Rroxscaffold_1G00060320

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000066
Physical Location & Seq
Forward (+)
82578098 .. 82583320
5223 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00060320.1

Sequence Viewer

Length: 2418 bp
ATGCTTATTATGCAACTGATCTCTTTAGCAATAGTGGGAGTACTGGTTGTAACAATAACAACTGAAACAATCACATCAGCAGAAGCAGCAGCTCAGTCCTTGCCAGGATGCAAAACCCAGTGTGGTAATCTGATGATTCCTTACCCATTTGGCATAGGTGACGGTTGTTACTTGCGACCAGAATTCAACATCACTTGTGACCATTCCACCACACCTCCATCAGCAAACTTCACGAATTATAGCATACGTATTGCTCACATATCCCTTGCTGAGGGTGAGTTGCGAATAATGCAAGACGTAGGTGTAGAATGTTATGACACCCAGGGTCATGAAACGGACTATAACTGGCCTTCTCTCCAGTTGCCTCCTCCTTATACCATCTCTGATACCAAAAACAAGTTCTTTGACATTGGCTGTGGCACTGTAGCTATTTTTCAAGGTGACCGCACGCACCCTGGGCCAGATGAAGATAAGTCCACAGCGGGGTACACCATGGTCTTATGTGATGATCTGCTTGGCAAAGTACTTACAAACTCTTGCAATGGCGTTGGCTGTTCCCAGGTTCCTATCCCAAGTGGATTGCACAACTTTACAATAATTCTGAGTCCAATTGCGGATAATACAGGTACATGGTTGACTAGGTATCCTTGTAGCTATTCCTTCATTGTGGAAGTAGCCATGTTCACATTCTCCCCCGATACAAGTTTTGATTTACTGAATACCACCAGCCAGCTTCCACTCATTGTTAATTGGGGGATTGGGGATGAACCATGTGATGAAAAGAGCCAGAATTATGCATGCAAGGCAGAAAATAGCAAGTGTGTCAACCAGTCCATCATTAATGGGCCGTCTGGTTACATTTGCCAATGCTTACCAGGCTATGAAGGGAATCCATACCTCGAAGATGGGTGCCAAGATATTGATGAGTGCAAGTCTTCAAACCCCTGCAACATTGGAATGTGCATCAATTCACCTCCAGGAAATTACTCTTGTTTATGTCCCAAAGGATACAAAAATGATGGCACAAATCGACAGAGTTGCATCAAAGACGATACTAAAAACAACTCAAAGATAACTGTCCTGCTTACTATCGCATTAGGTGTAAGTGTAGGCTTCTTGGTTTTTTTCATTGGAATTTCATGGACATCTTGGGGAATAAAGAAAAGAAACTTCATTAAGCTCAAAGAGAGGTACTTTAAAGAAAATGGTGGTTTGTTATTGCAAAAACAACTCTTTCATCATGGAGGCTCTGTGGAGACAACAAGAATTTTTACTGCAGAAGAACTTGAGAAGGCCACAAACAATTACCATGAAAGTAGAGTCCTTGGTGAAGGAGGATATGGAACCGTTTATAAAGGCATACTACTAGATAACAAAGTGGTTGCCATTAAAAAGTCTAAAATTGCTGCCCCGGCCCAGAGTGATCAATTTGTTAATGAGGTGATTGTTCTTTCTCAAATCAATCACAGAAATGTAGTCAGGCTATTAGGTTGTTGCTTAGAGACAGAAACACCTTTACTGATTTATGAGTTCATCACCAATGGCACTCTTTATGAGCACATTCATAAGAAAAGATCACTACTTTCTTTGGAATTACGAATGAAGATAGCAGCTGAAACTGCAGGAGCACTAGCATACCTACATTCCTCAACTTCCATGCCAATTATACATCGAGATGTGAAAGCAATGAATATACTATTAGATGATAACTATACAGCAAAAGTGGCAGACTTTGGAGCGTCACGATTAATTCCTTTAGGTCAAACTGAATTAGAAACTTTAGTACTAGGGACATTTGGTTACCTCGACCCTGAATATTTGCAATCAAACCAACTAACAGAAAAGAGTGACGTTTACAGCTTTGGAGTTGTCCTGCTGGAGCTAATAACTAGCAAAGTGGCACTTTGTACAAATAGATGCTTAGCAAGCATCTTCATCTCTGCCATGGAAGAAGGTTGGCTGGATCAAATTCTTGATGATACTATAGTGAATGATGGAAATATGGAGATGGTAAAAATTGTAGCCAATCTTGCAAAAAGATGTTTAAGTGTAAAAGGGCAGGAGAGGCCAACCATGAAAGAGGTGGCGATGGAGCTAGAAGGAATGAGAATGATGGCAAAGCATCCATGGAGAAACAATGCTGATTTCTGTCCAGAAGAGAACGAGAGTTTGCTCAGTTCACTTGATGCAAACACTCACGTGGTGGATATTAGAGGTGATGGTGGTTCTGGCAGTACAAGTGTACAACTACCAGGACCTGCAAAGAATGTTGATTTGGTCTTCCAGCAGGCCGTTCATGTTAAGAAACGATCAATGCTGTTGATGAGTAGAATCCAGGCTGAATGTTATTGTCTTTTTGTTAGGCCGGCTTCTCCAATCAAATCGGCAGGCTCTCATTTTTTCATTCTCTTTAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK hEGF

Protein Analysis

805

Amino Acids

88.63

Weight (kDa)

5.44

Isoelectric Point (pI)

33.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 37 - 97 3.5e-11 Wall-associated receptor kinase galacturonan-binding
Pkinase PF00069 436 - 695 4.7e-48 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 439 - 699 5.9e-48 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21210 AT1G21230 AT1G21240 AT1G21240 AT1G21245 AT1G21250 AT1G21270 AT1G22720 AT3G25490
fragaria_vesca FvH4_2g35051 FvH4_2g35051 FvH4_2g35060 FvH4_3g10281 FvH4_3g10285 FvH4_3g10286 FvH4_3g10311 FvH4_3g10311 FvH4_3g10320 FvH4_3g10332 FvH4_3g10340 FvH4_3g10350 FvH4_3g10360 FvH4_3g10390 FvH4_3g10412 FvH4_3g10413 FvH4_3g10461 FvH4_3g10462 FvH4_3g10470 FvH4_3g10510 FvH4_3g10520 FvH4_3g10530 FvH4_3g10550 FvH4_3g10560 FvH4_3g10611 FvH4_3g11730 FvH4_6g53140 FvH4_6g53142 FvH4_7g31880 FvH4_7g31910 FvH4_7g31920
malus_domestica MD05G1269900.v1.1 MD05G1270000.v1.1 MD05G1270200.v1.1 MD05G1270400.v1.1 MD05G1270500.v1.1 MD05G1270600.v1.1 MD05G1270800.v1.1 MD05G1270900.v1.1 MD07G1183800.v1.1 MD08G1107600.v1.1 MD10G1248500.v1.1 MD10G1249200.v1.1 MD10G1249500.v1.1 MD10G1250000.v1.1 MD10G1250500.v1.1 MD10G1250900.v1.1 MD10G1251200.v1.1 MD10G1251400.v1.1 MD13G1004600.v1.1 MD15G1088400.v1.1
prunus_persica Prupe.1G442400_v2.0.a1 Prupe.1G442500_v2.0.a1 Prupe.2G223400_v2.0.a1 Prupe.4G091900_v2.0.a1 Prupe.4G093200_v2.0.a1 Prupe.4G093300_v2.0.a1 Prupe.4G093400_v2.0.a1 Prupe.4G093500_v2.0.a1 Prupe.4G094100_v2.0.a1 Prupe.4G094200_v2.0.a1 Prupe.4G094300_v2.0.a1 Prupe.6G358300_v2.0.a1
pyrus_communis pycom08g08990 pycom10g20660 pycom10g20670 pycom10g20680 pycom10g20710 pycom10g20740 pycom13g00400 pycom15g08290
rosa_chinensis RchiOBHm_Chr1g0326861 RchiOBHm_Chr1g0326871 RchiOBHm_Chr1g0331391 RchiOBHm_Chr1g0336911 RchiOBHm_Chr1g0364001 RchiOBHm_Chr2g0174541 RchiOBHm_Chr2g0175041 RchiOBHm_Chr2g0175101 RchiOBHm_Chr2g0175121 RchiOBHm_Chr3g0486331 RchiOBHm_Chr5g0015581 RchiOBHm_Chr5g0016551 RchiOBHm_Chr5g0016561 RchiOBHm_Chr5g0016581 RchiOBHm_Chr5g0016601 RchiOBHm_Chr5g0016611 RchiOBHm_Chr5g0016641 RchiOBHm_Chr5g0016661 RchiOBHm_Chr5g0016691 RchiOBHm_Chr5g0016701 RchiOBHm_Chr5g0016731 RchiOBHm_Chr5g0016741 RchiOBHm_Chr5g0016811 RchiOBHm_Chr5g0016841 RchiOBHm_Chr5g0016871 RchiOBHm_Chr5g0016881 RchiOBHm_Chr5g0016931 RchiOBHm_Chr5g0017021 RchiOBHm_Chr5g0017041 RchiOBHm_Chr5g0017071 RchiOBHm_Chr5g0017091 RchiOBHm_Chr5g0017121 RchiOBHm_Chr5g0017141 RchiOBHm_Chr5g0017291 RchiOBHm_Chr5g0017321 RchiOBHm_Chr5g0017401 RchiOBHm_Chr5g0019001 RchiOBHm_Chr5g0019071 RchiOBHm_Chr5g0028941 RchiOBHm_Chr5g0052141 RchiOBHm_Chr5g0054721 RchiOBHm_Chr5g0054801 RchiOBHm_Chr5g0054931 RchiOBHm_Chr5g0054941 RchiOBHm_Chr6g0259291 RchiOBHm_Chr6g0292991 RchiOBHm_Chr6g0306911
rosa_laevigata RLG00000009465 RLG00000009468 RLG00000010755 RLG00000011976 RLG00000014541 RLG00000014542 RLG00000015169 RLG00000022308 RLG00000027529 RLG00000029324 RLG00000030244 RLG00000030245 RLG00000032210 RLG00000032211 RLG00000032257 RLG00000032259 RLG00000032260 RLG00000032261 RLG00000032262 RLG00000032268 RLG00000032269 RLG00000032272 RLG00000032282 RLG00000032283 RLG00000032308 RLG00000032413 RLG00000033129 RLG00000034945
rosa_multiflora Rmu_co8241929.1_g000001 Rmu_co8269227.1_g000001 Rmu_co8461259.1_g000001 Rmu_co8495385.1_g000001 Rmu_sc0000028.1_g000003 Rmu_sc0000083.1_g000009 Rmu_sc0000083.1_g000010 Rmu_sc0000083.1_g000031 Rmu_sc0000083.1_g000043 Rmu_sc0000083.1_g000046 Rmu_sc0000083.1_g000047 Rmu_sc0000563.1_g000024 Rmu_sc0000842.1_g000012 Rmu_sc0001235.1_g000001 Rmu_sc0001235.1_g000005 Rmu_sc0001235.1_g000009 Rmu_sc0001235.1_g000015 Rmu_sc0001235.1_g000016 Rmu_sc0001235.1_g000017 Rmu_sc0001473.1_g000036 Rmu_sc0001607.1_g000016 Rmu_sc0001788.1_g000006 Rmu_sc0002018.1_g000008 Rmu_sc0002028.1_g000001 Rmu_sc0002680.1_g000011 Rmu_sc0002798.1_g000023 Rmu_sc0002886.1_g000023 Rmu_sc0002989.1_g000012 Rmu_sc0003064.1_g000009 Rmu_sc0003382.1_g000006 Rmu_sc0003630.1_g000002 Rmu_sc0003630.1_g000047 Rmu_sc0003630.1_g000066 Rmu_sc0003630.1_g000067 Rmu_sc0003649.1_g000010 Rmu_sc0003688.1_g000005 Rmu_sc0003920.1_g000007 Rmu_sc0004765.1_g000007 Rmu_sc0004765.1_g000008 Rmu_sc0004920.1_g000011 Rmu_sc0005223.1_g000008 Rmu_sc0005223.1_g000010 Rmu_sc0005223.1_g000015 Rmu_sc0005223.1_g000016 Rmu_sc0005319.1_g000014 Rmu_sc0005319.1_g000015 Rmu_sc0005319.1_g000018 Rmu_sc0005319.1_g000019 Rmu_sc0005319.1_g000025 Rmu_sc0005418.1_g000007 Rmu_sc0005418.1_g000012 Rmu_sc0006106.1_g000011 Rmu_sc0006914.1_g000012 Rmu_sc0007023.1_g000001 Rmu_sc0007662.1_g000001 Rmu_sc0007920.1_g000002 Rmu_sc0007920.1_g000013 Rmu_sc0008053.1_g000016 Rmu_sc0008053.1_g000017 Rmu_sc0008053.1_g000018 Rmu_sc0008053.1_g000023 Rmu_sc0008053.1_g000050 Rmu_sc0009042.1_g000007 Rmu_sc0009042.1_g000008 Rmu_sc0010642.1_g000008 Rmu_sc0011109.1_g000002 Rmu_sc0011314.1_g000006 Rmu_sc0011974.1_g000006 Rmu_sc0012179.1_g000009 Rmu_sc0015169.1_g000001 Rmu_sc0016368.1_g000002 Rmu_sc0022659.1_g000001 Rmu_sc0027371.1_g000001 Rmu_sc0028213.1_g000001 Rmu_sc0034526.1_g000001 Rmu_sc0039366.1_g000002 Rmu_ssc0000050.1_g000084 Rmu_ssc0000050.1_g000088 Rmu_ssc0000167.1_g000017
rosa_roxburghii Rroxscaffold_1G00011340 Rroxscaffold_1G00025710 Rroxscaffold_1G00025870 Rroxscaffold_1G00026070 Rroxscaffold_1G00026170 Rroxscaffold_1G00026200 Rroxscaffold_1G00026270 Rroxscaffold_1G00050950 Rroxscaffold_1G00058900 Rroxscaffold_1G00060040 Rroxscaffold_1G00060060 Rroxscaffold_1G00060070 Rroxscaffold_1G00060080 Rroxscaffold_1G00060110 Rroxscaffold_1G00060120 Rroxscaffold_1G00060140 Rroxscaffold_1G00060170 Rroxscaffold_1G00060210 Rroxscaffold_1G00060220 Rroxscaffold_1G00060240 Rroxscaffold_1G00060250 Rroxscaffold_1G00060260 Rroxscaffold_1G00060300 Rroxscaffold_1G00060320 Rroxscaffold_1G00060330 Rroxscaffold_1G00060900 Rroxscaffold_1G00060920 Rroxscaffold_1G00073980 Rroxscaffold_2G00077560 Rroxscaffold_2G00077580 Rroxscaffold_2G00146990 Rroxscaffold_3G00236200 Rroxscaffold_3G00236210 Rroxscaffold_3G00236220 Rroxscaffold_4G00292760 Rroxscaffold_4G00315200 Rroxscaffold_4G00315240 Rroxscaffold_4G00326240 Rroxscaffold_4G00328980 Rroxscaffold_5G00341890 Rroxscaffold_7G00161350 Rroxscaffold_7G00174490 Rroxscaffold_7G00206550
rosa_rugosa Rorug01G0093000 Rorug01G0093100 Rorug01G0128800 Rorug01G0312000 Rorug01G0312000 Rorug02G0581100 Rorug03G0224600 Rorug05G0028500 Rorug05G0032700 Rorug05G0032800 Rorug05G0032900 Rorug05G0032900 Rorug05G0033000 Rorug05G0033100 Rorug05G0033300 Rorug05G0033400 Rorug05G0033900 Rorug05G0034900 Rorug05G0035000 Rorug05G0046900 Rorug05G0047500 Rorug05G0047500 Rorug05G0111600 Rorug05G0287900 Rorug05G0288700 Rorug05G0586900 Rorug06G0231700 Rorug06G0354300 Rorug06G0354400
rosa_samantha Rh1DG122200 Rh2DG686800 Rh5BG136600 Rh6AG344500 Rh6BG351900 Rh6CG359000 Rh6CG359100 Rh6CG387600 Rh6CG480500 Rh6DG344600 Rh6DG467400
rosa_wichuraiana Rw0G010250 Rw0G014820 Rw0G021400 Rw0G023100 Rw1G009450 Rw1G012100 Rw1G012180 Rw1G028360 Rw2G054260 Rw3G024370 Rw3G024420 Rw3G024470 Rw5G010850 Rw5G011010 Rw5G011020 Rw5G011050 Rw5G011080 Rw5G011120 Rw5G012300 Rw5G018470 Rw5G033830 Rw6G008930 Rw6G030070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1353
Acc36I ACCTGC 1 cut(s) 2266
AccB1I GGYRCC 1 cut(s) 909
AciI CCGC 3 cut(s) 445, 482, 614
AclWI GGATC 1 cut(s) 1971
AcsI RAATTY 4 cut(s) 182, 1134, 1266, 1968
AcvI CACGTG 1 cut(s) 2200
AdeI CACNNNGTG 1 cut(s) 2203
AfaI GTAC 9 cut(s) 42, 488, 525, 628, 1193, 1785, 1909, 2236, 2244
AfiI CCNNNNNNNGG 2 cut(s) 271, 483
AgsI TTSAA 3 cut(s) 187, 437, 939
AjnI CCWGG 8 cut(s) 103, 321, 454, 558, 874, 976, 2251, 2334
AjuI GAANNNNNNNTTGG 4 cut(s) 383, 415, 2258, 2290
AleI CACNNNNGTG 1 cut(s) 2198
AluBI AGCT 9 cut(s) 92, 428, 654, 733, 1180, 1613, 1860, 1882, 2095
AluI AGCT 9 cut(s) 92, 428, 654, 733, 1180, 1613, 1860, 1882, 2095
Alw21I GWGCWC 2 cut(s) 1560, 1630
Alw26I GTCTC 2 cut(s) 1250, 1496
AlwI GGATC 1 cut(s) 1971
AlwNI CAGNNNCTG 1 cut(s) 2258
AoxI GGCC 8 cut(s) 347, 458, 845, 1293, 1413, 2066, 2289, 2363
ApeKI GCWGC 4 cut(s) 86, 89, 1406, 1610
ApoI RAATTY 4 cut(s) 182, 1134, 1266, 1968
AseI ATTAAT 2 cut(s) 840, 1748
AspS9I GGNCC 4 cut(s) 458, 845, 1414, 2255
AsuC2I CCSGG 1 cut(s) 1412
AsuHPI GGTGA 8 cut(s) 170, 287, 452, 963, 1340, 1453, 1528, 2228
AvaII GGWCC 1 cut(s) 2255
BaeI ACNNNNGTAYC 2 cut(s) 1971, 2004
BanI GGYRCC 1 cut(s) 909
BbrPI CACGTG 1 cut(s) 2200
BbsI GAAGAC 2 cut(s) 927, 2272
Bbv12I GWGCWC 2 cut(s) 1560, 1630
BbvCI CCTCAGC 1 cut(s) 270
BbvI GCAGC 4 cut(s) 98, 101, 1393, 1622
BceAI ACGGC 2 cut(s) 832, 2276
BcgI CGANNNNNNTGC 2 cut(s) 1020, 1054
BciT130I CCWGG 8 cut(s) 105, 323, 456, 560, 876, 978, 2253, 2336
BciVI GTATCC 2 cut(s) 654, 1001
BclI TGATCA 1 cut(s) 1423
BcnI CCSGG 1 cut(s) 1412
BcoDI GTCTC 2 cut(s) 1250, 1496
BfaI CTAG 6 cut(s) 639, 1367, 1631, 1787, 1890, 2096
BfmI CTRYAG 4 cut(s) 423, 1275, 1620, 1983
BfuAI ACCTGC 1 cut(s) 2266
BfuI GTATCC 2 cut(s) 654, 1001
BisI GCNGC 4 cut(s) 87, 90, 1407, 1611
BlpI GCTNAGC 1 cut(s) 1921
BlsI GCNGC 4 cut(s) 88, 91, 1408, 1612
BmcAI AGTACT 3 cut(s) 42, 525, 1785
Bme1390I CCNGG 9 cut(s) 105, 323, 456, 560, 876, 978, 1412, 2253, 2336
Bme18I GGWCC 1 cut(s) 2255
BmgT120I GGNCC 4 cut(s) 458, 845, 1414, 2255
BmiI GGNNCC 3 cut(s) 564, 911, 1345
BmrFI CCNGG 9 cut(s) 105, 323, 456, 560, 876, 978, 1412, 2253, 2336
BmrI ACTGGG 1 cut(s) 112
BmsI GCATC 7 cut(s) 98, 972, 1050, 1907, 1938, 2131, 2176
BmuI ACTGGG 1 cut(s) 112
BpiI GAAGAC 2 cut(s) 927, 2272
BpmI CTGGAG 3 cut(s) 341, 960, 1898
Bpu10I CCTNAGC 1 cut(s) 270
Bpu1102I GCTNAGC 1 cut(s) 1921
BpuEI CTTGAG 1 cut(s) 1307
BpuMI CCSGG 1 cut(s) 1412
BsaAI YACGTR 2 cut(s) 248, 2200
BsaXI ACNNNNNCTCC 6 cut(s) 30, 60, 199, 229, 1236, 1266
Bsc4I CCNNNNNNNGG 2 cut(s) 271, 483
Bse118I RCCGGY 1 cut(s) 2365
Bse1I ACTGG 5 cut(s) 48, 118, 350, 358, 829
Bse3DI GCAATG 2 cut(s) 547, 1692
BseBI CCWGG 8 cut(s) 105, 323, 456, 560, 876, 978, 2253, 2336
BseGI GGATG 3 cut(s) 113, 769, 2122
BseLI CCNNNNNNNGG 2 cut(s) 271, 483
BseMI GCAATG 2 cut(s) 547, 1692
BseMII CTCAG 4 cut(s) 107, 261, 593, 2188
BseNI ACTGG 5 cut(s) 48, 118, 350, 358, 829
BseRI GAGGAG 1 cut(s) 357
BseXI GCAGC 4 cut(s) 98, 101, 1393, 1622
BshFI GGCC 8 cut(s) 349, 460, 847, 1295, 1415, 2068, 2291, 2365
BshNI GGYRCC 1 cut(s) 909
BsiHKAI GWGCWC 2 cut(s) 1560, 1630
BsiSI CCGG 2 cut(s) 1412, 2366
BslFI GGGAC 2 cut(s) 984, 1804
BslI CCNNNNNNNGG 2 cut(s) 271, 483
BsmAI GTCTC 2 cut(s) 1250, 1496
BsmFI GGGAC 2 cut(s) 984, 1804
BsnI GGCC 8 cut(s) 349, 460, 847, 1295, 1415, 2068, 2291, 2365
Bsp1286I GDGCHC 2 cut(s) 1560, 1630
Bsp1407I TGTACA 2 cut(s) 1907, 2242
Bsp143I GATC 6 cut(s) 18, 508, 1423, 1574, 1963, 2309
Bsp1720I GCTNAGC 1 cut(s) 1921
Bsp19I CCATGG 3 cut(s) 492, 1944, 2126
BspACI CCGC 3 cut(s) 445, 482, 614
BspANI GGCC 8 cut(s) 349, 460, 847, 1295, 1415, 2068, 2291, 2365
BspCNI CTCAG 4 cut(s) 106, 262, 594, 2187
BspHI TCATGA 1 cut(s) 328
BspLI GGNNCC 3 cut(s) 564, 911, 1345
BspMAI CTGCAG 2 cut(s) 1279, 1624
BspMI ACCTGC 1 cut(s) 2266
BspPI GGATC 1 cut(s) 1971
BspT107I GGYRCC 1 cut(s) 909
BsrDI GCAATG 2 cut(s) 547, 1692
BsrFI RCCGGY 1 cut(s) 2365
BsrGI TGTACA 2 cut(s) 1907, 2242
BsrI ACTGG 5 cut(s) 48, 118, 350, 358, 829
BssAI RCCGGY 1 cut(s) 2365
BssMI GATC 6 cut(s) 18, 508, 1423, 1574, 1963, 2309
BssT1I CCWWGG 4 cut(s) 492, 1324, 1944, 2126
Bst2UI CCWGG 8 cut(s) 105, 323, 456, 560, 876, 978, 2253, 2336
Bst4CI ACNGT 4 cut(s) 164, 424, 1078, 1348
Bst6I CTCTTC 1 cut(s) 2151
BstAUI TGTACA 2 cut(s) 1907, 2242
BstBAI YACGTR 2 cut(s) 248, 2200
BstC8I GCNNGC 7 cut(s) 449, 731, 799, 1927, 2289, 2367, 2389
BstDEI CTNAG 6 cut(s) 93, 270, 602, 1498, 1921, 2174
BstDSI CCRYGG 3 cut(s) 492, 1944, 2126
BstEII GGTNACC 2 cut(s) 440, 1799
BstENI CCTNNNNNAGG 1 cut(s) 269
BstF5I GGATG 3 cut(s) 113, 769, 2122
BstKTI GATC 6 cut(s) 21, 511, 1426, 1577, 1966, 2312
BstMAI GTCTC 2 cut(s) 1250, 1496
BstMBI GATC 6 cut(s) 18, 508, 1423, 1574, 1963, 2309
BstNI CCWGG 8 cut(s) 105, 323, 456, 560, 876, 978, 2253, 2336
BstNSI RCATGY 1 cut(s) 801
BstPI GGTNACC 2 cut(s) 440, 1799
BstSCI CCNGG 9 cut(s) 103, 321, 454, 558, 874, 976, 1410, 2251, 2334
BstSFI CTRYAG 4 cut(s) 423, 1275, 1620, 1983
BstSNI TACGTA 1 cut(s) 248
BstV1I GCAGC 4 cut(s) 98, 101, 1393, 1622
BstV2I GAAGAC 2 cut(s) 927, 2272
BsuI GTATCC 2 cut(s) 654, 1001
BsuRI GGCC 8 cut(s) 349, 460, 847, 1295, 1415, 2068, 2291, 2365
BtgI CCRYGG 3 cut(s) 492, 1944, 2126
BtgZI GCGATG 1 cut(s) 2102
BtsCI GGATG 3 cut(s) 113, 769, 2122
BtsIMutI CAGTG 2 cut(s) 125, 420
BveI ACCTGC 1 cut(s) 2266
Cac8I GCNNGC 7 cut(s) 449, 731, 799, 1927, 2289, 2367, 2389
CaiI CAGNNNCTG 1 cut(s) 2258
CciI TCATGA 1 cut(s) 328
Cfr10I RCCGGY 1 cut(s) 2365
Cfr13I GGNCC 4 cut(s) 458, 845, 1414, 2255
CseI GACGC 1 cut(s) 1728
Csp6I GTAC 9 cut(s) 41, 487, 524, 627, 1192, 1784, 1908, 2235, 2243
CviQI GTAC 9 cut(s) 41, 487, 524, 627, 1192, 1784, 1908, 2235, 2243
DdeI CTNAG 6 cut(s) 93, 270, 602, 1498, 1921, 2174
DpnI GATC 6 cut(s) 20, 510, 1425, 1576, 1965, 2311
DpnII GATC 6 cut(s) 18, 508, 1423, 1574, 1963, 2309
DraI TTTAAA 1 cut(s) 1198
DraIII CACNNNGTG 1 cut(s) 2203
Eam1104I CTCTTC 1 cut(s) 2151
EarI CTCTTC 1 cut(s) 2151
Eco105I TACGTA 1 cut(s) 248
Eco130I CCWWGG 4 cut(s) 492, 1324, 1944, 2126
Eco47I GGWCC 1 cut(s) 2255
Eco72I CACGTG 1 cut(s) 2200
Eco91I GGTNACC 2 cut(s) 440, 1799
EcoNI CCTNNNNNAGG 1 cut(s) 269
EcoO109I RGGNCCY 1 cut(s) 2255
EcoO65I GGTNACC 2 cut(s) 440, 1799
EcoRI GAATTC 1 cut(s) 182
EcoRII CCWGG 8 cut(s) 103, 321, 454, 558, 874, 976, 2251, 2334
EcoT14I CCWWGG 4 cut(s) 492, 1324, 1944, 2126
EcoT22I ATGCAT 1 cut(s) 799
ErhI CCWWGG 4 cut(s) 492, 1324, 1944, 2126
FalI AAGNNNNNCTT 2 cut(s) 1887, 1919
FaqI GGGAC 2 cut(s) 984, 1804
FauI CCCGC 1 cut(s) 475
FbaI TGATCA 1 cut(s) 1423
Fnu4HI GCNGC 4 cut(s) 87, 90, 1407, 1611
FokI GGATG 3 cut(s) 120, 776, 2109
Fsp4HI GCNGC 4 cut(s) 87, 90, 1407, 1611
FspBI CTAG 6 cut(s) 639, 1367, 1631, 1787, 1890, 2096
GluI GCNGC 4 cut(s) 87, 90, 1407, 1611
GsuI CTGGAG 3 cut(s) 341, 960, 1898
HaeIII GGCC 8 cut(s) 349, 460, 847, 1295, 1415, 2068, 2291, 2365
HapII CCGG 2 cut(s) 1412, 2366
HgaI GACGC 1 cut(s) 1728
HincII GTYRAC 2 cut(s) 636, 826
HindII GTYRAC 2 cut(s) 636, 826
HinfI GANTC 5 cut(s) 136, 604, 889, 1320, 2331
HpaII CCGG 2 cut(s) 1412, 2366
HphI GGTGA 8 cut(s) 170, 287, 452, 963, 1340, 1453, 1528, 2228
Hpy166II GTNNAC 8 cut(s) 477, 489, 636, 684, 826, 1855, 2180, 2243
Hpy188I TCNGA 3 cut(s) 132, 385, 603
Hpy188III TCNNGA 6 cut(s) 232, 329, 1673, 1743, 1973, 2153
Hpy8I GTNNAC 8 cut(s) 477, 489, 636, 684, 826, 1855, 2180, 2243
HpyAV CCTTC 7 cut(s) 360, 670, 878, 1285, 1325, 1946, 2093
HpyCH4III ACNGT 4 cut(s) 164, 424, 1078, 1348
HpyCH4IV ACGT 4 cut(s) 247, 297, 1851, 2199
HpyF3I CTNAG 6 cut(s) 93, 270, 602, 1498, 1921, 2174
HpySE526I ACGT 4 cut(s) 247, 297, 1851, 2199
KroI GCCGGC 1 cut(s) 2365
KroNI GCCGGC 1 cut(s) 2367
Ksp22I TGATCA 1 cut(s) 1423
Kzo9I GATC 6 cut(s) 18, 508, 1423, 1574, 1963, 2309
LmnI GCTCC 4 cut(s) 1625, 1736, 1879, 2092
Lsp1109I GCAGC 4 cut(s) 98, 101, 1393, 1622
LweI GCATC 7 cut(s) 98, 972, 1050, 1907, 1938, 2131, 2176
MaeI CTAG 6 cut(s) 639, 1367, 1631, 1787, 1890, 2096
MaeII ACGT 4 cut(s) 247, 297, 1851, 2199
MaeIII GTNAC 9 cut(s) 49, 158, 167, 197, 440, 854, 1740, 1799, 1847
MalI GATC 6 cut(s) 20, 510, 1425, 1576, 1965, 2311
MboI GATC 6 cut(s) 18, 508, 1423, 1574, 1963, 2309
MboII GAAGA 9 cut(s) 479, 914, 927, 1292, 1615, 1924, 1961, 2168, 2272
MfeI CAATTG 1 cut(s) 609
MhlI GDGCHC 2 cut(s) 1560, 1630
MlyI GAGTC 2 cut(s) 613, 1329
Mph1103I ATGCAT 1 cut(s) 799
MroNI GCCGGC 1 cut(s) 2365
MslI CAYNNNNRTG 4 cut(s) 956, 1470, 1674, 2198
MspA1I CMGCKG 2 cut(s) 482, 1613
MspI CCGG 2 cut(s) 1412, 2366
MspR9I CCNGG 9 cut(s) 105, 323, 456, 560, 876, 978, 1412, 2253, 2336
MunI CAATTG 1 cut(s) 609
MvaI CCWGG 8 cut(s) 105, 323, 456, 560, 876, 978, 2253, 2336
NaeI GCCGGC 1 cut(s) 2367
NciI CCSGG 1 cut(s) 1412
NcoI CCATGG 3 cut(s) 492, 1944, 2126
NdeII GATC 6 cut(s) 18, 508, 1423, 1574, 1963, 2309
NgoMIV GCCGGC 1 cut(s) 2365
NlaIV GGNNCC 3 cut(s) 564, 911, 1345
NmuCI GTSAC 5 cut(s) 158, 197, 440, 1740, 1847
NsiI ATGCAT 1 cut(s) 799
NspI RCATGY 1 cut(s) 801
OliI CACNNNNGTG 1 cut(s) 2198
PaeI GCATGC 1 cut(s) 801
PagI TCATGA 1 cut(s) 328
PasI CCCWGGG 2 cut(s) 322, 455
PdiI GCCGGC 1 cut(s) 2367
PfeI GAWTC 3 cut(s) 136, 889, 2331
PfoI TCCNGGA 1 cut(s) 976
PkrI GCNGC 4 cut(s) 88, 91, 1408, 1612
PleI GAGTC 2 cut(s) 612, 1328
PmaCI CACGTG 1 cut(s) 2200
PmlI CACGTG 1 cut(s) 2200
PpsI GAGTC 2 cut(s) 612, 1328
Ppu21I YACGTR 2 cut(s) 248, 2200
PpuMI RGGWCCY 1 cut(s) 2255
PshBI ATTAAT 2 cut(s) 840, 1748
PsiI TTATAA 1 cut(s) 1353
Psp5II RGGWCCY 1 cut(s) 2255
Psp6I CCWGG 8 cut(s) 103, 321, 454, 558, 874, 976, 2251, 2334
PspCI CACGTG 1 cut(s) 2200
PspEI GGTNACC 2 cut(s) 440, 1799
PspGI CCWGG 8 cut(s) 103, 321, 454, 558, 874, 976, 2251, 2334
PspN4I GGNNCC 3 cut(s) 564, 911, 1345
PspPI GGNCC 4 cut(s) 458, 845, 1414, 2255
PspPPI RGGWCCY 1 cut(s) 2255
PstI CTGCAG 2 cut(s) 1279, 1624
PstNI CAGNNNCTG 1 cut(s) 2258
PvuII CAGCTG 1 cut(s) 1613
RsaI GTAC 9 cut(s) 42, 488, 525, 628, 1193, 1785, 1909, 2236, 2244
RsaNI GTAC 9 cut(s) 41, 487, 524, 627, 1192, 1784, 1908, 2235, 2243
RseI CAYNNNNRTG 4 cut(s) 956, 1470, 1674, 2198
SatI GCNGC 4 cut(s) 87, 90, 1407, 1611
Sau3AI GATC 6 cut(s) 18, 508, 1423, 1574, 1963, 2309
Sau96I GGNCC 4 cut(s) 458, 845, 1414, 2255
ScaI AGTACT 3 cut(s) 42, 525, 1785
SchI GAGTC 2 cut(s) 613, 1329
ScrFI CCNGG 9 cut(s) 105, 323, 456, 560, 876, 978, 1412, 2253, 2336
SduI GDGCHC 2 cut(s) 1560, 1630
SfaNI GCATC 7 cut(s) 98, 972, 1050, 1907, 1938, 2131, 2176
SfcI CTRYAG 4 cut(s) 423, 1275, 1620, 1983
SinI GGWCC 1 cut(s) 2255
SmiMI CAYNNNNRTG 4 cut(s) 956, 1470, 1674, 2198
SmlI CTYRAG 1 cut(s) 1286
SmoI CTYRAG 1 cut(s) 1286
SnaBI TACGTA 1 cut(s) 248
SphI GCATGC 1 cut(s) 801
SsiI CCGC 3 cut(s) 445, 482, 614
SspI AATATT 1 cut(s) 1817
SspMI CTAG 6 cut(s) 639, 1367, 1631, 1787, 1890, 2096
StyD4I CCNGG 9 cut(s) 103, 321, 454, 558, 874, 976, 1410, 2251, 2334
StyI CCWWGG 4 cut(s) 492, 1324, 1944, 2126
TaaI ACNGT 4 cut(s) 164, 424, 1078, 1348
TaiI ACGT 4 cut(s) 250, 300, 1854, 2202
TaqI TCGA 4 cut(s) 900, 1030, 1672, 1806
TatI WGTACW 6 cut(s) 40, 523, 1783, 1907, 2234, 2242
TfiI GAWTC 3 cut(s) 136, 889, 2331
TscAI CASTG 2 cut(s) 125, 427
TseFI GTSAC 5 cut(s) 158, 197, 440, 1740, 1847
TseI GCWGC 4 cut(s) 86, 89, 1406, 1610
Tsp45I GTSAC 5 cut(s) 158, 197, 440, 1740, 1847
TspGWI ACGGA 1 cut(s) 350
TspRI CASTG 2 cut(s) 125, 427
VpaK11BI GGWCC 1 cut(s) 2255
VspI ATTAAT 2 cut(s) 840, 1748
XagI CCTNNNNNAGG 1 cut(s) 269
XapI RAATTY 4 cut(s) 182, 1134, 1266, 1968
XceI RCATGY 1 cut(s) 801
XcmI CCANNNNNNNNNTGG 1 cut(s) 2080
XspI CTAG 6 cut(s) 639, 1367, 1631, 1787, 1890, 2096
ZrmI AGTACT 3 cut(s) 42, 525, 1785
Zsp2I ATGCAT 1 cut(s) 799
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.