RchiOBHm_Chr5g0016581

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000066
Physical Location & Seq
Reverse (-)
11395687 .. 11399039
3353 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29695

Sequence Viewer

Length: 2247 bp
ATGGCGTTGCGACTCTCTTTGGTGGCAGTCCTACTCCTGTTCTCGGCGGCTAGTACTACTACGACAACAGCAGCTGCTGCTCAAGCCCTCCCAGCTGACTGCCCAGACAAGTGCGGCAATCTCACAATTCCATATCCATTTGGCATGAAGAAGGGTTGTTACCTGCGAAAAGAATTCTTTATCAATTGTAAGAATACCACCCAAGGACCAACAGCATATTTGATGGAAGGGAATATCATTGTTACTAACATTTCCCTTGATGACGGTGAGTTGCAAATATTGGCCAACGTAGCTGAAGATTGCTATAACGCCGAGGGCAATAGGACGTCCAGCTTCCGTTCTTGGCTCGAGCTGCCTCTTCCTTACACGATCTCCGATTCCAAAAACAAGTTCTACGCCGTTGGTTGTGACACGTATGCAACCATGCACGGCTTACTATGGGAAGATGAATTCATTACTGGGTGCGTGTCAGTATGCTACACCCGCGGCAGTGTTGATAAGAAGTCTTGCTCTGGCGCTGGGTGTTGCCAGACTAACATCCCCAGTGGATTGTACAATCTCACTGTGAGGTTAAATAGCTACTTCAAACATTCGTATGTGCTGGACTTTAACCCCTGCAGCTACGCCTTCATTGCAGAGCAAGGGAAGTTCAATTTCTCCCCTACCAGTTTTGAACAACTGAATGGCACCGAAGGGCTTCCAATGGTTCTTAATTGGGCCATTGGGAATGAAAGAGACGCTTGTGATGGAGCTCAGAAAAGGAAGGATTTAGCATGCAGAGGTAATTATAGCATGTGCATAAACCGGCCTATCAATGAGTCAGGAGGTTACTTGTGCCGGTGCCTGCCTGATTATGAAGGAAACCCTTACCATCCAGATGGTTGCCAAGATATTGATGAGTGCAAGACTTCAAACCCCTGCCAGAATGGAAAGTGCTCAAATTCTCCTCCAGGAAACTACTCTTGTTCATGTCACAAAGGATACAAACACGACGGCATGAATGACAGGATCTGCATCAAAAAAACTCTCAAAGATGGAAGTCATGCTTATTGTGTAAGTGCAGGCTTCTTGGTTTCATTCATTGGAATTGCATGGTTATGGTGGGGAAATAAAAAAAGAAAATTCATCAAACTGAAAGAAAAATACTTCCAAGAAAATGGAGGATTATTGTTACAACAACAGCTTGCCAGTCATGGAGGCGCCGTGGACACAACAAAAATTTTTAGCGCAGAAGAACTTCAGGAGGCTACAAACAATTTCCATGAAAGTAGAGTTCTTGGCAAAGGAGGCTATGGAATAGTATACAAAGGTATACTGCCAGATAACCAAGTGATTGCCATAAAGAAGTCAAAAGGTTGTGTCGGCAGTCAGAGTGAGCAATTTGTCAACGAGGTAATCATTCTTTCTCAAATCAACCACAGAAATGTTGTGAAGCTTTTGGGTTGTTGTTTAGAAACAGAAGTGCCTATACTTGTATACGAGTTCATCAGCCATGGCACTCTTCATGAGCACATTCATAAGAAAAGATCATCACTCTCATTTGAATTACGATTGAAGATAGCAGCTGAAGCAGCAGGAGCACTAGCCCATTTACACTCCTCAATTTCCACACCAATCATACATCGAGATGTCAAAGCAGCGAATATTCTGTTAGATGATAAGTATACGACGAAAGTGTCAGACTTTGGAGCTTCACGATTGGTTGCTTCAGGTGAAGCTGGAATACAAACTGTGGTGCTCGGGACAGTAGGATACCTGGACCCTGAATATCTGCATTCAAACCAACTAACAGAAAAGAGTGACGTTTACAGCTTTGGGGTTCTCTTGGCTGAGCTTTTAACAAGCAAAGTGGCAGTTTCTAAAGATGAATTCTTAGCAAGCATCTTTGTTGCTTCCATGGAAAAAGATTGCTTAAATCAAATGCTTGATGATGAGATAGTGAATGAAGGAACTATTGAGATGGTGAAAAATGTGGCCGAACTTGCAAGAAGATGTTTGAGGGTAAAAGGGGAGGAAAGGCCTACCATGAGAGAAGTAGCCATGGAGCTAGAAGGAATGATTATGACAAAGCATCCATGGGGTTCTGCAGATCATTACTTTCCAGAAGAGACAGAGCACTTGCTCGGATCACCCAATACTTCCAAGGATTACTCTGTGAATGTTGATGGTGGTGGTGGTCCTGGTACTGCCAGTGGGTCTAGCATGCAAATAGACATGTCAATGTCTTCATATGCTGATGGGCGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK

Protein Analysis

748

Amino Acids

82.12

Weight (kDa)

5.96

Isoelectric Point (pI)

33.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 34 - 93 6.6e-11 Wall-associated receptor kinase galacturonan-binding
Pkinase PF00069 420 - 679 1.7e-47 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 423 - 683 9.2e-49 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21210 AT1G21230 AT1G21240 AT1G21240 AT1G21245 AT1G21250 AT1G21270 AT1G22720 AT3G25490
fragaria_vesca FvH4_2g35051 FvH4_2g35051 FvH4_2g35060 FvH4_3g10281 FvH4_3g10285 FvH4_3g10286 FvH4_3g10311 FvH4_3g10311 FvH4_3g10320 FvH4_3g10332 FvH4_3g10340 FvH4_3g10350 FvH4_3g10360 FvH4_3g10390 FvH4_3g10412 FvH4_3g10413 FvH4_3g10461 FvH4_3g10462 FvH4_3g10470 FvH4_3g10510 FvH4_3g10520 FvH4_3g10530 FvH4_3g10550 FvH4_3g10560 FvH4_3g10611 FvH4_3g11730 FvH4_6g53140 FvH4_6g53142 FvH4_7g31880 FvH4_7g31910 FvH4_7g31920
malus_domestica MD05G1269900.v1.1 MD05G1270000.v1.1 MD05G1270200.v1.1 MD05G1270400.v1.1 MD05G1270500.v1.1 MD05G1270600.v1.1 MD05G1270800.v1.1 MD05G1270900.v1.1 MD07G1183800.v1.1 MD08G1107600.v1.1 MD10G1248500.v1.1 MD10G1249200.v1.1 MD10G1249500.v1.1 MD10G1250000.v1.1 MD10G1250500.v1.1 MD10G1250900.v1.1 MD10G1251200.v1.1 MD10G1251400.v1.1 MD13G1004600.v1.1 MD15G1088400.v1.1
prunus_persica Prupe.1G442400_v2.0.a1 Prupe.1G442500_v2.0.a1 Prupe.2G223400_v2.0.a1 Prupe.4G091900_v2.0.a1 Prupe.4G093200_v2.0.a1 Prupe.4G093300_v2.0.a1 Prupe.4G093400_v2.0.a1 Prupe.4G093500_v2.0.a1 Prupe.4G094100_v2.0.a1 Prupe.4G094200_v2.0.a1 Prupe.4G094300_v2.0.a1 Prupe.6G358300_v2.0.a1
pyrus_communis pycom08g08990 pycom10g20660 pycom10g20670 pycom10g20680 pycom10g20710 pycom10g20740 pycom13g00400 pycom15g08290
rosa_chinensis RchiOBHm_Chr1g0326861 RchiOBHm_Chr1g0326871 RchiOBHm_Chr1g0331391 RchiOBHm_Chr1g0336911 RchiOBHm_Chr1g0364001 RchiOBHm_Chr2g0174541 RchiOBHm_Chr2g0175041 RchiOBHm_Chr2g0175101 RchiOBHm_Chr2g0175121 RchiOBHm_Chr3g0486331 RchiOBHm_Chr5g0015581 RchiOBHm_Chr5g0016551 RchiOBHm_Chr5g0016561 RchiOBHm_Chr5g0016581 RchiOBHm_Chr5g0016601 RchiOBHm_Chr5g0016611 RchiOBHm_Chr5g0016641 RchiOBHm_Chr5g0016661 RchiOBHm_Chr5g0016691 RchiOBHm_Chr5g0016701 RchiOBHm_Chr5g0016731 RchiOBHm_Chr5g0016741 RchiOBHm_Chr5g0016811 RchiOBHm_Chr5g0016841 RchiOBHm_Chr5g0016871 RchiOBHm_Chr5g0016881 RchiOBHm_Chr5g0016931 RchiOBHm_Chr5g0017021 RchiOBHm_Chr5g0017041 RchiOBHm_Chr5g0017071 RchiOBHm_Chr5g0017091 RchiOBHm_Chr5g0017121 RchiOBHm_Chr5g0017141 RchiOBHm_Chr5g0017291 RchiOBHm_Chr5g0017321 RchiOBHm_Chr5g0017401 RchiOBHm_Chr5g0019001 RchiOBHm_Chr5g0019071 RchiOBHm_Chr5g0028941 RchiOBHm_Chr5g0052141 RchiOBHm_Chr5g0054721 RchiOBHm_Chr5g0054801 RchiOBHm_Chr5g0054931 RchiOBHm_Chr5g0054941 RchiOBHm_Chr6g0259291 RchiOBHm_Chr6g0292991 RchiOBHm_Chr6g0306911
rosa_laevigata RLG00000009465 RLG00000009468 RLG00000010755 RLG00000011976 RLG00000014541 RLG00000014542 RLG00000015169 RLG00000022308 RLG00000027529 RLG00000029324 RLG00000030244 RLG00000030245 RLG00000032210 RLG00000032211 RLG00000032257 RLG00000032259 RLG00000032260 RLG00000032261 RLG00000032262 RLG00000032268 RLG00000032269 RLG00000032272 RLG00000032282 RLG00000032283 RLG00000032308 RLG00000032413 RLG00000033129 RLG00000034945
rosa_multiflora Rmu_co8241929.1_g000001 Rmu_co8269227.1_g000001 Rmu_co8461259.1_g000001 Rmu_co8495385.1_g000001 Rmu_sc0000028.1_g000003 Rmu_sc0000083.1_g000009 Rmu_sc0000083.1_g000010 Rmu_sc0000083.1_g000031 Rmu_sc0000083.1_g000043 Rmu_sc0000083.1_g000046 Rmu_sc0000083.1_g000047 Rmu_sc0000563.1_g000024 Rmu_sc0000842.1_g000012 Rmu_sc0001235.1_g000001 Rmu_sc0001235.1_g000005 Rmu_sc0001235.1_g000009 Rmu_sc0001235.1_g000015 Rmu_sc0001235.1_g000016 Rmu_sc0001235.1_g000017 Rmu_sc0001473.1_g000036 Rmu_sc0001607.1_g000016 Rmu_sc0001788.1_g000006 Rmu_sc0002018.1_g000008 Rmu_sc0002028.1_g000001 Rmu_sc0002680.1_g000011 Rmu_sc0002798.1_g000023 Rmu_sc0002886.1_g000023 Rmu_sc0002989.1_g000012 Rmu_sc0003064.1_g000009 Rmu_sc0003382.1_g000006 Rmu_sc0003630.1_g000002 Rmu_sc0003630.1_g000047 Rmu_sc0003630.1_g000066 Rmu_sc0003630.1_g000067 Rmu_sc0003649.1_g000010 Rmu_sc0003688.1_g000005 Rmu_sc0003920.1_g000007 Rmu_sc0004765.1_g000007 Rmu_sc0004765.1_g000008 Rmu_sc0004920.1_g000011 Rmu_sc0005223.1_g000008 Rmu_sc0005223.1_g000010 Rmu_sc0005223.1_g000015 Rmu_sc0005223.1_g000016 Rmu_sc0005319.1_g000014 Rmu_sc0005319.1_g000015 Rmu_sc0005319.1_g000018 Rmu_sc0005319.1_g000019 Rmu_sc0005319.1_g000025 Rmu_sc0005418.1_g000007 Rmu_sc0005418.1_g000012 Rmu_sc0006106.1_g000011 Rmu_sc0006914.1_g000012 Rmu_sc0007023.1_g000001 Rmu_sc0007662.1_g000001 Rmu_sc0007920.1_g000002 Rmu_sc0007920.1_g000013 Rmu_sc0008053.1_g000016 Rmu_sc0008053.1_g000017 Rmu_sc0008053.1_g000018 Rmu_sc0008053.1_g000023 Rmu_sc0008053.1_g000050 Rmu_sc0009042.1_g000007 Rmu_sc0009042.1_g000008 Rmu_sc0010642.1_g000008 Rmu_sc0011109.1_g000002 Rmu_sc0011314.1_g000006 Rmu_sc0011974.1_g000006 Rmu_sc0012179.1_g000009 Rmu_sc0015169.1_g000001 Rmu_sc0016368.1_g000002 Rmu_sc0022659.1_g000001 Rmu_sc0027371.1_g000001 Rmu_sc0028213.1_g000001 Rmu_sc0034526.1_g000001 Rmu_sc0039366.1_g000002 Rmu_ssc0000050.1_g000084 Rmu_ssc0000050.1_g000088 Rmu_ssc0000167.1_g000017
rosa_roxburghii Rroxscaffold_1G00011340 Rroxscaffold_1G00025710 Rroxscaffold_1G00025870 Rroxscaffold_1G00026070 Rroxscaffold_1G00026170 Rroxscaffold_1G00026200 Rroxscaffold_1G00026270 Rroxscaffold_1G00050950 Rroxscaffold_1G00058900 Rroxscaffold_1G00060040 Rroxscaffold_1G00060060 Rroxscaffold_1G00060070 Rroxscaffold_1G00060080 Rroxscaffold_1G00060110 Rroxscaffold_1G00060120 Rroxscaffold_1G00060140 Rroxscaffold_1G00060170 Rroxscaffold_1G00060210 Rroxscaffold_1G00060220 Rroxscaffold_1G00060240 Rroxscaffold_1G00060250 Rroxscaffold_1G00060260 Rroxscaffold_1G00060300 Rroxscaffold_1G00060320 Rroxscaffold_1G00060330 Rroxscaffold_1G00060900 Rroxscaffold_1G00060920 Rroxscaffold_1G00073980 Rroxscaffold_2G00077560 Rroxscaffold_2G00077580 Rroxscaffold_2G00146990 Rroxscaffold_3G00236200 Rroxscaffold_3G00236210 Rroxscaffold_3G00236220 Rroxscaffold_4G00292760 Rroxscaffold_4G00315200 Rroxscaffold_4G00315240 Rroxscaffold_4G00326240 Rroxscaffold_4G00328980 Rroxscaffold_5G00341890 Rroxscaffold_7G00161350 Rroxscaffold_7G00174490 Rroxscaffold_7G00206550
rosa_rugosa Rorug01G0093000 Rorug01G0093100 Rorug01G0128800 Rorug01G0312000 Rorug01G0312000 Rorug02G0581100 Rorug03G0224600 Rorug05G0028500 Rorug05G0032700 Rorug05G0032800 Rorug05G0032900 Rorug05G0032900 Rorug05G0033000 Rorug05G0033100 Rorug05G0033300 Rorug05G0033400 Rorug05G0033900 Rorug05G0034900 Rorug05G0035000 Rorug05G0046900 Rorug05G0047500 Rorug05G0047500 Rorug05G0111600 Rorug05G0287900 Rorug05G0288700 Rorug05G0586900 Rorug06G0231700 Rorug06G0354300 Rorug06G0354400
rosa_samantha Rh1DG122200 Rh2DG686800 Rh5BG136600 Rh6AG344500 Rh6BG351900 Rh6CG359000 Rh6CG359100 Rh6CG387600 Rh6CG480500 Rh6DG344600 Rh6DG467400
rosa_wichuraiana Rw0G010250 Rw0G014820 Rw0G021400 Rw0G023100 Rw1G009450 Rw1G012100 Rw1G012180 Rw1G028360 Rw2G054260 Rw3G024370 Rw3G024420 Rw3G024470 Rw5G010850 Rw5G011010 Rw5G011020 Rw5G011050 Rw5G011080 Rw5G011120 Rw5G012300 Rw5G018470 Rw5G033830 Rw6G008930 Rw6G030070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1675
AatII GACGTC 1 cut(s) 329
Acc36I ACCTGC 1 cut(s) 171
AccB1I GGYRCC 3 cut(s) 686, 840, 1199
AccI GTMKAC 4 cut(s) 1302, 1312, 1476, 1664
AccII CGCG 1 cut(s) 486
AciI CCGC 4 cut(s) 47, 114, 484, 486
AclWI GGATC 2 cut(s) 1016, 2134
AcoI YGGCCR 2 cut(s) 282, 1974
AcsI RAATTY 6 cut(s) 173, 449, 940, 1121, 1218, 1868
AcuI CTGAAG 4 cut(s) 315, 1223, 1587, 1692
AcyI GRCGYC 2 cut(s) 326, 1200
AfaI GTAC 3 cut(s) 55, 554, 2185
AfiI CCNNNNNNNGG 1 cut(s) 43
AflIII ACRYGT 2 cut(s) 411, 2214
AgsI TTSAA 7 cut(s) 586, 652, 674, 912, 1544, 1555, 1779
AjnI CCWGG 3 cut(s) 949, 1755, 2179
AjuI GAANNNNNNNTTGG 2 cut(s) 374, 406
Alw21I GWGCWC 6 cut(s) 754, 938, 1512, 1582, 1740, 2118
Alw26I GTCTC 2 cut(s) 729, 2102
AlwI GGATC 2 cut(s) 1016, 2134
AlwNI CAGNNNCTG 3 cut(s) 74, 77, 1011
Ama87I CYCGRG 2 cut(s) 347, 1739
AoxI GGCC 5 cut(s) 282, 717, 806, 1974, 2018
ApeKI GCWGC 8 cut(s) 71, 74, 77, 352, 618, 1562, 1571, 1637
ApoI RAATTY 6 cut(s) 173, 449, 940, 1121, 1218, 1868
Asp700I GAANNNNTTC 2 cut(s) 696, 1236
AspLEI GCGC 3 cut(s) 518, 1202, 1229
AspS9I GGNCC 4 cut(s) 206, 717, 1759, 2177
AsuHPI GGTGA 4 cut(s) 278, 1724, 1975, 2121
AvaI CYCGRG 2 cut(s) 347, 1739
AvaII GGWCC 3 cut(s) 206, 1759, 2177
BalI TGGCCA 1 cut(s) 284
BanI GGYRCC 3 cut(s) 686, 840, 1199
BanII GRGCYC 1 cut(s) 754
BarI GAAGNNNNNNTAC 6 cut(s) 143, 175, 1452, 1484, 1707, 1739
BbsI GAAGAC 1 cut(s) 2217
Bbv12I GWGCWC 6 cut(s) 754, 938, 1512, 1582, 1740, 2118
BbvI GCAGC 8 cut(s) 61, 64, 83, 339, 630, 1574, 1583, 1649
BccI CCATC 8 cut(s) 217, 740, 872, 879, 1028, 1954, 2159, 2231
BceAI ACGGC 4 cut(s) 383, 445, 1009, 1187
BciT130I CCWGG 3 cut(s) 951, 1757, 2181
BciVI GTATCC 2 cut(s) 974, 1745
BcoDI GTCTC 2 cut(s) 729, 2102
BfaI CTAG 4 cut(s) 51, 1583, 2048, 2199
BfmI CTRYAG 2 cut(s) 616, 2085
BfoI RGCGCY 2 cut(s) 519, 1203
BfuAI ACCTGC 1 cut(s) 171
BfuI GTATCC 2 cut(s) 974, 1745
BlpI GCTNAGC 1 cut(s) 1830
BmcAI AGTACT 1 cut(s) 55
Bme1390I CCNGG 3 cut(s) 951, 1757, 2181
Bme18I GGWCC 3 cut(s) 206, 1759, 2177
BmeT110I CYCGRG 2 cut(s) 347, 1739
BmgT120I GGNCC 4 cut(s) 206, 717, 1759, 2177
BmiI GGNNCC 4 cut(s) 688, 842, 1201, 1761
BmrFI CCNGG 3 cut(s) 951, 1757, 2181
BmrI ACTGGG 2 cut(s) 468, 537
BmsI GCATC 3 cut(s) 1023, 1890, 2080
BmuI ACTGGG 2 cut(s) 468, 537
BpiI GAAGAC 1 cut(s) 2217
BpmI CTGGAG 1 cut(s) 933
Bpu1102I GCTNAGC 1 cut(s) 1830
BpuEI CTTGAG 1 cut(s) 66
BsaAI YACGTR 1 cut(s) 414
BsaBI GATNNNNATC 1 cut(s) 1013
BsaHI GRCGYC 2 cut(s) 326, 1200
BsaJI CCNNGG 9 cut(s) 202, 312, 484, 1203, 1492, 1896, 2040, 2075, 2142
BsaXI ACNNNNNCTCC 4 cut(s) 356, 386, 1188, 1218
Bsc4I CCNNNNNNNGG 1 cut(s) 43
Bse118I RCCGGY 2 cut(s) 804, 837
Bse1I ACTGG 5 cut(s) 463, 543, 666, 1188, 2190
Bse3DI GCAATG 1 cut(s) 630
Bse8I GATNNNNATC 1 cut(s) 1013
BseBI CCWGG 3 cut(s) 951, 1757, 2181
BseDI CCNNGG 9 cut(s) 202, 312, 484, 1203, 1492, 1896, 2040, 2075, 2142
BseGI GGATG 3 cut(s) 537, 871, 2071
BseJI GATNNNNATC 1 cut(s) 1013
BseLI CCNNNNNNNGG 1 cut(s) 43
BseMI GCAATG 1 cut(s) 630
BseMII CTCAG 2 cut(s) 767, 1821
BseNI ACTGG 5 cut(s) 463, 543, 666, 1188, 2190
BseRI GAGGAG 2 cut(s) 936, 1588
BseXI GCAGC 8 cut(s) 61, 64, 83, 339, 630, 1574, 1583, 1649
BseYI CCCAGC 2 cut(s) 91, 518
BsgI GTGCAG 1 cut(s) 1080
Bsh1236I CGCG 1 cut(s) 486
BshFI GGCC 5 cut(s) 284, 719, 808, 1976, 2020
BshNI GGYRCC 3 cut(s) 686, 840, 1199
BsiHKAI GWGCWC 6 cut(s) 754, 938, 1512, 1582, 1740, 2118
BsiHKCI CYCGRG 2 cut(s) 347, 1739
BsiSI CCGG 2 cut(s) 805, 838
BslFI GGGAC 1 cut(s) 1756
BslI CCNNNNNNNGG 1 cut(s) 43
BsmAI GTCTC 2 cut(s) 729, 2102
BsmBI CGTCTC 1 cut(s) 729
BsmFI GGGAC 1 cut(s) 1756
BsmI GAATGC 1 cut(s) 1774
BsnI GGCC 5 cut(s) 284, 719, 808, 1976, 2020
BsoBI CYCGRG 2 cut(s) 347, 1739
Bsp1286I GDGCHC 6 cut(s) 754, 938, 1512, 1582, 1740, 2118
Bsp1407I TGTACA 1 cut(s) 552
Bsp143I GATC 5 cut(s) 369, 1008, 1526, 2089, 2126
Bsp1720I GCTNAGC 1 cut(s) 1830
Bsp19I CCATGG 4 cut(s) 1492, 1896, 2040, 2075
BspACI CCGC 4 cut(s) 47, 114, 484, 486
BspANI GGCC 5 cut(s) 284, 719, 808, 1976, 2020
BspCNI CTCAG 2 cut(s) 766, 1822
BspFNI CGCG 1 cut(s) 486
BspHI TCATGA 1 cut(s) 1504
BspLI GGNNCC 4 cut(s) 688, 842, 1201, 1761
BspMAI CTGCAG 2 cut(s) 620, 2089
BspMI ACCTGC 1 cut(s) 171
BspPI GGATC 2 cut(s) 1016, 2134
BspT107I GGYRCC 3 cut(s) 686, 840, 1199
BsrDI GCAATG 1 cut(s) 630
BsrFI RCCGGY 2 cut(s) 804, 837
BsrGI TGTACA 1 cut(s) 552
BsrI ACTGG 5 cut(s) 463, 543, 666, 1188, 2190
BssAI RCCGGY 2 cut(s) 804, 837
BssECI CCNNGG 9 cut(s) 202, 312, 484, 1203, 1492, 1896, 2040, 2075, 2142
BssMI GATC 5 cut(s) 369, 1008, 1526, 2089, 2126
BssNAI GTATAC 4 cut(s) 1303, 1313, 1477, 1665
BssNI GRCGYC 2 cut(s) 326, 1200
BssT1I CCWWGG 6 cut(s) 202, 1492, 1896, 2040, 2075, 2142
Bst1107I GTATAC 4 cut(s) 1303, 1313, 1477, 1665
Bst2UI CCWGG 3 cut(s) 951, 1757, 2181
Bst4CI ACNGT 4 cut(s) 266, 565, 1732, 1747
Bst6I CTCTTC 3 cut(s) 363, 1506, 2100
BstACI GRCGYC 2 cut(s) 326, 1200
BstAPI GCANNNNNTGC 1 cut(s) 77
BstAUI TGTACA 1 cut(s) 552
BstBAI YACGTR 1 cut(s) 414
BstC8I GCNNGC 6 cut(s) 775, 845, 1063, 1185, 1879, 2204
BstDEI CTNAG 3 cut(s) 753, 1830, 1873
BstDSI CCRYGG 6 cut(s) 484, 1203, 1492, 1896, 2040, 2075
BstF5I GGATG 3 cut(s) 537, 871, 2071
BstFNI CGCG 1 cut(s) 486
BstH2I RGCGCY 2 cut(s) 519, 1203
BstHHI GCGC 3 cut(s) 518, 1202, 1229
BstKTI GATC 5 cut(s) 372, 1011, 1529, 2092, 2129
BstMAI GTCTC 2 cut(s) 729, 2102
BstMBI GATC 5 cut(s) 369, 1008, 1526, 2089, 2126
BstNI CCWGG 3 cut(s) 951, 1757, 2181
BstNSI RCATGY 4 cut(s) 777, 796, 2206, 2218
BstSCI CCNGG 3 cut(s) 949, 1755, 2179
BstSFI CTRYAG 2 cut(s) 616, 2085
BstUI CGCG 1 cut(s) 486
BstV1I GCAGC 8 cut(s) 61, 64, 83, 339, 630, 1574, 1583, 1649
BstV2I GAAGAC 1 cut(s) 2217
BstX2I RGATCY 1 cut(s) 1008
BstXI CCANNNNNNTGG 2 cut(s) 878, 1157
BstYI RGATCY 1 cut(s) 1008
BstZ17I GTATAC 4 cut(s) 1303, 1313, 1477, 1665
BsuI GTATCC 2 cut(s) 974, 1745
BsuRI GGCC 5 cut(s) 284, 719, 808, 1976, 2020
BtgI CCRYGG 6 cut(s) 484, 1203, 1492, 1896, 2040, 2075
BtsCI GGATG 3 cut(s) 537, 871, 2071
BtsI GCAGTG 1 cut(s) 496
BtsIMutI CAGTG 4 cut(s) 496, 550, 561, 2197
BveI ACCTGC 1 cut(s) 171
Cac8I GCNNGC 6 cut(s) 775, 845, 1063, 1185, 1879, 2204
CaiI CAGNNNCTG 3 cut(s) 74, 77, 1011
CciI TCATGA 1 cut(s) 1504
CfoI GCGC 3 cut(s) 518, 1202, 1229
Cfr10I RCCGGY 2 cut(s) 804, 837
Cfr13I GGNCC 4 cut(s) 206, 717, 1759, 2177
Cfr42I CCGCGG 1 cut(s) 487
CseI GACGC 1 cut(s) 746
Csp6I GTAC 3 cut(s) 54, 553, 2184
CspCI CAANNNNNGTGG 2 cut(s) 1830, 1865
CviQI GTAC 3 cut(s) 54, 553, 2184
DdeI CTNAG 3 cut(s) 753, 1830, 1873
DinI GGCGCC 1 cut(s) 1201
DpnI GATC 5 cut(s) 371, 1010, 1528, 2091, 2128
DpnII GATC 5 cut(s) 369, 1008, 1526, 2089, 2126
DrdI GACNNNNNNGTC 1 cut(s) 1675
DseDI GACNNNNNNGTC 1 cut(s) 1675
EaeI YGGCCR 2 cut(s) 282, 1974
Eam1104I CTCTTC 3 cut(s) 363, 1506, 2100
EarI CTCTTC 3 cut(s) 363, 1506, 2100
Ecl136II GAGCTC 1 cut(s) 752
Eco130I CCWWGG 6 cut(s) 202, 1492, 1896, 2040, 2075, 2142
Eco147I AGGCCT 1 cut(s) 2020
Eco24I GRGCYC 1 cut(s) 754
Eco47I GGWCC 3 cut(s) 206, 1759, 2177
Eco53kI GAGCTC 1 cut(s) 752
Eco57I CTGAAG 4 cut(s) 315, 1223, 1587, 1692
Eco88I CYCGRG 2 cut(s) 347, 1739
EcoICRI GAGCTC 1 cut(s) 752
EcoRI GAATTC 3 cut(s) 173, 449, 1868
EcoRII CCWGG 3 cut(s) 949, 1755, 2179
EcoT14I CCWWGG 6 cut(s) 202, 1492, 1896, 2040, 2075, 2142
EcoT38I GRGCYC 1 cut(s) 754
EgeI GGCGCC 1 cut(s) 1201
EheI GGCGCC 1 cut(s) 1201
ErhI CCWWGG 6 cut(s) 202, 1492, 1896, 2040, 2075, 2142
Esp3I CGTCTC 1 cut(s) 729
FalI AAGNNNNNCTT 4 cut(s) 724, 756, 1030, 1062
FaqI GGGAC 1 cut(s) 1756
FauI CCCGC 1 cut(s) 491
FauNDI CATATG 1 cut(s) 2230
FblI GTMKAC 4 cut(s) 1302, 1312, 1476, 1664
FokI GGATG 3 cut(s) 524, 858, 2058
FriOI GRGCYC 1 cut(s) 754
FspBI CTAG 4 cut(s) 51, 1583, 2048, 2199
GlaI GCGC 3 cut(s) 517, 1201, 1228
GsaI CCCAGC 2 cut(s) 95, 522
GsuI CTGGAG 1 cut(s) 933
HaeII RGCGCY 2 cut(s) 519, 1203
HaeIII GGCC 5 cut(s) 284, 719, 808, 1976, 2020
HapII CCGG 2 cut(s) 805, 838
HgaI GACGC 1 cut(s) 746
HhaI GCGC 3 cut(s) 518, 1202, 1229
Hin1I GRCGYC 2 cut(s) 326, 1200
Hin6I GCGC 3 cut(s) 516, 1200, 1227
HinP1I GCGC 3 cut(s) 516, 1200, 1227
HincII GTYRAC 1 cut(s) 1387
HindII GTYRAC 1 cut(s) 1387
HindIII AAGCTT 1 cut(s) 1433
HinfI GANTC 3 cut(s) 12, 377, 818
HpaII CCGG 2 cut(s) 805, 838
HphI GGTGA 4 cut(s) 278, 1724, 1975, 2121
Hpy166II GTNNAC 7 cut(s) 1207, 1303, 1313, 1387, 1477, 1665, 1807
Hpy188I TCNGA 5 cut(s) 376, 756, 1371, 1681, 2126
Hpy188III TCNNGA 8 cut(s) 822, 875, 1241, 1505, 1625, 1695, 1741, 2102
Hpy8I GTNNAC 7 cut(s) 1207, 1303, 1313, 1387, 1477, 1665, 1807
Hpy99I CGWCG 2 cut(s) 995, 1672
HpyAV CCTTC 8 cut(s) 145, 221, 637, 686, 757, 851, 1940, 2045
HpyCH4III ACNGT 4 cut(s) 266, 565, 1732, 1747
HpyCH4IV ACGT 4 cut(s) 288, 326, 413, 1803
HpyF3I CTNAG 3 cut(s) 753, 1830, 1873
HpySE526I ACGT 4 cut(s) 288, 326, 413, 1803
Hsp92I GRCGYC 2 cut(s) 326, 1200
HspAI GCGC 3 cut(s) 516, 1200, 1227
KasI GGCGCC 1 cut(s) 1199
KspI CCGCGG 1 cut(s) 487
Kzo9I GATC 5 cut(s) 369, 1008, 1526, 2089, 2126
LmnI GCTCC 4 cut(s) 749, 1577, 1688, 2044
Lsp1109I GCAGC 8 cut(s) 61, 64, 83, 339, 630, 1574, 1583, 1649
LweI GCATC 3 cut(s) 1023, 1890, 2080
MaeI CTAG 4 cut(s) 51, 1583, 2048, 2199
MaeII ACGT 4 cut(s) 288, 326, 413, 1803
MaeIII GTNAC 7 cut(s) 158, 241, 407, 827, 971, 1170, 1799
MalI GATC 5 cut(s) 371, 1010, 1528, 2091, 2128
MboI GATC 5 cut(s) 369, 1008, 1526, 2089, 2126
MfeI CAATTG 1 cut(s) 184
MflI RGATCY 1 cut(s) 1008
MhlI GDGCHC 6 cut(s) 754, 938, 1512, 1582, 1740, 2118
MlsI TGGCCA 1 cut(s) 284
MluNI TGGCCA 1 cut(s) 284
Mly113I GGCGCC 1 cut(s) 1200
MlyI GAGTC 2 cut(s) 6, 827
Mox20I TGGCCA 1 cut(s) 284
MroXI GAANNNNTTC 2 cut(s) 696, 1236
MscI TGGCCA 1 cut(s) 284
MseI TTAA 5 cut(s) 572, 609, 711, 1838, 1913
MslI CAYNNNNRTG 6 cut(s) 594, 876, 1096, 1422, 1626, 2219
Msp20I TGGCCA 1 cut(s) 284
MspA1I CMGCKG 4 cut(s) 74, 95, 486, 1565
MspI CCGG 2 cut(s) 805, 838
MspR9I CCNGG 3 cut(s) 951, 1757, 2181
MunI CAATTG 1 cut(s) 184
Mva1269I GAATGC 1 cut(s) 1774
MvaI CCWGG 3 cut(s) 951, 1757, 2181
MvnI CGCG 1 cut(s) 486
NarI GGCGCC 1 cut(s) 1200
NcoI CCATGG 4 cut(s) 1492, 1896, 2040, 2075
NdeI CATATG 1 cut(s) 2230
NdeII GATC 5 cut(s) 369, 1008, 1526, 2089, 2126
NlaIV GGNNCC 4 cut(s) 688, 842, 1201, 1761
NmeAIII GCCGAG 2 cut(s) 23, 337
NmuCI GTSAC 3 cut(s) 407, 971, 1799
NspI RCATGY 4 cut(s) 777, 796, 2206, 2218
PaeI GCATGC 2 cut(s) 777, 2206
PaeR7I CTCGAG 1 cut(s) 347
PagI TCATGA 1 cut(s) 1504
PceI AGGCCT 1 cut(s) 2020
PciI ACATGT 1 cut(s) 2214
PctI GAATGC 1 cut(s) 1774
PdmI GAANNNNTTC 2 cut(s) 696, 1236
PfeI GAWTC 1 cut(s) 377
PfoI TCCNGGA 1 cut(s) 949
PleI GAGTC 2 cut(s) 6, 826
PluTI GGCGCC 1 cut(s) 1203
PpsI GAGTC 2 cut(s) 6, 826
Ppu21I YACGTR 1 cut(s) 414
PscI ACATGT 1 cut(s) 2214
Psp124BI GAGCTC 1 cut(s) 754
Psp6I CCWGG 3 cut(s) 949, 1755, 2179
PspFI CCCAGC 2 cut(s) 91, 518
PspGI CCWGG 3 cut(s) 949, 1755, 2179
PspN4I GGNNCC 4 cut(s) 688, 842, 1201, 1761
PspPI GGNCC 4 cut(s) 206, 717, 1759, 2177
PspXI VCTCGAGB 1 cut(s) 347
PstI CTGCAG 2 cut(s) 620, 2089
PstNI CAGNNNCTG 3 cut(s) 74, 77, 1011
PsuI RGATCY 1 cut(s) 1008
PvuII CAGCTG 3 cut(s) 74, 95, 1565
RsaI GTAC 3 cut(s) 55, 554, 2185
RsaNI GTAC 3 cut(s) 54, 553, 2184
RseI CAYNNNNRTG 6 cut(s) 594, 876, 1096, 1422, 1626, 2219
SacI GAGCTC 1 cut(s) 754
SacII CCGCGG 1 cut(s) 487
SaqAI TTAA 5 cut(s) 572, 609, 711, 1838, 1913
Sau3AI GATC 5 cut(s) 369, 1008, 1526, 2089, 2126
Sau96I GGNCC 4 cut(s) 206, 717, 1759, 2177
ScaI AGTACT 1 cut(s) 55
SchI GAGTC 2 cut(s) 6, 827
ScrFI CCNGG 3 cut(s) 951, 1757, 2181
SduI GDGCHC 6 cut(s) 754, 938, 1512, 1582, 1740, 2118
SfaNI GCATC 3 cut(s) 1023, 1890, 2080
SfcI CTRYAG 2 cut(s) 616, 2085
SfoI GGCGCC 1 cut(s) 1201
Sfr274I CTCGAG 1 cut(s) 347
Sfr303I CCGCGG 1 cut(s) 487
SgrBI CCGCGG 1 cut(s) 487
SinI GGWCC 3 cut(s) 206, 1759, 2177
SlaI CTCGAG 1 cut(s) 347
SmiMI CAYNNNNRTG 6 cut(s) 594, 876, 1096, 1422, 1626, 2219
SmlI CTYRAG 2 cut(s) 81, 347
SmoI CTYRAG 2 cut(s) 81, 347
SphI GCATGC 2 cut(s) 777, 2206
SseBI AGGCCT 1 cut(s) 2020
SsiI CCGC 4 cut(s) 47, 114, 484, 486
SspDI GGCGCC 1 cut(s) 1199
SspI AATATT 2 cut(s) 279, 1645
SspMI CTAG 4 cut(s) 51, 1583, 2048, 2199
SstI GAGCTC 1 cut(s) 754
StuI AGGCCT 1 cut(s) 2020
StyD4I CCNGG 3 cut(s) 949, 1755, 2179
StyI CCWWGG 6 cut(s) 202, 1492, 1896, 2040, 2075, 2142
TaaI ACNGT 4 cut(s) 266, 565, 1732, 1747
TaiI ACGT 4 cut(s) 291, 329, 416, 1806
TaqI TCGA 2 cut(s) 348, 1624
TatI WGTACW 2 cut(s) 53, 552
TauI GCSGC 3 cut(s) 50, 117, 489
TfiI GAWTC 1 cut(s) 377
Tru1I TTAA 5 cut(s) 572, 609, 711, 1838, 1913
Tru9I TTAA 5 cut(s) 572, 609, 711, 1838, 1913
TscAI CASTG 4 cut(s) 496, 550, 568, 2197
TseFI GTSAC 3 cut(s) 407, 971, 1799
TseI GCWGC 8 cut(s) 71, 74, 77, 352, 618, 1562, 1571, 1637
Tsp45I GTSAC 3 cut(s) 407, 971, 1799
TspGWI ACGGA 1 cut(s) 326
TspRI CASTG 4 cut(s) 496, 550, 568, 2197
VpaK11BI GGWCC 3 cut(s) 206, 1759, 2177
XapI RAATTY 6 cut(s) 173, 449, 940, 1121, 1218, 1868
XceI RCATGY 4 cut(s) 777, 796, 2206, 2218
XhoI CTCGAG 1 cut(s) 347
XmiI GTMKAC 4 cut(s) 1302, 1312, 1476, 1664
XmnI GAANNNNTTC 2 cut(s) 696, 1236
XspI CTAG 4 cut(s) 51, 1583, 2048, 2199
ZraI GACGTC 1 cut(s) 327
ZrmI AGTACT 1 cut(s) 55
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.