Prupe.4G093300_v2.0.a1

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0000066
Physical Location & Seq
Reverse (-)
4701040 .. 4704322
3283 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G093300.1

Sequence Viewer

Length: 2259 bp
ATGCCATTCTTACATGAGAGGATGCTTATTATGCAAGTCTCGTTGGTAATGGCAGCGGTGGCGGCTACAGCAGCTCAATTAGCTCTCCCAAACTGCCTGGACAAGTGCGGAAATGTCACAATTCCATACCCATTTGGCGTTACTAAAAAGTGTTACATGGCACCTGAGTTTTTCATCACATGCGCGGCTAACGGAATAGCATTCTTGGGGACAACCAACATTCCTGTTACCAACATATCCGTGGATCTCGGTGAGATCCAAGTACAGCAGTATAGGGCCTATGACTGCTACGACGAGCTGGGCAACAATAGCTACAACACCCCGGAGTTCTGGGTGGCTCCTCCTTACACCATCTCCGGTACCAAAAACAAGTTCATGGCCGTTGGTTGCGATACTTATGCAACTTTTAGAGGCTACCGTGCAAACCAAGAAAGATTCATCACCGGTTGCATGTCCTTGTGTGACAGCCTCAACAGTGTTGAGCAAGACTCTTGTTCTGGCATCGGGTGTTGCCAAACTTCCATCCCCGACGGTCTTAAAAATCGTACTGTGACGTTGAGAAGCTATTACAATCATACGTATATTAGGAGCTTTAACCCCTGCAGCTACGCCTTTGTTGTGCAAGAAGGCCACTTCAGATTCTCCAAGACAAGTTTTCAACAAGTCAACCACACAGAGCAGCTTCCAATGATCCTTAACTGGGAAATTGGAAATGAGCCATGCGATGCAGCTCAAGAGGCGACTCAGAATTCTTTATGCAAGGCACATAGCAAGTGTGTCAAACCCAATAATCGCTCTACAGGTTACATTTGCCAGTGCTTGCCAGGTTATGAAGGGAACCCTTACCTCCCCAATGGTTGCCAAGATATTGATGAGTGCAAGGCTTCAAACTCCTGCAGTATTGGAGTGTGCGTAAATTCCCCTGGAGATTATTCTTGTCAATGTCCCAAAGGGTATAAAAACGACGGCATGAATCCGAAGAGTTGCAGCAAAGACAATCGATCAAAGACCATTCTCCTGCTTATCATTTCATTAGGTGTCAGCACAGGGTTATTGATTTTGCTGGGGGCAATTTCATGGTTATATTGCGGTCTGCAAAGAAGAAAGTTCATCAAACTCAAAGAAAAATACTTTAAAGAAAATGGTGGTTTATTGTTAGAACAACACCTTGCTAGCCAAGGAGACTCTATGGAGACAACCAGGATCTTTACAGAAGAAGCACTTGAAAAGGCCACAAACAATTACCATGAAAGTAGAGTGCTTGGCGAAGGAGGCTATGGAACTGTTTACAAAGGAATATTAGCAGATAACAAAGTGGTTGCCATAAAGAAGTCCAAAATTGGTGCCTCAACCCAGAAGGAGCAGTTTGTTAATGAGATGATTGTTCTTTCTCAAATCAACCATAGAAATGTGGTGAGGCTATTGGGTTGCTGTTTAGAGACACCAGTTCCTTTACTAGTTTATGAGTTCGTCACCAATGGCACTCTTTTCGCCCACATTCATAATATAAGGGACAAAAGATCATCACTTCCATGGGACTTAAGAATGAAGATAGCAACAGAAACTTCGGGAGCACTAGCATACTTACACTCCTCCACTTCCATGCCAATCATACACCGGGATGTCAAAACAACGAACATACTGTTAGATGAAAATTACACAGCGAAAGTGTCAGACTTTGGAGGTTCAAAATTGATTCCCGTGGATCAAACTCAACTAACAACTTTAGTGCAGGGGACACTCGGATACTTAGACCCCGAATACTTCCTTTCAAATCAACTAACGGAAAAGAGTGATGTTTATAGTTTTGGAGTTGTCCTAGCAGAGCTACTAACAAGTAGAGTAGCACTTTGTTTTGACAGGCCTGAGGCAGAGAGAAACCTAGCAAACTTCTTTGTTTGTTCAGTGGAGGAAGATCGCTTGAATCAAATTCTTGACGATGACATAGTCAACGATGGAAATATTGAGACTCTGAAAAATGTGGCCATTCTCGCGAAAAGATGCTTGAGGTTACAAGGGGAGGAAAGGCCTACAATGAAAGAGGTAGCCTTGGAGCTAGAAGGCATGAGAATTATGGCAAAACATCCATGGGGGAAAGCTGATTGTCCAGAAGAGACTGAGCACTTGCTTGGTTCAGGCAAGTCAGATGCTTATCGTGTGGATGATACAGCAGCAGATTGTGGTCATCCTAGTGGTACAAGTAGCGGATATGACAGCATGCAAATCCAGATGTTAACGTCGTATGGTGATGGGCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK

Protein Analysis

753

Amino Acids

83.38

Weight (kDa)

5.84

Isoelectric Point (pI)

34.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21210 AT1G21230 AT1G21240 AT1G21240 AT1G21245 AT1G21250 AT1G21270 AT1G22720 AT3G25490
fragaria_vesca FvH4_2g35051 FvH4_2g35051 FvH4_2g35060 FvH4_3g10281 FvH4_3g10285 FvH4_3g10286 FvH4_3g10311 FvH4_3g10311 FvH4_3g10320 FvH4_3g10332 FvH4_3g10340 FvH4_3g10350 FvH4_3g10360 FvH4_3g10390 FvH4_3g10412 FvH4_3g10413 FvH4_3g10461 FvH4_3g10462 FvH4_3g10470 FvH4_3g10510 FvH4_3g10520 FvH4_3g10530 FvH4_3g10550 FvH4_3g10560 FvH4_3g10611 FvH4_3g11730 FvH4_6g53140 FvH4_6g53142 FvH4_7g31880 FvH4_7g31910 FvH4_7g31920
malus_domestica MD05G1269900.v1.1 MD05G1270000.v1.1 MD05G1270200.v1.1 MD05G1270400.v1.1 MD05G1270500.v1.1 MD05G1270600.v1.1 MD05G1270800.v1.1 MD05G1270900.v1.1 MD07G1183800.v1.1 MD08G1107600.v1.1 MD10G1248500.v1.1 MD10G1249200.v1.1 MD10G1249500.v1.1 MD10G1250000.v1.1 MD10G1250500.v1.1 MD10G1250900.v1.1 MD10G1251200.v1.1 MD10G1251400.v1.1 MD13G1004600.v1.1 MD15G1088400.v1.1
prunus_persica Prupe.1G442400_v2.0.a1 Prupe.1G442500_v2.0.a1 Prupe.2G223400_v2.0.a1 Prupe.4G091900_v2.0.a1 Prupe.4G093200_v2.0.a1 Prupe.4G093300_v2.0.a1 Prupe.4G093400_v2.0.a1 Prupe.4G093500_v2.0.a1 Prupe.4G094100_v2.0.a1 Prupe.4G094200_v2.0.a1 Prupe.4G094300_v2.0.a1 Prupe.6G358300_v2.0.a1
pyrus_communis pycom08g08990 pycom10g20660 pycom10g20670 pycom10g20680 pycom10g20710 pycom10g20740 pycom13g00400 pycom15g08290
rosa_chinensis RchiOBHm_Chr1g0326861 RchiOBHm_Chr1g0326871 RchiOBHm_Chr1g0331391 RchiOBHm_Chr1g0336911 RchiOBHm_Chr1g0364001 RchiOBHm_Chr2g0174541 RchiOBHm_Chr2g0175041 RchiOBHm_Chr2g0175101 RchiOBHm_Chr2g0175121 RchiOBHm_Chr3g0486331 RchiOBHm_Chr5g0015581 RchiOBHm_Chr5g0016551 RchiOBHm_Chr5g0016561 RchiOBHm_Chr5g0016581 RchiOBHm_Chr5g0016601 RchiOBHm_Chr5g0016611 RchiOBHm_Chr5g0016641 RchiOBHm_Chr5g0016661 RchiOBHm_Chr5g0016691 RchiOBHm_Chr5g0016701 RchiOBHm_Chr5g0016731 RchiOBHm_Chr5g0016741 RchiOBHm_Chr5g0016811 RchiOBHm_Chr5g0016841 RchiOBHm_Chr5g0016871 RchiOBHm_Chr5g0016881 RchiOBHm_Chr5g0016931 RchiOBHm_Chr5g0017021 RchiOBHm_Chr5g0017041 RchiOBHm_Chr5g0017071 RchiOBHm_Chr5g0017091 RchiOBHm_Chr5g0017121 RchiOBHm_Chr5g0017141 RchiOBHm_Chr5g0017291 RchiOBHm_Chr5g0017321 RchiOBHm_Chr5g0017401 RchiOBHm_Chr5g0019001 RchiOBHm_Chr5g0019071 RchiOBHm_Chr5g0028941 RchiOBHm_Chr5g0052141 RchiOBHm_Chr5g0054721 RchiOBHm_Chr5g0054801 RchiOBHm_Chr5g0054931 RchiOBHm_Chr5g0054941 RchiOBHm_Chr6g0259291 RchiOBHm_Chr6g0292991 RchiOBHm_Chr6g0306911
rosa_laevigata RLG00000009465 RLG00000009468 RLG00000010755 RLG00000011976 RLG00000014541 RLG00000014542 RLG00000015169 RLG00000022308 RLG00000027529 RLG00000029324 RLG00000030244 RLG00000030245 RLG00000032210 RLG00000032211 RLG00000032257 RLG00000032259 RLG00000032260 RLG00000032261 RLG00000032262 RLG00000032268 RLG00000032269 RLG00000032272 RLG00000032282 RLG00000032283 RLG00000032308 RLG00000032413 RLG00000033129 RLG00000034945
rosa_multiflora Rmu_co8241929.1_g000001 Rmu_co8269227.1_g000001 Rmu_co8461259.1_g000001 Rmu_co8495385.1_g000001 Rmu_sc0000028.1_g000003 Rmu_sc0000083.1_g000009 Rmu_sc0000083.1_g000010 Rmu_sc0000083.1_g000031 Rmu_sc0000083.1_g000043 Rmu_sc0000083.1_g000046 Rmu_sc0000083.1_g000047 Rmu_sc0000563.1_g000024 Rmu_sc0000842.1_g000012 Rmu_sc0001235.1_g000001 Rmu_sc0001235.1_g000005 Rmu_sc0001235.1_g000009 Rmu_sc0001235.1_g000015 Rmu_sc0001235.1_g000016 Rmu_sc0001235.1_g000017 Rmu_sc0001473.1_g000036 Rmu_sc0001607.1_g000016 Rmu_sc0001788.1_g000006 Rmu_sc0002018.1_g000008 Rmu_sc0002028.1_g000001 Rmu_sc0002680.1_g000011 Rmu_sc0002798.1_g000023 Rmu_sc0002886.1_g000023 Rmu_sc0002989.1_g000012 Rmu_sc0003064.1_g000009 Rmu_sc0003382.1_g000006 Rmu_sc0003630.1_g000002 Rmu_sc0003630.1_g000047 Rmu_sc0003630.1_g000066 Rmu_sc0003630.1_g000067 Rmu_sc0003649.1_g000010 Rmu_sc0003688.1_g000005 Rmu_sc0003920.1_g000007 Rmu_sc0004765.1_g000007 Rmu_sc0004765.1_g000008 Rmu_sc0004920.1_g000011 Rmu_sc0005223.1_g000008 Rmu_sc0005223.1_g000010 Rmu_sc0005223.1_g000015 Rmu_sc0005223.1_g000016 Rmu_sc0005319.1_g000014 Rmu_sc0005319.1_g000015 Rmu_sc0005319.1_g000018 Rmu_sc0005319.1_g000019 Rmu_sc0005319.1_g000025 Rmu_sc0005418.1_g000007 Rmu_sc0005418.1_g000012 Rmu_sc0006106.1_g000011 Rmu_sc0006914.1_g000012 Rmu_sc0007023.1_g000001 Rmu_sc0007662.1_g000001 Rmu_sc0007920.1_g000002 Rmu_sc0007920.1_g000013 Rmu_sc0008053.1_g000016 Rmu_sc0008053.1_g000017 Rmu_sc0008053.1_g000018 Rmu_sc0008053.1_g000023 Rmu_sc0008053.1_g000050 Rmu_sc0009042.1_g000007 Rmu_sc0009042.1_g000008 Rmu_sc0010642.1_g000008 Rmu_sc0011109.1_g000002 Rmu_sc0011314.1_g000006 Rmu_sc0011974.1_g000006 Rmu_sc0012179.1_g000009 Rmu_sc0015169.1_g000001 Rmu_sc0016368.1_g000002 Rmu_sc0022659.1_g000001 Rmu_sc0027371.1_g000001 Rmu_sc0028213.1_g000001 Rmu_sc0034526.1_g000001 Rmu_sc0039366.1_g000002 Rmu_ssc0000050.1_g000084 Rmu_ssc0000050.1_g000088 Rmu_ssc0000167.1_g000017
rosa_roxburghii Rroxscaffold_1G00011340 Rroxscaffold_1G00025710 Rroxscaffold_1G00025870 Rroxscaffold_1G00026070 Rroxscaffold_1G00026170 Rroxscaffold_1G00026200 Rroxscaffold_1G00026270 Rroxscaffold_1G00050950 Rroxscaffold_1G00058900 Rroxscaffold_1G00060040 Rroxscaffold_1G00060060 Rroxscaffold_1G00060070 Rroxscaffold_1G00060080 Rroxscaffold_1G00060110 Rroxscaffold_1G00060120 Rroxscaffold_1G00060140 Rroxscaffold_1G00060170 Rroxscaffold_1G00060210 Rroxscaffold_1G00060220 Rroxscaffold_1G00060240 Rroxscaffold_1G00060250 Rroxscaffold_1G00060260 Rroxscaffold_1G00060300 Rroxscaffold_1G00060320 Rroxscaffold_1G00060330 Rroxscaffold_1G00060900 Rroxscaffold_1G00060920 Rroxscaffold_1G00073980 Rroxscaffold_2G00077560 Rroxscaffold_2G00077580 Rroxscaffold_2G00146990 Rroxscaffold_3G00236200 Rroxscaffold_3G00236210 Rroxscaffold_3G00236220 Rroxscaffold_4G00292760 Rroxscaffold_4G00315200 Rroxscaffold_4G00315240 Rroxscaffold_4G00326240 Rroxscaffold_4G00328980 Rroxscaffold_5G00341890 Rroxscaffold_7G00161350 Rroxscaffold_7G00174490 Rroxscaffold_7G00206550
rosa_rugosa Rorug01G0093000 Rorug01G0093100 Rorug01G0128800 Rorug01G0312000 Rorug01G0312000 Rorug02G0581100 Rorug03G0224600 Rorug05G0028500 Rorug05G0032700 Rorug05G0032800 Rorug05G0032900 Rorug05G0032900 Rorug05G0033000 Rorug05G0033100 Rorug05G0033300 Rorug05G0033400 Rorug05G0033900 Rorug05G0034900 Rorug05G0035000 Rorug05G0046900 Rorug05G0047500 Rorug05G0047500 Rorug05G0111600 Rorug05G0287900 Rorug05G0288700 Rorug05G0586900 Rorug06G0231700 Rorug06G0354300 Rorug06G0354400
rosa_samantha Rh1DG122200 Rh2DG686800 Rh5BG136600 Rh6AG344500 Rh6BG351900 Rh6CG359000 Rh6CG359100 Rh6CG387600 Rh6CG480500 Rh6DG344600 Rh6DG467400
rosa_wichuraiana Rw0G010250 Rw0G014820 Rw0G021400 Rw0G023100 Rw1G009450 Rw1G012100 Rw1G012180 Rw1G028360 Rw2G054260 Rw3G024370 Rw3G024420 Rw3G024470 Rw5G010850 Rw5G011010 Rw5G011020 Rw5G011050 Rw5G011080 Rw5G011120 Rw5G012300 Rw5G018470 Rw5G033830 Rw6G008930 Rw6G030070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 359
AccB1I GGYRCC 3 cut(s) 160, 359, 1343
AccII CGCG 2 cut(s) 185, 1994
AciI CCGC 6 cut(s) 56, 62, 108, 185, 1089, 2205
AclWI GGATC 5 cut(s) 250, 252, 685, 1211, 1713
AcoI YGGCCR 2 cut(s) 378, 1983
AcsI RAATTY 3 cut(s) 748, 916, 1929
AcuI CTGAAG 1 cut(s) 619
AfaI GTAC 4 cut(s) 264, 361, 547, 2197
AfiI CCNNNNNNNGG 1 cut(s) 700
AflII CTTAAG 1 cut(s) 1540
AgeI ACCGGT 1 cut(s) 443
AgsI TTSAA 6 cut(s) 659, 888, 1226, 1689, 1773, 1924
AhlI ACTAGT 1 cut(s) 1456
AjnI CCWGG 4 cut(s) 96, 823, 922, 1199
AjuI GAANNNNNNNTTGG 2 cut(s) 356, 388
Alw21I GWGCWC 2 cut(s) 1576, 2124
Alw26I GTCTC 6 cut(s) 43, 1176, 1187, 1433, 1961, 2108
AlwI GGATC 5 cut(s) 250, 252, 685, 1211, 1713
AoxI GGCC 7 cut(s) 276, 378, 628, 1230, 1862, 1983, 2027
ApeKI GCWGC 7 cut(s) 53, 71, 603, 679, 728, 987, 2171
ApoI RAATTY 3 cut(s) 748, 916, 1929
AsiGI ACCGGT 1 cut(s) 443
Asp718I GGTACC 1 cut(s) 359
AspLEI GCGC 1 cut(s) 185
AspS9I GGNCC 1 cut(s) 276
AsuC2I CCSGG 2 cut(s) 323, 1619
AsuHPI GGTGA 5 cut(s) 263, 433, 1426, 1465, 2258
AsuNHI GCTAGC 1 cut(s) 1172
AxyI CCTNAGG 1 cut(s) 1866
BalI TGGCCA 1 cut(s) 1985
BanI GGYRCC 3 cut(s) 160, 359, 1343
Bbv12I GWGCWC 2 cut(s) 1576, 2124
BbvI GCAGC 7 cut(s) 65, 83, 615, 691, 740, 999, 2183
BccI CCATC 4 cut(s) 359, 530, 1949, 2243
BceAI ACGGC 2 cut(s) 365, 982
BcgI CGANNNNNNTGC 4 cut(s) 380, 414, 1471, 1505
BciT130I CCWGG 4 cut(s) 98, 825, 924, 1201
BciVI GTATCC 1 cut(s) 1739
BcnI CCSGG 2 cut(s) 323, 1619
BcoDI GTCTC 6 cut(s) 43, 1176, 1187, 1433, 1961, 2108
BcuI ACTAGT 1 cut(s) 1456
BfaI CTAG 7 cut(s) 1173, 1457, 1577, 1820, 1883, 2057, 2190
BfmI CTRYAG 4 cut(s) 66, 601, 798, 895
BfrI CTTAAG 1 cut(s) 1540
BfuI GTATCC 1 cut(s) 1739
BisI GCNGC 9 cut(s) 54, 63, 72, 186, 604, 680, 729, 988, 2172
BlsI GCNGC 9 cut(s) 55, 64, 73, 187, 605, 681, 730, 989, 2173
Bme1390I CCNGG 6 cut(s) 98, 323, 825, 924, 1201, 1619
BmgT120I GGNCC 1 cut(s) 276
BmiI GGNNCC 5 cut(s) 162, 339, 361, 839, 1345
BmrFI CCNGG 6 cut(s) 98, 323, 825, 924, 1201, 1619
BmrI ACTGGG 1 cut(s) 709
BmsI GCATC 5 cut(s) 12, 510, 715, 1991, 2137
BmtI GCTAGC 1 cut(s) 1176
BmuI ACTGGG 1 cut(s) 709
BplI GAGNNNNNCTC 2 cut(s) 473, 505
BpmI CTGGAG 1 cut(s) 945
BpuEI CTTGAG 2 cut(s) 717, 2026
BpuMI CCSGG 2 cut(s) 323, 1619
Bsa29I ATCGAT 1 cut(s) 1000
BsaAI YACGTR 1 cut(s) 579
BsaBI GATNNNNATC 1 cut(s) 2151
BsaJI CCNNGG 8 cut(s) 240, 321, 922, 1177, 1532, 1701, 2049, 2087
BsaWI WCCGGW 2 cut(s) 356, 443
BsaXI ACNNNNNCTCC 6 cut(s) 338, 368, 897, 927, 1574, 1604
Bsc4I CCNNNNNNNGG 1 cut(s) 700
Bse118I RCCGGY 1 cut(s) 443
Bse1I ACTGG 3 cut(s) 704, 814, 1445
Bse21I CCTNAGG 1 cut(s) 1866
Bse8I GATNNNNATC 1 cut(s) 2151
BseBI CCWGG 4 cut(s) 98, 825, 924, 1201
BseCI ATCGAT 1 cut(s) 1000
BseDI CCNNGG 8 cut(s) 240, 321, 922, 1177, 1532, 1701, 2049, 2087
BseGI GGATG 6 cut(s) 27, 522, 1627, 2083, 2167, 2185
BseJI GATNNNNATC 1 cut(s) 2151
BseLI CCNNNNNNNGG 1 cut(s) 700
BseMII CTCAG 4 cut(s) 156, 758, 1857, 2109
BseNI ACTGG 3 cut(s) 704, 814, 1445
BseRI GAGGAG 2 cut(s) 330, 1582
BseXI GCAGC 7 cut(s) 65, 83, 615, 691, 740, 999, 2183
BseYI CCCAGC 2 cut(s) 298, 1063
BsgI GTGCAG 1 cut(s) 1751
Bsh1236I CGCG 2 cut(s) 185, 1994
BshFI GGCC 7 cut(s) 278, 380, 630, 1232, 1864, 1985, 2029
BshNI GGYRCC 3 cut(s) 160, 359, 1343
BshTI ACCGGT 1 cut(s) 443
BshVI ATCGAT 1 cut(s) 1000
BsiHKAI GWGCWC 2 cut(s) 1576, 2124
BsiSI CCGG 4 cut(s) 323, 357, 444, 1618
BslFI GGGAC 5 cut(s) 223, 930, 1526, 1550, 1750
BslI CCNNNNNNNGG 1 cut(s) 700
BsmAI GTCTC 6 cut(s) 43, 1176, 1187, 1433, 1961, 2108
BsmFI GGGAC 5 cut(s) 223, 930, 1526, 1550, 1750
BsmI GAATGC 1 cut(s) 200
BsnI GGCC 7 cut(s) 278, 380, 630, 1232, 1864, 1985, 2029
Bsp1286I GDGCHC 2 cut(s) 1576, 2124
Bsp143I GATC 8 cut(s) 244, 255, 690, 1001, 1203, 1520, 1705, 1915
Bsp19I CCATGG 2 cut(s) 1532, 2087
Bsp68I TCGCGA 1 cut(s) 1994
BspACI CCGC 6 cut(s) 56, 62, 108, 185, 1089, 2205
BspANI GGCC 7 cut(s) 278, 380, 630, 1232, 1864, 1985, 2029
BspCNI CTCAG 4 cut(s) 157, 757, 1858, 2110
BspDI ATCGAT 1 cut(s) 1000
BspFNI CGCG 2 cut(s) 185, 1994
BspLI GGNNCC 5 cut(s) 162, 339, 361, 839, 1345
BspMAI CTGCAG 2 cut(s) 605, 899
BspOI GCTAGC 1 cut(s) 1176
BspPI GGATC 5 cut(s) 250, 252, 685, 1211, 1713
BspT107I GGYRCC 3 cut(s) 160, 359, 1343
BspTI CTTAAG 1 cut(s) 1540
BsrFI RCCGGY 1 cut(s) 443
BsrI ACTGG 3 cut(s) 704, 814, 1445
BssAI RCCGGY 1 cut(s) 443
BssECI CCNNGG 8 cut(s) 240, 321, 922, 1177, 1532, 1701, 2049, 2087
BssMI GATC 8 cut(s) 244, 255, 690, 1001, 1203, 1520, 1705, 1915
BssT1I CCWWGG 4 cut(s) 1177, 1532, 2049, 2087
Bst2UI CCWGG 4 cut(s) 98, 825, 924, 1201
Bst4CI ACNGT 6 cut(s) 419, 476, 533, 550, 1285, 1644
Bst6I CTCTTC 2 cut(s) 974, 2106
BstAFI CTTAAG 1 cut(s) 1540
BstBAI YACGTR 1 cut(s) 579
BstC8I GCNNGC 3 cut(s) 821, 1174, 2219
BstDEI CTNAG 5 cut(s) 165, 744, 1750, 1866, 2118
BstDSI CCRYGG 4 cut(s) 240, 1532, 1701, 2087
BstF5I GGATG 6 cut(s) 27, 522, 1627, 2083, 2167, 2185
BstFNI CGCG 2 cut(s) 185, 1994
BstHHI GCGC 1 cut(s) 185
BstKTI GATC 8 cut(s) 247, 258, 693, 1004, 1206, 1523, 1708, 1918
BstMAI GTCTC 6 cut(s) 43, 1176, 1187, 1433, 1961, 2108
BstMBI GATC 8 cut(s) 244, 255, 690, 1001, 1203, 1520, 1705, 1915
BstNI CCWGG 4 cut(s) 98, 825, 924, 1201
BstNSI RCATGY 3 cut(s) 183, 454, 2221
BstSCI CCNGG 6 cut(s) 96, 321, 823, 922, 1199, 1617
BstSFI CTRYAG 4 cut(s) 66, 601, 798, 895
BstSNI TACGTA 1 cut(s) 579
BstUI CGCG 2 cut(s) 185, 1994
BstV1I GCAGC 7 cut(s) 65, 83, 615, 691, 740, 999, 2183
BstX2I RGATCY 3 cut(s) 244, 255, 1203
BstYI RGATCY 3 cut(s) 244, 255, 1203
Bsu15I ATCGAT 1 cut(s) 1000
Bsu36I CCTNAGG 1 cut(s) 1866
BsuI GTATCC 1 cut(s) 1739
BsuRI GGCC 7 cut(s) 278, 380, 630, 1232, 1864, 1985, 2029
BsuTUI ATCGAT 1 cut(s) 1000
BtgI CCRYGG 4 cut(s) 240, 1532, 1701, 2087
BtgZI GCGATG 1 cut(s) 738
BtsCI GGATG 6 cut(s) 27, 522, 1627, 2083, 2167, 2185
BtsIMutI CAGTG 3 cut(s) 481, 821, 1911
BtuMI TCGCGA 1 cut(s) 1994
Cac8I GCNNGC 3 cut(s) 821, 1174, 2219
CfoI GCGC 1 cut(s) 185
Cfr10I RCCGGY 1 cut(s) 443
Cfr13I GGNCC 1 cut(s) 276
ClaI ATCGAT 1 cut(s) 1000
Csp6I GTAC 4 cut(s) 263, 360, 546, 2196
CspAI ACCGGT 1 cut(s) 443
CviQI GTAC 4 cut(s) 263, 360, 546, 2196
DdeI CTNAG 5 cut(s) 165, 744, 1750, 1866, 2118
DpnI GATC 8 cut(s) 246, 257, 692, 1003, 1205, 1522, 1707, 1917
DpnII GATC 8 cut(s) 244, 255, 690, 1001, 1203, 1520, 1705, 1915
DraI TTTAAA 1 cut(s) 1135
EaeI YGGCCR 2 cut(s) 378, 1983
Eam1104I CTCTTC 2 cut(s) 974, 2106
EarI CTCTTC 2 cut(s) 974, 2106
Eco105I TACGTA 1 cut(s) 579
Eco130I CCWWGG 4 cut(s) 1177, 1532, 2049, 2087
Eco147I AGGCCT 2 cut(s) 1864, 2029
Eco57I CTGAAG 1 cut(s) 619
Eco81I CCTNAGG 1 cut(s) 1866
EcoO109I RGGNCCY 1 cut(s) 276
EcoRI GAATTC 1 cut(s) 748
EcoRII CCWGG 4 cut(s) 96, 823, 922, 1199
EcoT14I CCWWGG 4 cut(s) 1177, 1532, 2049, 2087
ErhI CCWWGG 4 cut(s) 1177, 1532, 2049, 2087
FalI AAGNNNNNCTT 2 cut(s) 1206, 1238
FaqI GGGAC 5 cut(s) 223, 930, 1526, 1550, 1750
Fnu4HI GCNGC 9 cut(s) 54, 63, 72, 186, 604, 680, 729, 988, 2172
FokI GGATG 6 cut(s) 34, 509, 1634, 2070, 2172, 2174
Fsp4HI GCNGC 9 cut(s) 54, 63, 72, 186, 604, 680, 729, 988, 2172
FspBI CTAG 7 cut(s) 1173, 1457, 1577, 1820, 1883, 2057, 2190
GlaI GCGC 1 cut(s) 184
GluI GCNGC 9 cut(s) 54, 63, 72, 186, 604, 680, 729, 988, 2172
GsaI CCCAGC 2 cut(s) 302, 1067
GsuI CTGGAG 1 cut(s) 945
HaeIII GGCC 7 cut(s) 278, 380, 630, 1232, 1864, 1985, 2029
HapII CCGG 4 cut(s) 323, 357, 444, 1618
HhaI GCGC 1 cut(s) 185
Hin6I GCGC 1 cut(s) 183
HinP1I GCGC 1 cut(s) 183
HincII GTYRAC 3 cut(s) 667, 1951, 2235
HindII GTYRAC 3 cut(s) 667, 1951, 2235
HinfI GANTC 9 cut(s) 435, 488, 639, 742, 973, 1184, 1696, 1924, 1969
HpaI GTTAAC 1 cut(s) 2235
HpaII CCGG 4 cut(s) 323, 357, 444, 1618
HphI GGTGA 5 cut(s) 263, 433, 1426, 1465, 2258
Hpy166II GTNNAC 4 cut(s) 667, 1288, 1951, 2235
Hpy188I TCNGA 7 cut(s) 638, 747, 978, 1675, 1745, 1974, 2146
Hpy188III TCNNGA 6 cut(s) 734, 1569, 1934, 1993, 2108, 2227
Hpy8I GTNNAC 4 cut(s) 667, 1288, 1951, 2235
Hpy99I CGWCG 4 cut(s) 296, 533, 968, 2242
HpyAV CCTTC 5 cut(s) 620, 827, 1262, 1351, 2054
HpyCH4III ACNGT 6 cut(s) 419, 476, 533, 550, 1285, 1644
HpyCH4IV ACGT 3 cut(s) 554, 578, 2237
HpyF3I CTNAG 5 cut(s) 165, 744, 1750, 1866, 2118
HpySE526I ACGT 3 cut(s) 554, 578, 2237
HspAI GCGC 1 cut(s) 183
KpnI GGTACC 1 cut(s) 363
KspAI GTTAAC 1 cut(s) 2235
Kzo9I GATC 8 cut(s) 244, 255, 690, 1001, 1203, 1520, 1705, 1915
LmnI GCTCC 5 cut(s) 343, 588, 1360, 1571, 2053
Lsp1109I GCAGC 7 cut(s) 65, 83, 615, 691, 740, 999, 2183
LweI GCATC 5 cut(s) 12, 510, 715, 1991, 2137
MaeI CTAG 7 cut(s) 1173, 1457, 1577, 1820, 1883, 2057, 2190
MaeII ACGT 3 cut(s) 554, 578, 2237
MaeIII GTNAC 9 cut(s) 115, 139, 152, 226, 461, 550, 803, 1471, 2010
MalI GATC 8 cut(s) 246, 257, 692, 1003, 1205, 1522, 1707, 1917
MboI GATC 8 cut(s) 244, 255, 690, 1001, 1203, 1520, 1705, 1915
MboII GAAGA 6 cut(s) 991, 1113, 1226, 1561, 1925, 2123
MflI RGATCY 3 cut(s) 244, 255, 1203
MhlI GDGCHC 2 cut(s) 1576, 2124
MlsI TGGCCA 1 cut(s) 1985
MluNI TGGCCA 1 cut(s) 1985
MlyI GAGTC 4 cut(s) 482, 736, 1178, 1963
Mox20I TGGCCA 1 cut(s) 1985
MscI TGGCCA 1 cut(s) 1985
MseI TTAA 7 cut(s) 537, 594, 696, 1134, 1371, 1541, 2234
MslI CAYNNNNRTG 6 cut(s) 239, 1407, 1531, 1601, 1620, 2190
Msp20I TGGCCA 1 cut(s) 1985
MspA1I CMGCKG 1 cut(s) 56
MspCI CTTAAG 1 cut(s) 1540
MspI CCGG 4 cut(s) 323, 357, 444, 1618
MspR9I CCNGG 6 cut(s) 98, 323, 825, 924, 1201, 1619
Mva1269I GAATGC 1 cut(s) 200
MvaI CCWGG 4 cut(s) 98, 825, 924, 1201
MvnI CGCG 2 cut(s) 185, 1994
NciI CCSGG 2 cut(s) 323, 1619
NcoI CCATGG 2 cut(s) 1532, 2087
NdeII GATC 8 cut(s) 244, 255, 690, 1001, 1203, 1520, 1705, 1915
NheI GCTAGC 1 cut(s) 1172
NlaIV GGNNCC 5 cut(s) 162, 339, 361, 839, 1345
NmuCI GTSAC 4 cut(s) 115, 461, 550, 1471
NruI TCGCGA 1 cut(s) 1994
NspI RCATGY 3 cut(s) 183, 454, 2221
PaeI GCATGC 1 cut(s) 2221
PceI AGGCCT 2 cut(s) 1864, 2029
PctI GAATGC 1 cut(s) 200
PfeI GAWTC 5 cut(s) 435, 639, 973, 1696, 1924
PflFI GACNNNGTC 1 cut(s) 1946
PinAI ACCGGT 1 cut(s) 443
PkrI GCNGC 9 cut(s) 55, 64, 73, 187, 605, 681, 730, 989, 2173
PleI GAGTC 4 cut(s) 482, 736, 1178, 1963
PpsI GAGTC 4 cut(s) 482, 736, 1178, 1963
Ppu21I YACGTR 1 cut(s) 579
Psp6I CCWGG 4 cut(s) 96, 823, 922, 1199
PspFI CCCAGC 2 cut(s) 298, 1063
PspGI CCWGG 4 cut(s) 96, 823, 922, 1199
PspN4I GGNNCC 5 cut(s) 162, 339, 361, 839, 1345
PspPI GGNCC 1 cut(s) 276
PstI CTGCAG 2 cut(s) 605, 899
PsuI RGATCY 3 cut(s) 244, 255, 1203
PsyI GACNNNGTC 1 cut(s) 1946
RruI TCGCGA 1 cut(s) 1994
RsaI GTAC 4 cut(s) 264, 361, 547, 2197
RsaNI GTAC 4 cut(s) 263, 360, 546, 2196
RseI CAYNNNNRTG 6 cut(s) 239, 1407, 1531, 1601, 1620, 2190
SaqAI TTAA 7 cut(s) 537, 594, 696, 1134, 1371, 1541, 2234
SatI GCNGC 9 cut(s) 54, 63, 72, 186, 604, 680, 729, 988, 2172
Sau3AI GATC 8 cut(s) 244, 255, 690, 1001, 1203, 1520, 1705, 1915
Sau96I GGNCC 1 cut(s) 276
SchI GAGTC 4 cut(s) 482, 736, 1178, 1963
ScrFI CCNGG 6 cut(s) 98, 323, 825, 924, 1201, 1619
SduI GDGCHC 2 cut(s) 1576, 2124
SfaNI GCATC 5 cut(s) 12, 510, 715, 1991, 2137
SfcI CTRYAG 4 cut(s) 66, 601, 798, 895
SmiMI CAYNNNNRTG 6 cut(s) 239, 1407, 1531, 1601, 1620, 2190
SmlI CTYRAG 3 cut(s) 732, 1540, 2005
SmoI CTYRAG 3 cut(s) 732, 1540, 2005
SnaBI TACGTA 1 cut(s) 579
SpeI ACTAGT 1 cut(s) 1456
SphI GCATGC 1 cut(s) 2221
SseBI AGGCCT 2 cut(s) 1864, 2029
SsiI CCGC 6 cut(s) 56, 62, 108, 185, 1089, 2205
SspI AATATT 2 cut(s) 1299, 1963
SspMI CTAG 7 cut(s) 1173, 1457, 1577, 1820, 1883, 2057, 2190
StuI AGGCCT 2 cut(s) 1864, 2029
StyD4I CCNGG 6 cut(s) 96, 321, 823, 922, 1199, 1617
StyI CCWWGG 4 cut(s) 1177, 1532, 2049, 2087
TaaI ACNGT 6 cut(s) 419, 476, 533, 550, 1285, 1644
TaiI ACGT 3 cut(s) 557, 581, 2240
TaqI TCGA 1 cut(s) 1000
TatI WGTACW 1 cut(s) 262
TauI GCSGC 2 cut(s) 65, 188
TfiI GAWTC 5 cut(s) 435, 639, 973, 1696, 1924
Tru1I TTAA 7 cut(s) 537, 594, 696, 1134, 1371, 1541, 2234
Tru9I TTAA 7 cut(s) 537, 594, 696, 1134, 1371, 1541, 2234
TscAI CASTG 3 cut(s) 481, 821, 1911
TseFI GTSAC 4 cut(s) 115, 461, 550, 1471
TseI GCWGC 7 cut(s) 53, 71, 603, 679, 728, 987, 2171
Tsp45I GTSAC 4 cut(s) 115, 461, 550, 1471
TspGWI ACGGA 3 cut(s) 207, 229, 1799
TspRI CASTG 3 cut(s) 481, 821, 1911
Tth111I GACNNNGTC 1 cut(s) 1946
Vha464I CTTAAG 1 cut(s) 1540
XapI RAATTY 3 cut(s) 748, 916, 1929
XceI RCATGY 3 cut(s) 183, 454, 2221
XcmI CCANNNNNNNNNTGG 1 cut(s) 238
XspI CTAG 7 cut(s) 1173, 1457, 1577, 1820, 1883, 2057, 2190
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.