Rroxscaffold_1G00060920

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000066
Physical Location & Seq
Reverse (-)
83136450 .. 83139309
2860 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00060920.1

Sequence Viewer

Length: 2235 bp
ATGGCGTTGCGACTCTCTTTGGTGGCAGTCCTACTCCTGTTATCGGCGGCTAGTACTACTACGACAACAGCAGCTCCTGCTCAAGCCCTGCCTGGCTGCTCTGACAAATGCGGCAATCTCACAATTCCATATCCATTTGGCATGGAGAAGAATTGTTCCCTGGTGGACCGCTTCTTCATCGAGTGTCGCAGGGGAATAGCATACTTGGGCAACGAATCCACCGGTATCATTGTTACTAAAATATCCATAGACGTTGGGGAGTTGCAAATATGGAACTATGTAGGGCGAGATTGTTACGATCAAGAGGGCAATGGAACTAACAGAAGCAATATGCCAGAGCTCTGGTTGGCACCACCGTACACCATATCCGATATCAAAAACAAGTTCTACGCCGTTGGTTGTGACACGTACGCAGTCATGCATGGCTTCCGAGGGGAAGATGAATTCCTTACTGGGTGCGTGTCAGTATGCTACAGCCTTGGCAGTGTTGATAAGAACTCTTGCTCTGGCGTTGGGTGTTGCCAGACTAACATCCCCAGTGGACTGCACAATCGTACTGTGACTTTTATGAGCTACTACAAGCATTCGTATGTGTGGGACTTTAACCCCTGCAGCTACGCCTTCATTGCGGAACAAGGCCAGTTCAATTTCTCCCCTGCAAGTTTTGAACAACTGAATGGCACTGAACAGCTTCCAATGGTTCTTAATTGGGCCGTTGGGAATGACAATGACCCCTGTGATGAAGCTCAAAAGAGGAAGGATTTCGCTTGCAAGGCTCATAGCAACTGCGTCAACAGGCCTATCAATGGGTCTACAGGTTACCTGTGCCAGTGCTGGCCTGGTTACGAAGGAAACCCCTACCATCCAGATGGTTGCCAAGATATTGATGAGTGCAAGGCTCCAAACCTCTGCCAGCATGGAAAGTGCTCAAATTCACCAGGAAATTACTCTTGTTCATGTCACAAAGGTTACAAAAACGACCGCATGAATGACAAAATTTGCGTGAAGGACGATCCCGTGCTTATTGTTGTATTGGGTGTAAGTGTAGGCTTCTTGGTTTTATTCGTTGGAATTTCATGGTTATGGTGGGGAAATAAGAAAAGACAGTTCATCAAATTGAAAGAAAAGTACTTCAAAGAAAATGGAGGCGTATTATTACAACAACAACTTGCCAGTCACGGAGGTTCCGTGGAAACAACAAAAATTTTTAGTGCAGATGCACTTCACAAGGCTACAAACAATTACCATGAAAGTAGAGTTCTTGGCAAAGGAGGCTATGGAATAGTATACAAAGGTATACTACCAGATAACCAAGTGATTGCCATAAAGAAGTCAAAAGTTTGTGCCGGCAGTCAGAGTGAGCAATTTGTCAACGAGGTAATCATTCTTTCTCAAATCAACCACAAAAATGTTGTGAAGCTTTTAGGTTGTTGTTTAGAAACAGAAGTGCCCATACTTGTATACGAGTTCATCAGCCATGGCACTCTTCATGAGCACATTCATAAGAAAAGATCATCACTCTCATTTGAATTACGAATGAAGATAGCAGCTGAAGCAGCAGGAGCACTAGCCCATTTGCACTCCTCAATTTCCACACCAATCATACATCGAGATGTGAAAGCAGCAAATATTCTGTTAGATGATAATTATATGGCGAAAGTGTCAGACTTTGGAGCTTCACGATTGATTTCTTCAGGTGAAGCTGGAATCCAAACTGTAGTGCTCGGGACAATCGGATACCTGGACCCTGAATATCTGCATTCAAACCAACTAACAGAAAAGAGTGACGTTTACAGCTTTGGGGTTGTCTTGGTTGAGCTTTTAACAAGCAAAGTGGCAGTTTCTAGAGATAATAGATGCTTAGCAAGCATCTTTATTGCTTCCATGGAAGAAGATTGCTTAAATCAAGTGCTTGATGATAAGATAGTGAATGAAGCAAATATTGAGATGGTGAAAAATGTGGCCACTCTTGCAAGAAGATGTTTGAGGGTAAAAGGCGAGGAAAGGCCTACCATGAGAGAAGTAGCCATGGAGCTAGAAGGAATGATTATGACAAAGCATTTATGGGGTTCTGCAGATCATTACTTCCCAGAAGAGACTGAGCACTTGCTTGGATCACCTAATACTTCCAAGGATTATGCTGCGAATGTTGATGGTGGTGGTGGTCCTGGTACTGCCAGTGGGTCTAGCATGCAAATAGACATGTCAATGTCTTCATATGCTGATGGACGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK

Protein Analysis

744

Amino Acids

81.76

Weight (kDa)

6.01

Isoelectric Point (pI)

37.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 33 - 89 2.1e-07 Wall-associated receptor kinase galacturonan-binding
EGF_CA PF07645 294 - 329 1.6e-06 Calcium-binding EGF domain
Pkinase PF00069 415 - 675 4.3e-49 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 418 - 679 8.9e-50 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21210 AT1G21230 AT1G21240 AT1G21240 AT1G21245 AT1G21250 AT1G21270 AT1G22720 AT3G25490
fragaria_vesca FvH4_2g35051 FvH4_2g35051 FvH4_2g35060 FvH4_3g10281 FvH4_3g10285 FvH4_3g10286 FvH4_3g10311 FvH4_3g10311 FvH4_3g10320 FvH4_3g10332 FvH4_3g10340 FvH4_3g10350 FvH4_3g10360 FvH4_3g10390 FvH4_3g10412 FvH4_3g10413 FvH4_3g10461 FvH4_3g10462 FvH4_3g10470 FvH4_3g10510 FvH4_3g10520 FvH4_3g10530 FvH4_3g10550 FvH4_3g10560 FvH4_3g10611 FvH4_3g11730 FvH4_6g53140 FvH4_6g53142 FvH4_7g31880 FvH4_7g31910 FvH4_7g31920
malus_domestica MD05G1269900.v1.1 MD05G1270000.v1.1 MD05G1270200.v1.1 MD05G1270400.v1.1 MD05G1270500.v1.1 MD05G1270600.v1.1 MD05G1270800.v1.1 MD05G1270900.v1.1 MD07G1183800.v1.1 MD08G1107600.v1.1 MD10G1248500.v1.1 MD10G1249200.v1.1 MD10G1249500.v1.1 MD10G1250000.v1.1 MD10G1250500.v1.1 MD10G1250900.v1.1 MD10G1251200.v1.1 MD10G1251400.v1.1 MD13G1004600.v1.1 MD15G1088400.v1.1
prunus_persica Prupe.1G442400_v2.0.a1 Prupe.1G442500_v2.0.a1 Prupe.2G223400_v2.0.a1 Prupe.4G091900_v2.0.a1 Prupe.4G093200_v2.0.a1 Prupe.4G093300_v2.0.a1 Prupe.4G093400_v2.0.a1 Prupe.4G093500_v2.0.a1 Prupe.4G094100_v2.0.a1 Prupe.4G094200_v2.0.a1 Prupe.4G094300_v2.0.a1 Prupe.6G358300_v2.0.a1
pyrus_communis pycom08g08990 pycom10g20660 pycom10g20670 pycom10g20680 pycom10g20710 pycom10g20740 pycom13g00400 pycom15g08290
rosa_chinensis RchiOBHm_Chr1g0326861 RchiOBHm_Chr1g0326871 RchiOBHm_Chr1g0331391 RchiOBHm_Chr1g0336911 RchiOBHm_Chr1g0364001 RchiOBHm_Chr2g0174541 RchiOBHm_Chr2g0175041 RchiOBHm_Chr2g0175101 RchiOBHm_Chr2g0175121 RchiOBHm_Chr3g0486331 RchiOBHm_Chr5g0015581 RchiOBHm_Chr5g0016551 RchiOBHm_Chr5g0016561 RchiOBHm_Chr5g0016581 RchiOBHm_Chr5g0016601 RchiOBHm_Chr5g0016611 RchiOBHm_Chr5g0016641 RchiOBHm_Chr5g0016661 RchiOBHm_Chr5g0016691 RchiOBHm_Chr5g0016701 RchiOBHm_Chr5g0016731 RchiOBHm_Chr5g0016741 RchiOBHm_Chr5g0016811 RchiOBHm_Chr5g0016841 RchiOBHm_Chr5g0016871 RchiOBHm_Chr5g0016881 RchiOBHm_Chr5g0016931 RchiOBHm_Chr5g0017021 RchiOBHm_Chr5g0017041 RchiOBHm_Chr5g0017071 RchiOBHm_Chr5g0017091 RchiOBHm_Chr5g0017121 RchiOBHm_Chr5g0017141 RchiOBHm_Chr5g0017291 RchiOBHm_Chr5g0017321 RchiOBHm_Chr5g0017401 RchiOBHm_Chr5g0019001 RchiOBHm_Chr5g0019071 RchiOBHm_Chr5g0028941 RchiOBHm_Chr5g0052141 RchiOBHm_Chr5g0054721 RchiOBHm_Chr5g0054801 RchiOBHm_Chr5g0054931 RchiOBHm_Chr5g0054941 RchiOBHm_Chr6g0259291 RchiOBHm_Chr6g0292991 RchiOBHm_Chr6g0306911
rosa_laevigata RLG00000009465 RLG00000009468 RLG00000010755 RLG00000011976 RLG00000014541 RLG00000014542 RLG00000015169 RLG00000022308 RLG00000027529 RLG00000029324 RLG00000030244 RLG00000030245 RLG00000032210 RLG00000032211 RLG00000032257 RLG00000032259 RLG00000032260 RLG00000032261 RLG00000032262 RLG00000032268 RLG00000032269 RLG00000032272 RLG00000032282 RLG00000032283 RLG00000032308 RLG00000032413 RLG00000033129 RLG00000034945
rosa_multiflora Rmu_co8241929.1_g000001 Rmu_co8269227.1_g000001 Rmu_co8461259.1_g000001 Rmu_co8495385.1_g000001 Rmu_sc0000028.1_g000003 Rmu_sc0000083.1_g000009 Rmu_sc0000083.1_g000010 Rmu_sc0000083.1_g000031 Rmu_sc0000083.1_g000043 Rmu_sc0000083.1_g000046 Rmu_sc0000083.1_g000047 Rmu_sc0000563.1_g000024 Rmu_sc0000842.1_g000012 Rmu_sc0001235.1_g000001 Rmu_sc0001235.1_g000005 Rmu_sc0001235.1_g000009 Rmu_sc0001235.1_g000015 Rmu_sc0001235.1_g000016 Rmu_sc0001235.1_g000017 Rmu_sc0001473.1_g000036 Rmu_sc0001607.1_g000016 Rmu_sc0001788.1_g000006 Rmu_sc0002018.1_g000008 Rmu_sc0002028.1_g000001 Rmu_sc0002680.1_g000011 Rmu_sc0002798.1_g000023 Rmu_sc0002886.1_g000023 Rmu_sc0002989.1_g000012 Rmu_sc0003064.1_g000009 Rmu_sc0003382.1_g000006 Rmu_sc0003630.1_g000002 Rmu_sc0003630.1_g000047 Rmu_sc0003630.1_g000066 Rmu_sc0003630.1_g000067 Rmu_sc0003649.1_g000010 Rmu_sc0003688.1_g000005 Rmu_sc0003920.1_g000007 Rmu_sc0004765.1_g000007 Rmu_sc0004765.1_g000008 Rmu_sc0004920.1_g000011 Rmu_sc0005223.1_g000008 Rmu_sc0005223.1_g000010 Rmu_sc0005223.1_g000015 Rmu_sc0005223.1_g000016 Rmu_sc0005319.1_g000014 Rmu_sc0005319.1_g000015 Rmu_sc0005319.1_g000018 Rmu_sc0005319.1_g000019 Rmu_sc0005319.1_g000025 Rmu_sc0005418.1_g000007 Rmu_sc0005418.1_g000012 Rmu_sc0006106.1_g000011 Rmu_sc0006914.1_g000012 Rmu_sc0007023.1_g000001 Rmu_sc0007662.1_g000001 Rmu_sc0007920.1_g000002 Rmu_sc0007920.1_g000013 Rmu_sc0008053.1_g000016 Rmu_sc0008053.1_g000017 Rmu_sc0008053.1_g000018 Rmu_sc0008053.1_g000023 Rmu_sc0008053.1_g000050 Rmu_sc0009042.1_g000007 Rmu_sc0009042.1_g000008 Rmu_sc0010642.1_g000008 Rmu_sc0011109.1_g000002 Rmu_sc0011314.1_g000006 Rmu_sc0011974.1_g000006 Rmu_sc0012179.1_g000009 Rmu_sc0015169.1_g000001 Rmu_sc0016368.1_g000002 Rmu_sc0022659.1_g000001 Rmu_sc0027371.1_g000001 Rmu_sc0028213.1_g000001 Rmu_sc0034526.1_g000001 Rmu_sc0039366.1_g000002 Rmu_ssc0000050.1_g000084 Rmu_ssc0000050.1_g000088 Rmu_ssc0000167.1_g000017
rosa_roxburghii Rroxscaffold_1G00011340 Rroxscaffold_1G00025710 Rroxscaffold_1G00025870 Rroxscaffold_1G00026070 Rroxscaffold_1G00026170 Rroxscaffold_1G00026200 Rroxscaffold_1G00026270 Rroxscaffold_1G00050950 Rroxscaffold_1G00058900 Rroxscaffold_1G00060040 Rroxscaffold_1G00060060 Rroxscaffold_1G00060070 Rroxscaffold_1G00060080 Rroxscaffold_1G00060110 Rroxscaffold_1G00060120 Rroxscaffold_1G00060140 Rroxscaffold_1G00060170 Rroxscaffold_1G00060210 Rroxscaffold_1G00060220 Rroxscaffold_1G00060240 Rroxscaffold_1G00060250 Rroxscaffold_1G00060260 Rroxscaffold_1G00060300 Rroxscaffold_1G00060320 Rroxscaffold_1G00060330 Rroxscaffold_1G00060900 Rroxscaffold_1G00060920 Rroxscaffold_1G00073980 Rroxscaffold_2G00077560 Rroxscaffold_2G00077580 Rroxscaffold_2G00146990 Rroxscaffold_3G00236200 Rroxscaffold_3G00236210 Rroxscaffold_3G00236220 Rroxscaffold_4G00292760 Rroxscaffold_4G00315200 Rroxscaffold_4G00315240 Rroxscaffold_4G00326240 Rroxscaffold_4G00328980 Rroxscaffold_5G00341890 Rroxscaffold_7G00161350 Rroxscaffold_7G00174490 Rroxscaffold_7G00206550
rosa_rugosa Rorug01G0093000 Rorug01G0093100 Rorug01G0128800 Rorug01G0312000 Rorug01G0312000 Rorug02G0581100 Rorug03G0224600 Rorug05G0028500 Rorug05G0032700 Rorug05G0032800 Rorug05G0032900 Rorug05G0032900 Rorug05G0033000 Rorug05G0033100 Rorug05G0033300 Rorug05G0033400 Rorug05G0033900 Rorug05G0034900 Rorug05G0035000 Rorug05G0046900 Rorug05G0047500 Rorug05G0047500 Rorug05G0111600 Rorug05G0287900 Rorug05G0288700 Rorug05G0586900 Rorug06G0231700 Rorug06G0354300 Rorug06G0354400
rosa_samantha Rh1DG122200 Rh2DG686800 Rh5BG136600 Rh6AG344500 Rh6BG351900 Rh6CG359000 Rh6CG359100 Rh6CG387600 Rh6CG480500 Rh6DG344600 Rh6DG467400
rosa_wichuraiana Rw0G010250 Rw0G014820 Rw0G021400 Rw0G023100 Rw1G009450 Rw1G012100 Rw1G012180 Rw1G028360 Rw2G054260 Rw3G024370 Rw3G024420 Rw3G024470 Rw5G010850 Rw5G011010 Rw5G011020 Rw5G011050 Rw5G011080 Rw5G011120 Rw5G012300 Rw5G018470 Rw5G033830 Rw6G008930 Rw6G030070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 349
AccI GTMKAC 4 cut(s) 812, 1287, 1297, 1461
AciI CCGC 5 cut(s) 47, 111, 169, 629, 982
AclWI GGATC 2 cut(s) 1007, 2122
AcoI YGGCCR 1 cut(s) 1962
AcsI RAATTY 5 cut(s) 443, 931, 996, 1071, 1203
AcuI CTGAAG 2 cut(s) 1572, 1677
AfaI GTAC 6 cut(s) 55, 359, 410, 556, 1130, 2173
AfiI CCNNNNNNNGG 2 cut(s) 43, 806
AflIII ACRYGT 2 cut(s) 405, 2202
AgeI ACCGGT 1 cut(s) 221
AgsI TTSAA 6 cut(s) 646, 668, 1120, 1135, 1529, 1764
AjnI CCWGG 6 cut(s) 91, 159, 838, 937, 1740, 2167
Alw21I GWGCWC 6 cut(s) 342, 929, 1497, 1567, 1725, 2106
Alw26I GTCTC 1 cut(s) 2090
AlwI GGATC 2 cut(s) 1007, 2122
AlwNI CAGNNNCTG 1 cut(s) 77
Ama87I CYCGRG 1 cut(s) 1724
AoxI GGCC 6 cut(s) 637, 711, 797, 836, 1962, 2006
ApeKI GCWGC 7 cut(s) 71, 96, 612, 1547, 1556, 1622, 2141
ApoI RAATTY 5 cut(s) 443, 931, 996, 1071, 1203
AsiGI ACCGGT 1 cut(s) 221
Asp700I GAANNNNTTC 2 cut(s) 690, 761
AspS9I GGNCC 4 cut(s) 166, 711, 1744, 2165
AsuHPI GGTGA 4 cut(s) 927, 1709, 1963, 2109
AvaI CYCGRG 1 cut(s) 1724
AvaII GGWCC 3 cut(s) 166, 1744, 2165
BaeGI GKGCMC 1 cut(s) 1452
BalI TGGCCA 1 cut(s) 1964
BanI GGYRCC 1 cut(s) 349
BanII GRGCYC 1 cut(s) 342
BarI GAAGNNNNNNTAC 2 cut(s) 1437, 1469
BbsI GAAGAC 1 cut(s) 2205
Bbv12I GWGCWC 6 cut(s) 342, 929, 1497, 1567, 1725, 2106
BbvI GCAGC 7 cut(s) 83, 83, 624, 1559, 1568, 1634, 2128
BccI CCATC 5 cut(s) 863, 870, 1942, 2147, 2219
BceAI ACGGC 2 cut(s) 377, 698
BciT130I CCWGG 6 cut(s) 93, 161, 840, 939, 1742, 2169
BciVI GTATCC 1 cut(s) 1730
BcoDI GTCTC 1 cut(s) 2090
BfaI CTAG 5 cut(s) 51, 1568, 1845, 2036, 2187
BfmI CTRYAG 5 cut(s) 472, 610, 813, 1716, 2073
BfuI GTATCC 1 cut(s) 1730
BisI GCNGC 9 cut(s) 48, 72, 97, 112, 613, 1548, 1557, 1623, 2142
BlpI GCTNAGC 1 cut(s) 1861
BlsI GCNGC 9 cut(s) 49, 73, 98, 113, 614, 1549, 1558, 1624, 2143
BmcAI AGTACT 2 cut(s) 55, 1130
Bme1390I CCNGG 6 cut(s) 93, 161, 840, 939, 1742, 2169
Bme18I GGWCC 3 cut(s) 166, 1744, 2165
BmeT110I CYCGRG 1 cut(s) 1724
BmgT120I GGNCC 4 cut(s) 166, 711, 1744, 2165
BmiI GGNNCC 4 cut(s) 351, 900, 1186, 1746
BmrFI CCNGG 6 cut(s) 93, 161, 840, 939, 1742, 2169
BmrI ACTGGG 2 cut(s) 462, 531
BmsI GCATC 3 cut(s) 1207, 1847, 1878
BmuI ACTGGG 2 cut(s) 462, 531
BpiI GAAGAC 1 cut(s) 2205
Bpu1102I GCTNAGC 1 cut(s) 1861
BpuEI CTTGAG 1 cut(s) 66
BsaAI YACGTR 1 cut(s) 408
BsaJI CCNNGG 8 cut(s) 159, 430, 478, 1188, 1477, 1884, 2028, 2130
BsaWI WCCGGW 1 cut(s) 221
BsaXI ACNNNNNCTCC 4 cut(s) 58, 88, 1173, 1203
Bsc4I CCNNNNNNNGG 2 cut(s) 43, 806
Bse118I RCCGGY 2 cut(s) 221, 1346
Bse1I ACTGG 6 cut(s) 457, 537, 640, 829, 1173, 2178
Bse3DI GCAATG 2 cut(s) 316, 624
BseBI CCWGG 6 cut(s) 93, 161, 840, 939, 1742, 2169
BseDI CCNNGG 8 cut(s) 159, 430, 478, 1188, 1477, 1884, 2028, 2130
BseGI GGATG 2 cut(s) 531, 862
BseLI CCNNNNNNNGG 2 cut(s) 43, 806
BseMI GCAATG 2 cut(s) 316, 624
BseMII CTCAG 1 cut(s) 2091
BseNI ACTGG 6 cut(s) 457, 537, 640, 829, 1173, 2178
BseRI GAGGAG 1 cut(s) 1573
BseSI GKGCMC 1 cut(s) 1452
BseXI GCAGC 7 cut(s) 83, 83, 624, 1559, 1568, 1634, 2128
BsgI GTGCAG 2 cut(s) 530, 1233
Bsh1285I CGRYCG 1 cut(s) 982
BshFI GGCC 6 cut(s) 639, 713, 799, 838, 1964, 2008
BshNI GGYRCC 1 cut(s) 349
BshTI ACCGGT 1 cut(s) 221
BsiEI CGRYCG 1 cut(s) 982
BsiHKAI GWGCWC 6 cut(s) 342, 929, 1497, 1567, 1725, 2106
BsiHKCI CYCGRG 1 cut(s) 1724
BsiSI CCGG 2 cut(s) 222, 1347
BsiWI CGTACG 1 cut(s) 408
BslFI GGGAC 2 cut(s) 611, 1741
BslI CCNNNNNNNGG 2 cut(s) 43, 806
BsmAI GTCTC 1 cut(s) 2090
BsmFI GGGAC 2 cut(s) 611, 1741
BsmI GAATGC 2 cut(s) 583, 1759
BsnI GGCC 6 cut(s) 639, 713, 799, 838, 1964, 2008
BsoBI CYCGRG 1 cut(s) 1724
Bsp1286I GDGCHC 7 cut(s) 342, 929, 1452, 1497, 1567, 1725, 2106
Bsp143I GATC 5 cut(s) 298, 1012, 1511, 2077, 2114
Bsp1720I GCTNAGC 1 cut(s) 1861
Bsp19I CCATGG 3 cut(s) 1477, 1884, 2028
BspACI CCGC 5 cut(s) 47, 111, 169, 629, 982
BspANI GGCC 6 cut(s) 639, 713, 799, 838, 1964, 2008
BspCNI CTCAG 1 cut(s) 2092
BspHI TCATGA 1 cut(s) 1489
BspLI GGNNCC 4 cut(s) 351, 900, 1186, 1746
BspMAI CTGCAG 2 cut(s) 614, 2077
BspPI GGATC 2 cut(s) 1007, 2122
BspT107I GGYRCC 1 cut(s) 349
BsrDI GCAATG 2 cut(s) 316, 624
BsrFI RCCGGY 2 cut(s) 221, 1346
BsrI ACTGG 6 cut(s) 457, 537, 640, 829, 1173, 2178
BssAI RCCGGY 2 cut(s) 221, 1346
BssECI CCNNGG 8 cut(s) 159, 430, 478, 1188, 1477, 1884, 2028, 2130
BssMI GATC 5 cut(s) 298, 1012, 1511, 2077, 2114
BssNAI GTATAC 3 cut(s) 1288, 1298, 1462
BssT1I CCWWGG 5 cut(s) 478, 1477, 1884, 2028, 2130
Bst1107I GTATAC 3 cut(s) 1288, 1298, 1462
Bst2UI CCWGG 6 cut(s) 93, 161, 840, 939, 1742, 2169
Bst4CI ACNGT 4 cut(s) 357, 559, 1107, 1717
Bst6I CTCTTC 2 cut(s) 1491, 2088
BstAPI GCANNNNNTGC 1 cut(s) 77
BstBAI YACGTR 1 cut(s) 408
BstC8I GCNNGC 6 cut(s) 769, 836, 914, 1348, 1867, 2192
BstDEI CTNAG 2 cut(s) 1861, 2100
BstDSI CCRYGG 4 cut(s) 1188, 1477, 1884, 2028
BstEII GGTNACC 1 cut(s) 818
BstF5I GGATG 2 cut(s) 531, 862
BstKTI GATC 5 cut(s) 301, 1015, 1514, 2080, 2117
BstMAI GTCTC 1 cut(s) 2090
BstMBI GATC 5 cut(s) 298, 1012, 1511, 2077, 2114
BstMCI CGRYCG 1 cut(s) 982
BstMWI GCNNNNNNNGC 9 cut(s) 77, 626, 773, 1272, 1553, 1556, 1562, 1866, 1970
BstNI CCWGG 6 cut(s) 93, 161, 840, 939, 1742, 2169
BstNSI RCATGY 2 cut(s) 2194, 2206
BstPI GGTNACC 1 cut(s) 818
BstSCI CCNGG 6 cut(s) 91, 159, 838, 937, 1740, 2167
BstSFI CTRYAG 5 cut(s) 472, 610, 813, 1716, 2073
BstSLI GKGCMC 1 cut(s) 1452
BstV1I GCAGC 7 cut(s) 83, 83, 624, 1559, 1568, 1634, 2128
BstV2I GAAGAC 1 cut(s) 2205
BstXI CCANNNNNNTGG 2 cut(s) 342, 869
BstZ17I GTATAC 3 cut(s) 1288, 1298, 1462
BsuI GTATCC 1 cut(s) 1730
BsuRI GGCC 6 cut(s) 639, 713, 799, 838, 1964, 2008
BtgI CCRYGG 4 cut(s) 1188, 1477, 1884, 2028
BtsCI GGATG 2 cut(s) 531, 862
BtsI GCAGTG 1 cut(s) 490
BtsIMutI CAGTG 5 cut(s) 490, 544, 681, 836, 2185
Cac8I GCNNGC 6 cut(s) 769, 836, 914, 1348, 1867, 2192
CaiI CAGNNNCTG 1 cut(s) 77
CciI TCATGA 1 cut(s) 1489
Cfr10I RCCGGY 2 cut(s) 221, 1346
Cfr13I GGNCC 4 cut(s) 166, 711, 1744, 2165
CseI GACGC 1 cut(s) 778
Csp6I GTAC 6 cut(s) 54, 358, 409, 555, 1129, 2172
CspAI ACCGGT 1 cut(s) 221
CspCI CAANNNNNGTGG 2 cut(s) 1815, 1850
CviQI GTAC 6 cut(s) 54, 358, 409, 555, 1129, 2172
DdeI CTNAG 2 cut(s) 1861, 2100
DpnI GATC 5 cut(s) 300, 1014, 1513, 2079, 2116
DpnII GATC 5 cut(s) 298, 1012, 1511, 2077, 2114
EaeI YGGCCR 1 cut(s) 1962
Eam1104I CTCTTC 2 cut(s) 1491, 2088
EarI CTCTTC 2 cut(s) 1491, 2088
Ecl136II GAGCTC 1 cut(s) 340
Eco130I CCWWGG 5 cut(s) 478, 1477, 1884, 2028, 2130
Eco147I AGGCCT 2 cut(s) 799, 2008
Eco24I GRGCYC 1 cut(s) 342
Eco32I GATATC 1 cut(s) 373
Eco47I GGWCC 3 cut(s) 166, 1744, 2165
Eco53kI GAGCTC 1 cut(s) 340
Eco57I CTGAAG 2 cut(s) 1572, 1677
Eco88I CYCGRG 1 cut(s) 1724
Eco91I GGTNACC 1 cut(s) 818
EcoICRI GAGCTC 1 cut(s) 340
EcoO65I GGTNACC 1 cut(s) 818
EcoRI GAATTC 1 cut(s) 443
EcoRII CCWGG 6 cut(s) 91, 159, 838, 937, 1740, 2167
EcoRV GATATC 1 cut(s) 373
EcoT14I CCWWGG 5 cut(s) 478, 1477, 1884, 2028, 2130
EcoT22I ATGCAT 1 cut(s) 423
EcoT38I GRGCYC 1 cut(s) 342
ErhI CCWWGG 5 cut(s) 478, 1477, 1884, 2028, 2130
FaqI GGGAC 2 cut(s) 611, 1741
FauNDI CATATG 1 cut(s) 2218
FblI GTMKAC 4 cut(s) 812, 1287, 1297, 1461
Fnu4HI GCNGC 9 cut(s) 48, 72, 97, 112, 613, 1548, 1557, 1623, 2142
FokI GGATG 2 cut(s) 518, 849
FriOI GRGCYC 1 cut(s) 342
Fsp4HI GCNGC 9 cut(s) 48, 72, 97, 112, 613, 1548, 1557, 1623, 2142
FspBI CTAG 5 cut(s) 51, 1568, 1845, 2036, 2187
GluI GCNGC 9 cut(s) 48, 72, 97, 112, 613, 1548, 1557, 1623, 2142
HaeIII GGCC 6 cut(s) 639, 713, 799, 838, 1964, 2008
HapII CCGG 2 cut(s) 222, 1347
HgaI GACGC 1 cut(s) 778
HincII GTYRAC 2 cut(s) 793, 1372
HindII GTYRAC 2 cut(s) 793, 1372
HindIII AAGCTT 1 cut(s) 1418
HinfI GANTC 3 cut(s) 12, 215, 1707
HpaII CCGG 2 cut(s) 222, 1347
HphI GGTGA 4 cut(s) 927, 1709, 1963, 2109
Hpy188I TCNGA 6 cut(s) 103, 370, 431, 1356, 1666, 1736
Hpy188III TCNNGA 7 cut(s) 302, 866, 1490, 1610, 1680, 1726, 1845
HpyAV CCTTC 5 cut(s) 631, 751, 842, 1000, 2033
HpyCH4III ACNGT 4 cut(s) 357, 559, 1107, 1717
HpyCH4IV ACGT 3 cut(s) 252, 407, 1788
HpyF10VI GCNNNNNNNGC 9 cut(s) 77, 626, 773, 1272, 1553, 1556, 1562, 1866, 1970
HpyF3I CTNAG 2 cut(s) 1861, 2100
HpySE526I ACGT 3 cut(s) 252, 407, 1788
KroI GCCGGC 1 cut(s) 1346
KroNI GCCGGC 1 cut(s) 1348
Kzo9I GATC 5 cut(s) 298, 1012, 1511, 2077, 2114
LmnI GCTCC 5 cut(s) 79, 904, 1562, 1673, 2032
Lsp1109I GCAGC 7 cut(s) 83, 83, 624, 1559, 1568, 1634, 2128
LweI GCATC 3 cut(s) 1207, 1847, 1878
MaeI CTAG 5 cut(s) 51, 1568, 1845, 2036, 2187
MaeII ACGT 3 cut(s) 252, 407, 1788
MalI GATC 5 cut(s) 300, 1014, 1513, 2079, 2116
MboI GATC 5 cut(s) 298, 1012, 1511, 2077, 2114
MhlI GDGCHC 7 cut(s) 342, 929, 1452, 1497, 1567, 1725, 2106
MlsI TGGCCA 1 cut(s) 1964
MluNI TGGCCA 1 cut(s) 1964
MlyI GAGTC 1 cut(s) 6
MmeI TCCRAC 1 cut(s) 1048
Mox20I TGGCCA 1 cut(s) 1964
Mph1103I ATGCAT 1 cut(s) 423
MroNI GCCGGC 1 cut(s) 1346
MroXI GAANNNNTTC 2 cut(s) 690, 761
MscI TGGCCA 1 cut(s) 1964
MseI TTAA 4 cut(s) 603, 705, 1823, 1901
MslI CAYNNNNRTG 6 cut(s) 588, 867, 1081, 1407, 1611, 2207
Msp20I TGGCCA 1 cut(s) 1964
MspA1I CMGCKG 1 cut(s) 1550
MspI CCGG 2 cut(s) 222, 1347
MspR9I CCNGG 6 cut(s) 93, 161, 840, 939, 1742, 2169
Mva1269I GAATGC 2 cut(s) 583, 1759
MvaI CCWGG 6 cut(s) 93, 161, 840, 939, 1742, 2169
MwoI GCNNNNNNNGC 9 cut(s) 77, 626, 773, 1272, 1553, 1556, 1562, 1866, 1970
NaeI GCCGGC 1 cut(s) 1348
NcoI CCATGG 3 cut(s) 1477, 1884, 2028
NdeI CATATG 1 cut(s) 2218
NdeII GATC 5 cut(s) 298, 1012, 1511, 2077, 2114
NgoMIV GCCGGC 1 cut(s) 1346
NlaIV GGNNCC 4 cut(s) 351, 900, 1186, 1746
NmuCI GTSAC 5 cut(s) 401, 559, 959, 1175, 1784
NsiI ATGCAT 1 cut(s) 423
NspI RCATGY 2 cut(s) 2194, 2206
PaeI GCATGC 1 cut(s) 2194
PagI TCATGA 1 cut(s) 1489
PceI AGGCCT 2 cut(s) 799, 2008
PciI ACATGT 1 cut(s) 2202
PcsI WCGNNNNNNNCGW 1 cut(s) 1185
PctI GAATGC 2 cut(s) 583, 1759
PdiI GCCGGC 1 cut(s) 1348
PdmI GAANNNNTTC 2 cut(s) 690, 761
PfeI GAWTC 2 cut(s) 215, 1707
Pfl23II CGTACG 1 cut(s) 408
PinAI ACCGGT 1 cut(s) 221
PkrI GCNGC 9 cut(s) 49, 73, 98, 113, 614, 1549, 1558, 1624, 2143
PleI GAGTC 1 cut(s) 6
PpsI GAGTC 1 cut(s) 6
Ppu21I YACGTR 1 cut(s) 408
PscI ACATGT 1 cut(s) 2202
Psp124BI GAGCTC 1 cut(s) 342
Psp6I CCWGG 6 cut(s) 91, 159, 838, 937, 1740, 2167
PspEI GGTNACC 1 cut(s) 818
PspGI CCWGG 6 cut(s) 91, 159, 838, 937, 1740, 2167
PspLI CGTACG 1 cut(s) 408
PspN4I GGNNCC 4 cut(s) 351, 900, 1186, 1746
PspPI GGNCC 4 cut(s) 166, 711, 1744, 2165
PstI CTGCAG 2 cut(s) 614, 2077
PstNI CAGNNNCTG 1 cut(s) 77
PvuII CAGCTG 1 cut(s) 1550
RsaI GTAC 6 cut(s) 55, 359, 410, 556, 1130, 2173
RsaNI GTAC 6 cut(s) 54, 358, 409, 555, 1129, 2172
RseI CAYNNNNRTG 6 cut(s) 588, 867, 1081, 1407, 1611, 2207
SacI GAGCTC 1 cut(s) 342
SaqAI TTAA 4 cut(s) 603, 705, 1823, 1901
SatI GCNGC 9 cut(s) 48, 72, 97, 112, 613, 1548, 1557, 1623, 2142
Sau3AI GATC 5 cut(s) 298, 1012, 1511, 2077, 2114
Sau96I GGNCC 4 cut(s) 166, 711, 1744, 2165
ScaI AGTACT 2 cut(s) 55, 1130
SchI GAGTC 1 cut(s) 6
ScrFI CCNGG 6 cut(s) 93, 161, 840, 939, 1742, 2169
SduI GDGCHC 7 cut(s) 342, 929, 1452, 1497, 1567, 1725, 2106
SfaNI GCATC 3 cut(s) 1207, 1847, 1878
SfcI CTRYAG 5 cut(s) 472, 610, 813, 1716, 2073
SinI GGWCC 3 cut(s) 166, 1744, 2165
SmiMI CAYNNNNRTG 6 cut(s) 588, 867, 1081, 1407, 1611, 2207
SmlI CTYRAG 1 cut(s) 81
SmoI CTYRAG 1 cut(s) 81
SphI GCATGC 1 cut(s) 2194
SseBI AGGCCT 2 cut(s) 799, 2008
SsiI CCGC 5 cut(s) 47, 111, 169, 629, 982
SspI AATATT 2 cut(s) 1630, 1942
SspMI CTAG 5 cut(s) 51, 1568, 1845, 2036, 2187
SstI GAGCTC 1 cut(s) 342
StuI AGGCCT 2 cut(s) 799, 2008
StyD4I CCNGG 6 cut(s) 91, 159, 838, 937, 1740, 2167
StyI CCWWGG 5 cut(s) 478, 1477, 1884, 2028, 2130
TaaI ACNGT 4 cut(s) 357, 559, 1107, 1717
TaiI ACGT 3 cut(s) 255, 410, 1791
TaqI TCGA 2 cut(s) 180, 1609
TatI WGTACW 2 cut(s) 53, 1128
TauI GCSGC 2 cut(s) 50, 114
TfiI GAWTC 2 cut(s) 215, 1707
Tru1I TTAA 4 cut(s) 603, 705, 1823, 1901
Tru9I TTAA 4 cut(s) 603, 705, 1823, 1901
TscAI CASTG 5 cut(s) 490, 544, 688, 836, 2185
TseFI GTSAC 5 cut(s) 401, 559, 959, 1175, 1784
TseI GCWGC 7 cut(s) 71, 96, 612, 1547, 1556, 1622, 2141
Tsp45I GTSAC 5 cut(s) 401, 559, 959, 1175, 1784
TspGWI ACGGA 2 cut(s) 1177, 1194
TspRI CASTG 5 cut(s) 490, 544, 688, 836, 2185
VpaK11BI GGWCC 3 cut(s) 166, 1744, 2165
XapI RAATTY 5 cut(s) 443, 931, 996, 1071, 1203
XbaI TCTAGA 1 cut(s) 1844
XceI RCATGY 2 cut(s) 2194, 2206
XcmI CCANNNNNNNNNTGG 1 cut(s) 836
XmiI GTMKAC 4 cut(s) 812, 1287, 1297, 1461
XmnI GAANNNNTTC 2 cut(s) 690, 761
XspI CTAG 5 cut(s) 51, 1568, 1845, 2036, 2187
ZrmI AGTACT 2 cut(s) 55, 1130
Zsp2I ATGCAT 1 cut(s) 423
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.