Rroxscaffold_1G00060330

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000066
Physical Location & Seq
Forward (+)
82583922 .. 82586428
2507 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00060330.1

Sequence Viewer

Length: 2283 bp
ATGGCCTTTCTTCAAAAGATGCTTATTTTGCAGCTTTCAGTCTTTTCTGTAATATTAGCAACTGCAACACCAGCAGTAGGTCAAGCTTTGCCCGGCTGCCCCGACCGCTGTGGCGATTTGTTAATTCCATACCCTTTTGGCATAGCTGAAGGTTGTCACTTGGGAGATGAGTTCTTCATCAACTGCACTACTGAAGTGACAGGAACATCCCCAACACCATATCTGGCAGGAACTGAAATCCCAGTTTCCAACATTTTTCTTGATCAAGGTGAGTTACAAATAATGCAGTTTGTAGCCAGAGACTGTTATGACAGCCAGGGCTATTTAGATAAGAAACTCAGCAAAGTACGAATCTTGAGCCTCAATCCTCCTTTCACAATCTCTGGCGCCAAAAACAAGTTCTTTGCTGTTGGTTGTGACACTTATGCTATCTTCGAAGGCTATCGAGGCCATGAAAGGTACATAACTGGCTGCATGTCCTTCTGTGAGAGCCTTGGGAGTGTTAATGAGTCTTGTTCTGGTATAGGGTGTTGCCAGACTTTAATCCCAACTGGACTGCAAAACCGCACAGTGAAGATGAATAGCTACTATAATCATGCTTTCATATGGGACTTTAACCCCTGCAGCTACTCCTTCATTGTGGAAGACGGCCAGTTTGAATTCTCTAGTAAAAGTTTTCAAGTACTAAACCAGACAAGCCGGCTTCCGATGGTTCTGAATTGGCAAATTGGGAATGAGACATGTGATGAAGCTCAAAAGAAGCAAGGTTATGCTTGTAAGGGAAATAGCACATGTGTCAACCCCATCAATCTGTCTGGTTACTTCTGTCAATGCTTGCCAGGTTATGAAGGGAATCCATACCTACCAGATGGTTGCCATGATATAAATGAGTGCAAGAATTCAAACATCTGCAGTGAAGGAGCGTGTGTAAACTTCCCAGGAACTTATGCTTGTGTATGTCCCAAAGGGTTCGAAGGCGATGGAAGGAAAGCCGGAACGGGTTGCAGGAAAGACAATCCCATGAATCCTCATCAGACTTCTCGCTTGCTCATTATTTCGCTAGGTATGAGTGTAGCCTTCTTGTTCATAATGGTTGGAAGTTCATGGACATACTTGGGAGTGAAGAAAAGGAAATTCATCCAACTGAGAGAAAAGTATTTCAAAGAAAATGGTGGCTTATTGCTACAACAGAAACTCGCTAATCACGAAGGAGCTGTGCAGACAACAAAAATATTTACTGCAGAAGAACTTGAGAAGGCTACAAACAACTACCATGAAGATAGAGTTGTTGGTGAAGGAGGCTTTGGAACTGTTTATAAAGGGACATTAGCAGATGGCAAGGTGGTTGCTATAAAGAAGTCAAAAATCAGTGCCCCAAGCCAGAGTGAACAGTTTGTTAATGAGGTGATTGTTCTTTCTCAAGTCAACCATAGAAATGTGGTGAGGCTATTAGGATGTTGTTTCGAAACACCAGTGCCTCTATTGGTTTACGAGTTCATCACCAATGGCACTCTTTTTGAGCACATACATAATAACAAGGGCGAAAAATCACCACTTACGTGGGAATTACGTTTGAAGATAGCAGCAGAAATTGCCGGAGCATTAGCATACTTACACACCTCCATTTCCATGCCAATCATTCACCGAGACGTGAAAGCAACAAATGTACTGATAGATGACAATTACACCGCAAAAGTGTCGGATTTTGGAGCTTCAAGGTTGGTTCCTCTAGATCAAACTCAAATAACAACTTTGGTACAAGGGACACTCGGATACTTGGACCCCGAATACTTTCATTCAAACCAACTAACAGAAAAGAGTGACGTCTATAGCTTCGGAGTTGTCCTAGTGGAGCTACTTACAAGCAAATTAGCACTTTCTTTTACAAGGCCTGAGGCAGAGAGGAACCTAGCAAGCTTCTTTGTTTGTTCACTGGAAGACGGTCACTTGAATCAAATTCTCGACCAAAACATACTCAATGAGGTGAATATTGAGATGCTAAGAGAAGTAGCCAACGTGGCAAGAAGATGTTTAAGGGTAACAAGGGAGGAAAGGCCTACCATGAAAGAAGTATCAATGGAGCTGGAGGGAATGAGGATCAGTGCAAAGCATCCATGGGGAAAGACTGGTTTCAGCCCCGAGGACACTGAACACTTGCTTGGATCACCTTCATTTTCCTATGTTACAAGTGATTGTGGTCCGAATAGTGCAACTATCAGTGCAGCTAGTGTTTATGAAAGCATGCGAATGGAAATGTTAATGGCATATGATAACGGGCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK hEGF

Protein Analysis

760

Amino Acids

84.17

Weight (kDa)

5.75

Isoelectric Point (pI)

33.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 33 - 94 3.5e-07 Wall-associated receptor kinase galacturonan-binding
EGF_CA PF07645 294 - 328 7.6e-07 Calcium-binding EGF domain
Pkinase PF00069 424 - 691 9.9e-48 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 427 - 695 4.9e-48 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21210 AT1G21230 AT1G21240 AT1G21240 AT1G21245 AT1G21250 AT1G21270 AT1G22720 AT3G25490
fragaria_vesca FvH4_2g35051 FvH4_2g35051 FvH4_2g35060 FvH4_3g10281 FvH4_3g10285 FvH4_3g10286 FvH4_3g10311 FvH4_3g10311 FvH4_3g10320 FvH4_3g10332 FvH4_3g10340 FvH4_3g10350 FvH4_3g10360 FvH4_3g10390 FvH4_3g10412 FvH4_3g10413 FvH4_3g10461 FvH4_3g10462 FvH4_3g10470 FvH4_3g10510 FvH4_3g10520 FvH4_3g10530 FvH4_3g10550 FvH4_3g10560 FvH4_3g10611 FvH4_3g11730 FvH4_6g53140 FvH4_6g53142 FvH4_7g31880 FvH4_7g31910 FvH4_7g31920
malus_domestica MD05G1269900.v1.1 MD05G1270000.v1.1 MD05G1270200.v1.1 MD05G1270400.v1.1 MD05G1270500.v1.1 MD05G1270600.v1.1 MD05G1270800.v1.1 MD05G1270900.v1.1 MD07G1183800.v1.1 MD08G1107600.v1.1 MD10G1248500.v1.1 MD10G1249200.v1.1 MD10G1249500.v1.1 MD10G1250000.v1.1 MD10G1250500.v1.1 MD10G1250900.v1.1 MD10G1251200.v1.1 MD10G1251400.v1.1 MD13G1004600.v1.1 MD15G1088400.v1.1
prunus_persica Prupe.1G442400_v2.0.a1 Prupe.1G442500_v2.0.a1 Prupe.2G223400_v2.0.a1 Prupe.4G091900_v2.0.a1 Prupe.4G093200_v2.0.a1 Prupe.4G093300_v2.0.a1 Prupe.4G093400_v2.0.a1 Prupe.4G093500_v2.0.a1 Prupe.4G094100_v2.0.a1 Prupe.4G094200_v2.0.a1 Prupe.4G094300_v2.0.a1 Prupe.6G358300_v2.0.a1
pyrus_communis pycom08g08990 pycom10g20660 pycom10g20670 pycom10g20680 pycom10g20710 pycom10g20740 pycom13g00400 pycom15g08290
rosa_chinensis RchiOBHm_Chr1g0326861 RchiOBHm_Chr1g0326871 RchiOBHm_Chr1g0331391 RchiOBHm_Chr1g0336911 RchiOBHm_Chr1g0364001 RchiOBHm_Chr2g0174541 RchiOBHm_Chr2g0175041 RchiOBHm_Chr2g0175101 RchiOBHm_Chr2g0175121 RchiOBHm_Chr3g0486331 RchiOBHm_Chr5g0015581 RchiOBHm_Chr5g0016551 RchiOBHm_Chr5g0016561 RchiOBHm_Chr5g0016581 RchiOBHm_Chr5g0016601 RchiOBHm_Chr5g0016611 RchiOBHm_Chr5g0016641 RchiOBHm_Chr5g0016661 RchiOBHm_Chr5g0016691 RchiOBHm_Chr5g0016701 RchiOBHm_Chr5g0016731 RchiOBHm_Chr5g0016741 RchiOBHm_Chr5g0016811 RchiOBHm_Chr5g0016841 RchiOBHm_Chr5g0016871 RchiOBHm_Chr5g0016881 RchiOBHm_Chr5g0016931 RchiOBHm_Chr5g0017021 RchiOBHm_Chr5g0017041 RchiOBHm_Chr5g0017071 RchiOBHm_Chr5g0017091 RchiOBHm_Chr5g0017121 RchiOBHm_Chr5g0017141 RchiOBHm_Chr5g0017291 RchiOBHm_Chr5g0017321 RchiOBHm_Chr5g0017401 RchiOBHm_Chr5g0019001 RchiOBHm_Chr5g0019071 RchiOBHm_Chr5g0028941 RchiOBHm_Chr5g0052141 RchiOBHm_Chr5g0054721 RchiOBHm_Chr5g0054801 RchiOBHm_Chr5g0054931 RchiOBHm_Chr5g0054941 RchiOBHm_Chr6g0259291 RchiOBHm_Chr6g0292991 RchiOBHm_Chr6g0306911
rosa_laevigata RLG00000009465 RLG00000009468 RLG00000010755 RLG00000011976 RLG00000014541 RLG00000014542 RLG00000015169 RLG00000022308 RLG00000027529 RLG00000029324 RLG00000030244 RLG00000030245 RLG00000032210 RLG00000032211 RLG00000032257 RLG00000032259 RLG00000032260 RLG00000032261 RLG00000032262 RLG00000032268 RLG00000032269 RLG00000032272 RLG00000032282 RLG00000032283 RLG00000032308 RLG00000032413 RLG00000033129 RLG00000034945
rosa_multiflora Rmu_co8241929.1_g000001 Rmu_co8269227.1_g000001 Rmu_co8461259.1_g000001 Rmu_co8495385.1_g000001 Rmu_sc0000028.1_g000003 Rmu_sc0000083.1_g000009 Rmu_sc0000083.1_g000010 Rmu_sc0000083.1_g000031 Rmu_sc0000083.1_g000043 Rmu_sc0000083.1_g000046 Rmu_sc0000083.1_g000047 Rmu_sc0000563.1_g000024 Rmu_sc0000842.1_g000012 Rmu_sc0001235.1_g000001 Rmu_sc0001235.1_g000005 Rmu_sc0001235.1_g000009 Rmu_sc0001235.1_g000015 Rmu_sc0001235.1_g000016 Rmu_sc0001235.1_g000017 Rmu_sc0001473.1_g000036 Rmu_sc0001607.1_g000016 Rmu_sc0001788.1_g000006 Rmu_sc0002018.1_g000008 Rmu_sc0002028.1_g000001 Rmu_sc0002680.1_g000011 Rmu_sc0002798.1_g000023 Rmu_sc0002886.1_g000023 Rmu_sc0002989.1_g000012 Rmu_sc0003064.1_g000009 Rmu_sc0003382.1_g000006 Rmu_sc0003630.1_g000002 Rmu_sc0003630.1_g000047 Rmu_sc0003630.1_g000066 Rmu_sc0003630.1_g000067 Rmu_sc0003649.1_g000010 Rmu_sc0003688.1_g000005 Rmu_sc0003920.1_g000007 Rmu_sc0004765.1_g000007 Rmu_sc0004765.1_g000008 Rmu_sc0004920.1_g000011 Rmu_sc0005223.1_g000008 Rmu_sc0005223.1_g000010 Rmu_sc0005223.1_g000015 Rmu_sc0005223.1_g000016 Rmu_sc0005319.1_g000014 Rmu_sc0005319.1_g000015 Rmu_sc0005319.1_g000018 Rmu_sc0005319.1_g000019 Rmu_sc0005319.1_g000025 Rmu_sc0005418.1_g000007 Rmu_sc0005418.1_g000012 Rmu_sc0006106.1_g000011 Rmu_sc0006914.1_g000012 Rmu_sc0007023.1_g000001 Rmu_sc0007662.1_g000001 Rmu_sc0007920.1_g000002 Rmu_sc0007920.1_g000013 Rmu_sc0008053.1_g000016 Rmu_sc0008053.1_g000017 Rmu_sc0008053.1_g000018 Rmu_sc0008053.1_g000023 Rmu_sc0008053.1_g000050 Rmu_sc0009042.1_g000007 Rmu_sc0009042.1_g000008 Rmu_sc0010642.1_g000008 Rmu_sc0011109.1_g000002 Rmu_sc0011314.1_g000006 Rmu_sc0011974.1_g000006 Rmu_sc0012179.1_g000009 Rmu_sc0015169.1_g000001 Rmu_sc0016368.1_g000002 Rmu_sc0022659.1_g000001 Rmu_sc0027371.1_g000001 Rmu_sc0028213.1_g000001 Rmu_sc0034526.1_g000001 Rmu_sc0039366.1_g000002 Rmu_ssc0000050.1_g000084 Rmu_ssc0000050.1_g000088 Rmu_ssc0000167.1_g000017
rosa_roxburghii Rroxscaffold_1G00011340 Rroxscaffold_1G00025710 Rroxscaffold_1G00025870 Rroxscaffold_1G00026070 Rroxscaffold_1G00026170 Rroxscaffold_1G00026200 Rroxscaffold_1G00026270 Rroxscaffold_1G00050950 Rroxscaffold_1G00058900 Rroxscaffold_1G00060040 Rroxscaffold_1G00060060 Rroxscaffold_1G00060070 Rroxscaffold_1G00060080 Rroxscaffold_1G00060110 Rroxscaffold_1G00060120 Rroxscaffold_1G00060140 Rroxscaffold_1G00060170 Rroxscaffold_1G00060210 Rroxscaffold_1G00060220 Rroxscaffold_1G00060240 Rroxscaffold_1G00060250 Rroxscaffold_1G00060260 Rroxscaffold_1G00060300 Rroxscaffold_1G00060320 Rroxscaffold_1G00060330 Rroxscaffold_1G00060900 Rroxscaffold_1G00060920 Rroxscaffold_1G00073980 Rroxscaffold_2G00077560 Rroxscaffold_2G00077580 Rroxscaffold_2G00146990 Rroxscaffold_3G00236200 Rroxscaffold_3G00236210 Rroxscaffold_3G00236220 Rroxscaffold_4G00292760 Rroxscaffold_4G00315200 Rroxscaffold_4G00315240 Rroxscaffold_4G00326240 Rroxscaffold_4G00328980 Rroxscaffold_5G00341890 Rroxscaffold_7G00161350 Rroxscaffold_7G00174490 Rroxscaffold_7G00206550
rosa_rugosa Rorug01G0093000 Rorug01G0093100 Rorug01G0128800 Rorug01G0312000 Rorug01G0312000 Rorug02G0581100 Rorug03G0224600 Rorug05G0028500 Rorug05G0032700 Rorug05G0032800 Rorug05G0032900 Rorug05G0032900 Rorug05G0033000 Rorug05G0033100 Rorug05G0033300 Rorug05G0033400 Rorug05G0033900 Rorug05G0034900 Rorug05G0035000 Rorug05G0046900 Rorug05G0047500 Rorug05G0047500 Rorug05G0111600 Rorug05G0287900 Rorug05G0288700 Rorug05G0586900 Rorug06G0231700 Rorug06G0354300 Rorug06G0354400
rosa_samantha Rh1DG122200 Rh2DG686800 Rh5BG136600 Rh6AG344500 Rh6BG351900 Rh6CG359000 Rh6CG359100 Rh6CG387600 Rh6CG480500 Rh6DG344600 Rh6DG467400
rosa_wichuraiana Rw0G010250 Rw0G014820 Rw0G021400 Rw0G023100 Rw1G009450 Rw1G012100 Rw1G012180 Rw1G028360 Rw2G054260 Rw3G024370 Rw3G024420 Rw3G024470 Rw5G010850 Rw5G011010 Rw5G011020 Rw5G011050 Rw5G011080 Rw5G011120 Rw5G012300 Rw5G018470 Rw5G033830 Rw6G008930 Rw6G030070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1317
AatII GACGTC 1 cut(s) 1827
AccB1I GGYRCC 1 cut(s) 386
AciI CCGC 3 cut(s) 106, 565, 1689
AclWI GGATC 2 cut(s) 2105, 2170
AcoI YGGCCR 1 cut(s) 649
AcsI RAATTY 4 cut(s) 659, 898, 1133, 1956
AcuI CTGAAG 2 cut(s) 168, 213
AcyI GRCGYC 2 cut(s) 387, 1824
AfaI GTAC 5 cut(s) 348, 461, 684, 1668, 1758
AfiI CCNNNNNNNGG 1 cut(s) 77
AflIII ACRYGT 2 cut(s) 740, 791
AgsI TTSAA 9 cut(s) 14, 659, 680, 903, 1162, 1576, 1716, 1800, 1951
AjiI CACGTC 1 cut(s) 1651
AjnI CCWGG 3 cut(s) 315, 838, 937
AjuI GAANNNNNNNTTGG 6 cut(s) 383, 415, 1287, 1319, 2142, 2174
AleI CACNNNNGTG 1 cut(s) 1558
Alw21I GWGCWC 1 cut(s) 1524
Alw26I GTCTC 3 cut(s) 294, 731, 1641
AlwI GGATC 2 cut(s) 2105, 2170
AlwNI CAGNNNCTG 2 cut(s) 233, 303
Ama87I CYCGRG 1 cut(s) 2138
AoxI GGCC 5 cut(s) 3, 448, 649, 1889, 2054
ApeKI GCWGC 6 cut(s) 31, 96, 471, 624, 1583, 2222
ApoI RAATTY 4 cut(s) 659, 898, 1133, 1956
ArsI GACNNNNNNTTYG 2 cut(s) 638, 670
Asp700I GAANNNNTTC 1 cut(s) 1791
AspLEI GCGC 1 cut(s) 389
AspS9I GGNCC 2 cut(s) 1780, 2198
AsuC2I CCSGG 1 cut(s) 93
AsuHPI GGTGA 9 cut(s) 281, 1304, 1417, 1453, 1492, 1542, 1634, 1996, 2157
AsuII TTCGAA 3 cut(s) 435, 972, 1464
AvaI CYCGRG 1 cut(s) 2138
AvaII GGWCC 2 cut(s) 1780, 2198
AxyI CCTNAGG 1 cut(s) 1893
BaeGI GKGCMC 1 cut(s) 1375
BanI GGYRCC 1 cut(s) 386
BbsI GAAGAC 2 cut(s) 651, 1944
Bbv12I GWGCWC 1 cut(s) 1524
BbvI GCAGC 6 cut(s) 43, 83, 458, 636, 1595, 2234
BccI CCATC 5 cut(s) 703, 812, 863, 974, 1328
BceAI ACGGC 1 cut(s) 664
BcgI CGANNNNNNTGC 2 cut(s) 1680, 1714
BciT130I CCWGG 3 cut(s) 317, 840, 939
BciVI GTATCC 1 cut(s) 1766
BclI TGATCA 1 cut(s) 262
BcnI CCSGG 1 cut(s) 93
BcoDI GTCTC 3 cut(s) 294, 731, 1641
BfaI CTAG 6 cut(s) 666, 1061, 1730, 1847, 1910, 2226
BfmI CTRYAG 4 cut(s) 622, 910, 1239, 1828
BfoI RGCGCY 1 cut(s) 390
BfuI GTATCC 1 cut(s) 1766
BglI GCCNNNNNGGC 1 cut(s) 2018
BisI GCNGC 6 cut(s) 32, 97, 472, 625, 1584, 2223
BlsI GCNGC 6 cut(s) 33, 98, 473, 626, 1585, 2224
BmcAI AGTACT 1 cut(s) 684
Bme1390I CCNGG 4 cut(s) 93, 317, 840, 939
Bme18I GGWCC 2 cut(s) 1780, 2198
BmeT110I CYCGRG 1 cut(s) 2138
BmgBI CACGTC 1 cut(s) 1651
BmgT120I GGNCC 2 cut(s) 1780, 2198
BmiI GGNNCC 4 cut(s) 388, 1725, 1782, 1907
BmrFI CCNGG 4 cut(s) 93, 317, 840, 939
BmrI ACTGGG 1 cut(s) 236
BmsI GCATC 3 cut(s) 9, 1986, 2119
BmuI ACTGGG 1 cut(s) 236
BpiI GAAGAC 2 cut(s) 651, 1944
BpmI CTGGAG 1 cut(s) 2105
Bpu14I TTCGAA 3 cut(s) 435, 972, 1464
BpuEI CTTGAG 3 cut(s) 376, 1271, 1404
BpuMI CCSGG 1 cut(s) 93
BsaAI YACGTR 1 cut(s) 1560
BsaHI GRCGYC 2 cut(s) 387, 1824
BsaJI CCNNGG 5 cut(s) 316, 493, 937, 2114, 2139
BsaXI ACNNNNNCTCC 2 cut(s) 912, 942
Bsc4I CCNNNNNNNGG 1 cut(s) 77
Bse118I RCCGGY 1 cut(s) 699
Bse1I ACTGG 7 cut(s) 242, 472, 556, 652, 1472, 1938, 2131
Bse21I CCTNAGG 1 cut(s) 1893
BseBI CCWGG 3 cut(s) 317, 840, 939
BseDI CCNNGG 5 cut(s) 316, 493, 937, 2114, 2139
BseGI GGATG 4 cut(s) 206, 1137, 1460, 2110
BseLI CCNNNNNNNGG 1 cut(s) 77
BseMII CTCAG 3 cut(s) 352, 1136, 1884
BseNI ACTGG 7 cut(s) 242, 472, 556, 652, 1472, 1938, 2131
BseSI GKGCMC 1 cut(s) 1375
BseXI GCAGC 6 cut(s) 43, 83, 458, 636, 1595, 2234
BsgI GTGCAG 3 cut(s) 169, 1238, 2241
Bsh1285I CGRYCG 1 cut(s) 106
BshFI GGCC 5 cut(s) 5, 450, 651, 1891, 2056
BshNI GGYRCC 1 cut(s) 386
BsiEI CGRYCG 1 cut(s) 106
BsiHKAI GWGCWC 1 cut(s) 1524
BsiHKCI CYCGRG 1 cut(s) 2138
BsiSI CCGG 4 cut(s) 93, 700, 993, 1596
BslFI GGGAC 4 cut(s) 623, 945, 1336, 1777
BslI CCNNNNNNNGG 1 cut(s) 77
BsmAI GTCTC 3 cut(s) 294, 731, 1641
BsmBI CGTCTC 1 cut(s) 1641
BsmFI GGGAC 4 cut(s) 623, 945, 1336, 1777
BsnI GGCC 5 cut(s) 5, 450, 651, 1891, 2056
BsoBI CYCGRG 1 cut(s) 2138
Bsp119I TTCGAA 3 cut(s) 435, 972, 1464
Bsp1286I GDGCHC 2 cut(s) 1375, 1524
Bsp143I GATC 4 cut(s) 262, 1732, 2097, 2162
Bsp19I CCATGG 1 cut(s) 2114
BspACI CCGC 3 cut(s) 106, 565, 1689
BspANI GGCC 5 cut(s) 5, 450, 651, 1891, 2056
BspCNI CTCAG 3 cut(s) 351, 1137, 1885
BspLI GGNNCC 4 cut(s) 388, 1725, 1782, 1907
BspMAI CTGCAG 3 cut(s) 626, 914, 1243
BspPI GGATC 2 cut(s) 2105, 2170
BspT104I TTCGAA 3 cut(s) 435, 972, 1464
BspT107I GGYRCC 1 cut(s) 386
BsrFI RCCGGY 1 cut(s) 699
BsrI ACTGG 7 cut(s) 242, 472, 556, 652, 1472, 1938, 2131
BssAI RCCGGY 1 cut(s) 699
BssECI CCNNGG 5 cut(s) 316, 493, 937, 2114, 2139
BssMI GATC 4 cut(s) 262, 1732, 2097, 2162
BssNI GRCGYC 2 cut(s) 387, 1824
BssT1I CCWWGG 2 cut(s) 493, 2114
Bst2UI CCWGG 3 cut(s) 317, 840, 939
Bst4CI ACNGT 5 cut(s) 305, 571, 1312, 1392, 1943
BstACI GRCGYC 2 cut(s) 387, 1824
BstAPI GCANNNNNTGC 1 cut(s) 1592
BstBAI YACGTR 1 cut(s) 1560
BstBI TTCGAA 3 cut(s) 435, 972, 1464
BstC8I GCNNGC 5 cut(s) 701, 836, 1046, 1915, 2243
BstDEI CTNAG 4 cut(s) 338, 1145, 1893, 2000
BstDSI CCRYGG 1 cut(s) 2114
BstF5I GGATG 4 cut(s) 206, 1137, 1460, 2110
BstH2I RGCGCY 1 cut(s) 390
BstHHI GCGC 1 cut(s) 389
BstKTI GATC 4 cut(s) 265, 1735, 2100, 2165
BstMAI GTCTC 3 cut(s) 294, 731, 1641
BstMBI GATC 4 cut(s) 262, 1732, 2097, 2162
BstMCI CGRYCG 1 cut(s) 106
BstMWI GCNNNNNNNGC 6 cut(s) 28, 71, 105, 447, 1592, 2018
BstNI CCWGG 3 cut(s) 317, 840, 939
BstNSI RCATGY 4 cut(s) 478, 744, 795, 2245
BstSCI CCNGG 4 cut(s) 91, 315, 838, 937
BstSFI CTRYAG 4 cut(s) 622, 910, 1239, 1828
BstSLI GKGCMC 1 cut(s) 1375
BstV1I GCAGC 6 cut(s) 43, 83, 458, 636, 1595, 2234
BstV2I GAAGAC 2 cut(s) 651, 1944
BstXI CCANNNNNNTGG 1 cut(s) 1560
Bsu36I CCTNAGG 1 cut(s) 1893
BsuI GTATCC 1 cut(s) 1766
BsuRI GGCC 5 cut(s) 5, 450, 651, 1891, 2056
BtgI CCRYGG 1 cut(s) 2114
BtgZI GCGATG 1 cut(s) 993
BtrI CACGTC 1 cut(s) 1651
BtsCI GGATG 4 cut(s) 206, 1137, 1460, 2110
BtsI GCAGTG 1 cut(s) 919
BtsIMutI CAGTG 8 cut(s) 576, 919, 1375, 1479, 1931, 2107, 2145, 2224
Cac8I GCNNGC 5 cut(s) 701, 836, 1046, 1915, 2243
CaiI CAGNNNCTG 2 cut(s) 233, 303
CfoI GCGC 1 cut(s) 389
Cfr10I RCCGGY 1 cut(s) 699
Cfr13I GGNCC 2 cut(s) 1780, 2198
Csp6I GTAC 5 cut(s) 347, 460, 683, 1667, 1757
CviQI GTAC 5 cut(s) 347, 460, 683, 1667, 1757
DdeI CTNAG 4 cut(s) 338, 1145, 1893, 2000
DinI GGCGCC 1 cut(s) 388
DpnI GATC 4 cut(s) 264, 1734, 2099, 2164
DpnII GATC 4 cut(s) 262, 1732, 2097, 2162
EaeI YGGCCR 1 cut(s) 649
Eco130I CCWWGG 2 cut(s) 493, 2114
Eco147I AGGCCT 2 cut(s) 1891, 2056
Eco47I GGWCC 2 cut(s) 1780, 2198
Eco57I CTGAAG 2 cut(s) 168, 213
Eco81I CCTNAGG 1 cut(s) 1893
Eco88I CYCGRG 1 cut(s) 2138
EcoRI GAATTC 2 cut(s) 659, 898
EcoRII CCWGG 3 cut(s) 315, 838, 937
EcoT14I CCWWGG 2 cut(s) 493, 2114
EgeI GGCGCC 1 cut(s) 388
EheI GGCGCC 1 cut(s) 388
ErhI CCWWGG 2 cut(s) 493, 2114
Esp3I CGTCTC 1 cut(s) 1641
FaqI GGGAC 4 cut(s) 623, 945, 1336, 1777
FauNDI CATATG 2 cut(s) 605, 2266
FbaI TGATCA 1 cut(s) 262
Fnu4HI GCNGC 6 cut(s) 32, 97, 472, 625, 1584, 2223
FokI GGATG 4 cut(s) 193, 1124, 1467, 2097
Fsp4HI GCNGC 6 cut(s) 32, 97, 472, 625, 1584, 2223
FspBI CTAG 6 cut(s) 666, 1061, 1730, 1847, 1910, 2226
GlaI GCGC 1 cut(s) 388
GluI GCNGC 6 cut(s) 32, 97, 472, 625, 1584, 2223
GsuI CTGGAG 1 cut(s) 2105
HaeII RGCGCY 1 cut(s) 390
HaeIII GGCC 5 cut(s) 5, 450, 651, 1891, 2056
HapII CCGG 4 cut(s) 93, 700, 993, 1596
HhaI GCGC 1 cut(s) 389
Hin1I GRCGYC 2 cut(s) 387, 1824
Hin6I GCGC 1 cut(s) 387
HinP1I GCGC 1 cut(s) 387
HincII GTYRAC 2 cut(s) 799, 1426
HindII GTYRAC 2 cut(s) 799, 1426
HindIII AAGCTT 2 cut(s) 84, 1915
HinfI GANTC 5 cut(s) 351, 509, 853, 1024, 1951
HpaII CCGG 4 cut(s) 93, 700, 993, 1596
HphI GGTGA 9 cut(s) 281, 1304, 1417, 1453, 1492, 1542, 1634, 1996, 2157
Hpy166II GTNNAC 6 cut(s) 799, 931, 1388, 1426, 1489, 1931
Hpy188I TCNGA 7 cut(s) 708, 717, 1035, 1702, 1772, 1838, 2202
Hpy188III TCNNGA 5 cut(s) 260, 355, 1205, 1730, 1961
Hpy8I GTNNAC 6 cut(s) 799, 931, 1388, 1426, 1489, 1931
HpyCH4III ACNGT 5 cut(s) 305, 571, 1312, 1392, 1943
HpyCH4IV ACGT 5 cut(s) 1559, 1570, 1650, 1824, 2016
HpyF10VI GCNNNNNNNGC 6 cut(s) 28, 71, 105, 447, 1592, 2018
HpyF3I CTNAG 4 cut(s) 338, 1145, 1893, 2000
HpySE526I ACGT 5 cut(s) 1559, 1570, 1650, 1824, 2016
Hsp92I GRCGYC 2 cut(s) 387, 1824
HspAI GCGC 1 cut(s) 387
KasI GGCGCC 1 cut(s) 386
KroI GCCGGC 1 cut(s) 699
KroNI GCCGGC 1 cut(s) 701
Ksp22I TGATCA 1 cut(s) 262
Kzo9I GATC 4 cut(s) 262, 1732, 2097, 2162
LmnI GCTCC 6 cut(s) 920, 1211, 1598, 1709, 1852, 2080
Lsp1109I GCAGC 6 cut(s) 43, 83, 458, 636, 1595, 2234
LweI GCATC 3 cut(s) 9, 1986, 2119
MaeI CTAG 6 cut(s) 666, 1061, 1730, 1847, 1910, 2226
MaeII ACGT 5 cut(s) 1559, 1570, 1650, 1824, 2016
MaeIII GTNAC 9 cut(s) 155, 196, 273, 416, 818, 1820, 1943, 2038, 2182
MalI GATC 4 cut(s) 264, 1734, 2099, 2164
MboI GATC 4 cut(s) 262, 1732, 2097, 2162
MhlI GDGCHC 2 cut(s) 1375, 1524
Mly113I GGCGCC 1 cut(s) 387
MlyI GAGTC 1 cut(s) 518
MmeI TCCRAC 4 cut(s) 273, 1075, 1165, 1680
MroNI GCCGGC 1 cut(s) 699
MroXI GAANNNNTTC 1 cut(s) 1791
MseI TTAA 7 cut(s) 122, 504, 542, 615, 1398, 2033, 2258
MslI CAYNNNNRTG 4 cut(s) 1434, 1558, 1628, 2246
MspA1I CMGCKG 1 cut(s) 108
MspI CCGG 4 cut(s) 93, 700, 993, 1596
MspR9I CCNGG 4 cut(s) 93, 317, 840, 939
MvaI CCWGG 3 cut(s) 317, 840, 939
MwoI GCNNNNNNNGC 6 cut(s) 28, 71, 105, 447, 1592, 2018
NaeI GCCGGC 1 cut(s) 701
NarI GGCGCC 1 cut(s) 387
NciI CCSGG 1 cut(s) 93
NcoI CCATGG 1 cut(s) 2114
NdeI CATATG 2 cut(s) 605, 2266
NdeII GATC 4 cut(s) 262, 1732, 2097, 2162
NgoMIV GCCGGC 1 cut(s) 699
NlaIV GGNNCC 4 cut(s) 388, 1725, 1782, 1907
NmuCI GTSAC 5 cut(s) 155, 196, 416, 1820, 1943
NspI RCATGY 4 cut(s) 478, 744, 795, 2245
NspV TTCGAA 3 cut(s) 435, 972, 1464
OliI CACNNNNGTG 1 cut(s) 1558
PaeI GCATGC 1 cut(s) 2245
PceI AGGCCT 2 cut(s) 1891, 2056
PciI ACATGT 2 cut(s) 740, 791
PcsI WCGNNNNNNNCGW 1 cut(s) 1203
PdiI GCCGGC 1 cut(s) 701
PdmI GAANNNNTTC 1 cut(s) 1791
PfeI GAWTC 4 cut(s) 351, 853, 1024, 1951
PkrI GCNGC 6 cut(s) 33, 98, 473, 626, 1585, 2224
PleI GAGTC 1 cut(s) 517
PluTI GGCGCC 1 cut(s) 390
PpsI GAGTC 1 cut(s) 517
Ppu21I YACGTR 1 cut(s) 1560
PscI ACATGT 2 cut(s) 740, 791
PsiI TTATAA 1 cut(s) 1317
Psp6I CCWGG 3 cut(s) 315, 838, 937
PspGI CCWGG 3 cut(s) 315, 838, 937
PspN4I GGNNCC 4 cut(s) 388, 1725, 1782, 1907
PspPI GGNCC 2 cut(s) 1780, 2198
PstI CTGCAG 3 cut(s) 626, 914, 1243
PstNI CAGNNNCTG 2 cut(s) 233, 303
RsaI GTAC 5 cut(s) 348, 461, 684, 1668, 1758
RsaNI GTAC 5 cut(s) 347, 460, 683, 1667, 1757
RseI CAYNNNNRTG 4 cut(s) 1434, 1558, 1628, 2246
SaqAI TTAA 7 cut(s) 122, 504, 542, 615, 1398, 2033, 2258
SatI GCNGC 6 cut(s) 32, 97, 472, 625, 1584, 2223
Sau3AI GATC 4 cut(s) 262, 1732, 2097, 2162
Sau96I GGNCC 2 cut(s) 1780, 2198
ScaI AGTACT 1 cut(s) 684
SchI GAGTC 1 cut(s) 518
ScrFI CCNGG 4 cut(s) 93, 317, 840, 939
SduI GDGCHC 2 cut(s) 1375, 1524
SfaNI GCATC 3 cut(s) 9, 1986, 2119
SfcI CTRYAG 4 cut(s) 622, 910, 1239, 1828
SfoI GGCGCC 1 cut(s) 388
SfuI TTCGAA 3 cut(s) 435, 972, 1464
SinI GGWCC 2 cut(s) 1780, 2198
SmiMI CAYNNNNRTG 4 cut(s) 1434, 1558, 1628, 2246
SmlI CTYRAG 3 cut(s) 355, 1250, 1419
SmoI CTYRAG 3 cut(s) 355, 1250, 1419
SphI GCATGC 1 cut(s) 2245
SseBI AGGCCT 2 cut(s) 1891, 2056
SsiI CCGC 3 cut(s) 106, 565, 1689
SspDI GGCGCC 1 cut(s) 386
SspI AATATT 3 cut(s) 54, 1233, 1990
SspMI CTAG 6 cut(s) 666, 1061, 1730, 1847, 1910, 2226
StuI AGGCCT 2 cut(s) 1891, 2056
StyD4I CCNGG 4 cut(s) 91, 315, 838, 937
StyI CCWWGG 2 cut(s) 493, 2114
TaaI ACNGT 5 cut(s) 305, 571, 1312, 1392, 1943
TaiI ACGT 5 cut(s) 1562, 1573, 1653, 1827, 2019
TaqI TCGA 5 cut(s) 435, 445, 972, 1464, 1962
TatI WGTACW 2 cut(s) 682, 1666
TfiI GAWTC 4 cut(s) 351, 853, 1024, 1951
Tru1I TTAA 7 cut(s) 122, 504, 542, 615, 1398, 2033, 2258
Tru9I TTAA 7 cut(s) 122, 504, 542, 615, 1398, 2033, 2258
TscAI CASTG 8 cut(s) 576, 919, 1375, 1479, 1938, 2107, 2152, 2224
TseFI GTSAC 5 cut(s) 155, 196, 416, 1820, 1943
TseI GCWGC 6 cut(s) 31, 96, 471, 624, 1583, 2222
Tsp45I GTSAC 5 cut(s) 155, 196, 416, 1820, 1943
TspRI CASTG 8 cut(s) 576, 919, 1375, 1479, 1938, 2107, 2152, 2224
VpaK11BI GGWCC 2 cut(s) 1780, 2198
XapI RAATTY 4 cut(s) 659, 898, 1133, 1956
XbaI TCTAGA 1 cut(s) 1729
XceI RCATGY 4 cut(s) 478, 744, 795, 2245
XmnI GAANNNNTTC 1 cut(s) 1791
XspI CTAG 6 cut(s) 666, 1061, 1730, 1847, 1910, 2226
ZraI GACGTC 1 cut(s) 1825
ZrmI AGTACT 1 cut(s) 684
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.