Rroxscaffold_3G00236210

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000066
Physical Location & Seq
Reverse (-)
23381279 .. 23385094
3816 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00236210.1

Sequence Viewer

Length: 2115 bp
ATGGCGGCCTTGCAGGGTATCTTGCTCATCATCCAATTCTCATTCGTAATCATCGGGGTACTGATTGTATCAACAACAGCAGCAGCACCTCAAGCGCTGCCTAACTGCCCTGACAAATGTGGTAATCTTACAGTTCCATACCCATTTGGCATGGCGGAAGGTTGTTACCGAGGAAATGCCTTCTTTGTCAATTGTAGTCAAGCAACCGATCCCCCAGTAGCATATTTGACCAAAAATGGCACTGTCATCATTACCAACATATCCCTTGCTGCGGGAGAGTTGCAAATATCAAATTTTATAGCTCGAGATTGCTATAGTTCCTCAGCTCTGAGAACCAGAAGGAACAGCCCTGTGCTACGGCTGCCTTCTCCTTACACCATCTCTGATACCAAAAACAAATTCATTGCCGTTGGTTGTGACACTTACGCAATTTTCAAAGGATTCCGGGGCACAGAACAGTCCATAACTGGGTGCATGTCAGTATGTAACAGCCTAGAAAGCGTTGATCAGAATTCTTGCTCCGGGGTTGGGTGTTGCCAAACAAACATTCCTAGCGGACTGCATAACCGTACTGTGACACTGGATAGCTATAGCAATCATTCAGGTATATGGGACTTTAACCCTTGCAGCTTTGCATTCATTGTTGAAGAGACACAGTTCAAGTTCTCCGGGAACACAAGTTTTCAACAACTGAACAGCACTACACGGCTTCCAATGGTTCTTAATTGGGCAATTGGGGATGAGCCAGACCCATGTGATGTAGCTCAAAAGAGGCAGGATTTCGTATGCAAGCAAAATAGCAAGTGTGTGAATCCCACAAATAGGAATGGTTACATTTGCCAGTGCTTGGAAGGTTATGAAGGGAACCCATACCACCCAGATGGGTGCCAAGATATTGATGAGTGCAAGGATCCAAATCTCTGCAACCACGGAGGGTGCAAAAATTTGGCGGGAAGTTACACTTGTTTGTGCTCAAAAGGATACAAGATCGACAGCTTGAATGACAAGAGTTGCATCAAAGAGAATCCCAAAAATAACTCAAAGATGACTCTCCTGCTTATTATTTCATTAAGTGTAAGTATAGGCTTATTGGTTTTATTTGTGGGGATTTCGTGGATATGTTGCGGGATAAAGAGAAGACAATACACAAAACTCAAAGAAAAGTACTTCAAAGAGAATGGTGGCTTATTGTTACTACAACAACTTGCTAGTCATGGAGGCACTGTGGAGACAACAAAAATCTTTTCCACAGAAGAACTTGAGAAGGCAACAAACAATTACCACGAGAGTAGAATCCTTGGAGAAGGAGGCTATGGAACAGTTTACAAAGGAATACTACCAGATGACAAAGTGGTGGCCATAAAGAAGTCAAAAGGTGGTGCCCCAACCCAAAGTGATCAGTTTGTTAACGAGATAGCAGTTGAAACGGCAGAAGCACTAGCATACTTACACTCCTCAACTTCAAATCCAATCATACATCGAGATGTGAAAGCAGAAAATATACTCTTAGATGATGCTTATACAGCAAAAGTATCAGACTTTGGAGCTTCAAGATTGATTCCATCAGGTCAAACTGAAATACAAACTTTAGTGCTAGGGACATTCGGTTACCTGGACCCTGAATATCTGCAATCGAACCAACTAACAGAAAAGAGTGACGTCTACAGCTTTGGTGTCGTCCTAGTGGAGCTACTAACAAGCAAAATGGCACTTTCTAAGGACAGATGCTTAACAAGCATCTTTATTTCTTCCATGGATGAAGATTGCTTGAATCAAATTCTTGATGATGACATTGTGAATGAGGGAAACATTGAGACAGTAAAGAATGTGGCCAGTCTTGCAAAAAGATGCTTGAGGGTAAAAGGGGAGGAAAGGCCTACCATGAAAGAAGTAGCAATGGAGCTAGAGGGAATGAGGATTACAGCAAAGCACCCATGGGGAGCTAATGTTGGTTTCTGTCCAGAGGAAAATGAGTACTTGCTTGGGTCACTTGATTCAGATGCTTATGTTTTGGATGTTAGAGGAGGTGGTGGTAGTTCTAGTGGTTTAACTACTGGTACAGGGTATGACAGCATGCAAATACAAATGCTAATGCCTTATGGTGATGGACGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK

Protein Analysis

704

Amino Acids

76.78

Weight (kDa)

5.31

Isoelectric Point (pI)

33.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 36 - 97 7.5e-10 Wall-associated receptor kinase galacturonan-binding
EGF_CA PF07645 298 - 338 1.4e-07 Calcium-binding EGF domain
Pkinase PF00069 467 - 631 1.3e-30 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 471 - 635 2.3e-25 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21210 AT1G21230 AT1G21240 AT1G21240 AT1G21245 AT1G21250 AT1G21270 AT1G22720 AT3G25490
fragaria_vesca FvH4_2g35051 FvH4_2g35051 FvH4_2g35060 FvH4_3g10281 FvH4_3g10285 FvH4_3g10286 FvH4_3g10311 FvH4_3g10311 FvH4_3g10320 FvH4_3g10332 FvH4_3g10340 FvH4_3g10350 FvH4_3g10360 FvH4_3g10390 FvH4_3g10412 FvH4_3g10413 FvH4_3g10461 FvH4_3g10462 FvH4_3g10470 FvH4_3g10510 FvH4_3g10520 FvH4_3g10530 FvH4_3g10550 FvH4_3g10560 FvH4_3g10611 FvH4_3g11730 FvH4_6g53140 FvH4_6g53142 FvH4_7g31880 FvH4_7g31910 FvH4_7g31920
malus_domestica MD05G1269900.v1.1 MD05G1270000.v1.1 MD05G1270200.v1.1 MD05G1270400.v1.1 MD05G1270500.v1.1 MD05G1270600.v1.1 MD05G1270800.v1.1 MD05G1270900.v1.1 MD07G1183800.v1.1 MD08G1107600.v1.1 MD10G1248500.v1.1 MD10G1249200.v1.1 MD10G1249500.v1.1 MD10G1250000.v1.1 MD10G1250500.v1.1 MD10G1250900.v1.1 MD10G1251200.v1.1 MD10G1251400.v1.1 MD13G1004600.v1.1 MD15G1088400.v1.1
prunus_persica Prupe.1G442400_v2.0.a1 Prupe.1G442500_v2.0.a1 Prupe.2G223400_v2.0.a1 Prupe.4G091900_v2.0.a1 Prupe.4G093200_v2.0.a1 Prupe.4G093300_v2.0.a1 Prupe.4G093400_v2.0.a1 Prupe.4G093500_v2.0.a1 Prupe.4G094100_v2.0.a1 Prupe.4G094200_v2.0.a1 Prupe.4G094300_v2.0.a1 Prupe.6G358300_v2.0.a1
pyrus_communis pycom08g08990 pycom10g20660 pycom10g20670 pycom10g20680 pycom10g20710 pycom10g20740 pycom13g00400 pycom15g08290
rosa_chinensis RchiOBHm_Chr1g0326861 RchiOBHm_Chr1g0326871 RchiOBHm_Chr1g0331391 RchiOBHm_Chr1g0336911 RchiOBHm_Chr1g0364001 RchiOBHm_Chr2g0174541 RchiOBHm_Chr2g0175041 RchiOBHm_Chr2g0175101 RchiOBHm_Chr2g0175121 RchiOBHm_Chr3g0486331 RchiOBHm_Chr5g0015581 RchiOBHm_Chr5g0016551 RchiOBHm_Chr5g0016561 RchiOBHm_Chr5g0016581 RchiOBHm_Chr5g0016601 RchiOBHm_Chr5g0016611 RchiOBHm_Chr5g0016641 RchiOBHm_Chr5g0016661 RchiOBHm_Chr5g0016691 RchiOBHm_Chr5g0016701 RchiOBHm_Chr5g0016731 RchiOBHm_Chr5g0016741 RchiOBHm_Chr5g0016811 RchiOBHm_Chr5g0016841 RchiOBHm_Chr5g0016871 RchiOBHm_Chr5g0016881 RchiOBHm_Chr5g0016931 RchiOBHm_Chr5g0017021 RchiOBHm_Chr5g0017041 RchiOBHm_Chr5g0017071 RchiOBHm_Chr5g0017091 RchiOBHm_Chr5g0017121 RchiOBHm_Chr5g0017141 RchiOBHm_Chr5g0017291 RchiOBHm_Chr5g0017321 RchiOBHm_Chr5g0017401 RchiOBHm_Chr5g0019001 RchiOBHm_Chr5g0019071 RchiOBHm_Chr5g0028941 RchiOBHm_Chr5g0052141 RchiOBHm_Chr5g0054721 RchiOBHm_Chr5g0054801 RchiOBHm_Chr5g0054931 RchiOBHm_Chr5g0054941 RchiOBHm_Chr6g0259291 RchiOBHm_Chr6g0292991 RchiOBHm_Chr6g0306911
rosa_laevigata RLG00000009465 RLG00000009468 RLG00000010755 RLG00000011976 RLG00000014541 RLG00000014542 RLG00000015169 RLG00000022308 RLG00000027529 RLG00000029324 RLG00000030244 RLG00000030245 RLG00000032210 RLG00000032211 RLG00000032257 RLG00000032259 RLG00000032260 RLG00000032261 RLG00000032262 RLG00000032268 RLG00000032269 RLG00000032272 RLG00000032282 RLG00000032283 RLG00000032308 RLG00000032413 RLG00000033129 RLG00000034945
rosa_multiflora Rmu_co8241929.1_g000001 Rmu_co8269227.1_g000001 Rmu_co8461259.1_g000001 Rmu_co8495385.1_g000001 Rmu_sc0000028.1_g000003 Rmu_sc0000083.1_g000009 Rmu_sc0000083.1_g000010 Rmu_sc0000083.1_g000031 Rmu_sc0000083.1_g000043 Rmu_sc0000083.1_g000046 Rmu_sc0000083.1_g000047 Rmu_sc0000563.1_g000024 Rmu_sc0000842.1_g000012 Rmu_sc0001235.1_g000001 Rmu_sc0001235.1_g000005 Rmu_sc0001235.1_g000009 Rmu_sc0001235.1_g000015 Rmu_sc0001235.1_g000016 Rmu_sc0001235.1_g000017 Rmu_sc0001473.1_g000036 Rmu_sc0001607.1_g000016 Rmu_sc0001788.1_g000006 Rmu_sc0002018.1_g000008 Rmu_sc0002028.1_g000001 Rmu_sc0002680.1_g000011 Rmu_sc0002798.1_g000023 Rmu_sc0002886.1_g000023 Rmu_sc0002989.1_g000012 Rmu_sc0003064.1_g000009 Rmu_sc0003382.1_g000006 Rmu_sc0003630.1_g000002 Rmu_sc0003630.1_g000047 Rmu_sc0003630.1_g000066 Rmu_sc0003630.1_g000067 Rmu_sc0003649.1_g000010 Rmu_sc0003688.1_g000005 Rmu_sc0003920.1_g000007 Rmu_sc0004765.1_g000007 Rmu_sc0004765.1_g000008 Rmu_sc0004920.1_g000011 Rmu_sc0005223.1_g000008 Rmu_sc0005223.1_g000010 Rmu_sc0005223.1_g000015 Rmu_sc0005223.1_g000016 Rmu_sc0005319.1_g000014 Rmu_sc0005319.1_g000015 Rmu_sc0005319.1_g000018 Rmu_sc0005319.1_g000019 Rmu_sc0005319.1_g000025 Rmu_sc0005418.1_g000007 Rmu_sc0005418.1_g000012 Rmu_sc0006106.1_g000011 Rmu_sc0006914.1_g000012 Rmu_sc0007023.1_g000001 Rmu_sc0007662.1_g000001 Rmu_sc0007920.1_g000002 Rmu_sc0007920.1_g000013 Rmu_sc0008053.1_g000016 Rmu_sc0008053.1_g000017 Rmu_sc0008053.1_g000018 Rmu_sc0008053.1_g000023 Rmu_sc0008053.1_g000050 Rmu_sc0009042.1_g000007 Rmu_sc0009042.1_g000008 Rmu_sc0010642.1_g000008 Rmu_sc0011109.1_g000002 Rmu_sc0011314.1_g000006 Rmu_sc0011974.1_g000006 Rmu_sc0012179.1_g000009 Rmu_sc0015169.1_g000001 Rmu_sc0016368.1_g000002 Rmu_sc0022659.1_g000001 Rmu_sc0027371.1_g000001 Rmu_sc0028213.1_g000001 Rmu_sc0034526.1_g000001 Rmu_sc0039366.1_g000002 Rmu_ssc0000050.1_g000084 Rmu_ssc0000050.1_g000088 Rmu_ssc0000167.1_g000017
rosa_roxburghii Rroxscaffold_1G00011340 Rroxscaffold_1G00025710 Rroxscaffold_1G00025870 Rroxscaffold_1G00026070 Rroxscaffold_1G00026170 Rroxscaffold_1G00026200 Rroxscaffold_1G00026270 Rroxscaffold_1G00050950 Rroxscaffold_1G00058900 Rroxscaffold_1G00060040 Rroxscaffold_1G00060060 Rroxscaffold_1G00060070 Rroxscaffold_1G00060080 Rroxscaffold_1G00060110 Rroxscaffold_1G00060120 Rroxscaffold_1G00060140 Rroxscaffold_1G00060170 Rroxscaffold_1G00060210 Rroxscaffold_1G00060220 Rroxscaffold_1G00060240 Rroxscaffold_1G00060250 Rroxscaffold_1G00060260 Rroxscaffold_1G00060300 Rroxscaffold_1G00060320 Rroxscaffold_1G00060330 Rroxscaffold_1G00060900 Rroxscaffold_1G00060920 Rroxscaffold_1G00073980 Rroxscaffold_2G00077560 Rroxscaffold_2G00077580 Rroxscaffold_2G00146990 Rroxscaffold_3G00236200 Rroxscaffold_3G00236210 Rroxscaffold_3G00236220 Rroxscaffold_4G00292760 Rroxscaffold_4G00315200 Rroxscaffold_4G00315240 Rroxscaffold_4G00326240 Rroxscaffold_4G00328980 Rroxscaffold_5G00341890 Rroxscaffold_7G00161350 Rroxscaffold_7G00174490 Rroxscaffold_7G00206550
rosa_rugosa Rorug01G0093000 Rorug01G0093100 Rorug01G0128800 Rorug01G0312000 Rorug01G0312000 Rorug02G0581100 Rorug03G0224600 Rorug05G0028500 Rorug05G0032700 Rorug05G0032800 Rorug05G0032900 Rorug05G0032900 Rorug05G0033000 Rorug05G0033100 Rorug05G0033300 Rorug05G0033400 Rorug05G0033900 Rorug05G0034900 Rorug05G0035000 Rorug05G0046900 Rorug05G0047500 Rorug05G0047500 Rorug05G0111600 Rorug05G0287900 Rorug05G0288700 Rorug05G0586900 Rorug06G0231700 Rorug06G0354300 Rorug06G0354400
rosa_samantha Rh1DG122200 Rh2DG686800 Rh5BG136600 Rh6AG344500 Rh6BG351900 Rh6CG359000 Rh6CG359100 Rh6CG387600 Rh6CG480500 Rh6DG344600 Rh6DG467400
rosa_wichuraiana Rw0G010250 Rw0G014820 Rw0G021400 Rw0G023100 Rw1G009450 Rw1G012100 Rw1G012180 Rw1G028360 Rw2G054260 Rw3G024370 Rw3G024420 Rw3G024470 Rw5G010850 Rw5G011010 Rw5G011020 Rw5G011050 Rw5G011080 Rw5G011120 Rw5G012300 Rw5G018470 Rw5G033830 Rw6G008930 Rw6G030070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1662
AccB1I GGYRCC 2 cut(s) 885, 1379
AccB7I CCANNNNNTGG 1 cut(s) 847
AccI GTMKAC 1 cut(s) 1662
AciI CCGC 6 cut(s) 5, 155, 272, 555, 950, 1125
AclWI GGATC 3 cut(s) 203, 905, 918
AcoI YGGCCR 2 cut(s) 1356, 1830
AcsI RAATTY 5 cut(s) 292, 398, 511, 943, 1776
AcyI GRCGYC 1 cut(s) 1659
AfaI GTAC 5 cut(s) 60, 571, 1166, 1976, 2059
AfeI AGCGCT 1 cut(s) 96
AfiI CCNNNNNNNGG 5 cut(s) 271, 468, 528, 822, 847
AjnI CCWGG 1 cut(s) 1611
AjuI GAANNNNNNNTTGG 4 cut(s) 383, 415, 531, 563
Alw21I GWGCWC 1 cut(s) 974
Alw26I GTCTC 3 cut(s) 644, 1223, 1808
AlwI GGATC 3 cut(s) 203, 905, 918
Ama87I CYCGRG 1 cut(s) 303
Aor51HI AGCGCT 1 cut(s) 96
AoxI GGCC 4 cut(s) 6, 1356, 1830, 1874
ApeKI GCWGC 6 cut(s) 80, 83, 97, 269, 361, 627
ApoI RAATTY 5 cut(s) 292, 398, 511, 943, 1776
AspLEI GCGC 1 cut(s) 97
AspS9I GGNCC 1 cut(s) 1615
AsuC2I CCSGG 3 cut(s) 446, 523, 670
AsuHPI GGTGA 1 cut(s) 2114
AvaI CYCGRG 1 cut(s) 303
AvaII GGWCC 1 cut(s) 1615
BaeGI GKGCMC 2 cut(s) 452, 1384
BalI TGGCCA 2 cut(s) 1358, 1832
BamHI GGATCC 1 cut(s) 910
BanI GGYRCC 2 cut(s) 885, 1379
BauI CACGAG 1 cut(s) 1283
BbsI GAAGAC 1 cut(s) 1144
Bbv12I GWGCWC 1 cut(s) 974
BbvCI CCTCAGC 1 cut(s) 322
BbvI GCAGC 6 cut(s) 84, 92, 95, 256, 348, 639
BccI CCATC 4 cut(s) 386, 875, 1570, 2099
BceAI ACGGC 4 cut(s) 374, 392, 722, 1443
BciT130I CCWGG 1 cut(s) 1613
BciVI GTATCC 1 cut(s) 974
BclI TGATCA 2 cut(s) 505, 1396
BcnI CCSGG 3 cut(s) 446, 523, 670
BcoDI GTCTC 3 cut(s) 644, 1223, 1808
BfaI CTAG 8 cut(s) 494, 552, 1209, 1439, 1595, 1682, 1904, 2040
BfmI CTRYAG 3 cut(s) 313, 589, 1663
BfoI RGCGCY 1 cut(s) 98
BfuI GTATCC 1 cut(s) 974
BisI GCNGC 7 cut(s) 6, 81, 84, 98, 270, 362, 628
BlsI GCNGC 7 cut(s) 7, 82, 85, 99, 271, 363, 629
BmcAI AGTACT 2 cut(s) 1166, 1976
Bme1390I CCNGG 4 cut(s) 446, 523, 670, 1613
Bme18I GGWCC 1 cut(s) 1615
BmeT110I CYCGRG 1 cut(s) 303
BmgT120I GGNCC 1 cut(s) 1615
BmiI GGNNCC 5 cut(s) 866, 887, 912, 1381, 1617
BmrFI CCNGG 4 cut(s) 446, 523, 670, 1613
BmrI ACTGGG 2 cut(s) 209, 477
BmsI GCATC 6 cut(s) 1023, 1504, 1715, 1746, 1838, 1990
BmuI ACTGGG 2 cut(s) 209, 477
BpiI GAAGAC 1 cut(s) 1144
Bpu10I CCTNAGC 1 cut(s) 322
BpuEI CTTGAG 3 cut(s) 75, 1280, 1873
BpuMI CCSGG 3 cut(s) 446, 523, 670
BsaBI GATNNNNATC 1 cut(s) 915
BsaHI GRCGYC 1 cut(s) 1659
BsaJI CCNNGG 7 cut(s) 169, 445, 522, 928, 1297, 1752, 1934
BsaXI ACNNNNNCTCC 6 cut(s) 1209, 1239, 1436, 1466, 1932, 1962
Bsc4I CCNNNNNNNGG 5 cut(s) 271, 468, 528, 822, 847
Bse1I ACTGG 6 cut(s) 215, 472, 585, 841, 1833, 2059
Bse3DI GCAATG 2 cut(s) 402, 1902
Bse8I GATNNNNATC 1 cut(s) 915
BseBI CCWGG 1 cut(s) 1613
BseDI CCNNGG 7 cut(s) 169, 445, 522, 928, 1297, 1752, 1934
BseGI GGATG 4 cut(s) 30, 745, 1762, 2020
BseJI GATNNNNATC 1 cut(s) 915
BseLI CCNNNNNNNGG 5 cut(s) 271, 468, 528, 822, 847
BseMI GCAATG 2 cut(s) 402, 1902
BseMII CTCAG 2 cut(s) 320, 336
BseNI ACTGG 6 cut(s) 215, 472, 585, 841, 1833, 2059
BseRI GAGGAG 2 cut(s) 1444, 2037
BseSI GKGCMC 2 cut(s) 452, 1384
BseXI GCAGC 6 cut(s) 84, 92, 95, 256, 348, 639
BshFI GGCC 4 cut(s) 8, 1358, 1832, 1876
BshNI GGYRCC 2 cut(s) 885, 1379
BsiHKAI GWGCWC 1 cut(s) 974
BsiHKCI CYCGRG 1 cut(s) 303
BsiSI CCGG 3 cut(s) 445, 522, 669
BslFI GGGAC 2 cut(s) 626, 1612
BslI CCNNNNNNNGG 5 cut(s) 271, 468, 528, 822, 847
BsmAI GTCTC 3 cut(s) 644, 1223, 1808
BsmFI GGGAC 2 cut(s) 626, 1612
BsmI GAATGC 1 cut(s) 635
BsnI GGCC 4 cut(s) 8, 1358, 1832, 1876
BsoBI CYCGRG 1 cut(s) 303
Bsp1286I GDGCHC 3 cut(s) 452, 974, 1384
Bsp143I GATC 5 cut(s) 208, 505, 910, 987, 1396
Bsp19I CCATGG 2 cut(s) 1752, 1934
BspACI CCGC 6 cut(s) 5, 155, 272, 555, 950, 1125
BspANI GGCC 4 cut(s) 8, 1358, 1832, 1876
BspCNI CTCAG 2 cut(s) 321, 335
BspLI GGNNCC 5 cut(s) 866, 887, 912, 1381, 1617
BspPI GGATC 3 cut(s) 203, 905, 918
BspT107I GGYRCC 2 cut(s) 885, 1379
BsrDI GCAATG 2 cut(s) 402, 1902
BsrI ACTGG 6 cut(s) 215, 472, 585, 841, 1833, 2059
BssECI CCNNGG 7 cut(s) 169, 445, 522, 928, 1297, 1752, 1934
BssMI GATC 5 cut(s) 208, 505, 910, 987, 1396
BssNI GRCGYC 1 cut(s) 1659
BssSI CACGAG 1 cut(s) 1283
BssT1I CCWWGG 3 cut(s) 1297, 1752, 1934
Bst2BI CACGAG 1 cut(s) 1283
Bst2UI CCWGG 1 cut(s) 1613
Bst4CI ACNGT 9 cut(s) 133, 244, 459, 569, 574, 657, 1225, 1321, 1819
Bst6I CTCTTC 1 cut(s) 642
BstACI GRCGYC 1 cut(s) 1659
BstC8I GCNNGC 2 cut(s) 791, 2075
BstDEI CTNAG 4 cut(s) 322, 329, 1507, 1716
BstDSI CCRYGG 3 cut(s) 928, 1752, 1934
BstEII GGTNACC 1 cut(s) 1607
BstF5I GGATG 4 cut(s) 30, 745, 1762, 2020
BstH2I RGCGCY 1 cut(s) 98
BstHHI GCGC 1 cut(s) 97
BstKTI GATC 5 cut(s) 211, 508, 913, 990, 1399
BstMAI GTCTC 3 cut(s) 644, 1223, 1808
BstMBI GATC 5 cut(s) 208, 505, 910, 987, 1396
BstMWI GCNNNNNNNGC 6 cut(s) 92, 361, 498, 1523, 1734, 1838
BstNI CCWGG 1 cut(s) 1613
BstNSI RCATGY 2 cut(s) 478, 2077
BstPI GGTNACC 1 cut(s) 1607
BstSCI CCNGG 4 cut(s) 444, 521, 668, 1611
BstSFI CTRYAG 3 cut(s) 313, 589, 1663
BstSLI GKGCMC 2 cut(s) 452, 1384
BstV1I GCAGC 6 cut(s) 84, 92, 95, 256, 348, 639
BstV2I GAAGAC 1 cut(s) 1144
BstX2I RGATCY 1 cut(s) 910
BstXI CCANNNNNNTGG 1 cut(s) 881
BstYI RGATCY 1 cut(s) 910
BsuI GTATCC 1 cut(s) 974
BsuRI GGCC 4 cut(s) 8, 1358, 1832, 1876
BtgI CCRYGG 3 cut(s) 928, 1752, 1934
BtsCI GGATG 4 cut(s) 30, 745, 1762, 2020
BtsIMutI CAGTG 4 cut(s) 240, 578, 848, 1221
Cac8I GCNNGC 2 cut(s) 791, 2075
CfoI GCGC 1 cut(s) 97
Cfr13I GGNCC 1 cut(s) 1615
Csp6I GTAC 5 cut(s) 59, 570, 1165, 1975, 2058
CviAII CATG 8 cut(s) 151, 475, 753, 1214, 1753, 1882, 1935, 2074
CviQI GTAC 5 cut(s) 59, 570, 1165, 1975, 2058
DdeI CTNAG 4 cut(s) 322, 329, 1507, 1716
DpnI GATC 5 cut(s) 210, 507, 912, 989, 1398
DpnII GATC 5 cut(s) 208, 505, 910, 987, 1396
EaeI YGGCCR 2 cut(s) 1356, 1830
Eam1104I CTCTTC 1 cut(s) 642
EarI CTCTTC 1 cut(s) 642
EciI GGCGGA 1 cut(s) 170
Eco130I CCWWGG 3 cut(s) 1297, 1752, 1934
Eco147I AGGCCT 1 cut(s) 1876
Eco47I GGWCC 1 cut(s) 1615
Eco47III AGCGCT 1 cut(s) 96
Eco88I CYCGRG 1 cut(s) 303
Eco91I GGTNACC 1 cut(s) 1607
EcoO65I GGTNACC 1 cut(s) 1607
EcoRI GAATTC 1 cut(s) 511
EcoRII CCWGG 1 cut(s) 1611
EcoT14I CCWWGG 3 cut(s) 1297, 1752, 1934
ErhI CCWWGG 3 cut(s) 1297, 1752, 1934
FaeI CATG 8 cut(s) 154, 478, 756, 1217, 1756, 1885, 1938, 2077
FalI AAGNNNNNCTT 2 cut(s) 946, 978
FaqI GGGAC 2 cut(s) 626, 1612
FatI CATG 8 cut(s) 150, 474, 752, 1213, 1752, 1881, 1934, 2073
FauI CCCGC 3 cut(s) 265, 943, 1118
FbaI TGATCA 2 cut(s) 505, 1396
FblI GTMKAC 1 cut(s) 1662
Fnu4HI GCNGC 7 cut(s) 6, 81, 84, 98, 270, 362, 628
FokI GGATG 4 cut(s) 17, 752, 1769, 2027
Fsp4HI GCNGC 7 cut(s) 6, 81, 84, 98, 270, 362, 628
FspBI CTAG 8 cut(s) 494, 552, 1209, 1439, 1595, 1682, 1904, 2040
GlaI GCGC 1 cut(s) 96
GluI GCNGC 7 cut(s) 6, 81, 84, 98, 270, 362, 628
HaeII RGCGCY 1 cut(s) 98
HaeIII GGCC 4 cut(s) 8, 1358, 1832, 1876
HapII CCGG 3 cut(s) 445, 522, 669
HhaI GCGC 1 cut(s) 97
Hin1I GRCGYC 1 cut(s) 1659
Hin1II CATG 8 cut(s) 154, 478, 756, 1217, 1756, 1885, 1938, 2077
Hin6I GCGC 1 cut(s) 95
HinP1I GCGC 1 cut(s) 95
HincII GTYRAC 1 cut(s) 1408
HindII GTYRAC 1 cut(s) 1408
HinfI GANTC 8 cut(s) 441, 811, 1024, 1048, 1293, 1558, 1771, 1994
HpaI GTTAAC 1 cut(s) 1408
HpaII CCGG 3 cut(s) 445, 522, 669
HphI GGTGA 1 cut(s) 2114
Hpy166II GTNNAC 3 cut(s) 1324, 1408, 1663
Hpy188I TCNGA 5 cut(s) 330, 385, 510, 1537, 1999
Hpy188III TCNNGA 5 cut(s) 305, 1481, 1551, 1781, 1961
Hpy8I GTNNAC 3 cut(s) 1324, 1408, 1663
HpyAV CCTTC 8 cut(s) 152, 190, 333, 375, 845, 854, 1258, 1298
HpyCH4III ACNGT 9 cut(s) 133, 244, 459, 569, 574, 657, 1225, 1321, 1819
HpyCH4IV ACGT 1 cut(s) 1659
HpyF10VI GCNNNNNNNGC 6 cut(s) 92, 361, 498, 1523, 1734, 1838
HpyF3I CTNAG 4 cut(s) 322, 329, 1507, 1716
HpySE526I ACGT 1 cut(s) 1659
Hsp92I GRCGYC 1 cut(s) 1659
Hsp92II CATG 8 cut(s) 154, 478, 756, 1217, 1756, 1885, 1938, 2077
HspAI GCGC 1 cut(s) 95
Ksp22I TGATCA 2 cut(s) 505, 1396
KspAI GTTAAC 1 cut(s) 1408
Kzo9I GATC 5 cut(s) 208, 505, 910, 987, 1396
LmnI GCTCC 5 cut(s) 524, 1544, 1687, 1900, 1940
Lsp1109I GCAGC 6 cut(s) 84, 92, 95, 256, 348, 639
LweI GCATC 6 cut(s) 1023, 1504, 1715, 1746, 1838, 1990
MaeI CTAG 8 cut(s) 494, 552, 1209, 1439, 1595, 1682, 1904, 2040
MaeII ACGT 1 cut(s) 1659
MalI GATC 5 cut(s) 210, 507, 912, 989, 1398
MboI GATC 5 cut(s) 208, 505, 910, 987, 1396
MboII GAAGA 5 cut(s) 659, 1149, 1265, 1740, 1772
MfeI CAATTG 2 cut(s) 190, 732
MflI RGATCY 1 cut(s) 910
MhlI GDGCHC 3 cut(s) 452, 974, 1384
MlsI TGGCCA 2 cut(s) 1358, 1832
MluNI TGGCCA 2 cut(s) 1358, 1832
MlyI GAGTC 1 cut(s) 1042
Mox20I TGGCCA 2 cut(s) 1358, 1832
MscI TGGCCA 2 cut(s) 1358, 1832
MseI TTAA 6 cut(s) 618, 723, 1070, 1407, 1730, 2048
MslI CAYNNNNRTG 2 cut(s) 879, 1482
Msp20I TGGCCA 2 cut(s) 1358, 1832
MspI CCGG 3 cut(s) 445, 522, 669
MspR9I CCNGG 4 cut(s) 446, 523, 670, 1613
MunI CAATTG 2 cut(s) 190, 732
Mva1269I GAATGC 1 cut(s) 635
MvaI CCWGG 1 cut(s) 1613
MwoI GCNNNNNNNGC 6 cut(s) 92, 361, 498, 1523, 1734, 1838
NciI CCSGG 3 cut(s) 446, 523, 670
NcoI CCATGG 2 cut(s) 1752, 1934
NdeII GATC 5 cut(s) 208, 505, 910, 987, 1396
NlaIII CATG 8 cut(s) 154, 478, 756, 1217, 1756, 1885, 1938, 2077
NlaIV GGNNCC 5 cut(s) 866, 887, 912, 1381, 1617
NmuCI GTSAC 4 cut(s) 416, 574, 1655, 1986
NspI RCATGY 2 cut(s) 478, 2077
PaeI GCATGC 1 cut(s) 2077
PaeR7I CTCGAG 1 cut(s) 303
PceI AGGCCT 1 cut(s) 1876
PctI GAATGC 1 cut(s) 635
PfeI GAWTC 7 cut(s) 441, 811, 1024, 1293, 1558, 1771, 1994
PflMI CCANNNNNTGG 1 cut(s) 847
PfoI TCCNGGA 1 cut(s) 668
PkrI GCNGC 7 cut(s) 7, 82, 85, 99, 271, 363, 629
PleI GAGTC 1 cut(s) 1042
PpsI GAGTC 1 cut(s) 1042
Psp6I CCWGG 1 cut(s) 1611
PspEI GGTNACC 1 cut(s) 1607
PspGI CCWGG 1 cut(s) 1611
PspN4I GGNNCC 5 cut(s) 866, 887, 912, 1381, 1617
PspPI GGNCC 1 cut(s) 1615
PsuI RGATCY 1 cut(s) 910
RsaI GTAC 5 cut(s) 60, 571, 1166, 1976, 2059
RsaNI GTAC 5 cut(s) 59, 570, 1165, 1975, 2058
RseI CAYNNNNRTG 2 cut(s) 879, 1482
SaqAI TTAA 6 cut(s) 618, 723, 1070, 1407, 1730, 2048
SatI GCNGC 7 cut(s) 6, 81, 84, 98, 270, 362, 628
Sau3AI GATC 5 cut(s) 208, 505, 910, 987, 1396
Sau96I GGNCC 1 cut(s) 1615
ScaI AGTACT 2 cut(s) 1166, 1976
SchI GAGTC 1 cut(s) 1042
ScrFI CCNGG 4 cut(s) 446, 523, 670, 1613
SduI GDGCHC 3 cut(s) 452, 974, 1384
SfaNI GCATC 6 cut(s) 1023, 1504, 1715, 1746, 1838, 1990
SfcI CTRYAG 3 cut(s) 313, 589, 1663
Sfr274I CTCGAG 1 cut(s) 303
SinI GGWCC 1 cut(s) 1615
SlaI CTCGAG 1 cut(s) 303
SmiMI CAYNNNNRTG 2 cut(s) 879, 1482
SmlI CTYRAG 4 cut(s) 90, 303, 1259, 1852
SmoI CTYRAG 4 cut(s) 90, 303, 1259, 1852
SphI GCATGC 1 cut(s) 2077
SseBI AGGCCT 1 cut(s) 1876
SsiI CCGC 6 cut(s) 5, 155, 272, 555, 950, 1125
SspMI CTAG 8 cut(s) 494, 552, 1209, 1439, 1595, 1682, 1904, 2040
StuI AGGCCT 1 cut(s) 1876
StyD4I CCNGG 4 cut(s) 444, 521, 668, 1611
StyI CCWWGG 3 cut(s) 1297, 1752, 1934
TaaI ACNGT 9 cut(s) 133, 244, 459, 569, 574, 657, 1225, 1321, 1819
TaiI ACGT 1 cut(s) 1662
TaqI TCGA 4 cut(s) 304, 990, 1480, 1634
TatI WGTACW 2 cut(s) 1164, 1974
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 7 cut(s) 441, 811, 1024, 1293, 1558, 1771, 1994
Tru1I TTAA 6 cut(s) 618, 723, 1070, 1407, 1730, 2048
Tru9I TTAA 6 cut(s) 618, 723, 1070, 1407, 1730, 2048
TscAI CASTG 4 cut(s) 247, 585, 848, 1228
TseFI GTSAC 4 cut(s) 416, 574, 1655, 1986
TseI GCWGC 6 cut(s) 80, 83, 97, 269, 361, 627
Tsp45I GTSAC 4 cut(s) 416, 574, 1655, 1986
TspDTI ATGAA 6 cut(s) 391, 628, 873, 1056, 1773, 1898
TspGWI ACGGA 1 cut(s) 945
TspRI CASTG 4 cut(s) 247, 585, 848, 1228
Van91I CCANNNNNTGG 1 cut(s) 847
VpaK11BI GGWCC 1 cut(s) 1615
XapI RAATTY 5 cut(s) 292, 398, 511, 943, 1776
XceI RCATGY 2 cut(s) 478, 2077
XhoI CTCGAG 1 cut(s) 303
XmiI GTMKAC 1 cut(s) 1662
XspI CTAG 8 cut(s) 494, 552, 1209, 1439, 1595, 1682, 1904, 2040
ZraI GACGTC 1 cut(s) 1660
ZrmI AGTACT 2 cut(s) 1166, 1976
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.