FvH4_3g10340

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000066
Physical Location & Seq
Reverse (-)
6045848 .. 6049051
3204 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g10340.t1

Sequence Viewer

Length: 2022 bp
ATGCAAATATCGAAGCGAATAGGGCGAGATTGTTACGATGAAGAGGGCAACGAAACTAGCACAGCAGGAACGTTCAGTTTGGGACCTCCAAACACCATATCTGATACCCAAAACAAGTTCTACGCCGTTGGTTGCGACACTTATGCAACCATGGAAGGCTTCCGAGGGGAAGAAAAAGTCATTACTGGGTGCATTTCAGTATGCAACAGCCTCAGCAGTGTTAATAAGAACTCTTGCTCTGGCATTGGGTGTTGCCAGATTAACATCCCCAGTGGACTGACCAATCTCACTTTCGAGTTGAGAAGCTTCTACAATCATAAGAATGTATCGGAGTTTAACCCCTGCAGCTACGCCTTCATTGCAGAGCAAGGCCGGTTCAATTTCAGCTCTTCTAGTTTTGAACAACTGAATGGCATTGAACGGCTTCCAATGGTTCTTAATTGGGCCATTGTGAATGACGCAGATCCCTGCGATGAGACTCAGAACAGGAAGGGTTCCGCATGCAAGGCTAATAGCAAGTGCTTCAACCCAGCTATCAATGAGTCTGTAGGTTACTTGTGCCAGTGCTTGCCTGGTTATGAAGGAAACCCGTACCACCCAGATGGTTGCACAGATATTGATGAGTGCAAAGACCCAAACCTCTGCCAGAAAGGACAATGCTTGAATCTTCTACCTCCACAAAATTACACTTGTTCATCATGTCACAAAGGATACAAACACGACGGCACCAATGACAAGAGTTGCATCAAAGACGATCTCGAAAGAAGCTCAAAGATCGTTCTCCTGCTTATTGTTTCATTGGGTGTAAGTATGGGCTTGTTGGTTTTTTTCCTTGGGATTTCGTGGATATGTTGGGGAATGAAGAAAAGAGAGTTCATTAAGCAGAAGGAAAAGTACTTCAAAGAGAATGGTGGCTTATTGTTACTGCAACAACTCGCCAGTGATAATGGAAGCTCGATGAAGACAACAAAAATCTTTACCGCTGATGAACTTGAGAGGGCAACAAACAACTACCATGAGAGTAGAATCCTTGGAGAAGGAGGTAATGGAACAGTTTACAAAGGAATACTACCGGATGACACAGTGGTTGCCATAAAGAAGTCGAAAGGTACTAGTGCCATGACCCAGAGTGATCAATTTGTTAACGAAGTGATTATCCTTTCTCAAATTAACCACATAAATGTGGTGAAGCTATTAGGTTGCTGTTTCGAAACAGAAGTTCCTTTACTAGTATACGAATTCATTACCCATGGCACTCTTTATGAGCACATTCATAAAAGAAGATCACCTCTCCCTTTTGAATTACGAATGAAGATAGCAACTCAAAGCGCAGAAGCATTGTCTCACTTACACTCGTCAATTTCCACACCAATCATACATCGAGATGTGAAAACGGCAAATATTCTGTTAGCTAATGATTACACGGCGAAAGTGTCAGATTTCGGAGCTTCCCGTTTGGTTCATTCAGGAACTGATATGCAAACTTTGGTGCTCGGGACATTTGGATACCTAGACCCTGAATATCTGCAATCAAACCAGCTAACAGAAAAGAGTGACGTTTACAGCTTTGGAGTTGTCCTAGTGGAACTAATAACAAGCAAAGTTCCACTTTCTAAAGATAGATGCTTAGCAAGCATCTTTGTGGTTTCCATGGAAGAAGATTGGTTGGATCAAATTCTTGACGATGATATAGTGAACGATGGAAACATTGAGACGGTAAAGAAAGTGGCCCATCTCGCAAAGCGATGCTTGATGGTAAAAGGGGAGGAAAGGCCTACCATGAAAGAAGTTGCGAAAGAGCTCGAGGAAATAATGAGTGTCACAGCAAAACATCCGTGGGGATTTAATGCTAGTTTCTACCGAGAAGAGACTGAGTACTTGCTCGGGTCACTCAATTCAGACGGTTACGTCGTTGGTGTTGGGGATGATTGCAGTTCTAGTGGTCTAACCAGTGGTACGGCCAATGCATATGACAGCATGCAAAAACAACTGCAGTTGATGCCATATGGCGGTGGACGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK

Protein Analysis

674

Amino Acids

74.45

Weight (kDa)

5.4

Isoelectric Point (pI)

39.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 338 - 599 5.1e-49 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 341 - 601 4.1e-49 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21210 AT1G21230 AT1G21240 AT1G21240 AT1G21245 AT1G21250 AT1G21270 AT1G22720 AT3G25490
fragaria_vesca FvH4_2g35051 FvH4_2g35051 FvH4_2g35060 FvH4_3g10281 FvH4_3g10285 FvH4_3g10286 FvH4_3g10311 FvH4_3g10311 FvH4_3g10320 FvH4_3g10332 FvH4_3g10340 FvH4_3g10350 FvH4_3g10360 FvH4_3g10390 FvH4_3g10412 FvH4_3g10413 FvH4_3g10461 FvH4_3g10462 FvH4_3g10470 FvH4_3g10510 FvH4_3g10520 FvH4_3g10530 FvH4_3g10550 FvH4_3g10560 FvH4_3g10611 FvH4_3g11730 FvH4_6g53140 FvH4_6g53142 FvH4_7g31880 FvH4_7g31910 FvH4_7g31920
malus_domestica MD05G1269900.v1.1 MD05G1270000.v1.1 MD05G1270200.v1.1 MD05G1270400.v1.1 MD05G1270500.v1.1 MD05G1270600.v1.1 MD05G1270800.v1.1 MD05G1270900.v1.1 MD07G1183800.v1.1 MD08G1107600.v1.1 MD10G1248500.v1.1 MD10G1249200.v1.1 MD10G1249500.v1.1 MD10G1250000.v1.1 MD10G1250500.v1.1 MD10G1250900.v1.1 MD10G1251200.v1.1 MD10G1251400.v1.1 MD13G1004600.v1.1 MD15G1088400.v1.1
prunus_persica Prupe.1G442400_v2.0.a1 Prupe.1G442500_v2.0.a1 Prupe.2G223400_v2.0.a1 Prupe.4G091900_v2.0.a1 Prupe.4G093200_v2.0.a1 Prupe.4G093300_v2.0.a1 Prupe.4G093400_v2.0.a1 Prupe.4G093500_v2.0.a1 Prupe.4G094100_v2.0.a1 Prupe.4G094200_v2.0.a1 Prupe.4G094300_v2.0.a1 Prupe.6G358300_v2.0.a1
pyrus_communis pycom08g08990 pycom10g20660 pycom10g20670 pycom10g20680 pycom10g20710 pycom10g20740 pycom13g00400 pycom15g08290
rosa_chinensis RchiOBHm_Chr1g0326861 RchiOBHm_Chr1g0326871 RchiOBHm_Chr1g0331391 RchiOBHm_Chr1g0336911 RchiOBHm_Chr1g0364001 RchiOBHm_Chr2g0174541 RchiOBHm_Chr2g0175041 RchiOBHm_Chr2g0175101 RchiOBHm_Chr2g0175121 RchiOBHm_Chr3g0486331 RchiOBHm_Chr5g0015581 RchiOBHm_Chr5g0016551 RchiOBHm_Chr5g0016561 RchiOBHm_Chr5g0016581 RchiOBHm_Chr5g0016601 RchiOBHm_Chr5g0016611 RchiOBHm_Chr5g0016641 RchiOBHm_Chr5g0016661 RchiOBHm_Chr5g0016691 RchiOBHm_Chr5g0016701 RchiOBHm_Chr5g0016731 RchiOBHm_Chr5g0016741 RchiOBHm_Chr5g0016811 RchiOBHm_Chr5g0016841 RchiOBHm_Chr5g0016871 RchiOBHm_Chr5g0016881 RchiOBHm_Chr5g0016931 RchiOBHm_Chr5g0017021 RchiOBHm_Chr5g0017041 RchiOBHm_Chr5g0017071 RchiOBHm_Chr5g0017091 RchiOBHm_Chr5g0017121 RchiOBHm_Chr5g0017141 RchiOBHm_Chr5g0017291 RchiOBHm_Chr5g0017321 RchiOBHm_Chr5g0017401 RchiOBHm_Chr5g0019001 RchiOBHm_Chr5g0019071 RchiOBHm_Chr5g0028941 RchiOBHm_Chr5g0052141 RchiOBHm_Chr5g0054721 RchiOBHm_Chr5g0054801 RchiOBHm_Chr5g0054931 RchiOBHm_Chr5g0054941 RchiOBHm_Chr6g0259291 RchiOBHm_Chr6g0292991 RchiOBHm_Chr6g0306911
rosa_laevigata RLG00000009465 RLG00000009468 RLG00000010755 RLG00000011976 RLG00000014541 RLG00000014542 RLG00000015169 RLG00000022308 RLG00000027529 RLG00000029324 RLG00000030244 RLG00000030245 RLG00000032210 RLG00000032211 RLG00000032257 RLG00000032259 RLG00000032260 RLG00000032261 RLG00000032262 RLG00000032268 RLG00000032269 RLG00000032272 RLG00000032282 RLG00000032283 RLG00000032308 RLG00000032413 RLG00000033129 RLG00000034945
rosa_multiflora Rmu_co8241929.1_g000001 Rmu_co8269227.1_g000001 Rmu_co8461259.1_g000001 Rmu_co8495385.1_g000001 Rmu_sc0000028.1_g000003 Rmu_sc0000083.1_g000009 Rmu_sc0000083.1_g000010 Rmu_sc0000083.1_g000031 Rmu_sc0000083.1_g000043 Rmu_sc0000083.1_g000046 Rmu_sc0000083.1_g000047 Rmu_sc0000563.1_g000024 Rmu_sc0000842.1_g000012 Rmu_sc0001235.1_g000001 Rmu_sc0001235.1_g000005 Rmu_sc0001235.1_g000009 Rmu_sc0001235.1_g000015 Rmu_sc0001235.1_g000016 Rmu_sc0001235.1_g000017 Rmu_sc0001473.1_g000036 Rmu_sc0001607.1_g000016 Rmu_sc0001788.1_g000006 Rmu_sc0002018.1_g000008 Rmu_sc0002028.1_g000001 Rmu_sc0002680.1_g000011 Rmu_sc0002798.1_g000023 Rmu_sc0002886.1_g000023 Rmu_sc0002989.1_g000012 Rmu_sc0003064.1_g000009 Rmu_sc0003382.1_g000006 Rmu_sc0003630.1_g000002 Rmu_sc0003630.1_g000047 Rmu_sc0003630.1_g000066 Rmu_sc0003630.1_g000067 Rmu_sc0003649.1_g000010 Rmu_sc0003688.1_g000005 Rmu_sc0003920.1_g000007 Rmu_sc0004765.1_g000007 Rmu_sc0004765.1_g000008 Rmu_sc0004920.1_g000011 Rmu_sc0005223.1_g000008 Rmu_sc0005223.1_g000010 Rmu_sc0005223.1_g000015 Rmu_sc0005223.1_g000016 Rmu_sc0005319.1_g000014 Rmu_sc0005319.1_g000015 Rmu_sc0005319.1_g000018 Rmu_sc0005319.1_g000019 Rmu_sc0005319.1_g000025 Rmu_sc0005418.1_g000007 Rmu_sc0005418.1_g000012 Rmu_sc0006106.1_g000011 Rmu_sc0006914.1_g000012 Rmu_sc0007023.1_g000001 Rmu_sc0007662.1_g000001 Rmu_sc0007920.1_g000002 Rmu_sc0007920.1_g000013 Rmu_sc0008053.1_g000016 Rmu_sc0008053.1_g000017 Rmu_sc0008053.1_g000018 Rmu_sc0008053.1_g000023 Rmu_sc0008053.1_g000050 Rmu_sc0009042.1_g000007 Rmu_sc0009042.1_g000008 Rmu_sc0010642.1_g000008 Rmu_sc0011109.1_g000002 Rmu_sc0011314.1_g000006 Rmu_sc0011974.1_g000006 Rmu_sc0012179.1_g000009 Rmu_sc0015169.1_g000001 Rmu_sc0016368.1_g000002 Rmu_sc0022659.1_g000001 Rmu_sc0027371.1_g000001 Rmu_sc0028213.1_g000001 Rmu_sc0034526.1_g000001 Rmu_sc0039366.1_g000002 Rmu_ssc0000050.1_g000084 Rmu_ssc0000050.1_g000088 Rmu_ssc0000167.1_g000017
rosa_roxburghii Rroxscaffold_1G00011340 Rroxscaffold_1G00025710 Rroxscaffold_1G00025870 Rroxscaffold_1G00026070 Rroxscaffold_1G00026170 Rroxscaffold_1G00026200 Rroxscaffold_1G00026270 Rroxscaffold_1G00050950 Rroxscaffold_1G00058900 Rroxscaffold_1G00060040 Rroxscaffold_1G00060060 Rroxscaffold_1G00060070 Rroxscaffold_1G00060080 Rroxscaffold_1G00060110 Rroxscaffold_1G00060120 Rroxscaffold_1G00060140 Rroxscaffold_1G00060170 Rroxscaffold_1G00060210 Rroxscaffold_1G00060220 Rroxscaffold_1G00060240 Rroxscaffold_1G00060250 Rroxscaffold_1G00060260 Rroxscaffold_1G00060300 Rroxscaffold_1G00060320 Rroxscaffold_1G00060330 Rroxscaffold_1G00060900 Rroxscaffold_1G00060920 Rroxscaffold_1G00073980 Rroxscaffold_2G00077560 Rroxscaffold_2G00077580 Rroxscaffold_2G00146990 Rroxscaffold_3G00236200 Rroxscaffold_3G00236210 Rroxscaffold_3G00236220 Rroxscaffold_4G00292760 Rroxscaffold_4G00315200 Rroxscaffold_4G00315240 Rroxscaffold_4G00326240 Rroxscaffold_4G00328980 Rroxscaffold_5G00341890 Rroxscaffold_7G00161350 Rroxscaffold_7G00174490 Rroxscaffold_7G00206550
rosa_rugosa Rorug01G0093000 Rorug01G0093100 Rorug01G0128800 Rorug01G0312000 Rorug01G0312000 Rorug02G0581100 Rorug03G0224600 Rorug05G0028500 Rorug05G0032700 Rorug05G0032800 Rorug05G0032900 Rorug05G0032900 Rorug05G0033000 Rorug05G0033100 Rorug05G0033300 Rorug05G0033400 Rorug05G0033900 Rorug05G0034900 Rorug05G0035000 Rorug05G0046900 Rorug05G0047500 Rorug05G0047500 Rorug05G0111600 Rorug05G0287900 Rorug05G0288700 Rorug05G0586900 Rorug06G0231700 Rorug06G0354300 Rorug06G0354400
rosa_samantha Rh1DG122200 Rh2DG686800 Rh5BG136600 Rh6AG344500 Rh6BG351900 Rh6CG359000 Rh6CG359100 Rh6CG387600 Rh6CG480500 Rh6DG344600 Rh6DG467400
rosa_wichuraiana Rw0G010250 Rw0G014820 Rw0G021400 Rw0G023100 Rw1G009450 Rw1G012100 Rw1G012180 Rw1G028360 Rw2G054260 Rw3G024370 Rw3G024420 Rw3G024470 Rw5G010850 Rw5G011010 Rw5G011020 Rw5G011050 Rw5G011080 Rw5G011120 Rw5G012300 Rw5G018470 Rw5G033830 Rw6G008930 Rw6G030070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1907
AccB1I GGYRCC 1 cut(s) 725
AccI GTMKAC 1 cut(s) 1233
AciI CCGC 3 cut(s) 498, 981, 2010
AclI AACGTT 1 cut(s) 71
AclWI GGATC 2 cut(s) 458, 1677
AcoI YGGCCR 1 cut(s) 1959
AcsI RAATTY 2 cut(s) 1238, 1674
AfaI GTAC 5 cut(s) 593, 896, 1111, 1877, 1957
AfiI CCNNNNNNNGG 1 cut(s) 2009
AgsI TTSAA 7 cut(s) 379, 401, 419, 526, 664, 901, 1301
AhlI ACTAGT 2 cut(s) 1112, 1228
AjnI CCWGG 1 cut(s) 571
AjuI GAANNNNNNNTTGG 2 cut(s) 275, 307
Alw21I GWGCWC 3 cut(s) 1269, 1494, 1803
Alw26I GTCTC 4 cut(s) 470, 1347, 1706, 1862
AlwI GGATC 2 cut(s) 458, 1677
AlwNI CAGNNNCTG 1 cut(s) 1472
Ama87I CYCGRG 3 cut(s) 1493, 1802, 1883
AoxI GGCC 5 cut(s) 370, 444, 1728, 1772, 1959
ApeKI GCWGC 1 cut(s) 345
ApoI RAATTY 2 cut(s) 1238, 1674
Asp700I GAANNNNTTC 1 cut(s) 423
AspLEI GCGC 1 cut(s) 1331
AspS9I GGNCC 3 cut(s) 83, 444, 1729
AsuHPI GGTGA 2 cut(s) 1198, 1278
AsuII TTCGAA 1 cut(s) 1209
AvaI CYCGRG 3 cut(s) 1493, 1802, 1883
AvaII GGWCC 1 cut(s) 83
BanI GGYRCC 1 cut(s) 725
BanII GRGCYC 1 cut(s) 1803
BarI GAAGNNNNNNTAC 4 cut(s) 878, 910, 1209, 1241
BbsI GAAGAC 1 cut(s) 968
Bbv12I GWGCWC 3 cut(s) 1269, 1494, 1803
BbvCI CCTCAGC 1 cut(s) 212
BbvI GCAGC 1 cut(s) 357
BccI CCATC 4 cut(s) 596, 1694, 1740, 1747
BceAI ACGGC 6 cut(s) 110, 437, 739, 1410, 1440, 1974
BcgI CGANNNNNNTGC 2 cut(s) 125, 159
BciT130I CCWGG 1 cut(s) 573
BciVI GTATCC 2 cut(s) 704, 1499
BclI TGATCA 1 cut(s) 1132
BcoDI GTCTC 4 cut(s) 470, 1347, 1706, 1862
BcuI ACTAGT 2 cut(s) 1112, 1228
BfaI CTAG 8 cut(s) 57, 393, 1113, 1229, 1511, 1580, 1851, 1938
BfmI CTRYAG 3 cut(s) 343, 546, 1991
BfuI GTATCC 2 cut(s) 704, 1499
BisI GCNGC 1 cut(s) 346
BlpI GCTNAGC 1 cut(s) 1627
BlsI GCNGC 1 cut(s) 347
BmcAI AGTACT 2 cut(s) 896, 1877
Bme1390I CCNGG 1 cut(s) 573
Bme18I GGWCC 1 cut(s) 83
BmeT110I CYCGRG 3 cut(s) 1493, 1802, 1883
BmgT120I GGNCC 3 cut(s) 83, 444, 1729
BmiI GGNNCC 3 cut(s) 84, 496, 727
BmrFI CCNGG 1 cut(s) 573
BmrI ACTGGG 2 cut(s) 195, 264
BmsI GCATC 5 cut(s) 753, 1613, 1644, 1736, 1989
BmuI ACTGGG 2 cut(s) 195, 264
BpiI GAAGAC 1 cut(s) 968
Bpu10I CCTNAGC 1 cut(s) 212
Bpu1102I GCTNAGC 1 cut(s) 1627
Bpu14I TTCGAA 1 cut(s) 1209
BpuEI CTTGAG 1 cut(s) 1013
BsaBI GATNNNNATC 1 cut(s) 263
BsaJI CCNNGG 7 cut(s) 150, 163, 832, 1030, 1249, 1650, 1835
BsaWI WCCGGW 1 cut(s) 1072
Bsc4I CCNNNNNNNGG 1 cut(s) 2009
Bse118I RCCGGY 1 cut(s) 372
Bse1I ACTGG 5 cut(s) 190, 270, 562, 939, 1950
Bse3DI GCAATG 1 cut(s) 357
Bse8I GATNNNNATC 1 cut(s) 263
BseBI CCWGG 1 cut(s) 573
BseDI CCNNGG 7 cut(s) 150, 163, 832, 1030, 1249, 1650, 1835
BseGI GGATG 4 cut(s) 264, 1081, 1831, 1930
BseJI GATNNNNATC 1 cut(s) 263
BseLI CCNNNNNNNGG 1 cut(s) 2009
BseMI GCAATG 1 cut(s) 357
BseMII CTCAG 3 cut(s) 226, 494, 1863
BseNI ACTGG 5 cut(s) 190, 270, 562, 939, 1950
BseXI GCAGC 1 cut(s) 357
BseYI CCCAGC 1 cut(s) 529
BshFI GGCC 5 cut(s) 372, 446, 1730, 1774, 1961
BshNI GGYRCC 1 cut(s) 725
BsiHKAI GWGCWC 3 cut(s) 1269, 1494, 1803
BsiHKCI CYCGRG 3 cut(s) 1493, 1802, 1883
BsiSI CCGG 2 cut(s) 373, 1073
BslFI GGGAC 2 cut(s) 96, 1510
BslI CCNNNNNNNGG 1 cut(s) 2009
BsmAI GTCTC 4 cut(s) 470, 1347, 1706, 1862
BsmBI CGTCTC 1 cut(s) 1706
BsmFI GGGAC 2 cut(s) 96, 1510
BsnI GGCC 5 cut(s) 372, 446, 1730, 1774, 1961
BsoBI CYCGRG 3 cut(s) 1493, 1802, 1883
Bsp119I TTCGAA 1 cut(s) 1209
Bsp1286I GDGCHC 3 cut(s) 1269, 1494, 1803
Bsp143I GATC 6 cut(s) 463, 754, 774, 1132, 1283, 1669
Bsp1720I GCTNAGC 1 cut(s) 1627
Bsp19I CCATGG 3 cut(s) 150, 1249, 1650
BspACI CCGC 3 cut(s) 498, 981, 2010
BspANI GGCC 5 cut(s) 372, 446, 1730, 1774, 1961
BspCNI CTCAG 3 cut(s) 225, 493, 1864
BspLI GGNNCC 3 cut(s) 84, 496, 727
BspMAI CTGCAG 2 cut(s) 347, 1995
BspPI GGATC 2 cut(s) 458, 1677
BspQI GCTCTTC 1 cut(s) 394
BspT104I TTCGAA 1 cut(s) 1209
BspT107I GGYRCC 1 cut(s) 725
BsrDI GCAATG 1 cut(s) 357
BsrFI RCCGGY 1 cut(s) 372
BsrI ACTGG 5 cut(s) 190, 270, 562, 939, 1950
BssAI RCCGGY 1 cut(s) 372
BssECI CCNNGG 7 cut(s) 150, 163, 832, 1030, 1249, 1650, 1835
BssMI GATC 6 cut(s) 463, 754, 774, 1132, 1283, 1669
BssNAI GTATAC 1 cut(s) 1234
BssT1I CCWWGG 5 cut(s) 150, 832, 1030, 1249, 1650
Bst1107I GTATAC 1 cut(s) 1234
Bst2UI CCWGG 1 cut(s) 573
Bst4CI ACNGT 4 cut(s) 1054, 1084, 1717, 1904
Bst6I CTCTTC 3 cut(s) 36, 394, 1860
BstAPI GCANNNNNTGC 1 cut(s) 1999
BstBI TTCGAA 1 cut(s) 1209
BstC8I GCNNGC 4 cut(s) 502, 569, 1633, 1979
BstDEI CTNAG 4 cut(s) 212, 480, 1627, 1872
BstDSI CCRYGG 4 cut(s) 150, 1249, 1650, 1835
BstF5I GGATG 4 cut(s) 264, 1081, 1831, 1930
BstHHI GCGC 1 cut(s) 1331
BstKTI GATC 6 cut(s) 466, 757, 777, 1135, 1286, 1672
BstMAI GTCTC 4 cut(s) 470, 1347, 1706, 1862
BstMBI GATC 6 cut(s) 463, 754, 774, 1132, 1283, 1669
BstMWI GCNNNNNNNGC 6 cut(s) 22, 359, 506, 1632, 1736, 1999
BstNI CCWGG 1 cut(s) 573
BstNSI RCATGY 2 cut(s) 504, 1981
BstSCI CCNGG 1 cut(s) 571
BstSFI CTRYAG 3 cut(s) 343, 546, 1991
BstV1I GCAGC 1 cut(s) 357
BstV2I GAAGAC 1 cut(s) 968
BstX2I RGATCY 1 cut(s) 463
BstXI CCANNNNNNTGG 1 cut(s) 602
BstYI RGATCY 1 cut(s) 463
BstZ17I GTATAC 1 cut(s) 1234
BsuI GTATCC 2 cut(s) 704, 1499
BsuRI GGCC 5 cut(s) 372, 446, 1730, 1774, 1961
BtgI CCRYGG 4 cut(s) 150, 1249, 1650, 1835
BtgZI GCGATG 2 cut(s) 486, 1759
BtsCI GGATG 4 cut(s) 264, 1081, 1831, 1930
BtsI GCAGTG 1 cut(s) 223
BtsIMutI CAGTG 6 cut(s) 223, 277, 569, 946, 1089, 1957
Cac8I GCNNGC 4 cut(s) 502, 569, 1633, 1979
CaiI CAGNNNCTG 1 cut(s) 1472
CfoI GCGC 1 cut(s) 1331
Cfr10I RCCGGY 1 cut(s) 372
Cfr13I GGNCC 3 cut(s) 83, 444, 1729
CseI GACGC 1 cut(s) 467
Csp6I GTAC 5 cut(s) 592, 895, 1110, 1876, 1956
CviAII CATG 9 cut(s) 151, 501, 699, 1016, 1120, 1250, 1651, 1780, 1978
CviQI GTAC 5 cut(s) 592, 895, 1110, 1876, 1956
DdeI CTNAG 4 cut(s) 212, 480, 1627, 1872
DpnI GATC 6 cut(s) 465, 756, 776, 1134, 1285, 1671
DpnII GATC 6 cut(s) 463, 754, 774, 1132, 1283, 1669
DrdI GACNNNNNNGTC 1 cut(s) 1907
DseDI GACNNNNNNGTC 1 cut(s) 1907
EaeI YGGCCR 1 cut(s) 1959
Eam1104I CTCTTC 3 cut(s) 36, 394, 1860
EarI CTCTTC 3 cut(s) 36, 394, 1860
Ecl136II GAGCTC 1 cut(s) 1801
Eco130I CCWWGG 5 cut(s) 150, 832, 1030, 1249, 1650
Eco147I AGGCCT 1 cut(s) 1774
Eco24I GRGCYC 1 cut(s) 1803
Eco47I GGWCC 1 cut(s) 83
Eco53kI GAGCTC 1 cut(s) 1801
Eco88I CYCGRG 3 cut(s) 1493, 1802, 1883
EcoICRI GAGCTC 1 cut(s) 1801
EcoO109I RGGNCCY 1 cut(s) 83
EcoRI GAATTC 1 cut(s) 1238
EcoRII CCWGG 1 cut(s) 571
EcoT14I CCWWGG 5 cut(s) 150, 832, 1030, 1249, 1650
EcoT22I ATGCAT 1 cut(s) 1969
EcoT38I GRGCYC 1 cut(s) 1803
ErhI CCWWGG 5 cut(s) 150, 832, 1030, 1249, 1650
Esp3I CGTCTC 1 cut(s) 1706
FaeI CATG 9 cut(s) 154, 504, 702, 1019, 1123, 1253, 1654, 1783, 1981
FalI AAGNNNNNCTT 4 cut(s) 1593, 1625, 1733, 1765
FaqI GGGAC 2 cut(s) 96, 1510
FatI CATG 9 cut(s) 150, 500, 698, 1015, 1119, 1249, 1650, 1779, 1977
FauNDI CATATG 2 cut(s) 1969, 2005
FbaI TGATCA 1 cut(s) 1132
FblI GTMKAC 1 cut(s) 1233
Fnu4HI GCNGC 1 cut(s) 346
FokI GGATG 4 cut(s) 251, 1088, 1818, 1937
FriOI GRGCYC 1 cut(s) 1803
Fsp4HI GCNGC 1 cut(s) 346
FspBI CTAG 8 cut(s) 57, 393, 1113, 1229, 1511, 1580, 1851, 1938
GlaI GCGC 1 cut(s) 1330
GluI GCNGC 1 cut(s) 346
GsaI CCCAGC 1 cut(s) 533
HaeIII GGCC 5 cut(s) 372, 446, 1730, 1774, 1961
HapII CCGG 2 cut(s) 373, 1073
HgaI GACGC 1 cut(s) 467
HhaI GCGC 1 cut(s) 1331
Hin1II CATG 9 cut(s) 154, 504, 702, 1019, 1123, 1253, 1654, 1783, 1981
Hin6I GCGC 1 cut(s) 1329
HinP1I GCGC 1 cut(s) 1329
HincII GTYRAC 1 cut(s) 1144
HindII GTYRAC 1 cut(s) 1144
HindIII AAGCTT 1 cut(s) 304
HinfI GANTC 4 cut(s) 478, 542, 664, 1026
HpaI GTTAAC 1 cut(s) 1144
HpaII CCGG 2 cut(s) 373, 1073
HphI GGTGA 2 cut(s) 1198, 1278
Hpy166II GTNNAC 7 cut(s) 275, 1057, 1144, 1234, 1561, 1696, 2015
Hpy188I TCNGA 7 cut(s) 103, 164, 331, 483, 1438, 1445, 1900
Hpy188III TCNNGA 5 cut(s) 758, 1382, 1467, 1495, 1679
Hpy8I GTNNAC 7 cut(s) 275, 1057, 1144, 1234, 1561, 1696, 2015
Hpy99I CGWCG 2 cut(s) 725, 1913
HpyAV CCTTC 6 cut(s) 149, 364, 484, 575, 880, 1031
HpyCH4III ACNGT 4 cut(s) 1054, 1084, 1717, 1904
HpyCH4IV ACGT 4 cut(s) 71, 1557, 1908, 2017
HpyF10VI GCNNNNNNNGC 6 cut(s) 22, 359, 506, 1632, 1736, 1999
HpyF3I CTNAG 4 cut(s) 212, 480, 1627, 1872
HpySE526I ACGT 4 cut(s) 71, 1557, 1908, 2017
Hsp92II CATG 9 cut(s) 154, 504, 702, 1019, 1123, 1253, 1654, 1783, 1981
HspAI GCGC 1 cut(s) 1329
Ksp22I TGATCA 1 cut(s) 1132
KspAI GTTAAC 1 cut(s) 1144
Kzo9I GATC 6 cut(s) 463, 754, 774, 1132, 1283, 1669
LguI GCTCTTC 1 cut(s) 394
LmnI GCTCC 1 cut(s) 1445
Lsp1109I GCAGC 1 cut(s) 357
LweI GCATC 5 cut(s) 753, 1613, 1644, 1736, 1989
MaeI CTAG 8 cut(s) 57, 393, 1113, 1229, 1511, 1580, 1851, 1938
MaeII ACGT 4 cut(s) 71, 1557, 1908, 2017
MaeIII GTNAC 8 cut(s) 32, 551, 701, 921, 1553, 1819, 1887, 1904
MalI GATC 6 cut(s) 465, 756, 776, 1134, 1285, 1671
MboI GATC 6 cut(s) 463, 754, 774, 1132, 1283, 1669
MflI RGATCY 1 cut(s) 463
MhlI GDGCHC 3 cut(s) 1269, 1494, 1803
MlyI GAGTC 2 cut(s) 472, 551
MmeI TCCRAC 1 cut(s) 1647
Mph1103I ATGCAT 1 cut(s) 1969
MroXI GAANNNNTTC 1 cut(s) 423
MseI TTAA 8 cut(s) 222, 261, 336, 438, 879, 1143, 1170, 1845
MslI CAYNNNNRTG 6 cut(s) 321, 600, 1179, 1181, 1383, 1640
MspA1I CMGCKG 1 cut(s) 983
MspI CCGG 2 cut(s) 373, 1073
MspR9I CCNGG 1 cut(s) 573
MvaI CCWGG 1 cut(s) 573
MwoI GCNNNNNNNGC 6 cut(s) 22, 359, 506, 1632, 1736, 1999
NcoI CCATGG 3 cut(s) 150, 1249, 1650
NdeI CATATG 2 cut(s) 1969, 2005
NdeII GATC 6 cut(s) 463, 754, 774, 1132, 1283, 1669
NlaIII CATG 9 cut(s) 154, 504, 702, 1019, 1123, 1253, 1654, 1783, 1981
NlaIV GGNNCC 3 cut(s) 84, 496, 727
NmuCI GTSAC 4 cut(s) 701, 1553, 1819, 1887
NsiI ATGCAT 1 cut(s) 1969
NspI RCATGY 2 cut(s) 504, 1981
NspV TTCGAA 1 cut(s) 1209
PaeI GCATGC 2 cut(s) 504, 1981
PaeR7I CTCGAG 1 cut(s) 1802
PceI AGGCCT 1 cut(s) 1774
PciSI GCTCTTC 1 cut(s) 394
PcsI WCGNNNNNNNCGW 1 cut(s) 1908
PdmI GAANNNNTTC 1 cut(s) 423
PfeI GAWTC 2 cut(s) 664, 1026
PkrI GCNGC 1 cut(s) 347
PleI GAGTC 2 cut(s) 472, 550
PpsI GAGTC 2 cut(s) 472, 550
PpuMI RGGWCCY 1 cut(s) 83
Psp124BI GAGCTC 1 cut(s) 1803
Psp1406I AACGTT 1 cut(s) 71
Psp5II RGGWCCY 1 cut(s) 83
Psp6I CCWGG 1 cut(s) 571
PspFI CCCAGC 1 cut(s) 529
PspGI CCWGG 1 cut(s) 571
PspN4I GGNNCC 3 cut(s) 84, 496, 727
PspPI GGNCC 3 cut(s) 83, 444, 1729
PspPPI RGGWCCY 1 cut(s) 83
PspXI VCTCGAGB 1 cut(s) 1802
PstI CTGCAG 2 cut(s) 347, 1995
PstNI CAGNNNCTG 1 cut(s) 1472
PsuI RGATCY 1 cut(s) 463
RsaI GTAC 5 cut(s) 593, 896, 1111, 1877, 1957
RsaNI GTAC 5 cut(s) 592, 895, 1110, 1876, 1956
RseI CAYNNNNRTG 6 cut(s) 321, 600, 1179, 1181, 1383, 1640
SacI GAGCTC 1 cut(s) 1803
SapI GCTCTTC 1 cut(s) 394
SaqAI TTAA 8 cut(s) 222, 261, 336, 438, 879, 1143, 1170, 1845
SatI GCNGC 1 cut(s) 346
Sau3AI GATC 6 cut(s) 463, 754, 774, 1132, 1283, 1669
Sau96I GGNCC 3 cut(s) 83, 444, 1729
ScaI AGTACT 2 cut(s) 896, 1877
SchI GAGTC 2 cut(s) 472, 551
ScrFI CCNGG 1 cut(s) 573
SduI GDGCHC 3 cut(s) 1269, 1494, 1803
SfaNI GCATC 5 cut(s) 753, 1613, 1644, 1736, 1989
SfcI CTRYAG 3 cut(s) 343, 546, 1991
Sfr274I CTCGAG 1 cut(s) 1802
SfuI TTCGAA 1 cut(s) 1209
SinI GGWCC 1 cut(s) 83
SlaI CTCGAG 1 cut(s) 1802
SmiMI CAYNNNNRTG 6 cut(s) 321, 600, 1179, 1181, 1383, 1640
SmlI CTYRAG 2 cut(s) 992, 1802
SmoI CTYRAG 2 cut(s) 992, 1802
SpeI ACTAGT 2 cut(s) 1112, 1228
SphI GCATGC 2 cut(s) 504, 1981
SseBI AGGCCT 1 cut(s) 1774
SsiI CCGC 3 cut(s) 498, 981, 2010
SspI AATATT 1 cut(s) 1402
SspMI CTAG 8 cut(s) 57, 393, 1113, 1229, 1511, 1580, 1851, 1938
SstI GAGCTC 1 cut(s) 1803
StuI AGGCCT 1 cut(s) 1774
StyD4I CCNGG 1 cut(s) 571
StyI CCWWGG 5 cut(s) 150, 832, 1030, 1249, 1650
TaaI ACNGT 4 cut(s) 1054, 1084, 1717, 1904
TaiI ACGT 4 cut(s) 74, 1560, 1911, 2020
TaqI TCGA 8 cut(s) 11, 294, 759, 956, 1103, 1209, 1381, 1803
TatI WGTACW 2 cut(s) 894, 1875
TfiI GAWTC 2 cut(s) 664, 1026
Tru1I TTAA 8 cut(s) 222, 261, 336, 438, 879, 1143, 1170, 1845
Tru9I TTAA 8 cut(s) 222, 261, 336, 438, 879, 1143, 1170, 1845
TscAI CASTG 6 cut(s) 223, 277, 569, 946, 1089, 1957
TseFI GTSAC 4 cut(s) 701, 1553, 1819, 1887
TseI GCWGC 1 cut(s) 345
Tsp45I GTSAC 4 cut(s) 701, 1553, 1819, 1887
TspGWI ACGGA 1 cut(s) 1824
TspRI CASTG 6 cut(s) 223, 277, 569, 946, 1089, 1957
VpaK11BI GGWCC 1 cut(s) 83
XapI RAATTY 2 cut(s) 1238, 1674
XceI RCATGY 2 cut(s) 504, 1981
XcmI CCANNNNNNNNNTGG 1 cut(s) 569
XhoI CTCGAG 1 cut(s) 1802
XmiI GTMKAC 1 cut(s) 1233
XmnI GAANNNNTTC 1 cut(s) 423
XspI CTAG 8 cut(s) 57, 393, 1113, 1229, 1511, 1580, 1851, 1938
ZrmI AGTACT 2 cut(s) 896, 1877
Zsp2I ATGCAT 1 cut(s) 1969
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.