MD10G1250500.v1.1

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000066
Physical Location & Seq
Forward (+)
34297043 .. 34304303
7261 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1250500.v1.1.491

Sequence Viewer

Length: 2142 bp
ATGGCGTTACATAGGAGGATGCATATTTTGCATCTCTCTTTAGTAATGGTGGTAGTTGTTACAATTTTACTAGGAACTCCAACTCCAACAACAGCTCAATCTCTCCTTCCGCCTCAGCTGGCACTGCCTGACTGCCCTGACCATTGTGGCAATCTCACAATTCCATACCCATTTGGCATAGGCTCTGATTGCTACCTCGACGACCGGTTTGAAATCACTTGTAACATTTCCACTCAACCGCCAACAGCATTTTTGATGACGGGCAATGTCGAAATTACCAACATATCTCTTTATGACGGTGAGGTCCAAATACTACAGTTCGCAACCAGTGATTGTTATGATGCTCAAGGCAACTCGAACGAAACGAACAGTGCCGATCTCTCTGTGCCTCCTCCTTACACCATCTCCAAAACCAAAAACATGTTCGTTGCAGTTGGATGCGACACATATGCGTTTTTTATAGGTTACCGGGGTGAAAAAAAGTACACAACTGGGTGTATGTCCATATGTGACAATCTTGGCAACGCTATCGATCAGCGGGATACTTGCTCTGGCGTTGGATGTTGCCAAACTAAAATCCCCAGTGGGCTGCAAAATCAGACTGTGACCTTGGGAGGCTTTAACAATTATACGGATGTGCGGGACAGCAACCGATGCAGCTACGCGTTCCTTGTGCAAGAAGGCGAGTTCAACTTTAAAAATACAACTAAAAACGATAGCACTTTTGCATGCAAGGCAAATAGCTTCTGCCATAATCGGACTTCAGGCTCCATATGCCGGTGCCTGCCAGGTTACGAAGGGAACCCATACCTCCCGGATGGTTGCCAAGATATTGATGAGTGCAAGGCTCCAGACTACTGCGGTAATGGAACCTGCATAAATACACCGGGAGGTCATTCTTGTCTATGTCCGATAGGGTACAAATATGGCGATCTGGATAAAAAAACTTGCATCAAGGACAATGGTCGAGCAAAGCTCAGTCTCCTGCTTATTATTGCACTAGTCATCTTTGCAGGTGCCAGTGGAGGTTTCTTGCTTTTACTAAGTGGATCTTGGATATATTGGGGAATGCAAAGAAGAAAGTTCCTCAAACTCAAAGAAAAATACTTTAAAGAAAATGGTGGCTTATTGTTACAACAAAAAATCGCTAGTCAAGGAGGCTCTGTCGAGACAACAAAAATTTTCACGGCAGAAGAACTTGAGAAGGCAACAAACAATTACCATGAAAGTGGAATCCTTGGTGAAGGAGGCTATGGAATAGTTTACAAAGGAATATTAGCAGCAGATAACAATAAAGTGCAGTTTGTCAACGAGTTGCTTGTTCTCTCTCAAATCAACCATAGAAATGTGGTGAGGTTATTGGGTTGTTGTTTGGAGTTAGAAGTTCCTCTACTAGTTTACGAGTTTATCGCTCACGGCACTCTCTTTGAGCACATACATGGCAAAAAGAGGAAAGGATCATCATTTTCATTGGAATTACGACTCAAGATAGCTGCTGAAACTGCTGGAGCACTAGCATACTTACACTCCTCAGCTCTGATGCAAATCATACATCGAGATGTGAAACTGACAAATATACTGTTAGATGAAAATTACACGGCAAAAGTGTCAGACTTTGGAGCTTCACGATTGATTCCTCTTGATCAAGCCCAACTTGCAACTTTAGTGCAAGGAACAATCGGATACTTGGACCCTGAATACTTCCACACGAGTCAACTAACAGATAAGAGTGACGTCTATAGCTTTGGAGTTGTCCTTATGGAGCTACTAACAAGTAAACTGGCACTTGATTCTCATAGGCCTGAGGGAGAGAAAAACCTGGCAAGGTTCTTTCTTTGTTTAATGGAAGAGGATCGCCTGAATGAAATTCTCGATGATGACATACTTAACGAGAGAAACATCAAGACATTAAAAACAGTGGCCAATCTGGCAAAAAGATGTGTAAGGTTAAAAGGGGAGGACATGCCAACAATGAAAGAAGTTGCCATGGAGCTTGAAGGAATGAGAATTACAGAAAAGCATCCATGGGGAAAAGCTGAAATTTGTTCAGAAGAGACTGAGTGCTTACTTGGGCCAGGTAACTCAGATGCTTATCCTATGGATGTTAGAGCTGGTTCTAGTTCTGGTACAACTATTGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK

Protein Analysis

714

Amino Acids

78.45

Weight (kDa)

5.61

Isoelectric Point (pI)

30.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 45 - 104 1.6e-11 Wall-associated receptor kinase galacturonan-binding
EGF_CA PF07645 277 - 317 6.8e-07 Calcium-binding EGF domain
Pkinase PF00069 407 - 661 1.5e-35 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 429 - 665 1.8e-37 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21210 AT1G21230 AT1G21240 AT1G21240 AT1G21245 AT1G21250 AT1G21270 AT1G22720 AT3G25490
fragaria_vesca FvH4_2g35051 FvH4_2g35051 FvH4_2g35060 FvH4_3g10281 FvH4_3g10285 FvH4_3g10286 FvH4_3g10311 FvH4_3g10311 FvH4_3g10320 FvH4_3g10332 FvH4_3g10340 FvH4_3g10350 FvH4_3g10360 FvH4_3g10390 FvH4_3g10412 FvH4_3g10413 FvH4_3g10461 FvH4_3g10462 FvH4_3g10470 FvH4_3g10510 FvH4_3g10520 FvH4_3g10530 FvH4_3g10550 FvH4_3g10560 FvH4_3g10611 FvH4_3g11730 FvH4_6g53140 FvH4_6g53142 FvH4_7g31880 FvH4_7g31910 FvH4_7g31920
malus_domestica MD05G1269900.v1.1 MD05G1270000.v1.1 MD05G1270200.v1.1 MD05G1270400.v1.1 MD05G1270500.v1.1 MD05G1270600.v1.1 MD05G1270800.v1.1 MD05G1270900.v1.1 MD07G1183800.v1.1 MD08G1107600.v1.1 MD10G1248500.v1.1 MD10G1249200.v1.1 MD10G1249500.v1.1 MD10G1250000.v1.1 MD10G1250500.v1.1 MD10G1250900.v1.1 MD10G1251200.v1.1 MD10G1251400.v1.1 MD13G1004600.v1.1 MD15G1088400.v1.1
prunus_persica Prupe.1G442400_v2.0.a1 Prupe.1G442500_v2.0.a1 Prupe.2G223400_v2.0.a1 Prupe.4G091900_v2.0.a1 Prupe.4G093200_v2.0.a1 Prupe.4G093300_v2.0.a1 Prupe.4G093400_v2.0.a1 Prupe.4G093500_v2.0.a1 Prupe.4G094100_v2.0.a1 Prupe.4G094200_v2.0.a1 Prupe.4G094300_v2.0.a1 Prupe.6G358300_v2.0.a1
pyrus_communis pycom08g08990 pycom10g20660 pycom10g20670 pycom10g20680 pycom10g20710 pycom10g20740 pycom13g00400 pycom15g08290
rosa_chinensis RchiOBHm_Chr1g0326861 RchiOBHm_Chr1g0326871 RchiOBHm_Chr1g0331391 RchiOBHm_Chr1g0336911 RchiOBHm_Chr1g0364001 RchiOBHm_Chr2g0174541 RchiOBHm_Chr2g0175041 RchiOBHm_Chr2g0175101 RchiOBHm_Chr2g0175121 RchiOBHm_Chr3g0486331 RchiOBHm_Chr5g0015581 RchiOBHm_Chr5g0016551 RchiOBHm_Chr5g0016561 RchiOBHm_Chr5g0016581 RchiOBHm_Chr5g0016601 RchiOBHm_Chr5g0016611 RchiOBHm_Chr5g0016641 RchiOBHm_Chr5g0016661 RchiOBHm_Chr5g0016691 RchiOBHm_Chr5g0016701 RchiOBHm_Chr5g0016731 RchiOBHm_Chr5g0016741 RchiOBHm_Chr5g0016811 RchiOBHm_Chr5g0016841 RchiOBHm_Chr5g0016871 RchiOBHm_Chr5g0016881 RchiOBHm_Chr5g0016931 RchiOBHm_Chr5g0017021 RchiOBHm_Chr5g0017041 RchiOBHm_Chr5g0017071 RchiOBHm_Chr5g0017091 RchiOBHm_Chr5g0017121 RchiOBHm_Chr5g0017141 RchiOBHm_Chr5g0017291 RchiOBHm_Chr5g0017321 RchiOBHm_Chr5g0017401 RchiOBHm_Chr5g0019001 RchiOBHm_Chr5g0019071 RchiOBHm_Chr5g0028941 RchiOBHm_Chr5g0052141 RchiOBHm_Chr5g0054721 RchiOBHm_Chr5g0054801 RchiOBHm_Chr5g0054931 RchiOBHm_Chr5g0054941 RchiOBHm_Chr6g0259291 RchiOBHm_Chr6g0292991 RchiOBHm_Chr6g0306911
rosa_laevigata RLG00000009465 RLG00000009468 RLG00000010755 RLG00000011976 RLG00000014541 RLG00000014542 RLG00000015169 RLG00000022308 RLG00000027529 RLG00000029324 RLG00000030244 RLG00000030245 RLG00000032210 RLG00000032211 RLG00000032257 RLG00000032259 RLG00000032260 RLG00000032261 RLG00000032262 RLG00000032268 RLG00000032269 RLG00000032272 RLG00000032282 RLG00000032283 RLG00000032308 RLG00000032413 RLG00000033129 RLG00000034945
rosa_multiflora Rmu_co8241929.1_g000001 Rmu_co8269227.1_g000001 Rmu_co8461259.1_g000001 Rmu_co8495385.1_g000001 Rmu_sc0000028.1_g000003 Rmu_sc0000083.1_g000009 Rmu_sc0000083.1_g000010 Rmu_sc0000083.1_g000031 Rmu_sc0000083.1_g000043 Rmu_sc0000083.1_g000046 Rmu_sc0000083.1_g000047 Rmu_sc0000563.1_g000024 Rmu_sc0000842.1_g000012 Rmu_sc0001235.1_g000001 Rmu_sc0001235.1_g000005 Rmu_sc0001235.1_g000009 Rmu_sc0001235.1_g000015 Rmu_sc0001235.1_g000016 Rmu_sc0001235.1_g000017 Rmu_sc0001473.1_g000036 Rmu_sc0001607.1_g000016 Rmu_sc0001788.1_g000006 Rmu_sc0002018.1_g000008 Rmu_sc0002028.1_g000001 Rmu_sc0002680.1_g000011 Rmu_sc0002798.1_g000023 Rmu_sc0002886.1_g000023 Rmu_sc0002989.1_g000012 Rmu_sc0003064.1_g000009 Rmu_sc0003382.1_g000006 Rmu_sc0003630.1_g000002 Rmu_sc0003630.1_g000047 Rmu_sc0003630.1_g000066 Rmu_sc0003630.1_g000067 Rmu_sc0003649.1_g000010 Rmu_sc0003688.1_g000005 Rmu_sc0003920.1_g000007 Rmu_sc0004765.1_g000007 Rmu_sc0004765.1_g000008 Rmu_sc0004920.1_g000011 Rmu_sc0005223.1_g000008 Rmu_sc0005223.1_g000010 Rmu_sc0005223.1_g000015 Rmu_sc0005223.1_g000016 Rmu_sc0005319.1_g000014 Rmu_sc0005319.1_g000015 Rmu_sc0005319.1_g000018 Rmu_sc0005319.1_g000019 Rmu_sc0005319.1_g000025 Rmu_sc0005418.1_g000007 Rmu_sc0005418.1_g000012 Rmu_sc0006106.1_g000011 Rmu_sc0006914.1_g000012 Rmu_sc0007023.1_g000001 Rmu_sc0007662.1_g000001 Rmu_sc0007920.1_g000002 Rmu_sc0007920.1_g000013 Rmu_sc0008053.1_g000016 Rmu_sc0008053.1_g000017 Rmu_sc0008053.1_g000018 Rmu_sc0008053.1_g000023 Rmu_sc0008053.1_g000050 Rmu_sc0009042.1_g000007 Rmu_sc0009042.1_g000008 Rmu_sc0010642.1_g000008 Rmu_sc0011109.1_g000002 Rmu_sc0011314.1_g000006 Rmu_sc0011974.1_g000006 Rmu_sc0012179.1_g000009 Rmu_sc0015169.1_g000001 Rmu_sc0016368.1_g000002 Rmu_sc0022659.1_g000001 Rmu_sc0027371.1_g000001 Rmu_sc0028213.1_g000001 Rmu_sc0034526.1_g000001 Rmu_sc0039366.1_g000002 Rmu_ssc0000050.1_g000084 Rmu_ssc0000050.1_g000088 Rmu_ssc0000167.1_g000017
rosa_roxburghii Rroxscaffold_1G00011340 Rroxscaffold_1G00025710 Rroxscaffold_1G00025870 Rroxscaffold_1G00026070 Rroxscaffold_1G00026170 Rroxscaffold_1G00026200 Rroxscaffold_1G00026270 Rroxscaffold_1G00050950 Rroxscaffold_1G00058900 Rroxscaffold_1G00060040 Rroxscaffold_1G00060060 Rroxscaffold_1G00060070 Rroxscaffold_1G00060080 Rroxscaffold_1G00060110 Rroxscaffold_1G00060120 Rroxscaffold_1G00060140 Rroxscaffold_1G00060170 Rroxscaffold_1G00060210 Rroxscaffold_1G00060220 Rroxscaffold_1G00060240 Rroxscaffold_1G00060250 Rroxscaffold_1G00060260 Rroxscaffold_1G00060300 Rroxscaffold_1G00060320 Rroxscaffold_1G00060330 Rroxscaffold_1G00060900 Rroxscaffold_1G00060920 Rroxscaffold_1G00073980 Rroxscaffold_2G00077560 Rroxscaffold_2G00077580 Rroxscaffold_2G00146990 Rroxscaffold_3G00236200 Rroxscaffold_3G00236210 Rroxscaffold_3G00236220 Rroxscaffold_4G00292760 Rroxscaffold_4G00315200 Rroxscaffold_4G00315240 Rroxscaffold_4G00326240 Rroxscaffold_4G00328980 Rroxscaffold_5G00341890 Rroxscaffold_7G00161350 Rroxscaffold_7G00174490 Rroxscaffold_7G00206550
rosa_rugosa Rorug01G0093000 Rorug01G0093100 Rorug01G0128800 Rorug01G0312000 Rorug01G0312000 Rorug02G0581100 Rorug03G0224600 Rorug05G0028500 Rorug05G0032700 Rorug05G0032800 Rorug05G0032900 Rorug05G0032900 Rorug05G0033000 Rorug05G0033100 Rorug05G0033300 Rorug05G0033400 Rorug05G0033900 Rorug05G0034900 Rorug05G0035000 Rorug05G0046900 Rorug05G0047500 Rorug05G0047500 Rorug05G0111600 Rorug05G0287900 Rorug05G0288700 Rorug05G0586900 Rorug06G0231700 Rorug06G0354300 Rorug06G0354400
rosa_samantha Rh1DG122200 Rh2DG686800 Rh5BG136600 Rh6AG344500 Rh6BG351900 Rh6CG359000 Rh6CG359100 Rh6CG387600 Rh6CG480500 Rh6DG344600 Rh6DG467400
rosa_wichuraiana Rw0G010250 Rw0G014820 Rw0G021400 Rw0G023100 Rw1G009450 Rw1G012100 Rw1G012180 Rw1G028360 Rw2G054260 Rw3G024370 Rw3G024420 Rw3G024470 Rw5G010850 Rw5G011010 Rw5G011020 Rw5G011050 Rw5G011080 Rw5G011120 Rw5G012300 Rw5G018470 Rw5G033830 Rw6G008930 Rw6G030070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1004
AasI GACNNNNNNGTC 1 cut(s) 302
AatII GACGTC 1 cut(s) 1737
Acc36I ACCTGC 2 cut(s) 881, 1004
AccB1I GGYRCC 2 cut(s) 780, 1016
AccII CGCG 1 cut(s) 665
AciI CCGC 5 cut(s) 110, 239, 538, 640, 861
AclWI GGATC 3 cut(s) 1057, 1465, 1860
AcoI YGGCCR 1 cut(s) 1920
AcsI RAATTY 3 cut(s) 1179, 1866, 2040
AcuI CTGAAG 1 cut(s) 747
AcyI GRCGYC 1 cut(s) 1734
AfaI GTAC 3 cut(s) 485, 920, 2128
AfiI CCNNNNNNNGG 1 cut(s) 777
AflIII ACRYGT 2 cut(s) 420, 663
AgeI ACCGGT 1 cut(s) 204
AgsI TTSAA 3 cut(s) 212, 691, 1997
AhlI ACTAGT 2 cut(s) 1000, 1393
AjnI CCWGG 3 cut(s) 787, 1818, 2074
AjuI GAANNNNNNNTTGG 2 cut(s) 407, 439
AloI GAACNNNNNNTCC 2 cut(s) 67, 99
Alw21I GWGCWC 2 cut(s) 1434, 1513
Alw26I GTCTC 3 cut(s) 986, 1163, 2048
AlwI GGATC 3 cut(s) 1057, 1465, 1860
AoxI GGCC 3 cut(s) 1799, 1920, 2072
ApeKI GCWGC 4 cut(s) 589, 657, 1280, 1493
ApoI RAATTY 3 cut(s) 1179, 1866, 2040
ArsI GACNNNNNNTTYG 2 cut(s) 191, 223
AsiGI ACCGGT 1 cut(s) 204
AspS9I GGNCC 3 cut(s) 304, 1690, 2072
AsuC2I CCSGG 3 cut(s) 470, 815, 888
AsuHPI GGTGA 4 cut(s) 311, 485, 1253, 1363
AvaII GGWCC 2 cut(s) 304, 1690
AxyI CCTNAGG 1 cut(s) 1803
BalI TGGCCA 1 cut(s) 1922
BanI GGYRCC 2 cut(s) 780, 1016
BarI GAAGNNNNNNTAC 2 cut(s) 1374, 1406
BauI CACGAG 1 cut(s) 1708
Bbv12I GWGCWC 2 cut(s) 1434, 1513
BbvCI CCTCAGC 2 cut(s) 114, 1531
BbvI GCAGC 4 cut(s) 576, 669, 1292, 1480
BccI CCATC 2 cut(s) 410, 812
BceAI ACGGC 3 cut(s) 1203, 1432, 1614
BcgI CGANNNNNNTGC 4 cut(s) 431, 465, 511, 545
BciT130I CCWGG 3 cut(s) 789, 1820, 2076
BciVI GTATCC 2 cut(s) 535, 1676
BclI TGATCA 1 cut(s) 1642
BcnI CCSGG 3 cut(s) 470, 815, 888
BcoDI GTCTC 3 cut(s) 986, 1163, 2048
BcuI ACTAGT 2 cut(s) 1000, 1393
BfaI CTAG 6 cut(s) 71, 1001, 1149, 1394, 1514, 2118
BfmI CTRYAG 2 cut(s) 314, 1738
BfuAI ACCTGC 2 cut(s) 881, 1004
BfuI GTATCC 2 cut(s) 535, 1676
BglI GCCNNNNNGGC 1 cut(s) 1928
BisI GCNGC 4 cut(s) 590, 658, 1281, 1494
BlsI GCNGC 4 cut(s) 591, 659, 1282, 1495
Bme1390I CCNGG 6 cut(s) 470, 789, 815, 888, 1820, 2076
Bme18I GGWCC 2 cut(s) 304, 1690
BmgT120I GGNCC 3 cut(s) 304, 1690, 2072
BmiI GGNNCC 7 cut(s) 769, 782, 803, 849, 871, 1018, 1692
BmrFI CCNGG 6 cut(s) 470, 789, 815, 888, 1820, 2076
BmrI ACTGGG 2 cut(s) 501, 576
BmsI GCATC 9 cut(s) 9, 40, 331, 428, 644, 960, 1530, 2029, 2077
BmuI ACTGGG 2 cut(s) 501, 576
BoxI GACNNNNGTC 1 cut(s) 963
BplI GAGNNNNNCTC 2 cut(s) 960, 992
BpmI CTGGAG 2 cut(s) 834, 1527
Bpu10I CCTNAGC 2 cut(s) 114, 1531
BpuEI CTTGAG 3 cut(s) 330, 1220, 1469
BpuMI CCSGG 3 cut(s) 470, 815, 888
Bsa29I ATCGAT 1 cut(s) 531
BsaBI GATNNNNATC 2 cut(s) 1544, 2091
BsaHI GRCGYC 1 cut(s) 1734
BsaJI CCNNGG 5 cut(s) 469, 609, 1237, 1986, 2024
BsaWI WCCGGW 1 cut(s) 204
BsaXI ACNNNNNCTCC 8 cut(s) 67, 97, 389, 419, 1149, 1179, 1511, 1541
Bsc4I CCNNNNNNNGG 1 cut(s) 777
Bse118I RCCGGY 2 cut(s) 204, 777
Bse1I ACTGG 5 cut(s) 327, 496, 582, 1020, 1785
Bse21I CCTNAGG 1 cut(s) 1803
Bse3DI GCAATG 1 cut(s) 271
Bse8I GATNNNNATC 2 cut(s) 1544, 2091
BseBI CCWGG 3 cut(s) 789, 1820, 2076
BseCI ATCGAT 1 cut(s) 531
BseDI CCNNGG 5 cut(s) 469, 609, 1237, 1986, 2024
BseGI GGATG 7 cut(s) 24, 443, 566, 640, 823, 2020, 2107
BseJI GATNNNNATC 2 cut(s) 1544, 2091
BseLI CCNNNNNNNGG 1 cut(s) 777
BseMI GCAATG 1 cut(s) 271
BseMII CTCAG 6 cut(s) 128, 991, 1545, 1794, 2049, 2097
BseNI ACTGG 5 cut(s) 327, 496, 582, 1020, 1785
BseRI GAGGAG 2 cut(s) 381, 1519
BseXI GCAGC 4 cut(s) 576, 669, 1292, 1480
BsgI GTGCAG 1 cut(s) 1319
Bsh1236I CGCG 1 cut(s) 665
Bsh1285I CGRYCG 1 cut(s) 205
BshFI GGCC 3 cut(s) 1801, 1922, 2074
BshNI GGYRCC 2 cut(s) 780, 1016
BshTI ACCGGT 1 cut(s) 204
BshVI ATCGAT 1 cut(s) 531
BsiEI CGRYCG 1 cut(s) 205
BsiHKAI GWGCWC 2 cut(s) 1434, 1513
BsiSI CCGG 5 cut(s) 205, 469, 778, 815, 887
BslFI GGGAC 1 cut(s) 656
BslI CCNNNNNNNGG 1 cut(s) 777
BsmAI GTCTC 3 cut(s) 986, 1163, 2048
BsmFI GGGAC 1 cut(s) 656
BsmI GAATGC 1 cut(s) 1074
BsnI GGCC 3 cut(s) 1801, 1922, 2074
Bsp1286I GDGCHC 2 cut(s) 1434, 1513
Bsp143I GATC 7 cut(s) 376, 532, 931, 1049, 1457, 1642, 1852
Bsp19I CCATGG 2 cut(s) 1986, 2024
BspACI CCGC 5 cut(s) 110, 239, 538, 640, 861
BspANI GGCC 3 cut(s) 1801, 1922, 2074
BspCNI CTCAG 6 cut(s) 127, 990, 1544, 1795, 2050, 2096
BspDI ATCGAT 1 cut(s) 531
BspFNI CGCG 1 cut(s) 665
BspLI GGNNCC 7 cut(s) 769, 782, 803, 849, 871, 1018, 1692
BspMI ACCTGC 2 cut(s) 881, 1004
BspPI GGATC 3 cut(s) 1057, 1465, 1860
BspT107I GGYRCC 2 cut(s) 780, 1016
BsrDI GCAATG 1 cut(s) 271
BsrFI RCCGGY 2 cut(s) 204, 777
BsrI ACTGG 5 cut(s) 327, 496, 582, 1020, 1785
BssAI RCCGGY 2 cut(s) 204, 777
BssECI CCNNGG 5 cut(s) 469, 609, 1237, 1986, 2024
BssMI GATC 7 cut(s) 376, 532, 931, 1049, 1457, 1642, 1852
BssNI GRCGYC 1 cut(s) 1734
BssSI CACGAG 1 cut(s) 1708
BssT1I CCWWGG 4 cut(s) 609, 1237, 1986, 2024
Bst2BI CACGAG 1 cut(s) 1708
Bst2UI CCWGG 3 cut(s) 789, 1820, 2076
Bst4CI ACNGT 6 cut(s) 299, 318, 371, 604, 1581, 1918
Bst6I CTCTTC 2 cut(s) 1842, 2046
BstACI GRCGYC 1 cut(s) 1734
BstAPI GCANNNNNTGC 2 cut(s) 28, 654
BstC8I GCNNGC 3 cut(s) 120, 730, 785
BstDEI CTNAG 7 cut(s) 114, 977, 1043, 1531, 1803, 2058, 2083
BstDSI CCRYGG 2 cut(s) 1986, 2024
BstEII GGTNACC 1 cut(s) 464
BstF5I GGATG 7 cut(s) 24, 443, 566, 640, 823, 2020, 2107
BstFNI CGCG 1 cut(s) 665
BstKTI GATC 7 cut(s) 379, 535, 934, 1052, 1460, 1645, 1855
BstMAI GTCTC 3 cut(s) 986, 1163, 2048
BstMBI GATC 7 cut(s) 376, 532, 931, 1049, 1457, 1642, 1852
BstMCI CGRYCG 1 cut(s) 205
BstMWI GCNNNNNNNGC 9 cut(s) 28, 124, 189, 654, 734, 774, 1502, 1655, 1928
BstNI CCWGG 3 cut(s) 789, 1820, 2076
BstNSI RCATGY 3 cut(s) 424, 732, 1966
BstPAI GACNNNNGTC 1 cut(s) 963
BstPI GGTNACC 1 cut(s) 464
BstSCI CCNGG 6 cut(s) 468, 787, 813, 886, 1818, 2074
BstSFI CTRYAG 2 cut(s) 314, 1738
BstUI CGCG 1 cut(s) 665
BstV1I GCAGC 4 cut(s) 576, 669, 1292, 1480
BstX2I RGATCY 1 cut(s) 1049
BstXI CCANNNNNNTGG 1 cut(s) 1229
BstYI RGATCY 1 cut(s) 1049
Bsu15I ATCGAT 1 cut(s) 531
Bsu36I CCTNAGG 1 cut(s) 1803
BsuI GTATCC 2 cut(s) 535, 1676
BsuRI GGCC 3 cut(s) 1801, 1922, 2074
BsuTUI ATCGAT 1 cut(s) 531
BtgI CCRYGG 2 cut(s) 1986, 2024
BtsCI GGATG 7 cut(s) 24, 443, 566, 640, 823, 2020, 2107
BtsI GCAGTG 1 cut(s) 122
BtsIMutI CAGTG 6 cut(s) 122, 334, 376, 589, 1027, 1923
BveI ACCTGC 2 cut(s) 881, 1004
Cac8I GCNNGC 3 cut(s) 120, 730, 785
Cfr10I RCCGGY 2 cut(s) 204, 777
Cfr13I GGNCC 3 cut(s) 304, 1690, 2072
ClaI ATCGAT 1 cut(s) 531
Csp6I GTAC 3 cut(s) 484, 919, 2127
CspAI ACCGGT 1 cut(s) 204
CviAII CATG 7 cut(s) 421, 729, 1223, 1439, 1963, 1987, 2025
CviQI GTAC 3 cut(s) 484, 919, 2127
DdeI CTNAG 7 cut(s) 114, 977, 1043, 1531, 1803, 2058, 2083
DpnI GATC 7 cut(s) 378, 534, 933, 1051, 1459, 1644, 1854
DpnII GATC 7 cut(s) 376, 532, 931, 1049, 1457, 1642, 1852
DraI TTTAAA 2 cut(s) 697, 1111
DrdI GACNNNNNNGTC 1 cut(s) 302
DseDI GACNNNNNNGTC 1 cut(s) 302
EaeI YGGCCR 1 cut(s) 1920
Eam1104I CTCTTC 2 cut(s) 1842, 2046
EarI CTCTTC 2 cut(s) 1842, 2046
EciI GGCGGA 1 cut(s) 99
Eco130I CCWWGG 4 cut(s) 609, 1237, 1986, 2024
Eco147I AGGCCT 1 cut(s) 1801
Eco47I GGWCC 2 cut(s) 304, 1690
Eco57I CTGAAG 1 cut(s) 747
Eco81I CCTNAGG 1 cut(s) 1803
Eco91I GGTNACC 1 cut(s) 464
EcoO65I GGTNACC 1 cut(s) 464
EcoRII CCWGG 3 cut(s) 787, 1818, 2074
EcoT14I CCWWGG 4 cut(s) 609, 1237, 1986, 2024
EcoT22I ATGCAT 1 cut(s) 24
ErhI CCWWGG 4 cut(s) 609, 1237, 1986, 2024
FaeI CATG 7 cut(s) 424, 732, 1226, 1442, 1966, 1990, 2028
FalI AAGNNNNNCTT 4 cut(s) 1036, 1068, 1638, 1670
FaqI GGGAC 1 cut(s) 656
FatI CATG 7 cut(s) 420, 728, 1222, 1438, 1962, 1986, 2024
FauI CCCGC 2 cut(s) 531, 633
FauNDI CATATG 3 cut(s) 448, 506, 773
FbaI TGATCA 1 cut(s) 1642
Fnu4HI GCNGC 4 cut(s) 590, 658, 1281, 1494
FokI GGATG 7 cut(s) 31, 450, 573, 647, 830, 2007, 2114
Fsp4HI GCNGC 4 cut(s) 590, 658, 1281, 1494
FspBI CTAG 6 cut(s) 71, 1001, 1149, 1394, 1514, 2118
GluI GCNGC 4 cut(s) 590, 658, 1281, 1494
GsuI CTGGAG 2 cut(s) 834, 1527
HaeIII GGCC 3 cut(s) 1801, 1922, 2074
HapII CCGG 5 cut(s) 205, 469, 778, 815, 887
Hin1I GRCGYC 1 cut(s) 1734
Hin1II CATG 7 cut(s) 424, 732, 1226, 1442, 1966, 1990, 2028
HincII GTYRAC 2 cut(s) 1309, 1715
HindII GTYRAC 2 cut(s) 1309, 1715
HinfI GANTC 5 cut(s) 1233, 1482, 1633, 1711, 1790
HpaII CCGG 5 cut(s) 205, 469, 778, 815, 887
HphI GGTGA 4 cut(s) 311, 485, 1253, 1363
Hpy166II GTNNAC 6 cut(s) 486, 1264, 1309, 1399, 1715, 1778
Hpy188I TCNGA 9 cut(s) 187, 600, 759, 912, 1539, 1612, 1682, 2050, 2086
Hpy188III TCNNGA 9 cut(s) 851, 935, 1168, 1486, 1556, 1626, 1640, 1871, 1903
Hpy8I GTNNAC 6 cut(s) 486, 1264, 1309, 1399, 1715, 1778
Hpy99I CGWCG 1 cut(s) 203
HpyAV CCTTC 6 cut(s) 116, 674, 791, 1198, 1238, 1991
HpyCH4III ACNGT 6 cut(s) 299, 318, 371, 604, 1581, 1918
HpyCH4IV ACGT 1 cut(s) 1734
HpyF10VI GCNNNNNNNGC 9 cut(s) 28, 124, 189, 654, 734, 774, 1502, 1655, 1928
HpyF3I CTNAG 7 cut(s) 114, 977, 1043, 1531, 1803, 2058, 2083
HpySE526I ACGT 1 cut(s) 1734
Hsp92I GRCGYC 1 cut(s) 1734
Hsp92II CATG 7 cut(s) 424, 732, 1226, 1442, 1966, 1990, 2028
Ksp22I TGATCA 1 cut(s) 1642
Kzo9I GATC 7 cut(s) 376, 532, 931, 1049, 1457, 1642, 1852
LmnI GCTCC 6 cut(s) 773, 853, 1508, 1619, 1762, 1990
Lsp1109I GCAGC 4 cut(s) 576, 669, 1292, 1480
LweI GCATC 9 cut(s) 9, 40, 331, 428, 644, 960, 1530, 2029, 2077
MaeI CTAG 6 cut(s) 71, 1001, 1149, 1394, 1514, 2118
MaeII ACGT 1 cut(s) 1734
MalI GATC 7 cut(s) 378, 534, 933, 1051, 1459, 1644, 1854
MboI GATC 7 cut(s) 376, 532, 931, 1049, 1457, 1642, 1852
MboII GAAGA 4 cut(s) 1089, 1205, 1859, 2063
MflI RGATCY 1 cut(s) 1049
MhlI GDGCHC 2 cut(s) 1434, 1513
MlsI TGGCCA 1 cut(s) 1922
MluI ACGCGT 1 cut(s) 663
MluNI TGGCCA 1 cut(s) 1922
MlyI GAGTC 2 cut(s) 1476, 1720
MmeI TCCRAC 4 cut(s) 104, 110, 415, 538
Mox20I TGGCCA 1 cut(s) 1922
Mph1103I ATGCAT 1 cut(s) 24
MscI TGGCCA 1 cut(s) 1922
MseI TTAA 7 cut(s) 621, 696, 1110, 1841, 1887, 1910, 1949
MslI CAYNNNNRTG 4 cut(s) 1227, 1344, 1437, 1557
Msp20I TGGCCA 1 cut(s) 1922
MspA1I CMGCKG 2 cut(s) 118, 538
MspI CCGG 5 cut(s) 205, 469, 778, 815, 887
MspR9I CCNGG 6 cut(s) 470, 789, 815, 888, 1820, 2076
Mva1269I GAATGC 1 cut(s) 1074
MvaI CCWGG 3 cut(s) 789, 1820, 2076
MvnI CGCG 1 cut(s) 665
MwoI GCNNNNNNNGC 9 cut(s) 28, 124, 189, 654, 734, 774, 1502, 1655, 1928
NciI CCSGG 3 cut(s) 470, 815, 888
NcoI CCATGG 2 cut(s) 1986, 2024
NdeI CATATG 3 cut(s) 448, 506, 773
NdeII GATC 7 cut(s) 376, 532, 931, 1049, 1457, 1642, 1852
NlaIII CATG 7 cut(s) 424, 732, 1226, 1442, 1966, 1990, 2028
NlaIV GGNNCC 7 cut(s) 769, 782, 803, 849, 871, 1018, 1692
NmuCI GTSAC 3 cut(s) 509, 604, 1730
NsiI ATGCAT 1 cut(s) 24
NspI RCATGY 3 cut(s) 424, 732, 1966
PaeI GCATGC 1 cut(s) 732
PaqCI CACCTGC 1 cut(s) 1004
PceI AGGCCT 1 cut(s) 1801
PciI ACATGT 1 cut(s) 420
PcsI WCGNNNNNNNCGW 1 cut(s) 362
PctI GAATGC 1 cut(s) 1074
PfeI GAWTC 3 cut(s) 1233, 1633, 1790
PfoI TCCNGGA 1 cut(s) 813
PinAI ACCGGT 1 cut(s) 204
PkrI GCNGC 4 cut(s) 591, 659, 1282, 1495
PleI GAGTC 2 cut(s) 1476, 1719
PpsI GAGTC 2 cut(s) 1476, 1719
PscI ACATGT 1 cut(s) 420
PshAI GACNNNNGTC 1 cut(s) 963
Psp6I CCWGG 3 cut(s) 787, 1818, 2074
PspEI GGTNACC 1 cut(s) 464
PspGI CCWGG 3 cut(s) 787, 1818, 2074
PspN4I GGNNCC 7 cut(s) 769, 782, 803, 849, 871, 1018, 1692
PspPI GGNCC 3 cut(s) 304, 1690, 2072
PsuI RGATCY 1 cut(s) 1049
PvuII CAGCTG 1 cut(s) 118
RsaI GTAC 3 cut(s) 485, 920, 2128
RsaNI GTAC 3 cut(s) 484, 919, 2127
RseI CAYNNNNRTG 4 cut(s) 1227, 1344, 1437, 1557
SaqAI TTAA 7 cut(s) 621, 696, 1110, 1841, 1887, 1910, 1949
SatI GCNGC 4 cut(s) 590, 658, 1281, 1494
Sau3AI GATC 7 cut(s) 376, 532, 931, 1049, 1457, 1642, 1852
Sau96I GGNCC 3 cut(s) 304, 1690, 2072
SchI GAGTC 2 cut(s) 1476, 1720
ScrFI CCNGG 6 cut(s) 470, 789, 815, 888, 1820, 2076
SduI GDGCHC 2 cut(s) 1434, 1513
SfaNI GCATC 9 cut(s) 9, 40, 331, 428, 644, 960, 1530, 2029, 2077
SfcI CTRYAG 2 cut(s) 314, 1738
SinI GGWCC 2 cut(s) 304, 1690
SmiMI CAYNNNNRTG 4 cut(s) 1227, 1344, 1437, 1557
SmlI CTYRAG 3 cut(s) 345, 1199, 1484
SmoI CTYRAG 3 cut(s) 345, 1199, 1484
SpeI ACTAGT 2 cut(s) 1000, 1393
SphI GCATGC 1 cut(s) 732
SseBI AGGCCT 1 cut(s) 1801
SsiI CCGC 5 cut(s) 110, 239, 538, 640, 861
SspI AATATT 1 cut(s) 1275
SspMI CTAG 6 cut(s) 71, 1001, 1149, 1394, 1514, 2118
StuI AGGCCT 1 cut(s) 1801
StyD4I CCNGG 6 cut(s) 468, 787, 813, 886, 1818, 2074
StyI CCWWGG 4 cut(s) 609, 1237, 1986, 2024
TaaI ACNGT 6 cut(s) 299, 318, 371, 604, 1581, 1918
TaiI ACGT 1 cut(s) 1737
TaqI TCGA 8 cut(s) 198, 270, 356, 531, 967, 1167, 1555, 1872
TatI WGTACW 1 cut(s) 483
TfiI GAWTC 3 cut(s) 1233, 1633, 1790
Tru1I TTAA 7 cut(s) 621, 696, 1110, 1841, 1887, 1910, 1949
Tru9I TTAA 7 cut(s) 621, 696, 1110, 1841, 1887, 1910, 1949
TscAI CASTG 6 cut(s) 129, 334, 376, 589, 1027, 1923
TseFI GTSAC 3 cut(s) 509, 604, 1730
TseI GCWGC 4 cut(s) 589, 657, 1280, 1493
Tsp45I GTSAC 3 cut(s) 509, 604, 1730
TspDTI ATGAA 5 cut(s) 1239, 1458, 1602, 1878, 1988
TspGWI ACGGA 1 cut(s) 647
TspRI CASTG 6 cut(s) 129, 334, 376, 589, 1027, 1923
VpaK11BI GGWCC 2 cut(s) 304, 1690
XapI RAATTY 3 cut(s) 1179, 1866, 2040
XceI RCATGY 3 cut(s) 424, 732, 1966
XspI CTAG 6 cut(s) 71, 1001, 1149, 1394, 1514, 2118
ZraI GACGTC 1 cut(s) 1735
Zsp2I ATGCAT 1 cut(s) 24
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.