MD10G1251200.v1.1

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000066
Physical Location & Seq
Forward (+)
34454093 .. 34459942
5850 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1251200.v1.1.491

Sequence Viewer

Length: 2301 bp
ATGGCGTTACATAGGAGGATGCATATTTTGCATCTCTCTTTAGTAATGGTGGTAGTTGTTACAATTTTACTAGCAACTCCAACTCCAACAACAGCTCAATCTCTCCTTCCGCCTCAGCTGGCACGGCCTGGCTGCCCTAACCATTGTGGTAATCTCACAATTCCATACCCATTTGGCATAGGCTCTGCTTGCTACCTCGACCACCGGTTTGAAATCACTTGTGACATTTCCACTCAACCGCCAACAGCACGTTTGTTGACGGGCAATGTCAAAGAAATTACCAACATATCTCTTTATGACGGTGAGCTCCAAATACTACAGTTCGCAGCCCGTGATTGTTATGATGCTCAAGGCAACAGGACCGAATATAACCGCTCCGAGCTCTGGGTGTCTCCTCCTTACACCATCTCCAAAACCAAAAACATGTTCGTTGCGGTTGGATGCGACACATATGCGCTTTTTAAAGGTTACCAGGGCGAAGAAATGCACAGAACTAGGTGTATGTCCATATGTGACAATCTTACCAACGCTACCGATCAGTTGGATACTTGCTCTGGCGTTGGATGTTGCCAAACTAAAATCCCCGGTGGGCTGAAAAATGGGACTGTGTACTTGGGAAGCTTTGACAATTATTATTTTGTGGGGGACTCCAACCGATGCAGCTACGCGTTCCTTGTGCAAGAAGACGAGTTCAACTTTACAAGTACAAGTCTTCGAGAACTAAACAATACAACCCGGCTTCCGGCAATTCTTGATTGGGAAATTGGGAATGAGTTATGTGACATTGCAGCTAAAAACAAGAGCACTTTTGCATGCAAGGCAAATAGCACCTGCGATAATCGGTCTTCAGGCTACATATGTCGGTGCCGGCCAGGTTACGAAGGGAACCCATACCTCCCAGATGGTTGCCGAGATATTGATGAGTGCGAGGCTCCAGACTCCTGCAGTACTGGAACCTGCATAAATACAGCGGGAAGTTACACTTGTAAATGTCCGAAAGGGTACAAAAATGACGATCAGGATAAAAAAAAGTTGCATCAAGGACGACGGTCGAGCAAAGCTCAGTCTCCTGCTTATTATTTCACTAGTGGAGGCTTCTTGCTTTTACTAAGTGGATCTTCTTGGATGTATTGGGGAATGCAGAGAAGAAAACTCAAAGAAAAGTACTTTAAAGAAAATGGTGGCTTATTGTTACAACAAAAAATCGCTAGTGAAGGAGGCTCTGTGGAGACAACAAAAATTTTCACAGCAGAAGAACTTGAGAAGGCAACAAACAATTACCATGAAAGTAGAATCCTTGGTGAAGGAGGCTATGGAATAGTTTACAAAGGAATATTAGCAGCAGATAACAATAAAGTGGTTGCCATAAAAAAGTCTAAAATTTGTGTCCTAACGCAGAAGGAGCATTTTGTCAACGAGTTGCTTGTTCTTTCTCAAATCAACCATAGAAATGTGGTGAGGTTATTGGGTTGTTGTTTGGAGTTAGAAGTTCCTCTACTAGTTTACGAGTTTGTCGCTCACGGCACTCTCTTTGAGCACATACATGGCAAAAAGAGGAAAGGATCATCATTTTCATTGGAATTACGACTCAAGATAGCAGCTGAAACTGCTGGAGCACTAGCATACTTACACTCCTCAGCTCTGATGCAAATCATACATCGAGATGTGAAACTGACAAATATACTGTTGGATGAAAATTACACGGCAAAAGTGTCAGACTTTGGAGCTTCACGATTGATTCCTCTTGATCAAGCCCAGCTTACAACTTTAGTGCAGGGTACATTCGGATACTTAGACCCTGAATACTTCCTCACGAATCAACTAACAGATAAGAGTGACGTCTATAGCTTTGGAGTTGTCCTTATGGAGCTACTAACAAGCAAAGTGGCACTTGCTTTTGACAGGCCTGAGGAAGAGAGAAACCTAGCAAACTTCTTTGTTTGTTTAATGGAAGAGGATCGCCTGAATGAAATTCTCGATGATGACATACTCAACGAGAGAAACATCGAGACAATAAAAACAGTGGCCAATCTAGCAAAAAGATGTGTAAGGTTAAAAGGGAAGGACAGGCCAACAATGAAAGAAGCTGCCATGGAGCTGGAAGGAATGAGAATCACAGAAAAGCATCCATGGGGAAAAGCTGAAATTTGTTCAGAAGAGACTGAGTGCTTACTTGGGCGAGTTAACTCAGATGCTTATCCTGTGGATGTTAGAGTTGATTGTGGTCCTAGTACTGGTACAACTATTGGGTATGACAGCATGCAGATCCAGCTGTTAATTCCATATGATGATGGAAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK

Protein Analysis

767

Amino Acids

85.54

Weight (kDa)

5.97

Isoelectric Point (pI)

39.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 45 - 105 2.4e-10 Wall-associated receptor kinase galacturonan-binding
EGF_CA PF07645 305 - 342 5.8e-10 Calcium-binding EGF domain
Pkinase PF00069 427 - 696 4.3e-46 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 430 - 698 5.1e-45 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21210 AT1G21230 AT1G21240 AT1G21240 AT1G21245 AT1G21250 AT1G21270 AT1G22720 AT3G25490
fragaria_vesca FvH4_2g35051 FvH4_2g35051 FvH4_2g35060 FvH4_3g10281 FvH4_3g10285 FvH4_3g10286 FvH4_3g10311 FvH4_3g10311 FvH4_3g10320 FvH4_3g10332 FvH4_3g10340 FvH4_3g10350 FvH4_3g10360 FvH4_3g10390 FvH4_3g10412 FvH4_3g10413 FvH4_3g10461 FvH4_3g10462 FvH4_3g10470 FvH4_3g10510 FvH4_3g10520 FvH4_3g10530 FvH4_3g10550 FvH4_3g10560 FvH4_3g10611 FvH4_3g11730 FvH4_6g53140 FvH4_6g53142 FvH4_7g31880 FvH4_7g31910 FvH4_7g31920
malus_domestica MD05G1269900.v1.1 MD05G1270000.v1.1 MD05G1270200.v1.1 MD05G1270400.v1.1 MD05G1270500.v1.1 MD05G1270600.v1.1 MD05G1270800.v1.1 MD05G1270900.v1.1 MD07G1183800.v1.1 MD08G1107600.v1.1 MD10G1248500.v1.1 MD10G1249200.v1.1 MD10G1249500.v1.1 MD10G1250000.v1.1 MD10G1250500.v1.1 MD10G1250900.v1.1 MD10G1251200.v1.1 MD10G1251400.v1.1 MD13G1004600.v1.1 MD15G1088400.v1.1
prunus_persica Prupe.1G442400_v2.0.a1 Prupe.1G442500_v2.0.a1 Prupe.2G223400_v2.0.a1 Prupe.4G091900_v2.0.a1 Prupe.4G093200_v2.0.a1 Prupe.4G093300_v2.0.a1 Prupe.4G093400_v2.0.a1 Prupe.4G093500_v2.0.a1 Prupe.4G094100_v2.0.a1 Prupe.4G094200_v2.0.a1 Prupe.4G094300_v2.0.a1 Prupe.6G358300_v2.0.a1
pyrus_communis pycom08g08990 pycom10g20660 pycom10g20670 pycom10g20680 pycom10g20710 pycom10g20740 pycom13g00400 pycom15g08290
rosa_chinensis RchiOBHm_Chr1g0326861 RchiOBHm_Chr1g0326871 RchiOBHm_Chr1g0331391 RchiOBHm_Chr1g0336911 RchiOBHm_Chr1g0364001 RchiOBHm_Chr2g0174541 RchiOBHm_Chr2g0175041 RchiOBHm_Chr2g0175101 RchiOBHm_Chr2g0175121 RchiOBHm_Chr3g0486331 RchiOBHm_Chr5g0015581 RchiOBHm_Chr5g0016551 RchiOBHm_Chr5g0016561 RchiOBHm_Chr5g0016581 RchiOBHm_Chr5g0016601 RchiOBHm_Chr5g0016611 RchiOBHm_Chr5g0016641 RchiOBHm_Chr5g0016661 RchiOBHm_Chr5g0016691 RchiOBHm_Chr5g0016701 RchiOBHm_Chr5g0016731 RchiOBHm_Chr5g0016741 RchiOBHm_Chr5g0016811 RchiOBHm_Chr5g0016841 RchiOBHm_Chr5g0016871 RchiOBHm_Chr5g0016881 RchiOBHm_Chr5g0016931 RchiOBHm_Chr5g0017021 RchiOBHm_Chr5g0017041 RchiOBHm_Chr5g0017071 RchiOBHm_Chr5g0017091 RchiOBHm_Chr5g0017121 RchiOBHm_Chr5g0017141 RchiOBHm_Chr5g0017291 RchiOBHm_Chr5g0017321 RchiOBHm_Chr5g0017401 RchiOBHm_Chr5g0019001 RchiOBHm_Chr5g0019071 RchiOBHm_Chr5g0028941 RchiOBHm_Chr5g0052141 RchiOBHm_Chr5g0054721 RchiOBHm_Chr5g0054801 RchiOBHm_Chr5g0054931 RchiOBHm_Chr5g0054941 RchiOBHm_Chr6g0259291 RchiOBHm_Chr6g0292991 RchiOBHm_Chr6g0306911
rosa_laevigata RLG00000009465 RLG00000009468 RLG00000010755 RLG00000011976 RLG00000014541 RLG00000014542 RLG00000015169 RLG00000022308 RLG00000027529 RLG00000029324 RLG00000030244 RLG00000030245 RLG00000032210 RLG00000032211 RLG00000032257 RLG00000032259 RLG00000032260 RLG00000032261 RLG00000032262 RLG00000032268 RLG00000032269 RLG00000032272 RLG00000032282 RLG00000032283 RLG00000032308 RLG00000032413 RLG00000033129 RLG00000034945
rosa_multiflora Rmu_co8241929.1_g000001 Rmu_co8269227.1_g000001 Rmu_co8461259.1_g000001 Rmu_co8495385.1_g000001 Rmu_sc0000028.1_g000003 Rmu_sc0000083.1_g000009 Rmu_sc0000083.1_g000010 Rmu_sc0000083.1_g000031 Rmu_sc0000083.1_g000043 Rmu_sc0000083.1_g000046 Rmu_sc0000083.1_g000047 Rmu_sc0000563.1_g000024 Rmu_sc0000842.1_g000012 Rmu_sc0001235.1_g000001 Rmu_sc0001235.1_g000005 Rmu_sc0001235.1_g000009 Rmu_sc0001235.1_g000015 Rmu_sc0001235.1_g000016 Rmu_sc0001235.1_g000017 Rmu_sc0001473.1_g000036 Rmu_sc0001607.1_g000016 Rmu_sc0001788.1_g000006 Rmu_sc0002018.1_g000008 Rmu_sc0002028.1_g000001 Rmu_sc0002680.1_g000011 Rmu_sc0002798.1_g000023 Rmu_sc0002886.1_g000023 Rmu_sc0002989.1_g000012 Rmu_sc0003064.1_g000009 Rmu_sc0003382.1_g000006 Rmu_sc0003630.1_g000002 Rmu_sc0003630.1_g000047 Rmu_sc0003630.1_g000066 Rmu_sc0003630.1_g000067 Rmu_sc0003649.1_g000010 Rmu_sc0003688.1_g000005 Rmu_sc0003920.1_g000007 Rmu_sc0004765.1_g000007 Rmu_sc0004765.1_g000008 Rmu_sc0004920.1_g000011 Rmu_sc0005223.1_g000008 Rmu_sc0005223.1_g000010 Rmu_sc0005223.1_g000015 Rmu_sc0005223.1_g000016 Rmu_sc0005319.1_g000014 Rmu_sc0005319.1_g000015 Rmu_sc0005319.1_g000018 Rmu_sc0005319.1_g000019 Rmu_sc0005319.1_g000025 Rmu_sc0005418.1_g000007 Rmu_sc0005418.1_g000012 Rmu_sc0006106.1_g000011 Rmu_sc0006914.1_g000012 Rmu_sc0007023.1_g000001 Rmu_sc0007662.1_g000001 Rmu_sc0007920.1_g000002 Rmu_sc0007920.1_g000013 Rmu_sc0008053.1_g000016 Rmu_sc0008053.1_g000017 Rmu_sc0008053.1_g000018 Rmu_sc0008053.1_g000023 Rmu_sc0008053.1_g000050 Rmu_sc0009042.1_g000007 Rmu_sc0009042.1_g000008 Rmu_sc0010642.1_g000008 Rmu_sc0011109.1_g000002 Rmu_sc0011314.1_g000006 Rmu_sc0011974.1_g000006 Rmu_sc0012179.1_g000009 Rmu_sc0015169.1_g000001 Rmu_sc0016368.1_g000002 Rmu_sc0022659.1_g000001 Rmu_sc0027371.1_g000001 Rmu_sc0028213.1_g000001 Rmu_sc0034526.1_g000001 Rmu_sc0039366.1_g000002 Rmu_ssc0000050.1_g000084 Rmu_ssc0000050.1_g000088 Rmu_ssc0000167.1_g000017
rosa_roxburghii Rroxscaffold_1G00011340 Rroxscaffold_1G00025710 Rroxscaffold_1G00025870 Rroxscaffold_1G00026070 Rroxscaffold_1G00026170 Rroxscaffold_1G00026200 Rroxscaffold_1G00026270 Rroxscaffold_1G00050950 Rroxscaffold_1G00058900 Rroxscaffold_1G00060040 Rroxscaffold_1G00060060 Rroxscaffold_1G00060070 Rroxscaffold_1G00060080 Rroxscaffold_1G00060110 Rroxscaffold_1G00060120 Rroxscaffold_1G00060140 Rroxscaffold_1G00060170 Rroxscaffold_1G00060210 Rroxscaffold_1G00060220 Rroxscaffold_1G00060240 Rroxscaffold_1G00060250 Rroxscaffold_1G00060260 Rroxscaffold_1G00060300 Rroxscaffold_1G00060320 Rroxscaffold_1G00060330 Rroxscaffold_1G00060900 Rroxscaffold_1G00060920 Rroxscaffold_1G00073980 Rroxscaffold_2G00077560 Rroxscaffold_2G00077580 Rroxscaffold_2G00146990 Rroxscaffold_3G00236200 Rroxscaffold_3G00236210 Rroxscaffold_3G00236220 Rroxscaffold_4G00292760 Rroxscaffold_4G00315200 Rroxscaffold_4G00315240 Rroxscaffold_4G00326240 Rroxscaffold_4G00328980 Rroxscaffold_5G00341890 Rroxscaffold_7G00161350 Rroxscaffold_7G00174490 Rroxscaffold_7G00206550
rosa_rugosa Rorug01G0093000 Rorug01G0093100 Rorug01G0128800 Rorug01G0312000 Rorug01G0312000 Rorug02G0581100 Rorug03G0224600 Rorug05G0028500 Rorug05G0032700 Rorug05G0032800 Rorug05G0032900 Rorug05G0032900 Rorug05G0033000 Rorug05G0033100 Rorug05G0033300 Rorug05G0033400 Rorug05G0033900 Rorug05G0034900 Rorug05G0035000 Rorug05G0046900 Rorug05G0047500 Rorug05G0047500 Rorug05G0111600 Rorug05G0287900 Rorug05G0288700 Rorug05G0586900 Rorug06G0231700 Rorug06G0354300 Rorug06G0354400
rosa_samantha Rh1DG122200 Rh2DG686800 Rh5BG136600 Rh6AG344500 Rh6BG351900 Rh6CG359000 Rh6CG359100 Rh6CG387600 Rh6CG480500 Rh6DG344600 Rh6DG467400
rosa_wichuraiana Rw0G010250 Rw0G014820 Rw0G021400 Rw0G023100 Rw1G009450 Rw1G012100 Rw1G012180 Rw1G028360 Rw2G054260 Rw3G024370 Rw3G024420 Rw3G024470 Rw5G010850 Rw5G011010 Rw5G011020 Rw5G011050 Rw5G011080 Rw5G011120 Rw5G012300 Rw5G018470 Rw5G033830 Rw6G008930 Rw6G030070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 839
AatII GACGTC 1 cut(s) 1842
Acc36I ACCTGC 2 cut(s) 839, 965
AccB1I GGYRCC 1 cut(s) 864
AccBSI CCGCTC 1 cut(s) 375
AccII CGCG 1 cut(s) 668
AciI CCGC 5 cut(s) 110, 239, 373, 434, 971
AclWI GGATC 4 cut(s) 1123, 1570, 1965, 2260
AcoI YGGCCR 2 cut(s) 869, 2025
AcsI RAATTY 4 cut(s) 1239, 1380, 1971, 2145
AcuI CTGAAG 1 cut(s) 831
AcyI GRCGYC 1 cut(s) 1839
AfaI GTAC 8 cut(s) 611, 706, 949, 1004, 1166, 1780, 2233, 2239
AfiI CCNNNNNNNGG 3 cut(s) 384, 742, 2234
AflIII ACRYGT 2 cut(s) 423, 666
AgeI ACCGGT 1 cut(s) 204
AgsI TTSAA 2 cut(s) 212, 694
AhlI ACTAGT 2 cut(s) 1085, 1498
AjnI CCWGG 3 cut(s) 127, 471, 871
AjuI GAANNNNNNNTTGG 2 cut(s) 410, 442
Alw21I GWGCWC 5 cut(s) 309, 384, 806, 1539, 1618
Alw26I GTCTC 5 cut(s) 396, 1071, 1223, 2003, 2153
AlwI GGATC 4 cut(s) 1123, 1570, 1965, 2260
AoxI GGCC 5 cut(s) 125, 869, 1904, 2025, 2069
ApeKI GCWGC 7 cut(s) 132, 326, 660, 788, 1340, 1598, 2087
ApoI RAATTY 4 cut(s) 1239, 1380, 1971, 2145
ArsI GACNNNNNNTTYG 2 cut(s) 191, 223
AsiGI ACCGGT 1 cut(s) 204
AspLEI GCGC 1 cut(s) 457
AspS9I GGNCC 2 cut(s) 360, 2225
AsuC2I CCSGG 2 cut(s) 585, 736
AsuHPI GGTGA 3 cut(s) 314, 1313, 1468
AvaII GGWCC 2 cut(s) 360, 2225
AxyI CCTNAGG 1 cut(s) 1908
BalI TGGCCA 1 cut(s) 2027
BanI GGYRCC 1 cut(s) 864
BanII GRGCYC 2 cut(s) 309, 384
BarI GAAGNNNNNNTAC 2 cut(s) 1479, 1511
BbsI GAAGAC 3 cut(s) 690, 704, 837
Bbv12I GWGCWC 5 cut(s) 309, 384, 806, 1539, 1618
BbvCI CCTCAGC 2 cut(s) 114, 1636
BbvI GCAGC 7 cut(s) 119, 338, 672, 800, 1352, 1610, 2074
BccI CCATC 3 cut(s) 413, 896, 2285
BceAI ACGGC 3 cut(s) 140, 1537, 1719
BcgI CGANNNNNNTGC 2 cut(s) 434, 468
BciT130I CCWGG 3 cut(s) 129, 473, 873
BciVI GTATCC 2 cut(s) 538, 1781
BclI TGATCA 1 cut(s) 1747
BcnI CCSGG 2 cut(s) 585, 736
BcoDI GTCTC 5 cut(s) 396, 1071, 1223, 2003, 2153
BcuI ACTAGT 2 cut(s) 1085, 1498
BfaI CTAG 9 cut(s) 71, 495, 1086, 1209, 1499, 1619, 1925, 2033, 2229
BfmI CTRYAG 3 cut(s) 317, 943, 1843
BfuAI ACCTGC 2 cut(s) 839, 965
BfuI GTATCC 2 cut(s) 538, 1781
BisI GCNGC 7 cut(s) 133, 327, 661, 789, 1341, 1599, 2088
BlsI GCNGC 7 cut(s) 134, 328, 662, 790, 1342, 1600, 2089
BmcAI AGTACT 3 cut(s) 949, 1166, 2233
Bme1390I CCNGG 5 cut(s) 129, 473, 585, 736, 873
Bme18I GGWCC 2 cut(s) 360, 2225
BmgT120I GGNCC 2 cut(s) 360, 2225
BmiI GGNNCC 4 cut(s) 866, 887, 933, 955
BmrFI CCNGG 5 cut(s) 129, 473, 585, 736, 873
BmsI GCATC 9 cut(s) 9, 40, 334, 431, 647, 1045, 1635, 2134, 2182
BoxI GACNNNNGTC 1 cut(s) 1048
BpiI GAAGAC 3 cut(s) 690, 704, 837
BplI GAGNNNNNCTC 2 cut(s) 1045, 1077
BpmI CTGGAG 2 cut(s) 918, 1632
Bpu10I CCTNAGC 2 cut(s) 114, 1636
BpuEI CTTGAG 3 cut(s) 333, 1280, 1574
BpuMI CCSGG 2 cut(s) 585, 736
BsaBI GATNNNNATC 2 cut(s) 1649, 2196
BsaHI GRCGYC 1 cut(s) 1839
BsaJI CCNNGG 5 cut(s) 472, 583, 1297, 2091, 2129
BsaWI WCCGGW 1 cut(s) 204
BsaXI ACNNNNNCTCC 8 cut(s) 67, 97, 392, 422, 1209, 1239, 1616, 1646
Bsc4I CCNNNNNNNGG 3 cut(s) 384, 742, 2234
Bse118I RCCGGY 2 cut(s) 204, 867
Bse1I ACTGG 2 cut(s) 955, 2239
Bse21I CCTNAGG 1 cut(s) 1908
Bse3DI GCAATG 2 cut(s) 271, 783
Bse8I GATNNNNATC 2 cut(s) 1649, 2196
BseBI CCWGG 3 cut(s) 129, 473, 873
BseDI CCNNGG 5 cut(s) 472, 583, 1297, 2091, 2129
BseGI GGATG 7 cut(s) 24, 446, 569, 1131, 1696, 2125, 2212
BseJI GATNNNNATC 2 cut(s) 1649, 2196
BseLI CCNNNNNNNGG 3 cut(s) 384, 742, 2234
BseMI GCAATG 2 cut(s) 271, 783
BseMII CTCAG 6 cut(s) 128, 1076, 1650, 1899, 2154, 2202
BseNI ACTGG 2 cut(s) 955, 2239
BseRI GAGGAG 2 cut(s) 384, 1624
BseXI GCAGC 7 cut(s) 119, 338, 672, 800, 1352, 1610, 2074
BseYI CCCAGC 1 cut(s) 1755
BsgI GTGCAG 1 cut(s) 1793
Bsh1236I CGCG 1 cut(s) 668
Bsh1285I CGRYCG 1 cut(s) 1052
BshFI GGCC 5 cut(s) 127, 871, 1906, 2027, 2071
BshNI GGYRCC 1 cut(s) 864
BshTI ACCGGT 1 cut(s) 204
BsiEI CGRYCG 1 cut(s) 1052
BsiHKAI GWGCWC 5 cut(s) 309, 384, 806, 1539, 1618
BsiSI CCGG 5 cut(s) 205, 585, 736, 743, 868
BslFI GGGAC 2 cut(s) 616, 659
BslI CCNNNNNNNGG 3 cut(s) 384, 742, 2234
BsmAI GTCTC 5 cut(s) 396, 1071, 1223, 2003, 2153
BsmFI GGGAC 2 cut(s) 616, 659
BsmI GAATGC 1 cut(s) 1143
BsnI GGCC 5 cut(s) 127, 871, 1906, 2027, 2071
Bsp1286I GDGCHC 5 cut(s) 309, 384, 806, 1539, 1618
Bsp143I GATC 7 cut(s) 535, 1015, 1115, 1562, 1747, 1957, 2265
Bsp19I CCATGG 2 cut(s) 2091, 2129
BspACI CCGC 5 cut(s) 110, 239, 373, 434, 971
BspANI GGCC 5 cut(s) 127, 871, 1906, 2027, 2071
BspCNI CTCAG 6 cut(s) 127, 1075, 1649, 1900, 2155, 2201
BspFNI CGCG 1 cut(s) 668
BspLI GGNNCC 4 cut(s) 866, 887, 933, 955
BspMAI CTGCAG 1 cut(s) 947
BspMI ACCTGC 2 cut(s) 839, 965
BspPI GGATC 4 cut(s) 1123, 1570, 1965, 2260
BspT107I GGYRCC 1 cut(s) 864
BsrBI CCGCTC 1 cut(s) 375
BsrDI GCAATG 2 cut(s) 271, 783
BsrFI RCCGGY 2 cut(s) 204, 867
BsrI ACTGG 2 cut(s) 955, 2239
BssAI RCCGGY 2 cut(s) 204, 867
BssECI CCNNGG 5 cut(s) 472, 583, 1297, 2091, 2129
BssMI GATC 7 cut(s) 535, 1015, 1115, 1562, 1747, 1957, 2265
BssNI GRCGYC 1 cut(s) 1839
BssT1I CCWWGG 3 cut(s) 1297, 2091, 2129
Bst2UI CCWGG 3 cut(s) 129, 473, 873
Bst4CI ACNGT 6 cut(s) 302, 321, 607, 1050, 1686, 2023
Bst6I CTCTTC 3 cut(s) 1908, 1947, 2151
BstACI GRCGYC 1 cut(s) 1839
BstAPI GCANNNNNTGC 1 cut(s) 28
BstC8I GCNNGC 5 cut(s) 120, 190, 814, 869, 2261
BstDEI CTNAG 8 cut(s) 114, 1062, 1109, 1636, 1792, 1908, 2163, 2188
BstDSI CCRYGG 2 cut(s) 2091, 2129
BstEII GGTNACC 1 cut(s) 467
BstF5I GGATG 7 cut(s) 24, 446, 569, 1131, 1696, 2125, 2212
BstFNI CGCG 1 cut(s) 668
BstHHI GCGC 1 cut(s) 457
BstKTI GATC 7 cut(s) 538, 1018, 1118, 1565, 1750, 1960, 2268
BstMAI GTCTC 5 cut(s) 396, 1071, 1223, 2003, 2153
BstMBI GATC 7 cut(s) 535, 1015, 1115, 1562, 1747, 1957, 2265
BstMCI CGRYCG 1 cut(s) 1052
BstMWI GCNNNNNNNGC 8 cut(s) 28, 124, 189, 818, 1402, 1607, 2033, 2269
BstNI CCWGG 3 cut(s) 129, 473, 873
BstNSI RCATGY 3 cut(s) 427, 816, 2263
BstPAI GACNNNNGTC 1 cut(s) 1048
BstPI GGTNACC 1 cut(s) 467
BstSCI CCNGG 5 cut(s) 127, 471, 583, 734, 871
BstSFI CTRYAG 3 cut(s) 317, 943, 1843
BstUI CGCG 1 cut(s) 668
BstV1I GCAGC 7 cut(s) 119, 338, 672, 800, 1352, 1610, 2074
BstV2I GAAGAC 3 cut(s) 690, 704, 837
BstX2I RGATCY 2 cut(s) 1115, 2265
BstXI CCANNNNNNTGG 1 cut(s) 2098
BstYI RGATCY 2 cut(s) 1115, 2265
Bsu36I CCTNAGG 1 cut(s) 1908
BsuI GTATCC 2 cut(s) 538, 1781
BsuRI GGCC 5 cut(s) 127, 871, 1906, 2027, 2071
BtgI CCRYGG 2 cut(s) 2091, 2129
BtsCI GGATG 7 cut(s) 24, 446, 569, 1131, 1696, 2125, 2212
BtsIMutI CAGTG 1 cut(s) 2028
BveI ACCTGC 2 cut(s) 839, 965
Cac8I GCNNGC 5 cut(s) 120, 190, 814, 869, 2261
CfoI GCGC 1 cut(s) 457
Cfr10I RCCGGY 2 cut(s) 204, 867
Cfr13I GGNCC 2 cut(s) 360, 2225
Csp6I GTAC 8 cut(s) 610, 705, 948, 1003, 1165, 1779, 2232, 2238
CspAI ACCGGT 1 cut(s) 204
CspCI CAANNNNNGTGG 2 cut(s) 1866, 1901
CviAII CATG 7 cut(s) 424, 813, 1283, 1544, 2092, 2130, 2260
CviQI GTAC 8 cut(s) 610, 705, 948, 1003, 1165, 1779, 2232, 2238
DdeI CTNAG 8 cut(s) 114, 1062, 1109, 1636, 1792, 1908, 2163, 2188
DpnI GATC 7 cut(s) 537, 1017, 1117, 1564, 1749, 1959, 2267
DpnII GATC 7 cut(s) 535, 1015, 1115, 1562, 1747, 1957, 2265
DraI TTTAAA 2 cut(s) 463, 1171
EaeI YGGCCR 2 cut(s) 869, 2025
Eam1104I CTCTTC 3 cut(s) 1908, 1947, 2151
EarI CTCTTC 3 cut(s) 1908, 1947, 2151
EciI GGCGGA 1 cut(s) 99
Ecl136II GAGCTC 2 cut(s) 307, 382
Eco130I CCWWGG 3 cut(s) 1297, 2091, 2129
Eco147I AGGCCT 1 cut(s) 1906
Eco24I GRGCYC 2 cut(s) 309, 384
Eco47I GGWCC 2 cut(s) 360, 2225
Eco53kI GAGCTC 2 cut(s) 307, 382
Eco57I CTGAAG 1 cut(s) 831
Eco81I CCTNAGG 1 cut(s) 1908
Eco91I GGTNACC 1 cut(s) 467
EcoICRI GAGCTC 2 cut(s) 307, 382
EcoO65I GGTNACC 1 cut(s) 467
EcoRII CCWGG 3 cut(s) 127, 471, 871
EcoT14I CCWWGG 3 cut(s) 1297, 2091, 2129
EcoT22I ATGCAT 1 cut(s) 24
EcoT38I GRGCYC 2 cut(s) 309, 384
ErhI CCWWGG 3 cut(s) 1297, 2091, 2129
FaeI CATG 7 cut(s) 427, 816, 1286, 1547, 2095, 2133, 2263
FalI AAGNNNNNCTT 8 cut(s) 967, 999, 1102, 1134, 1743, 1775, 1875, 1907
FaqI GGGAC 2 cut(s) 616, 659
FatI CATG 7 cut(s) 423, 812, 1282, 1543, 2091, 2129, 2259
FauI CCCGC 1 cut(s) 964
FauNDI CATATG 4 cut(s) 451, 509, 857, 2284
FbaI TGATCA 1 cut(s) 1747
Fnu4HI GCNGC 7 cut(s) 133, 327, 661, 789, 1341, 1599, 2088
FokI GGATG 7 cut(s) 31, 453, 576, 1138, 1703, 2112, 2219
FriOI GRGCYC 2 cut(s) 309, 384
Fsp4HI GCNGC 7 cut(s) 133, 327, 661, 789, 1341, 1599, 2088
FspBI CTAG 9 cut(s) 71, 495, 1086, 1209, 1499, 1619, 1925, 2033, 2229
GlaI GCGC 1 cut(s) 456
GluI GCNGC 7 cut(s) 133, 327, 661, 789, 1341, 1599, 2088
GsaI CCCAGC 1 cut(s) 1759
GsuI CTGGAG 2 cut(s) 918, 1632
HaeIII GGCC 5 cut(s) 127, 871, 1906, 2027, 2071
HapII CCGG 5 cut(s) 205, 585, 736, 743, 868
HhaI GCGC 1 cut(s) 457
Hin1I GRCGYC 1 cut(s) 1839
Hin1II CATG 7 cut(s) 427, 816, 1286, 1547, 2095, 2133, 2263
Hin6I GCGC 1 cut(s) 455
HinP1I GCGC 1 cut(s) 455
HincII GTYRAC 3 cut(s) 258, 1414, 2185
HindII GTYRAC 3 cut(s) 258, 1414, 2185
HindIII AAGCTT 1 cut(s) 619
HinfI GANTC 7 cut(s) 647, 938, 1293, 1587, 1738, 1816, 2112
HpaI GTTAAC 1 cut(s) 2185
HpaII CCGG 5 cut(s) 205, 585, 736, 743, 868
HphI GGTGA 3 cut(s) 314, 1313, 1468
Hpy166II GTNNAC 6 cut(s) 258, 610, 1324, 1414, 1504, 2185
Hpy188I TCNGA 7 cut(s) 379, 996, 1644, 1717, 1787, 2155, 2191
Hpy8I GTNNAC 6 cut(s) 258, 610, 1324, 1414, 1504, 2185
Hpy99I CGWCG 1 cut(s) 1050
HpyAV CCTTC 8 cut(s) 116, 875, 1208, 1258, 1298, 1393, 2056, 2096
HpyCH4III ACNGT 6 cut(s) 302, 321, 607, 1050, 1686, 2023
HpyCH4IV ACGT 2 cut(s) 250, 1839
HpyF10VI GCNNNNNNNGC 8 cut(s) 28, 124, 189, 818, 1402, 1607, 2033, 2269
HpyF3I CTNAG 8 cut(s) 114, 1062, 1109, 1636, 1792, 1908, 2163, 2188
HpySE526I ACGT 2 cut(s) 250, 1839
Hsp92I GRCGYC 1 cut(s) 1839
Hsp92II CATG 7 cut(s) 427, 816, 1286, 1547, 2095, 2133, 2263
HspAI GCGC 1 cut(s) 455
KroI GCCGGC 1 cut(s) 867
KroNI GCCGGC 1 cut(s) 869
Ksp22I TGATCA 1 cut(s) 1747
KspAI GTTAAC 1 cut(s) 2185
Kzo9I GATC 7 cut(s) 535, 1015, 1115, 1562, 1747, 1957, 2265
LmnI GCTCC 8 cut(s) 312, 380, 937, 1402, 1613, 1724, 1867, 2095
Lsp1109I GCAGC 7 cut(s) 119, 338, 672, 800, 1352, 1610, 2074
LweI GCATC 9 cut(s) 9, 40, 334, 431, 647, 1045, 1635, 2134, 2182
MaeI CTAG 9 cut(s) 71, 495, 1086, 1209, 1499, 1619, 1925, 2033, 2229
MaeII ACGT 2 cut(s) 250, 1839
MalI GATC 7 cut(s) 537, 1017, 1117, 1564, 1749, 1959, 2267
MbiI CCGCTC 1 cut(s) 375
MboI GATC 7 cut(s) 535, 1015, 1115, 1562, 1747, 1957, 2265
MflI RGATCY 2 cut(s) 1115, 2265
MhlI GDGCHC 5 cut(s) 309, 384, 806, 1539, 1618
MlsI TGGCCA 1 cut(s) 2027
MluI ACGCGT 1 cut(s) 666
MluNI TGGCCA 1 cut(s) 2027
MlyI GAGTC 3 cut(s) 641, 932, 1581
MmeI TCCRAC 7 cut(s) 104, 110, 418, 522, 541, 675, 1668
Mox20I TGGCCA 1 cut(s) 2027
Mph1103I ATGCAT 1 cut(s) 24
MroNI GCCGGC 1 cut(s) 867
MscI TGGCCA 1 cut(s) 2027
MseI TTAA 6 cut(s) 462, 1170, 1946, 2054, 2184, 2276
MslI CAYNNNNRTG 3 cut(s) 1449, 1542, 1662
Msp20I TGGCCA 1 cut(s) 2027
MspA1I CMGCKG 4 cut(s) 118, 971, 1601, 2272
MspI CCGG 5 cut(s) 205, 585, 736, 743, 868
MspR9I CCNGG 5 cut(s) 129, 473, 585, 736, 873
Mva1269I GAATGC 1 cut(s) 1143
MvaI CCWGG 3 cut(s) 129, 473, 873
MvnI CGCG 1 cut(s) 668
MwoI GCNNNNNNNGC 8 cut(s) 28, 124, 189, 818, 1402, 1607, 2033, 2269
NaeI GCCGGC 1 cut(s) 869
NciI CCSGG 2 cut(s) 585, 736
NcoI CCATGG 2 cut(s) 2091, 2129
NdeI CATATG 4 cut(s) 451, 509, 857, 2284
NdeII GATC 7 cut(s) 535, 1015, 1115, 1562, 1747, 1957, 2265
NgoMIV GCCGGC 1 cut(s) 867
NlaIII CATG 7 cut(s) 427, 816, 1286, 1547, 2095, 2133, 2263
NlaIV GGNNCC 4 cut(s) 866, 887, 933, 955
NmeAIII GCCGAG 1 cut(s) 935
NmuCI GTSAC 4 cut(s) 221, 512, 779, 1835
NsiI ATGCAT 1 cut(s) 24
NspI RCATGY 3 cut(s) 427, 816, 2263
PaeI GCATGC 2 cut(s) 816, 2263
PaqCI CACCTGC 1 cut(s) 839
PceI AGGCCT 1 cut(s) 1906
PciI ACATGT 1 cut(s) 423
PctI GAATGC 1 cut(s) 1143
PdiI GCCGGC 1 cut(s) 869
PfeI GAWTC 4 cut(s) 1293, 1738, 1816, 2112
PinAI ACCGGT 1 cut(s) 204
PkrI GCNGC 7 cut(s) 134, 328, 662, 790, 1342, 1600, 2089
PleI GAGTC 3 cut(s) 641, 932, 1581
PpsI GAGTC 3 cut(s) 641, 932, 1581
PscI ACATGT 1 cut(s) 423
PshAI GACNNNNGTC 1 cut(s) 1048
Psp124BI GAGCTC 2 cut(s) 309, 384
Psp6I CCWGG 3 cut(s) 127, 471, 871
PspEI GGTNACC 1 cut(s) 467
PspFI CCCAGC 1 cut(s) 1755
PspGI CCWGG 3 cut(s) 127, 471, 871
PspN4I GGNNCC 4 cut(s) 866, 887, 933, 955
PspPI GGNCC 2 cut(s) 360, 2225
PstI CTGCAG 1 cut(s) 947
PsuI RGATCY 2 cut(s) 1115, 2265
PvuII CAGCTG 3 cut(s) 118, 1601, 2272
RsaI GTAC 8 cut(s) 611, 706, 949, 1004, 1166, 1780, 2233, 2239
RsaNI GTAC 8 cut(s) 610, 705, 948, 1003, 1165, 1779, 2232, 2238
RseI CAYNNNNRTG 3 cut(s) 1449, 1542, 1662
SacI GAGCTC 2 cut(s) 309, 384
SaqAI TTAA 6 cut(s) 462, 1170, 1946, 2054, 2184, 2276
SatI GCNGC 7 cut(s) 133, 327, 661, 789, 1341, 1599, 2088
Sau3AI GATC 7 cut(s) 535, 1015, 1115, 1562, 1747, 1957, 2265
Sau96I GGNCC 2 cut(s) 360, 2225
ScaI AGTACT 3 cut(s) 949, 1166, 2233
SchI GAGTC 3 cut(s) 641, 932, 1581
ScrFI CCNGG 5 cut(s) 129, 473, 585, 736, 873
SduI GDGCHC 5 cut(s) 309, 384, 806, 1539, 1618
SfaNI GCATC 9 cut(s) 9, 40, 334, 431, 647, 1045, 1635, 2134, 2182
SfcI CTRYAG 3 cut(s) 317, 943, 1843
SinI GGWCC 2 cut(s) 360, 2225
SmiMI CAYNNNNRTG 3 cut(s) 1449, 1542, 1662
SmlI CTYRAG 3 cut(s) 348, 1259, 1589
SmoI CTYRAG 3 cut(s) 348, 1259, 1589
SpeI ACTAGT 2 cut(s) 1085, 1498
SphI GCATGC 2 cut(s) 816, 2263
SseBI AGGCCT 1 cut(s) 1906
SsiI CCGC 5 cut(s) 110, 239, 373, 434, 971
SspI AATATT 1 cut(s) 1335
SspMI CTAG 9 cut(s) 71, 495, 1086, 1209, 1499, 1619, 1925, 2033, 2229
SstI GAGCTC 2 cut(s) 309, 384
StuI AGGCCT 1 cut(s) 1906
StyD4I CCNGG 5 cut(s) 127, 471, 583, 734, 871
StyI CCWWGG 3 cut(s) 1297, 2091, 2129
TaaI ACNGT 6 cut(s) 302, 321, 607, 1050, 1686, 2023
TaiI ACGT 2 cut(s) 253, 1842
TaqI TCGA 6 cut(s) 198, 715, 1052, 1660, 1977, 2007
TaqII GACCGA 2 cut(s) 377, 831
TatI WGTACW 5 cut(s) 609, 704, 947, 1164, 2231
TfiI GAWTC 4 cut(s) 1293, 1738, 1816, 2112
Tru1I TTAA 6 cut(s) 462, 1170, 1946, 2054, 2184, 2276
Tru9I TTAA 6 cut(s) 462, 1170, 1946, 2054, 2184, 2276
TscAI CASTG 1 cut(s) 2028
TseFI GTSAC 4 cut(s) 221, 512, 779, 1835
TseI GCWGC 7 cut(s) 132, 326, 660, 788, 1340, 1598, 2087
Tsp45I GTSAC 4 cut(s) 221, 512, 779, 1835
TspDTI ATGAA 5 cut(s) 1299, 1563, 1707, 1983, 2093
TspRI CASTG 1 cut(s) 2028
VpaK11BI GGWCC 2 cut(s) 360, 2225
XapI RAATTY 4 cut(s) 1239, 1380, 1971, 2145
XceI RCATGY 3 cut(s) 427, 816, 2263
XspI CTAG 9 cut(s) 71, 495, 1086, 1209, 1499, 1619, 1925, 2033, 2229
ZraI GACGTC 1 cut(s) 1840
ZrmI AGTACT 3 cut(s) 949, 1166, 2233
Zsp2I ATGCAT 1 cut(s) 24
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.