RchiOBHm_Chr5g0016641

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000066
Physical Location & Seq
Reverse (-)
11463829 .. 11466296
2468 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29701

Sequence Viewer

Length: 1821 bp
ATGACCGGATGCATGTCCATATGTGACAACCTTGACAGCGTTGAACAGTCTTGCTCCGGGGTTGGGTGTTGCCTAACTGACATCCCTAGAGGACTTGAGAACTACACTGTCAGACTGGATAGCTACTATAATCATACATACATACTGGACTTCAATCCATGCAGCTATGCTTTCATTGTGGAAGAAGGCAAGTTTACCTTTTCCCCGAATACAAGTTTTAACGACCTCTCCAACACTGAAGAGCTTCCCATGATTCTTAATTGGGAAATTGGGGAAGGGCCATGTGATGAAGCTCAAAAGAAAGATGATTATGCATGCAAGGCAAATAGTAAGTGTGTCAATCGGACCATCAGCGATGAGCTATCTGGTTATATTTGTCACTGCCAGCCGGGTTATCAAGGGAACCCTTACCTCCCTGATGGTTGCCAAGATATTGATGAGTGCAAGGTTTCAAACCCATGCACCAATGGAAGGTGTGCAAATTCACCTGGAAGTTACTCTTGTTTGTGTGACAAAGGGTACAGAAGCAATGGAACAAATGATCAAAGTTGTATTAAGGAGATTCCTAAAAATCAACCAGAGACCACGCTCCTGCTTATTGTATCATTGAGTATAACTGGCGGATTCTTGGTTCTATTTGTTGGAATTTCATGGATATGTAGCGGAATAAAGAAAAGAAAATTCATGAAACTCAAAGAAAAGTACTTTAAAGAGAATGGTGGCTTATTGTTACTACAGAAACTCGCTAATCATGGAGGCTCGGTGGAGACAACAAAAATATTCACCACAGAAGAACTAGAGAAGGCAACAAACAATTACCATGAGAGTAGAGTCCTTGGAGAAGGAGGCTACGGAACAGTTTATAAAGGGGTACTACTTGACAACAAAGTGGTTGCAATAAAGAAGTCCAAAGGTGGTACCTCGATCCAGAGTGAACAGTTTGTTAATGAGGTGGTTGTTCTTTCCCAAATCAACCACAGAAATGTGGTGAAGCTACTAGGTTGTTGTTTAGAGACAGCAGTACCTTTACTAGTATATGAGTTTGTCACCCATGGCACTTTATATGAGCACATTCATAGAAAAAGATCACTACTGTCATTCGAATTACGAATGAAGATAGCTGTTGAAACAGCAGGAGCACTAGCATACTTACACTCATCTATATCCACGCCAATCATACATCGAGATGTGAAAGCAGCAAATATACTCCTAGATGATAATTATACAGCTAAAGTGTCGGACTTTGGAGCTTCACGGTTGGTTCCTTCAGGTCAAACTGAAATACAAACTTTAGTGCTCGGGACATATGGATACCTAGACCCAGAATATTTGCAGTCAAACCAACTAACAGAAAAAAGTGATGTCTACAGCTTTGGAGTTGTCCTAGTGGAGCTACTAACAAGCAAAGTGGCATTTTCTAATGACAGATGCCTAGCAAGCATCTTTGTTTCTTCCATGGATGAAGATTGCTTAAAACAAATTCTTGATGGTGCCATAGTGAACGAGGGAAACATTGAGACAGCAGAAAACGTGGCGAGTATCGCAAAAAGATGTTTGAGGGTAAAAGGGGAGGAAAGGCCTACCATGAAAGAAGTTGCCATGGAGTTAGAGGGAATGACAATTATGGCAAAGCATCCATGGGGAAAGAATGCCATGTTTTGTCCAGAGGAGAATGAGTACTTGCTTGGGTCACCTTCAGACAGTAATGTTATGGACGTTAGAGGAGGTGATCGCGATTCTAGTGGTTTAACTACTGGTATGACGACTGGGTATGACAGCATGCAAATCCAGATGTTGATGCCACGTGGTGATGGGCGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK

Protein Analysis

606

Amino Acids

66.85

Weight (kDa)

5.21

Isoelectric Point (pI)

37.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EGF_CA PF07645 144 - 184 9.3e-08 Calcium-binding EGF domain
Pkinase PF00069 273 - 532 6e-51 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 276 - 536 3.7e-50 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21210 AT1G21230 AT1G21240 AT1G21240 AT1G21245 AT1G21250 AT1G21270 AT1G22720 AT3G25490
fragaria_vesca FvH4_2g35051 FvH4_2g35051 FvH4_2g35060 FvH4_3g10281 FvH4_3g10285 FvH4_3g10286 FvH4_3g10311 FvH4_3g10311 FvH4_3g10320 FvH4_3g10332 FvH4_3g10340 FvH4_3g10350 FvH4_3g10360 FvH4_3g10390 FvH4_3g10412 FvH4_3g10413 FvH4_3g10461 FvH4_3g10462 FvH4_3g10470 FvH4_3g10510 FvH4_3g10520 FvH4_3g10530 FvH4_3g10550 FvH4_3g10560 FvH4_3g10611 FvH4_3g11730 FvH4_6g53140 FvH4_6g53142 FvH4_7g31880 FvH4_7g31910 FvH4_7g31920
malus_domestica MD05G1269900.v1.1 MD05G1270000.v1.1 MD05G1270200.v1.1 MD05G1270400.v1.1 MD05G1270500.v1.1 MD05G1270600.v1.1 MD05G1270800.v1.1 MD05G1270900.v1.1 MD07G1183800.v1.1 MD08G1107600.v1.1 MD10G1248500.v1.1 MD10G1249200.v1.1 MD10G1249500.v1.1 MD10G1250000.v1.1 MD10G1250500.v1.1 MD10G1250900.v1.1 MD10G1251200.v1.1 MD10G1251400.v1.1 MD13G1004600.v1.1 MD15G1088400.v1.1
prunus_persica Prupe.1G442400_v2.0.a1 Prupe.1G442500_v2.0.a1 Prupe.2G223400_v2.0.a1 Prupe.4G091900_v2.0.a1 Prupe.4G093200_v2.0.a1 Prupe.4G093300_v2.0.a1 Prupe.4G093400_v2.0.a1 Prupe.4G093500_v2.0.a1 Prupe.4G094100_v2.0.a1 Prupe.4G094200_v2.0.a1 Prupe.4G094300_v2.0.a1 Prupe.6G358300_v2.0.a1
pyrus_communis pycom08g08990 pycom10g20660 pycom10g20670 pycom10g20680 pycom10g20710 pycom10g20740 pycom13g00400 pycom15g08290
rosa_chinensis RchiOBHm_Chr1g0326861 RchiOBHm_Chr1g0326871 RchiOBHm_Chr1g0331391 RchiOBHm_Chr1g0336911 RchiOBHm_Chr1g0364001 RchiOBHm_Chr2g0174541 RchiOBHm_Chr2g0175041 RchiOBHm_Chr2g0175101 RchiOBHm_Chr2g0175121 RchiOBHm_Chr3g0486331 RchiOBHm_Chr5g0015581 RchiOBHm_Chr5g0016551 RchiOBHm_Chr5g0016561 RchiOBHm_Chr5g0016581 RchiOBHm_Chr5g0016601 RchiOBHm_Chr5g0016611 RchiOBHm_Chr5g0016641 RchiOBHm_Chr5g0016661 RchiOBHm_Chr5g0016691 RchiOBHm_Chr5g0016701 RchiOBHm_Chr5g0016731 RchiOBHm_Chr5g0016741 RchiOBHm_Chr5g0016811 RchiOBHm_Chr5g0016841 RchiOBHm_Chr5g0016871 RchiOBHm_Chr5g0016881 RchiOBHm_Chr5g0016931 RchiOBHm_Chr5g0017021 RchiOBHm_Chr5g0017041 RchiOBHm_Chr5g0017071 RchiOBHm_Chr5g0017091 RchiOBHm_Chr5g0017121 RchiOBHm_Chr5g0017141 RchiOBHm_Chr5g0017291 RchiOBHm_Chr5g0017321 RchiOBHm_Chr5g0017401 RchiOBHm_Chr5g0019001 RchiOBHm_Chr5g0019071 RchiOBHm_Chr5g0028941 RchiOBHm_Chr5g0052141 RchiOBHm_Chr5g0054721 RchiOBHm_Chr5g0054801 RchiOBHm_Chr5g0054931 RchiOBHm_Chr5g0054941 RchiOBHm_Chr6g0259291 RchiOBHm_Chr6g0292991 RchiOBHm_Chr6g0306911
rosa_laevigata RLG00000009465 RLG00000009468 RLG00000010755 RLG00000011976 RLG00000014541 RLG00000014542 RLG00000015169 RLG00000022308 RLG00000027529 RLG00000029324 RLG00000030244 RLG00000030245 RLG00000032210 RLG00000032211 RLG00000032257 RLG00000032259 RLG00000032260 RLG00000032261 RLG00000032262 RLG00000032268 RLG00000032269 RLG00000032272 RLG00000032282 RLG00000032283 RLG00000032308 RLG00000032413 RLG00000033129 RLG00000034945
rosa_multiflora Rmu_co8241929.1_g000001 Rmu_co8269227.1_g000001 Rmu_co8461259.1_g000001 Rmu_co8495385.1_g000001 Rmu_sc0000028.1_g000003 Rmu_sc0000083.1_g000009 Rmu_sc0000083.1_g000010 Rmu_sc0000083.1_g000031 Rmu_sc0000083.1_g000043 Rmu_sc0000083.1_g000046 Rmu_sc0000083.1_g000047 Rmu_sc0000563.1_g000024 Rmu_sc0000842.1_g000012 Rmu_sc0001235.1_g000001 Rmu_sc0001235.1_g000005 Rmu_sc0001235.1_g000009 Rmu_sc0001235.1_g000015 Rmu_sc0001235.1_g000016 Rmu_sc0001235.1_g000017 Rmu_sc0001473.1_g000036 Rmu_sc0001607.1_g000016 Rmu_sc0001788.1_g000006 Rmu_sc0002018.1_g000008 Rmu_sc0002028.1_g000001 Rmu_sc0002680.1_g000011 Rmu_sc0002798.1_g000023 Rmu_sc0002886.1_g000023 Rmu_sc0002989.1_g000012 Rmu_sc0003064.1_g000009 Rmu_sc0003382.1_g000006 Rmu_sc0003630.1_g000002 Rmu_sc0003630.1_g000047 Rmu_sc0003630.1_g000066 Rmu_sc0003630.1_g000067 Rmu_sc0003649.1_g000010 Rmu_sc0003688.1_g000005 Rmu_sc0003920.1_g000007 Rmu_sc0004765.1_g000007 Rmu_sc0004765.1_g000008 Rmu_sc0004920.1_g000011 Rmu_sc0005223.1_g000008 Rmu_sc0005223.1_g000010 Rmu_sc0005223.1_g000015 Rmu_sc0005223.1_g000016 Rmu_sc0005319.1_g000014 Rmu_sc0005319.1_g000015 Rmu_sc0005319.1_g000018 Rmu_sc0005319.1_g000019 Rmu_sc0005319.1_g000025 Rmu_sc0005418.1_g000007 Rmu_sc0005418.1_g000012 Rmu_sc0006106.1_g000011 Rmu_sc0006914.1_g000012 Rmu_sc0007023.1_g000001 Rmu_sc0007662.1_g000001 Rmu_sc0007920.1_g000002 Rmu_sc0007920.1_g000013 Rmu_sc0008053.1_g000016 Rmu_sc0008053.1_g000017 Rmu_sc0008053.1_g000018 Rmu_sc0008053.1_g000023 Rmu_sc0008053.1_g000050 Rmu_sc0009042.1_g000007 Rmu_sc0009042.1_g000008 Rmu_sc0010642.1_g000008 Rmu_sc0011109.1_g000002 Rmu_sc0011314.1_g000006 Rmu_sc0011974.1_g000006 Rmu_sc0012179.1_g000009 Rmu_sc0015169.1_g000001 Rmu_sc0016368.1_g000002 Rmu_sc0022659.1_g000001 Rmu_sc0027371.1_g000001 Rmu_sc0028213.1_g000001 Rmu_sc0034526.1_g000001 Rmu_sc0039366.1_g000002 Rmu_ssc0000050.1_g000084 Rmu_ssc0000050.1_g000088 Rmu_ssc0000167.1_g000017
rosa_roxburghii Rroxscaffold_1G00011340 Rroxscaffold_1G00025710 Rroxscaffold_1G00025870 Rroxscaffold_1G00026070 Rroxscaffold_1G00026170 Rroxscaffold_1G00026200 Rroxscaffold_1G00026270 Rroxscaffold_1G00050950 Rroxscaffold_1G00058900 Rroxscaffold_1G00060040 Rroxscaffold_1G00060060 Rroxscaffold_1G00060070 Rroxscaffold_1G00060080 Rroxscaffold_1G00060110 Rroxscaffold_1G00060120 Rroxscaffold_1G00060140 Rroxscaffold_1G00060170 Rroxscaffold_1G00060210 Rroxscaffold_1G00060220 Rroxscaffold_1G00060240 Rroxscaffold_1G00060250 Rroxscaffold_1G00060260 Rroxscaffold_1G00060300 Rroxscaffold_1G00060320 Rroxscaffold_1G00060330 Rroxscaffold_1G00060900 Rroxscaffold_1G00060920 Rroxscaffold_1G00073980 Rroxscaffold_2G00077560 Rroxscaffold_2G00077580 Rroxscaffold_2G00146990 Rroxscaffold_3G00236200 Rroxscaffold_3G00236210 Rroxscaffold_3G00236220 Rroxscaffold_4G00292760 Rroxscaffold_4G00315200 Rroxscaffold_4G00315240 Rroxscaffold_4G00326240 Rroxscaffold_4G00328980 Rroxscaffold_5G00341890 Rroxscaffold_7G00161350 Rroxscaffold_7G00174490 Rroxscaffold_7G00206550
rosa_rugosa Rorug01G0093000 Rorug01G0093100 Rorug01G0128800 Rorug01G0312000 Rorug01G0312000 Rorug02G0581100 Rorug03G0224600 Rorug05G0028500 Rorug05G0032700 Rorug05G0032800 Rorug05G0032900 Rorug05G0032900 Rorug05G0033000 Rorug05G0033100 Rorug05G0033300 Rorug05G0033400 Rorug05G0033900 Rorug05G0034900 Rorug05G0035000 Rorug05G0046900 Rorug05G0047500 Rorug05G0047500 Rorug05G0111600 Rorug05G0287900 Rorug05G0288700 Rorug05G0586900 Rorug06G0231700 Rorug06G0354300 Rorug06G0354400
rosa_samantha Rh1DG122200 Rh2DG686800 Rh5BG136600 Rh6AG344500 Rh6BG351900 Rh6CG359000 Rh6CG359100 Rh6CG387600 Rh6CG480500 Rh6DG344600 Rh6DG467400
rosa_wichuraiana Rw0G010250 Rw0G014820 Rw0G021400 Rw0G023100 Rw1G009450 Rw1G012100 Rw1G012180 Rw1G028360 Rw2G054260 Rw3G024370 Rw3G024420 Rw3G024470 Rw5G010850 Rw5G011010 Rw5G011020 Rw5G011050 Rw5G011080 Rw5G011120 Rw5G012300 Rw5G018470 Rw5G033830 Rw6G008930 Rw6G030070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 864
Acc65I GGTACC 1 cut(s) 917
AccB1I GGYRCC 2 cut(s) 917, 1490
AccI GTMKAC 1 cut(s) 1365
AccII CGCG 1 cut(s) 1734
AciI CCGC 2 cut(s) 621, 663
AclWI GGATC 1 cut(s) 919
AcsI RAATTY 4 cut(s) 481, 645, 680, 1479
AcuI CTGAAG 3 cut(s) 258, 1251, 1680
AcvI CACGTG 1 cut(s) 1805
AdeI CACNNNGTG 1 cut(s) 1808
AfaI GTAC 6 cut(s) 521, 704, 873, 919, 1023, 1679
AfiI CCNNNNNNNGG 2 cut(s) 63, 471
AgsI TTSAA 4 cut(s) 44, 154, 453, 1127
AhlI ACTAGT 1 cut(s) 1030
AjnI CCWGG 1 cut(s) 487
AloI GAACNNNNNNTCC 2 cut(s) 615, 647
Alw21I GWGCWC 3 cut(s) 1071, 1141, 1299
Alw26I GTCTC 4 cut(s) 575, 761, 1007, 1511
AlwI GGATC 1 cut(s) 919
Ama87I CYCGRG 1 cut(s) 1298
AoxI GGCC 2 cut(s) 278, 1577
ApeKI GCWGC 2 cut(s) 162, 1196
ApoI RAATTY 4 cut(s) 481, 645, 680, 1479
Asp700I GAANNNNTTC 1 cut(s) 243
Asp718I GGTACC 1 cut(s) 917
AspS9I GGNCC 2 cut(s) 278, 345
AsuC2I CCSGG 2 cut(s) 58, 390
AsuHPI GGTGA 7 cut(s) 477, 775, 1000, 1039, 1683, 1739, 1820
AsuII TTCGAA 1 cut(s) 1101
AvaI CYCGRG 1 cut(s) 1298
AvaII GGWCC 1 cut(s) 345
BaeI ACNNNNGTAYC 2 cut(s) 1005, 1038
BanI GGYRCC 2 cut(s) 917, 1490
BbrPI CACGTG 1 cut(s) 1805
Bbv12I GWGCWC 3 cut(s) 1071, 1141, 1299
BbvI GCAGC 2 cut(s) 174, 1208
BccI CCATC 4 cut(s) 356, 413, 1481, 1805
BciT130I CCWGG 1 cut(s) 489
BciVI GTATCC 1 cut(s) 1304
BclI TGATCA 1 cut(s) 541
BcnI CCSGG 2 cut(s) 58, 390
BcoDI GTCTC 4 cut(s) 575, 761, 1007, 1511
BcuI ACTAGT 1 cut(s) 1030
BfmI CTRYAG 2 cut(s) 734, 1366
BfuI GTATCC 1 cut(s) 1304
BisI GCNGC 2 cut(s) 163, 1197
BlsI GCNGC 2 cut(s) 164, 1198
BmcAI AGTACT 2 cut(s) 704, 1679
Bme1390I CCNGG 3 cut(s) 58, 390, 489
Bme18I GGWCC 1 cut(s) 345
BmeT110I CYCGRG 1 cut(s) 1298
BmgT120I GGNCC 2 cut(s) 278, 345
BmiI GGNNCC 4 cut(s) 404, 919, 1263, 1492
BmrFI CCNGG 3 cut(s) 58, 390, 489
BmrI ACTGGG 1 cut(s) 1776
BmsI GCATC 4 cut(s) 1418, 1449, 1642, 1788
BmuI ACTGGG 1 cut(s) 1776
Bpu14I TTCGAA 1 cut(s) 1101
BpuEI CTTGAG 1 cut(s) 116
BpuMI CCSGG 2 cut(s) 58, 390
BsaAI YACGTR 1 cut(s) 1805
BsaI GGTCTC 1 cut(s) 575
BsaJI CCNNGG 6 cut(s) 57, 835, 1051, 1455, 1599, 1637
BsaWI WCCGGW 1 cut(s) 5
BsaXI ACNNNNNCTCC 6 cut(s) 212, 242, 747, 777, 1661, 1691
Bsc4I CCNNNNNNNGG 2 cut(s) 63, 471
Bse1I ACTGG 5 cut(s) 120, 150, 622, 1759, 1771
Bse3DI GCAATG 1 cut(s) 535
BseBI CCWGG 1 cut(s) 489
BseDI CCNNGG 6 cut(s) 57, 835, 1051, 1455, 1599, 1637
BseGI GGATG 4 cut(s) 14, 81, 1465, 1633
BseLI CCNNNNNNNGG 2 cut(s) 63, 471
BseMI GCAATG 1 cut(s) 535
BseNI ACTGG 5 cut(s) 120, 150, 622, 1759, 1771
BseRI GAGGAG 2 cut(s) 1682, 1737
BseXI GCAGC 2 cut(s) 174, 1208
Bsh1236I CGCG 1 cut(s) 1734
BshFI GGCC 2 cut(s) 280, 1579
BshNI GGYRCC 2 cut(s) 917, 1490
BsiHKAI GWGCWC 3 cut(s) 1071, 1141, 1299
BsiHKCI CYCGRG 1 cut(s) 1298
BsiSI CCGG 3 cut(s) 6, 57, 389
BslFI GGGAC 1 cut(s) 1315
BslI CCNNNNNNNGG 2 cut(s) 63, 471
BsmAI GTCTC 4 cut(s) 575, 761, 1007, 1511
BsmFI GGGAC 1 cut(s) 1315
BsmI GAATGC 1 cut(s) 1654
BsnI GGCC 2 cut(s) 280, 1579
Bso31I GGTCTC 1 cut(s) 575
BsoBI CYCGRG 1 cut(s) 1298
Bsp119I TTCGAA 1 cut(s) 1101
Bsp1286I GDGCHC 3 cut(s) 1071, 1141, 1299
Bsp143I GATC 4 cut(s) 541, 924, 1085, 1729
Bsp19I CCATGG 4 cut(s) 1051, 1455, 1599, 1637
Bsp68I TCGCGA 1 cut(s) 1734
BspACI CCGC 2 cut(s) 621, 663
BspANI GGCC 2 cut(s) 280, 1579
BspFNI CGCG 1 cut(s) 1734
BspHI TCATGA 1 cut(s) 684
BspLI GGNNCC 4 cut(s) 404, 919, 1263, 1492
BspPI GGATC 1 cut(s) 919
BspQI GCTCTTC 1 cut(s) 234
BspT104I TTCGAA 1 cut(s) 1101
BspT107I GGYRCC 2 cut(s) 917, 1490
BspTNI GGTCTC 1 cut(s) 575
BsrDI GCAATG 1 cut(s) 535
BsrI ACTGG 5 cut(s) 120, 150, 622, 1759, 1771
BssECI CCNNGG 6 cut(s) 57, 835, 1051, 1455, 1599, 1637
BssMI GATC 4 cut(s) 541, 924, 1085, 1729
BssT1I CCWWGG 5 cut(s) 835, 1051, 1455, 1599, 1637
Bst2UI CCWGG 1 cut(s) 489
Bst4CI ACNGT 7 cut(s) 48, 109, 859, 939, 1095, 1257, 1703
Bst6I CTCTTC 1 cut(s) 234
BstBAI YACGTR 1 cut(s) 1805
BstBI TTCGAA 1 cut(s) 1101
BstC8I GCNNGC 4 cut(s) 316, 386, 1438, 1781
BstDSI CCRYGG 4 cut(s) 1051, 1455, 1599, 1637
BstEII GGTNACC 1 cut(s) 1689
BstF5I GGATG 4 cut(s) 14, 81, 1465, 1633
BstFNI CGCG 1 cut(s) 1734
BstKTI GATC 4 cut(s) 544, 927, 1088, 1732
BstMAI GTCTC 4 cut(s) 575, 761, 1007, 1511
BstMBI GATC 4 cut(s) 541, 924, 1085, 1729
BstMWI GCNNNNNNNGC 3 cut(s) 320, 1437, 1541
BstNI CCWGG 1 cut(s) 489
BstNSI RCATGY 3 cut(s) 16, 318, 1783
BstPI GGTNACC 1 cut(s) 1689
BstSCI CCNGG 3 cut(s) 56, 388, 487
BstSFI CTRYAG 2 cut(s) 734, 1366
BstUI CGCG 1 cut(s) 1734
BstV1I GCAGC 2 cut(s) 174, 1208
BsuI GTATCC 1 cut(s) 1304
BsuRI GGCC 2 cut(s) 280, 1579
BtgI CCRYGG 4 cut(s) 1051, 1455, 1599, 1637
BtgZI GCGATG 1 cut(s) 369
BtsCI GGATG 4 cut(s) 14, 81, 1465, 1633
BtsI GCAGTG 1 cut(s) 379
BtsIMutI CAGTG 3 cut(s) 105, 234, 379
BtuMI TCGCGA 1 cut(s) 1734
Cac8I GCNNGC 4 cut(s) 316, 386, 1438, 1781
CciI TCATGA 1 cut(s) 684
Cfr13I GGNCC 2 cut(s) 278, 345
Csp6I GTAC 6 cut(s) 520, 703, 872, 918, 1022, 1678
CspCI CAANNNNNGTGG 2 cut(s) 1389, 1424
CviQI GTAC 6 cut(s) 520, 703, 872, 918, 1022, 1678
DpnI GATC 4 cut(s) 543, 926, 1087, 1731
DpnII GATC 4 cut(s) 541, 924, 1085, 1729
DraI TTTAAA 1 cut(s) 709
DraIII CACNNNGTG 1 cut(s) 1808
Eam1104I CTCTTC 1 cut(s) 234
EarI CTCTTC 1 cut(s) 234
EciI GGCGGA 1 cut(s) 636
Eco130I CCWWGG 5 cut(s) 835, 1051, 1455, 1599, 1637
Eco147I AGGCCT 1 cut(s) 1579
Eco31I GGTCTC 1 cut(s) 575
Eco47I GGWCC 1 cut(s) 345
Eco57I CTGAAG 3 cut(s) 258, 1251, 1680
Eco72I CACGTG 1 cut(s) 1805
Eco88I CYCGRG 1 cut(s) 1298
Eco91I GGTNACC 1 cut(s) 1689
EcoO65I GGTNACC 1 cut(s) 1689
EcoRII CCWGG 1 cut(s) 487
EcoT14I CCWWGG 5 cut(s) 835, 1051, 1455, 1599, 1637
EcoT22I ATGCAT 2 cut(s) 14, 316
ErhI CCWWGG 5 cut(s) 835, 1051, 1455, 1599, 1637
FalI AAGNNNNNCTT 4 cut(s) 182, 214, 484, 516
FaqI GGGAC 1 cut(s) 1315
FauNDI CATATG 2 cut(s) 20, 1306
FbaI TGATCA 1 cut(s) 541
FblI GTMKAC 1 cut(s) 1365
Fnu4HI GCNGC 2 cut(s) 163, 1197
FokI GGATG 4 cut(s) 21, 68, 1472, 1620
Fsp4HI GCNGC 2 cut(s) 163, 1197
GluI GCNGC 2 cut(s) 163, 1197
HaeIII GGCC 2 cut(s) 280, 1579
HapII CCGG 3 cut(s) 6, 57, 389
HinfI GANTC 5 cut(s) 253, 562, 624, 831, 1736
HpaII CCGG 3 cut(s) 6, 57, 389
HphI GGTGA 7 cut(s) 477, 775, 1000, 1039, 1683, 1739, 1820
Hpy166II GTNNAC 4 cut(s) 195, 935, 1366, 1501
Hpy188I TCNGA 4 cut(s) 113, 345, 1240, 1699
Hpy188III TCNNGA 8 cut(s) 685, 928, 1184, 1300, 1484, 1664, 1733, 1789
Hpy8I GTNNAC 4 cut(s) 195, 935, 1366, 1501
HpyAV CCTTC 7 cut(s) 179, 269, 465, 796, 836, 1275, 1704
HpyCH4III ACNGT 7 cut(s) 48, 109, 859, 939, 1095, 1257, 1703
HpyCH4IV ACGT 3 cut(s) 1530, 1716, 1804
HpyF10VI GCNNNNNNNGC 3 cut(s) 320, 1437, 1541
HpySE526I ACGT 3 cut(s) 1530, 1716, 1804
KpnI GGTACC 1 cut(s) 921
Ksp22I TGATCA 1 cut(s) 541
Kzo9I GATC 4 cut(s) 541, 924, 1085, 1729
LguI GCTCTTC 1 cut(s) 234
LmnI GCTCC 5 cut(s) 59, 594, 1136, 1247, 1390
Lsp1109I GCAGC 2 cut(s) 174, 1208
LweI GCATC 4 cut(s) 1418, 1449, 1642, 1788
MaeII ACGT 3 cut(s) 1530, 1716, 1804
MaeIII GTNAC 7 cut(s) 23, 377, 494, 509, 729, 1045, 1689
MalI GATC 4 cut(s) 543, 926, 1087, 1731
MboI GATC 4 cut(s) 541, 924, 1085, 1729
MboII GAAGA 6 cut(s) 194, 251, 803, 1126, 1443, 1475
MhlI GDGCHC 3 cut(s) 1071, 1141, 1299
MlyI GAGTC 1 cut(s) 840
MmeI TCCRAC 3 cut(s) 255, 622, 1218
Mph1103I ATGCAT 2 cut(s) 14, 316
MroXI GAANNNNTTC 1 cut(s) 243
MseI TTAA 7 cut(s) 219, 258, 555, 708, 945, 1472, 1748
MslI CAYNNNNRTG 3 cut(s) 655, 981, 1185
MspI CCGG 3 cut(s) 6, 57, 389
MspR9I CCNGG 3 cut(s) 58, 390, 489
Mva1269I GAATGC 1 cut(s) 1654
MvaI CCWGG 1 cut(s) 489
MvnI CGCG 1 cut(s) 1734
MwoI GCNNNNNNNGC 3 cut(s) 320, 1437, 1541
NciI CCSGG 2 cut(s) 58, 390
NcoI CCATGG 4 cut(s) 1051, 1455, 1599, 1637
NdeI CATATG 2 cut(s) 20, 1306
NdeII GATC 4 cut(s) 541, 924, 1085, 1729
NlaIV GGNNCC 4 cut(s) 404, 919, 1263, 1492
NmuCI GTSAC 5 cut(s) 23, 377, 509, 1045, 1689
NruI TCGCGA 1 cut(s) 1734
NsiI ATGCAT 2 cut(s) 14, 316
NspI RCATGY 3 cut(s) 16, 318, 1783
NspV TTCGAA 1 cut(s) 1101
PaeI GCATGC 2 cut(s) 318, 1783
PagI TCATGA 1 cut(s) 684
PceI AGGCCT 1 cut(s) 1579
PciSI GCTCTTC 1 cut(s) 234
PctI GAATGC 1 cut(s) 1654
PdmI GAANNNNTTC 1 cut(s) 243
PfeI GAWTC 4 cut(s) 253, 562, 624, 1736
PkrI GCNGC 2 cut(s) 164, 1198
PleI GAGTC 1 cut(s) 839
PmaCI CACGTG 1 cut(s) 1805
PmlI CACGTG 1 cut(s) 1805
PpsI GAGTC 1 cut(s) 839
Ppu21I YACGTR 1 cut(s) 1805
PsiI TTATAA 1 cut(s) 864
Psp6I CCWGG 1 cut(s) 487
PspCI CACGTG 1 cut(s) 1805
PspEI GGTNACC 1 cut(s) 1689
PspGI CCWGG 1 cut(s) 487
PspN4I GGNNCC 4 cut(s) 404, 919, 1263, 1492
PspPI GGNCC 2 cut(s) 278, 345
RruI TCGCGA 1 cut(s) 1734
RsaI GTAC 6 cut(s) 521, 704, 873, 919, 1023, 1679
RsaNI GTAC 6 cut(s) 520, 703, 872, 918, 1022, 1678
RseI CAYNNNNRTG 3 cut(s) 655, 981, 1185
SapI GCTCTTC 1 cut(s) 234
SaqAI TTAA 7 cut(s) 219, 258, 555, 708, 945, 1472, 1748
SatI GCNGC 2 cut(s) 163, 1197
Sau3AI GATC 4 cut(s) 541, 924, 1085, 1729
Sau96I GGNCC 2 cut(s) 278, 345
ScaI AGTACT 2 cut(s) 704, 1679
SchI GAGTC 1 cut(s) 840
ScrFI CCNGG 3 cut(s) 58, 390, 489
SduI GDGCHC 3 cut(s) 1071, 1141, 1299
SfaNI GCATC 4 cut(s) 1418, 1449, 1642, 1788
SfcI CTRYAG 2 cut(s) 734, 1366
SfuI TTCGAA 1 cut(s) 1101
SinI GGWCC 1 cut(s) 345
SmiMI CAYNNNNRTG 3 cut(s) 655, 981, 1185
SmlI CTYRAG 1 cut(s) 95
SmoI CTYRAG 1 cut(s) 95
SpeI ACTAGT 1 cut(s) 1030
SphI GCATGC 2 cut(s) 318, 1783
SseBI AGGCCT 1 cut(s) 1579
SsiI CCGC 2 cut(s) 621, 663
SspI AATATT 2 cut(s) 780, 1328
StuI AGGCCT 1 cut(s) 1579
StyD4I CCNGG 3 cut(s) 56, 388, 487
StyI CCWWGG 5 cut(s) 835, 1051, 1455, 1599, 1637
TaaI ACNGT 7 cut(s) 48, 109, 859, 939, 1095, 1257, 1703
TaiI ACGT 3 cut(s) 1533, 1719, 1807
TaqI TCGA 3 cut(s) 923, 1101, 1183
TatI WGTACW 2 cut(s) 702, 1677
TfiI GAWTC 4 cut(s) 253, 562, 624, 1736
Tru1I TTAA 7 cut(s) 219, 258, 555, 708, 945, 1472, 1748
Tru9I TTAA 7 cut(s) 219, 258, 555, 708, 945, 1472, 1748
TscAI CASTG 3 cut(s) 112, 241, 386
TseFI GTSAC 5 cut(s) 23, 377, 509, 1045, 1689
TseI GCWGC 2 cut(s) 162, 1196
Tsp45I GTSAC 5 cut(s) 23, 377, 509, 1045, 1689
TspDTI ATGAA 9 cut(s) 163, 303, 639, 673, 701, 1064, 1127, 1476, 1601
TspGWI ACGGA 1 cut(s) 867
TspRI CASTG 3 cut(s) 112, 241, 386
VpaK11BI GGWCC 1 cut(s) 345
XapI RAATTY 4 cut(s) 481, 645, 680, 1479
XceI RCATGY 3 cut(s) 16, 318, 1783
XmiI GTMKAC 1 cut(s) 1365
XmnI GAANNNNTTC 1 cut(s) 243
ZrmI AGTACT 2 cut(s) 704, 1679
Zsp2I ATGCAT 2 cut(s) 14, 316
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.