCCGTCCTGCCTCAAGCCAAGCCTGGCTGCACTGACCTCTGTGGCAACCTCACCATTCCATATCCATTTGGCATAGGGCCTGATTGTTACATGACACAAGACTTTTCTGTTAATTGTAACACATCCACTGAACCCCCAACAGCAATGTGGGATGATAGTAACATCGCTATTGACAACATATACCTGGCTGAAGGTGAGATGCAAATAGCGCAGTACGTAGCCTACGATTGCTATGATAGCCAGGGCTGGACTAAATACAGCAAACCATCGCTCGCCGTGCCTCCTTTCATCATCTCTCATACTAGAAACAAGTTCATTGCCATTGGTTGCGATGCTTATGCACTTTTCAGAGGTTACCGTGCAGACGAAGAGAGTTACCTGACCGGATGCATGTCCATATGTGACAACCTTGACAGCGTTGAACAGTCTTGCTCCGGGGTTGGGTGTTGCCAAACTGACATCCCTAGAGGACTTAAGAACTACACTCTCAGACTGGATAGCTACTATAATCATACATACATACTGGACTTCAATCCATGCAGCTATGCCTTCATTGTGGAAGAAGGCAAGTTTACCTTTTCCCCGAGTACAAGTTTTGAAGAACTCGCCAACACTGAAAAGCTTCCCGCGATTCTTAATTGGGAAATTGGGAAAGGGCCATGTGATGAAGCTCAAAAGAAAGATGATTATGCATGCAAGGGAAATAGTAAGTGTGTCAATCGGAACATCAGCGATGAGCGATCTGGTTATCTTTGTCACTGCCAGCCGGGTTATCAAGGGAACCCCTACCTCCCTGATGGTTGCCAAGGTGTAACTGGCAGCTTCTTAGTTCTATTTGTTGGAATTTCATGGATATGTTGCGGAATAAAGAACAGAAAATTCATGAAACTCAAAGAAAAGTACTTTAAAGAGAATGGTGGCTTATTGTTACTACAGAAACTCGCTAATAATGGAGGCTCAGTGGAGACAACAAAAATCTTTTCCACAGAAGCACTTGAGAAGGCAACAAACAATTACCACGAGAGTAGAATCCTTGGAGAAGGAGGCTATGGAACAGTTTACAAAGGAATACTACCAGATGACAAAGTGGTGGCCATAAAGAAGTCAAAAGGTGGTGCCCCAACCCAAAGTGATCAGTTTGTTAACGAGGTGATTGTTCTTTCTCAAATAAACCACAGAAATGTGGTGAAGCTACTAGGTTGTTGTTTAGAGACAGCAGTACCTTTACTAGTATATGAGTTTGTCACCCATGGCACTTTATATGAGCACATTCATAGAAAAAGATCACTACTGTCATTTGAACTACGAATGAAGATAGCTGTTGAAACAGCAGGAGCACTAGCATACTTACACTCCTCTATATCTACGCCAATCATACATCGAGATGTGAAAGCAGCAAATATACTCCTAGATGATAATTATACAGCTAAAGTGTCGGACTTTGGAGCTTCACGGTTGGTTCCTTCAGGTCAAACTGAAATACAAACTTTAGTGCTCGGGACATTTGGATACCTAGACCCAGAATATTTGCAGTCAAACCAACTAACAGAAAAAAGTGATGTCTACAGCTTTGGAGTTGTCCTAGTGGAGCTACTAACAAGCAAAGTGGCATTTTCTAATGATAGATGCTTAGCAAGCATCTTTGTTTCTTCCATGGATGTAGATTGCTTAAAACAAATTCTTGATGATGCCATAGTGAATGAGGGAAACATTGAGACAGTAGAAAACGTGGCGAGTCTCGCAAAAAGATGTTTGAGGGTAAAAGGGGAGGAAAGGCCTACCATGAAAGAAGTTGCCATGGAGTTAGAGGGAATGGCAATCATGGCAAAGCATCCATGGGGAAAGAATGCCATGTTTTGTCCAGAGGAGAATGAGTACTTGCTTGGGTCACCTTCAGACAGTTATGTTATGGATGTTAGAGGAGGTGATCGCAATTCTAGTGGTTTAACTACTGGTATGACTACTGGGTATGACAGCATGCAAATCCAGATGTTGATGCCACGTGGTGATGGACGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK

Protein Analysis

697

Amino Acids

76.85

Weight (kDa)

5.07

Isoelectric Point (pI)

37.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 35 - 95 7.1e-10 Wall-associated receptor kinase galacturonan-binding
Pkinase PF00069 364 - 623 1.8e-50 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 367 - 627 4.4e-49 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21210 AT1G21230 AT1G21240 AT1G21240 AT1G21245 AT1G21250 AT1G21270 AT1G22720 AT3G25490
fragaria_vesca FvH4_2g35051 FvH4_2g35051 FvH4_2g35060 FvH4_3g10281 FvH4_3g10285 FvH4_3g10286 FvH4_3g10311 FvH4_3g10311 FvH4_3g10320 FvH4_3g10332 FvH4_3g10340 FvH4_3g10350 FvH4_3g10360 FvH4_3g10390 FvH4_3g10412 FvH4_3g10413 FvH4_3g10461 FvH4_3g10462 FvH4_3g10470 FvH4_3g10510 FvH4_3g10520 FvH4_3g10530 FvH4_3g10550 FvH4_3g10560 FvH4_3g10611 FvH4_3g11730 FvH4_6g53140 FvH4_6g53142 FvH4_7g31880 FvH4_7g31910 FvH4_7g31920
malus_domestica MD05G1269900.v1.1 MD05G1270000.v1.1 MD05G1270200.v1.1 MD05G1270400.v1.1 MD05G1270500.v1.1 MD05G1270600.v1.1 MD05G1270800.v1.1 MD05G1270900.v1.1 MD07G1183800.v1.1 MD08G1107600.v1.1 MD10G1248500.v1.1 MD10G1249200.v1.1 MD10G1249500.v1.1 MD10G1250000.v1.1 MD10G1250500.v1.1 MD10G1250900.v1.1 MD10G1251200.v1.1 MD10G1251400.v1.1 MD13G1004600.v1.1 MD15G1088400.v1.1
prunus_persica Prupe.1G442400_v2.0.a1 Prupe.1G442500_v2.0.a1 Prupe.2G223400_v2.0.a1 Prupe.4G091900_v2.0.a1 Prupe.4G093200_v2.0.a1 Prupe.4G093300_v2.0.a1 Prupe.4G093400_v2.0.a1 Prupe.4G093500_v2.0.a1 Prupe.4G094100_v2.0.a1 Prupe.4G094200_v2.0.a1 Prupe.4G094300_v2.0.a1 Prupe.6G358300_v2.0.a1
pyrus_communis pycom08g08990 pycom10g20660 pycom10g20670 pycom10g20680 pycom10g20710 pycom10g20740 pycom13g00400 pycom15g08290
rosa_chinensis RchiOBHm_Chr1g0326861 RchiOBHm_Chr1g0326871 RchiOBHm_Chr1g0331391 RchiOBHm_Chr1g0336911 RchiOBHm_Chr1g0364001 RchiOBHm_Chr2g0174541 RchiOBHm_Chr2g0175041 RchiOBHm_Chr2g0175101 RchiOBHm_Chr2g0175121 RchiOBHm_Chr3g0486331 RchiOBHm_Chr5g0015581 RchiOBHm_Chr5g0016551 RchiOBHm_Chr5g0016561 RchiOBHm_Chr5g0016581 RchiOBHm_Chr5g0016601 RchiOBHm_Chr5g0016611 RchiOBHm_Chr5g0016641 RchiOBHm_Chr5g0016661 RchiOBHm_Chr5g0016691 RchiOBHm_Chr5g0016701 RchiOBHm_Chr5g0016731 RchiOBHm_Chr5g0016741 RchiOBHm_Chr5g0016811 RchiOBHm_Chr5g0016841 RchiOBHm_Chr5g0016871 RchiOBHm_Chr5g0016881 RchiOBHm_Chr5g0016931 RchiOBHm_Chr5g0017021 RchiOBHm_Chr5g0017041 RchiOBHm_Chr5g0017071 RchiOBHm_Chr5g0017091 RchiOBHm_Chr5g0017121 RchiOBHm_Chr5g0017141 RchiOBHm_Chr5g0017291 RchiOBHm_Chr5g0017321 RchiOBHm_Chr5g0017401 RchiOBHm_Chr5g0019001 RchiOBHm_Chr5g0019071 RchiOBHm_Chr5g0028941 RchiOBHm_Chr5g0052141 RchiOBHm_Chr5g0054721 RchiOBHm_Chr5g0054801 RchiOBHm_Chr5g0054931 RchiOBHm_Chr5g0054941 RchiOBHm_Chr6g0259291 RchiOBHm_Chr6g0292991 RchiOBHm_Chr6g0306911
rosa_laevigata RLG00000009465 RLG00000009468 RLG00000010755 RLG00000011976 RLG00000014541 RLG00000014542 RLG00000015169 RLG00000022308 RLG00000027529 RLG00000029324 RLG00000030244 RLG00000030245 RLG00000032210 RLG00000032211 RLG00000032257 RLG00000032259 RLG00000032260 RLG00000032261 RLG00000032262 RLG00000032268 RLG00000032269 RLG00000032272 RLG00000032282 RLG00000032283 RLG00000032308 RLG00000032413 RLG00000033129 RLG00000034945
rosa_multiflora Rmu_co8241929.1_g000001 Rmu_co8269227.1_g000001 Rmu_co8461259.1_g000001 Rmu_co8495385.1_g000001 Rmu_sc0000028.1_g000003 Rmu_sc0000083.1_g000009 Rmu_sc0000083.1_g000010 Rmu_sc0000083.1_g000031 Rmu_sc0000083.1_g000043 Rmu_sc0000083.1_g000046 Rmu_sc0000083.1_g000047 Rmu_sc0000563.1_g000024 Rmu_sc0000842.1_g000012 Rmu_sc0001235.1_g000001 Rmu_sc0001235.1_g000005 Rmu_sc0001235.1_g000009 Rmu_sc0001235.1_g000015 Rmu_sc0001235.1_g000016 Rmu_sc0001235.1_g000017 Rmu_sc0001473.1_g000036 Rmu_sc0001607.1_g000016 Rmu_sc0001788.1_g000006 Rmu_sc0002018.1_g000008 Rmu_sc0002028.1_g000001 Rmu_sc0002680.1_g000011 Rmu_sc0002798.1_g000023 Rmu_sc0002886.1_g000023 Rmu_sc0002989.1_g000012 Rmu_sc0003064.1_g000009 Rmu_sc0003382.1_g000006 Rmu_sc0003630.1_g000002 Rmu_sc0003630.1_g000047 Rmu_sc0003630.1_g000066 Rmu_sc0003630.1_g000067 Rmu_sc0003649.1_g000010 Rmu_sc0003688.1_g000005 Rmu_sc0003920.1_g000007 Rmu_sc0004765.1_g000007 Rmu_sc0004765.1_g000008 Rmu_sc0004920.1_g000011 Rmu_sc0005223.1_g000008 Rmu_sc0005223.1_g000010 Rmu_sc0005223.1_g000015 Rmu_sc0005223.1_g000016 Rmu_sc0005319.1_g000014 Rmu_sc0005319.1_g000015 Rmu_sc0005319.1_g000018 Rmu_sc0005319.1_g000019 Rmu_sc0005319.1_g000025 Rmu_sc0005418.1_g000007 Rmu_sc0005418.1_g000012 Rmu_sc0006106.1_g000011 Rmu_sc0006914.1_g000012 Rmu_sc0007023.1_g000001 Rmu_sc0007662.1_g000001 Rmu_sc0007920.1_g000002 Rmu_sc0007920.1_g000013 Rmu_sc0008053.1_g000016 Rmu_sc0008053.1_g000017 Rmu_sc0008053.1_g000018 Rmu_sc0008053.1_g000023 Rmu_sc0008053.1_g000050 Rmu_sc0009042.1_g000007 Rmu_sc0009042.1_g000008 Rmu_sc0010642.1_g000008 Rmu_sc0011109.1_g000002 Rmu_sc0011314.1_g000006 Rmu_sc0011974.1_g000006 Rmu_sc0012179.1_g000009 Rmu_sc0015169.1_g000001 Rmu_sc0016368.1_g000002 Rmu_sc0022659.1_g000001 Rmu_sc0027371.1_g000001 Rmu_sc0028213.1_g000001 Rmu_sc0034526.1_g000001 Rmu_sc0039366.1_g000002 Rmu_ssc0000050.1_g000084 Rmu_ssc0000050.1_g000088 Rmu_ssc0000167.1_g000017
rosa_roxburghii Rroxscaffold_1G00011340 Rroxscaffold_1G00025710 Rroxscaffold_1G00025870 Rroxscaffold_1G00026070 Rroxscaffold_1G00026170 Rroxscaffold_1G00026200 Rroxscaffold_1G00026270 Rroxscaffold_1G00050950 Rroxscaffold_1G00058900 Rroxscaffold_1G00060040 Rroxscaffold_1G00060060 Rroxscaffold_1G00060070 Rroxscaffold_1G00060080 Rroxscaffold_1G00060110 Rroxscaffold_1G00060120 Rroxscaffold_1G00060140 Rroxscaffold_1G00060170 Rroxscaffold_1G00060210 Rroxscaffold_1G00060220 Rroxscaffold_1G00060240 Rroxscaffold_1G00060250 Rroxscaffold_1G00060260 Rroxscaffold_1G00060300 Rroxscaffold_1G00060320 Rroxscaffold_1G00060330 Rroxscaffold_1G00060900 Rroxscaffold_1G00060920 Rroxscaffold_1G00073980 Rroxscaffold_2G00077560 Rroxscaffold_2G00077580 Rroxscaffold_2G00146990 Rroxscaffold_3G00236200 Rroxscaffold_3G00236210 Rroxscaffold_3G00236220 Rroxscaffold_4G00292760 Rroxscaffold_4G00315200 Rroxscaffold_4G00315240 Rroxscaffold_4G00326240 Rroxscaffold_4G00328980 Rroxscaffold_5G00341890 Rroxscaffold_7G00161350 Rroxscaffold_7G00174490 Rroxscaffold_7G00206550
rosa_rugosa Rorug01G0093000 Rorug01G0093100 Rorug01G0128800 Rorug01G0312000 Rorug01G0312000 Rorug02G0581100 Rorug03G0224600 Rorug05G0028500 Rorug05G0032700 Rorug05G0032800 Rorug05G0032900 Rorug05G0032900 Rorug05G0033000 Rorug05G0033100 Rorug05G0033300 Rorug05G0033400 Rorug05G0033900 Rorug05G0034900 Rorug05G0035000 Rorug05G0046900 Rorug05G0047500 Rorug05G0047500 Rorug05G0111600 Rorug05G0287900 Rorug05G0288700 Rorug05G0586900 Rorug06G0231700 Rorug06G0354300 Rorug06G0354400
rosa_samantha Rh1DG122200 Rh2DG686800 Rh5BG136600 Rh6AG344500 Rh6BG351900 Rh6CG359000 Rh6CG359100 Rh6CG387600 Rh6CG480500 Rh6DG344600 Rh6DG467400
rosa_wichuraiana Rw0G010250 Rw0G014820 Rw0G021400 Rw0G023100 Rw1G009450 Rw1G012100 Rw1G012180 Rw1G028360 Rw2G054260 Rw3G024370 Rw3G024420 Rw3G024470 Rw5G010850 Rw5G011010 Rw5G011020 Rw5G011050 Rw5G011080 Rw5G011120 Rw5G012300 Rw5G018470 Rw5G033830 Rw6G008930 Rw6G030070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1190
AccI GTMKAC 1 cut(s) 1638
AccII CGCG 2 cut(s) 50, 704
AciI CCGC 6 cut(s) 20, 44, 48, 50, 702, 936
AcoI YGGCCR 3 cut(s) 45, 51, 1167
AcsI RAATTY 3 cut(s) 918, 953, 1752
AcuI CTGAAG 4 cut(s) 93, 285, 1524, 1953
AcvI CACGTG 1 cut(s) 2078
AdeI CACNNNGTG 1 cut(s) 2081
AfaI GTAC 6 cut(s) 24, 290, 664, 977, 1296, 1952
AfiI CCNNNNNNNGG 1 cut(s) 516
AflII CTTAAG 1 cut(s) 548
AgsI TTSAA 5 cut(s) 497, 607, 674, 1376, 1400
AhlI ACTAGT 1 cut(s) 1303
AjnI CCWGG 3 cut(s) 97, 258, 315
Alw21I GWGCWC 3 cut(s) 1344, 1414, 1572
Alw26I GTCTC 4 cut(s) 1034, 1280, 1784, 1817
Ama87I CYCGRG 2 cut(s) 658, 1571
AoxI GGCC 6 cut(s) 45, 51, 152, 731, 1167, 1850
ApeKI GCWGC 5 cut(s) 68, 102, 615, 894, 1469
ApoI RAATTY 3 cut(s) 918, 953, 1752
Asp700I GAANNNNTTC 1 cut(s) 696
AspLEI GCGC 1 cut(s) 286
AspS9I GGNCC 2 cut(s) 152, 731
AsuC2I CCSGG 2 cut(s) 511, 843
AsuHPI GGTGA 8 cut(s) 118, 281, 1237, 1273, 1312, 1956, 2012, 2093
AvaI CYCGRG 2 cut(s) 658, 1571
BaeGI GKGCMC 1 cut(s) 1195
BaeI ACNNNNGTAYC 2 cut(s) 1278, 1311
BalI TGGCCA 1 cut(s) 1169
BanI GGYRCC 1 cut(s) 1190
BauI CACGAG 1 cut(s) 1094
BbrPI CACGTG 1 cut(s) 2078
Bbv12I GWGCWC 3 cut(s) 1344, 1414, 1572
BbvI GCAGC 5 cut(s) 80, 89, 627, 906, 1481
BccI CCATC 3 cut(s) 349, 866, 2078
BceAI ACGGC 2 cut(s) 62, 335
BcgI CGANNNNNNTGC 2 cut(s) 395, 429
BciT130I CCWGG 3 cut(s) 99, 260, 317
BciVI GTATCC 1 cut(s) 1577
BclI TGATCA 1 cut(s) 1207
BcnI CCSGG 2 cut(s) 511, 843
BcoDI GTCTC 4 cut(s) 1034, 1280, 1784, 1817
BcuI ACTAGT 1 cut(s) 1303
BfaI CTAG 9 cut(s) 378, 540, 1271, 1304, 1415, 1484, 1589, 1658, 2013
BfmI CTRYAG 2 cut(s) 1007, 1639
BfrI CTTAAG 1 cut(s) 548
BfuI GTATCC 1 cut(s) 1577
BisI GCNGC 8 cut(s) 45, 48, 51, 69, 103, 616, 895, 1470
BlpI GCTNAGC 1 cut(s) 1705
BlsI GCNGC 8 cut(s) 46, 49, 52, 70, 104, 617, 896, 1471
BmcAI AGTACT 2 cut(s) 977, 1952
Bme1390I CCNGG 5 cut(s) 99, 260, 317, 511, 843
BmeT110I CYCGRG 2 cut(s) 658, 1571
BmgT120I GGNCC 2 cut(s) 152, 731
BmiI GGNNCC 3 cut(s) 857, 1192, 1536
BmrFI CCNGG 5 cut(s) 99, 260, 317, 511, 843
BmrI ACTGGG 1 cut(s) 2049
BmsI GCATC 8 cut(s) 264, 397, 452, 1691, 1722, 1753, 1915, 2061
BmuI ACTGGG 1 cut(s) 2049
Bpu1102I GCTNAGC 1 cut(s) 1705
BpuEI CTTGAG 2 cut(s) 72, 1091
BpuMI CCSGG 2 cut(s) 511, 843
BsaAI YACGTR 2 cut(s) 292, 2078
BsaJI CCNNGG 9 cut(s) 48, 316, 510, 880, 1108, 1324, 1728, 1872, 1910
BsaWI WCCGGW 1 cut(s) 458
BsaXI ACNNNNNCTCC 8 cut(s) 15, 45, 1020, 1050, 1412, 1442, 1934, 1964
Bsc4I CCNNNNNNNGG 1 cut(s) 516
Bse1I ACTGG 6 cut(s) 30, 573, 603, 895, 2032, 2044
Bse3DI GCAATG 2 cut(s) 225, 390
BseBI CCWGG 3 cut(s) 99, 260, 317
BseDI CCNNGG 9 cut(s) 48, 316, 510, 880, 1108, 1324, 1728, 1872, 1910
BseGI GGATG 7 cut(s) 197, 232, 467, 534, 1738, 1906, 1993
BseLI CCNNNNNNNGG 1 cut(s) 516
BseMI GCAATG 2 cut(s) 225, 390
BseMII CTCAG 2 cut(s) 577, 1047
BseNI ACTGG 6 cut(s) 30, 573, 603, 895, 2032, 2044
BseRI GAGGAG 4 cut(s) 23, 1420, 1955, 2010
BseSI GKGCMC 1 cut(s) 1195
BseX3I CGGCCG 2 cut(s) 45, 51
BseXI GCAGC 5 cut(s) 80, 89, 627, 906, 1481
BsgI GTGCAG 2 cut(s) 88, 456
Bsh1236I CGCG 2 cut(s) 50, 704
Bsh1285I CGRYCG 2 cut(s) 48, 54
BshFI GGCC 6 cut(s) 47, 53, 154, 733, 1169, 1852
BshNI GGYRCC 1 cut(s) 1190
BsiEI CGRYCG 2 cut(s) 48, 54
BsiHKAI GWGCWC 3 cut(s) 1344, 1414, 1572
BsiHKCI CYCGRG 2 cut(s) 658, 1571
BsiSI CCGG 3 cut(s) 459, 510, 842
BslFI GGGAC 1 cut(s) 1588
BslI CCNNNNNNNGG 1 cut(s) 516
BsmAI GTCTC 4 cut(s) 1034, 1280, 1784, 1817
BsmFI GGGAC 1 cut(s) 1588
BsmI GAATGC 1 cut(s) 1927
BsnI GGCC 6 cut(s) 47, 53, 154, 733, 1169, 1852
BsoBI CYCGRG 2 cut(s) 658, 1571
Bsp1286I GDGCHC 4 cut(s) 1195, 1344, 1414, 1572
Bsp143I GATC 4 cut(s) 815, 1207, 1358, 2002
Bsp1720I GCTNAGC 1 cut(s) 1705
Bsp19I CCATGG 4 cut(s) 1324, 1728, 1872, 1910
BspACI CCGC 6 cut(s) 20, 44, 48, 50, 702, 936
BspANI GGCC 6 cut(s) 47, 53, 154, 733, 1169, 1852
BspCNI CTCAG 2 cut(s) 576, 1046
BspFNI CGCG 2 cut(s) 50, 704
BspHI TCATGA 1 cut(s) 957
BspLI GGNNCC 3 cut(s) 857, 1192, 1536
BspT107I GGYRCC 1 cut(s) 1190
BspTI CTTAAG 1 cut(s) 548
BsrDI GCAATG 2 cut(s) 225, 390
BsrI ACTGG 6 cut(s) 30, 573, 603, 895, 2032, 2044
BssECI CCNNGG 9 cut(s) 48, 316, 510, 880, 1108, 1324, 1728, 1872, 1910
BssMI GATC 4 cut(s) 815, 1207, 1358, 2002
BssSI CACGAG 1 cut(s) 1094
BssT1I CCWWGG 6 cut(s) 880, 1108, 1324, 1728, 1872, 1910
Bst2BI CACGAG 1 cut(s) 1094
Bst2UI CCWGG 3 cut(s) 99, 260, 317
Bst4CI ACNGT 7 cut(s) 434, 501, 1132, 1368, 1530, 1795, 1976
Bst6I CTCTTC 1 cut(s) 438
BstAFI CTTAAG 1 cut(s) 548
BstBAI YACGTR 2 cut(s) 292, 2078
BstC8I GCNNGC 5 cut(s) 348, 769, 839, 1711, 2054
BstDEI CTNAG 4 cut(s) 563, 901, 1033, 1705
BstDSI CCRYGG 5 cut(s) 48, 1324, 1728, 1872, 1910
BstEII GGTNACC 2 cut(s) 428, 1962
BstF5I GGATG 7 cut(s) 197, 232, 467, 534, 1738, 1906, 1993
BstFNI CGCG 2 cut(s) 50, 704
BstHHI GCGC 1 cut(s) 286
BstKTI GATC 4 cut(s) 818, 1210, 1361, 2005
BstMAI GTCTC 4 cut(s) 1034, 1280, 1784, 1817
BstMBI GATC 4 cut(s) 815, 1207, 1358, 2002
BstMCI CGRYCG 2 cut(s) 48, 54
BstNI CCWGG 3 cut(s) 99, 260, 317
BstNSI RCATGY 3 cut(s) 469, 771, 2056
BstPI GGTNACC 2 cut(s) 428, 1962
BstSCI CCNGG 5 cut(s) 97, 258, 315, 509, 841
BstSFI CTRYAG 2 cut(s) 1007, 1639
BstSLI GKGCMC 1 cut(s) 1195
BstSNI TACGTA 1 cut(s) 292
BstUI CGCG 2 cut(s) 50, 704
BstV1I GCAGC 5 cut(s) 80, 89, 627, 906, 1481
BstZI CGGCCG 2 cut(s) 45, 51
BsuI GTATCC 1 cut(s) 1577
BsuRI GGCC 6 cut(s) 47, 53, 154, 733, 1169, 1852
BtgI CCRYGG 5 cut(s) 48, 1324, 1728, 1872, 1910
BtgZI GCGATG 4 cut(s) 223, 327, 420, 822
BtsCI GGATG 7 cut(s) 197, 232, 467, 534, 1738, 1906, 1993
BtsI GCAGTG 1 cut(s) 832
BtsIMutI CAGTG 5 cut(s) 105, 201, 687, 832, 1041
Cac8I GCNNGC 5 cut(s) 348, 769, 839, 1711, 2054
CciI TCATGA 1 cut(s) 957
CciNI GCGGCCGC 1 cut(s) 45
CfoI GCGC 1 cut(s) 286
Cfr13I GGNCC 2 cut(s) 152, 731
Cfr42I CCGCGG 1 cut(s) 51
Csp6I GTAC 6 cut(s) 23, 289, 663, 976, 1295, 1951
CspCI CAANNNNNGTGG 4 cut(s) 1662, 1697, 1996, 2031
CviQI GTAC 6 cut(s) 23, 289, 663, 976, 1295, 1951
DdeI CTNAG 4 cut(s) 563, 901, 1033, 1705
DpnI GATC 4 cut(s) 817, 1209, 1360, 2004
DpnII GATC 4 cut(s) 815, 1207, 1358, 2002
DraI TTTAAA 1 cut(s) 982
DraIII CACNNNGTG 1 cut(s) 2081
EaeI YGGCCR 3 cut(s) 45, 51, 1167
EagI CGGCCG 2 cut(s) 45, 51
Eam1104I CTCTTC 1 cut(s) 438
EarI CTCTTC 1 cut(s) 438
EclXI CGGCCG 2 cut(s) 45, 51
Eco105I TACGTA 1 cut(s) 292
Eco130I CCWWGG 6 cut(s) 880, 1108, 1324, 1728, 1872, 1910
Eco147I AGGCCT 1 cut(s) 1852
Eco52I CGGCCG 2 cut(s) 45, 51
Eco57I CTGAAG 4 cut(s) 93, 285, 1524, 1953
Eco72I CACGTG 1 cut(s) 2078
Eco88I CYCGRG 2 cut(s) 658, 1571
Eco91I GGTNACC 2 cut(s) 428, 1962
EcoO109I RGGNCCY 1 cut(s) 152
EcoO65I GGTNACC 2 cut(s) 428, 1962
EcoRII CCWGG 3 cut(s) 97, 258, 315
EcoT14I CCWWGG 6 cut(s) 880, 1108, 1324, 1728, 1872, 1910
EcoT22I ATGCAT 2 cut(s) 467, 769
ErhI CCWWGG 6 cut(s) 880, 1108, 1324, 1728, 1872, 1910
FalI AAGNNNNNCTT 2 cut(s) 635, 667
FaqI GGGAC 1 cut(s) 1588
FauI CCCGC 1 cut(s) 709
FauNDI CATATG 1 cut(s) 473
FbaI TGATCA 1 cut(s) 1207
FblI GTMKAC 1 cut(s) 1638
Fnu4HI GCNGC 8 cut(s) 45, 48, 51, 69, 103, 616, 895, 1470
FokI GGATG 7 cut(s) 184, 239, 474, 521, 1745, 1893, 2000
Fsp4HI GCNGC 8 cut(s) 45, 48, 51, 69, 103, 616, 895, 1470
FspBI CTAG 9 cut(s) 378, 540, 1271, 1304, 1415, 1484, 1589, 1658, 2013
GlaI GCGC 1 cut(s) 285
GluI GCNGC 8 cut(s) 45, 48, 51, 69, 103, 616, 895, 1470
HaeIII GGCC 6 cut(s) 47, 53, 154, 733, 1169, 1852
HapII CCGG 3 cut(s) 459, 510, 842
HhaI GCGC 1 cut(s) 286
Hin6I GCGC 1 cut(s) 284
HinP1I GCGC 1 cut(s) 284
HincII GTYRAC 1 cut(s) 1219
HindII GTYRAC 1 cut(s) 1219
HindIII AAGCTT 1 cut(s) 695
HinfI GANTC 3 cut(s) 706, 1104, 1810
HpaI GTTAAC 1 cut(s) 1219
HpaII CCGG 3 cut(s) 459, 510, 842
HphI GGTGA 8 cut(s) 118, 281, 1237, 1273, 1312, 1956, 2012, 2093
Hpy166II GTNNAC 4 cut(s) 648, 1135, 1219, 1639
Hpy188I TCNGA 5 cut(s) 425, 566, 798, 1513, 1972
Hpy188III TCNNGA 6 cut(s) 958, 1457, 1573, 1757, 1937, 2062
Hpy8I GTNNAC 4 cut(s) 648, 1135, 1219, 1639
HpyAV CCTTC 7 cut(s) 260, 632, 634, 1069, 1109, 1548, 1977
HpyCH4III ACNGT 7 cut(s) 434, 501, 1132, 1368, 1530, 1795, 1976
HpyCH4IV ACGT 3 cut(s) 291, 1803, 2077
HpyF3I CTNAG 4 cut(s) 563, 901, 1033, 1705
HpySE526I ACGT 3 cut(s) 291, 1803, 2077
HspAI GCGC 1 cut(s) 284
Ksp22I TGATCA 1 cut(s) 1207
KspAI GTTAAC 1 cut(s) 1219
KspI CCGCGG 1 cut(s) 51
Kzo9I GATC 4 cut(s) 815, 1207, 1358, 2002
LmnI GCTCC 4 cut(s) 512, 1409, 1520, 1663
Lsp1109I GCAGC 5 cut(s) 80, 89, 627, 906, 1481
LweI GCATC 8 cut(s) 264, 397, 452, 1691, 1722, 1753, 1915, 2061
MaeI CTAG 9 cut(s) 378, 540, 1271, 1304, 1415, 1484, 1589, 1658, 2013
MaeII ACGT 3 cut(s) 291, 1803, 2077
MalI GATC 4 cut(s) 817, 1209, 1360, 2004
MboI GATC 4 cut(s) 815, 1207, 1358, 2002
MboII GAAGA 6 cut(s) 68, 455, 647, 686, 1399, 1716
MhlI GDGCHC 4 cut(s) 1195, 1344, 1414, 1572
MlsI TGGCCA 1 cut(s) 1169
MluNI TGGCCA 1 cut(s) 1169
MlyI GAGTC 1 cut(s) 1819
MmeI TCCRAC 2 cut(s) 895, 1491
Mox20I TGGCCA 1 cut(s) 1169
Mph1103I ATGCAT 2 cut(s) 467, 769
MroXI GAANNNNTTC 1 cut(s) 696
MscI TGGCCA 1 cut(s) 1169
MseI TTAA 7 cut(s) 186, 549, 711, 981, 1218, 1745, 2021
MslI CAYNNNNRTG 3 cut(s) 928, 1254, 1458
Msp20I TGGCCA 1 cut(s) 1169
MspA1I CMGCKG 2 cut(s) 50, 71
MspCI CTTAAG 1 cut(s) 548
MspI CCGG 3 cut(s) 459, 510, 842
MspR9I CCNGG 5 cut(s) 99, 260, 317, 511, 843
Mva1269I GAATGC 1 cut(s) 1927
MvaI CCWGG 3 cut(s) 99, 260, 317
MvnI CGCG 2 cut(s) 50, 704
NciI CCSGG 2 cut(s) 511, 843
NcoI CCATGG 4 cut(s) 1324, 1728, 1872, 1910
NdeI CATATG 1 cut(s) 473
NdeII GATC 4 cut(s) 815, 1207, 1358, 2002
NlaIV GGNNCC 3 cut(s) 857, 1192, 1536
NmuCI GTSAC 4 cut(s) 476, 830, 1318, 1962
NotI GCGGCCGC 1 cut(s) 45
NsiI ATGCAT 2 cut(s) 467, 769
NspI RCATGY 3 cut(s) 469, 771, 2056
PaeI GCATGC 2 cut(s) 771, 2056
PagI TCATGA 1 cut(s) 957
PceI AGGCCT 1 cut(s) 1852
PcsI WCGNNNNNNNCGW 1 cut(s) 297
PctI GAATGC 1 cut(s) 1927
PdmI GAANNNNTTC 1 cut(s) 696
PfeI GAWTC 2 cut(s) 706, 1104
PkrI GCNGC 8 cut(s) 46, 49, 52, 70, 104, 617, 896, 1471
PleI GAGTC 1 cut(s) 1818
PmaCI CACGTG 1 cut(s) 2078
PmlI CACGTG 1 cut(s) 2078
PpsI GAGTC 1 cut(s) 1818
Ppu21I YACGTR 2 cut(s) 292, 2078
Psp6I CCWGG 3 cut(s) 97, 258, 315
PspCI CACGTG 1 cut(s) 2078
PspEI GGTNACC 2 cut(s) 428, 1962
PspGI CCWGG 3 cut(s) 97, 258, 315
PspN4I GGNNCC 3 cut(s) 857, 1192, 1536
PspPI GGNCC 2 cut(s) 152, 731
PvuII CAGCTG 1 cut(s) 71
RsaI GTAC 6 cut(s) 24, 290, 664, 977, 1296, 1952
RsaNI GTAC 6 cut(s) 23, 289, 663, 976, 1295, 1951
RseI CAYNNNNRTG 3 cut(s) 928, 1254, 1458
SacII CCGCGG 1 cut(s) 51
SaqAI TTAA 7 cut(s) 186, 549, 711, 981, 1218, 1745, 2021
SatI GCNGC 8 cut(s) 45, 48, 51, 69, 103, 616, 895, 1470
Sau3AI GATC 4 cut(s) 815, 1207, 1358, 2002
Sau96I GGNCC 2 cut(s) 152, 731
ScaI AGTACT 2 cut(s) 977, 1952
SchI GAGTC 1 cut(s) 1819
ScrFI CCNGG 5 cut(s) 99, 260, 317, 511, 843
SduI GDGCHC 4 cut(s) 1195, 1344, 1414, 1572
SfaNI GCATC 8 cut(s) 264, 397, 452, 1691, 1722, 1753, 1915, 2061
SfcI CTRYAG 2 cut(s) 1007, 1639
Sfr303I CCGCGG 1 cut(s) 51
SgrBI CCGCGG 1 cut(s) 51
SmiMI CAYNNNNRTG 3 cut(s) 928, 1254, 1458
SmlI CTYRAG 3 cut(s) 87, 548, 1070
SmoI CTYRAG 3 cut(s) 87, 548, 1070
SnaBI TACGTA 1 cut(s) 292
SpeI ACTAGT 1 cut(s) 1303
SphI GCATGC 2 cut(s) 771, 2056
SseBI AGGCCT 1 cut(s) 1852
SsiI CCGC 6 cut(s) 20, 44, 48, 50, 702, 936
SspI AATATT 1 cut(s) 1601
SspMI CTAG 9 cut(s) 378, 540, 1271, 1304, 1415, 1484, 1589, 1658, 2013
StuI AGGCCT 1 cut(s) 1852
StyD4I CCNGG 5 cut(s) 97, 258, 315, 509, 841
StyI CCWWGG 6 cut(s) 880, 1108, 1324, 1728, 1872, 1910
TaaI ACNGT 7 cut(s) 434, 501, 1132, 1368, 1530, 1795, 1976
TaiI ACGT 3 cut(s) 294, 1806, 2080
TaqI TCGA 1 cut(s) 1456
TatI WGTACW 3 cut(s) 662, 975, 1950
TauI GCSGC 3 cut(s) 47, 50, 53
TfiI GAWTC 2 cut(s) 706, 1104
Tru1I TTAA 7 cut(s) 186, 549, 711, 981, 1218, 1745, 2021
Tru9I TTAA 7 cut(s) 186, 549, 711, 981, 1218, 1745, 2021
TscAI CASTG 5 cut(s) 112, 208, 694, 839, 1041
TseFI GTSAC 4 cut(s) 476, 830, 1318, 1962
TseI GCWGC 5 cut(s) 68, 102, 615, 894, 1469
Tsp45I GTSAC 4 cut(s) 476, 830, 1318, 1962
TspRI CASTG 5 cut(s) 112, 208, 694, 839, 1041
Vha464I CTTAAG 1 cut(s) 548
XapI RAATTY 3 cut(s) 918, 953, 1752
XceI RCATGY 3 cut(s) 469, 771, 2056
XcmI CCANNNNNNNNNTGG 2 cut(s) 219, 887
XmiI GTMKAC 1 cut(s) 1638
XmnI GAANNNNTTC 1 cut(s) 696
XspI CTAG 9 cut(s) 378, 540, 1271, 1304, 1415, 1484, 1589, 1658, 2013
ZrmI AGTACT 2 cut(s) 977, 1952
Zsp2I ATGCAT 2 cut(s) 467, 769
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.