RchiOBHm_Chr5g0017041

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000066
Physical Location & Seq
Reverse (-)
11725281 .. 11728042
2762 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29736

Sequence Viewer

Length: 2094 bp
ATGCAATTCATACTGGTAGCGGTACTGGTTCTCTTCACATTTGCGGCCGCGGCCGAAGAAGCAGTAGCAGCTGAAGCCGTCCTGCCTCAAGCCAAGCCTGGCTGCACTGACCGCTGTGGCAACCTCACCATTCCATATCCATTTGGCATAGAGCCTGATTGTTACATGACAGAAAACTTTTCTGTTAGTTGTAACACATCCACTAAACCCCCGACAGCAACGTGGGAGGATAGTCCCATCGTTATTAACAACATATACCTGGCTGAAGGTGAGATGCAAATAATGCACTACGTAGCCTACGATTGCTACGACAGCCAGGGCCCGACTGAGTCCGACAAACCATTGCTCACCGTGCCTCCTTTCACCGTCTCTCATACTAGAAACAAGTTCATTGCCGTTGGTTGCGATACTTATGCACTTTTCAGAGGTTACCGTGCAGACGAAGAGAGGAACATCATGACCGGATGCATGTCCATGTGTGACAACCTTGACAGCGTTGAACAGTCTTGCTCCGGGGTTGGGTGTTGCCAAACTGACATCCCTAGAGGACTTGAGAACTGCACTCTCACACTGGCTAGCTACTATAATCATACATACATACTGGAGTTCAATCGATGCAGCTATGCCTTCTTTGTGGAAAAAGACGAGTTTACGTTTTCCCCGAATACAAGTTTTAAAGACCTCTCCAACACTGAAAAGCTTCCCATGATTCTTAATTGGGAAATTGGGGAAGGGCCATGTGATGAAGCTCAAAAGAAAGATGATTATGCATGCAAGGCAAATAGTAAGTGTGTCAATCGGACCATCAGCGATGAGCTATCTGGTTATATTTGTCAATGCCAGCCGGGTTATCAAGGGAACCCCTACCTTCCTGATGGATGCCAAGATATTGACGAGTGCAAGGTTTCAAACCCATGCACCAATGGAAGGTGTGAAAATTCACCTGGAAGTTACTCTTGTTTGTGTGACAAAGGGTACAGAAGCAATGGAACCAATAATCAAAGTTGTATTAAGGAGGTTCCTAAAAATCAACCAAAGACCACGCTCCTGCTCCTGCTTATTGTATCATTGAGTGTAACTGGCGGCTTCTTAGTTCTATTTGTTGGAATTTCATGGATATGTTGCGGAATAAAGAAAAGAAAATTCATGAAACTCAAAGAAAAGTACTTTAAAGAGAATGGTGGCTTATTGTTACTACAGAAACTCGCTAATAATGGAGGCTCAGTGGAGACAACAAAAATATTCACCACAGAAGAACTAGAGAAGGCAACAAACAATTACCATGAGAGTAGAGTCCTTGGAGAAGGAGGCTACGGAACAGTTTATAAAGGGGTACTACTTGACAACAAAGTGGTTGCCATAAAGAAGTCCAAAGGTGGTACCTCGATCCAGAGTGAACAGCTTGTTAATGAGGTGGTTGTTCTTTCCCAAATCAACCACAGAAATGTGGTGAAGCTACTAGGTTGTTGTTTAGAGACAGCAGTACCTTTACTAGTATATGAGTTTGTCACCCATGGCACTTTATATGAGCACATTCATAGAAAAAGATCACTACTGTCATTCGAACTACGAATGAAGATAGCTGTTGAAACAGCAGGAGCACTAGCATACTTACACTCCTCTATATCCACGCCAATCATACATCGAGATGTGAAAGCAGCAAATATACTCCTAGATGATAATTATACAGCTAAAGTGTCGGACTTTGGAGCTTCACGGTTGGTTCCTTCAGGTCAAAATGAAATACAAACTTTAGTGCTCGGGACATTTGGCTACCTAGACCCAGAATATTTGCAGTCAAACCAACTAACAGAAAAAAGTGATGTCTACAGCTTTGGAGTTGTCCTAGTGGAGCTACTAACAAGCAAAGTGGCATTTTCTAATGATAGATGCTTAGCAAGCATCTTTGTTTCTTCCATGGATGAAGATTGCTTAAAACAAATTCTTGATGATGCCATAGTGAATGAGGGAAACATTGAGACAGTAGAAAACGTGGCGAGTCTCGCAAAAAGATGTTTGAGGGTAAAAGGGGAGGAAAGGCCTACCATGAAAGAAGTTGCCATGGAGTTAGAGGGAATGACAATTACGGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK

Protein Analysis

697

Amino Acids

77.05

Weight (kDa)

5.1

Isoelectric Point (pI)

38.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 35 - 94 1.5e-09 Wall-associated receptor kinase galacturonan-binding
EGF_CA PF07645 296 - 336 1.6e-07 Calcium-binding EGF domain
Pkinase PF00069 427 - 686 4.1e-51 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 430 - 690 2e-49 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21210 AT1G21230 AT1G21240 AT1G21240 AT1G21245 AT1G21250 AT1G21270 AT1G22720 AT3G25490
fragaria_vesca FvH4_2g35051 FvH4_2g35051 FvH4_2g35060 FvH4_3g10281 FvH4_3g10285 FvH4_3g10286 FvH4_3g10311 FvH4_3g10311 FvH4_3g10320 FvH4_3g10332 FvH4_3g10340 FvH4_3g10350 FvH4_3g10360 FvH4_3g10390 FvH4_3g10412 FvH4_3g10413 FvH4_3g10461 FvH4_3g10462 FvH4_3g10470 FvH4_3g10510 FvH4_3g10520 FvH4_3g10530 FvH4_3g10550 FvH4_3g10560 FvH4_3g10611 FvH4_3g11730 FvH4_6g53140 FvH4_6g53142 FvH4_7g31880 FvH4_7g31910 FvH4_7g31920
malus_domestica MD05G1269900.v1.1 MD05G1270000.v1.1 MD05G1270200.v1.1 MD05G1270400.v1.1 MD05G1270500.v1.1 MD05G1270600.v1.1 MD05G1270800.v1.1 MD05G1270900.v1.1 MD07G1183800.v1.1 MD08G1107600.v1.1 MD10G1248500.v1.1 MD10G1249200.v1.1 MD10G1249500.v1.1 MD10G1250000.v1.1 MD10G1250500.v1.1 MD10G1250900.v1.1 MD10G1251200.v1.1 MD10G1251400.v1.1 MD13G1004600.v1.1 MD15G1088400.v1.1
prunus_persica Prupe.1G442400_v2.0.a1 Prupe.1G442500_v2.0.a1 Prupe.2G223400_v2.0.a1 Prupe.4G091900_v2.0.a1 Prupe.4G093200_v2.0.a1 Prupe.4G093300_v2.0.a1 Prupe.4G093400_v2.0.a1 Prupe.4G093500_v2.0.a1 Prupe.4G094100_v2.0.a1 Prupe.4G094200_v2.0.a1 Prupe.4G094300_v2.0.a1 Prupe.6G358300_v2.0.a1
pyrus_communis pycom08g08990 pycom10g20660 pycom10g20670 pycom10g20680 pycom10g20710 pycom10g20740 pycom13g00400 pycom15g08290
rosa_chinensis RchiOBHm_Chr1g0326861 RchiOBHm_Chr1g0326871 RchiOBHm_Chr1g0331391 RchiOBHm_Chr1g0336911 RchiOBHm_Chr1g0364001 RchiOBHm_Chr2g0174541 RchiOBHm_Chr2g0175041 RchiOBHm_Chr2g0175101 RchiOBHm_Chr2g0175121 RchiOBHm_Chr3g0486331 RchiOBHm_Chr5g0015581 RchiOBHm_Chr5g0016551 RchiOBHm_Chr5g0016561 RchiOBHm_Chr5g0016581 RchiOBHm_Chr5g0016601 RchiOBHm_Chr5g0016611 RchiOBHm_Chr5g0016641 RchiOBHm_Chr5g0016661 RchiOBHm_Chr5g0016691 RchiOBHm_Chr5g0016701 RchiOBHm_Chr5g0016731 RchiOBHm_Chr5g0016741 RchiOBHm_Chr5g0016811 RchiOBHm_Chr5g0016841 RchiOBHm_Chr5g0016871 RchiOBHm_Chr5g0016881 RchiOBHm_Chr5g0016931 RchiOBHm_Chr5g0017021 RchiOBHm_Chr5g0017041 RchiOBHm_Chr5g0017071 RchiOBHm_Chr5g0017091 RchiOBHm_Chr5g0017121 RchiOBHm_Chr5g0017141 RchiOBHm_Chr5g0017291 RchiOBHm_Chr5g0017321 RchiOBHm_Chr5g0017401 RchiOBHm_Chr5g0019001 RchiOBHm_Chr5g0019071 RchiOBHm_Chr5g0028941 RchiOBHm_Chr5g0052141 RchiOBHm_Chr5g0054721 RchiOBHm_Chr5g0054801 RchiOBHm_Chr5g0054931 RchiOBHm_Chr5g0054941 RchiOBHm_Chr6g0259291 RchiOBHm_Chr6g0292991 RchiOBHm_Chr6g0306911
rosa_laevigata RLG00000009465 RLG00000009468 RLG00000010755 RLG00000011976 RLG00000014541 RLG00000014542 RLG00000015169 RLG00000022308 RLG00000027529 RLG00000029324 RLG00000030244 RLG00000030245 RLG00000032210 RLG00000032211 RLG00000032257 RLG00000032259 RLG00000032260 RLG00000032261 RLG00000032262 RLG00000032268 RLG00000032269 RLG00000032272 RLG00000032282 RLG00000032283 RLG00000032308 RLG00000032413 RLG00000033129 RLG00000034945
rosa_multiflora Rmu_co8241929.1_g000001 Rmu_co8269227.1_g000001 Rmu_co8461259.1_g000001 Rmu_co8495385.1_g000001 Rmu_sc0000028.1_g000003 Rmu_sc0000083.1_g000009 Rmu_sc0000083.1_g000010 Rmu_sc0000083.1_g000031 Rmu_sc0000083.1_g000043 Rmu_sc0000083.1_g000046 Rmu_sc0000083.1_g000047 Rmu_sc0000563.1_g000024 Rmu_sc0000842.1_g000012 Rmu_sc0001235.1_g000001 Rmu_sc0001235.1_g000005 Rmu_sc0001235.1_g000009 Rmu_sc0001235.1_g000015 Rmu_sc0001235.1_g000016 Rmu_sc0001235.1_g000017 Rmu_sc0001473.1_g000036 Rmu_sc0001607.1_g000016 Rmu_sc0001788.1_g000006 Rmu_sc0002018.1_g000008 Rmu_sc0002028.1_g000001 Rmu_sc0002680.1_g000011 Rmu_sc0002798.1_g000023 Rmu_sc0002886.1_g000023 Rmu_sc0002989.1_g000012 Rmu_sc0003064.1_g000009 Rmu_sc0003382.1_g000006 Rmu_sc0003630.1_g000002 Rmu_sc0003630.1_g000047 Rmu_sc0003630.1_g000066 Rmu_sc0003630.1_g000067 Rmu_sc0003649.1_g000010 Rmu_sc0003688.1_g000005 Rmu_sc0003920.1_g000007 Rmu_sc0004765.1_g000007 Rmu_sc0004765.1_g000008 Rmu_sc0004920.1_g000011 Rmu_sc0005223.1_g000008 Rmu_sc0005223.1_g000010 Rmu_sc0005223.1_g000015 Rmu_sc0005223.1_g000016 Rmu_sc0005319.1_g000014 Rmu_sc0005319.1_g000015 Rmu_sc0005319.1_g000018 Rmu_sc0005319.1_g000019 Rmu_sc0005319.1_g000025 Rmu_sc0005418.1_g000007 Rmu_sc0005418.1_g000012 Rmu_sc0006106.1_g000011 Rmu_sc0006914.1_g000012 Rmu_sc0007023.1_g000001 Rmu_sc0007662.1_g000001 Rmu_sc0007920.1_g000002 Rmu_sc0007920.1_g000013 Rmu_sc0008053.1_g000016 Rmu_sc0008053.1_g000017 Rmu_sc0008053.1_g000018 Rmu_sc0008053.1_g000023 Rmu_sc0008053.1_g000050 Rmu_sc0009042.1_g000007 Rmu_sc0009042.1_g000008 Rmu_sc0010642.1_g000008 Rmu_sc0011109.1_g000002 Rmu_sc0011314.1_g000006 Rmu_sc0011974.1_g000006 Rmu_sc0012179.1_g000009 Rmu_sc0015169.1_g000001 Rmu_sc0016368.1_g000002 Rmu_sc0022659.1_g000001 Rmu_sc0027371.1_g000001 Rmu_sc0028213.1_g000001 Rmu_sc0034526.1_g000001 Rmu_sc0039366.1_g000002 Rmu_ssc0000050.1_g000084 Rmu_ssc0000050.1_g000088 Rmu_ssc0000167.1_g000017
rosa_roxburghii Rroxscaffold_1G00011340 Rroxscaffold_1G00025710 Rroxscaffold_1G00025870 Rroxscaffold_1G00026070 Rroxscaffold_1G00026170 Rroxscaffold_1G00026200 Rroxscaffold_1G00026270 Rroxscaffold_1G00050950 Rroxscaffold_1G00058900 Rroxscaffold_1G00060040 Rroxscaffold_1G00060060 Rroxscaffold_1G00060070 Rroxscaffold_1G00060080 Rroxscaffold_1G00060110 Rroxscaffold_1G00060120 Rroxscaffold_1G00060140 Rroxscaffold_1G00060170 Rroxscaffold_1G00060210 Rroxscaffold_1G00060220 Rroxscaffold_1G00060240 Rroxscaffold_1G00060250 Rroxscaffold_1G00060260 Rroxscaffold_1G00060300 Rroxscaffold_1G00060320 Rroxscaffold_1G00060330 Rroxscaffold_1G00060900 Rroxscaffold_1G00060920 Rroxscaffold_1G00073980 Rroxscaffold_2G00077560 Rroxscaffold_2G00077580 Rroxscaffold_2G00146990 Rroxscaffold_3G00236200 Rroxscaffold_3G00236210 Rroxscaffold_3G00236220 Rroxscaffold_4G00292760 Rroxscaffold_4G00315200 Rroxscaffold_4G00315240 Rroxscaffold_4G00326240 Rroxscaffold_4G00328980 Rroxscaffold_5G00341890 Rroxscaffold_7G00161350 Rroxscaffold_7G00174490 Rroxscaffold_7G00206550
rosa_rugosa Rorug01G0093000 Rorug01G0093100 Rorug01G0128800 Rorug01G0312000 Rorug01G0312000 Rorug02G0581100 Rorug03G0224600 Rorug05G0028500 Rorug05G0032700 Rorug05G0032800 Rorug05G0032900 Rorug05G0032900 Rorug05G0033000 Rorug05G0033100 Rorug05G0033300 Rorug05G0033400 Rorug05G0033900 Rorug05G0034900 Rorug05G0035000 Rorug05G0046900 Rorug05G0047500 Rorug05G0047500 Rorug05G0111600 Rorug05G0287900 Rorug05G0288700 Rorug05G0586900 Rorug06G0231700 Rorug06G0354300 Rorug06G0354400
rosa_samantha Rh1DG122200 Rh2DG686800 Rh5BG136600 Rh6AG344500 Rh6BG351900 Rh6CG359000 Rh6CG359100 Rh6CG387600 Rh6CG480500 Rh6DG344600 Rh6DG467400
rosa_wichuraiana Rw0G010250 Rw0G014820 Rw0G021400 Rw0G023100 Rw1G009450 Rw1G012100 Rw1G012180 Rw1G028360 Rw2G054260 Rw3G024370 Rw3G024420 Rw3G024470 Rw5G010850 Rw5G011010 Rw5G011020 Rw5G011050 Rw5G011080 Rw5G011120 Rw5G012300 Rw5G018470 Rw5G033830 Rw6G008930 Rw6G030070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1326
Acc65I GGTACC 1 cut(s) 1379
AccB1I GGYRCC 1 cut(s) 1379
AccI GTMKAC 1 cut(s) 1827
AccII CGCG 1 cut(s) 50
AciI CCGC 7 cut(s) 20, 44, 48, 50, 112, 1083, 1125
AclWI GGATC 1 cut(s) 1381
AcoI YGGCCR 2 cut(s) 45, 51
AcsI RAATTY 4 cut(s) 937, 1107, 1142, 1941
AcuI CTGAAG 3 cut(s) 93, 285, 1713
AfaI GTAC 6 cut(s) 24, 977, 1166, 1335, 1381, 1485
AfiI CCNNNNNNNGG 2 cut(s) 519, 927
AgsI TTSAA 4 cut(s) 500, 610, 909, 1589
AhlI ACTAGT 1 cut(s) 1492
AjnI CCWGG 4 cut(s) 97, 258, 315, 943
Alw21I GWGCWC 3 cut(s) 1533, 1603, 1761
Alw26I GTCTC 5 cut(s) 373, 1223, 1469, 1973, 2006
AlwI GGATC 1 cut(s) 1381
Ama87I CYCGRG 1 cut(s) 1760
AoxI GGCC 5 cut(s) 45, 51, 319, 734, 2039
ApaI GGGCCC 1 cut(s) 323
ApeKI GCWGC 4 cut(s) 68, 102, 618, 1658
ApoI RAATTY 4 cut(s) 937, 1107, 1142, 1941
Asp700I GAANNNNTTC 1 cut(s) 699
Asp718I GGTACC 1 cut(s) 1379
AspS9I GGNCC 4 cut(s) 319, 320, 734, 801
AsuC2I CCSGG 2 cut(s) 514, 846
AsuHPI GGTGA 8 cut(s) 118, 281, 340, 355, 933, 1237, 1462, 1501
AsuII TTCGAA 1 cut(s) 1563
AsuNHI GCTAGC 1 cut(s) 575
AvaI CYCGRG 1 cut(s) 1760
AvaII GGWCC 1 cut(s) 801
BaeGI GKGCMC 1 cut(s) 323
BaeI ACNNNNGTAYC 2 cut(s) 1467, 1500
BanI GGYRCC 1 cut(s) 1379
BanII GRGCYC 1 cut(s) 323
Bbv12I GWGCWC 3 cut(s) 1533, 1603, 1761
BbvI GCAGC 4 cut(s) 80, 89, 630, 1670
BccI CCATC 3 cut(s) 245, 812, 869
BceAI ACGGC 2 cut(s) 62, 380
BcgI CGANNNNNNTGC 2 cut(s) 395, 429
BciT130I CCWGG 4 cut(s) 99, 260, 317, 945
BcnI CCSGG 2 cut(s) 514, 846
BcoDI GTCTC 5 cut(s) 373, 1223, 1469, 1973, 2006
BcuI ACTAGT 1 cut(s) 1492
BfmI CTRYAG 2 cut(s) 1196, 1828
BisI GCNGC 8 cut(s) 45, 48, 51, 69, 103, 619, 1084, 1659
BlpI GCTNAGC 1 cut(s) 1894
BlsI GCNGC 8 cut(s) 46, 49, 52, 70, 104, 620, 1085, 1660
BmcAI AGTACT 1 cut(s) 1166
Bme1390I CCNGG 6 cut(s) 99, 260, 317, 514, 846, 945
Bme18I GGWCC 1 cut(s) 801
BmeT110I CYCGRG 1 cut(s) 1760
BmgT120I GGNCC 4 cut(s) 319, 320, 734, 801
BmiI GGNNCC 6 cut(s) 321, 860, 991, 1020, 1381, 1725
BmrFI CCNGG 6 cut(s) 99, 260, 317, 514, 846, 945
BmsI GCATC 7 cut(s) 264, 455, 605, 869, 1880, 1911, 1942
BmtI GCTAGC 1 cut(s) 579
BpmI CTGGAG 1 cut(s) 623
Bpu1102I GCTNAGC 1 cut(s) 1894
Bpu14I TTCGAA 1 cut(s) 1563
BpuEI CTTGAG 2 cut(s) 72, 572
BpuMI CCSGG 2 cut(s) 514, 846
Bsa29I ATCGAT 1 cut(s) 613
BsaAI YACGTR 1 cut(s) 292
BsaJI CCNNGG 7 cut(s) 48, 316, 513, 1297, 1513, 1917, 2061
BsaWI WCCGGW 1 cut(s) 461
BsaXI ACNNNNNCTCC 6 cut(s) 340, 370, 1209, 1239, 1601, 1631
Bsc4I CCNNNNNNNGG 2 cut(s) 519, 927
Bse1I ACTGG 5 cut(s) 18, 30, 576, 606, 1084
Bse3DI GCAATG 3 cut(s) 341, 390, 991
BseBI CCWGG 4 cut(s) 99, 260, 317, 945
BseCI ATCGAT 1 cut(s) 613
BseDI CCNNGG 7 cut(s) 48, 316, 513, 1297, 1513, 1917, 2061
BseGI GGATG 5 cut(s) 197, 470, 537, 884, 1927
BseLI CCNNNNNNNGG 2 cut(s) 519, 927
BseMI GCAATG 3 cut(s) 341, 390, 991
BseMII CTCAG 2 cut(s) 318, 1236
BseNI ACTGG 5 cut(s) 18, 30, 576, 606, 1084
BseRI GAGGAG 1 cut(s) 1609
BseSI GKGCMC 1 cut(s) 323
BseX3I CGGCCG 2 cut(s) 45, 51
BseXI GCAGC 4 cut(s) 80, 89, 630, 1670
BsgI GTGCAG 3 cut(s) 88, 456, 544
Bsh1236I CGCG 1 cut(s) 50
Bsh1285I CGRYCG 2 cut(s) 48, 54
BshFI GGCC 5 cut(s) 47, 53, 321, 736, 2041
BshNI GGYRCC 1 cut(s) 1379
BshVI ATCGAT 1 cut(s) 613
BsiEI CGRYCG 2 cut(s) 48, 54
BsiHKAI GWGCWC 3 cut(s) 1533, 1603, 1761
BsiHKCI CYCGRG 1 cut(s) 1760
BsiSI CCGG 3 cut(s) 462, 513, 845
BslFI GGGAC 2 cut(s) 219, 1777
BslI CCNNNNNNNGG 2 cut(s) 519, 927
BsmAI GTCTC 5 cut(s) 373, 1223, 1469, 1973, 2006
BsmBI CGTCTC 1 cut(s) 373
BsmFI GGGAC 2 cut(s) 219, 1777
BsnI GGCC 5 cut(s) 47, 53, 321, 736, 2041
BsoBI CYCGRG 1 cut(s) 1760
Bsp119I TTCGAA 1 cut(s) 1563
Bsp120I GGGCCC 1 cut(s) 319
Bsp1286I GDGCHC 4 cut(s) 323, 1533, 1603, 1761
Bsp143I GATC 2 cut(s) 1386, 1547
Bsp1720I GCTNAGC 1 cut(s) 1894
Bsp19I CCATGG 3 cut(s) 1513, 1917, 2061
BspACI CCGC 7 cut(s) 20, 44, 48, 50, 112, 1083, 1125
BspANI GGCC 5 cut(s) 47, 53, 321, 736, 2041
BspCNI CTCAG 2 cut(s) 319, 1235
BspDI ATCGAT 1 cut(s) 613
BspFNI CGCG 1 cut(s) 50
BspHI TCATGA 2 cut(s) 456, 1146
BspLI GGNNCC 6 cut(s) 321, 860, 991, 1020, 1381, 1725
BspOI GCTAGC 1 cut(s) 579
BspPI GGATC 1 cut(s) 1381
BspT104I TTCGAA 1 cut(s) 1563
BspT107I GGYRCC 1 cut(s) 1379
BsrDI GCAATG 3 cut(s) 341, 390, 991
BsrI ACTGG 5 cut(s) 18, 30, 576, 606, 1084
BssECI CCNNGG 7 cut(s) 48, 316, 513, 1297, 1513, 1917, 2061
BssMI GATC 2 cut(s) 1386, 1547
BssT1I CCWWGG 4 cut(s) 1297, 1513, 1917, 2061
Bst2UI CCWGG 4 cut(s) 99, 260, 317, 945
Bst4CI ACNGT 8 cut(s) 352, 367, 434, 504, 1321, 1557, 1719, 1984
Bst6I CTCTTC 2 cut(s) 38, 438
BstAPI GCANNNNNTGC 1 cut(s) 283
BstBAI YACGTR 1 cut(s) 292
BstBI TTCGAA 1 cut(s) 1563
BstC8I GCNNGC 4 cut(s) 577, 772, 842, 1900
BstDEI CTNAG 4 cut(s) 327, 1090, 1222, 1894
BstDSI CCRYGG 4 cut(s) 48, 1513, 1917, 2061
BstEII GGTNACC 1 cut(s) 428
BstF5I GGATG 5 cut(s) 197, 470, 537, 884, 1927
BstFNI CGCG 1 cut(s) 50
BstKTI GATC 2 cut(s) 1389, 1550
BstMAI GTCTC 5 cut(s) 373, 1223, 1469, 1973, 2006
BstMBI GATC 2 cut(s) 1386, 1547
BstMCI CGRYCG 2 cut(s) 48, 54
BstNI CCWGG 4 cut(s) 99, 260, 317, 945
BstNSI RCATGY 2 cut(s) 472, 774
BstPI GGTNACC 1 cut(s) 428
BstSCI CCNGG 6 cut(s) 97, 258, 315, 512, 844, 943
BstSFI CTRYAG 2 cut(s) 1196, 1828
BstSLI GKGCMC 1 cut(s) 323
BstSNI TACGTA 1 cut(s) 292
BstUI CGCG 1 cut(s) 50
BstV1I GCAGC 4 cut(s) 80, 89, 630, 1670
BstZI CGGCCG 2 cut(s) 45, 51
Bsu15I ATCGAT 1 cut(s) 613
BsuRI GGCC 5 cut(s) 47, 53, 321, 736, 2041
BsuTUI ATCGAT 1 cut(s) 613
BtgI CCRYGG 4 cut(s) 48, 1513, 1917, 2061
BtgZI GCGATG 1 cut(s) 825
BtsCI GGATG 5 cut(s) 197, 470, 537, 884, 1927
BtsIMutI CAGTG 4 cut(s) 105, 569, 690, 1230
Cac8I GCNNGC 4 cut(s) 577, 772, 842, 1900
CciI TCATGA 2 cut(s) 456, 1146
CciNI GCGGCCGC 1 cut(s) 45
Cfr13I GGNCC 4 cut(s) 319, 320, 734, 801
Cfr42I CCGCGG 1 cut(s) 51
ClaI ATCGAT 1 cut(s) 613
Csp6I GTAC 6 cut(s) 23, 976, 1165, 1334, 1380, 1484
CspCI CAANNNNNGTGG 2 cut(s) 1851, 1886
CviQI GTAC 6 cut(s) 23, 976, 1165, 1334, 1380, 1484
DdeI CTNAG 4 cut(s) 327, 1090, 1222, 1894
DpnI GATC 2 cut(s) 1388, 1549
DpnII GATC 2 cut(s) 1386, 1547
DraI TTTAAA 2 cut(s) 676, 1171
EaeI YGGCCR 2 cut(s) 45, 51
EagI CGGCCG 2 cut(s) 45, 51
Eam1104I CTCTTC 2 cut(s) 38, 438
EarI CTCTTC 2 cut(s) 38, 438
EclXI CGGCCG 2 cut(s) 45, 51
Eco105I TACGTA 1 cut(s) 292
Eco130I CCWWGG 4 cut(s) 1297, 1513, 1917, 2061
Eco147I AGGCCT 1 cut(s) 2041
Eco24I GRGCYC 1 cut(s) 323
Eco47I GGWCC 1 cut(s) 801
Eco52I CGGCCG 2 cut(s) 45, 51
Eco57I CTGAAG 3 cut(s) 93, 285, 1713
Eco88I CYCGRG 1 cut(s) 1760
Eco91I GGTNACC 1 cut(s) 428
EcoO109I RGGNCCY 1 cut(s) 319
EcoO65I GGTNACC 1 cut(s) 428
EcoRII CCWGG 4 cut(s) 97, 258, 315, 943
EcoT14I CCWWGG 4 cut(s) 1297, 1513, 1917, 2061
EcoT22I ATGCAT 2 cut(s) 470, 772
EcoT38I GRGCYC 1 cut(s) 323
ErhI CCWWGG 4 cut(s) 1297, 1513, 1917, 2061
Esp3I CGTCTC 1 cut(s) 373
FalI AAGNNNNNCTT 2 cut(s) 940, 972
FaqI GGGAC 2 cut(s) 219, 1777
FblI GTMKAC 1 cut(s) 1827
Fnu4HI GCNGC 8 cut(s) 45, 48, 51, 69, 103, 619, 1084, 1659
FokI GGATG 5 cut(s) 184, 477, 524, 891, 1934
FriOI GRGCYC 1 cut(s) 323
Fsp4HI GCNGC 8 cut(s) 45, 48, 51, 69, 103, 619, 1084, 1659
GluI GCNGC 8 cut(s) 45, 48, 51, 69, 103, 619, 1084, 1659
GsuI CTGGAG 1 cut(s) 623
HaeIII GGCC 5 cut(s) 47, 53, 321, 736, 2041
HapII CCGG 3 cut(s) 462, 513, 845
HindIII AAGCTT 1 cut(s) 698
HinfI GANTC 4 cut(s) 329, 709, 1293, 1999
HpaII CCGG 3 cut(s) 462, 513, 845
HphI GGTGA 8 cut(s) 118, 281, 340, 355, 933, 1237, 1462, 1501
Hpy166II GTNNAC 3 cut(s) 651, 1397, 1828
Hpy188I TCNGA 4 cut(s) 334, 425, 801, 1702
Hpy188III TCNNGA 7 cut(s) 457, 872, 1147, 1390, 1646, 1762, 1946
Hpy8I GTNNAC 3 cut(s) 651, 1397, 1828
HpyAV CCTTC 8 cut(s) 260, 637, 725, 878, 921, 1258, 1298, 1737
HpyCH4III ACNGT 8 cut(s) 352, 367, 434, 504, 1321, 1557, 1719, 1984
HpyCH4IV ACGT 4 cut(s) 221, 291, 653, 1992
HpyF3I CTNAG 4 cut(s) 327, 1090, 1222, 1894
HpySE526I ACGT 4 cut(s) 221, 291, 653, 1992
KpnI GGTACC 1 cut(s) 1383
KspI CCGCGG 1 cut(s) 51
Kzo9I GATC 2 cut(s) 1386, 1547
LmnI GCTCC 6 cut(s) 515, 1050, 1056, 1598, 1709, 1852
Lsp1109I GCAGC 4 cut(s) 80, 89, 630, 1670
LweI GCATC 7 cut(s) 264, 455, 605, 869, 1880, 1911, 1942
MaeII ACGT 4 cut(s) 221, 291, 653, 1992
MaeIII GTNAC 9 cut(s) 161, 191, 428, 479, 950, 965, 1075, 1191, 1507
MalI GATC 2 cut(s) 1388, 1549
MboI GATC 2 cut(s) 1386, 1547
MboII GAAGA 7 cut(s) 25, 68, 455, 1265, 1588, 1905, 1937
MhlI GDGCHC 4 cut(s) 323, 1533, 1603, 1761
MlyI GAGTC 3 cut(s) 338, 1302, 2008
MmeI TCCRAC 4 cut(s) 357, 711, 1084, 1680
Mph1103I ATGCAT 2 cut(s) 470, 772
MroXI GAANNNNTTC 1 cut(s) 699
MseI TTAA 7 cut(s) 246, 675, 714, 1011, 1170, 1407, 1934
MslI CAYNNNNRTG 4 cut(s) 473, 1117, 1443, 1647
MspA1I CMGCKG 3 cut(s) 50, 71, 114
MspI CCGG 3 cut(s) 462, 513, 845
MspR9I CCNGG 6 cut(s) 99, 260, 317, 514, 846, 945
MvaI CCWGG 4 cut(s) 99, 260, 317, 945
MvnI CGCG 1 cut(s) 50
NciI CCSGG 2 cut(s) 514, 846
NcoI CCATGG 3 cut(s) 1513, 1917, 2061
NdeII GATC 2 cut(s) 1386, 1547
NheI GCTAGC 1 cut(s) 575
NlaIV GGNNCC 6 cut(s) 321, 860, 991, 1020, 1381, 1725
NmuCI GTSAC 3 cut(s) 479, 965, 1507
NotI GCGGCCGC 1 cut(s) 45
NsiI ATGCAT 2 cut(s) 470, 772
NspI RCATGY 2 cut(s) 472, 774
NspV TTCGAA 1 cut(s) 1563
PaeI GCATGC 1 cut(s) 774
PagI TCATGA 2 cut(s) 456, 1146
PceI AGGCCT 1 cut(s) 2041
PcsI WCGNNNNNNNCGW 3 cut(s) 297, 306, 659
PdmI GAANNNNTTC 1 cut(s) 699
PfeI GAWTC 1 cut(s) 709
PflFI GACNNNGTC 1 cut(s) 328
PkrI GCNGC 8 cut(s) 46, 49, 52, 70, 104, 620, 1085, 1660
PleI GAGTC 3 cut(s) 337, 1301, 2007
PpsI GAGTC 3 cut(s) 337, 1301, 2007
Ppu21I YACGTR 1 cut(s) 292
PsiI TTATAA 1 cut(s) 1326
Psp6I CCWGG 4 cut(s) 97, 258, 315, 943
PspEI GGTNACC 1 cut(s) 428
PspGI CCWGG 4 cut(s) 97, 258, 315, 943
PspN4I GGNNCC 6 cut(s) 321, 860, 991, 1020, 1381, 1725
PspOMI GGGCCC 1 cut(s) 319
PspPI GGNCC 4 cut(s) 319, 320, 734, 801
PsyI GACNNNGTC 1 cut(s) 328
PvuII CAGCTG 1 cut(s) 71
RsaI GTAC 6 cut(s) 24, 977, 1166, 1335, 1381, 1485
RsaNI GTAC 6 cut(s) 23, 976, 1165, 1334, 1380, 1484
RseI CAYNNNNRTG 4 cut(s) 473, 1117, 1443, 1647
SacII CCGCGG 1 cut(s) 51
SaqAI TTAA 7 cut(s) 246, 675, 714, 1011, 1170, 1407, 1934
SatI GCNGC 8 cut(s) 45, 48, 51, 69, 103, 619, 1084, 1659
Sau3AI GATC 2 cut(s) 1386, 1547
Sau96I GGNCC 4 cut(s) 319, 320, 734, 801
ScaI AGTACT 1 cut(s) 1166
SchI GAGTC 3 cut(s) 338, 1302, 2008
ScrFI CCNGG 6 cut(s) 99, 260, 317, 514, 846, 945
SduI GDGCHC 4 cut(s) 323, 1533, 1603, 1761
SfaNI GCATC 7 cut(s) 264, 455, 605, 869, 1880, 1911, 1942
SfcI CTRYAG 2 cut(s) 1196, 1828
Sfr303I CCGCGG 1 cut(s) 51
SfuI TTCGAA 1 cut(s) 1563
SgrBI CCGCGG 1 cut(s) 51
SinI GGWCC 1 cut(s) 801
SmiMI CAYNNNNRTG 4 cut(s) 473, 1117, 1443, 1647
SmlI CTYRAG 2 cut(s) 87, 551
SmoI CTYRAG 2 cut(s) 87, 551
SnaBI TACGTA 1 cut(s) 292
SpeI ACTAGT 1 cut(s) 1492
SphI GCATGC 1 cut(s) 774
SseBI AGGCCT 1 cut(s) 2041
SsiI CCGC 7 cut(s) 20, 44, 48, 50, 112, 1083, 1125
SspI AATATT 2 cut(s) 1242, 1790
StuI AGGCCT 1 cut(s) 2041
StyD4I CCNGG 6 cut(s) 97, 258, 315, 512, 844, 943
StyI CCWWGG 4 cut(s) 1297, 1513, 1917, 2061
TaaI ACNGT 8 cut(s) 352, 367, 434, 504, 1321, 1557, 1719, 1984
TaiI ACGT 4 cut(s) 224, 294, 656, 1995
TaqI TCGA 4 cut(s) 613, 1385, 1563, 1645
TatI WGTACW 1 cut(s) 1164
TauI GCSGC 4 cut(s) 47, 50, 53, 1086
TfiI GAWTC 1 cut(s) 709
Tru1I TTAA 7 cut(s) 246, 675, 714, 1011, 1170, 1407, 1934
Tru9I TTAA 7 cut(s) 246, 675, 714, 1011, 1170, 1407, 1934
TscAI CASTG 4 cut(s) 112, 576, 697, 1230
TseFI GTSAC 3 cut(s) 479, 965, 1507
TseI GCWGC 4 cut(s) 68, 102, 618, 1658
Tsp45I GTSAC 3 cut(s) 479, 965, 1507
TspGWI ACGGA 1 cut(s) 1329
TspRI CASTG 4 cut(s) 112, 576, 697, 1230
Tth111I GACNNNGTC 1 cut(s) 328
VpaK11BI GGWCC 1 cut(s) 801
XapI RAATTY 4 cut(s) 937, 1107, 1142, 1941
XceI RCATGY 2 cut(s) 472, 774
XmiI GTMKAC 1 cut(s) 1827
XmnI GAANNNNTTC 1 cut(s) 699
ZrmI AGTACT 1 cut(s) 1166
Zsp2I ATGCAT 2 cut(s) 470, 772
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.