MD10G1251400.v1.1

specification of plant organ axis polarity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000066
Physical Location & Seq
Forward (+)
34469819 .. 34474944
5126 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1251400.v1.1.491

Sequence Viewer

Length: 2286 bp
ATGGCGTTACATTGGAGGATGCATATTTTGCATCTCTCTTTAGTAATGGTGGTAGTTGTTACAATTTTACTAGCAACTCCAACAACAGCTCAATCTCAACTGGCACTGCCTGACTGCCCTGACCGTTGTGGTAATCTCACAATTCCATACCCATTTGGCATAGGCTCTGGTTGCTACCTCGACGACCGGTTTGAAATCACTTGTAACATTTCCACTCAACCGCCAACAGCATTTATGATGACGGGCAATCTCGAAATTACCAACATATCTCTTTATGACGGTGAGCTCCAAATACTACAGTACGTGGCCAGTGATTGTTATGATGCTCAAGGCAACACGATCGAATGGTACAGTCCCTGGTTCACGGTGCCTCCTCCTTACACCATCTCCCATACCAAAAACACGTTCGTTGCACTTGGATGCGACACATATTCGTTTTTTATAGGTTACCGGGGTGAAGAAAAGTACACAACTGGGTGTACGTCCATATGTAACAATCTTGGCAACGCTATCGATCAGCAGGATACTTGCTCTGGCGTTGGATGTTGCCAAACTAAAATCCCCAGTGGGCTGCAAAATCAGACTCTGGAGTTGTATAGCTATTACAATCATTCGGATGTGTGGGAATTCAACCCATGCAGCTACGCGTTCCTTGTGCAAGAAGGCGAGTTCAACTTTAACAGCACGAGTTTTCGAGAACTAAACTATACAACCCAGGCTCCGGCAATTATTGATTGGGAAATTGGGAATGAGTCATGTGATATTGCAGCCAAAAACGAGGGCACATTTGGATGCAAGGCAAATAGCTCCTGCCATAATCGGTCTTCAGGCTACATATGCCGGTGCCTGCCAGGTTACGAAGGGAATCCATACCTCCCGGATGGTTGCCAAGATATTGATGAGTGCATGGCTCCAAACCCCTGCAGTATGGGAACCTGCATAAATACACCAGGAAATTATACTTGCAACTGTCCGAAAGGGTACAAAAATGATGATCCGGATAAAATAAGATGCATCAAGGACAATGGTCGAGCAAAGCTCAGTCTCCTGCTTATTATCTCACTAGGTGCCAGTGGAGGCCTCTTGCTTTTACTAAGTGGATCTTGGATATATTGGGGAATGCAGAGAAGAAAGTTCCTCAAACTCAAAGAAAAATACTTTAAAGAAAATGGTGGCGTATTGTTACAACAAAAAATCGCTAGTCAAGGAGGCTCTGTGGAGACAACAAAAATTTTCACGGCAGAAGAACTTGAGAAGGCAACAAACAATTACCATGAAAGTAGAATCCTTGGTGAAGGAGGCTATGGAATAGTTTACAAAGGAATATTAGCAGCAGATAACAATAAAGCGTTTGCCATAAAAAAGTCTAAAATTGGTGTCCCAACGCAGAAGGAGCAGTTTGTCAACGAGTTGCTTGTTCTTTCTCAAATCAACCATAGAAATGTGGTGAGGTTATTGGGTTGTTGTTTGGAGTTAGAAGTTCCTCTACTAGTTTACGAGTTTGTCGCTCACGGCACTCTCTTTGAGCACATACATGGCAAAAAGAGGAAAGGATCATCATTTTCATTGGAATTACGACTCAAGATAGCAGCTGAAACTGCTGGAGCACTAGCATACTTACACTCCTCAGCTCTGATGCAAATCATACATCGAGATGTGAAACTGACAAATATACTGTTGGATGAAAATTACACGGCAAAAGTGTCAGACTTTGGAGCTTCACGATTGATTCCTCTTGATCAAGCCCAGCTTGCAACTTTAGTGCAAGGAACAATCGGATACTTAGACCCTGAATACTTCCACACGAGTCAACTAACAGATAAGAGTGACGTCTATAGCTTTGGAGTTGTCCTTATGGAGCTACTAACAAGTAAATTGGCACTTGATTCTCATAGGCCTGAGGGAGAGAAAAACCTGGCAAGGTTCTTTCTTTGTTTAATGGAAGAGGATCGCCTGAATGAAATTCTCGATGATGACATACTTAACGAGAGAAACATCGAGACAATAAAAACAGTGGCCAATCTGGCAAAAAGATGTGTAAGGTTAAAAGGGGAGGACAGGCCAACAATGAAAGAAGTTGCCATGGAGCTTGAAGGAATGAGAATTACAGAAAAGCATGCATGGGGAAAAGCTGAAATTTGTTCAGAAGAGACTGAGTGCTTACTTGGGCCAGGTAACTCAGATGCTTATCCTGTGGATGTTAGAGCTGGTTCTAGTACTGGTACAACTATTGTGTATGACAGCATGCAGATCCAGATGTTAATTCCATATGATGATGGAAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind Pkinase_Tyr

Protein Analysis

762

Amino Acids

84.66

Weight (kDa)

5.24

Isoelectric Point (pI)

32.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 39 - 98 1.2e-11 Wall-associated receptor kinase galacturonan-binding
EGF_CA PF07645 298 - 334 1e-07 Calcium-binding EGF domain
EGF PF00008 305 - 330 6e-06 EGF-like domain
Pkinase PF00069 424 - 693 3e-46 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 427 - 697 5.1e-46 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21210 AT1G21230 AT1G21240 AT1G21240 AT1G21245 AT1G21250 AT1G21270 AT1G22720 AT3G25490
fragaria_vesca FvH4_2g35051 FvH4_2g35051 FvH4_2g35060 FvH4_3g10281 FvH4_3g10285 FvH4_3g10286 FvH4_3g10311 FvH4_3g10311 FvH4_3g10320 FvH4_3g10332 FvH4_3g10340 FvH4_3g10350 FvH4_3g10360 FvH4_3g10390 FvH4_3g10412 FvH4_3g10413 FvH4_3g10461 FvH4_3g10462 FvH4_3g10470 FvH4_3g10510 FvH4_3g10520 FvH4_3g10530 FvH4_3g10550 FvH4_3g10560 FvH4_3g10611 FvH4_3g11730 FvH4_6g53140 FvH4_6g53142 FvH4_7g31880 FvH4_7g31910 FvH4_7g31920
malus_domestica MD05G1269900.v1.1 MD05G1270000.v1.1 MD05G1270200.v1.1 MD05G1270400.v1.1 MD05G1270500.v1.1 MD05G1270600.v1.1 MD05G1270800.v1.1 MD05G1270900.v1.1 MD07G1183800.v1.1 MD08G1107600.v1.1 MD10G1248500.v1.1 MD10G1249200.v1.1 MD10G1249500.v1.1 MD10G1250000.v1.1 MD10G1250500.v1.1 MD10G1250900.v1.1 MD10G1251200.v1.1 MD10G1251400.v1.1 MD13G1004600.v1.1 MD15G1088400.v1.1
prunus_persica Prupe.1G442400_v2.0.a1 Prupe.1G442500_v2.0.a1 Prupe.2G223400_v2.0.a1 Prupe.4G091900_v2.0.a1 Prupe.4G093200_v2.0.a1 Prupe.4G093300_v2.0.a1 Prupe.4G093400_v2.0.a1 Prupe.4G093500_v2.0.a1 Prupe.4G094100_v2.0.a1 Prupe.4G094200_v2.0.a1 Prupe.4G094300_v2.0.a1 Prupe.6G358300_v2.0.a1
pyrus_communis pycom08g08990 pycom10g20660 pycom10g20670 pycom10g20680 pycom10g20710 pycom10g20740 pycom13g00400 pycom15g08290
rosa_chinensis RchiOBHm_Chr1g0326861 RchiOBHm_Chr1g0326871 RchiOBHm_Chr1g0331391 RchiOBHm_Chr1g0336911 RchiOBHm_Chr1g0364001 RchiOBHm_Chr2g0174541 RchiOBHm_Chr2g0175041 RchiOBHm_Chr2g0175101 RchiOBHm_Chr2g0175121 RchiOBHm_Chr3g0486331 RchiOBHm_Chr5g0015581 RchiOBHm_Chr5g0016551 RchiOBHm_Chr5g0016561 RchiOBHm_Chr5g0016581 RchiOBHm_Chr5g0016601 RchiOBHm_Chr5g0016611 RchiOBHm_Chr5g0016641 RchiOBHm_Chr5g0016661 RchiOBHm_Chr5g0016691 RchiOBHm_Chr5g0016701 RchiOBHm_Chr5g0016731 RchiOBHm_Chr5g0016741 RchiOBHm_Chr5g0016811 RchiOBHm_Chr5g0016841 RchiOBHm_Chr5g0016871 RchiOBHm_Chr5g0016881 RchiOBHm_Chr5g0016931 RchiOBHm_Chr5g0017021 RchiOBHm_Chr5g0017041 RchiOBHm_Chr5g0017071 RchiOBHm_Chr5g0017091 RchiOBHm_Chr5g0017121 RchiOBHm_Chr5g0017141 RchiOBHm_Chr5g0017291 RchiOBHm_Chr5g0017321 RchiOBHm_Chr5g0017401 RchiOBHm_Chr5g0019001 RchiOBHm_Chr5g0019071 RchiOBHm_Chr5g0028941 RchiOBHm_Chr5g0052141 RchiOBHm_Chr5g0054721 RchiOBHm_Chr5g0054801 RchiOBHm_Chr5g0054931 RchiOBHm_Chr5g0054941 RchiOBHm_Chr6g0259291 RchiOBHm_Chr6g0292991 RchiOBHm_Chr6g0306911
rosa_laevigata RLG00000009465 RLG00000009468 RLG00000010755 RLG00000011976 RLG00000014541 RLG00000014542 RLG00000015169 RLG00000022308 RLG00000027529 RLG00000029324 RLG00000030244 RLG00000030245 RLG00000032210 RLG00000032211 RLG00000032257 RLG00000032259 RLG00000032260 RLG00000032261 RLG00000032262 RLG00000032268 RLG00000032269 RLG00000032272 RLG00000032282 RLG00000032283 RLG00000032308 RLG00000032413 RLG00000033129 RLG00000034945
rosa_multiflora Rmu_co8241929.1_g000001 Rmu_co8269227.1_g000001 Rmu_co8461259.1_g000001 Rmu_co8495385.1_g000001 Rmu_sc0000028.1_g000003 Rmu_sc0000083.1_g000009 Rmu_sc0000083.1_g000010 Rmu_sc0000083.1_g000031 Rmu_sc0000083.1_g000043 Rmu_sc0000083.1_g000046 Rmu_sc0000083.1_g000047 Rmu_sc0000563.1_g000024 Rmu_sc0000842.1_g000012 Rmu_sc0001235.1_g000001 Rmu_sc0001235.1_g000005 Rmu_sc0001235.1_g000009 Rmu_sc0001235.1_g000015 Rmu_sc0001235.1_g000016 Rmu_sc0001235.1_g000017 Rmu_sc0001473.1_g000036 Rmu_sc0001607.1_g000016 Rmu_sc0001788.1_g000006 Rmu_sc0002018.1_g000008 Rmu_sc0002028.1_g000001 Rmu_sc0002680.1_g000011 Rmu_sc0002798.1_g000023 Rmu_sc0002886.1_g000023 Rmu_sc0002989.1_g000012 Rmu_sc0003064.1_g000009 Rmu_sc0003382.1_g000006 Rmu_sc0003630.1_g000002 Rmu_sc0003630.1_g000047 Rmu_sc0003630.1_g000066 Rmu_sc0003630.1_g000067 Rmu_sc0003649.1_g000010 Rmu_sc0003688.1_g000005 Rmu_sc0003920.1_g000007 Rmu_sc0004765.1_g000007 Rmu_sc0004765.1_g000008 Rmu_sc0004920.1_g000011 Rmu_sc0005223.1_g000008 Rmu_sc0005223.1_g000010 Rmu_sc0005223.1_g000015 Rmu_sc0005223.1_g000016 Rmu_sc0005319.1_g000014 Rmu_sc0005319.1_g000015 Rmu_sc0005319.1_g000018 Rmu_sc0005319.1_g000019 Rmu_sc0005319.1_g000025 Rmu_sc0005418.1_g000007 Rmu_sc0005418.1_g000012 Rmu_sc0006106.1_g000011 Rmu_sc0006914.1_g000012 Rmu_sc0007023.1_g000001 Rmu_sc0007662.1_g000001 Rmu_sc0007920.1_g000002 Rmu_sc0007920.1_g000013 Rmu_sc0008053.1_g000016 Rmu_sc0008053.1_g000017 Rmu_sc0008053.1_g000018 Rmu_sc0008053.1_g000023 Rmu_sc0008053.1_g000050 Rmu_sc0009042.1_g000007 Rmu_sc0009042.1_g000008 Rmu_sc0010642.1_g000008 Rmu_sc0011109.1_g000002 Rmu_sc0011314.1_g000006 Rmu_sc0011974.1_g000006 Rmu_sc0012179.1_g000009 Rmu_sc0015169.1_g000001 Rmu_sc0016368.1_g000002 Rmu_sc0022659.1_g000001 Rmu_sc0027371.1_g000001 Rmu_sc0028213.1_g000001 Rmu_sc0034526.1_g000001 Rmu_sc0039366.1_g000002 Rmu_ssc0000050.1_g000084 Rmu_ssc0000050.1_g000088 Rmu_ssc0000167.1_g000017
rosa_roxburghii Rroxscaffold_1G00011340 Rroxscaffold_1G00025710 Rroxscaffold_1G00025870 Rroxscaffold_1G00026070 Rroxscaffold_1G00026170 Rroxscaffold_1G00026200 Rroxscaffold_1G00026270 Rroxscaffold_1G00050950 Rroxscaffold_1G00058900 Rroxscaffold_1G00060040 Rroxscaffold_1G00060060 Rroxscaffold_1G00060070 Rroxscaffold_1G00060080 Rroxscaffold_1G00060110 Rroxscaffold_1G00060120 Rroxscaffold_1G00060140 Rroxscaffold_1G00060170 Rroxscaffold_1G00060210 Rroxscaffold_1G00060220 Rroxscaffold_1G00060240 Rroxscaffold_1G00060250 Rroxscaffold_1G00060260 Rroxscaffold_1G00060300 Rroxscaffold_1G00060320 Rroxscaffold_1G00060330 Rroxscaffold_1G00060900 Rroxscaffold_1G00060920 Rroxscaffold_1G00073980 Rroxscaffold_2G00077560 Rroxscaffold_2G00077580 Rroxscaffold_2G00146990 Rroxscaffold_3G00236200 Rroxscaffold_3G00236210 Rroxscaffold_3G00236220 Rroxscaffold_4G00292760 Rroxscaffold_4G00315200 Rroxscaffold_4G00315240 Rroxscaffold_4G00326240 Rroxscaffold_4G00328980 Rroxscaffold_5G00341890 Rroxscaffold_7G00161350 Rroxscaffold_7G00174490 Rroxscaffold_7G00206550
rosa_rugosa Rorug01G0093000 Rorug01G0093100 Rorug01G0128800 Rorug01G0312000 Rorug01G0312000 Rorug02G0581100 Rorug03G0224600 Rorug05G0028500 Rorug05G0032700 Rorug05G0032800 Rorug05G0032900 Rorug05G0032900 Rorug05G0033000 Rorug05G0033100 Rorug05G0033300 Rorug05G0033400 Rorug05G0033900 Rorug05G0034900 Rorug05G0035000 Rorug05G0046900 Rorug05G0047500 Rorug05G0047500 Rorug05G0111600 Rorug05G0287900 Rorug05G0288700 Rorug05G0586900 Rorug06G0231700 Rorug06G0354300 Rorug06G0354400
rosa_samantha Rh1DG122200 Rh2DG686800 Rh5BG136600 Rh6AG344500 Rh6BG351900 Rh6CG359000 Rh6CG359100 Rh6CG387600 Rh6CG480500 Rh6DG344600 Rh6DG467400
rosa_wichuraiana Rw0G010250 Rw0G014820 Rw0G021400 Rw0G023100 Rw1G009450 Rw1G012100 Rw1G012180 Rw1G028360 Rw2G054260 Rw3G024370 Rw3G024420 Rw3G024470 Rw5G010850 Rw5G011010 Rw5G011020 Rw5G011050 Rw5G011080 Rw5G011120 Rw5G012300 Rw5G018470 Rw5G033830 Rw6G008930 Rw6G030070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1833
Acc36I ACCTGC 1 cut(s) 944
AccB1I GGYRCC 3 cut(s) 367, 843, 1067
AccII CGCG 1 cut(s) 647
AccIII TCCGGA 1 cut(s) 997
AciI CCGC 1 cut(s) 221
AclWI GGATC 5 cut(s) 989, 1108, 1561, 1956, 2245
AcoI YGGCCR 2 cut(s) 306, 2016
AcsI RAATTY 4 cut(s) 626, 1230, 1962, 2136
AcuI CTGAAG 1 cut(s) 810
AcyI GRCGYC 1 cut(s) 1830
AdeI CACNNNGTG 1 cut(s) 1067
AfaI GTAC 7 cut(s) 302, 350, 467, 481, 983, 2218, 2224
AfiI CCNNNNNNNGG 1 cut(s) 721
AflIII ACRYGT 2 cut(s) 402, 645
AgeI ACCGGT 1 cut(s) 186
AgsI TTSAA 4 cut(s) 194, 631, 673, 2093
AhlI ACTAGT 1 cut(s) 1489
AjnI CCWGG 6 cut(s) 356, 714, 850, 949, 1914, 2170
AjuI GAANNNNNNNTTGG 2 cut(s) 389, 421
Alw21I GWGCWC 3 cut(s) 288, 1530, 1609
Alw26I GTCTC 4 cut(s) 1049, 1214, 1994, 2144
AlwI GGATC 5 cut(s) 989, 1108, 1561, 1956, 2245
AlwNI CAGNNNCTG 2 cut(s) 357, 586
Aor13HI TCCGGA 1 cut(s) 997
AoxI GGCC 6 cut(s) 306, 1078, 1895, 2016, 2060, 2168
ApeKI GCWGC 5 cut(s) 571, 639, 767, 1331, 1589
ApoI RAATTY 4 cut(s) 626, 1230, 1962, 2136
ArsI GACNNNNNNTTYG 2 cut(s) 173, 205
AsiGI ACCGGT 1 cut(s) 186
AspS9I GGNCC 1 cut(s) 2168
AsuC2I CCSGG 2 cut(s) 452, 878
AsuHPI GGTGA 4 cut(s) 293, 467, 1304, 1459
AxyI CCTNAGG 1 cut(s) 1899
BaeGI GKGCMC 1 cut(s) 785
BalI TGGCCA 2 cut(s) 308, 2018
BanI GGYRCC 3 cut(s) 367, 843, 1067
BanII GRGCYC 1 cut(s) 288
BarI GAAGNNNNNNTAC 2 cut(s) 1470, 1502
BauI CACGAG 2 cut(s) 685, 1804
BbsI GAAGAC 1 cut(s) 816
Bbv12I GWGCWC 3 cut(s) 288, 1530, 1609
BbvCI CCTCAGC 1 cut(s) 1627
BbvI GCAGC 5 cut(s) 558, 651, 779, 1343, 1601
BccI CCATC 3 cut(s) 392, 875, 2270
BceAI ACGGC 3 cut(s) 1254, 1528, 1710
BcgI CGANNNNNNTGC 4 cut(s) 322, 356, 493, 527
BciT130I CCWGG 6 cut(s) 358, 716, 852, 951, 1916, 2172
BciVI GTATCC 2 cut(s) 517, 1772
BclI TGATCA 1 cut(s) 1738
BcnI CCSGG 2 cut(s) 452, 878
BcoDI GTCTC 4 cut(s) 1049, 1214, 1994, 2144
BcuI ACTAGT 1 cut(s) 1489
BfaI CTAG 6 cut(s) 71, 1064, 1200, 1490, 1610, 2214
BfmI CTRYAG 3 cut(s) 296, 922, 1834
BfuAI ACCTGC 1 cut(s) 944
BfuI GTATCC 2 cut(s) 517, 1772
BglI GCCNNNNNGGC 1 cut(s) 2024
BisI GCNGC 5 cut(s) 572, 640, 768, 1332, 1590
BlsI GCNGC 5 cut(s) 573, 641, 769, 1333, 1591
BmcAI AGTACT 1 cut(s) 2218
Bme1390I CCNGG 8 cut(s) 358, 452, 716, 852, 878, 951, 1916, 2172
BmgT120I GGNCC 1 cut(s) 2168
BmiI GGNNCC 6 cut(s) 369, 720, 845, 912, 934, 1069
BmrFI CCNGG 8 cut(s) 358, 452, 716, 852, 878, 951, 1916, 2172
BmrI ACTGGG 2 cut(s) 483, 558
BmsI GCATC 9 cut(s) 9, 40, 313, 410, 782, 1001, 1023, 1626, 2173
BmuI ACTGGG 2 cut(s) 483, 558
BoxI GACNNNNGTC 1 cut(s) 1026
BpiI GAAGAC 1 cut(s) 816
BplI GAGNNNNNCTC 2 cut(s) 1023, 1055
BpmI CTGGAG 2 cut(s) 608, 1623
Bpu10I CCTNAGC 1 cut(s) 1627
BpuEI CTTGAG 3 cut(s) 312, 1271, 1565
BpuMI CCSGG 2 cut(s) 452, 878
Bsa29I ATCGAT 1 cut(s) 513
BsaAI YACGTR 1 cut(s) 304
BsaBI GATNNNNATC 2 cut(s) 1640, 2187
BsaHI GRCGYC 1 cut(s) 1830
BsaJI CCNNGG 5 cut(s) 356, 451, 714, 1288, 2082
BsaWI WCCGGW 2 cut(s) 186, 997
Bsc4I CCNNNNNNNGG 1 cut(s) 721
Bse118I RCCGGY 2 cut(s) 186, 840
Bse1I ACTGG 6 cut(s) 105, 309, 478, 564, 1071, 2224
Bse21I CCTNAGG 1 cut(s) 1899
Bse8I GATNNNNATC 2 cut(s) 1640, 2187
BseAI TCCGGA 1 cut(s) 997
BseBI CCWGG 6 cut(s) 358, 716, 852, 951, 1916, 2172
BseCI ATCGAT 1 cut(s) 513
BseDI CCNNGG 5 cut(s) 356, 451, 714, 1288, 2082
BseGI GGATG 8 cut(s) 24, 425, 548, 622, 797, 886, 1687, 2203
BseJI GATNNNNATC 2 cut(s) 1640, 2187
BseLI CCNNNNNNNGG 1 cut(s) 721
BseMII CTCAG 5 cut(s) 1054, 1641, 1890, 2145, 2193
BseNI ACTGG 6 cut(s) 105, 309, 478, 564, 1071, 2224
BseRI GAGGAG 2 cut(s) 363, 1615
BseSI GKGCMC 1 cut(s) 785
BseXI GCAGC 5 cut(s) 558, 651, 779, 1343, 1601
BseYI CCCAGC 1 cut(s) 1746
Bsh1236I CGCG 1 cut(s) 647
Bsh1285I CGRYCG 2 cut(s) 187, 342
BshFI GGCC 6 cut(s) 308, 1080, 1897, 2018, 2062, 2170
BshNI GGYRCC 3 cut(s) 367, 843, 1067
BshTI ACCGGT 1 cut(s) 186
BshVI ATCGAT 1 cut(s) 513
BsiEI CGRYCG 2 cut(s) 187, 342
BsiHKAI GWGCWC 3 cut(s) 288, 1530, 1609
BsiSI CCGG 6 cut(s) 187, 451, 722, 841, 878, 998
BslFI GGGAC 2 cut(s) 339, 1364
BslI CCNNNNNNNGG 1 cut(s) 721
BsmAI GTCTC 4 cut(s) 1049, 1214, 1994, 2144
BsmFI GGGAC 2 cut(s) 339, 1364
BsmI GAATGC 1 cut(s) 1125
BsnI GGCC 6 cut(s) 308, 1080, 1897, 2018, 2062, 2170
Bsp1286I GDGCHC 4 cut(s) 288, 785, 1530, 1609
Bsp13I TCCGGA 1 cut(s) 997
Bsp143I GATC 8 cut(s) 339, 514, 994, 1100, 1553, 1738, 1948, 2250
Bsp19I CCATGG 1 cut(s) 2082
BspACI CCGC 1 cut(s) 221
BspANI GGCC 6 cut(s) 308, 1080, 1897, 2018, 2062, 2170
BspCNI CTCAG 5 cut(s) 1053, 1640, 1891, 2146, 2192
BspDI ATCGAT 1 cut(s) 513
BspEI TCCGGA 1 cut(s) 997
BspFNI CGCG 1 cut(s) 647
BspLI GGNNCC 6 cut(s) 369, 720, 845, 912, 934, 1069
BspMAI CTGCAG 1 cut(s) 926
BspMI ACCTGC 1 cut(s) 944
BspPI GGATC 5 cut(s) 989, 1108, 1561, 1956, 2245
BspT107I GGYRCC 3 cut(s) 367, 843, 1067
BsrFI RCCGGY 2 cut(s) 186, 840
BsrI ACTGG 6 cut(s) 105, 309, 478, 564, 1071, 2224
BssAI RCCGGY 2 cut(s) 186, 840
BssECI CCNNGG 5 cut(s) 356, 451, 714, 1288, 2082
BssMI GATC 8 cut(s) 339, 514, 994, 1100, 1553, 1738, 1948, 2250
BssNI GRCGYC 1 cut(s) 1830
BssSI CACGAG 2 cut(s) 685, 1804
BssT1I CCWWGG 2 cut(s) 1288, 2082
Bst2BI CACGAG 2 cut(s) 685, 1804
Bst2UI CCWGG 6 cut(s) 358, 716, 852, 951, 1916, 2172
Bst4CI ACNGT 8 cut(s) 125, 281, 300, 353, 367, 971, 1677, 2014
Bst6I CTCTTC 2 cut(s) 1938, 2142
BstACI GRCGYC 1 cut(s) 1830
BstAPI GCANNNNNTGC 1 cut(s) 28
BstBAI YACGTR 1 cut(s) 304
BstC8I GCNNGC 4 cut(s) 848, 1752, 2118, 2246
BstDEI CTNAG 7 cut(s) 1040, 1094, 1627, 1783, 1899, 2154, 2179
BstDSI CCRYGG 1 cut(s) 2082
BstEII GGTNACC 1 cut(s) 446
BstF5I GGATG 8 cut(s) 24, 425, 548, 622, 797, 886, 1687, 2203
BstFNI CGCG 1 cut(s) 647
BstKTI GATC 8 cut(s) 342, 517, 997, 1103, 1556, 1741, 1951, 2253
BstMAI GTCTC 4 cut(s) 1049, 1214, 1994, 2144
BstMBI GATC 8 cut(s) 339, 514, 994, 1100, 1553, 1738, 1948, 2250
BstMCI CGRYCG 2 cut(s) 187, 342
BstMWI GCNNNNNNNGC 7 cut(s) 28, 171, 837, 1393, 1598, 1751, 2024
BstNI CCWGG 6 cut(s) 358, 716, 852, 951, 1916, 2172
BstNSI RCATGY 2 cut(s) 2120, 2248
BstPAI GACNNNNGTC 1 cut(s) 1026
BstPI GGTNACC 1 cut(s) 446
BstSCI CCNGG 8 cut(s) 356, 450, 714, 850, 876, 949, 1914, 2170
BstSFI CTRYAG 3 cut(s) 296, 922, 1834
BstSLI GKGCMC 1 cut(s) 785
BstUI CGCG 1 cut(s) 647
BstV1I GCAGC 5 cut(s) 558, 651, 779, 1343, 1601
BstV2I GAAGAC 1 cut(s) 816
BstX2I RGATCY 2 cut(s) 1100, 2250
BstYI RGATCY 2 cut(s) 1100, 2250
Bsu15I ATCGAT 1 cut(s) 513
Bsu36I CCTNAGG 1 cut(s) 1899
BsuI GTATCC 2 cut(s) 517, 1772
BsuRI GGCC 6 cut(s) 308, 1080, 1897, 2018, 2062, 2170
BsuTUI ATCGAT 1 cut(s) 513
BtgI CCRYGG 1 cut(s) 2082
BtsCI GGATG 8 cut(s) 24, 425, 548, 622, 797, 886, 1687, 2203
BtsI GCAGTG 1 cut(s) 104
BtsIMutI CAGTG 5 cut(s) 104, 316, 571, 1078, 2019
BveI ACCTGC 1 cut(s) 944
Cac8I GCNNGC 4 cut(s) 848, 1752, 2118, 2246
CaiI CAGNNNCTG 2 cut(s) 357, 586
Cfr10I RCCGGY 2 cut(s) 186, 840
Cfr13I GGNCC 1 cut(s) 2168
ClaI ATCGAT 1 cut(s) 513
Csp6I GTAC 7 cut(s) 301, 349, 466, 480, 982, 2217, 2223
CspAI ACCGGT 1 cut(s) 186
CviAII CATG 9 cut(s) 636, 756, 907, 1274, 1535, 2083, 2117, 2121, 2245
CviQI GTAC 7 cut(s) 301, 349, 466, 480, 982, 2217, 2223
DdeI CTNAG 7 cut(s) 1040, 1094, 1627, 1783, 1899, 2154, 2179
DpnI GATC 8 cut(s) 341, 516, 996, 1102, 1555, 1740, 1950, 2252
DpnII GATC 8 cut(s) 339, 514, 994, 1100, 1553, 1738, 1948, 2250
DraI TTTAAA 1 cut(s) 1162
DraIII CACNNNGTG 1 cut(s) 1067
EaeI YGGCCR 2 cut(s) 306, 2016
Eam1104I CTCTTC 2 cut(s) 1938, 2142
EarI CTCTTC 2 cut(s) 1938, 2142
Ecl136II GAGCTC 1 cut(s) 286
Eco130I CCWWGG 2 cut(s) 1288, 2082
Eco147I AGGCCT 2 cut(s) 1080, 1897
Eco24I GRGCYC 1 cut(s) 288
Eco53kI GAGCTC 1 cut(s) 286
Eco57I CTGAAG 1 cut(s) 810
Eco81I CCTNAGG 1 cut(s) 1899
Eco91I GGTNACC 1 cut(s) 446
EcoICRI GAGCTC 1 cut(s) 286
EcoO65I GGTNACC 1 cut(s) 446
EcoRI GAATTC 1 cut(s) 626
EcoRII CCWGG 6 cut(s) 356, 714, 850, 949, 1914, 2170
EcoT14I CCWWGG 2 cut(s) 1288, 2082
EcoT22I ATGCAT 3 cut(s) 24, 1016, 2122
EcoT38I GRGCYC 1 cut(s) 288
ErhI CCWWGG 2 cut(s) 1288, 2082
FaeI CATG 9 cut(s) 639, 759, 910, 1277, 1538, 2086, 2120, 2124, 2248
FalI AAGNNNNNCTT 4 cut(s) 1087, 1119, 1734, 1766
FaqI GGGAC 2 cut(s) 339, 1364
FatI CATG 9 cut(s) 635, 755, 906, 1273, 1534, 2082, 2116, 2120, 2244
FauNDI CATATG 3 cut(s) 488, 836, 2269
FbaI TGATCA 1 cut(s) 1738
Fnu4HI GCNGC 5 cut(s) 572, 640, 768, 1332, 1590
FokI GGATG 8 cut(s) 31, 432, 555, 629, 804, 893, 1694, 2210
FriOI GRGCYC 1 cut(s) 288
Fsp4HI GCNGC 5 cut(s) 572, 640, 768, 1332, 1590
FspBI CTAG 6 cut(s) 71, 1064, 1200, 1490, 1610, 2214
GluI GCNGC 5 cut(s) 572, 640, 768, 1332, 1590
GsaI CCCAGC 1 cut(s) 1750
GsuI CTGGAG 2 cut(s) 608, 1623
HaeIII GGCC 6 cut(s) 308, 1080, 1897, 2018, 2062, 2170
HapII CCGG 6 cut(s) 187, 451, 722, 841, 878, 998
Hin1I GRCGYC 1 cut(s) 1830
Hin1II CATG 9 cut(s) 639, 759, 910, 1277, 1538, 2086, 2120, 2124, 2248
HincII GTYRAC 2 cut(s) 1405, 1811
HindII GTYRAC 2 cut(s) 1405, 1811
HinfI GANTC 8 cut(s) 583, 752, 865, 1284, 1578, 1729, 1807, 1886
HpaII CCGG 6 cut(s) 187, 451, 722, 841, 878, 998
HphI GGTGA 4 cut(s) 293, 467, 1304, 1459
Hpy166II GTNNAC 7 cut(s) 363, 468, 480, 1315, 1405, 1495, 1811
Hpy188I TCNGA 8 cut(s) 582, 616, 975, 1635, 1708, 1778, 2146, 2182
Hpy8I GTNNAC 7 cut(s) 363, 468, 480, 1315, 1405, 1495, 1811
Hpy99I CGWCG 1 cut(s) 185
HpyAV CCTTC 6 cut(s) 656, 854, 1249, 1289, 1384, 2087
HpyCH4III ACNGT 8 cut(s) 125, 281, 300, 353, 367, 971, 1677, 2014
HpyCH4IV ACGT 4 cut(s) 303, 404, 482, 1830
HpyF10VI GCNNNNNNNGC 7 cut(s) 28, 171, 837, 1393, 1598, 1751, 2024
HpyF3I CTNAG 7 cut(s) 1040, 1094, 1627, 1783, 1899, 2154, 2179
HpySE526I ACGT 4 cut(s) 303, 404, 482, 1830
Hsp92I GRCGYC 1 cut(s) 1830
Hsp92II CATG 9 cut(s) 639, 759, 910, 1277, 1538, 2086, 2120, 2124, 2248
Kpn2I TCCGGA 1 cut(s) 997
Ksp22I TGATCA 1 cut(s) 1738
Kzo9I GATC 8 cut(s) 339, 514, 994, 1100, 1553, 1738, 1948, 2250
LmnI GCTCC 9 cut(s) 291, 724, 812, 916, 1393, 1604, 1715, 1858, 2086
Lsp1109I GCAGC 5 cut(s) 558, 651, 779, 1343, 1601
LweI GCATC 9 cut(s) 9, 40, 313, 410, 782, 1001, 1023, 1626, 2173
MaeI CTAG 6 cut(s) 71, 1064, 1200, 1490, 1610, 2214
MaeII ACGT 4 cut(s) 303, 404, 482, 1830
MaeIII GTNAC 9 cut(s) 6, 58, 203, 446, 491, 854, 1182, 1826, 2174
MalI GATC 8 cut(s) 341, 516, 996, 1102, 1555, 1740, 1950, 2252
MboI GATC 8 cut(s) 339, 514, 994, 1100, 1553, 1738, 1948, 2250
MboII GAAGA 6 cut(s) 470, 816, 1140, 1256, 1955, 2159
MflI RGATCY 2 cut(s) 1100, 2250
MhlI GDGCHC 4 cut(s) 288, 785, 1530, 1609
MlsI TGGCCA 2 cut(s) 308, 2018
MluI ACGCGT 1 cut(s) 645
MluNI TGGCCA 2 cut(s) 308, 2018
MlyI GAGTC 4 cut(s) 577, 761, 1572, 1816
MmeI TCCRAC 3 cut(s) 104, 520, 1659
Mox20I TGGCCA 2 cut(s) 308, 2018
Mph1103I ATGCAT 3 cut(s) 24, 1016, 2122
MroI TCCGGA 1 cut(s) 997
MscI TGGCCA 2 cut(s) 308, 2018
MseI TTAA 6 cut(s) 678, 1161, 1937, 1983, 2045, 2261
MslI CAYNNNNRTG 6 cut(s) 418, 615, 790, 1440, 1533, 1653
Msp20I TGGCCA 2 cut(s) 308, 2018
MspA1I CMGCKG 1 cut(s) 1592
MspI CCGG 6 cut(s) 187, 451, 722, 841, 878, 998
MspR9I CCNGG 8 cut(s) 358, 452, 716, 852, 878, 951, 1916, 2172
Mva1269I GAATGC 1 cut(s) 1125
MvaI CCWGG 6 cut(s) 358, 716, 852, 951, 1916, 2172
MvnI CGCG 1 cut(s) 647
MwoI GCNNNNNNNGC 7 cut(s) 28, 171, 837, 1393, 1598, 1751, 2024
NciI CCSGG 2 cut(s) 452, 878
NcoI CCATGG 1 cut(s) 2082
NdeI CATATG 3 cut(s) 488, 836, 2269
NdeII GATC 8 cut(s) 339, 514, 994, 1100, 1553, 1738, 1948, 2250
NlaIII CATG 9 cut(s) 639, 759, 910, 1277, 1538, 2086, 2120, 2124, 2248
NlaIV GGNNCC 6 cut(s) 369, 720, 845, 912, 934, 1069
NmuCI GTSAC 1 cut(s) 1826
NsiI ATGCAT 3 cut(s) 24, 1016, 2122
NspI RCATGY 2 cut(s) 2120, 2248
PaeI GCATGC 2 cut(s) 2120, 2248
PceI AGGCCT 2 cut(s) 1080, 1897
PciSI GCTCTTC 1 cut(s) 347
PctI GAATGC 1 cut(s) 1125
PfeI GAWTC 4 cut(s) 865, 1284, 1729, 1886
PfoI TCCNGGA 1 cut(s) 876
PinAI ACCGGT 1 cut(s) 186
PkrI GCNGC 5 cut(s) 573, 641, 769, 1333, 1591
Ple19I CGATCG 1 cut(s) 342
PleI GAGTC 4 cut(s) 577, 760, 1572, 1815
PpsI GAGTC 4 cut(s) 577, 760, 1572, 1815
Ppu21I YACGTR 1 cut(s) 304
PshAI GACNNNNGTC 1 cut(s) 1026
Psp124BI GAGCTC 1 cut(s) 288
Psp6I CCWGG 6 cut(s) 356, 714, 850, 949, 1914, 2170
PspEI GGTNACC 1 cut(s) 446
PspFI CCCAGC 1 cut(s) 1746
PspGI CCWGG 6 cut(s) 356, 714, 850, 949, 1914, 2170
PspN4I GGNNCC 6 cut(s) 369, 720, 845, 912, 934, 1069
PspPI GGNCC 1 cut(s) 2168
PstI CTGCAG 1 cut(s) 926
PstNI CAGNNNCTG 2 cut(s) 357, 586
PsuI RGATCY 2 cut(s) 1100, 2250
PvuI CGATCG 1 cut(s) 342
PvuII CAGCTG 1 cut(s) 1592
RsaI GTAC 7 cut(s) 302, 350, 467, 481, 983, 2218, 2224
RsaNI GTAC 7 cut(s) 301, 349, 466, 480, 982, 2217, 2223
RseI CAYNNNNRTG 6 cut(s) 418, 615, 790, 1440, 1533, 1653
SacI GAGCTC 1 cut(s) 288
SaqAI TTAA 6 cut(s) 678, 1161, 1937, 1983, 2045, 2261
SatI GCNGC 5 cut(s) 572, 640, 768, 1332, 1590
Sau3AI GATC 8 cut(s) 339, 514, 994, 1100, 1553, 1738, 1948, 2250
Sau96I GGNCC 1 cut(s) 2168
ScaI AGTACT 1 cut(s) 2218
SchI GAGTC 4 cut(s) 577, 761, 1572, 1816
ScrFI CCNGG 8 cut(s) 358, 452, 716, 852, 878, 951, 1916, 2172
SduI GDGCHC 4 cut(s) 288, 785, 1530, 1609
SfaNI GCATC 9 cut(s) 9, 40, 313, 410, 782, 1001, 1023, 1626, 2173
SfcI CTRYAG 3 cut(s) 296, 922, 1834
SmiMI CAYNNNNRTG 6 cut(s) 418, 615, 790, 1440, 1533, 1653
SmlI CTYRAG 3 cut(s) 327, 1250, 1580
SmoI CTYRAG 3 cut(s) 327, 1250, 1580
SpeI ACTAGT 1 cut(s) 1489
SphI GCATGC 2 cut(s) 2120, 2248
SseBI AGGCCT 2 cut(s) 1080, 1897
SsiI CCGC 1 cut(s) 221
SspI AATATT 1 cut(s) 1326
SspMI CTAG 6 cut(s) 71, 1064, 1200, 1490, 1610, 2214
SstI GAGCTC 1 cut(s) 288
StuI AGGCCT 2 cut(s) 1080, 1897
StyD4I CCNGG 8 cut(s) 356, 450, 714, 850, 876, 949, 1914, 2170
StyI CCWWGG 2 cut(s) 1288, 2082
TaaI ACNGT 8 cut(s) 125, 281, 300, 353, 367, 971, 1677, 2014
TaiI ACGT 4 cut(s) 306, 407, 485, 1833
TaqI TCGA 9 cut(s) 180, 252, 342, 513, 694, 1030, 1651, 1968, 1998
TaqII GACCGA 1 cut(s) 810
TatI WGTACW 2 cut(s) 465, 2216
TfiI GAWTC 4 cut(s) 865, 1284, 1729, 1886
Tru1I TTAA 6 cut(s) 678, 1161, 1937, 1983, 2045, 2261
Tru9I TTAA 6 cut(s) 678, 1161, 1937, 1983, 2045, 2261
TscAI CASTG 5 cut(s) 111, 316, 571, 1078, 2019
TseFI GTSAC 1 cut(s) 1826
TseI GCWGC 5 cut(s) 571, 639, 767, 1331, 1589
Tsp45I GTSAC 1 cut(s) 1826
TspDTI ATGAA 5 cut(s) 1290, 1554, 1698, 1974, 2084
TspRI CASTG 5 cut(s) 111, 316, 571, 1078, 2019
XapI RAATTY 4 cut(s) 626, 1230, 1962, 2136
XceI RCATGY 2 cut(s) 2120, 2248
XspI CTAG 6 cut(s) 71, 1064, 1200, 1490, 1610, 2214
ZraI GACGTC 1 cut(s) 1831
ZrmI AGTACT 1 cut(s) 2218
Zsp2I ATGCAT 3 cut(s) 24, 1016, 2122
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.