Rroxscaffold_1G00060060

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000066
Physical Location & Seq
Forward (+)
82199961 .. 82202764
2804 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00060060.1

Sequence Viewer

Length: 2160 bp
ATGTCGATCATGGCCTTGCAATTCCCATTGGCGGCAGTCCTAATCCTATTATCGGCGGCTAGTACTACGACAACAGCAGCTGCTGCTCAAGCCCTGCCTGGCTGCCCTGACAAGTGCGGTAATCTCACAATTCCATATCCATTTGGCATGAAGAAGGATTGTTACCTGGTAGATAGGTTCTACATCAAATGTAGCCAGGGGGCCCAACTGGACCAACCCCCAAAAGCCTACTTTGGGGACACCGATATCATTGTTACTAACATATCCATAGACGTTGGGGAGTTGCGAATGTTGAGCCATAGAGGTTTAGATTGTTACAATAAAGAGGGCAATCGAACTTCCAGAACCAGACCTGAGCTTCCGAGGGGGAAGAAGAAATCGTTACTGGGTGCATGTCAGTATGCAACAGCCTTGGCGTTGTTGATGACAGCTCTTGCTCTGGCGTTGGGTGTTGCCAAACCAACATCCCCAGTGGACTGCACAATCGTACTCTACGCCTTCATTGTAGAGGAAGGCCAGTTCAATTTCTCCTCTTCCAGTTTTAAAGAACTGAATGGCACTAAACGGCTTCCAATGGTCCTTAATTGGGCCATTGGGAATGGCACAGACCCCTGTCATGAAGCTCAGAAAAGGAAGGATTACGCATGCAGAGCTAAAAGCATATGCAAAAACAGGCCTATCAATGGGTCTGTAGGTTATTTGTGCGAGTGTTTGCCTGGTTATGAAGGAAACCCGTACCACCCAGATGGTTGCAAAGGTGATATTGACGAGTGCAAGGCTCCAAACCTCTGCCAGTACGGACAGTGCAAAAATATTCCACCTCCAGGAAATTACTCTTGTTCATGTTACAAAGGATATAAAAACGACGGCTTGAATGACAAGATTTGCATCAAAGACGATTCCAAAAGATACTCAAAGATCGTTCTCCTGCTGATGGTTTCATTGGGTGTAAGTGTGGGCTTCTTGGTTTTATTCCTTGGAATTTTGTGGATATGTTGGGGAATGAAGAAAAGAGAGTTCATTAAACTCAAAGAAAAGTACTTCAAAGAGAATGGTGGCTTATTGTTACTGCAACAACTCGCTAGTCATGGAAGCTCGATGAAGACAACAAAAGTCTTTACTGCTGAAGAACTTGAGAAGGCAACAAACAACTATCACGAGAGTAGAGTCCTTGGAGAAGGAGGTTATGGAACAGTTTACAAAGGTATACTACCGGATGACACAATGGTTGCCATAAAGAAGTCGAAAGGTGGTGCCCTAACCCAGAGTGATCAGTTTGTTAACGAGGTGATTGTCCTTTCTCAAATCAACCACAGAAATGTGGTAAAGCTATTAGGTTGTTGTTTAGAAACAGAAGCGCCTTTACTAGTATACGAATTCATTACCCATGGTACTCTTTATGAGCATATTCATAAAAAAGGATCATCCCTCCCATTTGAATTACGAATGAAGATAGCAACTCAAAGCGCGGAAGCGCTGTCCCACTTACACTCCTCAATTTCCACACCAATAATACATCGAGATGTGAAAACATCAAATATTTTGTTGGATGATGATTATACGGCAAAAGTCTCAGATTTTGGAGCTTCGCGATTGGTTCCTTCAGGTCAAACTGATATACAAACTTTAGTGCTTGGGACATTTGGATACTTGGACCCTGAATATCTGCAATCAAACCAACTAACAGAAAAGAGTGATGTTTACAGCTTTGGAGTTGTCCTAGTGGAGCTACTAACAAGCAAAATGCCGGTTTCTAAAGATAGATGCTTAGCAAGCATCTTTCTTGCTTCCATGGAAGAAGGTTGGTTGAATCAAATTCTTGATGATGGCATAGTGAATGAGGGAAACATTGAGACGGTAAACAGAGTGGCAAATCTCGCAAAACGATGCTTGAGGGTAAACGGGGAGGAAAGGCCTACCATGAAAGAAGTTGCGAAAGAGTTAGAGGAAATAAGTGTCATGGAAAAACATCCGTGGGGATTTGATACTAATTTTTGCGAAGAAGAGACTGAATACTTGCTTGGGTCAGTTAATTCAGACGCTTATGTTGTGGGTAATGGAGAAGGTGATTGCAGTTCTAGTGGTCTAACCGGTGGTACGACCACTGCGTTTGACAGCATGCAAATCGAACAGTTAATGCCACATGCTGGTGGACGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK

Protein Analysis

719

Amino Acids

78.96

Weight (kDa)

6.21

Isoelectric Point (pI)

35.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 35 - 96 1e-07 Wall-associated receptor kinase galacturonan-binding
Pkinase PF00069 385 - 646 1.1e-49 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 388 - 648 1.1e-50 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21210 AT1G21230 AT1G21240 AT1G21240 AT1G21245 AT1G21250 AT1G21270 AT1G22720 AT3G25490
fragaria_vesca FvH4_2g35051 FvH4_2g35051 FvH4_2g35060 FvH4_3g10281 FvH4_3g10285 FvH4_3g10286 FvH4_3g10311 FvH4_3g10311 FvH4_3g10320 FvH4_3g10332 FvH4_3g10340 FvH4_3g10350 FvH4_3g10360 FvH4_3g10390 FvH4_3g10412 FvH4_3g10413 FvH4_3g10461 FvH4_3g10462 FvH4_3g10470 FvH4_3g10510 FvH4_3g10520 FvH4_3g10530 FvH4_3g10550 FvH4_3g10560 FvH4_3g10611 FvH4_3g11730 FvH4_6g53140 FvH4_6g53142 FvH4_7g31880 FvH4_7g31910 FvH4_7g31920
malus_domestica MD05G1269900.v1.1 MD05G1270000.v1.1 MD05G1270200.v1.1 MD05G1270400.v1.1 MD05G1270500.v1.1 MD05G1270600.v1.1 MD05G1270800.v1.1 MD05G1270900.v1.1 MD07G1183800.v1.1 MD08G1107600.v1.1 MD10G1248500.v1.1 MD10G1249200.v1.1 MD10G1249500.v1.1 MD10G1250000.v1.1 MD10G1250500.v1.1 MD10G1250900.v1.1 MD10G1251200.v1.1 MD10G1251400.v1.1 MD13G1004600.v1.1 MD15G1088400.v1.1
prunus_persica Prupe.1G442400_v2.0.a1 Prupe.1G442500_v2.0.a1 Prupe.2G223400_v2.0.a1 Prupe.4G091900_v2.0.a1 Prupe.4G093200_v2.0.a1 Prupe.4G093300_v2.0.a1 Prupe.4G093400_v2.0.a1 Prupe.4G093500_v2.0.a1 Prupe.4G094100_v2.0.a1 Prupe.4G094200_v2.0.a1 Prupe.4G094300_v2.0.a1 Prupe.6G358300_v2.0.a1
pyrus_communis pycom08g08990 pycom10g20660 pycom10g20670 pycom10g20680 pycom10g20710 pycom10g20740 pycom13g00400 pycom15g08290
rosa_chinensis RchiOBHm_Chr1g0326861 RchiOBHm_Chr1g0326871 RchiOBHm_Chr1g0331391 RchiOBHm_Chr1g0336911 RchiOBHm_Chr1g0364001 RchiOBHm_Chr2g0174541 RchiOBHm_Chr2g0175041 RchiOBHm_Chr2g0175101 RchiOBHm_Chr2g0175121 RchiOBHm_Chr3g0486331 RchiOBHm_Chr5g0015581 RchiOBHm_Chr5g0016551 RchiOBHm_Chr5g0016561 RchiOBHm_Chr5g0016581 RchiOBHm_Chr5g0016601 RchiOBHm_Chr5g0016611 RchiOBHm_Chr5g0016641 RchiOBHm_Chr5g0016661 RchiOBHm_Chr5g0016691 RchiOBHm_Chr5g0016701 RchiOBHm_Chr5g0016731 RchiOBHm_Chr5g0016741 RchiOBHm_Chr5g0016811 RchiOBHm_Chr5g0016841 RchiOBHm_Chr5g0016871 RchiOBHm_Chr5g0016881 RchiOBHm_Chr5g0016931 RchiOBHm_Chr5g0017021 RchiOBHm_Chr5g0017041 RchiOBHm_Chr5g0017071 RchiOBHm_Chr5g0017091 RchiOBHm_Chr5g0017121 RchiOBHm_Chr5g0017141 RchiOBHm_Chr5g0017291 RchiOBHm_Chr5g0017321 RchiOBHm_Chr5g0017401 RchiOBHm_Chr5g0019001 RchiOBHm_Chr5g0019071 RchiOBHm_Chr5g0028941 RchiOBHm_Chr5g0052141 RchiOBHm_Chr5g0054721 RchiOBHm_Chr5g0054801 RchiOBHm_Chr5g0054931 RchiOBHm_Chr5g0054941 RchiOBHm_Chr6g0259291 RchiOBHm_Chr6g0292991 RchiOBHm_Chr6g0306911
rosa_laevigata RLG00000009465 RLG00000009468 RLG00000010755 RLG00000011976 RLG00000014541 RLG00000014542 RLG00000015169 RLG00000022308 RLG00000027529 RLG00000029324 RLG00000030244 RLG00000030245 RLG00000032210 RLG00000032211 RLG00000032257 RLG00000032259 RLG00000032260 RLG00000032261 RLG00000032262 RLG00000032268 RLG00000032269 RLG00000032272 RLG00000032282 RLG00000032283 RLG00000032308 RLG00000032413 RLG00000033129 RLG00000034945
rosa_multiflora Rmu_co8241929.1_g000001 Rmu_co8269227.1_g000001 Rmu_co8461259.1_g000001 Rmu_co8495385.1_g000001 Rmu_sc0000028.1_g000003 Rmu_sc0000083.1_g000009 Rmu_sc0000083.1_g000010 Rmu_sc0000083.1_g000031 Rmu_sc0000083.1_g000043 Rmu_sc0000083.1_g000046 Rmu_sc0000083.1_g000047 Rmu_sc0000563.1_g000024 Rmu_sc0000842.1_g000012 Rmu_sc0001235.1_g000001 Rmu_sc0001235.1_g000005 Rmu_sc0001235.1_g000009 Rmu_sc0001235.1_g000015 Rmu_sc0001235.1_g000016 Rmu_sc0001235.1_g000017 Rmu_sc0001473.1_g000036 Rmu_sc0001607.1_g000016 Rmu_sc0001788.1_g000006 Rmu_sc0002018.1_g000008 Rmu_sc0002028.1_g000001 Rmu_sc0002680.1_g000011 Rmu_sc0002798.1_g000023 Rmu_sc0002886.1_g000023 Rmu_sc0002989.1_g000012 Rmu_sc0003064.1_g000009 Rmu_sc0003382.1_g000006 Rmu_sc0003630.1_g000002 Rmu_sc0003630.1_g000047 Rmu_sc0003630.1_g000066 Rmu_sc0003630.1_g000067 Rmu_sc0003649.1_g000010 Rmu_sc0003688.1_g000005 Rmu_sc0003920.1_g000007 Rmu_sc0004765.1_g000007 Rmu_sc0004765.1_g000008 Rmu_sc0004920.1_g000011 Rmu_sc0005223.1_g000008 Rmu_sc0005223.1_g000010 Rmu_sc0005223.1_g000015 Rmu_sc0005223.1_g000016 Rmu_sc0005319.1_g000014 Rmu_sc0005319.1_g000015 Rmu_sc0005319.1_g000018 Rmu_sc0005319.1_g000019 Rmu_sc0005319.1_g000025 Rmu_sc0005418.1_g000007 Rmu_sc0005418.1_g000012 Rmu_sc0006106.1_g000011 Rmu_sc0006914.1_g000012 Rmu_sc0007023.1_g000001 Rmu_sc0007662.1_g000001 Rmu_sc0007920.1_g000002 Rmu_sc0007920.1_g000013 Rmu_sc0008053.1_g000016 Rmu_sc0008053.1_g000017 Rmu_sc0008053.1_g000018 Rmu_sc0008053.1_g000023 Rmu_sc0008053.1_g000050 Rmu_sc0009042.1_g000007 Rmu_sc0009042.1_g000008 Rmu_sc0010642.1_g000008 Rmu_sc0011109.1_g000002 Rmu_sc0011314.1_g000006 Rmu_sc0011974.1_g000006 Rmu_sc0012179.1_g000009 Rmu_sc0015169.1_g000001 Rmu_sc0016368.1_g000002 Rmu_sc0022659.1_g000001 Rmu_sc0027371.1_g000001 Rmu_sc0028213.1_g000001 Rmu_sc0034526.1_g000001 Rmu_sc0039366.1_g000002 Rmu_ssc0000050.1_g000084 Rmu_ssc0000050.1_g000088 Rmu_ssc0000167.1_g000017
rosa_roxburghii Rroxscaffold_1G00011340 Rroxscaffold_1G00025710 Rroxscaffold_1G00025870 Rroxscaffold_1G00026070 Rroxscaffold_1G00026170 Rroxscaffold_1G00026200 Rroxscaffold_1G00026270 Rroxscaffold_1G00050950 Rroxscaffold_1G00058900 Rroxscaffold_1G00060040 Rroxscaffold_1G00060060 Rroxscaffold_1G00060070 Rroxscaffold_1G00060080 Rroxscaffold_1G00060110 Rroxscaffold_1G00060120 Rroxscaffold_1G00060140 Rroxscaffold_1G00060170 Rroxscaffold_1G00060210 Rroxscaffold_1G00060220 Rroxscaffold_1G00060240 Rroxscaffold_1G00060250 Rroxscaffold_1G00060260 Rroxscaffold_1G00060300 Rroxscaffold_1G00060320 Rroxscaffold_1G00060330 Rroxscaffold_1G00060900 Rroxscaffold_1G00060920 Rroxscaffold_1G00073980 Rroxscaffold_2G00077560 Rroxscaffold_2G00077580 Rroxscaffold_2G00146990 Rroxscaffold_3G00236200 Rroxscaffold_3G00236210 Rroxscaffold_3G00236220 Rroxscaffold_4G00292760 Rroxscaffold_4G00315200 Rroxscaffold_4G00315240 Rroxscaffold_4G00326240 Rroxscaffold_4G00328980 Rroxscaffold_5G00341890 Rroxscaffold_7G00161350 Rroxscaffold_7G00174490 Rroxscaffold_7G00206550
rosa_rugosa Rorug01G0093000 Rorug01G0093100 Rorug01G0128800 Rorug01G0312000 Rorug01G0312000 Rorug02G0581100 Rorug03G0224600 Rorug05G0028500 Rorug05G0032700 Rorug05G0032800 Rorug05G0032900 Rorug05G0032900 Rorug05G0033000 Rorug05G0033100 Rorug05G0033300 Rorug05G0033400 Rorug05G0033900 Rorug05G0034900 Rorug05G0035000 Rorug05G0046900 Rorug05G0047500 Rorug05G0047500 Rorug05G0111600 Rorug05G0287900 Rorug05G0288700 Rorug05G0586900 Rorug06G0231700 Rorug06G0354300 Rorug06G0354400
rosa_samantha Rh1DG122200 Rh2DG686800 Rh5BG136600 Rh6AG344500 Rh6BG351900 Rh6CG359000 Rh6CG359100 Rh6CG387600 Rh6CG480500 Rh6DG344600 Rh6DG467400
rosa_wichuraiana Rw0G010250 Rw0G014820 Rw0G021400 Rw0G023100 Rw1G009450 Rw1G012100 Rw1G012180 Rw1G028360 Rw2G054260 Rw3G024370 Rw3G024420 Rw3G024470 Rw5G010850 Rw5G011010 Rw5G011020 Rw5G011050 Rw5G011080 Rw5G011120 Rw5G012300 Rw5G018470 Rw5G033830 Rw6G008930 Rw6G030070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1253
AccB7I CCANNNNNTGG 1 cut(s) 2147
AccI GTMKAC 2 cut(s) 1207, 1371
AccII CGCG 2 cut(s) 1469, 1591
AciI CCGC 4 cut(s) 32, 56, 117, 1469
AclWI GGATC 1 cut(s) 1429
AcsI RAATTY 3 cut(s) 981, 1376, 1815
AcuI CTGAAG 2 cut(s) 1146, 1587
AfaI GTAC 7 cut(s) 64, 489, 737, 797, 1040, 1393, 2098
AfeI AGCGCT 1 cut(s) 1476
AfiI CCNNNNNNNGG 8 cut(s) 31, 52, 234, 586, 683, 824, 934, 2147
AgeI ACCGGT 1 cut(s) 2090
AgsI TTSAA 5 cut(s) 523, 874, 1045, 1439, 1810
AhlI ACTAGT 1 cut(s) 1366
AjnI CCWGG 5 cut(s) 97, 165, 195, 715, 823
AjuI GAANNNNNNNTTGG 2 cut(s) 2004, 2036
AloI GAACNNNNNNTCC 2 cut(s) 503, 535
Alw26I GTCTC 3 cut(s) 1576, 1847, 2000
AlwI GGATC 1 cut(s) 1429
AlwNI CAGNNNCTG 2 cut(s) 80, 83
Aor51HI AGCGCT 1 cut(s) 1476
AoxI GGCC 6 cut(s) 12, 201, 514, 588, 674, 1913
ApaI GGGCCC 1 cut(s) 205
ApeKI GCWGC 4 cut(s) 77, 80, 83, 102
ApoI RAATTY 3 cut(s) 981, 1376, 1815
AsiGI ACCGGT 1 cut(s) 2090
AspLEI GCGC 3 cut(s) 1360, 1469, 1477
AspS9I GGNCC 6 cut(s) 201, 202, 211, 577, 588, 1654
AsuHPI GGTGA 3 cut(s) 770, 1300, 2078
AvaII GGWCC 3 cut(s) 211, 577, 1654
BaeGI GKGCMC 2 cut(s) 205, 1258
BanI GGYRCC 1 cut(s) 1253
BanII GRGCYC 1 cut(s) 205
BarI GAAGNNNNNNTAC 4 cut(s) 146, 178, 1347, 1379
BauI CACGAG 1 cut(s) 1157
BbsI GAAGAC 1 cut(s) 1109
BbvI GCAGC 4 cut(s) 67, 70, 89, 89
BccI CCATC 3 cut(s) 740, 928, 1820
BceAI ACGGC 3 cut(s) 581, 883, 1578
BcgI CGANNNNNNTGC 2 cut(s) 2107, 2141
BciT130I CCWGG 5 cut(s) 99, 167, 197, 717, 825
BciVI GTATCC 1 cut(s) 1640
BclI TGATCA 1 cut(s) 1270
BcoDI GTCTC 3 cut(s) 1576, 1847, 2000
BcuI ACTAGT 1 cut(s) 1366
BfaI CTAG 5 cut(s) 60, 1083, 1367, 1721, 2079
BfmI CTRYAG 1 cut(s) 690
BfoI RGCGCY 2 cut(s) 1361, 1478
BfuI GTATCC 1 cut(s) 1640
BisI GCNGC 6 cut(s) 33, 57, 78, 81, 84, 103
BlpI GCTNAGC 1 cut(s) 1768
BlsI GCNGC 6 cut(s) 34, 58, 79, 82, 85, 104
BmcAI AGTACT 2 cut(s) 64, 1040
Bme1390I CCNGG 5 cut(s) 99, 167, 197, 717, 825
Bme18I GGWCC 3 cut(s) 211, 577, 1654
BmgT120I GGNCC 6 cut(s) 201, 202, 211, 577, 588, 1654
BmiI GGNNCC 6 cut(s) 202, 203, 780, 1255, 1599, 1656
BmrFI CCNGG 5 cut(s) 99, 167, 197, 717, 825
BmrI ACTGGG 2 cut(s) 395, 464
BmsI GCATC 4 cut(s) 897, 1754, 1785, 1877
BmuI ACTGGG 2 cut(s) 395, 464
BoxI GACNNNNGTC 1 cut(s) 612
BpiI GAAGAC 1 cut(s) 1109
BpmI CTGGAG 1 cut(s) 807
Bpu10I CCTNAGC 1 cut(s) 354
Bpu1102I GCTNAGC 1 cut(s) 1768
BpuEI CTTGAG 3 cut(s) 72, 1154, 1912
BsaBI GATNNNNATC 1 cut(s) 887
BsaJI CCNNGG 8 cut(s) 196, 362, 411, 976, 1171, 1387, 1791, 1973
BsaWI WCCGGW 2 cut(s) 1213, 2090
BsaXI ACNNNNNCTCC 2 cut(s) 1475, 1505
Bsc4I CCNNNNNNNGG 8 cut(s) 31, 52, 234, 586, 683, 824, 934, 2147
Bse118I RCCGGY 2 cut(s) 1747, 2090
Bse1I ACTGG 6 cut(s) 213, 390, 470, 517, 537, 793
Bse8I GATNNNNATC 1 cut(s) 887
BseBI CCWGG 5 cut(s) 99, 167, 197, 717, 825
BseDI CCNNGG 8 cut(s) 196, 362, 411, 976, 1171, 1387, 1791, 1973
BseGI GGATG 5 cut(s) 464, 1222, 1424, 1555, 1969
BseJI GATNNNNATC 1 cut(s) 887
BseLI CCNNNNNNNGG 8 cut(s) 31, 52, 234, 586, 683, 824, 934, 2147
BseMII CTCAG 3 cut(s) 345, 638, 1587
BseNI ACTGG 6 cut(s) 213, 390, 470, 517, 537, 793
BseRI GAGGAG 2 cut(s) 520, 1483
BseSI GKGCMC 2 cut(s) 205, 1258
BseXI GCAGC 4 cut(s) 67, 70, 89, 89
BsgI GTGCAG 1 cut(s) 463
Bsh1236I CGCG 2 cut(s) 1469, 1591
BshFI GGCC 6 cut(s) 14, 203, 516, 590, 676, 1915
BshNI GGYRCC 1 cut(s) 1253
BshTI ACCGGT 1 cut(s) 2090
BsiSI CCGG 3 cut(s) 1214, 1748, 2091
BslFI GGGAC 3 cut(s) 251, 1465, 1651
BslI CCNNNNNNNGG 8 cut(s) 31, 52, 234, 586, 683, 824, 934, 2147
BsmAI GTCTC 3 cut(s) 1576, 1847, 2000
BsmBI CGTCTC 1 cut(s) 1847
BsmFI GGGAC 3 cut(s) 251, 1465, 1651
BsnI GGCC 6 cut(s) 14, 203, 516, 590, 676, 1915
Bsp120I GGGCCC 1 cut(s) 201
Bsp1286I GDGCHC 2 cut(s) 205, 1258
Bsp143I GATC 4 cut(s) 6, 918, 1270, 1421
Bsp1720I GCTNAGC 1 cut(s) 1768
Bsp19I CCATGG 2 cut(s) 1387, 1791
Bsp68I TCGCGA 1 cut(s) 1591
BspACI CCGC 4 cut(s) 32, 56, 117, 1469
BspANI GGCC 6 cut(s) 14, 203, 516, 590, 676, 1915
BspCNI CTCAG 3 cut(s) 346, 637, 1586
BspFNI CGCG 2 cut(s) 1469, 1591
BspHI TCATGA 1 cut(s) 616
BspLI GGNNCC 6 cut(s) 202, 203, 780, 1255, 1599, 1656
BspPI GGATC 1 cut(s) 1429
BspT107I GGYRCC 1 cut(s) 1253
BsrFI RCCGGY 2 cut(s) 1747, 2090
BsrI ACTGG 6 cut(s) 213, 390, 470, 517, 537, 793
BssAI RCCGGY 2 cut(s) 1747, 2090
BssECI CCNNGG 8 cut(s) 196, 362, 411, 976, 1171, 1387, 1791, 1973
BssMI GATC 4 cut(s) 6, 918, 1270, 1421
BssNAI GTATAC 2 cut(s) 1208, 1372
BssSI CACGAG 1 cut(s) 1157
BssT1I CCWWGG 5 cut(s) 411, 976, 1171, 1387, 1791
Bst1107I GTATAC 2 cut(s) 1208, 1372
Bst2BI CACGAG 1 cut(s) 1157
Bst2UI CCWGG 5 cut(s) 99, 167, 197, 717, 825
Bst4CI ACNGT 4 cut(s) 804, 1195, 1858, 2133
Bst6I CTCTTC 2 cut(s) 538, 1998
BstAPI GCANNNNNTGC 1 cut(s) 83
BstC8I GCNNGC 3 cut(s) 646, 1774, 2120
BstDEI CTNAG 4 cut(s) 354, 624, 1573, 1768
BstDSI CCRYGG 3 cut(s) 1387, 1791, 1973
BstF5I GGATG 5 cut(s) 464, 1222, 1424, 1555, 1969
BstFNI CGCG 2 cut(s) 1469, 1591
BstH2I RGCGCY 2 cut(s) 1361, 1478
BstHHI GCGC 3 cut(s) 1360, 1469, 1477
BstKTI GATC 4 cut(s) 9, 921, 1273, 1424
BstMAI GTCTC 3 cut(s) 1576, 1847, 2000
BstMBI GATC 4 cut(s) 6, 918, 1270, 1421
BstMWI GCNNNNNNNGC 5 cut(s) 83, 89, 650, 1773, 1877
BstNI CCWGG 5 cut(s) 99, 167, 197, 717, 825
BstNSI RCATGY 4 cut(s) 396, 648, 2122, 2147
BstPAI GACNNNNGTC 1 cut(s) 612
BstSCI CCNGG 5 cut(s) 97, 165, 195, 715, 823
BstSFI CTRYAG 1 cut(s) 690
BstSLI GKGCMC 2 cut(s) 205, 1258
BstUI CGCG 2 cut(s) 1469, 1591
BstV1I GCAGC 4 cut(s) 67, 70, 89, 89
BstV2I GAAGAC 1 cut(s) 1109
BstXI CCANNNNNNTGG 1 cut(s) 746
BstZ17I GTATAC 2 cut(s) 1208, 1372
BsuI GTATCC 1 cut(s) 1640
BsuRI GGCC 6 cut(s) 14, 203, 516, 590, 676, 1915
BtgI CCRYGG 3 cut(s) 1387, 1791, 1973
BtsCI GGATG 5 cut(s) 464, 1222, 1424, 1555, 1969
BtsI GCAGTG 1 cut(s) 2103
BtsIMutI CAGTG 3 cut(s) 477, 809, 2103
BtuMI TCGCGA 1 cut(s) 1591
Cac8I GCNNGC 3 cut(s) 646, 1774, 2120
CaiI CAGNNNCTG 2 cut(s) 80, 83
CciI TCATGA 1 cut(s) 616
CfoI GCGC 3 cut(s) 1360, 1469, 1477
Cfr10I RCCGGY 2 cut(s) 1747, 2090
Cfr13I GGNCC 6 cut(s) 201, 202, 211, 577, 588, 1654
CseI GACGC 1 cut(s) 2048
CsiI ACCWGGT 1 cut(s) 165
Csp6I GTAC 7 cut(s) 63, 488, 736, 796, 1039, 1392, 2097
CspAI ACCGGT 1 cut(s) 2090
CspCI CAANNNNNGTGG 2 cut(s) 2092, 2127
CviQI GTAC 7 cut(s) 63, 488, 736, 796, 1039, 1392, 2097
DdeI CTNAG 4 cut(s) 354, 624, 1573, 1768
DpnI GATC 4 cut(s) 8, 920, 1272, 1423
DpnII GATC 4 cut(s) 6, 918, 1270, 1421
DraI TTTAAA 1 cut(s) 544
Eam1104I CTCTTC 2 cut(s) 538, 1998
EarI CTCTTC 2 cut(s) 538, 1998
Eco130I CCWWGG 5 cut(s) 411, 976, 1171, 1387, 1791
Eco147I AGGCCT 2 cut(s) 676, 1915
Eco24I GRGCYC 1 cut(s) 205
Eco32I GATATC 1 cut(s) 247
Eco47I GGWCC 3 cut(s) 211, 577, 1654
Eco47III AGCGCT 1 cut(s) 1476
Eco57I CTGAAG 2 cut(s) 1146, 1587
EcoO109I RGGNCCY 1 cut(s) 201
EcoRI GAATTC 1 cut(s) 1376
EcoRII CCWGG 5 cut(s) 97, 165, 195, 715, 823
EcoRV GATATC 1 cut(s) 247
EcoT14I CCWWGG 5 cut(s) 411, 976, 1171, 1387, 1791
EcoT38I GRGCYC 1 cut(s) 205
ErhI CCWWGG 5 cut(s) 411, 976, 1171, 1387, 1791
Esp3I CGTCTC 1 cut(s) 1847
FaqI GGGAC 3 cut(s) 251, 1465, 1651
FauNDI CATATG 1 cut(s) 662
FbaI TGATCA 1 cut(s) 1270
FblI GTMKAC 2 cut(s) 1207, 1371
Fnu4HI GCNGC 6 cut(s) 33, 57, 78, 81, 84, 103
FokI GGATG 5 cut(s) 451, 1229, 1411, 1562, 1956
FriOI GRGCYC 1 cut(s) 205
Fsp4HI GCNGC 6 cut(s) 33, 57, 78, 81, 84, 103
FspBI CTAG 5 cut(s) 60, 1083, 1367, 1721, 2079
GlaI GCGC 3 cut(s) 1359, 1468, 1476
GluI GCNGC 6 cut(s) 33, 57, 78, 81, 84, 103
GsuI CTGGAG 1 cut(s) 807
HaeII RGCGCY 2 cut(s) 1361, 1478
HaeIII GGCC 6 cut(s) 14, 203, 516, 590, 676, 1915
HapII CCGG 3 cut(s) 1214, 1748, 2091
HgaI GACGC 1 cut(s) 2048
HhaI GCGC 3 cut(s) 1360, 1469, 1477
Hin6I GCGC 3 cut(s) 1358, 1467, 1475
HinP1I GCGC 3 cut(s) 1358, 1467, 1475
HincII GTYRAC 1 cut(s) 1282
HindII GTYRAC 1 cut(s) 1282
HinfI GANTC 3 cut(s) 899, 1167, 1810
HpaI GTTAAC 1 cut(s) 1282
HpaII CCGG 3 cut(s) 1214, 1748, 2091
HphI GGTGA 3 cut(s) 770, 1300, 2078
Hpy166II GTNNAC 9 cut(s) 475, 1198, 1208, 1282, 1372, 1702, 1861, 1900, 2153
Hpy188I TCNGA 4 cut(s) 363, 627, 1576, 2038
Hpy188III TCNNGA 6 cut(s) 342, 617, 1157, 1520, 1590, 1820
Hpy8I GTNNAC 9 cut(s) 475, 1198, 1208, 1282, 1372, 1702, 1861, 1900, 2153
Hpy99I CGWCG 1 cut(s) 869
HpyCH4III ACNGT 4 cut(s) 804, 1195, 1858, 2133
HpyCH4IV ACGT 1 cut(s) 273
HpyF10VI GCNNNNNNNGC 5 cut(s) 83, 89, 650, 1773, 1877
HpyF3I CTNAG 4 cut(s) 354, 624, 1573, 1768
HpySE526I ACGT 1 cut(s) 273
HspAI GCGC 3 cut(s) 1358, 1467, 1475
Ksp22I TGATCA 1 cut(s) 1270
KspAI GTTAAC 1 cut(s) 1282
Kzo9I GATC 4 cut(s) 6, 918, 1270, 1421
LmnI GCTCC 3 cut(s) 784, 1583, 1726
Lsp1109I GCAGC 4 cut(s) 67, 70, 89, 89
LweI GCATC 4 cut(s) 897, 1754, 1785, 1877
MabI ACCWGGT 1 cut(s) 165
MaeI CTAG 5 cut(s) 60, 1083, 1367, 1721, 2079
MaeII ACGT 1 cut(s) 273
MaeIII GTNAC 6 cut(s) 161, 253, 314, 381, 845, 1065
MalI GATC 4 cut(s) 8, 920, 1272, 1423
MboI GATC 4 cut(s) 6, 918, 1270, 1421
MhlI GDGCHC 2 cut(s) 205, 1258
MlyI GAGTC 1 cut(s) 1176
MmeI TCCRAC 1 cut(s) 1527
MseI TTAA 6 cut(s) 543, 582, 1023, 1281, 2031, 2135
MslI CAYNNNNRTG 4 cut(s) 744, 1317, 1521, 2148
MspA1I CMGCKG 1 cut(s) 80
MspI CCGG 3 cut(s) 1214, 1748, 2091
MspR9I CCNGG 5 cut(s) 99, 167, 197, 717, 825
MvaI CCWGG 5 cut(s) 99, 167, 197, 717, 825
MvnI CGCG 2 cut(s) 1469, 1591
MwoI GCNNNNNNNGC 5 cut(s) 83, 89, 650, 1773, 1877
NcoI CCATGG 2 cut(s) 1387, 1791
NdeI CATATG 1 cut(s) 662
NdeII GATC 4 cut(s) 6, 918, 1270, 1421
NlaIV GGNNCC 6 cut(s) 202, 203, 780, 1255, 1599, 1656
NruI TCGCGA 1 cut(s) 1591
NspI RCATGY 4 cut(s) 396, 648, 2122, 2147
PaeI GCATGC 2 cut(s) 648, 2122
PagI TCATGA 1 cut(s) 616
PceI AGGCCT 2 cut(s) 676, 1915
PcsI WCGNNNNNNNCGW 1 cut(s) 2105
PfeI GAWTC 2 cut(s) 899, 1810
PflMI CCANNNNNTGG 1 cut(s) 2147
PfoI TCCNGGA 1 cut(s) 823
PinAI ACCGGT 1 cut(s) 2090
PkrI GCNGC 6 cut(s) 34, 58, 79, 82, 85, 104
PleI GAGTC 1 cut(s) 1175
PpsI GAGTC 1 cut(s) 1175
PshAI GACNNNNGTC 1 cut(s) 612
Psp6I CCWGG 5 cut(s) 97, 165, 195, 715, 823
PspGI CCWGG 5 cut(s) 97, 165, 195, 715, 823
PspN4I GGNNCC 6 cut(s) 202, 203, 780, 1255, 1599, 1656
PspOMI GGGCCC 1 cut(s) 201
PspPI GGNCC 6 cut(s) 201, 202, 211, 577, 588, 1654
PstNI CAGNNNCTG 2 cut(s) 80, 83
PvuII CAGCTG 1 cut(s) 80
RruI TCGCGA 1 cut(s) 1591
RsaI GTAC 7 cut(s) 64, 489, 737, 797, 1040, 1393, 2098
RsaNI GTAC 7 cut(s) 63, 488, 736, 796, 1039, 1392, 2097
RseI CAYNNNNRTG 4 cut(s) 744, 1317, 1521, 2148
SaqAI TTAA 6 cut(s) 543, 582, 1023, 1281, 2031, 2135
SatI GCNGC 6 cut(s) 33, 57, 78, 81, 84, 103
Sau3AI GATC 4 cut(s) 6, 918, 1270, 1421
Sau96I GGNCC 6 cut(s) 201, 202, 211, 577, 588, 1654
ScaI AGTACT 2 cut(s) 64, 1040
SchI GAGTC 1 cut(s) 1176
ScrFI CCNGG 5 cut(s) 99, 167, 197, 717, 825
SduI GDGCHC 2 cut(s) 205, 1258
SexAI ACCWGGT 1 cut(s) 165
SfaNI GCATC 4 cut(s) 897, 1754, 1785, 1877
SfcI CTRYAG 1 cut(s) 690
SinI GGWCC 3 cut(s) 211, 577, 1654
SmiMI CAYNNNNRTG 4 cut(s) 744, 1317, 1521, 2148
SmlI CTYRAG 3 cut(s) 87, 1133, 1891
SmoI CTYRAG 3 cut(s) 87, 1133, 1891
SpeI ACTAGT 1 cut(s) 1366
SphI GCATGC 2 cut(s) 648, 2122
SseBI AGGCCT 2 cut(s) 676, 1915
SsiI CCGC 4 cut(s) 32, 56, 117, 1469
SspI AATATT 2 cut(s) 814, 1540
SspMI CTAG 5 cut(s) 60, 1083, 1367, 1721, 2079
StuI AGGCCT 2 cut(s) 676, 1915
StyD4I CCNGG 5 cut(s) 97, 165, 195, 715, 823
StyI CCWWGG 5 cut(s) 411, 976, 1171, 1387, 1791
TaaI ACNGT 4 cut(s) 804, 1195, 1858, 2133
TaiI ACGT 1 cut(s) 276
TaqI TCGA 6 cut(s) 5, 334, 1097, 1244, 1519, 2127
TatI WGTACW 2 cut(s) 62, 1038
TauI GCSGC 2 cut(s) 35, 59
TfiI GAWTC 2 cut(s) 899, 1810
Tru1I TTAA 6 cut(s) 543, 582, 1023, 1281, 2031, 2135
Tru9I TTAA 6 cut(s) 543, 582, 1023, 1281, 2031, 2135
TscAI CASTG 3 cut(s) 477, 809, 2110
TseI GCWGC 4 cut(s) 77, 80, 83, 102
TspGWI ACGGA 2 cut(s) 813, 1962
TspRI CASTG 3 cut(s) 477, 809, 2110
Van91I CCANNNNNTGG 1 cut(s) 2147
VpaK11BI GGWCC 3 cut(s) 211, 577, 1654
XapI RAATTY 3 cut(s) 981, 1376, 1815
XceI RCATGY 4 cut(s) 396, 648, 2122, 2147
XmiI GTMKAC 2 cut(s) 1207, 1371
XspI CTAG 5 cut(s) 60, 1083, 1367, 1721, 2079
ZrmI AGTACT 2 cut(s) 64, 1040
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.